Effect of Electroacupuncture on 26 S Proteasome and Nuclear Factor kappa B in Substantia Nigra of Rats with Rotenone-induced Parkinson's Disease

TU Qian; LIANG Yan; MA Jun; WANG Shu-ju; SHEN Feng; WANG Yan-chun

doi:10.13702/j.1000-0607.2015.04.001

您当前的位置：

首页 >

文章列表页 >

Effect of Electroacupuncture on 26 S Proteasome and Nuclear Factor kappa B in Substantia Nigra of Rats with Rotenone-induced Parkinson's Disease

更新时间：2023-08-11

- Effect of Electroacupuncture on 26 S Proteasome and Nuclear Factor kappa B in Substantia Nigra of Rats with Rotenone-induced Parkinson's Disease
- Acupuncture Research Vol. 40, Issue 4, Pages: 259-264(2015)
- 作者机构：
  
  1. 湖北中医药大学针灸骨伤学院
  2. 江汉大学医学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2015.04.001
  CLC：
- Published：2015
- 稿件说明：
移动端阅览
TU Qian, LIANG Yan, MA Jun, et al. Effect of Electroacupuncture on 26 S Proteasome and Nuclear Factor kappa B in Substantia Nigra of Rats with Rotenone-induced Parkinson's Disease[J]. Acupuncture research, 2015, 40(4): 259-264.
DOI：

TU Qian, LIANG Yan, MA Jun, et al. Effect of Electroacupuncture on 26 S Proteasome and Nuclear Factor kappa B in Substantia Nigra of Rats with Rotenone-induced Parkinson's Disease[J]. Acupuncture research, 2015, 40(4): 259-264. DOI： 10.13702/j.1000-0607.2015.04.001.

摘要

目的:探讨26S蛋白酶体、核转录因子κB(NFκB)在电针对鱼藤酮致帕金森病(Parkinson’s disease

PD)模型大鼠保护机制中的作用。方法:将48只健康雄性SD大鼠随机分为正常组、假手术组、模型组、电针组

每组12只。模型组和电针组采用颈背部皮下注射鱼藤酮法造模40d。造模成功后电针组取"风府""太冲"行电针治疗(连续波

2Hz

1mA

20min/次

1次/d

连续28d)。观察各组大鼠行为学改变并评分

应用免疫组化法观察大鼠中脑黑质酪氨酸羟化酶(tyrosine hydroxylase

TH)的表达

免疫蛋白印迹法检测黑质TH、26S蛋白酶体、NFκB表达。结果:与正常组及假手术组比较

模型组大鼠出现明显的行为学改变

电针组行为学评分较模型组显著降低(P<0.05);与正常组及假手术组比较

模型组大鼠中脑黑质TH阳性神经元细胞计数、含量明显降低

电针组TH阳性细胞数、含量较模型组明显升高(均P<0.05);26S蛋白酶体在模型组表达较正常组及假手术组明显下降

电针组与模型组比较明显升高(均P<0.05);NFκB在模型组表达较正常组及假手术组明显增高

电针组与模型组比较明显降低(均P<0.05)。结论:电针显著改善鱼藤酮所致PD模型大鼠的行为学表现和评分

显著改善鱼藤酮所致黑质多巴胺能神经元丢失

从而发挥防治PD作用

其可能机制与增强蛋白酶体系统的功能有关

与其降低NFκB表达有关。

Abstract

Objective To explore the role of 26 Sproteasome and nuclear factor kappa B(NFκB)in substantia nigra in the management of Parkinson's disease(PD)by acupuncture.Methods Forty-eight male SD rats were randomly divided into normal

sham operation

model and electroacupuncture(EA)groups(12rats/group).The PD model was established by 40-day consecutive subcutaneous injection of rotenone(1mg/kg dissolved in dimethyl sulfoxide and normal saline)at the back shoulder.The rats in the sham operation group were treated by subcutaneous injection of dose of saline."Fengfu"(GV 16)and"Taichong"(LR 3)acupoints were stimulated with EA at 2Hz

1mA

20 min of duration in each treatment

and daily for 28 consecutive days.The behavioral changes of rats in each group were measured and scored at 40 th day and 68 th day

respectively.Immunohistochemistry was used to determine the expression of tyrosine hydroxylase(TH)used to detect the expression of 26 Sproteasome and NFκB and TH were measured by Western blot.Results In comparison with the normal and sham operation groups

the behavioral scores of rats in the model group were elevated

which were significantly decreased by EA intervention(P<0.05).The expression of TH and 26 Sproteasome decreased whereas the NFκB increased in the rats of model group(P<0.05)

and EA intervention reversed these changes(all P<0.05).Conclusion EA intervention can improve PD rats' behavioral changes

which is pobably related to its effects in rcducing loss of TH-positive neurons

down-regulating NFκB protein expression

and upregulating 26 Sproteasome protein expression in the substantia nigra.

关键词

Keywords

references

Views

357

下载量

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of electroacupuncture against intestinal inflammation in mice with Parkinson’s disease by NF-κB/IL-6 pathway

Effect of electroacupuncture on GLP-1R/PI3K/Akt protein pathway in mice with Parkinson’s disease

Effect of Electroacupuncture Intervention on ERK 1/2 Signaling and TNF-αand IL-1β Protein Levels in the Substantia Nigra in Rats with Parkinson's Disease

Effect of Electroacupuncture Stimulation of "Fengfu"(GV 16) and "Taichong"(LR 3) on Expression of COX-2 and Tyrosine Hydroxylase in Substantia Nigra in Rats with Parkinson's Di-sease

Clinical efficacy and safety of acupuncture therapy for central facial palsy after stroke： a systematic review and Meta-analysis

Related Author

WANG Yao

MA Jun

WANG Yan-chun

GUAN Qing-ya

LI Han-zhang

QI Ling

ZHANG Xiao-lei

CHEN Xiang-lin

Related Institution

College of Acupuncture and Orthopedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion

College of Traditional Chinese Medicine, Hubei University of Chinese Medicine

Zhongjing Institute of Traditional Chinese Medicine, Henan University of Chinese Medicine

College of Acupuncture and Orthopaedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion

College of Acu-moxibustion and Orthopedics,Hubei University of Chinese Medicine

Postal code：100700
Tel：010-64089344,84014607 Email：zcyjbjb@vip.163.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05017590号-8 京公网安备11010802020460号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰