Objective To observe the influence of acupuncture intervention on the expression of pivotal pro-inflammatory molecules nuclear factor-κB(NF-κB)

cyclooxygenase-2(COX-2)

and prostaglandin E2(PGE2)of NF-κB inflammatory signal pathway in the hippocampus of depression rats

so as to explore its mechanism underlying improvement of depression.Methods Thirty-six male Sprague-Dawley rats were randomly divided into four groups:control

model

acupuncture

and medication(fluoxetine

Flu)(n=9rats in each group).The depression model was established by using chronic unpredictable stress stimuli combined with solitary feeding for 28 days.Acupuncture treatment was applied to"Baihui"(GV 20)and"Neiguan"(PC 6)once every other day for 28 days.Fluoxetine was given to rats of the medication group(10mg/kg)by gavage(p.o.)everyday for 28 days before the stress stimulation.The expression of NF-κB protein in the hippocampus tissue was detected by Western blot

and the contents of COX-2and PGE2 were detected by ELISA.Results Compared to the control group

the levels of NF-κB protein expression

COX-2and PGE2 contents in the hippocampus were significantly increased in the model group(P<0.01).Compared to the model group

the levels of NF-κB protein expression

COX-2and PGE2 contents in the hippocampus were significantly downregulated in both the acupuncture and medication groups(P<0.05

P<0.01).There was no significant differences between the acupuncture and medication groups in the NF-κB protein expression

and COX-2and PGE2contents(P>0.05).ConclusionAcupuncture intervention can effectively reduce hippocampal NF-κB expression

and COX-2and PGE2 levels in depression rats

which may contribute to its anti-depressant effect by inhibiting pro-inflammatory pathway to protect the neurons of hippocampus.