Effect of Electroacupuncture Intervention at Different Time-points on Levels of HSP 70,MDA,SOD and GSH-PX of Liver in Rats with Simulated Weightlessness

SONG Yan; ZHAO Guo-zhen; ZHAO Bai-xiao; JI Bo; WANG De-sheng; ZHANG He; MAO Ying-qiu; ZHANG Ping; XU Yong-si; LIU Ya-li; LU Ya-wen; DAI Jian; LI Ying-hui

doi:10.13702/j.1000-0607.2015.05.008

您当前的位置：

首页 >

文章列表页 >

Effect of Electroacupuncture Intervention at Different Time-points on Levels of HSP 70,MDA,SOD and GSH-PX of Liver in Rats with Simulated Weightlessness

更新时间：2023-08-11

- Effect of Electroacupuncture Intervention at Different Time-points on Levels of HSP 70,MDA,SOD and GSH-PX of Liver in Rats with Simulated Weightlessness
- Acupuncture Research Vol. 40, Issue 5, Pages: 383-387(2015)
- 作者机构：
  
  1. 北京中医药大学
  2. 首都医科大学
  3. 中国航天员科研训练中心
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2015.05.008
  CLC：
- Published：2015
- 稿件说明：
移动端阅览
SONG Yan, ZHAO Guo-zhen, ZHAO Bai-xiao, et al. Effect of Electroacupuncture Intervention at Different Time-points on Levels of HSP 70,MDA,SOD and GSH-PX of Liver in Rats with Simulated Weightlessness[J]. Acupuncture research, 2015, 40(5): 383-387.
DOI：

SONG Yan, ZHAO Guo-zhen, ZHAO Bai-xiao, et al. Effect of Electroacupuncture Intervention at Different Time-points on Levels of HSP 70,MDA,SOD and GSH-PX of Liver in Rats with Simulated Weightlessness[J]. Acupuncture research, 2015, 40(5): 383-387. DOI： 10.13702/j.1000-0607.2015.05.008.

摘要

目的:观察预针刺和针刺治疗对模拟失重大鼠肝脏超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活力

丙二醛(MDA)含量及热休克蛋白70(HSP 70)表达的影响

探讨电针对模拟失重大鼠肝脏损伤改善作用的相关机制。方法:Wistar大鼠随机分为正常组、模型组、预针刺组和针刺组

每组5只。大鼠尾部悬吊4周复制模拟失重模型。预针刺组在尾部悬吊前1周

电针刺激双侧"肾俞""脾俞"和"三阴交"穴

每次30min

每日1次;针刺组大鼠尾部悬吊过程中电针刺激"肾俞""脾俞"和"三阴交"穴

每次30min

隔日治疗1次

共针刺14次。采用免疫组化法检测大鼠肝脏组织HSP 70的表达情况

比色法检测大鼠肝脏组织SOD、GSH-PX活性和MDA含量。结果:与正常组比较

模型组大鼠肝脏HSP 70呈强阳性反应

HSP 70表达量显著增加(P<0.01)

MDA含量显著升高(P<0.01)

SOD和GSH-PX活性显著降低(P<0.05);与模型组比较

预针刺组大鼠肝脏HSP 70表达量显著降低(P<0.01)

SOD和GSH-PX活性略有升高(P>0.05)

MDA含量略有减少(P>0.05)

针刺组HSP 70表达量略有降低(P>0.05)

SOD活性和MDA含量略有升高(P>0.05)

GSH-PX活性略有降低(P>0.05);与预针刺组相比

针刺组肝脏GSH-PX活性显著降低(P<0.05)

MDA含量显著升高(P<0.05)。结论:预针刺可以明显抑制大鼠尾部悬吊引起的肝HSP 70表达的上调

从而可能有利于提高肝脏的抗氧化能力。

Abstract

Objective To observe the effect of acupuncture intervention at different time-points on the activity of superoxide dismutase(SOD)

glutathione peroxidase(GSH-PX)

the content of malonaldehyde(MDA)and expression of heat shock protein 70(HSP 70)of liver tissue in rats with simulated weightlessness

so as to explore its mechanism underlying improvement of liver injury in rats with simulated weightlessness.Methods Twenty male Wistar rats were randomly divided into control group

model group

pre-acupuncture group and EA group

5rats in each.The model of simulated weightlessness was established by tail suspension for 4week.One week before the tail suspension

the rats in the pre-acupuncture group were treated with electroacupuncture(EA)at"Shenshu"(BL 23)

"Pishu"(BL 20)and "Sanyinjiao"(SP 6)for 30 min before treatment

once a day for 7days.The rats in the EA group received tail suspension and acupuncture intervention at the same time.EA was applied for 30 min per treatment

once every other day for 14 times.Immunohistochemical staining was used to assay the expression of HSP 70 in the liver tissue.The activities of SOD and GSH-PX and content of MDA in liver tissues were examined by means of colourimetric method.Results Compared with the control group

the expression of HSP 70 and the content of MDA in the liver tissue were increased significantly(P<0.01)

and the activity of SOD and GSH-PX was notably reduced(P<0.05)in the model group.Compared with the model group

the content of HSP 70 was significantly reduced in the pre-acupuncture group(P<0.01).There were no significant changes in the levels of SOD

GSH-PX

MDA and HSP 70 in the EA group(P>0.05).In comparison with the pre-acupuncture group

the activity of GSH-PX was lower(P<0.05)and the content of MDA was higher(P<0.05)in the EA group.Conclusion EA-pretreatment can suppress the increase of liver HSP 70 immunoactivity in rats with simulated weightlessness

being likely to improve the antioxidant ability of liver.

关键词

Keywords

references

Views

330

下载量

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Acupuncture on Serum Malonaldehyde Content,Superoxide Dismutase and Glutathione Peroxidase Activity in Chronic Fatigue Syndrome Rats

Effect of different acupuncture and moxibustion methods on uterine contraction and microcirculation in cold congealing dysmenorrhea rats

Research progress on the mechanism of acupuncture based on microbiota-gut-brain axis

Research progress on the effect of α-synuclein in acupuncture treatment for Parkinson’s disease

Advances of researches on acupuncture treatment of Parkinson’s disease by regulating mitochondrial function

Related Author

LIU Chang-zheng

LEI Bo

TIAN Yuan

MA Liang-xiao

YU Wen-yan

MU Jie-dan

ZHANG Zhou

SUN Tian-yi

Related Institution

School of Aesthetic Medicine,Yichun College

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine

The Key Unit of State Administration of Traditional Chinese Medicine, Evaluation of Characteristic Acupuncture Therapy

Institute of Acupuncture and Moxibustion， China Academy of Chinese Medical Sciences

The Second Clinical Medical School， Heilongjiang University of Traditional Chinese Medicine

Postal code：100700
Tel：010-64089344,84014607 Email：zcyjbjb@vip.163.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05017590号-8 京公网安备11010802020460号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰