Effects of Electroacupuncture Stimulation of“Xiajuxu”(ST 39),etc.on Duodenal Mucosal Injury,Serum Pro-inflammatory Factors Levels and Duodenal Nicotinic Acetylcholine Receptor α7 Expression in Duodenal Ulcer Rats

LING Xi; ZHANG Hong; YI Xi-qin; WU Jin-feng

doi:10.13702/j.1000-0607.2016.02.003

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Effects of Electroacupuncture Stimulation of“Xiajuxu”(ST 39),etc.on Duodenal Mucosal Injury,Serum Pro-inflammatory Factors Levels and Duodenal Nicotinic Acetylcholine Receptor α7 Expression in Duodenal Ulcer Rats

更新时间：2023-08-11

- Effects of Electroacupuncture Stimulation of“Xiajuxu”(ST 39),etc.on Duodenal Mucosal Injury,Serum Pro-inflammatory Factors Levels and Duodenal Nicotinic Acetylcholine Receptor α7 Expression in Duodenal Ulcer Rats
- Acupuncture Research Vol. 41, Issue 2, Pages: 108-112(2016)
- 作者机构：
  
  湖南中医药大学针灸推拿学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2016.02.003
  CLC：
- Published：2016
- 稿件说明：
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LING Xi, ZHANG Hong, YI Xi-qin, et al. Effects of Electroacupuncture Stimulation of“Xiajuxu”(ST 39),etc.on Duodenal Mucosal Injury,Serum Pro-inflammatory Factors Levels and Duodenal Nicotinic Acetylcholine Receptor α7 Expression in Duodenal Ulcer Rats[J]. Acupuncture research, 2016, 41(2): 108-112.
DOI：

LING Xi, ZHANG Hong, YI Xi-qin, et al. Effects of Electroacupuncture Stimulation of“Xiajuxu”(ST 39),etc.on Duodenal Mucosal Injury,Serum Pro-inflammatory Factors Levels and Duodenal Nicotinic Acetylcholine Receptor α7 Expression in Duodenal Ulcer Rats[J]. Acupuncture research, 2016, 41(2): 108-112. DOI： 10.13702/j.1000-0607.2016.02.003.

摘要

目的:对比观察电针十二指肠溃疡(DU)模型大鼠胃肠胆腑下合穴"下巨虚""足三里""上巨虚""阳陵泉"等穴后对血清及十二指肠组织炎性反应相关物质的影响

探讨小肠下合穴"下巨虚"治疗对应腑病是否存在相对特异性。方法:SD大鼠随机分为空白、模型、下巨虚、足三里、上巨虚、阳陵泉组

每组10只。除空白组外均皮下注射盐酸半胱胺建立DU模型

各穴位组取相应穴位进行电针治疗

每日1次

共治疗10d后

肉眼观察大鼠十二指肠黏膜溃疡情况

ELISA法检测大鼠血清中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)及高迁移率族蛋白B 1(HMGB 1)含量

Western blot法检测大鼠十二指肠组织烟碱型乙酰胆碱受体α7(α7nAchR)的表达。结果:与空白组比较

模型组肉眼溃疡评分增高

血清TNF-α、IL-1β及HMGB 1含量均增高(P<0.01)

十二指肠中α7nAchR表达水平降低(P<0.01)。与模型组比较

上巨虚、下巨虚、足三里组肉眼溃疡评分依次降低(P<0.05

P<0.01)

4个穴位组TNF-α、IL-1β及HMGB 1含量均不同程度降低(P<0.01

除阳陵泉组IL-1β外)

而足三里、下巨虚组α7nAchR表达水平增高(P<0.01

P<0.05)。与下巨虚组比较

上巨虚组TNF-α含量增高(P<0.01);阳陵泉组肉眼溃疡评分及TNF-α、IL-1β、HMGB 1含量亦增高(P<0.01

P<0.05)

α7nAchR表达水平降低(P<0.05)。与足三里组比较

上巨虚组TNF-α、HMGB 1含量增高(P<0.01

P<0.05);阳陵泉组肉眼溃疡评分及TNF-α、IL-1β、HMGB1含量亦增高(P<0.05

P<0.01)

α7nAchR表达水平降低(P<0.05)。结论:1针刺胃肠胆腑下合穴均可通过抑制机体免疫应答

减轻炎性反应

降低黏膜损伤来实现对DU大鼠的干预作用;2下巨虚穴与对应小肠腑之间存在相对特异性联系;3下巨虚、足三里二穴的总体效应基本一致

提示二穴的靶器官效应可能存在部分交集。

Abstract

Objective To observe the relatively specific effect of electroacupuncture(EA)of"Xiajuxu"(ST 39

the lower he-sea point of the small intestine)

etc.on the level of serum TNF-α

Interleukin-1β(IL-1β)and high mobility group protein B 1(HMGB 1)contents

and duodenumα7nicotinic acetylcholine receptor(nAchR)expression in duodenal ulcer rats

so as to explore its mechanisms underlying improving duodenal ulcer.Methods Sixty SD rats were randomly divided into 6groups:normal control

model

Xiajuxu(ST 39)

Zusanli(ST 36)

Shangjuxu(ST 37)and Yanglingquan(GB 34).The duodenal ulcer model was established by subcutaneous injection of 10% Cysteamine Hydrochloride(300mg/kg)

following by giving the rats with access to water containing Cysteamine.EA(10Hz/50 Hz

1-3mA)was applied to bilateral ST 39

ST 36

ST 37 and GB 34for30 min

once daily for 10 days.The ulcer scores(0-5points)of the duodenal mucosa were assessed according to modified Moraes' methods.Serum TNF-α

IL-1βand HMGB 1levels were assayed by ELISA and the expression of neuronalα7nAchR in the duodenal tissue was detected by Western blot.Results After modeling

the ulcer score

serum TNF-α

IL-1βand HMGB 1contents were significantly increased(P<0.01)and the expression level ofα7nAchR in the duodenal tissue was significantly downregulated in comparison with the normal control group(P<0.01).Following EA intervention

the serum TNF-αand HMGB 1contents in the Xiajuxu(ST 39)

Zusanli(ST 36)

Shangjuxu(ST 37)and Yanglingquan(GB 34)groups

and the ulcer scores and IL-1βcontent of the Xiajuxu(ST 39)

Zusanli(ST 36)and Shangjuxu(ST 37)groups were considerably reduced

and the expression ofα7nAchR in both Xiajuxu(ST 39)and Zusanli(ST 36)groups was evidently increased(P<0.05

P<0.01).No significant changes were found in the ulcer score

serum IL-1βcontent

andα7nAchR expression in the Yanglingquan(GB 34)group andα7nAchR expression in the Shangjuxu(ST 37)group in comparison with the model group(P>0.05).Conclusion EA stimulation of ST 36

ST 37 and ST 39 can reduce ulcer injury in duodenal ulcer model rats

which may be associated with their effects in down-regulating serum TNF-α

IL-1βand HMGB 1contents and up-regulatingα7nAchR expression of the duodenal tissue

possibly by suppressing immune and inflammatory reactions and regulating nicotinic activity.

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Related Institution

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