JIANG Xi-rong, REN Lu, LI Chun-ri. Effect of Electroacupuncture on Hypothalamus-Pituitary-Ovary(HPO)Axis in Rats with Perimenopausal Depression[J]. Acupuncture research, 2017, 42(1): 45-49.
DOI:
JIANG Xi-rong, REN Lu, LI Chun-ri. Effect of Electroacupuncture on Hypothalamus-Pituitary-Ovary(HPO)Axis in Rats with Perimenopausal Depression[J]. Acupuncture research, 2017, 42(1): 45-49. DOI: 10.13702/j.1000-0607.2017.01.008.
Effect of Electroacupuncture on Hypothalamus-Pituitary-Ovary(HPO)Axis in Rats with Perimenopausal Depression
Objective To observe the effect of electroacupuncture(EA)on the hormones derived from the hypothalamus-pituitary-ovary(HPO)axis
so as to explore the neuroendocrine mechanism induced by EA on rats with perimenopausal depression disorder.Methods Sixty female sprague-dawley rats were randomly divided into blank control group
model group
sham-operation(sham)group
clomipramine group
and electroacupuncture(EA)group
with 12 rats in each group.Perimenopausal depression model was established by bilateral ovariectomy combined with chronic unpredictable stimulation.The EA group received continuous treatment at"Baihui"(GV 20)
"Shenshu"(BL 23)and "Sanyinjiao"(SP 6)once a day for 28 days.Estrous cycle and sucrose preference test were monitored
and serum estradiol(E2)
luteinizing hormone(LH)
gonadotropin releasing hormone(GnRH)
andβ-endorphin(β-EP)were detected by ELISA.Results Compared to the blank control group
sugar water consumpution rates decreased in the model group and sham group(P<0.05).Compared to the blank group and sham group
the serum LH and GnRH levels increased(P<0.05)
companied with lower serum E2andβ-EP levels in the model group(P<0.05).Compared to the model group
sugar water consumpution rates increased in the clomipramine group and EA group(P<0.05)
both were companied with decreased serum LH and GnRH levels(P<0.05)
and higher serum E2andβ-EP levels(P<0.05).Conclusion Electroacupuncture can relieve the symptoms of rat with perimenopausal depression by regulating the hormone secretion in HPO axis.
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