Experimental Study for the Treatment of Asthenozoospermia by Electroacupuncture in Rats

JIN Zi-run; LIU Bo-heng; CAI Jie; JING Xiang-hong; ZHU Bing; XING Guo-gang

doi:10.13702/j.1000-0607.2017.02.004

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Experimental Study for the Treatment of Asthenozoospermia by Electroacupuncture in Rats

更新时间：2023-08-11

- Experimental Study for the Treatment of Asthenozoospermia by Electroacupuncture in Rats
- Acupuncture Research Vol. 42, Issue 2, Pages: 114-118(2017)
- 作者机构：
  
  1. 北京大学神经科学研究所、北京大学基础医学院神经生物学系
  2. 中国中医科学院针灸研究所
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2017.02.004
  CLC：
- Published：2017
- 稿件说明：
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JIN Zi-run, LIU Bo-heng, CAI Jie, et al. Experimental Study for the Treatment of Asthenozoospermia by Electroacupuncture in Rats[J]. Acupuncture research, 2017, 42(2): 114-118.
DOI：

JIN Zi-run, LIU Bo-heng, CAI Jie, et al. Experimental Study for the Treatment of Asthenozoospermia by Electroacupuncture in Rats[J]. Acupuncture research, 2017, 42(2): 114-118. DOI： 10.13702/j.1000-0607.2017.02.004.

摘要

目的:研究不同电针参数对大鼠弱精子症的治疗作用。方法:将105只雄性SD大鼠随机分为2Hz-每日电针组9只、假电针组10只、模型组10只

2Hz-隔日电针5d组、假电针组、模型组各8只

2Hz-隔日电针9d组、假电针组、模型组各10只

100Hz-隔日电针组7只、假电针组8只、模型组7只。采用奥硝唑灌胃法建立弱精子症大鼠模型。各电针组电针双侧"足三里""肾俞"穴

各假电针组予常规针刺。2Hz-每日电针组

每日治疗1次

共3次;2Hz-隔日电针5d组

隔天治疗1次

共3次;2Hz-隔日电针9d组

隔天治疗1次

共5次;100Hz-隔日电针组

隔天治疗1次

共5次。通过计算机辅助精子分析系统观察电针对精子密度、活率、活力、A级精子数以及A+B级精子数的影响。结果:2Hz-每日电针组:与模型组及假电针组相比

2Hz-每日电针治疗对弱精子症大鼠精子运动的各项指标均没有明显改善作用(P>0.05)。2 Hz-隔日电针5d组:与模型组相比

2Hz-隔日电针5d治疗可以增加大鼠精子的活力、A级精子数以及A+B级精子数(P<0.05);而与假电针组相比

2 Hz-隔日电针5d治疗只增加了A+B级精子数(P<0.05)。2Hz-隔日电针9d组:与模型组及假电针组相比

电针后弱精子症大鼠精子的活率、活力、A级精子数、A+B级精子数均显著升高(P<0.001)。100Hz-隔日电针组:与模型组及假电针组相比

100Hz-隔日电针5次治疗可提高大鼠精子的活率(P<0.001

P<0.05)、活力(P<0.001

P<0.01)、A级精子数及A+B级精子数(P<0.01)。各电针参数对弱精子症大鼠的精子密度无明显影响(P>0.05)。结论:2Hz-隔日电针9d或100Hz-隔日电针9d都可以提高弱精子症大鼠的精子活率和活力

提升精子运动能力

对弱精子症有一定的治疗作用。

Abstract

Objective To investigate the effects of different electroacupuncture(EA)parameters for the treatment of asthenozoospermia in rats.Methods One hundred and five male Sprague-Dawley rats were randomly divided into 2 Hz-EA treatment daily in 3dgroup(n=9)

sham-EA group(n=10)

model group(n=10);2Hz-EA treatment every other day in 5dgroup

sham-EA group

model group(8rats in each group);2Hz-EA treatment every other day in 9dgroup

sham-EA group

model group(10rats in each group);100Hz-EA treatment every other day in 9dgroup(n=7)

sham-EA group(n=8)

model group(n=7).Asthenozoospermia model was established by intragastric administration of ornidazole(ORN

400mg·kg-1·d-1)once daily till the end of treatment.EA treatments(2Hz or 100Hz)were applied to"Shenshu"(BL 23

bilateral)

"Zusanli"(ST 36

bilateral)for 30 min

intensity of 1-2-3mA(increasing 1mA per 10min)

once a day or once every other day for 3times or 5times.Sham-EA groups were treated with similar procedure except that the output leads of the stimulator were disconnected.The sperm density

viability

motility

the number of grade A sperm

and grade A+B sperm were examined by computer-assisted sperm analysis.Results(1)2Hz-EA treatment daily in 3d:compared with the model group and the sham-EA group

2Hz-EA treatment once daily had no significant effect on all of the sperm motility indexes in the asthenozoospermic rats(P>0.05).(2)2Hz-EA treatment every other day in 5d:compared with the model group

EA treatment could increase the sperm motility(P<0.05)

the number of grade A sperm(P<0.05)

and the number of grade A+B sperm(P<0.05)in the asthenozoospermic rats.However

compared with the sham-EA group

EA treatment could only improve the number of grade A+B sperm(P<0.05).(3)2Hz-EA treatment every other day in 9d:compared with both the model group and the sham-EA group

EA treatment could markedly improve the sperm viability(P<0.001)

the sperm motility(P<0.001)

the number of grade A sperm(P<0.001)

and the number of grade A+B sperm(P<0.001)in the asthenozoospermic rats.(4)100Hz-EA treatment every other day in 9days:compared with both the model group and the sham-EA group

all of the sperm indexes in the asthenozoospermic rats including the sperm viability(P<0.001 vs.the model group

P<0.05 vs.the sham-EA group)

the sperm motility(P<0.001 vs.the model group

P<0.01 vs.the sham-EA group)

the number of grade A sperm(P<0.01)and the number of grade A+B sperm(P<0.01)also could be improved after EA treatment.Unexpectedly

none of the EA treatment had significant influence on the sperm density in the asthenozoospermic rats.Conclusion Both 2Hz-EA and 100Hz-EA treatment once every other day for 5times in 9dhad a therapeutic effect on asthenozoospermia by improving the sperm viability and the sperm motility in the rats.

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