ZHANG Yuan, HOU Xun-rui, LI Li-hong, et al. Effect of Acupoint Injection on Eosinophil Counts,Protein and mRNA Expressions of Eotaxin in Nasal Mucosa of Allergic Rhinitis Rats[J]. Acupuncture research, 2017, 42(2): 141-144.
DOI:
ZHANG Yuan, HOU Xun-rui, LI Li-hong, et al. Effect of Acupoint Injection on Eosinophil Counts,Protein and mRNA Expressions of Eotaxin in Nasal Mucosa of Allergic Rhinitis Rats[J]. Acupuncture research, 2017, 42(2): 141-144. DOI: 10.13702/j.1000-0607.2017.02.009.
Effect of Acupoint Injection on Eosinophil Counts,Protein and mRNA Expressions of Eotaxin in Nasal Mucosa of Allergic Rhinitis Rats
Objective To observe the effect of acupoint injection on eosinophils(EOS)counts and expression of eotaxin in nasal mucosa of allergic rhinitis(AR)rats
so as to reveal its mechanism underlying improving AR.Methods Twenty-four Sprague-Dawley(SD)rats were randomly divided into normal
model and acupoint injection groups(n=8in each group).The AR model was established by intraperitoneal injection of ovalbumin sensitization.Bilateral"Yingxiang"(LI 20)and"Yintang"(GV29)were selected for acupoint injection of the mixture solution of lidocaine
dexamethasone
and transfer factor(0.1mL/acupoint)on the 1st
5th
9th
and 13 th day after AR model established
a total of four times.EOS in the nasal mucosa was counted under light microscope after HE staining.Protein and mRNA expressions of eotaxin in the nasal mucosa were detected by immunohistochemical and RT-PCR methods
respectively.Results Compared with the normal group
EOS counts
protein and mRNA expressions of eotaxin in the nasal mucosa were significantly higher in the model group(P<0.05).Compared with the model group
EOS counts
protein and mRNA expressions of eotaxin in the nasal mucosa were significantly lower in the acupoint injection group(P<0.05).Conclusion Acupoint injection can reduce the nasal mucosa inflammation by suppressing the protein and mRNA expressions of eotaxin
decreasing the infiltration and gathering of EOS in the nasal mucosa.
Acupoint injection inhibiting abnormal secretion of mucin and inflammatory reaction of nasal mucosa in rats with allergic rhinitis through the p38 mitogen-activated protein kinase pathway
Acupoint injection ameliorates Th1/Th2 imbalance through Toll-like receptor 4/activator protein-1 signal pathway and improves inflammatory response in rats with allergic rhinitis
Synergistic effect of acupoint injection and moxibustion or catgut embedding or acupuncture on symptoms and expression of Th1/Th2 related cytokines in nasal mucosa of rats with allergic rhinitis
Acupoint injection improves allergic rhinitis by balancing Th17/Treg in allergic rhinitis rats
Related Author
ZHOU Yu-ling
WANG Yu-jia
ZHANG Qian
HOU Xun-rui
ZHOU Ke-an
LI Li-hong
LI Li-hong
ZHANG Qian
Related Institution
The Affiliated Hospital of Guizhou Medical University
Clinical Medical College, Guizhou Medical University
the Affliated Hospital,Guizhou Medical University
Zunyi Municipal Hospital of Traditional Chinese Medicine
Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine