Effect of Electroacupuncture on Expression of Catechol-O-methyltransferase in the Inferior Colliculus and Auditory Cortex in Age-related Hearing Loss Guinea Pigs

LIU Shu-yun; DENG Li-qiang; YANG Ye; YIN Ze-deng

doi:10.13702/j.1000-0607.2017.02.010

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Effect of Electroacupuncture on Expression of Catechol-O-methyltransferase in the Inferior Colliculus and Auditory Cortex in Age-related Hearing Loss Guinea Pigs

更新时间：2023-08-11

- Effect of Electroacupuncture on Expression of Catechol-O-methyltransferase in the Inferior Colliculus and Auditory Cortex in Age-related Hearing Loss Guinea Pigs
- Acupuncture Research Vol. 42, Issue 2, Pages: 145-148(2017)
- 作者机构：
  
  1. 西南医科大学附属医院耳鼻咽喉头颈外科
  2. 湖南省郴州市第一人民医院儿童耳鼻喉科
  3. 西南医科大学基础医学院生物化学教研室
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2017.02.010
  CLC：
- Published：2017
- 稿件说明：
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LIU Shu-yun, DENG Li-qiang, YANG Ye, et al. Effect of Electroacupuncture on Expression of Catechol-O-methyltransferase in the Inferior Colliculus and Auditory Cortex in Age-related Hearing Loss Guinea Pigs[J]. Acupuncture research, 2017, 42(2): 145-148.
DOI：

LIU Shu-yun, DENG Li-qiang, YANG Ye, et al. Effect of Electroacupuncture on Expression of Catechol-O-methyltransferase in the Inferior Colliculus and Auditory Cortex in Age-related Hearing Loss Guinea Pigs[J]. Acupuncture research, 2017, 42(2): 145-148. DOI： 10.13702/j.1000-0607.2017.02.010.

摘要

目的:观察D-半乳糖所致的年龄相关性聋(AHL)豚鼠下丘和听皮层儿茶酚氧位甲基转移酶(COMT)表达

探讨电针防治AHL的可能机制。方法:3月龄豚鼠随机分为对照组、模型组、电针组

每组10只

另10只18月龄豚鼠为老年组。以D-半乳糖颈背部皮下注射复制AHL模型。电针组造模同时予以电针"翳风"穴和"听宫"穴

每日1次

每次15min

共6周。治疗后测定各组听性脑干反应(ABR)Ⅲ波潜伏期

Western blot法检测各组下丘及听皮层COMT的表达。结果:与对照组比较

模型组和老年组ABRⅢ波潜伏期显著延长(P<0.05);与模型组比较

电针组ABRⅢ波潜伏期显著缩短(P<0.05)。与对照组比较

模型组和老年组下丘和听皮层COMT表达显著降低(P<0.05);与模型组比较

电针组下丘和听皮层COMT表达显著增加(P<0.05)。结论:电针"翳风"穴和"听宫"穴能改善AHL豚鼠的听力

其作用机制可能与增加下丘和听皮层COMT表达有关。

Abstract

Objective To observe the expression of catechol-O-methyltransferase(COMT)in inferior colliculus and auditory cortex of guinea pigs with age-related hearing loss(AHL)induced by D-galactose

so as to explore the possible mechanism of electroacupuncture(EA)underlying preventing AHL.Methods Thirty 3-month-old guinea pigs were randomly divided into control group

model group and EA group(n=10in each group)

and ten 18-month-old guinea pigs were allocated as elderly group.The AHL model was established by subcutaneous injection of D-galactose.EA was applied to bilateral"Yifeng"(SJ 17)and"Tinggong"(SI 19)for 15 min in the EA group while modeling

once daily for 6weeks.After treatment

the latency of auditory brainstem response(ABR)Ⅲ wave was measured by a brain-stem evoked potentiometer.The expressions of COMT in the inferior colliculus and auditory cortex were detected by Western blot.Results Compared with the control group

the latencies of ABRⅢwave were significantly prolonged and the expressions of COMT in the inferior colliculus and auditory cortex were significantly decreased in the model group and the elderly group(P<0.05).After the treatment

the latency of ABRⅢ wave was significantly shortened and the expressions of COMT in the inferior colliculus and auditory cortex were significantly increased in the EA group in comparison with the model group(P<0.05).Conclusion EA at"Yifeng"(SJ 17)and "Tinggong"(SI 19)can improve the hearing of age-related deafness in guinea pigs

which may contribute to its effect in up-regulating the expression of COMT in the inferior colliculus and auditory cortex.

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