Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats

LIU Li; WU Xue-fen; ZHENG Xue-na; GUO Xin; YUE Zeng-hui; LIU Mi; PENG Yan; YUAN Jian-ling

doi:10.13702/j.1000-0607.2017.03.009

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Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats

更新时间：2023-08-11

- Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats
- Acupuncture Research Vol. 42, Issue 3, Pages: 240-245(2017)
- 作者机构：
  
  湖南中医药大学针灸推拿学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2017.03.009
  CLC：
- Published：2017
- 稿件说明：
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LIU Li, WU Xue-fen, ZHENG Xue-na, et al. Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats[J]. Acupuncture research, 2017, 42(3): 240-245.
DOI：

LIU Li, WU Xue-fen, ZHENG Xue-na, et al. Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats[J]. Acupuncture research, 2017, 42(3): 240-245. DOI： 10.13702/j.1000-0607.2017.03.009.

摘要

目的:观察点灸、电针对糖尿病胃轻瘫(DGP)大鼠胃肠运动和内皮细胞型一氧化氮合酶(eNOS)、血管紧张素Ⅱ(ATⅡ)mRNA表达的影响

探讨点灸、电针治疗DGP的效应差异及作用机制。方法:SD大鼠随机分为空白组、模型组、点灸组、电针组

每组10只。采用腹腔注射2%链脲佐菌素配合高脂高糖饮食建立DGP大鼠模型。点灸组和电针组选取"足三里""三阴交""梁门"穴

点灸组于0、10、20min对穴位进行点灸

电针组电针20min

每日治疗1次

治疗15d。检测各组大鼠血糖、胃排空率和小肠推进率

ELISA法检测血浆内皮素1(ET-1)含量

Real-time PCR法检测胃窦部组织eNOS mRNA、ATⅡmRNA的表达。结果:与空白组比较

模型组血糖明显升高(P<0.01);与模型组比较

点灸组和电针组血糖明显降低(P<0.05)。与空白组比较

模型组胃排空率及小肠推进率显著降低(P<0.01)

血浆ET-1含量显著升高(P<0.01)

胃窦部eNOS mRNA表达显著降低、ATⅡmRNA表达显著升高(P<0.01)。与模型组比较

点灸组与电针组胃排空率及小肠推进率显著升高

血浆ET-1含量显著降低

胃窦部eNOS mRNA表达显著升高、ATⅡmRNA表达显著降低(P<0.05)。点灸组与电针组之间比较差异无统计学意义(P>0.05)。结论:点灸与电针均可有效促进DGP大鼠胃肠运动

改善胃排空迟缓症状

两种疗法疗效相当

其作用机制可能与升高胃窦部eNOS mRNA表达、降低ATⅡmRNA表达有关。

Abstract

Objective To observe the effect of point-moxibustion and electroacupuncture(EA)on gastrointestinal motility

and expression of endothelial nitric oxide synthase(eNOS)mRNA and angiotensin 2(ATⅡ)mRNA in gastric antrum in diabetic gastroparesis(DGP)rats

so as to reveal the different effect between point-moxibustion and EA

and explore the mechanism underlying improvement of DGP.Methods Forty SD rats were randomly divided into normal

model

point-moxibustion and EA groups

10 rats in each group.The DGP model was established by intraperitoneal injection of streptozotocin(STZ

55mmol/kg)and fed with high-sugar and high-fat fodder."Zusanli"(ST 36)

"Sanyinjiao"(SP 6)and"Liangmen"(ST 21)were chosen to be stimulated with moxibustion at 0

10 and 20min in the point-moxibustion group or with EA for 20 min in the EA group.The treatment was conducted once daily for 15 days.After treatment

blood glucose

gastric emptying rate and small intestinal propulsive rate were measured.The plasma endothelin 1(ET-1)content was detected by ELISA.The mRNA expression of eNOS and AT Ⅱin gastric antrum were detected by Real-time PCR.Results Compared with the normal group

the blood glucose in the model group was significantly higher(P<0.01).Compared with the model group

the blood glucose decreased significantly in the point-moxibustion group and EA group(P<0.05).In comparison with the normal group

gastric emptying rate and small intestinal propulsive rate were decreased

the content of plasma ET-1was increased

the expression of eNOS mRNA in gastric antrum was significantly reduced and the expression of ATⅡ mRNA was significantly increased in the model group(P<0.01).In comparison with the model group

gastric emptying rate and small intestinal propulsive rate were increased

the content of plasma ET-1was decreased

the expression of eNOS mRNA in gastric antrum was higher and the expression of AT Ⅱ mRNA was significantly lower in both point-moxibustion group and EA group(P<0.05)

and there were no significant differences between the two treatment groups in above mentioned indexes(P>0.05).Conclusion Both point-moxibustion and EA can effectively promote gastrointestinal motility in DGP rats and improve the symptoms of delayed gastric emptying.The two therapies are effective

and their mechanism may be related to the increase of eNOS mRNA expression and the decrease of ATⅡmRNA expression in gastric antrum.

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