Effects of Electroacupuncture on Apoptosis-related Protein Expression of Gastrocnemius Muscle Cells in Rats with Denervated Sciatic Nerve

GAO Rui-qi; TANG Cheng-lin; HUANG Si-qin; CAO Jing; GUO Quan-hu; ZHANG Yi; TIAN Yuan; YUAN Hai-zhou; ZHAO Dan-dan; LUO Ao; ZHANG An-ning

doi:10.13702/j.1000-0607.2017.04.004

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Effects of Electroacupuncture on Apoptosis-related Protein Expression of Gastrocnemius Muscle Cells in Rats with Denervated Sciatic Nerve

更新时间：2023-08-11

- Effects of Electroacupuncture on Apoptosis-related Protein Expression of Gastrocnemius Muscle Cells in Rats with Denervated Sciatic Nerve
- Acupuncture Research Vol. 42, Issue 4, Pages: 302-307(2017)
- 作者机构：
  
  重庆医科大学中医药学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2017.04.004
  CLC：
- Published：2017
- 稿件说明：
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GAO Rui-qi, TANG Cheng-lin, HUANG Si-qin, et al. Effects of Electroacupuncture on Apoptosis-related Protein Expression of Gastrocnemius Muscle Cells in Rats with Denervated Sciatic Nerve[J]. Acupuncture research, 2017, 42(4): 302-307.
DOI：

GAO Rui-qi, TANG Cheng-lin, HUANG Si-qin, et al. Effects of Electroacupuncture on Apoptosis-related Protein Expression of Gastrocnemius Muscle Cells in Rats with Denervated Sciatic Nerve[J]. Acupuncture research, 2017, 42(4): 302-307. DOI： 10.13702/j.1000-0607.2017.04.004.

摘要

目的:观察电针疗法对失坐骨神经大鼠腓肠肌细胞凋亡及相关蛋白的影响

探讨电针对失坐骨神经骨骼肌萎缩的延缓效应及其可能机制。方法:SD大鼠随机分为假手术组、模型组和电针组

每组21只。采用切断坐骨神经法制备腓肠肌萎缩大鼠模型。电针组电针患侧"足三里""承山"穴

每日1次

每次10min

各组分别在术后7、14、21d取材。TUNEL法检测腓肠肌细胞凋亡

Western blot法检测Bcl-2、Bcl-2相关的X蛋白(Bax)、细胞色素C(Cyt-C)以及天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)的蛋白表达

RT-PCR法检测腓肠肌Caspase-3mRNA表达。结果:模型组各时点细胞凋亡指数均高于假手术组(P<0.05)

电针组的细胞凋亡指数显著低于模型组(P<0.05)。模型组各时点腓肠肌Bcl-2蛋白表达较假手术组显著降低(P<0.05)

Bax、Cyt-C以及Caspase-3蛋白表达均明显升高(P<0.05)。术后14、21d

电针组与模型组比较

Bcl-2蛋白表达显著升高(P<0.05)

Bax、Cyt-C及Caspase-3蛋白表达则明显降低(P<0.05)。各组大鼠腓肠肌的Caspase-3mRNA表达与蛋白表达结果基本一致。结论:电针能够促进腓肠肌Bcl-2的表达并抑制Bax、Cyt-C和Caspase-3的表达

从而抑制肌细胞的凋亡

这可能是电针延缓失神经性骨骼肌萎缩的机制之一。

Abstract

Objective To observe the anti-apoptosis effect of electroacupuncture(EA)on gastrocnemius muscle cells in rats with denervated sciatic nerve

so as to explore its possible mechanisms underlying delaying atrophy of skeletal muscle.Methods Sixty-three male Sprague-Dawley rats were randomly divided into sham operation group

model group

and EA group

and then further divided into 3subgroups in each group(n=7/subgroup).Gastrocnemius muscle atrophy model was established by transecting the sciatic nerve of rat.EA(5Hz

1.5mA)was applied to "Zusanli"(ST 36)and "Chengshan"(BL 57)acupoints at the affected side for 10 min

once a day for 1

3 weeks

respectively.The gastrocnemius muscles were sampled on the 7

(th)

14 (th)

(th)

21(th)

(th)

d after modeling

separately.The apoptotic cells were detected by TUNEL staining.Bcl-2

Bax

Cyt-C and Caspase-3 protein expressions were determined by Western blot.RT-PCR was used to check Caspase-3 gene expression.Results Compared with the sham operation group

the cell apoptotic index in gastrocnemius was markedly higher

gastrocnemius Bcl-2 protein expression was markedly down-regulated

and gastrocnemius Bax

Cyt-C and Caspase-3 protein expressions were considerably up-regulated in the model group at all time-points(P

0.05).Compared with the model group

the cell apoptotic indexes in gastrocnemius were significantly lower in the three EA subgroups(P

0.05).Bcl-2 protein expressions were markedly up-regulated while Bax

Cyt-C and Caspase-3 protein expressions were significantly down-regulated in the EA group 14

21 days after modeling compared with the corresponding model subgroups(P

0.05).The changes of Caspase-3 mRNA expression levels of gastrocnemius in all groups were similar to those of Caspase-3 protein expressions.Conclusion Electroacupuncture intervention can effectively increase Bcl-2 expression and decrease Bax

Cyt-C and Caspase-3 expression in gastrocnemius muscle

and consequently reduce the apoptosis of muscle cell

which may contribute to its effect in delaying the skeletal muscle atrophy of denervated sciatic nerve.

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