Effects of Chiropractics on Intervertebral Disk Extracell Matrix and Metabolic Enzymes in Rats with Cervical Spondylosis

HUANG Li-shan; HUANG Ping-ping; TONG Xiu-bing; LI Zhong-li; FANG Yan-ping; LIAO Jun

doi:10.13702/j.1000-0607.2017.04.007

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Effects of Chiropractics on Intervertebral Disk Extracell Matrix and Metabolic Enzymes in Rats with Cervical Spondylosis

更新时间：2023-08-11

- Effects of Chiropractics on Intervertebral Disk Extracell Matrix and Metabolic Enzymes in Rats with Cervical Spondylosis
- Acupuncture Research Vol. 42, Issue 4, Pages: 321-326(2017)
- 作者机构：
  
  1. 福建中医药大学针灸学院
  2. 中国中医科学院针灸研究所
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2017.04.007
  CLC：
- Published：2017
- 稿件说明：
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HUANG Li-shan, HUANG Ping-ping, TONG Xiu-bing, et al. Effects of Chiropractics on Intervertebral Disk Extracell Matrix and Metabolic Enzymes in Rats with Cervical Spondylosis[J]. Acupuncture research, 2017, 42(4): 321-326.
DOI：

HUANG Li-shan, HUANG Ping-ping, TONG Xiu-bing, et al. Effects of Chiropractics on Intervertebral Disk Extracell Matrix and Metabolic Enzymes in Rats with Cervical Spondylosis[J]. Acupuncture research, 2017, 42(4): 321-326. DOI： 10.13702/j.1000-0607.2017.04.007.

摘要

目的:观察捏脊对颈椎病大鼠椎间盘基质金属蛋白酶(MMP)-1、MMP-3、MMP-13、细胞内Ⅱ型胶原(COL-Ⅱ)、基质金属蛋白酶抑制因子1(TIMP-1)以及细胞外基质(ECM)的影响

探讨捏脊治疗颈椎病的可能机制。方法:SD大鼠随机分为假手术组、模型组、捏脊组、莫比可组

每组8只。采用动静力失衡法建立颈椎病大鼠模型。捏脊组予捏脊干预治疗

每天14次;莫比可组予莫比可0.78mg/kg灌胃干预

每日1次

14d为1个疗程

共2个疗程。免疫组化法检测大鼠椎间盘组织中MMP-1、MMP-3、MMP-13、COL-Ⅱ、TIMP-1的表达量

免疫印迹法检测ECM的蛋白表达水平。结果:与假手术组比较

模型组大鼠椎间盘组织中MMP-1、MMP-3、MMP-13表达量均升高(P<0.05)

COL-Ⅱ、TIMP-1表达均降低(P<0.05);与模型组比较

捏脊组与莫比可组大鼠椎间盘组织中MMP-1、MMP-3、MMP-13表达均降低(P<0.05)

COL-Ⅱ、TIMP-1表达均升高(P<0.05)。与假手术组比较

模型组大鼠椎间盘组织中ECM蛋白表达降低(P<0.05);与模型组比较

捏脊组与莫比可组ECM蛋白表达升高(P<0.05)。结论:捏脊治疗颈椎病大鼠椎间盘退变可能是通过调节椎间盘ECM系统及相关代谢酶表达量实现的。

Abstract

Objective To observe the effect of chiropractics on intervertebral disk extracell matrix metalloproteinase(MMP)-1

MMP-3

MMP-13

cellular collagen typeⅡ(COL-Ⅱ)

tissue inhibitor 1 of metalloproteinase(TIMP-1)and extracellular matrix(ECM)in the rats with cervical spondylosis

so as to explore its mechanism underlying improvement of cervical spondylosis.Methods A total of 40 SD rats were randomized into a sham group

a model group

a chiropractics group and a mobic group

8rats in each one.The model was established by static-dynamic imbalance method.The rats in the chiropractics group were treated with chiropractic therapy for two courses

14 times a day and 14 days as a course.The rats in the mobic group were treated with intragastric administration of mobic.The expression of MMP-1

MMP-3

MMP-13

COL-Ⅱ and TIMP-1 in the intervertebral disk tissue were detected by immunohistochemistry

and the protein expression of ECM was detected by Western blot.Results Compared with those in the sham group

the expressions of MMP-1

MMP-3

MMP-13 in the model group increased(all P<0.05)

and COL-Ⅱ

TIMP-1 decreased(both P<0.05).Compared with those in the model group

the expressions of MMP-1

MMP-3

MMP-13 in the chiropractics group and the mobic group decreased(all P <0.05)

and COL-Ⅱ

TIMP-1 increased(all P<0.05).Compared with that in the sham group

the expression of ECM protein in the model group decreased(P<0.05).Compared with that in the model group

the expression of ECM protein in the chiropractics group and the mobic group increased(both P<0.05).Conclusion Chiropractics may improve the degeneration in rats with cervical spondylosis by regulating intervertebral disc ECM system and relevant metabolic enzymes.

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