Effect of Electroacupuncture on Expression of 5-HT_7 Receptor in Periaqueductal Gray and Plasma Calcitonin Gene-related Peptide in Migraine Rats

PEI Pei; CHEN Huai-zhen; WANG Yan-xin; YANG Wen-ming; LIU Lu; WANG Lin-peng

doi:10.13702/j.1000-0607.2017.06.008

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Effect of Electroacupuncture on Expression of 5-HT_7 Receptor in Periaqueductal Gray and Plasma Calcitonin Gene-related Peptide in Migraine Rats

更新时间：2023-08-11

- Effect of Electroacupuncture on Expression of 5-HT_7 Receptor in Periaqueductal Gray and Plasma Calcitonin Gene-related Peptide in Migraine Rats
- Acupuncture Research Vol. 42, Issue 6, Pages: 510-513(2017)
- 作者机构：
  
  1. 安徽中医药大学第一附属医院脑病中心
  2. 首都医科大学附属北京中医医院针灸中心
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2017.06.008
  CLC：
- Published：2017
- 稿件说明：
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PEI Pei, CHEN Huai-zhen, WANG Yan-xin, et al. Effect of Electroacupuncture on Expression of 5-HT_7 Receptor in Periaqueductal Gray and Plasma Calcitonin Gene-related Peptide in Migraine Rats[J]. Acupuncture research, 2017, 42(6): 510-513.
DOI：

PEI Pei, CHEN Huai-zhen, WANG Yan-xin, et al. Effect of Electroacupuncture on Expression of 5-HT_7 Receptor in Periaqueductal Gray and Plasma Calcitonin Gene-related Peptide in Migraine Rats[J]. Acupuncture research, 2017, 42(6): 510-513. DOI： 10.13702/j.1000-0607.2017.06.008.

摘要

目的:探讨电针是否能通过调控中脑导水管周围灰质(PAG)5-羟色胺7(5-HT7)受体和外周血降钙素基因相关肽(CGRP)介导抗偏头痛。方法:雄性SD大鼠随机分为对照组、模型组和电针组

每组14只。反复电刺激大鼠上矢状窦区硬脑膜诱发神经源性偏头痛模型。对照组仅予颅内电极安置

电针组于硬脑膜电刺激后电针"风池"穴

每日1次

治疗6次。采用免疫荧光标记法和Western blot法检测PAG区5-HT7受体的表达

放射免疫法检测外周血CGRP含量。结果:与对照组比较

反复硬脑膜电刺激造模后PAG区5-HT7受体阳性神经元数目和蛋白相对表达量及外周血CGRP含量显著增高(均P<0.001);与模型组比较

电针组5-HT7阳性神经元数目和蛋白相对表达量显著降低(P<0.01)

外周血CGRP含量降低(P<0.05)。结论:电针"风池"穴可下调偏头痛大鼠PAG区5-HT7受体表达

降低其外周血CGRP含量。

Abstract

Objective To explore the analgesic effect of electroacupuncture(EA)by modulating 5-hydroxytryptamine 7(5-HT7)receptor in periaqueductal gray(PAG)and plasma calcitonin gene-related peptide(CGRP).Methods Forty-two male Sprague Dawley(SD)rats were randomly divided into control

model and EA groups

14 rat in each one.The neurogenic migraine model was established by repeated electrical stimulation on sagittal sinus duramater.Intracranial electrodes were used in the control group without stimuli.The rats in the EA group received EA(0.5-1 mA

2 Hz/15 Hz)at"Fengchi"(GB 20)for 10 min after dural electrical stimulation

once a day for 6 days.The expression of 5-HT7 receptor in the PAG was assessed by immunofluorescence and Western blot

respectively;plasma CGRP was measured by radioimmunoassay.Results Compared with the control group

the positive neuron number and protein expression of 5-HT7 receptor in PAG and plasma CGRP increased after model establishment(all P<0.001).The above mentioned indexes were reversed in the EA group compared with those in the model group(the positive neuron number and protein expression of 5-HT7 receptor

P<0.01;plasma CGRP

P<0.05).Conclusion EA at GB 20 can down-regulate the expression of 5-HT7 receptor in the PAG and reduce the content of plasma CGRP in the rats of migraine.

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Related Author

PEI Pei

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Related Institution

Encephalopathy Center, the First Affiliated Hospital, Anhui University of Chinese Medicine

Graduate School, Anhui University of Chinese Medicine

Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University

College of Integrated Traditional Chinese and Western Medicine, Southwest Medical University

The Second Affiliated Hospital, Guilin Medical University

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