QIN Mei-xiang, SU Sheng-yong, ZHANG Xi, et al. Effect of mild moxibustion on pain and expression of LC3 and Bax in spinal cord in cervical spondylotic radiculopathy rats[J]. Acupuncture research, 2022, 47(3): 244-249.
DOI:
QIN Mei-xiang, SU Sheng-yong, ZHANG Xi, et al. Effect of mild moxibustion on pain and expression of LC3 and Bax in spinal cord in cervical spondylotic radiculopathy rats[J]. Acupuncture research, 2022, 47(3): 244-249. DOI: 10.13702/j.1000-0607.20210045.
Effect of mild moxibustion on pain and expression of LC3 and Bax in spinal cord in cervical spondylotic radiculopathy rats
目的:观察温和灸“大椎”对神经根型颈椎病(CSR)大鼠脊髓组织自噬与凋亡因子LC3、Bax蛋白及mRNA的影响,探讨运用温和灸治疗CSR的镇痛作用机制。方法:SD大鼠随机分为空白组、模型组、温和灸组、温和灸+自噬抑制剂3-甲基腺嘌呤(3-MA)组,每组10只。采用渔线压迫颈局部神经根法制备CSR大鼠模型。于造模3 d后,温和灸组取“大椎”进行温和灸10 min治疗并予腹腔注射1 mL 0.9%氯化钠溶液,温和灸+3-MA组予温和灸“大椎”和腹腔注射1 mL 3-MA
模型组仅予腹腔注射1 mL 0.9%氯化钠溶液,均每日1次,连续干预7 d。治疗后进行大鼠步态障碍评分,并采用YLS-3E型痛分析仪测量机械痛阈,免疫组化法检测脊髓组织LC3、Bax的蛋白表达,RT-PCR法检测脊髓组织LC3、Bax mRNA表达水平。结果:与空白组比较,模型组步态障碍评分明显升高(P<0.01)
Objective To observe the effect of mild moxibustion(Moxi) at “Dazhui”(GV14) on neuropathic pain
expression of autophagy and apoptosis factor LC3 and Bax proteins and mRNAs in the spinal cord tissue in rats with cervical spondylotic radiculopathy(CSR)
so as to explore its underlying mechanism underlying relief of CSR-induced pain. Methods Forty rats(half male half female) were randomly divided into blank control
model
Moxi
Moxi+autophagy inhibitor 3-methyladenine(3-MA
Moxi+3-MA) groups
with 10 rats in each group. The CSR model was established by loose ligature of the local cervical nerve roots. Three days after modeling
mild Moxi was applied to GV14 for 10 min
once daily for 7 days. Rats of the Moxi+3-MA group received intraperitoneal injection of 3-MA(1 mL
15 mg/kg+ saline) before Moxi
once daily for 7 consecutive days. Rats of the model and Moxi groups were also given normal saline(i.p.
1 mL)
once daily for 7 days. The gait behavior score(1-3 points) was scaled according to the rats' pain reaction and foot paw contracture produced walking disorder and the mechanical pain threshold(MPT) was detected before and after the treatment. The expression of spinal cord LC3 and Bax proteins and mRNAs were detected by immunohistochemistry and quantitative RT-PCR
respectively. Results Compared with the blank control group
the gait disorder score
and percentage of Bax positive cells and expression of Bax mRNA were significantly increased(P<0.01
P<0.05)
and MPT was markedly decreased in the model group(P<0.01). After the treatment
the gait disorder score
percentage of Bax positive cells and Bax mRNA expression were significantly down-regulated(P<0.01
P<0.05)
while the MPT and percentage of LC3 positive cells and LC3 mRNA expression were considerably increased(P<0.01
P<0.05) in both Moxi and Moxi+3-MA groups. The therapeutic effects of mild Moxi were remarkably superior to those of Moxi+3-MA in downregulating gait disorder score
Bax positive cell percentage and Bax mRNA expression
and in up-regulating MPT
LC3 positive cell percentage and LC3 mRNA expression(P<0.05)
suggesting a reduction of the function of mild Moxi after administration of 3-MA. Conclusion Mild Moxi at GV14 can relieve neuropathic pain in CSR rats
which may be related to its functions in up-regulating LC3 autophagy
thereby inhibiting the expression of Bax pro-apoptotic protein in spinal cord to reduce apoptosis and to repair nerve injury.
Effects of mild moxibustion on Beclin-1/Bcl-2 expression in spinal cord of rats with cervical spondylotic radiculopathy
Effect of mild moxibustion at Jiuwei (CV15) on the diaphragm and postural control ability of patients after recovery from stroke complicated with pulmonary infection: a randomized controlled trial
Effects of mild moxibustion on visceral hypersensitivity via SCF/c-kit signaling pathway in diarrhea-predominant irritable bowel syndrome rats with syndrome of liver-qi stagnation and spleen deficiency
Related Author
CAI Hui-qian
SU Sheng-yong
ZHANG Xi
QIN Mei-xiang
HUANG Xiao-zhen
DAI Qi
JIANG Xiang-yu
LIN An
Related Institution
Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Guangxi University of Chinese Medicine
The First Clinical Medical College, Guangxi University of Chinese Medicine
Department of Science Education and Information, Quanzhou Hospital of Traditional Chinese Medicine, Quanzhou 362000, Fujian Province, China), (, Quanzhou Hospital of Traditional Chinese Medicine
Department of Acupuncture, (, Quanzhou Hospital of Traditional Chinese Medicine, , , )
Department of Acupuncture and Rehabilitation, (, Quanzhou Hospital of Traditional Chinese Medicine, , , )