Effect of moxibustion on inflammatory pain and N-methyl-D aspartic acid receptor-nitric oxide-cyclic GMP pathway in spinal cord of adjuvant arthritis rats
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Effect of moxibustion on inflammatory pain and N-methyl-D aspartic acid receptor-nitric oxide-cyclic GMP pathway in spinal cord of adjuvant arthritis rats
PENG Chuan-yu, HU Ling, WU Zi-jian, et al. Effect of moxibustion on inflammatory pain and N-methyl-D aspartic acid receptor-nitric oxide-cyclic GMP pathway in spinal cord of adjuvant arthritis rats[J]. Acupuncture research, 2022, 47(3): 250-255.
DOI:
PENG Chuan-yu, HU Ling, WU Zi-jian, et al. Effect of moxibustion on inflammatory pain and N-methyl-D aspartic acid receptor-nitric oxide-cyclic GMP pathway in spinal cord of adjuvant arthritis rats[J]. Acupuncture research, 2022, 47(3): 250-255. DOI: 10.13702/j.1000-0607.20210557.
Effect of moxibustion on inflammatory pain and N-methyl-D aspartic acid receptor-nitric oxide-cyclic GMP pathway in spinal cord of adjuvant arthritis rats
0.01)。结论:艾灸可改善AA大鼠的炎性痛反应,其作用机制可能与下调脊髓组织中NMDA-NO-cGMP通路的功能有关。Objective To observe the effect of moxibustion on pain and N-methyl-D aspartic acid receptor/nitric oxide/cyclic guanosine monophosphate(NMDA-NO-cGMP) signaling pathway in the spinal cord of rats with adjuvant arthritis(AA)
so as to explore its underlying mechanisms in relieving inflammatory pain of rheumatoid arthritis(RA). Methods SD rats were randomly divided into normal
model
moxibustion(Moxi)
Moxi +NMDA receptor antagonist AP-5(Moxi+AP-5) and Moxi +NMDA receptor agonist(NMDA) groups
with 20 rats in each group. The AA model was established by placing the rats in a wind
cold and damp environment for 12 h
once daily for 20 days and by injection of complete Freund's adjuvant into the right hind paw. Rats of the three Moxi groups received ignited moxa-stick stimulation of “Zusanli”(ST36) and “Shenshu”(BL23) alternately for 20 min
once a day for 15 days. The Moxi + AP-5 group and Moxi +NMDA group received intraperitoneal injection of AP-5(0.7 mg·kg(-1))后进行与艾灸组相同的干预;3组均每日1次,共干预15 d。造模前后及干预后测定大鼠右后足底机械痛阈(PWT);荧光定量PCR法和Western blot法检测脊髓组织中诱导型一氧化氮合酶(iNOS) mRNA和蛋白的表达;ELISA法检测脊髓组织中cGMP的含量;比色法和硝酸还原酶法分别检测脊髓组织中NOS的活性和NO的含量。结果:造模后,与正常组比较,其余各组右后足底PWT均显著降低(P
Objective To observe the effect of moxibustion on pain and N-methyl-D aspartic acid receptor/nitric oxide/cyclic guanosine monophosphate(NMDA-NO-cGMP) signaling pathway in the spinal cord of rats with adjuvant arthritis(AA)
so as to explore its underlying mechanisms in relieving inflammatory pain of rheumatoid arthritis(RA). Methods SD rats were randomly divided into normal
model
moxibustion(Moxi)
Moxi +NMDA receptor antagonist AP-5(Moxi+AP-5) and Moxi +NMDA receptor agonist(NMDA) groups
with 20 rats in each group. The AA model was established by placing the rats in a wind
cold and damp environment for 12 h
once daily for 20 days and by injection of complete Freund's adjuvant into the right hind paw. Rats of the three Moxi groups received ignited moxa-stick stimulation of “Zusanli”(ST36) and “Shenshu”(BL23) alternately for 20 min
once a day for 15 days. The Moxi + AP-5 group and Moxi +NMDA group received intraperitoneal injection of AP-5(0.7 mg·kg
(-1)
·d(-1)·d
(-1)
) and NMDA(5 mg·kg(-1)) and NMDA(5 mg·kg
(-1)
·d(-1)·d
(-1)
)
respectively
once a day
for a total of 15 days. Mechanical pain thres-hold(MPT) was measured before and after modeling and interventions. The spinal cord tissue was sampled for detecting the expression of iNOS mRNA and protein
content of cGMP and NO
and the activity of NOS by using fluorescence quantitative PCR
Western blot
ELISA
nitrate reductase method and colorimetric method
respectively. Results Before modeling
there was no significant difference in MPT among all the 5 groups(P
>
0.05). After modeling
the MPT was remarkably decreased(P
<
0.01)
the expression levels of iNOS mRNA and protein
the contents of cGMP and NO
the activity of NOS were significantly increased in the model group relevant to the normal group(P
<
0.01). After the interventions
the MPT was obviously increased(P
<
0.01)
while the expression levels of iNOS mRNA and protein
the contents of cGMP and NO
the activity of NOS were significantly down-regulated in the Moxi
Moxi-AP-5 and Moxi+NMDA groups(P
<
0.05
P
<
0.01). The effect of Moxi+AP-5 group was significantly superior to that of Moxi group in raising MPT and down-regulating the expression levels of iNOS mRNA and protein
and the content of NO(P
<
0.05
P
<
0.01). The effect of Moxi+NMDA group was obviously inferior to that of Moxi group in up-regulating MPT and down-regulating the levels of iNOS mRNA and protein
and the contents of cGMP and NO
and the activity of NOS(P
<
0.01)
suggesting a reduction of the therapeutic effects in raising MPT and down-regulating expression of iNOS mRNA and protein after administration of AP-5. Conclusion Moxibustion can relieve RA inflammatory pain in AA rats
which may be related to its function in down-regulating the NMDA/NO/cGMP signaling pathway in the spinal cord.
Study on the mechanism of moxibustion regulating ferroptosis-lipid metabolism pathway to improve synovitis inflammatory injury in rheumatoid arthritis rats
Effect of moxibustion on the expressions of hypoxia inducible factor-1α and vascular endothelial growth factor in ankle synovial tissue of rats with adjuvant arthritis
Effect of moxibustion on the indicators of autophagy and apoptosis in synovium of rats with adjuvant arthritis
Related Author
PENG Chuan-yu
WANG Tian-cheng
CAI Rong-lin
HAO Feng
HE Lu
LIU Lei
WANG Jie
ZHONG Yu-xiu
Related Institution
School of Acupuncture-Moxibustion and Tuina, Anhui University of Chinese Medicine
School of lntegrated Traditional and Western Medicine, Anhui University of Chinese Medicine
Institute of Acupuncture and Meridian, Anhui Academy of Traditional Chinese Medicine
School of Acupuncture-Moxibustion and Tuina, Nanjing University of Chinese Medicine