Electroacupuncture ameliorates ischemic injury in cerebral ischemia-reperfusion rats by regulating endogenous melatonin and inhibiting the activation of astrocytes
|更新时间:2023-08-11
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Electroacupuncture ameliorates ischemic injury in cerebral ischemia-reperfusion rats by regulating endogenous melatonin and inhibiting the activation of astrocytes
ZHONG Xiao-yong, RUAN Su, WANG Fang, et al. Electroacupuncture ameliorates ischemic injury in cerebral ischemia-reperfusion rats by regulating endogenous melatonin and inhibiting the activation of astrocytes[J]. Acupuncture research, 2022, 47(1): 39-45.
DOI:
ZHONG Xiao-yong, RUAN Su, WANG Fang, et al. Electroacupuncture ameliorates ischemic injury in cerebral ischemia-reperfusion rats by regulating endogenous melatonin and inhibiting the activation of astrocytes[J]. Acupuncture research, 2022, 47(1): 39-45. DOI: 10.13702/j.1000-0607.20210738.
Electroacupuncture ameliorates ischemic injury in cerebral ischemia-reperfusion rats by regulating endogenous melatonin and inhibiting the activation of astrocytes
Objective To observe the effect of electroacupuncture(EA)at “Baihui”(GV20) and “Shenting”(GV24) on the expression of melatonin synthesis rate-limiting enzyme―arylalkylamine N-acetyltransferase(AANAT)in pineal gland of rats with focal cerebral ischemia-reperfusion injury
so as to explore the mechanism of EA underlying improving ischemia-reperfusion injury.Methods Forty-eight SD rats were randomly divided into sham operation
model
EA and non-acupoint groups
with 12 rats in each group. The focal cerebral ischemia-reperfusion injury rat model was established by occlusion of the middle cerebral artery. Rats of the EA group received EA at GV20 and GV24
while those in the non-acupoint group received EA at non-acupoints below the costal margins on both sides for 20 min
once daily for 7 days. The neurological deficit score(0 to 4 points) was given after successful modeling according to Longa's method. Morris water maze test was used to assess the cognitive function of rat. ELISA was used to detect the plasma melatonin content
and PCR and Western blot were used to detect the mRNA and protein expressions of AANAT in the pineal gland
separately. Immunofluorescence staining was used to detect the activation of astrocytes and neuronal injury in the hippocampus.Results After focal cerebral ischemia-reperfusion injury and compared with the sham operation group
the neurological deficit score
the escape latency
and the expression of GFAP were significantly increased(P<0.01)
while the times of platform quadrant crossing
the secretion of melatonin at 24:00
AANAT mRNA and protein expression levels and NeuN protein expression were significantly down-regulated(P<0.01). After EA at GV20 and GV24
the above-mentioned indexes all reversed in the EA group relative to the model group
and there were significant differences between the two groups(P<0.01). Compared with the model group
the changes of the abovementioned indexes in the non-acupoint group were not statistically significant(P>0.05).Conclusion EA at GV20 and GV24 can alleviate neurological deficit and improve cognitive function in cerebral ischemia-reperfusion rats
which may be related to its effects in up-regulating endogenous melatonin levels
inhibiting the activation of astrocytes and protecting damaged neurons in the hippocampus.
Mechanism of analgesic effect of electroacupuncture on rats with cervical spondylosis radiculopathy based on activation of astrocytes and HMGB1/TLR4/MyD88 signaling pathway
Electroacupuncture alleviates neurological function via activating the Yap-OPA1 axis and mitochondrial fusion in rats with cerebral ischemia-reperfusion injury
Electroacupuncture improves learning and memory impairment and enhances hippocampal synaptic plasticity through BDNF/TRKB/CREB signaling pathway in cerebral ischemia-reperfusion injury rats
Related Author
GUO Yan-jun
SU Sheng-yong
SU Hong
YANG Pu
LI Jing
XIE Cai-yun
LIANG Hui
RUAN Su
Related Institution
Guangxi University of Chinese Medicine
Guangxi Key Laboratory of Molecular Biology of Traditional Chinese Medicine
The First Affiliated Hospital of Guangxi University of Chinese Medicine
Neurology Department, (, The Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, , )
Department of Rehabilitation, (, The Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, , )