Effect of electroacupuncture on gut microbiota and serum IL-1β and IL-18 in rats with vascular dementia based on principle of “curing brain disorders by treating intestines”
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Effect of electroacupuncture on gut microbiota and serum IL-1β and IL-18 in rats with vascular dementia based on principle of “curing brain disorders by treating intestines”
CHEN Dan-feng, ZHANG Hong, XIE Ju-ying, et al. Effect of electroacupuncture on gut microbiota and serum IL-1β and IL-18 in rats with vascular dementia based on principle of “curing brain disorders by treating intestines”[J]. Acupuncture research, 2022, 47(3): 216-223.
DOI:
CHEN Dan-feng, ZHANG Hong, XIE Ju-ying, et al. Effect of electroacupuncture on gut microbiota and serum IL-1β and IL-18 in rats with vascular dementia based on principle of “curing brain disorders by treating intestines”[J]. Acupuncture research, 2022, 47(3): 216-223. DOI: 10.13702/j.1000-0607.20210766.
Effect of electroacupuncture on gut microbiota and serum IL-1β and IL-18 in rats with vascular dementia based on principle of “curing brain disorders by treating intestines”
Objective To observe the effect of electroacupuncture(EA) of “Baihui”(GV20)
“Dazhui”(GV14)
“Shenshu”(BL23)and “Zusanli”(ST36) on the intestinal flora and serum interleukin(IL)-1β and IL-18 contents in vascular dementia(VD) rats. Methods SD rats were randomized into sham operation
VD model
GV20+GV14+BL23(EA-basic acupoints)
and EA-basic acupoints+ST36 and EA-basic acupoints+probiotics groups(n=10 in each group). EA(10 Hz/50 Hz) was conducted for 30 min
once daily for 4 consecutive weeks. Rats of the EA-basic acupoints+probiotics received gavage of probiotics(2 mL/d containing 2.0×10
9
CFU of live bifidobacterium)
once a day for 4 weeks
and those of the EA-basic acupoints and EA-basic acupoints+ST36 groups received gavage of the same dose of normal saline. The Morris water maze test was used to evalua-te the rats' lear-ning and memory ability before and after the treatment. The serum IL-1β and IL-18 levels were determined by ELISA
and the histopathological changes of the intestinal mucosa were observed by H.E. staining. The ultrastructural changes of hippocampal neurons were observed by using transmission electron microscopy and 16 S rDNA sequencing technique was used to analyze the composition of intestinal microbiome. Results Compared with the sham operation group
the escape latency
serum levels of IL-1β and IL-18
as well as the relative abundance of harmful bacteria(including Catabacter
obinsoniella and Desulfovibrio) in the intestine were significantly increased(P
<
0.01). In comparison with the model group
the escape latency
serum levels of IL-1β and IL-18 in the three treatment groups
and the relative abundance of harmful bacteria(such as the Catabacter
Robinsoniella and Desulfovibrio) in the EA-basic acupoints+ST36 group were down-regulated obviously(P
<
0.05
P
<
0.01)
and the relative abundance of Clostridiales-unclassified in both EA-basic acupoints+probiotics and EA-basic acupoints+ST36 groups was significantly up-regulated(P
<
0.05). The effects of EA-basic acupoints+ST36 and EA-ba-sic acupoints+probiotics were significantly superior to that of EA-basic acupoints in down-regulating IL-18 content(P
<
0.05). H.E. staining showed atrophy of the whole mucosal layer
loss of goblet cells
destruction of glands
infiltration of a large number of inflammatory cells
and transmission microscope displayed fuzziness of the nucleus membrane boundary
cystic dilation of the rough endoplasmic reticulum with unclear structure swelling of the mitochondria
and disordered arrangement or dissolution of the inner cristae in the model group
which was relatively milder in the EA-basic acupoints+ST36 and EA-basic acupoints+probiotics groups. Conclusion EA of GV20+GV14+BL23+ ST36 can improve the cognitive dysfunction of VD model rats
which may be related to its function in regulating the imbalance of intestinal microbiota
thereby inhibiting the peripheral inflammatory factor.
Effect of electroacupuncture at “Baihui” (GV20) and “Shenting” (GV24) on cognitive impairment and mitochondrial energy metabolism in vascular dementia rats
Electroacupuncture improves cognitive function by modulating hippocampal lactate-mediated HIF-1α signaling pathway and inhibiting inflammation response in vascular dementia rats
Effects of electroacupuncture on the microvascular structure and related protein expression in the hippocampus of vascular dementia rats
Related Author
JIA Xiao-yu
LI Hui-jing
RAO Ting
YOU Yong-mei
LI Juan
GONG Yi-meng
ZHONG Dong-ling
JIANG Yi-jing
Related Institution
School of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine
College of Clinical Medicine, Chengdu University of Traditional Chinese Medicine
Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine
Heilongjiang University of Chinese Medicine
The Fifth Department of Acupuncture, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine