Effect of moxibustion on JAK2/STAT3 signal pathway in synovial tissues of rabbits with adjuvant arthritis under the overexpression of NLRP3 inflammatory bodies

XI Dong-lai; YANG Shen-qiao; WANG Yi; CHEN Jun; LU Xiao-qing; ZHOU Hai-yan; MA Wen-bin; WU Fei; YANG Xin

doi:10.13702/j.1000-0607.20211160

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Effect of moxibustion on JAK2/STAT3 signal pathway in synovial tissues of rabbits with adjuvant arthritis under the overexpression of NLRP3 inflammatory bodies

更新时间：2023-08-10

- Effect of moxibustion on JAK2/STAT3 signal pathway in synovial tissues of rabbits with adjuvant arthritis under the overexpression of NLRP3 inflammatory bodies
- Acupuncture Research Vol. 47, Issue 12, Pages: 1095-1100(2022)
- 作者机构：
  
  1. 成都中医药大学针灸推拿学院
  2. 成都中医药大学养生康复学院
  3. 成都中医药大学外语学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.20211160
  CLC：
- Published：2022
- 稿件说明：
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XI Dong-lai, YANG Shen-qiao, WANG Yi, et al. Effect of moxibustion on JAK2/STAT3 signal pathway in synovial tissues of rabbits with adjuvant arthritis under the overexpression of NLRP3 inflammatory bodies[J]. Acupuncture research, 2022, 47(12): 1095-1100.
DOI：

XI Dong-lai, YANG Shen-qiao, WANG Yi, et al. Effect of moxibustion on JAK2/STAT3 signal pathway in synovial tissues of rabbits with adjuvant arthritis under the overexpression of NLRP3 inflammatory bodies[J]. Acupuncture research, 2022, 47(12): 1095-1100. DOI： 10.13702/j.1000-0607.20211160.

摘要

目的:通过慢病毒载体介导NOD样受体热蛋白结构域相关蛋白3(NLRP3)炎性小体在佐剂性关节炎(AA)家兔中过表达，观察艾灸对AA家兔Janus蛋白酪氨酸激酶2-信号转导和转录激活因子3(JAK2-STAT3)信号通路及滑膜液白细胞介素(IL)-1β、IL-18的影响，探讨艾灸治疗类风湿性关节炎的可能机制。方法:将28只家兔随机分为对照组、模型组、艾灸组、NLRP3过表达组，每组7只。采用弗氏完全佐剂注射法制备AA家兔模型。造模后第3天，将NLRP3过表达慢病毒载体注入NLRP3过表达组家兔双侧后膝关节腔内。造模后第7天，艾灸组、NLRP3过表达组给予艾炷灸双侧“肾俞”“足三里”治疗，5壮/穴，1次/d

6 d为1个疗程，疗程间间隔1 d

共3个疗程。造模后第6天、治疗第6天及治疗结束后测量家兔双后膝关节周长。采用ELISA法检测家兔膝关节滑膜液中IL-1β、IL-18的含量，实时荧光定量PCR法检测家兔膝关节滑膜组织中NLRP3、JAK2、STAT3 mRNA表达。结果:与对照组比较，模型组家兔左、右膝关节周长及膝关节滑膜液中IL-1β、IL-18含量增加(P<0.05)

滑膜组织中NLRP3、JAK2、STAT3 mRNA表达均升高(P<0.05);与模型组比较，艾灸组左、右膝关节周长缩小(P<0.05)

膝关节滑膜液中IL-1β、IL-18含量及膝关节滑膜组织中NLRP3、JAK2、STAT3 mRNA表达均降低(P<0.05);与艾灸组比较，NLRP3过表达组左、右膝关节周长及膝关节滑膜液中IL-1β、IL-18含量增加(P<0.05)

膝关节滑膜组织中NLRP3、JAK2、STAT3 mRNA表达均升高(P<0.05)。结论:艾灸可能通过调控JAK2-STAT3信号通路发挥对AA家兔的抗炎消肿作用，其治疗效果可能与NLRP3表达密切相关。

Abstract

Objective To observe the effect of moxibustion on Janus protein tyrosine kinase 2/signal transducer and activator of transcription 3(JAK2-STAT3) signal pathway and interleukin(IL)-1β and IL-18 in synovial fluid of adjuvant arthritis(AA) rabbits

so as to explore the mechanism of moxibustion in the treatment of rheumatoid arthritis. Methods Twenty-eight rabbits were randomized into control

model

moxibustion

and NLRP3 overexpression groups

with 7 rabbits in each group. AA rabbit model was established by injection of Freund's complete adjuvant(FCA

0.5 mL/kg) into the rabbits' bilateral hind-knee joint cavities. On the third day after modeling

the NLRP3 lentiviral vector(40 μL) were injected into the bilateral hind-knee joint cavities of rabbits in NLRP3 overexpression group. Moxibustion was used to bilateral “Shenshu”(BL23) and “Zusanli”(ST36)

5 cones every time

once daily

6 days a week for 3 weeks in the moxibustion and NLRP3 overexpression groups. The perimeters of rabbits' hind legs were measured after modeling and after the intervention. The contents of IL-1β

IL-18 in synovial fluid were detected by ELISA and the expression levels of NLRP3

JAK2 and STAT3 mRNAs in synovial tissue were detected by real-time PCR. Results In comparison with the control group

the perimeters of bilateral knee joints were significantly increased at each time point(P<0.05)

and the contents of IL-1β

IL-18 in synovial fluid and the expression levels of NLRP3

JAK2

STAT3 mRNA in synovial tissue were significantly increased(P<0.05) in the model group. Compared with the model group

the perimeters of bilateral knee joints were significantly decreased(P<0.05)

and the contents of IL-1β

IL-18 in synovial fluid and the expression levels of NLRP3

JAK2

STAT3 mRNAs in synovial tissue were significantly decreased(P<0.05) in the moxibusion group. Compared with the moxibustion group

the above indexes were higher in the NLRP3 overexpression group(P<0.05). Conclusion Moxibustion may play an anti-inflammatory and detumescent role in AA rabbits by regulating JAK2-STAT3 signal pathway

and its therapeutic effect may be closely related to the expression of NLRP3.

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