Effect of electroacupuncture on Treg/Th17 balance in spleen and serum inflammatory factors in obese mice

LIU Yi; SHU Qing; LI Jin-xiao; ZHOU Min-feng; LIU Na

doi:10.13702/j.1000-0607.20211214

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Effect of electroacupuncture on Treg/Th17 balance in spleen and serum inflammatory factors in obese mice

更新时间：2023-08-11

- Effect of electroacupuncture on Treg/Th17 balance in spleen and serum inflammatory factors in obese mice
- Acupuncture Research Vol. 48, Issue 3, Pages: 240-246(2023)
- 作者机构：
  
  1. 武汉市红十字会医院康复科
  2. 武汉大学中南医院康复科
  3. 华中科技大学同济医学院附属协和医院中西医结合科
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.20211214
  CLC：
- Published：2023
- 稿件说明：
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LIU Yi, SHU Qing, LI Jin-xiao, et al. Effect of electroacupuncture on Treg/Th17 balance in spleen and serum inflammatory factors in obese mice[J]. Acupuncture research, 2023, 48(3): 240-246.
DOI：

LIU Yi, SHU Qing, LI Jin-xiao, et al. Effect of electroacupuncture on Treg/Th17 balance in spleen and serum inflammatory factors in obese mice[J]. Acupuncture research, 2023, 48(3): 240-246. DOI： 10.13702/j.1000-0607.20211214.

摘要

目的:以调节性T细胞(Treg)与辅助性T细胞17(Th17)平衡及血清相关炎性因子为切入点研究电针治疗肥胖的潜在机制。方法:雄性C57BL/6J小鼠随机分为正常组、模型组、电针组，每组10只。采用高脂饮食建立小鼠肥胖模型，电针组选取“足三里”“丰隆”“中脘”“关元”给予电针治疗，20 min/次，每周3次，共干预8周。观察各组小鼠进食量、体质量并计算Lee’s指数；多重液相芯片定量技术检测各组小鼠血清中白细胞介素(IL)-2、IL-4、IL-6、IL-10、IL-17A、γ干扰素(IFN-γ)、肿瘤坏死因子α(TNF-α)的表达；流式细胞术检测各组小鼠脾脏组织中Treg、Th17细胞水平；荧光定量PCR法检测各组小鼠脾脏组织中叉头框蛋白P3(Foxp3)、维甲酸相关孤儿受体(ROR-γt) mRNA的表达水平。结果:与正常组相比，模型组小鼠的进食量、体质量、Lee’s指数明显升高(P<0.01

P<0.001);血清中IL-2、IL-6、IL-17A、IFN-γ、TNF-α含量升高(P<0.01)

IL-4、IL-10含量降低(P<0.001

P<0.01);脾脏组织中Treg百分比及Foxp3 mRNA表达下降(P<0.01)

Th17百分比及ROR-γt mRNA表达升高(P<0.01)。与模型组相比，电针组进食量、体质量、Lee’s指数明显降低(P<0.01);血清中IL-2、IL-6、IL-17A、IFN-γ、TNF-α含量明显降低(P<0.01)

IL-4、IL-10的含量升高(P<0.01

P<0.05);脾脏组织中Treg百分比及Foxp3 mRNA表达升高(P<0.05)

Th17百分比及ROR-γt mRNA表达降低(P<0.01)。结论:电针可能通过调节肥胖小鼠脾脏Treg/Th17平衡及血清炎性因子的表达来改善其肥胖状态。

Abstract

Objective To observe the therapeutic effect of electroacupuncture(EA) on obese mice

and to explore the underlying mechanism of EA in treating obesity by focusing on the balance of regulatory T cells(Treg) and T helper 17 cells(Th17) and related inflammatory factors. Methods C57BL/6J male mice were randomly divided into normal group

model group and EA group

with 10 mice in each group. The obesity model was established by feeding the mice with high-fat diet. Mice in the EA group was treated with EA at “Zhongwan”(CV12)

“Guanyuan”(CV4)

“Zusanli”(ST36) and “Fenglong”(ST40) for 20 min every time

3 times every week

for a total of 8 weeks. The food intake and body weight of mice were observed and recorded

and Lee's index was calculated; the contents of interleukin 2(IL-2)

IL-4

IL-6

IL-10

IL-17A

gamma interferon(IFN-γ) and tumor necrosis factor(TNF)-α in serum were detected by multiplex liquid chip quantitative technique; the levels of Treg and Th17 cells in mice spleen tissues were detected by flow cytometry; and the expression levels of foxhead box p3(Foxp3) and retinoic acid related orphan receptor γt(ROR-γt) mRNA in spleen were detected by real-time quantitative PCR. Results Compared with the normal group

the food intake

body weight

Lee's index

the contents of IL-2

IL-6

IL-17A

IFN-γ and TNF-α in the serum

and the percentage of Th17 and expression of ROR-γt mRNA in the spleen tissues were significantly increased(P<0.01

P<0.001)

while the contents of IL-4 and IL-10 in the serum

the percentage of Treg and expression of Foxp3 mRNA in the spleen tissues were significantly decreased(P<0.001

P<0.01) in the model group. Compared with the model group

the food intake

body weight

Lee's index

the contents of IL-2

IL-6

IL-17A

IFN-γ

and TNF-α in the serum

the percentage of Th17 and expression of ROR-γt mRNA in the spleen tissues were significantly decreased(P<0.01)

while the contents of IL-4 and IL-10 in serum

the percentage of Treg and expression of Foxp3 mRNA in the spleen tissues were significantly increased(P<0.01

P<0.05) in the EA group. Conclusion EA may improve the obese state of mice by regulating the balance of Treg/Th17 in spleen and the expression of inflammatory factors in serum.

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