Effect of electroacupuncture at “Zusanli” (ST36) and “Tianshu” (ST25) on intestinal function and autonomic nerve balance in rats with irritable bowel syndrome
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Effect of electroacupuncture at “Zusanli” (ST36) and “Tianshu” (ST25) on intestinal function and autonomic nerve balance in rats with irritable bowel syndrome
WANG Zheng-wen, CHEN Bi-wei, CHEN Shao-zong, et al. Effect of electroacupuncture at “Zusanli” (ST36) and “Tianshu” (ST25) on intestinal function and autonomic nerve balance in rats with irritable bowel syndrome[J]. Acupuncture research, 2023, 48(2): 165-171.
DOI:
WANG Zheng-wen, CHEN Bi-wei, CHEN Shao-zong, et al. Effect of electroacupuncture at “Zusanli” (ST36) and “Tianshu” (ST25) on intestinal function and autonomic nerve balance in rats with irritable bowel syndrome[J]. Acupuncture research, 2023, 48(2): 165-171. DOI: 10.13702/j.1000-0607.20211324.
Effect of electroacupuncture at “Zusanli” (ST36) and “Tianshu” (ST25) on intestinal function and autonomic nerve balance in rats with irritable bowel syndrome
目的:观察电针“足三里”及“足三里”加“天枢”对肠易激综合征(IBS)大鼠结肠功能及自主神经平衡性的影响,探讨足三里配伍天枢治疗肠易激综合征是否具有协同作用。方法:Wistar大鼠随机分为对照组、模型组、足三里组、足三里+天枢组,每组14只。采用避水应激法建立IBS模型。足三里组、足三里+天枢组大鼠给予相应穴位的电针治疗,每次20 min
Objective To study whether electroacupuncture(EA) of “Zusanli”(ST36) combined with “Tianshu”(ST25) has a synergistic effect in regulating the colonic function and autonomic nerve balance in rats with irritable bowel syndrome(IBS). Methods Male Wistar rats were randomly divided into control
model
EA-ST36
and EA-ST36+ST25 groups
with 14 rats in each group. The IBS model was established by using water avoidance stress method. The visceral hypersensitivity was measured using the abdominal wall retraction reflex(AWR). The rectus abdominis electromyogram(EMG)
intestinal electrical activity
and electrocardiogram(ECG) were recorded using a PowerLab data acquisition and analysis system. The contents of serum cAMP and cGMP were determined by ELISA
the expression levels of colonic tyrosine hydroxylase(TH) and choline acetyl-transferase(ChAT) proteins were determined by immunofluorescence staining and Western blot
respectively. Results Compared with the control group
the model group had an evident increase in the levels of AWR
LF
LF/HF
ChAT protein expression
cAMP and cGMP contents and cAMP/cGMP ratio(P<0.001
P<0.05)
and a marked decrease in the levels of HF
frequency of slow waves of intestinal EMG
visceral pain threshold(PT)
immunoactivity and expression of TH protein(P<0.05
P<0.001). In contrast to the model group
the levels of AWR
LF
LF/HF
ChAT protein expression and immunoactivity
cAMP and cGMP contents and ratio of cAMP/cGMP were significantly reduced(P<0.001
P<0.05
P<0.01)
whereas the levels of frequency of slow waves of intestinal EMG
PT
and the immunoactivity and expression of TH were considerably increased(P<0.001
P<0.05) in both EA-ST36 and EA-ST36+ST25 groups. Conclusion EA of both ST36 and ST36+ST25 can relieve visceral pain
and reduce sympathetic activity to improve autonomic nerve balance
but without apparent synergistic effect between EA-ST36 and EA-ST25 in rats with IBS.
Effect of electroacupuncture of “Hegu” (LI4) and “Zusanli” (ST36) on intestinal sensitivity and motility in irritable bowel syndrome rats
Comparison of effects of electroacupuncture at “Tianshu” (ST25) and “Dachangshu” (BL25) on intestinal sensitivity and expression of muscarinic M_3R and 5-HT_(3A)R in irritable bowel syndrome rats
Electroacupuncture Relieves Visceral Hypersensitivity by Down-regulating Mast Cell Number,PAR-2/TRPV 1 Signaling,etc. in Colonic Tissue of Rats with Irritable Bowel Syndrome
Effect of Electroacupuncture at“Zusanli”(ST 36)on Vimentin (A Kind of Cytoskeleton Protein Related to Smooth Muscle Contraction) in Rats with Diarrhea-predominant Irritable Bowel Syndrome
Effect of Electroacupuncture at “Yintang” (GV 29) and “Tianshu” (ST 25) on the Ethology and the Expression of TRPV 1 Receptor in Colon of Irritable Bowel Syndrome Rats
Related Author
QIN Ying
GUO Meng-wei
LAN Ying
WANG Yi-fan
WANG Shan
JI Mao-xian
REN Xiao-xuan
ZHU Wen-lian
Related Institution
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine
College of Acupuncture-moxibustion and Massage,Hunan University of Traditional Chinese Medicine
Department of Rehabilitation,Chenzhou First People's Hospital
College of Acupuncture-moxibustion and Orthopedics-traumatology of Hubei University of Chinese Medicine,Hubei Provincial Collaborative Innovation Center for Preventive Treatment of Illnesses by Acupuncture and Moxibustion