Study on the mechanism of electroacupuncture repairing intestinal barrier via regulating mast cell in rats with diarrhea-predominant irritable bowel syndrome
|更新时间:2023-08-11
|
Study on the mechanism of electroacupuncture repairing intestinal barrier via regulating mast cell in rats with diarrhea-predominant irritable bowel syndrome
HOU Yu-jun, WANG Kai, JIANG Hui-ling, et al. Study on the mechanism of electroacupuncture repairing intestinal barrier via regulating mast cell in rats with diarrhea-predominant irritable bowel syndrome[J]. Acupuncture research, 2023, 48(3): 281-286.
DOI:
HOU Yu-jun, WANG Kai, JIANG Hui-ling, et al. Study on the mechanism of electroacupuncture repairing intestinal barrier via regulating mast cell in rats with diarrhea-predominant irritable bowel syndrome[J]. Acupuncture research, 2023, 48(3): 281-286. DOI: 10.13702/j.1000-0607.20220147.
Study on the mechanism of electroacupuncture repairing intestinal barrier via regulating mast cell in rats with diarrhea-predominant irritable bowel syndrome
Objective To observe the effect of electroacupuncture(EA) on mast cell activation-related substances and intestinal barrier function in diarrhea-predominant irritable bowel syndrome(IBS-D) model rats
so as to explore its underlying mechanisms. Methods Thirty female SD rats were randomly divided into control group
model group and EA group
with 10 rats in each group. IBS-D model was established by chronic unpredictable mild stress combined with senna solution gavage. Rats in the EA group received EA treatment(2 Hz/15 Hz
0.1-1.0 mA) at “Zusanli”(ST36)
“Taichong”(LR3) and “Tianshu”(ST25)
20 min per day
for a total of 14 days
with sides alternated daily. Visceral pain threshold was used to evaluate visceral hypersensitivity
diarrhea index was used to evaluate diarrhea degree. After all treatments
the pathological scores of colon were recorded after HE staining
the contents of cholecystokinin(CCK)
substance P(SP)
tryptase(TPS) and adenosine triphosphate(ATP) in colon were detected by ELISA
and the expressions of colonic tight junction protein ZO-1 and occludin were detected by Western blot. Results Compared with the control group
the visceral pain threshold
the expression levels of colonic ZO-1 and occludin proteins decreased(P<0.01)
while the diarrhea index
the contents of colonic CCK
SP
TPS and ATP were significantly increased(P<0.01) in the model group. After intervention
in comparison with the model group
the visceral pain thre-shold
the protein expression levels of colonic ZO-1 and occludin protein increased(P<0.01)
while the diarrhea index
the contents of colonic CCK
SP
TPS and ATP were significantly decreased(P<0.01) in the EA group. Conclusion EA can significantly alleviate the symptoms of visceral hypersensitivity and diarrhea in IBS-D rats. Its mechanism may be related to down-regulating colonic CCK
SP
TPS and ATP
inhibiting mast cell activation and degranulation
and up-regulating colonic barrier tight junction proteins.
Mechanisms of Peitu Yimu acupuncture in repairing intestinal mucosal barrier by regulating CRF/CRFR1 pathway in diarrhea-predominant irritable bowel syndrome rats
Effect of electroacupuncture on the morphology of prostate and degranulation of mast cells in rats with chronic nonbacterial prostatitis
Involvement of Pirt/TRPV1 signaling in acupuncture-induced reduction of visceral hypersensitivity in diarrhea-predominant irritable bowel syndrome rats
Effect of electroacupuncture on intestinal epithelial mucosal barrier function in rats with diarrhea-predominant irritable bowel syndrome
Related Author
WANG Kai
HOU Yu-jun
WANG Lu
LIAO Chen-xi
SONG Wei
CHEN Ying
ZHOU Si-yuan
ZHU Zhou
Related Institution
College of Acupuncture-Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine
College of Basic Medicine, Chengdu University of Traditional Chinese Medicine
Acupuncture-moxibustion and Tuina College, Guizhou University of Traditional Chinese Medicine
Department of Acupuncture-Moxibustion, Tuina and Traumatology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine
Department of Acupuncture and Moxibustion, Longhua Hospital, Shanghai University of Traditional Chinese Medicine