“The nerve growth factor (NGF)/phosphatidylinositol 3-kinase (PI3K)/transient receptor potential vanillic acid receptor 1 (TRPV1) signaling pathway has made important breakthroughs in the mechanism of acupuncture treatment of chronic visceral hypersensitivity in irritable bowel syndrome (IBS) with "Shang Ju Xu". Researchers established an adult chronic visceral hypersensitivity rat model and used various techniques such as abdominal wall pullback reflex (AWR) score, Western blot, and real-time fluorescence quantitative PCR to explore in depth the analgesic mechanism of acupuncture "Shangjuxu" on chronic visceral hypersensitivity in IBS. The experimental results showed that compared with the normal group, the AWR score of the model group rats under different pressure levels, as well as the mRNA and protein expression of NGF, TrkA, PI3K, and TRPV1 in the colon tissue, were significantly increased. After the intervention of acupuncture for "Shang Ju Xu", these indicators were significantly reduced, indicating that acupuncture for "Shang Ju Xu" has analgesic effects on chronic visceral hypersensitivity in IBS. This discovery provides a new theoretical basis for acupuncture treatment of IBS, and also provides more possibilities for the application of acupuncture and moxibustion in the treatment of chronic visceral hyperalgesia. This research achievement not only provides an important basis for the mechanism of nerve growth factor/phosphatidylinositol 3-kinase/transient receptor potential vanillic acid receptor 1 signal pathway in acupuncture treatment of IBS, but also provides strong support for the application and promotion of acupuncture and moxibustion in clinical practice. In the future, with in-depth research in this field, it is believed that the effect of acupuncture and moxibustion on IBS will be further improved, bringing good news to the majority of patients.”