最新刊期
卷 ， 期 1 ， 2002
- 摘要：Objective: To observe the effects of acupuncture on cerebral structure and enzymatic activity after cerebral ischemia and to analyze the mechanisms of acupuncture in improving stroke. Methods: 35 SD rats were randomized into sham operation group (n=6), ischemia group (n=9), ischemia+reperfusion group (n=6), acupuncture I group (n=6) and acupuncture II group (n=8). "Baihui"(GV 20) and bilateral "Quchi"(LI 11) were punctured and stimulated electrically with parameters of 7 Hz, 0.7 V and duration of 30 min, which was respectively given after inducing cerebral ischemia (30 min) in acupuncture I group and 3 hr after ischemia+reperfusion in acupuncture II group. Cerebral ischemia model was established using four artery (bilateral vertebral arteries and bilateral common carotid arteries) occlusion and reperfusion method. Glutathione peroxidase (GSH px), ATPase activity and malonyldialdehyde (MDA) in cerebral cortex and hippocampus tissues were assayed according to instructions of the reagent kits. Results: ① Following ischemia+reperfusion, cerebral tissue injury including neuron degeneration, death, gliocyte proliferation, etc was found in the hippocampus and cerebral cortex tissues in ischemia and ischemia+reperfusion groups, but in acupuncture II group no very apparent injury changes appeared. ②compared with the sham operation group, MDA contents in cellular plasma, membrane and mitochondria in ischemia group and ischemia+reperfusion group increased significantly in comparison with sham operation group (P<0.05, 0.001). Following acupuncture (both acupuncture I and II groups), MDA contents reduced significantly (P<0.001) compared with those of ischemia or ischemia+reperfusion groups (P<0.01). ③ Compared with the sham operation group, GSH px activity in cellular plasma, membrane and mitochondria increased in ischemia group (P<0.05); but compared with ischemia group, GSH px decreased very notably in ischemia+reperfusion group (P<0.01). After acupuncture, GSH px activity recovered basically compared with sham operation group. ④ Regarding ATPase activity, after ischemia and ischemia reperfusion, Na +,K + ATPase, Mg 2+ ATPase and Ca 2+ ATPase in plasma, membrane and mitochondria all lowered particularly in ischemia+reperfusion group (P<0.05). Following acupuncture (acupuncture II), the 3 types of ATPase activity in cellular membrane and mitochondria recovered completely while the 3 types of ATPase activity in plasma was even evidently higher than that of sham operation group (P<0.001). Conclusion: Acupuncture of "Baihui"(GV 20) and "Quchi"(LI 11) can effectively reduce cerebral ischemia reperfusion induced neuronal degeneration, death and hyperactivity of membrane lipid peroxidation, attenuation of free radical clearing ability and energy metabolic abnormality in the ischemic cortex and hippocampus .1025|0|0更新时间：2023-08-11
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- 摘要：Objective: To investigate the protective mechanisms of electroacupuncture (EA) on cerebral ischemic injury. Methods: SD rats were randomly and evenly divided into control group, sham operation group, ischemia(I) 2 hr group, I reperfusion (R) 2 hr group, I R 6 hr group, I R 12 hr group, I R 24 hr group, I R 2 hr+EA group, I R 6 hr+EA group, I R 12 hr+EA group and I R 24 hr+EA group (with 5 rats in each group). "Baihui"(GV 20) and "Shuigou"(GV 26) of the 4 ischemia+EA groups were stimulated with EA (20/4 Hz, 3 mA and duration of 1 hr). RT PCR was used to explore the effects of electroacupuncture (EA) on interleukin 1 receptor antagonist (IL 1Ra) mRNA expression in rats with middle cerebral artery occlusion (MCAo). Results: IL 1Ra mRNA expression was rapidly induced by MCAo, and increased significantly at 12 hr (0.770±0.076), reaching a peak level at 24 hr after reperfusion in the ischemic cortex area(0.994±0.098) compared with normal group (0.475±0.069,P<0.001). IL 1Ra mRNA also increased significantly after 12 hr IR in striatum (0.796±0.103) compared with normal group (0.521±0.061,P<0.05). However, IL 1Ra mRNA decreased after 24 hr IR in ischemic striatum (0.500±0.118). The expression of IL 1Ra mRNA in EA group increased significantly compared with ischemia control group after reperfusion 12 hr (0.890±0.087 vs 0.770±0.076 ) and 24 hr in cerebral cortex (1.201±0.155 vs 0.994±0.098 ) and 24 hr in stratium (1.046±0.132 vs 0.500±0.118),P<0.05,P<0.001. Conclusion: The results indicated that EA could upregulate the IL 1Ra mRNA expression in ischemia rats, that may contribute to the protective action of EA in reducing ischemic brain injury.1052|0|6更新时间：2023-08-11
- 摘要：Objective: To verify the effect of Orphanin (O) FQ on cerebral ischemia and observe its influence on electroacupuncture(EA)'s anti cerebral ischemia. Methods: 63 SD rats were randomly divided into sham operation (control) group (n=5), cerebral ischemia group (n=8), OFQ 10 μg group (n=7), OFQ 1 μg group (n=7), saline group (n=8), EA+ OFQ 1 μg group (n=8), EA+saline group (n=7) and EA group (n=6). Cerebral ischemia model was established using the cerebral middle artery occlusion (MCAo) method. "Shuigou"(GV 26) and "Baihui" (GV 20) were stimulated with EA (dense sparse waves, 3.85～6.25 Hz, 1.4～2 mA) for 60 min. Cerebral somatosensory evoked potential(SEP) was recorded and cerebral infarct volume detected. Thirty minutes after MCAo, OFQ (10 μg/10 μL, 1 μg/10 μL,0.1 μg/10 μL) and saline 10 μL were injected into the right lateral cerebroventricle via an implanted catheter. Results: Following cerebral ischemia, the amplitude of SEP decreased, and after intracerebroventricular (icv) administration of OFQ 10 μg and 1 μg respectively, SEP declined further. One hour after cerebral ischemia reperfusion, SEP recovered basically, while that of OFQ 10 μg group remained low level even 3 hr after ischemia reperfusion. A certain dose effect correlation was found between OFQ and SEP suppression reaction. Application of OFQ enlarged the infarct volume, but after icv of OFQ 0.1 μg, changes of the SEP amplitude and infarct volume had no significant difference compared with those of control group (P>0.05). Following icv of OFQ 10 μg and 1 μg, cerebral infarct volume increased considerably in comparison with that of cerebral ischemia group (P<0.05). Following cerebral ischemia, the amplitude of SEP in control group lowered from 71.46±6.42% to 27.64±6.26%, while in EA group, that of SEP decreased from 81.12±4.51% to 55.64±8,81%, existing a significant difference between these two groups (P<0.05); SEP amplitude of EA+OFQ group was significantly lower than that of EA+saline group (P<0.01). The cerebral infarct volumes in ischemia group and EA group were 24.18±1.08 mm 3 and 18.497± 5.112 mm 3 respectively. Following icv OFQ, the cerebral infarction volume EA+OFQ 1 μg group was remarkably larger than that of EA+saline group, suggesting an inhibitory action of OFQ on EA's anti cerebral ischemia. Conclusion: Orphanin FQ 10 μg and 1 μg (icv) deteriorates the degree of ischemic brain damage and reverses the anti cerebral ischemic effect of EA in the adult SD rats.923|0|1更新时间：2023-08-11
- 摘要：Objective: To observe the influence of acupuncture combined with medicine on the content of calmodulin(CaM) activity in rat ischemic cerebral region. Methods: The animal models of the cerebral ischemia with reperfusion were made by electric coagulation of bilateral vertebral arteries and occlusion of the ipsilateral common carotid artery. A total of 45 Wistar rats were randomly divided into five groups: ① normal control group (n=9), ② model control group(n=9),③ sham operation group (n=9), ④ electroacupuncture group[EA of"Shangyang"(LI 1), "Zhongchong"(PC 9), "Lidui" (ST 45) and "Zuqiaoyin"(GB 44), 2～20 Hz, 20 min, [WT5BX]n=9], ⑤ EA + medicine group (Venoruton 90 mg/200 g body weight was given by intragastric perfusion an hour before the model was made, , ⑤ EA + medicine group (Venoruton 90 mg/200 g body weight was given by intragastric perfusion an hour before the model was made, n=9). CaM activity of the ischmic cerebral tissues was measured by using phosphodiesterase method. Results:When cerebral ischemia occurred, CaM activity of the ischemic cerebral region elevated obviously in comparison with normal control group and sham operation group (P<0.05～0.001). Following administration of EA and EA + medicine, active CaM contents were significantly lower than that of model group (P<0.001). There was no evident difference between EA and EA + medicine groups in CaM contents (P>0.05). Conclusion: Both EA and EA+ medicine can reduce ischemia reperfusion induced increase of active CaM level in the rat.1168|0|1更新时间：2023-08-11
- 摘要：Objective: To reveal the mechanism of acupuncture in improving cerebral ischemia. Methods:A total of 120 Wistar rats were randomly divided into acupuncture (+ cerebral ischemia) group (n=36), cerebral ischemia group (n=36), sham operation group (n=36) and normal group (n=12). Cerebral ischemia was produced using middle cerebral artery occlusion (MCAo, thermocoagulation). Sixty minutes after MCAo, electroacupuncture of "Shuigou"(GV 26) and "Neiguan" (PC 6) was given to the rat by setting the parameters being frequency 4 ～10 Hz, 0.4 V, dense sparse waves and duration of 5 min. Fluorescence dyeing of the vascular endothelial cells and white blood cell fluorescein tracing were applied for observing changes of the appearance, density and blood flow velocity of the micrangia of the cerebral pia mater 3 hr, 6 hr and 24 hr after MCAo. All images were observed and recorded by microscope system of closed circuit TV and measured by computer image analyzer. Results: ① In normal group and sham operation group, the stained vascular endothelial cellular nuclei were very distinct, and the exudate staining was rather lighter. In cerebral ischemia group, the fluorescence staining of the vascular endothelial cells at different phases was lighter while that of the exudate was clearly denser, but in acupuncture group this state was obviously better; ② 3, 6 and 24 hours after MACo, the density of capillaries in the cerebral ischemic region of cerebral ischemia group decreased remarkably while that of acupuncture group was significantly higher than that of cerebral ischemia group (P<0.01). ③ 3, 6, 24 hours after MCAo, the blood flow velocity of cerebral ischemia group became very slow, while that of acupuncture group was remarkably faster than that of ischemia group (P<0.01). Conclusion: Acupuncture can reduce ischemic injury of the vascular endothelium and improve the cerebral microcirculation effectively and immediately.978|0|7更新时间：2023-08-11
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- 摘要：Objective: To observe the effect of electroacupuncture (EA) on changes of amino acids in the ischemic cerebral hemorrhage (ICH) rat. Methods: Collagenase and heparin were injected into caudate nucleus to establish ICH rat model. The effect of EA on the content of amino acid in the brain tissue was observed. Results: The content of EAA (excitatory amino acid, ASP, Glu)and IAA(inhibitory amino acid, GABA) in the brain tissue of the model group were raised, the EAA/IAA was imbalance, the content of Glu and GABA in the brain tissue of the acupuncture "Shuigou" (DU 26) group were reduced and that was significantly compared with that of the model group(P<0.05). The contents of ASP and Glu in the brain tissue of the acupuncture "Fengfu" (DU 16) group were reduced significantly compared with that of model group (P<0.05). Conclusion:Acupuncture of"Shuigou"(DU 26) and "Fengfu"(DU 16) can reduce the release of EAA(Glu,ASP) and balance EAA/IAA, that may relieve ICH induced brain tissue injury.1179|0|3更新时间：2023-08-11
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- 摘要：Objective: To observe the effect of acupoint stimulation on the internal organs under experimental dorsal root reflex and axonal reflex. Methods: 29 SD rats were randomly divided into control group (n=8), experimental group (n=13) and casaicin group (n=8). A transverse incision operation of spinal cord between T 12 and T 13 was performed in these rats and all the ventral roots below T 13 were amputated. Evans fluid (40 mg/kg) was injected intravenously via the external jugular vein. Left "Zusanli"(ST 36) was stimulated electrically for 30 min with a concentric electrode by setting the stimulating parameters of wave width being 0.5 ms, strength 15 mA and frequency 5 Hz. Rats of capsaicin group were treated by injecting capsaicin 50 μL(66 mMol) into "Zusanli"(ST 36) 6 days before each experiment for injuring fine afferent nerve fibers. At the end of each experiment, the rats were killed for opening the abdominal cavity and observing exudation state in the internal organs. The neurogenic inflammation was considered to be the index of the activity of meridian. Results: Findings showed that under the two reflex conditions, the Evans blue content of the visceral organs such as liver, spleen, pancreas and the whole gastrointestinal tract increased significantly compared with that of control group(P<0.01) following electrical stimulation of "Zusanli"(ST 36). The exudation was not evident in the lung, heart, bladder, kidney, ureter, spermatic duct, testicle, etc. No significant differences were found between experimental group and control group in Evans blue content in bladder, testicle, etc. The present study provided a new way to study the correlation between meridian and visceral organs. In capsaicin group, no significant differences were found between capsaicin and control group in the Evans blue contents in these organ tissues (P>0.05), but in comparison with experimental group, Evans blue contents in liver, spleen, pancreas and gastrointestinal tract lowered apparently (P<0.01). Conclusion: The fact suggests that the neurogenic inflammation of the viseral organs evoked by electrical stimulation of acupoint is mediated by the capsaicin sensitive afferent fibres.1002|0|15更新时间：2023-08-11
- 摘要：Objective: To investigate effects of electroacupuncture (EA) of "Zusanli" (ST 36) on changes of SP immunereactivity (IR) and VIP IR contents in rats' pituitary gland and peripheral blood as well as on cellular immune, and to explore the regulatory effect of EA on the nerve endocrine immune regulatory network. Methods: 40 SD rats were evenly randomized into normal control, "Zusanli" EA, immunosuppressive model, non acupoint and "Zusanli"+immunosuppressive groups. Immunosuppressive model was established by intraperitoneal (ip)injection of cyclophosphamide (CY,100 mg/kg). Non acupoint was located about 0.5 cm beside "Zusanli" (ST 36). Both "Zusanli" and non acupoint were punctured and stimulated with electrical parameters of frequency 2 Hz, strength 3 V, intermittent waves and duration of 30 min. Acupuncture treatment was given once daily continuously for 7 days. Changes of SP IR and VIP IR contents in the pituitary gland and peripheral blood were determined by radioimmunoassay. By using micro whole blood direct immunofluorescence staining and flow cytometer T lymphocyte subpopulations were assayed. Erythrocyte immunity indexes including red blood cell C 3b receptor rosette (RBC C 3bRR) and red blood cell immune complex rosette (RBC ICR) were determined using immunoadhesion yeast rosette method. Results: Following ip of CY, SP and VIP contents in blood and pituitary gland, CD + 4% and RBC C3bRR rate reduced significantly in comparison with normal control group (P<0.01). Hypophysis and blood SP and VIP contents, percent of CD + 4, RBC C 3bRR and RBC ICR in "Zusanli" group were significantly higher than those of control group (P<0.01). While CD + 8% in "Zusanli" and immunosuppressive groups had no evident changes compared with control group (P>0.05). In addition, both changes of CD + 4 and SP and VIP contents, and those of CD + 4 and RBC C3bRR presented a significantly positive correlation (P<0.05～0.01). There were no significant differences between "Zusnali" +immunosuppressive group and control group, and between non acupoint group and control group in the above mentioned indexes (P>0.05).Conclusion: Acupuncture can raise the immunity of the body , that may be closely associated with the increased synthesis and release of SP and VIP in thepituitary gland and peripheral blood, facilitating modulation of the nerve endocrine immune regulatory network.1696|0|6更新时间：2023-08-11
- 摘要：Objective: To reveal the influence of electroacupuncture (EA) on celluar immune function in rats. Methods: According to blocked randomization method thirty two Sprague Dawley rats were randomly divided into control group, EA group, model group and model + EA group. Immunosuppressive model was established by intraperitoneal (ip) injection of cyclophosphamide (CY,100 mg/kg, twice). Bilateral "Zusanli"(ST 36) were punctured with filiform needles and stimulated with an EA therapeutic apparatus, once daily, continuously for 7 days. T lymphocyte subgroups of peripheral blood including CD 4 + and CD 8 + were measured by direct microvolume whole blood immunoflurescent staining technique and a flow cytometer, and erythrocyte immune indexes including RBC C 3bRR and RBC ICR were analyzed using immunoadhesion yeast rosette method. Results: ① Following ip of CY, the values of RBC C 3bRR and CD 4 + in model group were obviously lower than those of control group (P<0.05, 0.01), and no significant difference was found between two groups in RBC ICR and CD 8 + (P>0.05),indicating that CY restrained the erythrocyte immune function and T lymphocyte subgroups of peripheral blood. ② Following acupuncture, the values of CD 4 +, RBC C 3bRR and RBC ICR in EA group raised significantly in comparison with those of control group(P<0.01),indicating that EA had a considerable regulative effect on the immune function. But CD 8 + had no apparent changes in comparison with control group (P>0.05). There was a positive correlation between changes of CD 4 + and RBC C 3bRR(r=0.719,P<0.05). ③ The values of RBC C 3bRR, RBC ICR and CD 4 + in model +EA group were significantly higher than those of model group and control group (P<0.05, 0.01). Conclusion: EA of "Zusanli"(ST 36) point can improve cell mediated T lymphocyte and red blood cell immunoadhesion functions in rats with immunosuppression.1030|0|4更新时间：2023-08-11
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