摘要:Objective: To explore the morphological connective pathways between the cardiac Shu and Mu acupoints and the heart. Methods: Thirty Wistar rats anesthetized with pentobarbital (40 mg/kg) were randomly and equally divided into "Xinshu" (BL 15), "Juque" (CV 14) and "Xinshu" "Juque" groups. 5% Propidium Iodide (PI, 15 μL) was separately injected into the acupoint areas and 10% Bisbenzimide (Bb, 20 μL) was injected into the heart following thoractomy under anesthesia in each group. After surviving for 12 hours, the rats were anesthetized with pentobarbital again and infused with saline and 4% paraformaldehyde phosphate buffer solution respectively, followed by taking out the spinal dorsal root ganglions to be cut into sections (40 μm thick). Under microscope, the labeled neurons were determined.Results: Results indicated that, the mono labeled neurons of "Xinshu" (BL 15) and heart were located in the ganglions of T 1~T 6 and T 2~T 5 respectively both with T 5 being predominant. The doubly labeled neurons were located in the ganglions of T 2~T 5, with T 5 being predominant. The mono labeled neurons from "Juque" (CV 14) were located in T 1~T 7 ganglions and the doubly labeled neurons in T 2~T 5 ganglions, also with T 5 being predominant. Conclusion: T 2~T 5 ganglions, predominantly T 5 ganglions, are one of the morphological base for the specific connection between cardiac Shu and Mu acupoints and the heart.
摘要:To explore the neural connection pathway between the kidney and its back shu and front mu points. Methods: 18 Wistar rats anesthetized with pentobarbital (40 mg/kg) were randomly and equally divided into "Shenshu" (BL 23), "Jingmen" (GB 25) and "Shenshu" "Jingmen" groups. Propidium Iodide (PI, 5%, 15 μL) was separately injected into the left "Shenshu" (BL 23) and "Jingmen" (GB 25) areas and 10% Bisbenzimide (Bb, 20 μL) was injected into the left kidney following laparotomy in each group. After surviving for 14 hours, these rats were anesthetized again and infused with normal saline, 4% paraformaldehyde phosphate buffer solution through the aorta. Then the spinal dorsal root ganglions (DRG) of T 9~L 6 segments were collected to be cut into sections (40 μm thick) for detecting the labeled neurons under light microscope. Results: In "Shenshu" group, the mono labeled cells from the acupoint and the kidney were found in DRG of T 11 ~L 5 (being densest in L 1~L 3) and T 12 ~L 4 (being densest in L 1~L 3) respectively; while the doubly labeled neurons founded in T 13 ~L 3 (peaking between L 1~L 2). In "Jingmen" group, the mono labeled cells from GB 25 were found in DRG of T 10 ~L 3 (being densest in T 12 ~L 1), while the doubly labeled neurons founded in T 12 ~L 2 (peaking in T 12 and T 13 ). In "Shenshu" "Jingmen" group, the doubly labeled neurons founded in T 13 ~L 1. Conclusion: T 12 ~L 2 DRGs are the important connective links between "Shenshu", "Jingmen" and the kidney, which also reveals the biological essence of "Qijie" doctrine in TCM.
摘要:Objective:To explore the mechanism of the formation of infrared radiant track along meridian course(IRRTM). Methods:The oxygen partial pressure in 5 acupoints Yaoyangguan (GV 3), Mingmen (GV 4), Zhongshu (GV 7), point below T 8 process and Shenzhu (GV 12)along Du Meridian and the bilateral control points 2.5 cm to Du Meridian (5 levels) were taken to be observed in twenty eight volunteer subjects. At the same time, the image of infrared radiant track over the back of these subjects was recorded. Results:Besides the higher temperature infrared radiant track of Du Meridian, the oxygen partial pressure (PO 2) values in the deep tissues along Du Meridian are 8.13±0.61 kPa, 7.63±0.55 kPa, 8.07±0.57 kPa, 6.90±0.41 kPa and 7.95±0.68 kPa, being significantly higher than those of the left and the right control points (6.30±0.53 kPa and 5.46±0.43 kPa, 5.25± 0.45 kPa and 5.93±0.46 kPa, 6.35±0.51 kPa and 6.18±0.50 kPa, 5.48±0.46 kPa and 4.80± 0.49 kPa, and 5.85±0.57 kPa and 5.63±0.51 kPa respectively, P <0.05~0.001). The results suggest that higher PO 2 along higher temperature infrared radiant track of meridian means higher oxygen metabolism rate, which may be an important factor for the formation of IRRTM, and maybe contribute to the transformation and transmission of signals of meridians and to the regulation of functional activity of the human body.Conclusion:PO 2 is obviously higher along the higher temperature infrared radiant track of meridian.
摘要:Objective: To observe the effect of acupuncture on pituitary gonadotropic hormones and ovariogenic hormones in the peripheral blood in healthy women of childbearing age. Methods: 60 questionnaires on basal body temperature were given to 60 females at the age of 22~35 years for observing the variation of basal body temperature and menstrual cycle within 2 menstruation periods, and 28 volunteer females with regular menstruation and basal body temperature presenting typical ovulation were selected as the subjects in this study.Before acupuncture treatment, blood samples were taken and kept in the fridge one day before and on the day of the calculated ovulation. These 28 subjects accepted acupuncture stimulation during the forth menstruation period, beginning from the first 3 days on before ovulation, once daily continuously for 4 days, followed by taking blood samples. Auricular and body acupoints used were G 4 Area (both sides), Shimen(CV 5) and Sanyinjiao (SP 6,one side). After inserting needles into bilateral G 4, rotated the needles for 1 min, then punctured the body acupoints till obtaining needle sensations, afterwards twisted and thrust the needles for 1 min. Then, Shimen (CV 5) and Sanyinjiao(SP 6) were stimulated electrically for 15 min with an Electroacupuncture Therapeutic Apparatus. Results: Results indicated that before and after acupuncture, the concentration values of follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the peripheral blood were 3.90±2.30 IU/L and 11.54±11.73 IU/L, 3.38±1.76 IU/L and 8.68±8.15 IU/L respectively, no significant differences were found between pre and post acupuncture ( P >0.05), meaning no effect of acupuncture on pituitary gonadotropic hormones. Before and after acupuncture, the concentrations of blood estradiol (E 2), testosterone (T) and pregnendione (P) were 0.54± 0.42 nmol/L, 6.84±2.88 nmol/L and 3.86±2.76 ng/L, 0.35±0.21 nmol/L, 5.03±2.16 nmol/L and 2.97±2.05 ng/mL separately, showing considerable differences between pre and post acupuncture in each index ( P <0.01, 0.001 and 0.05). It displays that acupuncture possesses an inhibitory effect on the secretion activities of ovariogenic hormones.Conclusion: Acupuncture stimulation has a suppressing effect on blood E 2, T and P in healthy women of childbearing age.
摘要:Objective:To observe the therapeutic effect of acupuncture needle retaining combined with cupping and patient's exercise for treatment of traumatic shoulder pain. Methods: 45 outpatients were randomly divided into treatment group ( n =25) and control group ( n =20). In treatment group, the patients were treated by acupuncture of the contralateral Tiaokou (ST 38) to Chengshan (BL 57) of the affected shoulder. The needle was retained for 10 min, when, ordered the patient to perform the lateral lifting, upward lifting, frontward stretching and backward bending movement of the affected upper limb, and manipulated the needle twice. In addition, the tender point, Tianzong (SI 11) or Jianqian Point or Jianyu (LI 15) to Jiquan (HT 1) were also punctured according to the concrete circumstances, combined with cupping at the focus site, then, asked the patient to make "wall climbing" movement closely facing the wall of a room. In control group, warming needle of LI 15, Jianzhen (SI 9), Jianqian, Quchi (LI 11), Hegu (LI 4), etc were given. The treatment was conducted once daily, with 5 sessions being a therapeutic course. Results: After three courses of treatment, of the 25 and 20 cases in the treatment and control groups, 16 and 10 were cured, 7 and 8 had remarkable improvement, with the effective rates being 92% and 90% respectively. No significant difference between the two groups was found ( P >0.05), but the therapeutic courses of the former group were significantly shorter than those of the later group in most patients ( P <0.05). Conclusion: The therapeutic effects of treatment group are superior to those of control group in pain relief and treatment duration.
摘要:In the present paper, the author expounds the clinical application of the "fifteen connecting acupoints " by combining typical cases including those with arrhythmia, toothache, chest distress with irritability, deaf with tinnitus, radial nerve injury, numbness of both knees with difficulty in walking, cerebral traumatic injury induced disturbance of the limb activities, knee joint pain, migraine, progressive lower limb paralysis, costal chondritis, proctoptosis, etc. and introduces the clinical experiences. In accordance with the clinical practice, the author holds that the "fifteen connecting acupoint system" is an entirety system and has a unique therapeutic effect in treatment of some diseases.
摘要:To study the neuropeptides involved in the peripheral information transmission across dermatomes and the relevant mechanisms. Methods: Wistar rats are used in this study. Stimulate one nerve innervating a acupoint of "Pangguang Meridian of Foot Taiyang" antidromically and record the discharge of the other nerve innervating the neighboring acupoint next to or several segments away from the stimulated nerve. Using the model of the information transmission across dermatomes, the discharges from the acupoint are observed by treatment respectively with capcaisin (destroying the peptidergic nerves), substance P (SP), calcitonin gene related peptide (CGRP), neuropeptide A (NKA) and NK 1 receptor antagonist and H 1 receptor antagonist. Results: This study proves for the first time that imitating acupoint stimulation may induce information transmission across dermatomes along the route of meridian. The characteristics of the information transmission across dermatomes is accord with regularities of propagation of sensation along meridians (PSM), that may contribute to the phenomenon of PSM in clinic. The information transmitted across dermatomes decreased significantly after treatment with capcaisin to destroy the peptidergic nerves. SP, CGRP and NKA facilitated the information transmission across dermatomes and the intensity and duration of the effect are SP>CGRP>NKA. NK 1 receptor antagonist (WIN 51,708) and H 1 receptor antagonist (benzhydramine) decrease the information transmitted across dermatomes. These results suggest that SP, CGRP, NKA, histamine, etc. may contribute to the information transmission across dermatomes via combining with their receptors. The activation of sympathetic nerve may enhance the information transmission. Conclusion: Multiple types of neuropeptides and their receptors are involved in PSM.
摘要:In the present paper, the authors put forward their viewpoint on the channel system of TCM by reviewing individual long term clinical observation and making objective analysis on abundant literature. Channels, also called as energy channels, are the objectively existing substance system and have their stereoscopic structure. Channels can function in transmitting various information, carrying qi and blood to every part of the body. Qi and blood may be considered to be a kind of energy.
摘要:In the present paper, the author puts forward a hypothesis that the meridian system may be an interstitial pressure band on the basis of the theory of traditional Chinese medicine including entirety concept, correspondence between man and universe, balance between yin and yang, etc, clinical practice and relative concept thinking.