最新刊期
卷 ， 期 1 ， 2004
- 摘要：Objective:To observe the effect of changes of hypothalamic β EP level and expression of pro opiomelanocortin (POMC)mRNA on aftereffects of acupuncture analgesia.Methods:132 SD rats were evenly randomized into control group, model group and acupuncture group, and each group was then divided into 0 min, 15 min, 30 min, 1 hr, 2 hr, 4 hr, 8 hr, 12 hr, 18 hr and 24 hr subgroups, with 8 cases in each of the former 4 subgroups and 2 cases in each of the latter 6 subgroups. Rat inflammatory pain model (acute adjuvant arthritis) was made by injecting Freund's complete adjuvant (0.1 mL) into the right hindpaw. Right“Kunlun"(BL 60) was punctured with a filiform needle and retained for 30 min, when, the needle was manipulated once again every 5 min. Then, the pain threshold (latency of hindpaw withdrawal in responding to radiation heat stimulation) of the right hindpaw was determined with a radiation heat pain detector. Hypothalamus tissues of some rats of the abovementioned 10 subgroups were isolated to be cut into sections (6～8 μm) that were stained with in situ hybridization technique for displaying expression of POMC mRNA, and β EP content of hypothalamus tissue was determined with radioimmunoassay. Results:The pain threshold values of model group at 0 min, 15 min, 30 min and 1 hr were significantly lower than those of control group ( P < 0.01 ), and those of acupuncture group were all considerably higher than those of model group ( P < 0.01 ) despite being still markedly lower than those of control group at 0 min and 1 hr ( P< 0.05, 0.01 ). Comparison among the 3 groups showed that β EP contents of model group and acupuncture group at 0 min, 15 min, 30 min and 1 hr were significantly higher than those of control group ( P < 0.05,0.01 ); and those of acupuncture group were even markedly higher than those of model group at 0 min, 15 min and 1 hr ( P < 0.05,0.01 ). Changes of POMC mRNA expression in these 3 groups presented a similar tendency to those of β EP contents. Conclusion: Acupuncture has immediate effect and remarkable aftereffect in relieving pain, which may be closely related to acupuncture induced increase of β EP content and up regulation of POMC mRNA expression in the hypothalamus.1037|0|7更新时间：2023-08-11
- 摘要：Objective:To observe the effect of acupuncture of “Baihui"(GV 20) and“Dazhui"(GV 14) on the activity of glutathione peroxidase (GSH PX) and the neuronal apoptosis in hippocampal region in vascular dementia rats. Methods:Thirty six Wistar aging rats were randomly and evenly divided into sham operation, model, acupuncture and medication groups. Vascular dementia model was established by occlusion of the bilateral common carotid arteries and reperfusion. In acupuncture group, “Baihui"(GV 20) and “Dazhui"(GV 14) were punctured with filiform needles and stimulated for 5?min by twirling the needles swiftly. In medication group, the rats were fed with Piracetum liquid (40 mg/mL, 6 mL/kg) via gastric tube. The treatment of two groups was conducted once daily, continuously for 30 days. GSH PX activity of the hippocampus was determined with TdT mediated dUTP biotin nick end labeling technique and apoptosis was detected with TUNEL method. Rats' behavior reaction was tested with active avoidance method (shuttle box). Results: The foot shock times and duration of model group were significantly more and longer than those of sham operation group ( P <0.001), while in comparison with model group, these two indexes of acupuncture and medication groups were significantly lower ( P <0.01). No significant differences were found between acupuncture and medication groups in these two indexes, suggesting that both acupuncture and medication can improve the memory of VD rats. In comparison with sham operation group, GSH PX activity decreased significantly ( P < 0 001 ); whereas compared with model group, GSH PX activity of both acupuncture and medication groups increased considerably ( P <0.01), and the effect of acupuncture group was significantly superior to that of medication group ( P <0.05). Results of HE staining indicated that in model group, there were a large number of apoptotic neurons in CA 1 area of the hippocampus, while the situation was apparently better in acupuncture and medication groups. Conclusion: Acupuncture of “Baihui" and “Dazhui" can apparently ameliorate the learning and memory ability of VD rats, raise GSH PX activity and reduce neuronal injury of the hippocampal region.1120|0|9更新时间：2023-08-11
- 摘要：Objective:To observe the effect of acupuncture on ischemic cerebral neurons and to investigate its molecular mechanisms in reducing cerebral ischemic injury. Methods: Forty SD rats were randomly and evenly assigned to model group and acupuncture group. Cerebral ischemia model was established by middle cerebral artery occlusion. Electroacupuncture (EA, 2 Hz, 1 mA, duration of 60 min) was applied to “Shuigou”(GV 26), “Neiguan”(PC 6) and “Baihui”(GV 20). Sections of the cerebral tissue were stained by using immunohistochemical (ABC) method. The number of apoptosis and Bcl 2 expression were observed under microscope. Results: The mean numbers of apoptotic cells of the local ischemic cerebral region in model group and acupuncture group were 7.23±1.50 and 3.16±1.12 respectively, showing that the apoptotic cells of acupuncture group were significantly fewer than those of model group. The mean numbers of Bcl 2 immunoreactive (IR) positive neurons of model and acupuncture groups were 42±21 and 89±14 separately, with the number of Bcl 2 IR positive neurons of acupuncture group being markedly more than that of model group ( P <0.05). Conclusion: Acupuncture could apparently suppress cerebral ischemia induced apoptosis and up regulate Bcl 2 protein expression,that may contribute to the protective effect of acupuncture on ischemic cerebralneurons.1046|0|4更新时间：2023-08-11
- 摘要：Objective: To observe the effect of scalp acupuncture, body acupuncture and joint administration of acupuncture and medicine on cerebral ischemia induced changes of calmodulin (CaM) activity. Methods: 63 Wistar rats were randomly and evenly divided into control, sham operation, model, scalp acupuncture (SA), body acupuncture (BA), SA+medicine and BA+medicine groups. Cerebral ischemia reperfusion (IR) model was established by occlusion of the bilateral vertebral arteries and common carotid artery. Scalp points “Shuigou"(GV 26) and “Baihui"(GV 20), and body acupoints “Shangyang"(LI 1) and “Zhongchong"(PC 9) were punctured and stimulated electrically (20 Hz, 1 mA, continuous waves) for 20 min. In acupuncture+medication group, intra gastric perfusion of Venoruton (0.45 g/kg) was given to the rats. CaM concentration in the brain was determined with radioimmunoassay. Results: In comparison with control and sham operation groups, CaM content of model group increased considerably ( P <0.001). Compared with model group, CaM contents of SA, BA, SA+medicine and BA+medicine groups all decreased significantly ( P <0.01～0.001). In comparison with SA group, CaM contents of both SA+medicine group and BA+medicine group were significantly lower ( P <0.05), but no significant differences were found between control group and sham operation group, among BA, SA+medicine and BA+medicine groups, and between SA and BA groups in CaM contents ( P >0.05). Conclusion: Both scalp acupuncture and body acupuncture and joint administration of acupuncture and medicine can significantly lower CaM content of the brain tissue in cerebral ischemia rats, and the effect of SA+medicine is apparently superior to that of simple acupuncture.1097|0|1更新时间：2023-08-11
- 摘要：Objective:To explore methods for establishing pathological model of “Qi arriving at the affected area" of traditional Chinese medicine, and to try to provide a clue for further studying about the essence of meridians. Methods: Thirty male rabbits anesthetized with 20% urethane (1 g/kg) were randomly divided into experimental group ( n =15) and control group ( n =15). Silver ball electrodes were placed separately below the spinous processes of C 7, T 3, L 4 and L 6 for recording the spinal evoked potential (SEP) after electrical stimulation of the exposed heart for 15 sec (for inducing pathological changes, the artificial focus) and subsequent electroacupuncture (EA) stimulation of bilateral “Zusanli"(ST 36). The reference electrode was placed beneath the subcutaneous tissue. Ten minutes later, Nitrate Strychnine (2 mL, an inhibitor of glycine) was injected into the subcutaneous tissues for enhancing the excitability of spinal cord. The treatments of control group were the same to those of experimental group except stimulation of the heart. Results: In treatment group, before cardiac stimulation, SEP could be recorded only from L 4 and L6; following cardiac stimulation, SEP could also be recorded from T 3 and C 7 (fewer cases). After administration of Nitrate Strychnine, SEP could be recorded from C 7 in every animal. Along with the appearance of SEP from C 7, the post stimulation electrocardiogram was also improved. In control group, following acupuncture stimulation of ST 36, SEP was recorded from L 4 and L 6; after subcutaneous injection of Nitrate Strychnine, SEP could also be recorded from T 3. Conclusion: The conducting SEP under background of subcutaneous administration of Strychnine may be employed as a model of “Qi arriving at the affected area".1009|0|0更新时间：2023-08-11
- 摘要：Objective: To investigate changes of activities of c fos and nitric oxide synthase (NOS) in visceral afferent pathway during gastric experimental inflammation pain and following electroacupuncture (EA). Methods: A total of 30 SD rats were evenly randomized into control group, inflammatory pain group (model group) and EA group. Inflammatory pain model was generated by intragastric infusion of formaldehyde plus normal saline. “Zusanli" (ST 36) was punctured and stimulated electrically (1～5 V, 4～16 Hz) for 60 min. Immonohistochemical staining techniques were used to detect the activities of c fos and NOS in the nodose ganglion(NG), dorsal root ganglion(DRG), spinal cord(SC)and the nucleus of the solitary tract(NTS)during gastric inflammation pain and after EA. Results: In comparison with control group, the number of c fos immuno reaction (IR) positive neurons in NTS X of model group and EA group was significantly bigger ( P <0.01); while that of EA group was significantly smaller than that of model group ( P <0.01). No significant changes were found in SC of the 3 groups. The proportions of NOS positive (stronger and moderate positive) neurons of NG and DRG in model group were significantly lower than those of control group ( P <0.01), while those of NOS positive (stronger, moderate and weaker positive) neurons in NG and DRG of EA group were significantly higher than those of model group ( P <0.01). It shows that NO may be involved in the afferent process of gastric pain signals ascending to the brain stem by way of visceral sensory pathway along the vagus nerve, and EA may reduce pain induced expression of NOS and c fos.Conclusion: EA can resist gastric nociceptive stimulation generated increase of NOS and c fos activities. The descending inhibitory pathway existing in the spinal cord may check the ascending transmission of the visceral nociceptive information.958|0|2更新时间：2023-08-11
- 摘要：Objective:To observe the effect of electroacupuncture (EA) of “Zusanli(ST 36) on expression of Bcl 2 and Fas proteins in alcohol induced gastromucosal lesion rats for analyzing the relationship between cytoprotection and expression of Bcl 2 and Fas proteins, and the point relative specificity.Methods:40 Wistar rats were randomly and evenly divided into “Zusanli"(ST 36),non acupoint, model and control groups. Gastric mucosal injury model was induced by intragastric infusion of dehydrated alcohol (1 mL per animal). The expression of Bcl 2 and Fas proteins in each group were detected with immunohistochemical techniques.: Expression of Bcl 2 protein of gastric mucosa tissues in “Zusanli" group was obviously stronger than that of model group( P <0 05 ).Expression of Fas protein in “Zusanli" group was decreased significantly in comparison with model and non acupoint groups ( P <0 01), suggesting that acupuncture of “Zusanli" generated up regulation of Bcl 2 expression and down regulation of Fas expression may contribute to its effect in protecting the gastromocosal tissue from injury and the effect of acupuncture of “Zusanli" is of relative specificity.:Electroacupuncture of “Zusanli"(ST 36) can up regulate expression of Bcl 2 and down regulate expression of Fas protein of gastromucosal tissues and the effect of “Zusanli"(ST 36) possesses a relative specificity.1188|0|3更新时间：2023-08-11
- 摘要：Objective:To research the effects of acupuncture manoeuvres of “regulating mentality and tonifying kidney”(RMTK) on the morphology and function of serotonergic neurons in the midbrain of climacteric rats. Methods: Forty five female Wistar rats (aged 16 months) were randomly divided into climacteric group ( n =15), RMTK group ( n =15),“reinforcing kidney and invigorating spleen”(RKIS) group ( n =15), additionally, other 15 young female rats (4 months old) were made up of control group. In RMTK group, “Fengfu”(GV 16), “Baihui" (GV 20), “Qihai”(CV 6) and “Shenshu”(BL 23) were punctured , with the former two acupoints being stimulated by lifting, thrusting and twirling the needles with moderate amplitude, and the later two acupoints being stimulated with smaller amplitude. In RKIS group, “Shenshu”(BL 23), “Zusanli”(ST 36) and “Sanyinjiao”(SP 6) were punctured and stimulated by lifting, thrusting and twirling the needles with small amplitude. The treatment was conducted once daily, continuously for 4 weeks. These rats’ brain tissues were cut into sections to be stained with immunocytochemical technique (SABC method). 5 HT like immunoreaction (IR) positive products were observed under microscope and assayed with computerized image processing system to determine the total area and the integrated optical density of 5 HT like IR positive substances in B 7 and B 8 of the midbrain. Results:Compared with the average area of IR positive products and the average integrated optical density of control group, those of climacteric group decreased significantly ( P <0.01), suggesting a decline of 5 HT synthesis and secretion in B 7 and B 8 of the brain in climacteric rats. After administration of acupuncture, the average area of IR positive products and the average integrated optical density of RMTK group were markedly higher than those of climacteric and RKIS groups, suggesting that needling manoeuvres of “regulating mentality and tonifying kidney”are superior to those of “reinforcing kidney and invigorating spleen”needling in potentiating activity of serotonergic neurons including increase of the synthesis of 5 HT. Conclusion:Acupuncture intervention of “regulating mentality and tonifying kidney”technique may potentiate expression of serotonergic neurons in climacteric rats.1131|0|3更新时间：2023-08-11
- 摘要：Objective: To observe the effect of electroacupuncture (EA) of "Zusanli"(ST 36) on nitric oxide synthase (NOS) mRNA expression in the brain in rats with morphine abstinence syndrome for studying the biomolecular mechanism of acupuncture in improving abstinence syndrome. Methods: Rat abstinence syndrome model was established by continuous muscular injection of morphine to the hindlimbs for 15 days. A total of 24 rats were randomly and evenly assigned to abstinence syndrome (AS) group, EA (ST 36) group and L nitroarginine (NAME) group. Expression of nNOS mRNA in the brain tissues was determined by using the reverse transcription polymerase chain reaction (RT PCR) method. EA (2/100 Hz, 10 V, duration of 30 min/time, once daily and 7 days altogether) was delivered to bilateral ST 36 via two inserted needles. Results: Comparison of abstinence symptoms among the three groups showed that the scores of EA and L NAME groups were significantly lower than those of AS group at 24 hr, 48 hr and 72 hr after withdrawal of morphine injection ( P <0.01). In comparison with AS group, nNOS mRNA expression of EA group and L NAME group was lower clearly, suggesting a suppression of nNOS mRNA expression by EA. Conclusion: In morphine natural abstinence syndrome rats, acupuncture of ST 36 may down regulate nNOS mRNA expression in brain tissues.1128|0|2更新时间：2023-08-11
- 摘要：Objective:To explore the effect of acupuncture on the contents of hypothalamic neuropeptides in obesity rats. Methods: 15 SD rats were randomly and evenly divided into control, model and acupuncture groups. Obesity model was established before experiments. “Zusanli"(ST 36) and “Neiting"(ST 44) were punctured and stimulated electrically (10?Hz, 1.5?V, continuous waves) for 10?min , once daily, continuously for 14 times. Changes of body weight, Lee's index[3body weight (g)/ body length(cm)×10 3], body fat weight, as well as the contents of β endorphin (β EP), α melanocyte stimulating hormone (α MSH), bombesin (BOM), neuropeptide Y (NPY), angiotensin Ⅱ (AngⅡ), atrial natriuretic polypeptide (ANP) and calcitonin gene related peptide (CGRP) in the hypothalamic tissue of obesity rats were assayed with radioimmunoassay. Results: The body weight, Lee's index, body fat weight, and the levels of β EP, NPY and AngⅡ in hypothalamic tissue of obesity rats were significantly higher than those of normal rats ( P <0.05～ 0.01) , but the levels of α MSH, BOM, ANP and CGRP of obesity rats were significantly lower than those of normal rats ( P <0.05～0.01). There were positive interrelation between the contents of β EP, NPY, AngⅡ and body weight, obesity index and the body fat weight; but the interrelation between the contents of α MSH, BOM, ANP and CGRP and the obesity index was negative. After acupuncture treatment the marked weight lose effect was achieved while the contents of β EP, NPY, AngⅡ in hypothalamic tissue were markedly decreased ( P <0.05～0.01), and those of α MSH, BOM, ANP and CGRP in hypothalamic tissue obviously increased ( P <0.05～0.01). Conclusion: The abnormal increase of β EP, NPY and AngⅡ levels as well as the abnormal decrease of α MSH, BOM, ANP and CGRP levels in rat hypothalamic tissue may be responsible for obesity. Acupuncture induced changes of neuropeptide levels in the hypothalamic tissue of obesity rats possibly contribute to its antiobesity action in clinic.1101|0|5更新时间：2023-08-11
- 摘要：Objective:To elucidate the underlying neuroimmunological mechanism of the effect of EA in resisting inflammatory pain. Methods:21 Wistar rats were evenly randomized into control, inflammation and electroacupuncture (EA) groups. Focal inflammation was produced by subcutaneous injection of 5% formalin (50?μL) into the rats' hindpaw. EA (4～16?Hz, 1～3?V) was applied to “Huantiao”( GB 30) and “Yanglingquan” (GB 34) for 30?min. Spontaneous pain related response(flinching and lifting/licking the affected hindpaw), inflammatory reaction, the substance P (SP) and Interleukin (IL) 1β positive immunoreaction (IR) cells of the epidermis, dermis and hypodermis of the focal tissue were observed with behavioral measures and immunohistochemical techniques separately. Results: EA significantly reduced spontaneous pain related responses in focal inflammatory rats( P <0 01). The total number of inflammatory cells (per 100×100?μm 2 section) in EA group was significantly lower than that of inflammation group, suggesting an anti inflammatory effect. The integral optical density values of the SP IR and IL 1β IR positive cells in the focus were significantly higher than those of control group, whereas those of the two types of IR positive cells were considerably lower than those of inflammation group( P <0.01), suggesting that EA may resist inflammation and pain by suppressing the synthesis and release of SP from the focal sensory nerve endings, reducing the migration of immunocytes to the focus and lessening the synthesis and release of IL 1β.Conclusion: SP and IL 1β participate in the process of inflammatory pain induced by formalin, and EA may relieve focal inflammatory pain by reducing SP and IL 1β levels.1102|0|9更新时间：2023-08-11
- 摘要：Objective: To observe the effect of electroacupuncture (EA) on the inflammation and immune function in arthritis rats. Methods: A total of 32 Wistar rats were evenly randomized into control, model, EA GV 14 and EA GV 4 groups. Arthritis model was established by subcutaneous injection of Freund's complete adjuvant into the right hind paw (0.1 mL/animal). In EA GV 14 and EA GV 4 groups, “Dazhui” (GV 14) and “Mingmen” (GV 4) were punctured and stimulated electrically (15 Hz, 1 mA) for 20 min. The treatment was given once every other day, continuously for 8 times. The foot swelling rate[( foot volume after establishing arthritis model minus foot volume before establishing model) ×100%] was calculated. IL 2 activity of spleen and IL 2 concentrations of serum and brain tissues were assayed with MTT colorimetric analysis and radioimmunoassay separately. Results: After establishing arthritis model, the foot swelling rates of both hind limbs were significantly higher than those of normal control group ( P <0.01); and following EA of both GV 14 and GV 4, the swelling rates of the left and right feet in EA GV 14 and EA GV 4 groups both decreased considerably compared with those of model group ( P <0.01), suggesting a good anti inflammatory effect of EA. No significant difference was found between EA GV 14 and EA GV 4 groups ( P >0.05) in the foot swelling rate. In arthritis rats of model group, IL 2 activity of the spleen tissue declined significantly ( P <0.01), IL 2 level in the hypothalamus tissue increased significantly ( P <0.05), no significant changes were found in IL 2 levels of the serum and adrenal gland. Compared with model group, spleen IL 2 activity of the two EA groups increased significantly ( P <0.01), hypothalamic IL 2 contents of EA groups lowered to a certain degree. Conclusion: EA has an apparent anti inflammatory effect and its resultant changes of IL 2 activity of the spleen and IL 2 content in the hypothalamus may contribute to its regulatory effect on the immune function by way of the neuroendocrine network.1057|0|2更新时间：2023-08-11
- 摘要：To study the effects of moxibustion of Baihui(GV 20) on the hemodynamics of middle cerebral artery (MCA) and posterior cerebral artery (PCA) in health subjects.Methods: Thirty cases of healthy male students between 20 and 22 years in the age were enrolled into this study. Vmax (maximal velocity of blood flow),Vmin (minimal velocity of blood flow), PI (pulsatile index) and RI(resistant index) of right MCA and PCA were measured before and after moxibustion of GV 20 (5～10?min each time, once daily, 5 times altogether) by using Acuson Sequoia 512 Detector of Ultrasound Diagnostics.Results:Following moxibustion of GV 20, Vmax and Vmin of the right MCA, Vmax, Vmin,PI and RI of the right PCA increased significantly ( P <0.05～0 01). The rest indexes had no significant changes( P >0 05).Conclusion:Moxibustion of Baihui(GV 20) can significantly raise the velocity of blood flow of the right middle cerebral artery and posterior cerebral artery in normal people.1048|0|9更新时间：2023-08-11
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- 摘要：Objective:To observe the therapeutic effect of electroacupuncture (EA) in the treatment of ureterolith patients. Methods: 121 cases of ureterolith patients were randomly divided into EA group ( n =63) and medication group ( n =58). In EA group, Shenshu (BL 23), Pangguangshu (BL 28), Zhongji (CV 3), Weizhong (BL 40), Sanyinjiao (SP 6), Zusanli (ST 36), Neiguan (PC 6), etc. were punctured and stimulated electrically for 30?min. Patients of medication group were treated with intramuscular injection of Pethidine Hydroenloride(100?mg) and Anisodaminum (20 mg), once daily. Results: After two weeks' treatment, comparison between EA group and medication group in pain relieving time (16.33±6.37 min vs 15.44±6.01 min )and analgesia maintaining time (4.60±1.42 hr vs 4.54±1.40 hr ) showed no significant difference ( P >0.05), suggesting that both acupuncture and medication could remarkably relieve calculus induced ureteral colic. Results of ultrasonic examination or X ray film examination showed that the lithiasis removal rates of EA and medication groups were 60.32% and 43.10% respectively. Conclusion: Electroacupuncture can effectively promote the removal of ureteral calculus and reduce lithiasis induced colic in ureterolith patients.810|0|1更新时间：2023-08-11
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- 摘要：Currently, many hypothesizes about the mechanism of acupuncture therapy have been proposed by international scholars with different backgrounds. The present paper based on the knowledge of cytobiology and physiology mainly focuses on the relationship between acupuncture induced Deqi (achieving needling sensations) phenomenon and connective tissues. Also, it reviews the researches about the signaling transduction between cells and extracellular matrix (ECM) in the connective tissue. By doing so, we attempt to explain the relevance of the connective tissue to acupuncture and its effectiveness.1022|0|9更新时间：2023-08-11
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