最新刊期
卷 ， 期 1 ， 2005
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- 摘要：Objective: To observe the effect of Electroacupuncture (EA) of “Neiguan”(PC 6) on ATPase activity of capillaries in acute myocardial ischemia (AMI) rats. Methods: Sixty-six Wistar rats were randomized into normal (n=3), sham-operation (n=3), model and EA groups, and the last two groups were further divided into 2h, 2d, 1w, 3w subgroups respectively, with 6 cases being in each subgroup. AMI model was established by occlusion of the descending anterior branch (DAB) of the left coronary artery. EA (40 Hz, 4 V) was applied to “Neiguan”(PC 6) for 5 min, once daily till sampling (2h, 2d, 1w, 3w). The reaction products of Mg 2+ -ATPase and Na+-K+-ATPase of the left cardiac ventricle and apical region tissues (cut into sections, 10 μm in the thickness) were detected by using enzyme histochemical staining and enzyme cytochemical staining techniques and observed under electronic microscope, and the optic density (OD) or grey scale (GS) values of the reaction products of the tissue sections were detected with a multimedia image analysis system. Results: Comparison among different groups showed that the total area values of Mg 2+ -ATPase and Na+-K+-ATPase reaction products of the vascular endothelium in the myocardial tissue of model group from 2h to 3w were significantly lower than those of normal group (P<0.05～0.01), those of EA group from 2h to 3w were significantly higher than those of model group (P<0.05～0.01). Regarding the average OD/GS values, compared with normal group, in model group, GS of Mg 2+ -ATPase reaction products increased significantly but OD of Na+-K+-ATPase reaction products lowered markedly (P<0.05～0.01); compared with model group, in EA group, GS of Mg 2+ -ATPase reaction products at different time courses decreased significantly further, while OD of Na+-K+-ATPase reaction products increased remarkably at all the time courses except 2w (P<0.05～ 0.01). In regard to the number of capillaries, those of model group from 2h to 3w were considerably fewer than that of normal group (P<0.01); compared with model group, those of EA group from 2 h to 2w were considerably more (P<0.05～0.01). No significant differences were found between normal and sham-operation groups in these indexes. Microscopic observation displayed that after AMI, the state of myocardial fiber fracture, edema, degeneration, necrosis, and the vague outline of micrangium in model group was severer, while that in EA group was lighter. Conclusion: EA of Neiguan (PC 6) has effects of improving ATPase activity in endothelial cells of the ischemic myocardial micrangium and protecting anoxic myocardial cells.1112|0|3更新时间：2023-08-11
- 摘要：Objective: To observe the effect of electroacupuncture (EA) on the expression of NPY and its mRNA in hypothalamus of streptozotocin (STZ)-induced diabetes mellitus (DM) rats. Methods: Forty-three Wistar rats (180～220 g) were assigned to normal control group (n=13), model group (n=11) and EA group (n=11). DM model was established by intraperitoneal injection of STZ(50 mg/kg). EA (100 Hz, 0.5～2 mA, 15 min) was applied to bilateral “Yishu”,“Zusanli”(ST 36) and “Guanyuan”(CV 4), once daily and with 6 sessions being a therapeutic course. After two courses of treatment, the rats were sacrificed to test the expression of NPY and its mRNA in hypothalamus with immunohistochemical and in situ hybridization methods respectively. Results: Compared with normal group (n=5), OD values of NPY expression positive fibers in hypothalamic paraventricular nucleus (PVN) and arcuate nucleus (ARC), the OD value and the total area of NPYmRNA expression in lateral hypothalamus (LH) increased significantly (P<0.05) in DM rats of model group (n=5). Comparison between model group and EA group (n=5) showed that OD values of NPY expression positive fibers in PVN and ARC, the OD value and the total area of NPYmRNA expression in LH of the later group were significantly lower than those of the former group (P<0.05), displaying that after EA, the concentration of NPY and expression of its mRNA in hypothalamus decreased obviously. Conclusion: EA can reduce the level of NPY and its synthesis in hypothalamus of STZ-induced diabetic rats, which may contribute to its effect for improving energy metabolism of diabetes.1091|0|3更新时间：2023-08-11
- 摘要：Objective: To study a suitable animal model for analyzing the therapeutic effect and mechanism of electroacupuncture (EA) in the treatment of depression. Methods: For 1) norepinephrine (NE) toxic test, 2) 5-hydroxytryptophan (5-HTP) head-twitch test and 3) tail-suspension test: three series of 30 male Kunming mice were respectively randomized into control, Prozac-20 and EA groups with 10 cases being in each group. Rats of Prozac-20 group were fed with Prozac-20 (0.2 mL/10 g, 2.6 mg/kg), once daily for one week; those of EA group were given with EA (2 Hz, 0.2～0.3 mA for mice, 0.6mA for rats) of “Baihui”(GV 20) and “Yintang”(EX-HN 3), once daily for one week. NE (1.2 mg/mL, 12 mg/kg, subcutaneous injection) for observing NE toxicity test, hydrochloric pargylin (100 mg/kg, i.p.) and 5-HTP (10 mg/kg, i.v) for head-twitch were given respectively to the rats of various groups on the 7th day. In another part of experiments, chronic stress model was established by forced ice-water swimming, tail-clamping, electric-shock stimulation, etc. 50 SD rats were equally randomized into control, model, Prozac-20 and bounding groups and EA groups. The methods for EA were the same to those mentioned above. The open field test for recording the rats' rearing and crossing motion times and the sucrose-intake volume were calculated separately. Results: Compared with control group, NE-induced death rate and 5-HTP-induced head-twitch times of EA group had no significant changes; but the immobility duration for tail-suspension test of EA group was significantly lower than those of both control and Prozac-20 groups (P<0.05). In chronic stress rats, the crossing and rearing motion times decreased considerably in comparison with those of control group, while in comparison with rearing motion times of model group, those of both Prozac-20 and EA groups increased significantly (P< 0.05), and those two indexes of EA group also significantly higher than those of bounding group (P<0.05). Compared with control group, the sucrose-intake volume of model group and bounding group decreased significantly (P<0.05); while compared with bounding group, the sucrose-intake volume of EA group increased significantly (P<0.05), and no significant difference was found between prozac-20 and EA groups in the volume. Results showed that EA could improve mice's and rats' behavior responses (shortening mice's duration of tail-suspension immobility, and increasing stress rats' locomotor activity). Conclusion: Drug-induced acute depression animal model is not suitable to be used for researching mechanisms of acupuncture, thus, it is recommended to conduct acupuncture research in the changed animals' living conditions and environment or in chronic depression animals.1012|0|17更新时间：2023-08-11
- 摘要：Objective: To investigate the effect of acupuncture on the memory and SOD activity in the cerebral issues in Alzheimer's disease (AD) rats. Methods: 48 healthy Wister rats were randomly divided into control group (n=11), model group (n=10), medication group (n=10) and acupuncture group (n=11). AD model was established by microinjection of β-amyloid protein (β-AP)1-40 (1 μL) into the hippocampal CA1 region (AP: 2.22 mm; ML: 3.0 mm, DV: 2.8 mm). In acupuncture group, “Baihui”(GV 20) and “Sishencong”(EX-HN 1) were punctured with filiform needles which were lifted, thrust and twirled continuously for 5 min, and then retained for 20 min. The treatment was conducted once daily except Sundays and continuously for one month. Rats of medication group were fed with Piracetum (40 mg/mL, 6 mL/kg/time) once daily for 30 days. The rats' memory was detected with shuttle-box test and the activity of superoxide dismutase (SOD) in the cerebral issues assayed by enzymological method. Results: Shuttle box test showed that compared with control group, the times and duration of electric shock were significantly more and longer than those of model group (P<0.05); while compared with model group, the times and duration of electric shock of both acupuncture and medication groups were significantly more and longer (P<0.01). No significant differences were found between two groups in these two indexes, suggesting that both acupuncture and medication can improve AD rats' memory. In comparison with control group, SOD activity in the cerebral tissue lowered considerably; while in comparison with model group, SOD activity of both medication and acupuncture groups increased significantly (P<0.01), and no significant difference was found between the later two groups (P>0.05). Conclusion: Acupuncture treatment can improve AD rats' learning and memory and raise SOD activity in the cerebral tissue.1110|0|4更新时间：2023-08-11
- 摘要：Objective:To observe the effects of electroacupuncture (EA) at different time on somatostatin (SOM)mRNA expression in the spinal ganglia and spinal cord in rats. Methods: A total of 40 SD rats were evenly randomized into EA and control groups which were further divided into 23-o'clock (Zi Shi), 5-o'clock (Mao Shi), 11-o'clock (Wu Shi) and 17-o'clock (You Shi) subgroups respectively, with 5 cases being in each subgroup. EA (3 V, 2 Hz, wave width 1 ms) was applied to unilateral “Huantiao”(GB 30) for 30 min. After acupuncture, these rats anesthetized with urethane were infused with 1% paraformaldehyde, 1.25% glutaraldehyde plus 0.1 mol/L HCl buffer solution. Then, L_5 segment of the spinal cord and dorsal root ganglia were taken out to be cut into sections (25 μm). SOMmRNA expression was detected by using in situ hybridization method. Results: After EA of “Huantiao”(GB 30), the number of the densely-stained SOMmRNA positive neurons in the spinal ganglia and laminaⅠandⅡ of the posterior horn of the spinal cord increased markedly at 11-o'clock compared with that of control group at the same time course (P<0.05), but no significant differences were found between two groups at the other time courses. Conclusion: After EA, the expression of SOMmRNA positive neurons in the spinal ganglia and spinal cord is upregulated at 11.00 in rats.1114|0|4更新时间：2023-08-11
- 摘要：Objective:To observe the effect of acupoint application on gastric mucosal blood flow, prostaglandin E_2 content in rats with chronic atrophic gastritis (CAG). Methods:A total of 48 SD rats were evenly randomized into control group, model group, acupoint application “Zusanli” (ST 36) and “Zhongwan”(CV12) group and medication group. CAG model was established by feeding the rat with 0.02% Ammonia solution for 16 weeks. Rats of medication group were fed with “Weisu Chongji” (herbal granule for protecting gastric function, 804 mg/200 g body weight). Acupoint application and gastric perfusion of “Weisu Chongji” were given once daily, continuously for 2 months. Gastric mucosal blood flow (GMBF) and prostaglandin E_2 content (PGE_2) of the gastric mucosal tissue were assayed with neutral red clearance method and radioimmunoassay respectively. Results: In comparison with control group, the rats' body weight, GMBF and gastric mucosal PGE_2 levels of model group were significantly lower (P<0.01); while the body weight and GMBF of acupoint application and medication groups, and gastric mucosal PGE_2 content of acupoint application group were significantly higher than those of model group (P<0.01). Comparison between acupoint application and medication groups showed that the body weight, GMBF and gastric mucosal PGE_2 content of acupoint application group were significantly higher than those of medication group (P<0.05). The facts indicated that acupoint application and medication both could improve gastric mucosal blood flow and CAG rats′ constitution and raise gastric mucosal PGE_2 level and the therapeutic effect of acupoint application was superior or significantly superior to that of medication. Conclusion:Acupoint application can protect gastric mucosa by improving GMBF and increasing PGE_2 in chronic atrophic gastritis rats and is thus one of the effective therapies for CAG in clinic.1028|0|3更新时间：2023-08-11
- 摘要：Objective: To observe the relationship among Nucleus of the Solitary Tract (NTS), “Sibai” (ST 2) and stomach in the rat. Methods: Thirty-two SD rats were randomized into normal control group, “Sibai” group, non-acupoint (0.5 cm beside ST-2) group and gastric-distention group with 8 cases being in each group. EA (4 Hz, 50 Hz, 20 V) was applied to “Sibai” (ST 2) and non-acupoint for 20 min. Gastric distention was conducted for 20 min by using a balloon filled with 5 mL of normal saline. The sections of medulla oblongata tissues containing NTS were stained with immunohistochemical technique (ABC method) for displaying expression of c-fos. Results: Microscopic observation showed that fos-like immunoreaction (IR) positive cells mainly presented in medial subnucleus of NTS (mnTS), middle and caudal medulla oblongata. The IR-positive cells of mnTS in normal control, “Sibai”, non-acupoint and gastric-distention groups were 5.50 ±2.30, 32.03±7.35, 14.76±3.06 and 31.03±3.60 respectively. The IR-positive cells of “Sibai”, non-acupoint and gastric-distention groups were significantly more than those of normal control group (P<0.01), those of “Sibai” group were significantly more than those of non-acupoint group; and no significant difference was found between “Sibai” and gastric distention groups (P>0.05). Conclusion: Results display that the sensatory information from EA of “Sibai” (ST 2) and gastric distention may converge on NTS, suggesting a relatively specific connection between Sibai and stomach.1122|0|4更新时间：2023-08-11
- 摘要：Objective:To observe the distribution of the activated cerebral regions after acupuncture stimulation of Shenmai (BL 62) by using functional magnetic resonance imaging (fMRI) technique. Methods: A total of 6 cases of volunteer healthy subjects were recruited for this study. After insertion, the acupuncture needle was twirled about 180° and at a frequency of about 120 times/min for 5 min, and then, retained to the end of each experiment. Before and during manipulation and retention of the needle, cerebral images were sampled separately using a superconductor magnetic resonance scanner and analyzed by using SPM software. Results:During manipulation of the needle inserted in the unilateral Shenmai (BL 62), the highly-activated cerebral structures were limbic system(thalamus, cingulum gyrus, parahippocampal gyrus), middle frontal gyrus, posterior lobe of the cerebellum, etc., on the contralateral side of the brain; interthalamic adhesion, habenular commissure of habenulae, white matter, occipital lobe, etc.; bilateral superior temporal gyrus. During retention of the needle, the highly-activated cerebral structures were bilateral parahippocampal gyrus, dorsal thalamus, cingulum gyrus, superior frontal gyrus, etc.; ipsilateral temporal lobe, amydaloid nucleus, occipital lobe, etc. Five minutes later (from the beginning of retention of the needle), the activated degree of the aforementioned cerebral structures such as parahippocampal gyrus, bilateral posterior lobe of the cerebellum, etc, declined clearly. These results provide spatial and temporal information about the neural activity of different regions of the brain during acupuncture of BL-62 and retention of the needle. Conclusion: The aforementioned results show that some cerebral regions are activated by acupuncture stimulation of Shenmai (BL 62) and may be involved in the therapeutic effect of acupuncture of this acupoint in the treatment of some related diseases.773|0|8更新时间：2023-08-11
- 摘要：Objective: To study the effective curative methods for cervical vertigo. Methods: A total of 114 vertigo patients were randomly divided into acupuncture group (64 cases) and medication group (50 cases). In acupuncture group, the main points Naokong (BL 19) to Fengchi (GB 20), Yuzhen (BL 9) to Tianzhu (BL 10), Sizhukong (TE 23) to Shuaigu (GB 8), Hanyan (GB 4) to Xuanlu (GB 5), etc were used. Patients of medication group were treated with intravenous drip of Danshen (red sage root) Injection and oral administration of Betahistine Hydrochloride tablet (5 mg, once/d) for 30 days. Results: After 3 courses of treatment, of the 64 and 50 cases in acupuncture and medication groups, 12 (18.75%) and 8 (16.0%) cases were cured, 47 (73.44%) and 31 (62.00%) experienced improvement, 5 (7.81%) and 11 (22.00%) failed, with the effective rates being 92.19% and 78.00% respectively. The therapeutic effect of acupuncture group was significantly better than that of medication group (P<0.05). Conclusion: The therapeutic effect of acupuncture therapy is superior to that of medication in the treatment of cervical vertigo.1003|0|0更新时间：2023-08-11
- 摘要：Objective:To compare clinical therapeutic effects of acupuncture therapy and medication for stable angina pectoris. Methods:52 cases of coronary heart disease (CHD) patients with stable angina pectoris were randomly divided into acupuncture group (n=30) and medication group (n=22). The acupoint groups used were 1) Xinshu (BL 15) and Jueyinshu (BL 14); 2) Juque (CV 14) and Shanzhong (CV 17), and the adjunct acupoints were bilateral Neiguan (PC 6), Ximen (PC 4), Shenmen (HT 7) and Geshu (BL 17). Acupuncture treatment was given once daily, 2 weeks altogether. For patients of medication group, Isosorbide Dinitrate (10 mg, t.i.d.) or Metoprolol (12.5 mg/d) and Nitroglycerin (when necessary) were given for 2 weeks. Results:In regard to the improvement of patients' symptoms in the frequency and duration of angina attack and severity of pain, the markedly effective, effective and failure cases in acupuncture and medication groups were 14 and 12, 13 and 6, 3 and 4 cases respectively, with the total effective rates being 90.00% and 81.82% separately, and the curative effect of acupuncture group being markedly superior to that of medication group (P<0.05). No significant differences were found between two groups in changes of ECG-ST and T waves. During treatment, the rates for cease and reduction of Nitroglycerin-taking in acupuncture and medication groups were 23.3% (11/30) and 18.2% (7/22) respectively. Conclusion:Acupuncture therapy is effective in relieving CHD patients' angina pectoris.890|0|4更新时间：2023-08-11
- 摘要：This article puts forward the concept of syndromes appeared in the ancient Chinese literature about acu-moxibustion, and the ways and measures (including software and database) for identifying various syndromes so as to make a quantitative analysis on the results of methodology of traditional literature research from multiple angles and different layers and to explore the methods, significance and tactics of computer applied techniques in the course of this study. Thus, it can be considered as a preliminary attempt to realize computerized statistical analysis about diseases, syndromes and symptoms described in classical medical works.1005|0|0更新时间：2023-08-11
- 摘要：In the present paper, the authors review development of researches on electroacupuncture (EA) treatment of depression from 1) clinical therapeutic effect: a) comparison between EA and antidepressant, b) comparison between EA combined with antidepressant and simple antidepressant, c) comparison between EA combined with antidepressant and simple EA, d) comparison between EA and filiform needle; 2) researches on the underlying mechanisms of EA in the treatment of depression: a) clinical studies, and b) experimental studies. Clinical studies indicate that EA may improve depression patients' immune function and regulate levels of T cells, activity of IL-2, IL-1β, IL-6, TNF-α, etc. and experimental researches show that monoamines neurotransmitters and their receptors, hypothalamus-pituitary anterior lobe (HPA)-axis, brain-derived neurotrophic factor, etc are involved in the effect of EA in improving depression.1100|0|7更新时间：2023-08-11
- 摘要：In the present paper, the authors review recent progress about the study on the underlying mechanism of acupuncture and moxibustion in protecting gastric mucosal tissues from lesion from 1) neuro-regulation, 2) gastrointestinal hormones, 3) gastric mucosal blood flow, 4) body fluid factors, 5) oxygen-derived free radicals, 6) stomach wall barrier, etc. Moreover, the authors present some existed problems (such as clinical preventive treatment, observation on the long-term effect, fewer studies on the effect of acupuncture from cellular and molecular levels, etc.) in current scientific research about acupuncture and moxibustion, and suggest that future studies should focus on the comprehensive analysis about the mechanism of acupuncture in protecting the gastric tissue from lesion from the wholism, intracellular information transmission, molecular level, etc.1125|0|5更新时间：2023-08-11
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