最新刊期
卷 ， 期 2 ， 2005
- 摘要：Objective: To investigate the effect of electroacupuncture (EA) on brain injury in rats with cerebral ischemia-reperfusion (CI-R). Methods: A total of 104 SD rats were randomized into sham-operation (n=8), model-Ⅰ (CI-R-24 h, n=16), EA-Ⅰ (n=16), medication (melatonin, 3.2 mg/kg, i.p. n=8; Red Sage Injection, 0.3 g/kg, i.v., n=8), EA+medication (n=8), EA-prevention (n=8), model-Ⅱ(CI-R-120 h, n=8) and EA-Ⅱ(n=8) groups. CI-R model was established by right middle cerebral artery occlusion (MCAO) for 60 min and reperfusion for 24 hours or 120 hours. EA (3 Hz, 2～4 mA, continuous waves) was applied to “Dazhui” (GV 14) and “Baihui” (GV 20) for 30 min. Glutathione peroxidase (GSH-Px) and catalase (CAT) activity,and malondialdehyde (MDA) contents of the serum and hippocampus, striate body and cerebral cortex tissues were detected separately by biochemical methods. Results: Compared with sham-operation group, the activity of GSH-Px of serum and the aforementioned 3 cerebral regions of model-Ⅰ and model-Ⅱ groups decreased significantly (P<0.01), while MDA contents of these two model groups increased considerably (P<0.01). In comparison with model-Ⅰ group, GSH-Px activity of serum and the 3 cerebral regions of EA-Ⅰ group increased significantly (P<0.05～0.01), while MDA levels of EA-Ⅰ group lowered significantly (P<0.05). The effects of EA+red sage injection in raising GSH-Px activity and lowering MDA levels of both serum and brain tissue were markedly superior to those of EA-Ⅰ group (P<0.05～ 0.01). No significant differences were found between EA-Ⅰ and medication groups in these two indexes ( P> 0.05). Compared with model-Ⅱ group, GSH-Px activity of EA-Ⅱ in serum and the 3 cerebral regions increased significantly (P<0.05～0.01), while their MDA contents decreased significantly ( P< 0.05～ 0.01). Conclusion: EA of GV-14 and GV-20 can prevent or inhibit CI-R-induced decrease of GSH-Px activity and increase of MDA in both blood and cerebral tissues, suggesting a good effect of EA in resisting lipid peroxidation reaction and oxygen free radical injury of CI-R, i.e., resisting oxygen stress and protecting the brain from CI-R damage.1023|0|6更新时间：2023-08-11
- 摘要：Objective: To investigate the mechanism of the effects of different stimulus intensities of electroacupuncture (EA) at the same acupoint. Methods: 11 adult Wistar rats were divided into control group (n=3), moderate EA group (n=4) and strong EA group (n=4). EA (moderate intensity: 2～4 mA, 20/ 4 Hz; high intensity: 5～7 mA, 20/4 Hz) was applied to “Yanglingquan”(GB 34) and “Zusanli”(ST 36) for 60 min, once every other day, 3 times altogether. On the 7 th day, these rats were decapitated for taking brain tissue which was then cut into sections (10 μm) to be mounted to glass slides. The expression of phospho-Akt in hippocampus (CA1), dentate gyrus and cerebral cortex of the rats was examined by immunohistochemistry and Western blot analysis. Results: Immunohistochemical results showed that the p-Akt expression-positive neurons in CA1, dentate gyrus and cerebral cortex of moderate EA group were significantly more than those of strong EA and control groups (P<0.01). P-Akt positive neurons of the cerebral cortex in strong EA group were significantly more than those of control group (P<0.05), but no marked differences were found between strong EA and control groups in p-Akt expression positive neurons in CA1 and dentate gyrus (P> 0.05). Results of Western blot were similar to those of immunohisochemical staining. Conclusion: Moderate intensity EA is superior to strong EA in promoting the expression of p-Akt in rat hippocampal CA1, dentate gyrus and cerebral cortex.1030|0|6更新时间：2023-08-11
- 摘要：Objective: To study the mechanism of acupuncture in reducing body weight in hypothalamic obesity rats. Methods: 80 SD rats were randomly and evenly divided into normal control, model, acupuncture and medication groups. Obesity model was established by subcutaneous injection of 15% sodium glutamate solution (0.2 mL/10 g), once daily and continuously for 5 days. Lee’s index, body weight and length, serum total cholesterol (TCh), triglyceride (TG), high density lipoprotein-cholesterol (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch) were detected with routine biochemical techniques, and serum insulin, leptin and neuropeptide Y contents assayed with radioimmunoassay. EA (dense-sparse waves, frequency of 100 Hz, a suitable strength inducing local muscular tremor) was applied to bilateral “Zusanli” (ST 36)-“Sanyinjiao” (SP 6), and “Guanyuan” (CV 4)-“Zhongwan”(CV 12) for 15 min, once daily and continuously for 28 days. Rats of medication group were fed with Sibutramine (4 mg/kg/d, 28 days altogether). Results: Compared with normal control group, Lee’s index, wet weight of the left perirenal adipose tissue, the area of the lipocytes, serum TG, TCh and LDL-Ch, insulin, leptin and NPY contents and NYP content of the hypothalamus tissue of model group increased significantly (P<0.01); whereas, lipocyte number, serum HDL-Ch and lipoprotein lipase (LPL) and hypothalamic leptin contents of model group decreased significantly (P< 0.01). In comparison with model group, body weight, Lee’s index, wet weight of the left perirenal adipose tissue, area of the lipocytes, serum TG, TCh and LDL-Ch, insulin, leptin and NPY and hypothalamic NPY contents of acupuncture and medication groups lowered considerably (P<0.05～0.01), while the number of lipocytes, serum HDL-Ch and lipoprotein lipase (LPL) and hypothalamic leptin contents of acupuncture and medication groups increased significantly (P<0.05～ 0.01). Comparison between acupuncture and medication groups showed that Lee’s index, serum TG, insulin, leptin and NPY and hypothalamic NPY contents of acupuncture group were significantly lower than those of medication group (P<0.05～0.01), while the number of lipocytes, serum LPL and hypothalamic leptin contents of the former group were significantly higher than those of the later group (P<0.05～0.01). Conclusion: Acupuncture has a good effect in reducing body weight by improving lipoprotein metabolism and regulating blood insulin, blood and hypothalamic leptin and NPY secretion.1154|0|2更新时间：2023-08-11
- 摘要：Objective:To investigate changes of blood lipid in ovariectomy rats after treatment with acupuncture and moxibustion in advance. Methods:Forty SD female rats(3.5 month old) were randomly and evenly divided into sham-operation group,ovariectomy group,preventive acupuncture group and preventive moxibustion group. In preventive acupuncture and preventive moxibustion groups, “Guanyuan” (CV 4) was punctured with the needle retained for 20 min, and given with moxibustion for one moxa-cone respectively. The treatment was conducted twice every week, 4 weeks altogether. One month later,all the rats were killed for collecting blood examples. Serum total cholesterol (TCh),triglyceride (TG),high density lipoprotein (HDL) and low density lipoprotein (LDL) concentrations were detected with colorimetry. Results:Compared with sham-operation group, TCh and TG of ovariectomy group increased slightly(P> 0.05), but HDL decreased significantly and LDL increased significantly(P<0.05), showing an increase of blood lipid in ovariectomized rats. Compared with ovariectomy group,TG levels of preventive acupuncture group and preventive moxibustion group decreased significantly(P<0.05),and HDL levels of these two groups increased significantly(P<0.01). In addition,the level of LDL of preventive moxibustion group decreased significantly compared with ovariectomy group(P<0.05). Besides,there were no considerable differences in the levels of the 4 indexes of blood lipid among preventive acupuncture,preventive moxibustion and sham-operation groups(P>0.05). Conclusion: Preventive acupuncture and preventive moxibustion can play a certain role in resisting or adjusting disorders of blood lipid in the ovariectomized rats.997|0|1更新时间：2023-08-11
- 摘要：Objective: To prove the priority of music electroacupuncture (EA) in inducing analgesia by comparing its effect with that of pulse EA from behavior and neuroelectrophysiology. Methods: 120 Wistar rats anesthetized with 5% urethane + 0.5% chloralose (i.p., 10 mL/kg) were randomized into behavior part and neuronal reaction observation which were subdivided into control group, music-EA group and pulse-EA group respectively with 20 cases in each group. Pain reaction to radiation-heat irradiation was observed by using tail flick latency (TFL) test. Electric pulse EA and music (music sound waves) EA (6 Hz, 2 V and a suitable strength able to induce local muscular tremor) were applied to bilateral “Zusanli”(ST 36) and “Sanyinjiao”(SP 6) for 30 min/time, 6 times altogether, followed by detecting TFL. Extracellular discharges of neurons of the left caudate nucleus (CN) and the right hippocampus (HIP) were recorded by using glass micropipettes. Reactions of neuronal firing to electric stimulation of sciatic nerve (5 mA, duration of waves 0.3 ms, interval 5 ms, 5 pulses/train) or EA were fed to Power-Lab/8s data processing system. Results: In comparison with control group and pre-EA, TFL values of pulse EA from the 1 st to 3 rd time of EA, and those of music EA from the 1 st to 6 th time of EA delayed significantly (P<0.05～0.01). TFL values of music EA group were significantly bigger than those of pulse EA and control groups from the 1 st to 6 th time of EA (P< 0.05～0.01 ). Compared with the latency values of pain excitement reaction of firings of CN and HIP neurons of control group, those of both pulse EA and music EA groups increased significantly (P<0.05), while the net increase values of the firing frequency of the two EA groups were markedly smaller (P<0.05～0.01); and the effects of music EA were significantly better than those of pulse EA from the 1 st time of EA on (P< 0.05～0.01 ). The effects of music EA and pulse EA on pain inhibitory reaction of neuronal firings were similar to those of pain excitement neurons. Results showed that the analgesic effect of music EA was significantly superior to that of pulse EA and the analgesic effect of pulse EA was weakened gradually after multiple times of EA stimulation. Conclusion: Music EA has a better analgesic effect and has no marked tolerance phenomenon which may favor functions of acupoint stimulation.892|0|5更新时间：2023-08-11
- 摘要：Objective: To observe the effect of hyaluronidase on neuropathic pain in chronic constriction nerve injury (CCI) rats and analyze the possible mechanisms of acupoint-injection of hyaluronidase (a typical enzyme for decomposing extra-cellular matrical polysaccharide) for analgesia. Methods: A total of adult SD rats were randomly divided into control group (n=10) and treatment group (n=12). CCI pain model was established by ligating the left sciatic nerve loosely with gauge-4 chromic catgut. Silver chloride electrode was used to record the ectopic spontaneous discharges of the proximal filaments of the injured nerve 2 cm above the ligated site and the latency of foot withdrawal reaction to heat stimulation was detected with hot-plate test. After CCI, the injured sciatic nerve was taken out and cut into sections which were stained with periodic acid Schiff (PAS)-hematoxylin and eosin (HE) techniques. Results: In our experiments, the rate of ectopic spontaneous discharges of the nerve filament was significantly reduced by 50.9%±12.4% in 8 of the 12 CCI rats after applying hyaluronidase to the injured site but was not affected by application of normal saline or mechanical stimulation, suggesting an injured nerve discharge. Behavioral test for neuropathic pain indicated that heat hyperalgesia of CCI rats could be reduced by applying hyaluronidase to the CCI site. After CCI, the pain reaction (foot withdrawal) latency was delayed significantly. The difference values of the latency of foot withdrawal reaction between post- and pre-CCI on the 3 rd , 6 th and 14 th day in experimental group were lower or significantly lower (14 th day, P<0.05) than those of control group, indicating that local application of hyaluronidase could prevent the development of heat hyperalgesia. Histological observation displayed disordered arrangement of nerve fibers with bigger irregular spaces among them,swelling of tissues, deposition of polysaccharide granules and abundant cellular neuclei among the fibers, indicating appearance of inflammation in the injured portion of the sciatic nerve. In hyaluronidase treated samples, no deposition of protein polysaccharide granules was found, the arrangement of nerve fibers and cellular nuclei were basically normal. Conclusion: Extra-cellular matrix has a close relation with neuro-physiological functions and hyaluronidase can effectively suppress CCI induced ectopic spontaneous discharges. It suggests that clinical application of acupoint-injection of hyaluronidase to change local extra-cellular matrix may have an analgesic effect on chronic pain.849|0|0更新时间：2023-08-11
- 摘要：Objective: To investigate the effect of electroacupuncture (EA) on thymus and spleen and apoptosis of thymocytes in morphine withdrawal rats. Methods: Rat abstinence syndrome model was established by continuous muscular injection of morphine (with the injected doses increasing gradually from 4 mg to 140 mg/kg, 1～3 times/d) to the hind limbs for 15 days. A total of 24 Wistar rats were randomly and evenly assigned to normal control group, abstinence syndrome (AS) group and EA (ST-36) group. The net weight of the thymus and spleen were detected and recorded.TUNEL method was applied to mark the apoptosis of thymocytes. EA (2～100 Hz, 1～2 mA) was applied to bilateral “Zusanli”(ST 36) for 30 min, once daily, continuously for 7days. Results: In comparison with normal control group, a significant decrease of the weight of both thymus and spleen, and the thymus index and spleen index was observed in AS group(P<0.01). Comparison between AS group and EA group showed that the weight of both thymus and spleen of EA group increased significantly than that of AS group, and both thymus index and spleen index of EA group were bigger than those of AS group (P<0.01), indicating that the effect of AS was reversed by EA. TUNEL method showed that the apoptosis rates of thymocytes in normal control, AS and EA groups were 5.7%, 51.1% and 31.8% respectively, displaying that EA could suppress AS-induced apoptosis of thymocytes. Conclusion: EA of ST-36 may protect the immune organ and inhibit the apoptosis of thymocytes, which may account, at least partly, for the suppression of EA on immune organ impaired by morphine.1110|0|5更新时间：2023-08-11
- 摘要：Objective: To investigate the morphological relationship between cardiogenic referred pain and Heart Meridian of Traditional Chinese Medicine. Methods: A total of 11 SD rats anesthetized with 10% urethane (1 g/kg) were used in the present study. The fluorescent traces, fast blue (2～5%), propidium iodide (2～5%) and bizbenzimide (2～5%) were injected into heart and the acupoints “Shaohai” (HT 3) and “Qingling” (HT 2) of the Heart Meridian and “Xiabai” (LU 4) and “Chize” (LU 5) of the Lung Meridian respectively, with 5 μL in each acupoint. The labeled cells were observed in dorsal root ganglia (DRG) from C 6 to T 5 segments. Results: After injection of the abovementioned three fluorescent traces into the heart and the acupoints of the Heart Meridian and Lung Meridian of the left forelimb the ratios of the single-labeled cells over the total-labeled neurons in DRG of the C 6～T 5 segments were 29.07%, 24.03% and 24.42% respectively; the ratios of the double-labeled neurons over the total-labeled neurons were 12.14% and 7.5% following injection of the traces into the heart and the acupoints on the left Heart Meridian separately; and the ratio of triple-labeled cells over the total-labeled neurons was 1.16% after injection of the traces into the heart and the acupoints on the left Heart Meridian and Lung Meridian respectively. After injection of the three fluorescent traces into the acupoints with the same names as those mentioned above on the right forelimb the ratios of single-labeled over the total-labeled neurons were 26.98%, 26.89% and 21.97% respectively; the ratios of the double-labeled/total-labeled neurons after injection of the traces into the heart and right Heart Meridian, and the heart and right Lung Meridian were 10.81% and 9.58% respectively; and the ratio of the triple-labeled neurons to the total-labeled neurons following injection of the traces into the heart and the acupoints of the right Heart Meridian and the right Lung Meridian was 2.02%. Conclusion: The present study provides direct evidence showing the existence of dichotomized peripheral afferents that supply both visceral and somatic structures. The relationship between the Heart Meridian and the heart organ is closer than that between the Lung Meridian and the heart.1113|0|11更新时间：2023-08-11
- 摘要：Objective: To research the mechanism of moxibustion-induced high-temperature lines along the meridian from both structure and function. Methods: 16 adult Japanese big-ear white rabbits were used in the present study. Following shearing hairs at the lateral side of the lower limb, moxibustion was applied to bilateral “Zusanli”(ST 36) , 5～6 minutes later, thermogram along the “Stomach Meridian” was taken by infrared ray thermal instrument. The part of the lower limb showing higher-temperature band monitored by thermogramer was frozen and removed rapidly, the sample including skin, subcutaneous tissue and muscle was then cut into sections (10 μm or 20 μm) stained with histochemical techniques [glyoxylic acid (SPG)] for displaying acetylcholine esterase (AChE) and lactic dehydrogenase (LDH) activities. Results: After moxibustion of “Zusanli”(ST 36), higher temperature traces appeared alone the running course of the Stomach Meridian. Under light microscope, the bright green fluorescence intensity in the tissue sample of higher-temperature band (meridian course) was weaker, while that of extra-meridian tissue samples stronger. The optic density (OD) values of AChE and LDH in the slices of the higher-temperature tissues (along the Stomach Meridian) and non-higher-temperature tissues (extra-meridian) were 0.617±0.265 vs 0.514±0.169, and 0.265± 0.08 vs 0.199±0.05 (each n=32) respectively, displaying significant differences between meridian tissue and extra-meridian tissue in the OD values (P<0.01). It suggests that under higher-temperature state of the running course of meridian, the level of monoamines (adrenergic transmitter being predominant) was lower, and the activity of AChE and LDH higher in the tissues along the meridian than that in the tissues beyond the meridian (P<0.01). Conclusion: Moxibustion induced high temperature line reaction alone the Stomach Meridian is related to the decrease of monoamines (adrenergic transmitter) and the increase of AChE level and potentiation of energy metabolism.1056|0|6更新时间：2023-08-11
- 摘要：Objective: To observe the effect of acupuncture in strengthening the spleen to resolve phlegm and to study its possible mechanism in the treatment of stroke. Methods: 60 stroke(wind-phlegm blocking collateral type)patients were randomly and equally divided into control group and treatment group. Patients in treatment group were treated with principle of strengthening the spleen to resolve phlegm combined with needling methods for dispelling wind and dredging collaterals. Patients in control group were treated with principles of dispelling wind and dredging collaterals only. Main acupoits used were Baihui(GV 20), Shenting (GV 24), Fengchi (GB 20), Fenglong (ST 40), etc. The treatment was conducted once daily except Saturdays and Sundays,30 times altogether. Serum total cholesterol (TCh), triglyceride (TG), high density lipoprotein (HDL-Ch), apolipoprotein (Apo)-A and -B contents were detected according to the instructions of reagent kits. Results: After treatment, the total effective rates of treatment and control groups were 86.67% and 73.33% respectively, and the therapeutic effect of treatment group was significantly higher than that of control group (P<0.05). The score for wind-phlegm syndrome of treatment group was significantly lowered than that of control group (P<0.05). After treatment,serum TCh and Apo-B of treatment group decreased considerably, which was significantly lower than those of control group(P< 0.05); while HDL-Ch and Apo-A levels increased significantly after treatment which were obviously higher than those of control group(P< 0.05). Conclusion: The acupuncture method of strengthening the spleen to resolve phlegm can significantly raise clinical therapeutic effect in the treatment of wind-phlegm obstructing collateral type stroke, which may be related to its functions in regulating blood lipid metabolism.1026|0|3更新时间：2023-08-11
- 摘要：Objective: To observe the therapeutic effect of acupuncture for verruca plana. Methods: A total of 54 verruca plana patients were randomized into acupuncture group (n=31) and medication group (n=23). The main acupoints used were Ashi-point(s), Zusanli (ST 36), Xuehai (SP 10), Zhongzhu (TE 3), Quchi (LI 11) and Hegu (LI 4) that were punctured once every other day, with 10 times being a therapeutic course. In medication group, the patients were treated by oral administration of Moroxydine (0.2 g/time, t.i.d.) and external application of 0.5% Phthiobuzone cream (b.i.d.), with 30 days being a therapeutic course. Results: After two courses’ treatment, of the 31 and 23 cases in acupuncture and medication groups, 12 (38.71%) and 5 (21.74%) were cured, 13 (41.94%) and 7 (30.43%) were improved, and 6 (19.35%) and 11 (47.83%) failed, with the effective rates being 80.65% and 52.17% respectively. The therapeutic effect of acupuncture group was significantly superior to that of medication group (P<0.05). Conclusion: Acupuncture therapy works well in reducing the number and area of verruca plana, and its therapeutic effect is superior to that of medication.881|0|0更新时间：2023-08-11
- 摘要：The present paper reviews development of researches on the correlation between mast cells (MCs) and meridian-acupoints from 1) distribution and morphological characters of MCs, 2) effects of acupuncture on the degranulation, rejuvenation, number, and mediator release of MCs, 3) the relationship between the propagation of the stimulation-induced sensation along the meridian (PSISM) and MCs: a) cascade reactions of cytokines of MCs and their biological effects, b) release of mediators of MCs and PSISM. Meridian-collateral system and MCs have a close correlation in both the function and structure. MCs in the connective tissues are called as “the migration brain” and vary in functions. Whereas the regulative effect of acupuncture may involve the complicated system of the organism including multiple factors and multiple links. The involvement of MCs-related humoral factor in the regulative efficacy of acupuncture probably possesses remarkable species variation or individual difference, and variation in different functional states. The underlying mechanism of MCs involving acupuncture effect and PSISM phenomenon needs to be studied further.1418|0|15更新时间：2023-08-11
- 摘要：In the present paper, the authors review recent development of research on the acupuncture treatment of experimental spinal cord injury from 1) therapeutic methods and acupoint selection; 2) effect of electroacupuncture (EA) on the levels of some bioactive substances as endothelin, thromboxane A 2, nitric oxide, acetylcholine, nerve growth factor, neurotrophin-3, etc. in the micro-environment of the injured tissues; 3) effect of EA on the regeneration of nerves; 4) mechanism of EA in promoting the recovery of the injured spinal cord; and 5) prospect of acupuncture therapy for clinical application, and the existent problems of basic research. At the moment, EA therapy is often used as a remedy for spinal cord injury, and the most commonly used main acupoints are Jiaji (EX-B 2), and Dazhui (GV 14), Lingtai (GV 10), etc. of the Governor Vessel.784|0|9更新时间：2023-08-11
- 摘要：All of the immunoreactions including nonspecific inflammation, T-cell-mediated immunity, and humoral immunity play important roles in the onset and devolopment of wind-stroke. Through intervening some links of the immunologic process and reducing immunologic injury, acu-moxibustion has become one of the effective remedies for wind-stroke. This article reviews development of researches on the immunoregulation effect and mechanism of acu-moxibustion treatment in stroke patients and experimental cerebral hemorrhage animals from 1) immunoregulation of acu-moxibustion on human body, 2) effect of acu-moxibustion in relieving inflammatory reactions in acute cerebral hemorrhage animals, and 3) effect of acu-moxibustion on the specific immunity in acute cerebrovascular diseases.1056|0|4更新时间：2023-08-11
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