最新刊期
卷 ， 期 6 ， 2006
- 摘要：Objective: To observe the effects of acupoint-application and moxibustion pretreatment on serum inflammatory cytokine and hormones of rats with adjuvant arthritis at early and secondary stages.Methods: Forty Wistar rats were randomly divided into normal,early-stage model,secondary-stage model groups,early-stage pretreatment and secondary-stage pretreatment groups.Arthritis model was established by injecting Freund's complete adjuvant(FCA,(0.1 mL)) into the rat's right hind-paw.After removing the hair and before injecting FCA respectively,a plaster made of Herba Epimedii,etc. and an ignited moxa cone(placed on the plaster) were applied to "Dazhui"(GV 14),once every other day,8 times altogether.Rats of the 4 early-stage and secondary-stage groups were killed on the 3(rd) day and 16(rd) day and 16(th) day after injection of FCA respectively,and those of normal group also killed for collecting blood samples.The contents of serum IL-6,testosterone(T),growth hormone(GH) and cortisol(CORT) were assayed respectively with radioimmunoassay according to the instructions of reagent kits.Results: Compared with normal group,the contents of serum IL-6,GH and CORT of early-stage-model and secondary-stage-model groups increased significantly((P<0.01)),and those of early-stage pretreatment groups,and serum T and GH of secondary-stage pretreatment groupsalso increased remarkably((P<0.05,0.01));but T level of early-stage model group decreased significantly((P<0.05));while in comparison with the corre-sponding model groups,the contents of T in early-stage and secondary-stage pretreatment groups increased considerably((P<0.05,0.01));and those of serum IL6 and GH lowered markedly((P<0.05));no significant differences were found among the 2 model and 2 pretreatment groups((P>0.05)).Conclusion: Preconditioning of acupoint-application and moxibustion at GV 14 can effectively suppress arthritis induced increase of serum IL-6 and GH levels and decrease of T to modulate inflammatory cytokine and endocrine hormones, playing a protective role in adjuvant arthritis rats.1145|0|0更新时间：2023-08-11
- 摘要：Objective: To observe the effect of electroacupuncture(EA) on delta(δ)-opioid receptor gene expression in the inflammatory tissue in adjuvant arthritis rats so as to study the underlying peripheral mechanism of EA analgesia.Methods: Thirty-two SD rats were randomized into control,model,EA(model+EA) and normal+EA(normal animals) groups with 8 cases in each group.EA(2-(4 V),20-(100 Hz)) was applied to "Xuanzhong"(GB 39) and "Kunlun"(BL 60) for(20 min),once daily,and 6 sessions all together.Arthritis model was established by injection of Freund's complete adjuvant((0.1 mL)) in the rat's right hind-paw.The latency of radiation-heat irradiation induced leg withdrawal was considered as the pain threshold.At the end of experiments,the animals anesthetized with 3% phenobarbital((30 mg/kg)) were transcardiacally perfused with 4% paraformaldehyde and 0.1% diethylpyrocarbonate,followed by taking the local focus tissue.After routine treatment,the tissue samples were embedded with paraffin,cut into sections((6 μm)) and processed with in situ hybridization histochemistry for observing the expression of delta opioid receptor mRNA.Results: ① Compared with control group,the pain threshold of model group declined significantly((P<0.01)),while comparison between EA and model groups,and between normal+EA and model groups showed that the pain thresholds of two EA groups were significantly higher((P<0.01)),and the threshold of normal+EA group was also significantly higher than that of EA group((P<0.01)).No significant difference was found between control and EA groups in the pain threshold((P>0.05)),indicating that EA could raise the pain threshold in both arthritis and normal rats.② Compared with model group,the number of δ opioid receptor mRNA expression-positive cells of EA group was remarkably more((P<0.01)),showing marked upregulation of δ opioid receptor mRNA expression in the focal tissue after EA.Conclusion: EA has a definite analgesic effect in arthritis rats,which may be related to its effect in upregulating the expression of δ opioid receptor mRNA expression.950|0|0更新时间：2023-08-11
- 摘要：Objective: To explore the protective effect of electroacupuncture(EA) on the activity of cerebral cytochrome C oxidase and mitochondrial DNA(MtDNA) in cerebral ischemia-reperfusion(CI/R) rats.Methods: Forty-eight male SD rats were evenly randomized into shamoperation,sham-operation+EA,model,and model+EA groups with 12 cases in each group.CI/R model was established by middle cerebral artery occlusion(MCAO) under anesthesia(7% chloral hydrate,(1 mL)/(200 g)).EA(1-(4 mA),(5/20 Hz),dense-sparse waves) was applied to "Shuigou"(GV 26) and "Baihui"(GV 20) for(30 min) first,suspended for 15 min and then 30 min again.The rats in each group were sacrificed for taking out the right hemisphere of the brain and the cerebral mitochondria were isolated by differential centrifugation.Mitochondria suspension((1 mg/mL) protein) were frozen and thawed three times under temperature of(-20 ℃).The activity of complex IV(cytochrome C oxidase) was estimated by spectrophotometry and figured out according to formula: enzyme activity [μmol/(min·mg mitochondria protein)]= K·cytochrome C concentration/ protein concentration [K is the ratio between nature Logarithm of OD values and time(min) of six time points at the wavelength of 550 nm)].MtDNA was isolated and purified according to DNA isolation kit.The concentration of MtDNA was determined by spectrophotometry.DNA damage levels were measured by agarose gel electrophoresis.Results: Compared with sham-operation and sham-operation+EA groups,the activity of cytochrome C oxidase was significantly lower in model group((P<0.01)),while compared with model group,the activity of this enzyme in model+EA group was obviously higher(P<0.01), indicating an increase of the activity of cytochrome C oxidase after EA.There was no significant difference between sham and sham+EA in the activity of this oxidase.MtDNA in the core of the brain focus and penumbra region were both damaged seriously.DNA ladder was found in the core.DNA damage extent in the penumbra region reduced after EA treatment,but not in the core region.Conclusion: EA can reduce the injury of mitochondrial function,which may result from its effect in alleviating the oxidative stress of the brain cells in MCAO rats.1200|0|4更新时间：2023-08-11
- 摘要：Objective: To observe the effect of electroacupuncture(EA) on micturition frequency(MF),and the activity of pontine tyrosine hydroxylase(TH) and vasoactive intestinal peptide(VIP) in the sacral dorsal horn of the spinal cord in L-dopa induced bladder hyperactivity rats so as to investigate its neurobiological mechanism.Methods: A total of 44 female SD rats were randomized into normal(n=6),saline control(n=10),model(n=13) and EA(n=15) groups.Bladder hyperactivity model was established by intraperitoneal injection(i.p.i) of Carbidopa((5 mL/kg)) first and L-dopa((5 mL/kg)) 15 min later.Continuous cystometry was performed in conscious rats without any restraint for detecting MF.EA((5 Hz),4-(6 V),continuous waves) was applied to bilateral "Zhonglushu"(BL 29) for(30 min).At the end of the experiments,the rats were transcardiacally perfused with 4% paraformaldehyde under deep anesthesia(10% chloral hydrate),followed by sampling the pons and the sacral cord tissues and cutting into sections ((50 μm and 30 μm respectively)).The activity of TH in pontine tissue and VIP in the sacral dorsal horn of the spinal cord was assayed with immunohistochemical method(ABC method).Results: In comparison with control group,ratios of MF in model group increased significantly(15-45 min),(46-75 min) and(76-105 min) after injection of L-dopa(P<0.05),while compared with model group,MF values of EA group declined considerably(4675 min) and(76-105 min) after injection of L-dopa(P<0.05),suggesting an improvement of L-dopa-induced bladder hyperactivity by EA.Compared with normal group and control group,the relative area values of VIP immunoreaction(IR)-positive fibers and terminals in the sacral cord and TH in pontine tissue increased slightly((3 h)) and significantly((8 h),(P<0.05)) after i.p i.of L-dopa;while compared with model group,the value of VIP increased notably(3 h) after i.p.i.and those of VIP(8 h) after i.p.i.of L-dopa, and TH(3 h) and(8 h) after i.p.i.of L-dopa decreased markedly((P<0.05)),suggesting that EA could promote the release of VIP from the somatic afferent and inhibit the synthesis of TH in pontine micturition center.Conclusion: EA can inhibit L-dopa induced bladder hyperactivity,raise the VIP activity of sacral cord(3 h) after i.p.i.of L-dopa,and reduce VIP activity(8 h) after i.p.i.and TH activity(3 h) and(8 h) after i.p.i.,which maybe the underlying mechanism of EA in inhibiting bladder hyperactivity by reducing the release of TH from pontine micturition center and promoting the release of VIP from the sacral cord dorsal horn.1148|0|0更新时间：2023-08-11
- 摘要：Objective: To observe the effect of acupuncture on tear secretion and the concentration of vasoactive intestinal polypeptide(VIP) in the lacrimal gland tissue in the rabbit.Methods: A total of 16 female New Zealand rabbits were randomized into control and acupuncture groups with 8 cases in each group."Taiyang"(EX-HN 5),"Cuanzhu"(BL 2) and "Sizhukong"(TE 23) on the right side were punctured with filiform needles and stimulated for a while with hand by lifting,thrusting and twirling the needle repeatedly.The needles were retained for 20 min and the treatment was conducted 3 times a week with 10 sessions being a therapeutic course.The Schirmer 1 test(S1T,reflecting the basic secretion function) was used to check the secretion function of tear before,immediately and 3 days after acupuncture treatment.The lacrimal gland tissue was sampled and homogenated for assaying the concentrations of VIP before and after acupuncture treatment with radioimmunoassay.Results: Compared with pre-acupuncture the S1T value of acupuncture group increased notably immediately and 3 days after acupuncture [((17.89±3.06) mm/5 min),((16.89±4.46) mm/5 min) vs((10.44±1.81) mm/5 min),(P<0.01)],suggesting an obvious increase in the secretion of tear after acupuncture stimulation.Radioimmunoassay showed that compared with control group the average concentration of VIP in normal lacrimal gland tissue in acupuncture group was significantly higher [((109.75±22.32) pg)/(100 μg) vs((62.71±20.12) pg/100 μg),(P<0.001)].Conclusion: Acupuncture can increase the secretion of tear and raise the concentration of VIP in lacrimal gland tissue,suggesting a direct interaction between VIP and tear secretion from the lacrimal gland.Besides,the influence of acupuncture on the secretion of tear has a long-term aftereffect.890|0|3更新时间：2023-08-11
- 摘要：Objective: To research the effect of preventative moxibustion(PM) of "Guanyuan"(CV 4) on nitric oxide synthase(NOS) activity in the plasma and uterus tissue in menopausal rats.Methods: A total of 70 female SD rats(aged 10 months) were randomized into 10-months(mo) control(con),12-mo-con,14-mo-con and 16-mo-con,12-moPM,14-mo-PM and 16-mo-PM groups with 10 rats in each group.In addition,other 10 female SD rats aged 4 mo were used as young-con group.In rats of 3 PM groups,moxibustion(one moxa-cone/time) was applied to "Guanyuan"(CV 4) twice a week,8 weeks altogether.At the end of experiments all the rats were decapitated for collecting blood samples and uterus tissues.NOS activities in plasma and the homogenized uterus tissue supernate were detected with nitrate reductase method.Results: In comparison with young-con group,NOS activities of both plasma and uterus tissue in 14-mo-con group,and those of uterus in 16-mo-con group,14-mo-PM and 16-mo-PM groups decreased significantly((P<0.05,0.01)). Comparison between 14-mo-con and 14-mo-PM groups and between 16-mo-con and 16-mo-PM groups indicated that plasma NOS activity of 14-mo-PM group and uterus NOS activity of 16-mo-PM group were significantly higher than those of the former groups,suggesting that preventive moxibustion could inhibit the decline of NOS levels in blood and uterus tissue.Conclusion: Preventive moxibustion of CV 4 can effectively relieve the decline of plasma and/or uterus NOS levels in menopausal rats.1157|0|5更新时间：2023-08-11
- 摘要：Objective: To observe the therapeutic effects of acupuncture for depressive neurosis by using liver-function-regulating method.Methods: A total of 201 patients were randomized into acupuncture group(n=78),medication(Prozac) group(n=82) and sham-acupuncture group(n=41).Acupoints used were Taichong(LR 3),Hegu(LI 4),Baihui(GV 20),Yintang(EX-HN 3),otopoint Liver and Heart.For sham-acupuncture group,the punctured spots were those(0.5 cm) to LR 3 and(LI 4) on the radial side,(0.5 cm) to(GV 20) on the left side,(0.5 cm) to EX-HN 3 on the left side.Acupuncture treatment was given twice every week,12 weeks altogether.Patients of medication group were ordered to take Prozac(20 mg/d),continuously for 12 weeks.Hamilton Rating Scale for Depression(HAMD) and Self-rating Depression Scale(SDS) were used to evaluate the therapeutic effect.Results: After the treatment,of the 78,82 and 41 cases in acupuncture, medication and sham-acupuncture groups,7(9.0%),10(12.2%) and 1(2.4%) were cured,55(70.5%),33(40.2%) and 13(31.7%) experienced marked improvement in their symptoms,14(18.0%),25(30.5%) and 12(29.3%) had improvement,2(2.5%),14(17.1%) and 15(36.6%) failed,with the effective rates being 97.5%,82.9% and 63.4% respectively.The effective rate of acupuncture group was significantly higher than those of medication group and sham-acupuncture group ((P<0.01)),and that of medication group was markedly higher than that of sham-acupuncture group((P<0.05)).Scores of HAMD and SDS of the 3 groups all decreasedapparently after the treatment,and those of acupuncture group were significantly lower than those of sham-acupuncture and medication groups((P<0.05)),showing that the effect of acupuncture was significantly superior to that of Prozac in improving depression patients' clinical symptoms.HAMD and SDS scores of medication group were significantly lower than those of sham-acupuncture group((P<0.05)).Conclusion: Acupuncture therapy is effective in improving symptoms of depression patients and lowering both HAMD and SDS scores.1091|0|13更新时间：2023-08-11
- 摘要：Objective: To observe the therapeutic effect of combined acupuncture therapy on dysphagia due to pseudobulbar palsy in stroke patients.Methods: A total of 95 stroke patients were randomly divided into combined acupuncture therapy group(n=54) and medication group(n=41).Patients of the combined acupuncture therapy group were treated with scalp-acupuncture,body acupuncture,acupoint-injection(Cobamanlide,(0.5 mg/mL)) and medicines as Red Sage Root Injection((800 mg),i.v.,once daily),Aspirin((300 mg) for the first 15 days,and(100 mg) thereafter,PO,once daily),Nimotop((30 mg),PO,3 times per day),etc.Scalp-points used were bilateral Motor Area(MS 1),body acupoints were Baihui(GV 20),Lianquan(CV 23),Tongli(HT 5),Fenglong(ST 40),Taichong(LR 3) and Zusanli(ST 36).Electroacupuncture(EA,3-(5 mA),10-(50 Hz)) was applied to MS 1,HT 5 and(ST 40) for 30 min.Cobamanlide((0.5 mL)/point) was injected into Zusanli(ST 36).The treatment was given once daily with 15 times being a therapeutic course and 2 courses altogether.Patients of medication group were treated with simple medicines.The therapeutic effect was evaluated according to Professor SHI's criteria. Results: After one month of treatment,of the 54 cases and 41 cases in treatment and medication groups,24(44.4%) and 11(26.8%) were cured,21(38.9%) and 13(31.7%) had marked improvement,4(7.4%) and 5(12.2%) had improvement,5(9.3%) and 12(29.3%) failed respectively.The effective rate, total scores,and scores of swallowing-drinking function,masticatory function in combined acupuncture therapy group were significantly higher than those in medication group((P<0.05),90.7%,16.35±3.96,2.67±1.10,(2.61±)(1.19 vs) 70.7%,13.83±5.58,2.12±1.25 and 2.07±1.21). Conclusion: The therapeutic effect of combined acupuncture treatment is significantly superior to that of simple medication in improving symptoms and signs as dysphagia due to pseudobulbar palsy,etc.in patients with stroke.921|0|5更新时间：2023-08-11
- 摘要：Objective: To observe the therapeutic effect of acupuncture in the treatment of acute gouty arthritis so as to seek an effective therapy for it.Methods: A total of 130 patients suffering from acute gouty arthritis were randomized into acupuncture group(66 cases) and medication group(64 cases).In acupuncture group,bilateral Bafeng(EX-HE 10),Sanyinjiao(SP 6),Yinlingquan(SP 9),Xuehai(SP 10),etc.were punctured by inserting the needle rapidly,twirling it with reducing technique and removing it swiftly(needling for clearing heat and eliminating dampness and stasis).The treatment was given twice daily in the first 10 days and once daily in the second 10 days.Patients of medication group were ordered to orally take Meloxicam Capsules((7.5 mg)/time/d,Non-steroid antiphologistic) continuously for 20 days.Visual analog scale(VAS) and changes of clinic manifestations were recorded,and blood uric acid,urine uric acid and uric β_2-microglobulin were detected with enzymatic method,phosphotungstic acid reduction method and solid phase radioimmunoassay respectively.Results: Of the 66 and 64 cases in acupuncture and medication groups,37(56.06%) and 14(21.88%) were cured,26(39.39%) and 45(70.31%) had marked improvement in clinical symptoms,3(4.55%) and 5(7.81%) failed in the treatment separately.The cure rate of acupuncture group was significantly higher than that of medication group ((P<0.01)).After the treatment VAS,blood uric acid and uric β_2-microglobulin values of two groups decreased nearly all obviously((P<0.05)),while urine uric acid increased significantly in acupuncture group((P<0.01));and the effects of acupuncture were significantly better than those of medication in lowering blood uric acid and uric β_2-microglobulin,and raising urine uric acid((P<0.01)).No significant differences were found between two groups in VAS((P>0.05)).Conclusion: Acupuncture treatment is one of the best therapies in dealing with acute gouty arthritis and can help improve renal impairment in the early stage of this disease.927|0|2更新时间：2023-08-11
- 摘要：In the present paper,the authors,in the light of Yin-Yang theory of Chinese medicine,analyze the pathogenesis of apoplectic spastic-paralysis from a) imbalance between yin and yang,b) impairment of yang-qi,c) malnutrition of muscles and tendons,and d) deficiency in origin and excess in superficiality.In view of the abovementioned analysis,the principles for selecting acupoints are 1) yin-yang balancing method by selecting acupoints on the healthy side of the body as the main acupoints in combination with those on the affected side,and taking acupoints of the yin meridians as the main acupoints in combination with those of yang meridians,which includes a) scalppoints for regulating yin and yang,b) opposing needling for regulating yin and yang,c) paired needling for modulating yin and yang,d) penetrative needling for modulating yin and yang,and e) motor-tension-regulating needling for balancing yin and yang;2) musculotendon needling,3) yang-warming and-reinforcing needling.In the coming days,more clinical studies on acupuncture treatment of apoplexy by using international standards should be conducted further so as to raise the therapeutic effect and improve the patient's life quality.1914|0|3更新时间：2023-08-11
- 摘要：In the present paper,the authors sum up the progress of researches on 3-dimensional(D) reconstruction of acupoints in recent years from 1) 3D reconstruction according to the laminated sections of the local tissue after labeling the single acupoint;2) 3D reconstruction of acupoints according to the data collected from the current virtual human body;and 3) 3D reconstruction of acupoints by taking the virtual human as its operational platform.In view of the technique,we consider that for 3D reconstruction of acupoints,the virtual human being as an operational platform is suitable because the operator can devote themselves to studying the anatomic structure and also can present all kinds of tissues perfectly and systematically in the 3D construction of acupoints by applying the knowledge of computer graphics and establishing the mathematic model for nerves and blood vessels that are difficult to reconstruct currently.754|0|2更新时间：2023-08-11
- 摘要：In the present paper,the authors reviewed progress of researches on the influence of stimulating parameters of electroacupuncture on acupuncture analgesia from wave forms,frequency,intensity,duration of stimulation.Electroacupuncture anesthesia has advantages of easier operation,lighter work load,higher safety and better outcomes.The analgesic effect of electroacupuncture is the foundation of acupuncture anesthesia,and standardized,generally acknowledged and more effective stimulating parameters should be researched further.841|0|16更新时间：2023-08-11
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