最新刊期
卷 ， 期 3 ， 2007
- 摘要：Objective: To investigate the role of mast cells in acupuncture analgesia in rats.Methods: A total of 48 Sprague Dawley(SD) rats were divided into normal control,acupoint [("Zusanli"(ST 36)]-acupuncture(Acu),non-acupoint(3 mm left to ST36),disodium cromoglycate(DSC,0.02 g/mL,for acupoint injection),normal saline(NS,for acupoint injection),DSC+Acu,NS+Acu and DSC+contralateral Acu groups,with 6 cases in each group.The latency of tail flick response to heat irradiation was used as the pain threshold."Zusanli"(ST 36) was punctured with filiform needle and stimulated by lifting and thrusting the needle for 30 min.After sacrifice under anesthesia(1% embutal),tissues of ST36 area were sampled,sliced(4 μm),and stained with Toluidine Blue for skin and Neutral Red for muscles.Results: Compared with normal control group,the ratios of pain threshold increased significantly in all the 7 experimental groups(P<0.05),and those of DSC and NS groups were significantly lower than those of acupoint-Acu,non-acupoint,DSC+Acu,NS+Acu and DSC +contralateral Acu groups(P<0.05).Compared with DSC+Acu group,the ratios of NS+Acu and DSC+contralateral Acu groups were evidently higher(P<0.05,0.01).Compared with control group,the degranulation ratio of acupoint-Acu group was significantly higher(P<0.05,0.001 in muscle and skin separately),and the ratio of DSC+Acu group was markedly lower than that of acupoint-Acu group(P<0.01 in skin).Conclusion: Acupuncture of ST36 has a significant analgesia and enhances the degranulation of mast apparently cells,which is weakened by injection of DSC in the acupoint area,suggesting an important role of mast cells in acupuncture-induced analgesia.关键词：Acupoint;Acupuncture analgesia;Mast cell degranulation;Disodium cromoglycate1268|0|23更新时间：2023-08-11
- 摘要：Objective: To observe the effect of acupuncture in combination with Paclitaxel on apoptosis in Lewis lung carcinoma mice so as to investigate their anti-cancer mechanism.Methods: A total of 48 mice with inoculated Lewis lung carcinoma(LLC) were randomized into model,acupuncture,medication and acupuncture +medication groups,with 12 cases in each group.LLC model was established by subcutaneously incubating LLC cells into the left hindlimb of the C57BL/6 mice."Feishu"(BL13) was punctured and stimulated manually by manipulating the acupuncture needle with small amplitude for 1 min,twice again,followed by intraperitoneal injection of Paclitaxel(10 mg/kg).The treatment was given once daily,continuously for 10 days.The tumor weight was recorded,the inhibitory rate of LLC calculated and the apoptosis of LLC cells displayed with TUNEL method.Results: Compared with model group,the tumor weight values of acupuncture,medication and acupuncture +medication groups were significantly lower(P<0.05),and the tumor inhibitory rates and the apoptotic index(AI) were markedly higher(P<0.05).Comparison among acupuncture,medication and acupuncture +medication groups showed that the tumor weight values of the later 2 groups were obviously lower than that of acupuncture group,and that of acupuncture +medication group was apparently lower than the weight of medication group(P<0.05); while the inhibitory rate of tumor and AI in acupuncture +medication group were significantly higher than those in acupuncture and medication groups(P<0.05).The findings showed that acupuncture,medication and acupuncture +medication could significantly suppress the growth of tumor and promote apoptosis of LLC cells,and the effect of the combined treatment group was significantly superior to that of simple acupuncture and medication.Conclusion: Combination of acupuncture and Paclitaxel has a synergistic effect in inhibiting the growth of LLC and accelerating apoptosis of tumor cells.关键词：Lewis lung carcinoma;Acupuncture;Paclitaxel;Anti-carcinoma999|0|2更新时间：2023-08-11
- 摘要：Objective: To study if the spinal glial cells involve in the protective effect of acupuncture on gastric mucosa in high humid heat stress rats.Methods: Thirty-six male SD rats were randomized into 6 groups: control,stress model,electroacupuncture(EA),Fluorocitrate(FCA,intrathecal injection of FCA,1 μL,60 min before humid heat stress),EA+normal saline(NS,intrathecal injection of NS) and EA+FCA groups.Stress model was established by putting the rats in a container with higher temperature and higher humidity [(40.0±0.5) ℃,relative humidity(60±5) %] for 60 min.EA(50 Hz,intermittent waves,2-5 V) was applied to bilateral "Zusanli"(ST 36) for 60 min.Using immunofluorescent methods,we observed glial fibrillary acidic protein(GFAP) and microglia OX42 immunoreactivity(OD value) in the tissue of the lumbar enlargement segment of the spinal cord.We evaluated and recorded the damage index(DI) of gastric mucosa of rats according to Guth's method.Results: There were clear dot-line-like hemorrhage foci with formation of ulcer in the gastric mucosa 60 minutes after high humid heat stimulation. Compared with model group,DI of gastric mucosa in EA and EA+NS groups decreased significantly(P<0.05,suggesting a protective effect of EA on the gastric mucosa under stress),OD values of EA and EA+NS groups increased considerably(P<0.05).Comparison between EA and EA+FCA groups showed that DI of EA+FCA group was higher than that of EA group, while the OD values of GFAP and OX42 in EA+FCA group was markedly lower than those in EA group(P<0.05),suggesting an inhibitory effect of FCA on the effects of EA in improving stress-induced damage of gastric mucosa and upregulation of GFAP and OX42 expression.Conclusion: EA at "Zusanli"(ST 36) can prevent the gastric mucosa from injury caused by high humid heat stimulation.The lumbar spinal glial cells may play a role in EA's protective function.关键词：Stress;Gastric mucosa;Electroacupuncture;GFAP & OX42 expression;Spinal glial cells1039|0|1更新时间：2023-08-11
- 关键词：Cerebral ischemia-reperfusion;Cerebral cortex neurons;Mitochondria;Xiusanzhen;Electroacupuncture1063|0|5更新时间：2023-08-11
- 摘要：Objective: To investigate the effect of electric mildly-warmed needle of inner Mongolian medicine on changes of free radical metabolism in the liver tissue of rats with fatigue.Methods: A total of 33 male SD rats were evenly randomized into control,model and electro-mildly-warmed needle(EMWN) groups.Fatigue model was established by forcing the rat to swim in a water pool till exhaustion,once daily,continuously for 21 days."Dinghui"(central spot of the bregmatic bone) and "Xinxue"(the center of the depression beneath the 7th thoracic vertebra) were punctured with silver needle which was warmed electrically by using a MLY-I Electrical Needle-warming Apparatus,once every 3 days,7 sessions altogether.After decapitation,the rat's liver was taken,homogenated and centrifuged for detecting malondialdehyde(MDA) content with thio-barbituric acid,reduced glutathione(GSH) contents,glutathione peroxidase(GSH-Px) activity with chromatometry and superoxide dismutase(SOD) activity with xanthine oxidase method respectively.Results: Compared with control group,liver GSH content,GSH-PX and SOD activity in model group lowered significantly(P<0.05,0.01),while in comparison with model group,these 3 indexes of EMWN group increased considerably(P<0.05,0.01).No significant differences were found among 3 groups in MDA levels(P>0.05).The results showed that the effect of mildly-warmed needle treatment could resist fatigue induced decline of activity of antioxidant enzymes.Conclusion: Electric mildly-warmed needle therapy of inner Mongolian medicine can raise the activity of antioxidant enzymes in liver tissue of fatigue rats,which may underlie the action of mildly-warmed needle in improving fatigue.关键词：Fatigue;Electric mildly-warmed needle;Liver free radicals922|0|2更新时间：2023-08-11
- 摘要：Objective: To observe the effect of warmth-promotion needling on cerebral malondialdehyde(MDA) content,superoxide dismutase(SOD) and acetylcholinesterase(AChE) activity so as to explore its underlying mechanism in improving vascular dementia(VD).Methods: Forty Wistar rats were randomized into control,model,medication,needle-twirling and warmth-promotion groups with 8 cases in each.VD model was established by occlusion of the bilateral common carotid arteries and reperfusion in combination with intraperitoneal injection of sodium nitroprusside(2.5 mg/kg).Warmth-promotion needling was applied to "Dazhui"(GV 14),"Baihui"(GV 20) and "Shuigou"(GV 26).Rats of medication group were intragastric perfusion of Nimodipine suspension(0.0108 g/kg).The treatment was given once daily continuously for 15 days.SOD and AChE activity and MDA content of the brain(right side) tissue were detected with purine oxidase method,hydroxylamine chromatometry and thio-malonylurea method separately.Results: In comparison with control group,SOD activity of model group decreased significantly(P<0.01),AChE activity and MDA content increased considerably (P<0.01);while compared with model group,SOD activity of warmth-promotion,needle-twirling and medication groups raised evidently(P<0.01),AChE activity and MDA content of the later 3 groups lowered significantly(P<0.05,0.01).The effects of warmth-promotion group were significantly superior to that of medication group in lowering MDA content and to those of needle-twirling group in raising SOD activity and lowering AChE activity and MDA content(P<0.05).Conclusion: Warmth-promotion needling can function well in resisting lipid peroxidation injury and lowering AChE activity in VD rats,which may contribute to its effect in improving learning-memory ability.1033|0|4更新时间：2023-08-11
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Effect of Acupoint Application Therapy on Expression of IL-4 and IFN-γ in Lung Tissue of Asthma Rats
摘要：Objective: To observe the effect of acupoint application of Chinese herbal medicines on pathological changes of pulmonary tissue in asthma rats so as to explore its underlying mechanism.Methods: A total of 33 Wistar rats were randomized into normal control(n=6),model(n=9),dexamethasone(DX,n=9) and acupoint-application(A-A,n=9) groups.Asthma model was established by intraperitoneal injection of 10% ovalbumin(OVA,1 mL)and forced inhalation of atomized 2% OVA(25 mL) for 40 min,once daily for 2 weeks."Dazhui"(GV 14),"Pishu"(BL 20),"Feishu"(BL 13) and "Shenshu"(BL 23) were selected for external application of Chinese medicinal herbs(pricklyash peel,white mustard seeds,asarum herb,etc.) for rats of A-A group,and that of control drugs(ang-kak,black rice,ginger juice,etc) for rats of DX group.Infiltration degrees of eosinophils(Eos),lymphocytes(L) and macrophages(M) in the pulmonary tissue were observed under microscope.IL-4 and IFN-γ expression was displayed by using immunohistochemical method.Results: In comparison with control group,the infiltration degrees of Eos,L and M increased significantly in model,DX and acupoint-application groups(P<0.05,0.01);while compared with model group,the infiltration of Eos,L and M in DX group,Eos in A-A group decreased significantly(P<0.05,0.01).No significant differences were found between DX and A-A groups in these 3 indexes(P>0.05).Results of immunohisto chemical staining in the lung tissue indicated that compared with control group, IL-4 expression in model increased significantly(P<0.01),IFN-γ expression decreased considerably in model and DX group(P<0.01);when compared with model group,IL4 in both A-A group and DX group decreased significantly,and IFN-γ expression in A-A group increased considerably(P<0.05,0.01).No significant differences were found between DX and A-A groups in IL-4 expression(P>0.05).Conclusion:Acupoint application of Chinese herbal medicines can balance Th1/Th2 and effectively reduce the infiltration of eosinophils,which may contribute to its therapeutic effect in relieving asthma.1078|0|4更新时间：2023-08-11 - 摘要：Objective: To compare the therapeutic effects of acupuncture of "Wuyi"(ST 15) and multi-acupoints for hyperplasia of mammary glands so as to choose the key acupoints for cyclomastopathy.Methods: A total of 48 female Wistar rats were randomized into normal control,model,"Wuyi"(ST 15) and multi-point groups with 12 cases in each group.Cyclomastopathy model was established by intraperitoneal injection of benzestrofol(0.5 mg/kg per day) for 25 days,followed by administration of flavolutan(4 mg/kg per day,i.p.) for 5 days.Bilateral ST15 were punctured and stimulated manually for ST15 group;while for multi-point group,bilateral ST15,"Hegu"(LI 4),"Zusanli"(ST 36) and "Shanzhong"(CV 17) were punctured,once daily,27 sessions altogether.After decapitation,the rats' second pairs of mammary glands were removed to be fixed routinely,embedded in paraffin,sectioned(5 μm),and stained with H&E method for observing the structural changes under microscope and with norphometry.Results: Compared with control group,the diameter and area of acinar lumina(AL) of mammary glands in model group increased significantly(P<0.01),whereas those in both ST15 and multi-point groups were significantly lower than those 2 indexes in model group(P<0.01).No significant differences were found between ST15 and multi-point groups in the diameter and area of AL.Conclusion: "Wuyi"(ST 15) can effectively suppress estrogenic hormones-induced hyperplasia of mammary glands and is thus a key acupoint for treatment of cyclomastopathy.关键词：Cyclomastopathy;Acupuncture;Wuyi(ST 15);Norphometry842|0|1更新时间：2023-08-11
- 摘要：Objective: To confirm the better analgesic effect of otopoint-penetrative needling for cervical type and nerve-root type cervicospondylopathy.Methods: A total of 98 cervicospondylopathy outpatients(50 cases of cervical type and 48 cases of nerve-root type) were randomly divided into treatment group(otopoint-penetrative needling) and control group(otopoint-straight needling) in the light of paring method of comprehensive factors of sexes,ages and the state of disease.The main otopoints used were bilateral Jingzhui Area(AH 13) in combination with Jian-Jianguanjie-Suogu(Shoulder-Shoulder-joint-Collarbone) Area,etc.The simplified McGill Pain Scaling was used to give the score of patient's pain before the treatment,5 min and 30 min after the treatment.Results: Results of sequential trial indicated that the analgesic effect of otopoint-penetrative needling was significantly superior to that of otopoint-straight needling 30 min after the treatment (P<0.05).Findings of matched-pair t test showed that no marked differences were found between two groups in the pain scores before the treatment,while after the treatment,the pain scores of otopoint-penetrative needling group were significantly lower than those of otopoint-straight needling group(P<0.001,0.01),meaning that the analgesic effect of otopoint-penetrative needling was significantly better than that of otopoint-straight needling 5 min and 30 min after the treatment in both men and women,in both cervical type and nerve-root type patients,and in both young and older patients.Conclusion: The analgesic effect of otopoint-penetrative needling is obviously superior to that of otopoint-straight needling.关键词：Cervicospondylopathy;Otopoint-penetrative needling;Otopoint-straight needling;Auricular acupuncture1257|0|10更新时间：2023-08-11
- 摘要：Objective: To observe the therapeutic effect of cluster-needle stimulation of scalp-points combined with rehabilitation training for apoplectic aphemia.Methods: A total of 56 outpatients were randomized into control(medication,manicol/ beronald,Ca2+ antagonist,citicoline,etc.) group,rehabilitation(Rehab,visual-listening,articulation and speech training) group and acupuncture [Dingqu: 1 cun and 2 cun parallel to the line joining Baihui(GV 20) and Qianding(GV 21) respectively on the bilateral sides,Dingqianqu: 1 cun and 2 cun parallel to the line joining GV21 and Xinghui(GV 22) separately on both sides,etc] combined with rehabilitation(Acup+Rehab) group.Aphasia Battery of Chinese(ABC) was used to assess the patient's speech ability,i.e.,aphasia quocient(AQ);The Chinese functional communication(CFCP) test was used to evaluate the patient's daily life speech communication ability,and Boston Diagnostic Aphasia Examination(BDAE) was also conducted to assess the severity of aphasia.Results: After the treatment,the speech ability,oral presentation,listening comprehension,writing ability,AQ index and CFCP were all improved significantly in 3 groups(P<0.05,0.01);the total effective rate(85.00%) of Acup+Rehab group was significantly higher than those of Rehab group(77.78%) and control group(64.71%,P<0.05,0.01).The scores of ABC,AQ index,CFCP and BDAE grade in Acup+Rehab group were significantly higher than those of Rehab and control groups(P<0.05,0.01),and those scores of Rehab group were also significantly higher than those of control group(P<0.05,0.01).Conclusion: The cluster-needle puncture of scalp-points combined with rehabilitation training is an effective therapy for improving apoplectic aphemia.关键词：Apoplectic aphemia;Cluster-needle puncture;Scalp-acupuncture;Rehabilitation1011|0|7更新时间：2023-08-11
- 摘要：Objective:To observe the therapeutic effect of acupuncture therapy for treatment of cervical hypertension.Methods: Sixty cases of cervical hypertension patients were randomly divided into treatment group and control group by using random table method.For patients of treatment group bilateral cervical Jiaji(EX-B 2) were punctured,and for those of control group,Fengchi(GB 20),Taichong(LR 3),Quchi(LI 11),Zusanli(ST 36) and Sanyinjiao(SP 9) were punctured.Changes of blood pressure and clinical symptoms were recorded before and after the treatment.Results: Of the two 30 cases in treatment and control groups,18(60.0%) and 13(43.3%) had a remarkable hypotensive effect,10(33.3%) and 9(30.0%) had a hypotensive effect,2(6.7%) and 8(26.7%) had no apparent changes in blood pressure;12(40.0%) and 5(16.7%) experienced marked improvement in clinical symptoms,15(50.0%) and 16(53.3%) were effective,3(10.0%) and 9(30.0%) failed in the treatment,with the effective rates being 90.0% and 70.0% respectively.The therapeutic effect of treatment group was apparently superior to that of control group in lowering blood pressure and the cumulative scores of clinical symptoms and in improving clinical symptoms(P<0.05,0.01).Conclusion: Acupuncture of Jiaji(EX-B 2) is an effective therapy in the treatment of cervical hypertension.关键词：Acupuncture;Cervical hypertension;Cervical Jiaji(EX-B 2)1007|0|5更新时间：2023-08-11
- 摘要：Objective: To investigate the therapeutic effect and mechanism of electroacupuncture(EA) in treating gastrointestinal disorder in acute pancreatitis(AP) patients.Methods: A total of 94 cases of AP patients were divided into acupuncture group(n=56) and control group(n=38).The severity of AP was evaluated according to APACHE Ⅱ and Balthazar CT scoring system.EA(4 Hz,4-6 V) was applied to bilateral Zusanli(ST 36),Shangjuxu(ST 37),Xuanzhong(GB 39),Taichong(LR 3),and Gongsun(SP 4) for 60 min,twice a day,5 days altogether.Total and segmental colonic transit time(CTT) were determined by using ingestion of radiopaque markers(SITZMARKS) according to the modified Metcalf's method,serum motilin(MTL),cholecystokinin(CCK),vasoactive intestinal peptide(VIP) contents were assayed using enzyme linked immunosorbent assay(ELISA).Results: Compared with normal values,total and segmental CTT of AP patients(control group) increased apparently(P<0.05),especially in right colon,serum MTL and CCK contents in both control and treatment groups on the 1st day decreased considerably(P<0.05),while serum VIP levels of both control and treatment groups on the 1st day increased markedly(P<0.05).In comparison with control group,total and segmental CTT of treatment group decreased significantly(P<0.05).Auto-comparison of both control and treatment groups showed that serum MTL and CCK contents on day 9 were significantly higher than those on day 1(P<0.05),while serum VIP contents on day 9 in these two groups were both obviously lower than those on day 1(P<0.05).No significant differences were found between treatment group and control group in serum MTL,CCK and VIP levels on the 9th day after the treatment(P>0.05).Conclusion: Acupuncture is able to enhance the gastrointestinal dynamics,improve its motor activity.关键词：Electroacupuncture;Acute pancreatitis;Gastrointestinal dynamics;Gastrointestinal hormones988|0|11更新时间：2023-08-11
- 摘要：Objective:To observe the effect of electroacupuncture(EA) on serum testosterone(T),estradiol(E2),luteotropic hormone(LH),follicle-stimulating hormone(FSH),progesterone(P),and prolactin(PRL) in patients with Sjgren's Syndrome(SS) in order to analyze the correlation between the adjustment effect of EA and changes of hormone levels.Methods: Fifty-seven middle-aged and old women with SS were divided into medication group(n=27) and acupuncture group(n=30).Patients in acupuncture group were treated with EA(80 Hz,2.5 mA) of Shenshu(BL 23),Taixi(KI 3),Hegu(LI 4),etc.for 30 min,once every other day,and those of medication group were treated with oral administration of hydroxyl-chloroquine(6 mg/kg per day),oral transfer agent liquid,Vit B1,Vit B2,Vit C,fish-liver oil, one tablet/time,t.i.d.,and pilocarpine(20 mg/d),continuously for 2 months.Venous blood samples were collected before and after the treatment to examine contents of the abovementioned sex hormones by using electro-chemiluminescent immunoassay(CLIA). Results: 1) Before the treatment,no significant differences were found between two groups in the levels of serum T,E2,LH,FSH,P and PRL(P>0.05).After the treatment,self-comparison of each group showed that serum E2 and T contents in acupuncture group increased significantly(P<0.01) and serum LH content decreased significantly(P<0.01);while in medication group,only serum LH decreased markedly(P<0.05) in comparison with its basic value of pretreatment.No significant changes were found in serum P,FSH and PRL levels in both groups after the treatment(P>0.05).The results suggested that the therapeutic effect of acupuncture group was better than that of medication group in regulating multiple sex hormones.Conclusion: EA can effectively adjust E2,T and LH levels in Sjgren's Syndrome patients and improve most patients' clinical symptoms,and the therapeutic effect of EA is better than that of medication.关键词：Sjgren's Syndrome;Sex hormones;Electroacupuncture868|0|1更新时间：2023-08-11
- 关键词：Master DOU's Canon of Acupuncture;Published time;Academic source745|0|3更新时间：2023-08-11
- 摘要：The meridian-collateral system of traditional Chinese medicine refers to the regulating system composed of the known loose connective tissue,liquid-Qi of tissue,energy substances,nerves,blood vessels,lymph,etc.existing in the fascia space between the skin,muscles and bone and some associated unknown synthetic functions.This hypothesis of meridian-collateral,Qi-passage,and fascia space composed of multiple substances is probably able to unify different viewpoints about that the meridian-collateral system is in fact an integrated substance of nerves,body fluid and energy.In the present paper,the authors introduce their viewpoints about the essence of meridian-collaterals from nerves,humor and energy,and unknown function of the known anatomical structure.关键词：Meridian Qi-passage;Intra-meridian Qi;Meridian essence1305|0|5更新时间：2023-08-11
- 摘要：The present paper reviews development of researches on the relationship between mast cells(MCs) and effects of acupuncture and moxibustion from 1) the formation,distribution,types,morphological characters of MCs and their biological effects,2) the number and degranulation of MCs in acupoints,3) the relavance of MCs and the phenomenon of sensation transmission along meridian,and 4) the correlation between the therapeutic effect of acupuncture for relieving allergic reaction and MCs.Researches show that 1) MCs act as one of the characteristic constituents in acupoints where the MCs are in close relation with the effects of acupuncture and moxibustion,2) MCs participate in the regulatory process of the organism in physiology and pathology.The underlying mechanism of MCs-related neuro-humoral regulation and meridian needs to be studied further.关键词：Mast cells;Acupuncture and moxibustion;Acupoints;Degranulation1095|0|6更新时间：2023-08-11
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