最新刊期
卷 ， 期 1 ， 2008
- 摘要：Objective To explore the possible central and peripheral mechanisms of connexin 43 gene knockout in acupuncture analgesia.Methods Fifty-four wide type(WT) mice and 54 connexin 43 gene knockout(heterozygote,HT) mice were randomly divided into WT control group,WT model group,WT acupuncture group,HT control group,HT model group and HT acupuncture group(n=18/group).Visceral pain model was established by intraperitoneal injection of 0.6% acetic acid(0.1 mL/10 g)."Zhongwan"(CV 12) and bilateral "Zusanli"(ST 36) were punctured with filiform needles and stimulated for 30 min by manipulating the needle for 30 s every 5 min.The latency and the number of body-writhing response were observed and the contents of β-endorphin(β-EP) in hypothalamus and serum prostaglandin E2(PGE2) were detected with radioimmunoassay(RIA).Results There was no significant difference in the latency and the number of body-writhing response,and contents of hypothalamic β-EP and serum PGE2 between HT and WT control groups(P>0.05).Compared with the corresponding control groups,the latency of body-writhing in WT model and HT model groups shortened significantly and the number of bodywrithing increased considerably in two model groups(P<0.01).While in comparison with WT model group,the latency of bodywrithing prolonged significantly and the number of body-writhing decreased apparently in WT acupuncture group(P<0.01);but no marked differences were found between HT model and HT acupuncture groups in these two indexes(P>0.05).Compared with the corresponding control groups,the contents of both β-EP and PGE2 increased obviously in WT model and HT model groups(P<0.05).In comparison with WT model group,β-EP levels in WT acupuncture group increased further significantly(P<0.05),and serum PGE2 in WT acupuncture group decreased obviously(P<0.05);but no significant changes were found between HT model and HT acupuncture groups in β-EP and PGE2 levels(P>0.05).Conclusion Acupuncture has a marked antinociceptive effect in WT mice with visceral pain,which may be related to its effects in increasing hypothalamic β-EP and decreasing serum PGE2;while in connexin 43 gene knockout mice,all the above-mentioned effects of acupuncture are eliminated,indicating an important role of connexin 43 in the analgesic effect of acupuncture.关键词：Visceral pain;β-endorphin;Prostaglandin E2;Acupuncture;Connexin 43;Gene knockout397|0|2更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) of local plus distal acupoints on spinal substance P expression in chronic radicular pain(CRP) rats so as to investigate its underlying mechanism in the treatment of chronic radical pain.Methods Twenty-five Wistar rats were randomized into control,model,local acupoints [LA,bilateral "Jiaji"(EX-B 2)],distal acupoints [DA,bilateral "Yanglingquan"(GB 34)],and LA+DA groups,with 5 cases in each.CRP model was duplicated by implanting a gel-silicon wrapping the nerve root of L4 under anesthesia.EA(2 Hz,≤2 mA) was applied to EX-B2 and GB34 for 30 min for 8 days.Pathological changes of the local focus tissue were observed by HE dyeing,and the animals' motor state was also observed.The pain threshold was detected by using tail-flick method.Substance P immunoreactive(IR) positive product of the spinal dorsal horn(L3-L5) was detected by immunohistochemical method and expressed as integrated optical density(IOD).Results The motor scores on day 35 after modeling and pain threshold values on day 4 and 8 after treatment in EX-B2 group,GB34 group and EX-B2 +GB34 group were significantly higher than those in model group(P<0.05,0.01).Compared with model group,IOD values of SP in the spinal dorsal horn in EX-B2,GB34 and EX-B2+GB34 groups were significantly lower(P<0.05).There were no significant differences among the 3 EA groups in the expression of SP in the spinal dorsal horn.Conclusion EA of EX-B2,GB34 and EX-B2 +GB34 all has a good analgesic effect in CRP rats,which may be realized partially by suppressing the release of SP in the spinal cord.No significant differences were found among local acupoint,distal acupoint and local plus distal acupoint groups in relieving CRP,improving motor and decreasing SP expression.关键词：Chronic radicular pain;Electroacupuncture;Substance P;Spinal cord331|0|2更新时间：2023-08-11
- 摘要：Objective To observe the effects of manual acupuncture(MA) and electroacupuncture(EA) on Ca2+ content and Ca2+-ATPase activity in sarcoplasmic reticulum of skeletal muscle cells in rats of acute swimming exercise(ASE) so as to reveal its underlying mechanism in strengthening motor abilities.Methods Sixty male SD rats were randomly divided into control,model,MA and EA groups,with 15 cases in each.Rats in MA group and EA group were treated respectively with MA(120-140 twirlings/min,30-60 s/time every 3-5 min) and EA(500-800 Hz,0.20-0.25 mA) stimulation of "Dazhui"(GV 14),"Mingmen"(GV 4),"Huantiao"(GB 30) and "Zusanli"(ST 36) for 20 min before ASE.After ASE(120 min),the rats anesthetized with urethane(1 g/kg) were killed for sampling skeletal muscles(quadriceps muscle of thigh) and for detecting Ca2+ content and Ca2+-ATPase activity with thymol blue chromatometry and chemical chromatometry respectively.Results In comparison with control group,Ca2+ content and Ca2+-ATPase activity in sarcoplasmic reticulum of skeletal muscle cells in model group were obviously lower(P<0.01,0.05);while compared with model group,both Ca2+ content and Ca2+-ATPase activity in sarcoplasmic reticulum in MA and EA groups increased significantly(P<0.01).The activity of Ca2+-ATPase of MA group was evidently higher than that of control group(P<0.05).No significant differences were found between MA and EA groups in Ca2+ content and Ca2+-ATPase activity(P>0.05).Conclusion Both MA and EA can increase Ca2+-ATPase activity and Ca2+ content in sarcoplasmic reticulum of skeletal muscle cells in motor fatigue rats,which may contribute to their effects in strengthening motor ability.359|0|10更新时间：2023-08-11
- 摘要：Objective To explore the relationship between energy metabolism and meridian phenomenon.Methods Ten Boer hybridism goats mitigated under chlorpromazine(0.85 mg/kg,intramuscular injection) were subjected to the present study.Changes of oxygen partial pressure(PO2) in "Ganshu"(BL 18),"Dachangshu"(BL 25) and "Guanyuanshu"(BL 26) of Bladder Meridian and in 3 non-acupoints(3 cm apart from BL18,BL25 and BL26 separately on the unilateral side) were recorded by using a needle-type tissue oxygen tension sensor before and following local injection of EDTA-Na2(200 μL,0.05 kg/mol,for complexing Ca2+) and 0.9% saline.Results ① Compared with the corresponding non-acupoints,Ca2+ potentials in BL18,BL25 and BL26 were all significantly higher(P<0.05,0.01).② In comparison with the corresponding non-acupoints,the values of tissue PO2 of BL18 and BL26 were significantly higher(P<0.01).③ After injection of EDTA-Na2,PO2 values in both BL18,BL26,and their respective non-acupoints increased significantly in comparison with those of either injection of saline or their respective basal values(P<0.01,0.05).Compared with those of their corresponding non-acupoints PO2 values in both BL18 and BL26 increased markedly(P<0.01).Conclusion Ca2+ potential of the Bladder Meridian was significantly higher than that of non-acupoints,and after complexing Ca2+,PO2 in acupoints and non-acupoints increased markedly,suggesting that Ca2+ may play an important role in meridian signaling and production of acupuncture effect.关键词：Oxygen partial pressure;Ca2+;Bladder Meridian301|0|2更新时间：2023-08-11
- 摘要：Objective To observe changes of SP and CCK-8 contents in the hypothalamus and spinal cord after performing acupotomy lysis so as to study its central analgesic effect in rats with transverse-process syndrome of the third lumbar vertebra.Methods Twenty-eight SD rats were randomly divided into normal control group,model group,acupotomy lysis group and electroacupuncture(EA) group,with 7 cases in each one.The model of the third lumbar vertebra transverse process syndrome was established by inserting a piece of gelatin sponge into the deep subfascial part of the 3rd lumbar transverse process.Acupotomy lysis was conducted 1 time/week for 2 weeks.EA(2/100 Hz,1-2 mA) was applied to the left "Shenshu"(BL 23)-"Yaoyangguan"(GV 3) for 20 min/time,3 times/week and for 2 weeks.SP and CCK-8 contents in the hypothalamus and spinal cord were detected with enzyme linked immunosorbent assay(ELISA).Results In model group,SP and CCK-8 contents in the hypothalamus and spinal cord were increased significantly in comparison with normal control group(P<0.01).Compared with model group,both SP and CCK-8 contents in hypothalamus and SP content in spinal cord in acupotomy lysis and EA groups were apparently lower(P<0.01,0.05).CCK-8 content in spinal cord in cupotomy lysis group was lower markedly than that of model group(P<0.01),while no significant difference was found between model group and EA group in CCK-8 level in spinal cord(P>0.05).Conclusion Acupotomy lysis can downregulate SP and CCK-8 contents in hypothalamus and spinal cord,which may contribute to its effect in relieving pain from transverse process syndrome of the third lumbar vertebra.关键词：Transverse process syndrome of the third lumbar vertebra;Electroacupuncture;Acupotomy lysis;SP;CCK;Spinal cord;Hypothalamus361|0|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of transcutaneous acupoint electrical stimulation(TAES) on plasma ET,CGRP and serum IL-6,S100β during craniotomy.Methods Fifty patients scheduled for neurosurgery were randomly divided into TAES group(n=25) and control group(n=25).TAES(2/100 Hz,8-12 mA) of bilateral Hegu(LI 4)-Quchi(LI 11),Zusanli(ST 36)-Sanyinjiao(SP 6) was administrated for patients of TAES group starting 30 min before anesthesia till the end of the operation.Anesthesia of the patients was maintained with sevoflurane inhalation and intermittent intravenous injection of boluses of sulfenany and vecurnium bromide.Jugular venous blood samples were taken at preanesthesia(T0),1 hour after craniotomy(T1),closure complete(T2),24 hours(T3) and 48 hours(T4) after operation respectively for detecting contents of plasma endothelin(ET),calcitonin gene-related peptide(CGRP) and serum interleukin(IL)-6,S100β with radioimmunoassay and enzyme linked immunosorbent assay separately.Results Compared with control group,plasma ET at T2,serum IL-6 at T1,S100β at T1 and T4 in TAES group all decreased significantly(P<0.01,0.05),while serum IL-6 at T3 increased remarkably(P<0.05).It suggests that after TAES,CGRP/ET was improved,favoring cerebral microcirculation to reduce surgery-induced cerebral injury.There were no significant differences between two groups at different time courses in plasma CGRP concentrations(P>0.05).Conclusion TAES can regulate plasma CGRP/ET and serum IL-6,lower serum S100β level,which may contribute to its effectin relieving craniotomy-induced brain injury.关键词：Craniotomy;Transcutaneous acupoint electrical stimulation;Blood bioactive compounds310|1|7更新时间：2023-08-11
- 摘要：This article briefly reviews the general understanding of endogenous neural stem cells(eNSCs) in the CNS over the past 50 years.The proliferation and differentiation of neural progenitors continue throughout the lifetime,instead of cell division stopping at the postnatal period.In the spinal cord,these cells differentiate into different kinds of glial cells.Based on his current research in spinal cord injury(SCI),the author reports that the implantation of functional electrical stimulation(FES) in the motor cortex or the peripheral nerve increases cell birth and differentiation of eNSCs in the spinal cord.Furthermore,electrical stimulation promotes remyelination and neural repair.Finally,this article discusses the possibility of the application of eNSCs and FES in electroacupuncture research.关键词：Neural stem cells;Spinal cord injury;Functional electrical stimulation;Corticospinal tract323|0|8更新时间：2023-08-11
- 摘要：Neurotrophic factors and cytokines are involved in the regulation of neuronal survival,axonal myelination,and synaptic plasticity in both central nervous system(CNS) and peripheral nervous system(PNS).The members of the neurotrophic factor family include nerve growth factor(NGF),brain derived neurotrophic factor(BDNF),neurotrophin-3(NT-3),and neurotrophin-4/5(NT-4/5).These molecules bind to two types of receptors: 1) tyrosine kinase receptors(TrkA,TrkB,TrkC) and 2) common neurotrophin receptor(p 75 NTR).The internalization and retrograde axonal transport of neurotrophin receptors are important for their signal transduction supporting neuronal survival,synaptic plasticity,and axonal myelination.In addition,a growing body of data suggests that neurotrophins are involved in the pathophysiologicl courses of inflammatory pain,neurodegenerative disease,and psychiatric diseases.Cytokines,including IL-1,IL-2,IL-6,and TNF-alpha,are important mediators of the immune response and play a key role in the diseases by acting on inflammatory immune cells,neuronal cells,muscle cells,and vessel cells.Interestingly,some cytokines(e.g.TNF-α,IL-2,TGF-β) are also able to regulate synaptic plasticity and affect CNS functions.The neurotrophins and cytokines release in response to various stimuli,such as electronic stimulation,or inflammation.This crosstalk from PNS to CNS is involved in the pathophysiology of many human diseases and may contribute to the effects of acupuncture.Based on our knowledge to neurotrophins and cytokines,we proposed the neurotrophin/cytokine hypothesis for the mechanism of acupuncture.This hypothesis may initiate the discussion on the possible roles of neurotrophins/cytokines in the therapeutic effects of acupuncture and shed light to the discovery of mechanism of acupuncture in the treatment of devastating diseases.关键词：Acupuncture therapy;nervous system;Neurotrophins;Cytokines;Acupuncture mechanism366|0|4更新时间：2023-08-11
- 摘要：The central nervous system(CNS) is involved in a variety of disease conditions.Some seeming peripheral diseases,like chronic pain and disorders in major organs,indeed have clear pathological basis in the CNS.On the other hand,some clinically-beneficial peripheral stimulation,such as acupuncture and massage,exerts significant influence on central neurons.This review attempts to summary recent findings in neuroscience about how pathological insults long-term plastic changes within neural circuits,leading to maladaptive behaviors.This neuroplasticity-based theory not only conceptualizes a cellular mechanism for a plethora of neuropathophysiology but also provides clinical strategies for treating neural diseases.Drug addiction is a chronic brain disease,defined as compulsive drug-seeking,drug-craving,and drug-taking behaviors.Extensive experimental evidence suggests that following exposure to drugs of abuse,neurons within the mesolimbic dopamine system undergo a series of plastic changes that may lead to compulsive emotional and motivational states.It is believed that the first step to unlock the secret of drug addiction is to identify,evaluate,and conceptualize drug-induced neural plasticity.Synaptic plasticity is one form of neuroplasticity that has been best characterized.Using addiction-related synaptic plasticity as a working model,this review attempts to depict the general concept and experimental approach in studying the pathophysiological neural basis of acupuncture.关键词：Drug addiction;Mesolimbic dopamine system;Neural plasticity;Acupuncture298|0|1更新时间：2023-08-11
- 摘要：We have found that nitric oxide(NO) contents are increased in skin acupoints associated with enhanced neuronal NO synthase(nNOS) protein level in rats.Our recent studies show that L-arginine-derived NO synthesis increases skin electric currents over acupoints in rats.Consistently,norepinephrine(NE) turnover rate is decreased in acupoints with high skin electric currents,and enhanced 3H-NE synthesis/release in acupoints/meridians is facilitated by presence of an exogenous NO donor and inhibited by an inhibitor of NO synthesis.We have developed an innovative method to collect and measure NO metabolites from acupoints and meridians on the skin surface in humans.NO contents are consistently increased over PC and BL acupoints in humans,and increased NO content is reduced by anti-bacteria on skin surface.We conclude that the nonenzymatic reduction of nitrate by bacteria is involved in chemical generation of NO on skin acupoints/meridians in addition to neuronal NOergic system.NO mediates noradrenergic function on skin sympathetic nerve activation,which contributes to low resistance characteristics of acupoints and meridians.332|0|0更新时间：2023-08-11
- 摘要：Acupuncture is recognized as a procedure to regulate functions of human body.In addition to its known effects on nerve system,emerging evidence suggests that acupuncture also delivers its efficacy through innate immune system.Innate immune system includes three major parts,cytokine activity,complement activity,and cell-based activity.Although initially characterized as natural defense mechanism,innate immune system,especially,the Th1/Th2 cytokine/chemokine networks,plays important roles in regulation of many physiological functions including inflammatory/anti-inflammatory activity,pain,metabolism,cell proliferation and apoptosis.Moreover,innate immune system interacts closely with peripheral and central nerve systems.Roles of cytokines/immune cells(macrophages and T cells) in nerve functions and pathogenesis of neuronal diseases have been increasingly recognized.Likewise,CNS through hypothalamic-pituitary-adrenal(HPA) axis and cholinergic anti-inflammatory pathway adjusts immune activity.We present a testable model to validate the effect of acupuncture in innate immune system.The model emphasizes the existence of dynamic cytokine network.The goal is to demonstrate that the combined neuro-immune function is a general mechanism of acupuncture in the treatment of different diseases.关键词：Acupuncture therapy;Innate immunity;Neuro-immune network;Cytokines346|0|3更新时间：2023-08-11
- 摘要：Acupuncture has been widely used in the West in recent years and demand has been growing for scientific evaluation of its clinical efficacy.The practice of evidence-based medicine has brought new challenges in the design of acupuncture research,and publication of randomized clinical trials on acupuncture has significantly increased.While systematic reviews of these trials have advanced our current knowledge,they have exposed deficiencies in research design and revealed that one design can not answer all research questions.Few clinical studies conducted in China have been published in the West,and most published in Chinese suffer from methodological design flaws that render the results unreliable and unconvincing.Such flaws include inadequate or no randomization,inadequate control, unsatisfactory outcome measurements,lack of proper concealment,insufficient follow-up,and improper statistical analysis.To foster high quality acupuncture clinical research in China,we must cultivate innovation and creativity in research design.It is unwise to simply follow or copy the research methodology of Western pharmaceutical studies.Acupuncture and traditional Chinese medicine(TCM) must be evaluated using rigorous scientific methods that preserve the essence of TCM concepts,so that acupuncture and TCM,these ancient healing arts,can continue to play an important role in the health care systems of modern societies.关键词：Acupuncture;Evidence-based medicine;Systematic review;Research methodology of clinical trials332|0|14更新时间：2023-08-11
- 摘要：To evaluate the efficacy of acupuncture scientifically and objectively is a major issue for the development of acupuncture medicine.Although some progresses have already been gained in the research of the evaluation of clinical therapeutic effects of acupuncture at present,some problems as empirical evaluation method,following evaluation indexes of Western medicine,poorer quality of evaluation studies,etc.still exist.Thus,it is urgent to establish a reasonable evaluating system for acupuncture clinical efficacy which accords with the theory of acupuncture,embodies the characteristics and advantages of acupuncture clinic,perfecting the clinical evaluation method,and improving the quality of clinical efficacy evaluation and accelerating the sustainable development of acupuncture clinic.Through reasonable analysis on the current situations about the clinical evaluation research of acupuncture and combining some new concepts and methods,the authors of the present paper hold that the new evaluation system should embody the characteristic of the theory and clinical practice of acupuncture and fully make use of the methods of evidence-based medicine and some measuring scales.They also advocate trying to establish an "evidence-based-goal-attainment scale" method by combining standardized population evaluation with individualized diagnosis-treatment evaluation so as to enhance the international development of acupuncture.关键词：Acu-moxibustion;Clinical efficacy evaluation;Evidence-based medicine;Goal Attainment Scale376|0|5更新时间：2023-08-11
- 摘要：Objective To observe the nerve discharge changes responding to stimulation of "Zusanli"(ST 36) by manual acupuncture(MA) and electroacupuncture(EA),so as to reveal the changing regularity of peripheral nerve response.Methods Thirty male SD rats were used in the present study.After anesthetizing with urethane(75 mg/kg) and dissecting a branch of the sciatic nerve innervating ST36 region in the right hind-limb,the ipsilateral ST36 was stimulated with MA(by lifting and thrusting and rotating the acupuncture needle,1 manipulation/s) and EA(5 V,10 V,15 V,and 3 Hz).Discharges of the nerve tract was recorded for 1 min before,during and after MA or EA stimulation respectively,and analyzed in spike counts.Results After lifting,thrusting and rotating the needle,the nerve discharge increased to(74.20±18.92) spikes/s from(42.20±1.98) spikes/s of pre-MA(P<0.05),then turned to(41.40±4.85) spikes/s immediately after cease of MA.After manual lifting and thrusting the needle,the nerve discharge increased to(60.60±10.79) spikes/s from(42.00±3.89) spikes/s before MA(P<0.05),and returned to(45.00±18.10) spikes/s immediately after cease of MA.No changes were found after manually rotating the needle(P>0.05).EA at ST36 by increasing the intensity to 5 V began to elicit similar changes of nerve discharges,and increased significantly in a voltage-dependent manner within 5-15 V.EA at the strength of 15 V could produce a marked increase of nerve discharge,being increased to(64.20±9.36) spikes/s from(42.60±3.87) spikes/s before EA(P<0.05),and then,returned to(48.00±7.55) spikes/s immediately after cease of EA.Conclusion MA of lifting and thrusting,and particularly that of rotating combined with lifting and thrusting at ST36 can significantly potentiate the discharge activity of the sciatic nerve tract,with the response of the latter being more apparent.EA of ST36 at the strength of 10-15 V has a similar effect.It suggests that both mechanical and EA stimulation of ST36 at a certain intensity can produce a similar change of discharges of the afferent nerve innervating ST36 in the rat.关键词：Acupuncture;Electroacupuncture;"Zusanli"(ST 36);Discharge of the sciatic nerve335|2|8更新时间：2023-08-11
- Postal code：100700
- Tel：010-64089344,84014607 Email：zcyjbjb@vip.163.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05017590号-8
京公网安备11010802020460号 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0