最新刊期
卷 ， 期 3 ， 2008
- 摘要：Objective To investigate the afferent pathways of "Guanyuan"(CV 4) under normal and pathological states and to observe the effect of electroacupuncture(EA) on them.Methods Twenty-four female SD rats were randomly divided into normal control(NC),NC+EA,ovariectomy(OVX) and OVX+EA groups,with 6 cases in each.Four weeks after OVX,Pseudorabies Virus(PRV,10 μl liquid containing 108 plaque-forming units) was injected into CV4.Thirty minutes after PRV injection,EA(2 Hz,2-3 mA) was applied to CV4 for 30 minutes,once daily for 3 days.Six hours after the third EA treatment,all the rats were perfused transcardially with 4% paraformaldehyde under anesthesia,followed by sampling the brain and spinal cord and cutting into sections(35 μm).Immunohistochemical method(rabbit anti-rat beta-galactosidase) was used to display PRV immunoreactive(PRV-IR) positive neurons.Results 1) After injection of PRV,PRV-IR positive cells widely distributed in the spinal cord(cervical,thoracic and lumbar segments),brain stem(nucleus of solitary tract,cuneate nucleus,gigantocellular reticular nucleus,nucleus of spinal tract of trigeminal nerve,nucleus raphe magnus,locus caeruleus,etc),hypothalamus and cerebral cortex in each group.2) After OVX,apparently fewer PRV-IR positive cells were found in some nuclei as medial septum nucleus(MSN),arcuate nucleus(ARC),diagonal band nucleus(DBN),paraventricular nucleus(PVN) which have close relation with endocrine activity(P<0.05);and rarely seen in ventromedial hypothalamus(VMH) and lateral preoptic area(LPO)(P<0.01).But in OVX+EA group,PRV-IR positive cells in above regions or nuclei increased significantly(P<0.05 or P<0.01).No obvious difference was found between NC and NC+EA groups in the number of PRV-IR positive cells(P>0.05).Conclusion Following OVX,PRV-IR positive cells in neuroendocrine-related nuclei of hypothalamus reduced significantly in number;EA of "Guanyuan"(CV 4) can promote the recovery of these neurons in above mentioned nuclei.关键词："Guanyuan"(CV 4);Pseudorabies virus;Neural tracing;Electroacupuncture319|0|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of high-frequency electroacupuncture(EA) on lipid metabolism in obesity rats.Methods Fifty-one SD rats were randomized into control group(n=13),model group(n=19) and EA group(n=19).Obesity model was duplicated by feeding the rats with high fat forage and subcutaneous injection of 15% glutamate sodium solution.EA(100 Hz,3-5 mA) was applied to "Housanli"(ST 36),"Sanyinjiao"(SP 6),"Guanyuan"(CV 4) and "Zhongwan"(CV 12) for 10 min everyday and for 28 days.Lee's index,body weight,area and the number of the adipose cells around the left kidney were detected.Blood samples were collected for assaying serum triglyceride(TG),total cholesterol(TC) with cholesterol oxidase phenol 4-aminoantipyrine peroxidase(CHOD-PAP) method,glycerol phosphate oxidase-p-aminophenazone(GPO-PAP) methods,high density lipid-cholesterol(HDL-C) with phosphotungstic acid-Mg2+ method,low density lipid-cholesterol(LDL-C) with polyvingel sulfate(PVS) sedimentation method and plasma lipoprotein lipase(LPL) with immunoturbidmetry endpoint method,and serum insulin and leptin contents with radioimmunoassay respectively.Results After modeling,in comparison with control group,Lee's index,serum TG,TC,LDL-C,fat weight,adipose cell area,serum leptin and insulin contents in model group increased considerably(P<0.05,0.01),while serum HDL-C,plasma LPL,adipose cell number decreased significantly(P<0.05,0.01).Following EA,compared with model group,Lee's index,serum TG,TC,LDL-C,fat weight,adipose cell area,serum leptin and insulin levels decreased significantly in EA group(P<0.05,0.01),whereas serum HDL-C,plasma LPL,and adipose cell number increased apparently(P<0.01).Conclusion High frequency EA can effectively improve abnormal lipid metabolism,and reduce fat accumulation in obesity rats,which may be closely associated with its effect in regulating the balance between leptin and insulin levels.关键词：High-frequency electroacupuncture;Obesity;Lipid metabolism;Insulin;Leptin447|0|9更新时间：2023-08-11
- 摘要：Objective To study the interrelation between the therapeutic efficacy of electroacupuncture(EA) and mast cells(MC) in experimental gastric ulcer rats.Methods Thirty Wistar rats were randomly divided into normal control group,model group and EA group,with 10 cases in each.Experimental gastric ulcer model was established by injecting 20% glacial acetic acid(0.05 ml) into the tissue between the muscular layer and the placenta percreta of the gastric anterior wall in the gastric antrum under anesthesia.EA(2/15 Hz,1 mA) was applied to bilateral "Wei"(Stomach,an otopoint) and "Zusanli"(ST 36) for 30 min,once daily for 7 days.The tissues of these two acupoints were sampled to be stained with acetylcholine esterase(AChE) and toluidine blue(TB) respectively(counterstaining) after section(15 μm).The gastric ulcer tissue was stained with H&E method.Results Compared with control group,the number and degranulation ratios of MC in the dermis of "Wei"-point and ST36 region in model group and EA group increased significantly(P<0.01);while in comparison with model group,both the number and the degranulation ratio of MC in EA group decreased considerably(P<0.01).H&E stained tissues showed smooth gastric mucosal membrane in control group,ulcerative injury involving the muscular layer in model group,and mild injury in EA group.Conclusion EA can suppress the degranulation of MC in "Wei"-point and ST36 regions and effectively relieve ulcerative injury in gastric ulcer rats.There exists a certain degree of interrelation among the degranulation ratio of MC,gastric ulcerative injury,and the therapeutic efficacy.关键词：Gastric ulcer;Mast cells;Degranulation;Electroacupuncture;"Wei"-point;"Zusanli"(ST 36)390|0|8更新时间：2023-08-11
- 摘要：Objective To explore the effect of electroacupuncture(EA) in resisting acute cerebral ischemia-reperfusion injury(CI-RI) via anti-oxidation of melatonin(MT).Methods A total of 52 male SD rats were randomized into Sham-operation(sham,n=8),CI-RI(model,n=8),EA(n=8),MT(3.2 mg/Kg,i.p.,n=8),EA+MT(n=8),EA+Luzindole(L,a blocker of MT,1 mg/100 μl/rat,n=6),EA+PD 98059(a blocker of mitogen-activated protein kinase,MAPK,1 mg 100 μl/rat,n=6) groups.EA(3 Hz,1-3 mA,continuous waves) was applied to "Dazhui"(GV 14) and "Baihui"(GV 20) for 30 min.CI-RI model was induced by right middle cerebral artery occlusion(MCAO) for 60 min and reperfusion for 24 h except sham-operation group.MT content of pineal gland tissue was determined by high performance liquid chromatography(HPLC) and neuroethological scores of the rats were evaluated by Kuluz's and Julio's methods.Bax and Bcl-2 gene protein expression of the striate body was detected by immunohistochemistry.Results In comparison with sham group,the number of Bax immunoreactive(IR) positive cells and Bax/Bcl-2 in striate body increased significantly(P<0.001),and neuroethological score and Bcl-2 IR-positive cells decreased considerably in model group(P<0.01).Compared with model group,pineal MT content,neuroethological score and Bcl-2 IR-positive cells in EA group,neuroethological scores in EA+L and EA+P groups,and Bcl-2 expression in MT and EA+MT groups increased considerably(P<0.01,0.001);while Bax IR-positive cells and Bax/Bcl-2 in EA,MT and EA+MT groups decreased very significantly(P<0.001).It showed that EA could obviously increase pineal MT content,improve CI-RI rats' activity score,upregulate Bcl-2 expression and down-regulate Bax expression in striate body.In comparison with simple EA group,neuroethological scores of EA+L and EA+P groups,Bax IR-positive cells and Bax/Bcl-2 of EA+MT group were significant lower(P<0.05,0.01).No significant differences were found between EA+L and EA+P groups in behavior scores,and between MT and EA+MT groups in Bax and Bcl-2 IR-positive cell number and Bax/Bcl-2(P>0.05).Conclusion EA has a good effect of anti-oxygen stress,protecting the brain from ischemic damage directly or indirectly,which may be related to its effect in upregulating the synthesis and release of MT.333|0|2更新时间：2023-08-11
- 摘要：Objective To investigate the underlying mechanism of electroacupuncture(EA) of "Shuigou"(GV 26) and "Baihui"(GV 20),"Ganshu"(BL 18) and "Shenshu"(BL 23) in resisting cerebral ischemia(CI).Methods Sixty male SD rats were randomly divided into pseudo-operation group,model group,GV26-GV20 group,and BL18-BL23 group,with 15 cases in each.CI model was established by middle cerebral artery occlusion(MCAO).EA(2/30 Hz,2 mA) was applied to GV26,GV20,BL18 and BL23 for 30 min every time and for 6 times.Neuroethologic scores of the rats were evaluated,plasma and cerebral endothelin(ET) and calcitonin gene-related peptide(CGRP) contents were determined by radioimmunorassay.Results 1) After MCAO for 72 hours,the rats' neuroethologic score,plasma and cerebral ET contents in model group increased significantly in comparison with those of pseudo-operation group(P<0.05,0.01),and plasma and cerebral CGRP levels decreased considerably(P<0.01).Compared with model group,the neuroethologic scores,cerebral ET contents of GV26-GV20 and BL18-BL23 groups decreased significantly after CI(P<0.01);and plasma and cerebral CGRP levels in two EA groups increased significantly(P<0.05,0.01).No significant differences were found between two EA groups in these 3 indexes(P>0.05).Conclusion EA of GV26-GV20 and BL18-BL23 can improve CI rats' behavior activity,which may be related to its effects in lowering cerebral ET and raising blood and cerebral CGRP levels.关键词：Focal cerebral ischemia;Electroacupuncture;Neuroethologic score;Endothelin;CGRP354|0|5更新时间：2023-08-11
- 摘要：Objective To explore the underlying mechanism of scalp-acupuncture therapy in the treatment of acute cerebral ischemia(ACI) in the rat.Methods A total of 140 SD female rats were randomly assigned to sham-operation(n=20),model(n=60),scalp-acupuncture(SA,n=60) groups,and the later two groups were further divided into 24 h,48 h and 72 h subgroups separately,with 20 cases in each.Among them,70 rats were used for cerebral tissue section,and the other 70 cases for homogenating cerebral tissue.ACI model was established by occlusion of the middle cerebral artery(MCAO) for 1 h and reperfusion.EA(2/100 Hz,2 mA) was applied to bilateral "Dingnie Houxiexian"(MS 7) and "Dingnie Qianxiexian"(MS 6) for 20 min,once daily for 1 d,2 d and 3 d respectively.The rat's neurological severity score(NSS) was assessed before and after EA.Blood and brain tissue were sampled for detecting TNF-α and IL-1β contents respectively with enzyme-linked immunosorbent assay.Haematoxylin-eosine(H&E) staining method was used for displaying the inflammatory cells in the ischemic brain tissue.Results(1) After ACI,NSS at each time-point increased significantly,while compared with model group,NSS of SA group decreased apparently 72 h after ACI(P<0.01).Compared with the corresponding time-points of sham-operation group,the number of inflammatory cells,plasma and cerebral TNF-α and IL-1β contents at 24 h,48 h and 72 h in model group increased considerably(P<0.01,0.05).In comparison with the corresponding time-points of model group,the number of inflammatory cells at 48 h and 72 h,plasma and cerebral TNF-α and IL-1β contents at 72 h in SA group declined significantly(P<0.01).Conclusion Scalp-acupuncture can relieve inflammatory cell infiltration,and reduce plasma and cerebral TNF-α and IL-1β contents in ACI rats,which may contribute to its effect in promoting neurofunctional recovery.关键词：Scalp-acupuncture;Cerebral ischemia/reperfusion injury;Inflammatory response;Tumor necrosis factor-α;Interleukin-1β365|0|6更新时间：2023-08-11
- 摘要：Objective To explore the effect of connexin 43 gene knockout on acupuncture analgesia.Methods Seventy-two wide type(WT) and connexin 43 gene knockout mice were separately and randomly divided into:WT control group,WT model group,WT acupuncture group,heterozygous(HT) control group,HT model group and HT acupuncture group,with 12 cases in each.Visceral pain model was established by intraperitoneal administration of acetic acid."Zhongwan"(CV 12) and bilateral "Zusanli"(ST 36) were punctured with a filiform needle for 30 min and stimulated by manipulating the needle 30 s every 5 min.The expression of c-fos in the spinal dorsal horn was assayed by using RT-PCR and western blot techniques.Results There was no significant difference between HT and WT control mice in relative grey value of spinal c-fos mRNA expression(P>0.05),in which few c-fos mRNA and protein expressed.The expression of c-fos mRNA and protein was increased significantly following intraperitoneal acetic acid injection compared with control groups in both HT and WT mice(P<0.01).And no significant difference was found between HT and WT model groups in c-fos mRNA expression(P>0.05).Compared with WT model group,the expression of both c-fos mRNA and c-fos protein in WT acupuncture group was down-regulated significantly(P<0.01).In comparison with HT model group,the expression of both c-fos mRNA and protein in HT acupuncture group was down-regulated but without statistically significant difference(P>0.05).And the expression of c-fos mRNA and protein in HT acupuncture group was significantly higher than that in WT acupuncture group(P<0.05,0.01).Conclusion Acupuncture has a marked antinociceptive effect in visceral pain mice,and simultaneously suppresses the expression of c-fos mRNA and protein evoked by noxious stimulation in the spinal dorsal horn.Connexin 43 gene knockout may weaken acupuncture analgesia and reduce EA-induced down-regulation of c-fos expression,suggesting an involvement of connexin 43 in the analgesic effect of acupuncture.关键词：Visceral pain;Acupuncture analgesia;c-fos mRNA expression;Connexin 43;Gene knockout363|0|0更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the activity of inducible nitric oxide synthase(iNOS),glutathione peroxidase(GSH-Px) and the content of nitric oxide(NO) in colon tissue of depression rats,so as to reveal its mechanism in protecting colonic tissue under depression.Methods Forty male SD rats were equally randomized into normal,model,medication and EA groups.Depression model was established by using chronic unpredictable mild stress stimulation(forced ice-water swimming,electric shock,tail-clamping,etc) combined with lonely raising for 21 days.At the same time of modeling,Fluoxetine(1.8 mg/kg,intragastric perfusion) was given to the rats of medication group,once daily for 21 days.EA(1.5-2 Hz,1-3 mA) was applied to bilateral"Hegu"(LI 4) and "Taichong"(LR 3) for 20 min,once daily for 21 days.The activity of iNOS,GSH-Px and the content of NO in colon tissue were detected by ultraviolet spectrophotometer method.Results In comparison with normal control group,iNOS activity and NO content in colon tissue increased significantly(P<0.05),and GSH-Px activity decreased considerably(P<0.05) in model group.Whereas compared with model group,iNOS activity and NO content decreased significantly(P<0.05),and GSH-Px activity increased significantly in EA and medication groups.No significant differences were found between EA group and medication group in these 3 indexes(P>0.05).Conclusion EA of "Hegu"(LI 4) and "Taichong"(LR 3) can reduce stress induced increase of both iNOS activity and NO content,and decrease of GSH-Px activity in colon tissue in depression rats,which may contribute to its effect in protecting the digestive tract from injury under depression.关键词：Depression;Electroacupuncture;Colon;iNOS;GSH-Px;NO424|0|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of different emulated acupuncture-needle manipulations on blood pressure and myocardial angiotensin(Ang) Ⅱ content in spontaneously hypertensive rats(SHR),so as to seek a better acupuncture parameter for clinical treatment of hypertension.Methods Thirty-three SHR were randomly divided into mild-stimulation group(n=8),moderate-stimulation group(n=8),heavy-stimulation group(n=8) and model group(n=9).Additionally,other 8 normal rats were made up of control group.Blood pressure(BP) was determined by using a non-invasive BP detection system.Myocardial Ang Ⅱ content was assayed by radioimmunoassay.The emulated acupuncture-needle twirling angles and frequencies of 144°,75 r/min;255°,111 r/min;and 360°,140 r/min in mild,moderate and heavy manipulations were applied to bilateral "Zusanli"(ST 36) and "Taichong"(LR 3) for 3 min,once daily for 3 weeks,with an interval of 2 days between every two weeks.Results Compared with pre-therapy,BP values of both heavy-stimulation and model groups increased significantly(P<0.01);and compared with model group,BP values of mild-stimulation and moderate-stimulation groups decreased significantly after the therapy(P<0.05),but were still higher than that of control group(P<0.05).In addition,myocardial Ang Ⅱ contents of mild-stimulation,moderate-stimulation,heavy-stimulation and model groups were significantly higher than that of control group(P<0.05).No significant differences were found between mild-stimulation and moderate-stimulation groups,and between heavy-stimulation and model groups after therapy in BP,and among mild-stimulation,moderate-stimulation,heavy-stimulation and model groups in myocardial Ang Ⅱ contents(P>0.05).Conclusion Mild and moderate manipulations of ST36 and LR3 have apparent influence on BP,but no apparent influence on myocardial Ang Ⅱ content.关键词：Spontaneous hypertension;Acupuncture manipulations;Blood pressure;Myocardial Ang Ⅱ346|0|8更新时间：2023-08-11
- 摘要：Objective To display the Lung Meridian of Hand-Taiyin at the body surface in a virtual human.Methods After defining the acupoints as the control points,chose suitable function interpolation to compose skeleton curve,actualize surface rendering and to rebuild a tube-like meridian model.Results The rebuilt tubular meridian model had a good visual effect,and clearly showed different anatomic structures of the Lung Meridian of Hand-Taiyin.Conclusion We primarily and successfully accomplish the visualization display of the Lung Meridian of Hand-Taiyin in a virtual human,which affords a data carrier for building a meridian research platform.关键词：Lung Meridian of Hand-Taiyin;Visualization;Virtual human;Tubular modeling318|0|1更新时间：2023-08-11
- 摘要：Nowadays,modern information technology(IT) has been gained great development.How to use the advanced IT to protect,spread and develop Chinese traditional culture of acupuncture is an important topic at the moment.Based on expounding characteristics of modern IT,the present article analyzed its advantages in digitalizing,exhibiting and sharing the rich resources of acu-moxibustion.Particularly,through introduction of concrete examples of "Digital Museum(Gallery) of Acu-moxibustion",this paper may provide a useful enlightenment for digitalizing construction of acu-moxibustion resources and promoting the further spread and development of acu-moxibustion learning by utilizing modern IT.关键词：Modern information technology;Acu-moxibustion;Spread and development293|0|1更新时间：2023-08-11
- 摘要：Objective To observe the clinical effect of bee-sting(venom) therapy in the treatment of rheumatoid arthritis(RA).Methods One hundred RA patients were randomly divided into medication(control) group and bee-venom group,with 50 cases in each.Patients of control group were treated with oral administration of Methotrexate(MTX,7.5 mg/w),Sulfasalazine(0.5 g,t.i.d.),Meloxicam(Mobic,7.5 mg,b.i.d.);and those of bee-venom group treated with Bee-sting of Ashi-points and the above-mentioned Western medicines.Ashi-points were selected according to the position of RA and used as the main acupoints,supplemented with other acupoints according to syndrome differentiation.The treatment was given once every other day and all the treatments lasted for 3 months.Results Compared with pre-treatment,scores of joint swelling degree,joint activity,pain,and pressing pain,joint-swelling number,grasp force,15 m-walking duration,morning stiff duration in bee-venom group and medication group were improved significantly(P<0.05,0.01).Comparison between two groups showed that after the therapy,scores of joint swelling,pain and pressing pain,joint-swelling number and morning stiff duration,and the doses of the administered MTX and Mobic in bee-venom group were all significantly lower than those in medication group(P<0.05,0.01);whereas the grasp force in been-venom group was markedly higher than that in medication group(P<0.05).In addition,the relapse rate of bee-venom group was obviously lower than that of medication group(P<0.05;12% vs 32%).Conclusion Combined application of bee-venom therapy and medication is superior to simple use of medication in relieving RA,and when bee-sting therapy used,the commonly-taken doses of western medicines may be reduced,and the relapse rate gets lower.关键词：Rheumatoid arthritis;Bee-sting;Medication254|0|9更新时间：2023-08-11
- 摘要：Objective To compare the therapeutic effects of evening-electroacupuncture(EA) and morning-EA for insomnia.Methods Sixty cases of simple insomnia patients were randomly divided into evening-EA group and morning-EA group,with 30 cases in each.EA(2/100 Hz,1-3 mA) was applied to Baihui(GV 20),Shenting(GV 24),Touwei(ST 8),Shenmen(HT 7),Sanyinjiao(SP 6),etc.in the evening(8:00-9:00 pm) and in the morning(8:00—11:00 am) respectively in two groups,once daily for 10 times.Changes of mean global Pittsburgh Sleep Quality Index(PSQI),sleep rate,frequency of hypnotic-taking,etc were observed before and after the treatment.Results In comparison with pre-treatment,mean PSQI scores in evening-EA and morning-EA groups,and hypnotic-taking frequency in evening-EA group decreased evidently during two weeks after the treatment,and sleep rates of two groups increased significantly from the 1st day on after the treatment in evening-EA group and from the 1st week on in morning-EA group(P< 0.01).Comparison between two groups showed that the mean PSQI scores at the 1st and 2nd week in evening-EA group were significantly lower,and the sleep rate in evening-EA group on the 2nd week after the treatment was considerably higher than that in morning-EA group(P<0.05).Conclusion Evening-EA can significantly lower insomnia patients' PSQI score,raise sleep rate,and reduce the hypnotic-taking frequency.Evening-EA is superior to morning-EA in the treatment of insomnia.关键词：Insomnia;Electroacupuncture;Treatment opportunity295|0|4更新时间：2023-08-11
- 摘要：Qualitative research refers to a generic name of research methods corresponding to the quantitative research.It is different from quantitative research not only in methods,properties of the collected data,but also and particularly in philosophy,ideology and epistemology.In the present paper,the authors make a brief introduction about the background and methodology of qualitative research,and forecast the future possible tendency of the combined clinical trails of acu-moxibustion.Hopefully,it may provide a possible model for acupuncture clinical research methods and make the acupuncture researchers understand the qualitative research methods in clinical practice so as to promote the further development of acupuncture clinical trails,improve the level of acupuncture theory and provide substantial acupuncture clinical evidence.关键词：Acupuncture therapy;Clinical trails;Qualitative research340|0|0更新时间：2023-08-11
- 摘要：By ways of favorable bi-directionally-regulative effect of acupoint stimulation and close correlation between acupoints and Zangfu-organs,acupuncture therapy can effectively regulate both blood pressure and functional activities of the corresponding viscera.Hence,acupuncture therapy,as a complementary method for hypertension,has its characteristics in protecting target organs.In the present paper,the authors review the progress in acupuncture treatment of hypertension in recent ten years from the studies of clinical treatment and animal experiments;analyze the underlying mechanism of acupuncture in protecting target organs of hypertension from the neuro-endocrine-immune network.Moreover,the authors point out some existing problems and put forward a few suggestions about acupuncture research in this field.关键词：Acupuncture;Hypertension;Target organ;Neuroendocrine-immunological net;Review353|0|3更新时间：2023-08-11
- 摘要：Most acupuncture points correspond to low skin-resistance points(LSRP) on the body surface along the meridians.We did three experiments which conformed that the skin nitric oxide(NO) concentration and expression of neuronal nitric oxide synthesis(nNOS) were higher than those in non-acupoints,and non-meridian control points.Noradrenaline(NE) synthesis/release was modulated by exogenous NO donor and selective inhibitor of nNOS in the skin acupoints/meridians.Skin electrical currents in low skin resistance points were modified by L-arginine-derived NO synthesis and NE.As a conclusion,NO-NE contribute to low resistance characteristics of acupoints and meridians.关键词：Low skin resistance points;Nitric oxide;Noradrenaline347|0|6更新时间：2023-08-11
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