最新刊期
卷 33 ， 期 6 ， 2008
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- 摘要：Objective To observe the effect of acupuncture on dysmenorrhea response of Cx43 knock out mice.Methods Two months old heterozygote [Cx43(+/-)] type and wild type [Cx43(+/+)] female mice(20 mice/type)were allocated into normal group,model group,acupuncture group and Yimucao Gao(Extractum Leonuri Inspissatum)group randomly.Dysmenorrheal model was established by feeding the mouse with diethylstilbestrol(2 mg/g)once daily for 12 days.Ocytocin(i.p.2 U/kg)was injected on the 12th day 1 h later after the feeding.Acupuncture of "Sanyinjiao"(SP 6)and "Diji"(SP 8)was given to mice of acupuncture group,once daily for 5 days from the 7th day on after modeling.The latency of body-writhing and the writhing number were measured.Ocytocin receptor(Oct-R)mRNA and vasopressin receptor(Vas-R)mRNA expression in the uterus tissue was detected by using reverse transcription polymerase chain reaction(RT-PCR)technique,and Oct-R expression was detected with immunohistochemistry after fixating and sectioning the uterus tissue.Results In comparison with their individual normal group,the latency of body-writhing of model groups in Cx43(+/+)and Cx43(+/-)mice shortened considerably,while the writhing number increased significantly(P<0.01).Compared with the corresponding model group,the writhing latencies of acupuncture and Yimucao Gao groups in Cx43(+/+)mice and that of Yimucao Gao group in Cx43(+/-)mice increased remarkably,and the writhing numbers of acupuncture and Yimucao Gao groups in Cx43(+/+)mice and that of Yimucao Gao group in Cx43(+/-)mice reduced markedly(P<0.05).In Cx43(+/+)and Cx43(+/-)mice,compared with their individual normal group,Oct-R and Vas-R mRNA expressions and Oct-R expression in model group were upregulated obviously(P<0.05).Compared with the corresponding model group,Oct-R and Vas-R mRNA expressions and Oct-R expression of acupuncture and Yimucao Gao groups in Cx43(+/+)mice,and those of Yimucao Gao group in Cx43(+/-)mice were down-regula-ted significantly(P<0.05).No significant differences were found between acupuncture and corresponding model groups in Cx43 ted significantly(P<0.05).No significant differences were found between acupuncture and corresponding model groups in Cx43(+/-)mice in the above-mentioned indexes(P>0.05).Conclusion Acupuncture can inhibit diethylstilbestrol +ocytocin induced body writhing response in Cx43(+/+)mice rather than in Cx43(+/-)mice,which is closely related to its effects in down-regulating Oct-R mRNA,Vas-R mRNA and Oct-R expression.1208|0|0更新时间：2023-08-11
- 摘要：Objective To investigate the effects of electroacupuncture(EA)on the expression of corticotropin releasing hormone(CRH)and CRHR1mRNA in paraventricular nucleus(PVN)of hypothalamus,to explore its mechanism in anti-anxiety.Methods Thirty-three male SD rats were randomly divided into control,model and EA groups.Chronic emotional stress anxiety model was established by using chronic unpredictable emotional stress stimulation for 21 days.EA(15/25 Hz,1-2 mA)was applied to "Baihui"(GV 20)and "Sanyinjiao"(SP 6)for 15 min/d and 21 days.The expression of CRH and CRHR1mRNA in hypothalamic PVN was measured by immunohistochemistry and in situ hybridization techniques separately.Results Compared with control group,the values of open-arms entries(OE%)and open-arms time(OT%)in model group were decreased significantly(P<0.01).In comparison with model group,OE% and OT% in EA group were increased obviously(P<0.05,P<0.01).Compared with control group,the optical density values of CRH immuno-reaction positive products and CRHR1mRNA expressed neurons of hypothalamic PVN in model group were increased obviously(P<0.05,P<0.01);while in comparison with model group,those of CRH and CRHR1mRNA in EA group were significantly decreased(P<0.05,P<0.01).Conclusion EA can effectively relieve chronic stress stimulation induced anxiety in the rat,which is closely related to its effect in down regulating both CRH and CRHR1mRNA expression in PVN of hypothalamus.关键词：Electroacupuncture;Anxiety;Hypothalamic PVN;CRH;CRHR1 mRNA1228|0|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)on hippocampal apoptosis and learning-memory ability in vascular dementia(VD)mice so as to investigate its underlying mechanism in the treatment of VD patients.Methods Kunming mice were randomly divided into sham-operation(sham,n=13),model(n=15),EA(n=16)and Nimodipine(intragastric gavage,30 mg/kg for 15 days,n=15)groups.VD model was duplicated by occlusion of bilateral carotid arteries and reperfusion.EA(2-80 Hz,2 mA)was applied to "Dazhui"(GV 14),"Baihui"(GV 20),"Geshu"(BL 17)and "Zusanli"(ST 36)for 10 min,once daily for 15 days.Step-down and step-up tests were performed to assess the animal's memory and learning abilities separately;and the terminal deoxynucleotidyl transferase-mediated dUTP-nick end-labeling(TUNEL)method was used to display the apoptotic cells of the hippocampus tissue.Results In comparison with sham group,the animals' reaction time upon electric shock stimulation,both step-up and step-down error times,and hippocampal apoptosis number in model,EA and Nimodipine groups increased significantly(P<0.01);while the reaction latency upon electric shock of model,EA and Nimodipine groups decreased significantly(P<0.01).Compared with model group,the reaction time,both step-up and step-down error times and hippocampal apoptosis number of both EA and Nimodipine groups decreased significantly(P<0.05,P<0.01);while the reaction latency of EA and Nimodipine groups increased considerably(P<0.01).Comparison between EA and Nimodipine groups showed that the reaction latency of EA group was obviously longer than that of the later group(P<0.01),and the step-down error times of EA group was markedly lower than that of Nimodipine group(P<0.05).No significant differences were found between these two groups in other indexes(P>0.05).Conclusion EA can ameliorate VD mice's learning-memory abi-lity,which may be closely related to its effect in reducing hippocampal apoptosis.关键词：Electroacupuncture;Vascular dementia;Apoptosis;Learning-memory ability1225|0|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)on hippocampal apoptosis protein caspase-9 expression and neuroethology in hyperlipemia plus cerebral ischemia(HL-CI)rats.Methods Seventy male SD rats were randomized into control,hyperlipemia(HL),CI,HL-CI,CI+EA,HL-CI+EAⅠ,and HL-CI+EA Ⅱgroups,with 10 cases in each.HL model was established by feeding the animals with high fat forage for 6 weeks and CI model was established by occlusion of the unilateral middle cerebral artery.EA(1-3 mA,15 Hz)was applied to bilateral "Sanyinjiao"(SP 6)and "Fenglong"(ST 40)for 20 min every time;and "Baihui"(GV 20)and "Shuigou"(GV 26)were punctured and stimulated by twirling the acupuncture needle with hand continuously for 1 min.Acupuncture was given once daily for 17 days(beginning from the 10th day on before CI)in HL-CI+EA Ⅰ group,and for 7 days(beginning after CI)in HL-CI+EA Ⅱ group.The expression of hippocampal Caspase-9 was detected with immunohistochemistry.The score of neuroethology was also measured according to modified Bederson's method.Results In comparison with normal control and hyperlipemia model groups,Caspase-9 immune reaction(IR)positive cells in the hippocampus in HL-CI group increased significantly(P<0.01).After acupuncture Caspase-9 IR-positive cells decreased remarkably.In comparison with group HL-CI+EA Ⅱ,Caspase-9 IR-positive cells decreased significantly in HL-CI+EA Ⅰ(P<0.01).The score of neuroethology also degraded obviously.Conclusion Acupuncture can improve neuroethology symptom,lessen over expression of hippocampal Caspase-9,and prevent CI injury in hyperlipemia rats with concurrent CI.关键词：Hyperlipemia with cerebral ischemia;Acupuncture;Caspase-9 expression;Hippocampus1085|0|4更新时间：2023-08-11
- 摘要：Objective To explore the afferent pathway of signals from electroacupuncture(EA)at "Sibai"(ST 2)acupoint.Methods A total of 36 SD rats were randomly divided into control,sham operation(sham),capsaicin,vehicle,capsaicin+EA,and vehicle+EA groups,with 6 cases in each.EA(1/20 Hz,1-3 mA)was applied to "Sibai"(ST 2).After anesthesia(10% urethane),unilateral infraorbital nerve was separated to be immersed in a 1.5% capsaicin-fluid-soaked cotton ball for lesion of C fibers.Vehicle(20% tween)was used to immerse infraorbital nerve for vehicle and vechile+EA groups.The expression of c-fos in the caudal spinal trigeminal nucleus(cSTN)was detected by immunohistochemistry.Results In control group,c-fos immune-reaction(IR)positive neurons were observed to scatter in every layer of cSTN.No significant differences were found among vehicle,capsaicin,sham and control groups in the number of c-fos IR positive neurons in cSTN.The number of c-fos positive neurons in theⅠ-Ⅱ layers of cSTN was obviously increased following EA at ST2,being significant more than that in control,vehicle,capsaicin and sham groups(P<0.01 respectively).However,the number of c-fos IR-positive neurons in Ⅲ-Ⅳ layers of cSTN had no marked change.In capsaicin+EA group,the number of c-fos-IR positive neurons in theⅠ-Ⅱ layers of cSTN was obviously less than that in vehicle and vehicle+EA groups(P<0.01),but no significant changes were found in Ⅲ-Ⅳ layers of cSTN.Conclusion C afferent fibers of the infraorbital nerve are the primary afferent pathway of EA signals from Sibai(ST 2)stimulation in the rat.关键词：c-fos expression;Electroacupuncture;Caudal spinal trigeminal nucleus;C-afferent fibers1271|0|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)on inositol triphosphate(IP3)and diacylglycerol(DAG)contents in cerebral arteries of the cerebral cortex in rats with cerebral ischemia(CI),so as to explore its effect on intracellular signal transduction pathway.Methods Fifty-six Wistar rats were randomly divided into control(n=8),CI(n=24)and EA(n=24)groups,and the later 2 groups were further separately divided into 1 h,3 h and 6 h subgroups(CI-1 h,CI-3 h,CI-6 h,EA-CI-1 h,EA-CI-3 h and EA-CI-6 h),with 8 cases in each.CI model was made by occlusion of the middle cerebral artery.EA(15 Hz,0.1 mA)was applied to "Shuigou"(GV 26)for 20 min.The cerebral arteries in the cerebral shallow layer were collected for assaying intracellular DAG content by thin-layer chromatography(TLC)and IP3 content by competitive protein binding assay(CPBA).Results Compared with control group,the levels of IP3 and DAG in cerebral vascular smooth muscle were significantly increased in model 1 h,3 h and 6 h subgroups(P<0.01).Compared with the corresponding model subgroups,the levels of IP3 and DAG were significantly decreased in EA-CI-1 h,EA-CI-3 h and EA-CI-6 h subgroups(P<0.01).Conclusion EA of GV 26 can effectively inhibit CI-induced increase of intracellular IP3 and DAG levels in rat cerebral arteries,which may contribute to its effect in modulating vascular motor to ameliorate cerebral microcirculation.关键词：Cerebral ischemia;Electroacupuncture;Cerebral inositol triphosphate;Diacylglycerol971|0|6更新时间：2023-08-11
- 摘要：Objective To investigate the effect of manual acupuncture on long-term potentiation(LTP)in CA1 area in hippocampal slices from rats suffered from diabetes mellitus(DM)and cerebral ischemia(CI).Methods A total of 54 female Wistar rats were randomly divided into 5 groups:normal control(Con),CI,DM+Sham-CI,DM+CI and DM+CI+acupuncture(Acup).DM model was established by i.p.of streptozotocin(60 mg/kg),and CI model duplicated by bilateral common carotid arteries occlusion and reperfusion."Baihui"(GV 20),bilateral "Sanyinjiao"(SP 6)and "Pishu"(BL 20)or "Baihui"(GV 20),bilateral "Shenshu"(BL 23)and "Zusanli"(ST 36)were punctured alternatively and stimulated manually,once every other day for 15 times.Hippocampal slices were taken 40 days after modeling.LTP in hippocampal CA1 induced by high frequency stimulation(100 Hz,100 pulses)of Schaffer collaterals and population spikes(PS)were recorded by using glass micropipettes.Results No significant differences were found between DM+CI and normal control groups in the shape and amplitude of PS(P>0.05).After stimulation of Schaffer collaterals,the amplitude and slope rate of PS in hippocampal CA1 of DM+CI rats were decreased significantly in comparison with normal control group(P<0.05,P<0.01).While compared with DM+CI group,both the amplitude and slope rate of PSin DM+CI +Acup groupincreased significantly(P<0.05,P<0.01),suggesting basic restoration of LTP after acupuncture.Conclusion Acupuncture can promotethe recovery of DM+CI induced decline of the amplitude and slope rate of PS,which may contributetoits effect ini mproving memory abilityin DM+CI rats through regulating hippocampal neuronal synaptic plasticity.关键词：Diabetes mellitus;Cerebral ischemia;Acupuncture;Long-term potentiation;Hippocampus;Learning-memory1094|0|4更新时间：2023-08-11
- 摘要：Objective To observe changes of the partial oxygen pressure(PO2)of deep tissues along the Governor Vessel and during acupuncture,so as to probe into the characteristics of energy metabolism in the deep tissues along the meridian track.Methods Thirty-one healthy volunteers were enrolled in the present study.PO2 values in Zhongshu(GV 7),Zhiyang(GV 9)and Mingmen(GV 4)of the Governor Vessel,and 2 non-acupoints(below the 4th and the 12th vertebrae respectively)were detected by using a three-channels needle-type sensor inserted into the tissues about 1.5 cm deep under the skin.The detection was conducted continuously for 60 minutes before,during and after electroacupuncture(EA,2 Hz,20 min)stimulation of Yaoyangguan(GV 3).Results At normal state(before EA),PO2 values were(10.98±5.81)kPa in Governor Vessel(GV 4,GV 7 and GV 9),being significantly higher than those [(5.00±3.88)kPa,(4.74±4.23)kPa] of the left and the right control points separately.During acupuncture of GV3,PO2 value [(9.36±5.99)kPa] in Governor Vessel decreased significantly in comparison with that of pre-EA;while those [(5.83±4.75)kPa and(5.58±5.28)kPa] in the left and the right control points had no obvious changes(P>0.05).And after acupuncture,the reading of PO2 was(8.62±5.11)kPa along the Governor Vessel being comparable to that of EA-period,and those [(5.74±4.76)kPa and(5.61±5.45)kPa] in the left and the right control points still had no obvious changes.Conclusion PO2 in the deep tissues along the Governor Vessel is significantly higher than those of its bilateral control points,and decreases during and after EA,displaying a more vigorous energy metabolism in the deep tissues along the Governor Vessel in normal state,and up-regulation after EA.关键词：Governor Vessel;Oxygen partial pressure;Energy metabolism1251|0|7更新时间：2023-08-11
- 摘要：Objective To observe the curative effect of acne conglobata treated by encircling acupuncture combined with ventouse and cupping.Methods A total of 52 acne conglobata patients were randomly divided into acupuncture group(n=26)and Western medicine group(n=26).Patients of acupuncture group were treated with encircling acupuncture around the affected focus.Common acupuncture was applied to Hegu(LI 4),Xuehai(SP 10),Fenglong(ST 40)and Sanyinjiao(SP 6),once daily.Dazhui(GV 14)and Feishu(BL 13)were used for venesection and cupping(twice a week).Patients of medication group were treated with oral administration of Isotretinoin Capsules(10 mg,t.i.d.).The treatment duration of 2 groups was 4 weeks.Serum IL-6 content was detected with double-antibody sandwich elisa enzyme linked immunosorbent assay(ELISA).Results After the treatment,in acupuncture group and Western medicine group,3(11.5%)and 4(15.4%)cases experienced remarkable relief in their signs,14(53.8%)and 11(42.3%)had marked improvement,6(23.1%)and 7(26.9%)had improvement,3(11.5%)and 4(15.4%)failed,with the effective rates being 88.5% and 84.6%,respectively.No significant difference was found between two groups in the therapeutic effect(P>0.05).Self-comparison showed that after the treatment,IL-6 in both groups decreased significantly(P<0.01).The therapeutic effect of acupuncture group was significantly superior to that of Western medicine group in lowering serum IL-6(P<0.05).Conclusion Both acupuncture and medication can effectively promote the recovery of the affected skin,and lower serum IL-6 level in acne conglobata patients.The effect of acupuncture is stronger than that of Isotretinoin Capsules in lowering serum IL-6 content and has fewer adverse effects.关键词：Acne conglobata;Encircling acupuncture;Venesection and cupping;Serum IL-61065|0|1更新时间：2023-08-11
- 摘要：Objective To explore the mechanism of ginger-partitioned moxibustion in the treatment of cold-damp stagnation type primary dysmenorrhea(PD)patients.Methods A total of 209 PD outpatients of cold-damp stagnation type from 3 hospitals were randomized into moxibustion group(n=105)who were treated with ginger-partitioned moxibustion,and control group(n=104)who were asked to take Yueyue Shu Granules(an effective patent drug for PD).Ginger-partitioned moxibustion was applied to Shenque(CV 8)and Guanyuan(CV 4)from the menstrual onset on for the first course and 3 days before the onset for the second and third course,continuously for 3 days.Before and after the treatment,plasma endothelin-1(ET-1)and serum nitric oxide(NO)contents in those patients(n=40/group)and normal women(n=20)experiencing menstrual period were determined by radioimmunoassay and nitrate reductase method.Results After the treatment,of the 105 and 104 cases in moxibustion and control groups,58 and 32 cured,37 and 33 markedly effective,5 and 24 effective,5 and 15 failed,with the effective rates being 95.24% and 85.58% respectively.The therapeutic effect of moxibustion group was significantly better than that of control group(P<0.05).Before treatment,in comparison with normal group,plasma ET-1 contents in both moxibustion and control groups were significantly higher(P<0.01),while serum NO contents in these two groups markedly lower(P<0.01).After the treatment,self-comparison of both moxibustion and control groups showed that plasma ET-1 decreased significantly and serum NO levels in increased considerably(P<0.05,P<0.01),and the therapeutic effect of moxibustion group was markedly superior than that of control group in lowering plasma ET-1(P<0.01).Conclusion Ginger-partitioned moxibustion is effective in relieving primary dysmenorrhea patients' abdominal pain,which may be closely associated with its actions in lowering plasma ET-1 level and raising serum NO contents.关键词：Primary dysmenorrhea;Ginger-partitioned moxibustion;Plasma ET-1;Serum NO1179|0|6更新时间：2023-08-11
- 摘要：Systems biology,a major science characterized by holistic view,will give a far-reaching impact on our understanding about the essence of biosystem.In the present study,the authors analyze the methodological characters of the systems biology,its relation to and its possible impact on acu-moxibustion medicine.Up to now,modern studies have revealed only partial content of traditional Chinese medicine including acu-moxibustion medicine.With the help of methods,techniques and research thoughts of systems biology,the mechanism of basic theory and clinical efficacy of acupuncture and moxibustion will be understood thoroughly and more rapidly.关键词：Systems biology;Acu-moxibustion;Modernization825|0|5更新时间：2023-08-11
- 摘要：An increasing evidence shows that in the treatment of depression,acupuncture therapy is definitely effective.It has fewer side-effects and is extensively applicable for different types of patients.In the present paper,the authors follow the methodology of evidence-based medicine to make a systemic summary about acupuncture treatment of depression from 1)different therapeutic methods,such as electroacupuncture,acupuncture combined with psychological comforts,acupuncture combined with medication,etc.2)different states of disease,and 3)special disease-induced psychological disorders.Moreover,the authors also make a discussion and a prospect on the treatment of depression and put forward some suggestions for clinical researches in the coming days.关键词：Depression;Acupuncture therapy;Treatment methods1075|0|13更新时间：2023-08-11
- 摘要：The "structure imperfectness of signal channel rule" put forward by the author of the present paper may provide a theoretical evidence for the systematicness of meridian information channel.The conclusion that no special structure of the meridian-collateral system has been found is likely to serve as a piece of counterevidence.According to the latest structural view,the development of capillaries,lymphatic vessels and nerves needs target cells-released inducible factors.In the initial phase of the development of the organism,the asymmetry of the interspaces among cells results in the production and arrangement imbalance of the sequential factors which make the capillaries,lymphatic vessels and nerves distribute sequentially in time and space.Meridian-collateral,following the "systemic statistic distribution rule",is a general expression of this distribution pattern.As a systematic structure,the meridian-collateral system distributes in an optimized way in the human body and has both orderly and compatible characteristics.The author thinks that the meridian-collateral information channel is anatomically visible in the time and spatial structure,and in its logical structure and compatibility during the process of growth.Hence,many techniques of delicate anatomy,quantitative anatomy,growth anatomy,comparative anatomy,tridimensional remodeling of living creature and dynamical remodeling of growth all should be used as the important tools for studying the meridian information channel.The theory and the anatomical techniques determine what you would finally find.关键词：Meridian-collateral;Information-channel structure;Anatomical structure;Theoretical study999|0|0更新时间：2023-08-11
- 摘要：Today,when acupuncture and surgery have been combined for a half century,the author of the present paper holds that the main role of acupuncture in whole process of the surgery does not only mean anesthesia.Getting this point consciously,the author points out that acupuncture will not only have a bigger role in the operating room,but also plays an important and far-reaching impact on the acupuncturology and even the whole life science.关键词：Acupuncture anesthesia;Acupoint-injection;acupoint-immune;acupuncture pretreatment;Research development960|0|4更新时间：2023-08-11
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