最新刊期

    35 3 2010
    • ZHANG Yan, MA Shu-lan, CHEN Bo-ying, TIAN Zhan-zhuang
      Vol. 35, Issue 3, Pages: 163-169(2010) DOI: 10.13702/j.1000-0607.2010.03.001
      摘要:Objective To observe the effects of electroacupunture (EA) on the abnormal hypothalamus-pituitary-adrenal (HPA) axis and the expression of hypothalamic corticotropin-releasing factor (CRF) peptides family and CRF receptor (R)1 mRNA in traumatized rats,so as to study its underlying mechanism in improving traumatic injury. Methods Forty male Sprague-Dawley rats were randomly divided into normal control group (n=10),normal+ EA group (n=10),surgical trauma (model) group (n=10),and model+EA group (n=10). Traumatic injury model was established by performing an exploratory laparotomy in the rats under anesthesia. EA (2 Hz/15 Hz,1-2 mA) was administered for 30 min via two stainless steel needles inserted in "Zusanli" (ST 36) and "Sanyinjiao" (SP 6). Serum adrenocorticotropic hormone (ACTH) ,corticosterone (Cort),luteinizing hormone (LH) and testosterone (T) contents were detected by double-antibody radioimmnoassay. Hypothalamic CRF and CRF R1 mRNA expression was assayed by reversed transcription polymerase chain reaction (RT-PCR) technique. Results The serum ACTH concentration decreased significantly in model group (P<0.05) compared with those in normal control and normal+EA groups,and was significantly higher in model+ EA group than that in model group (P<0.05). No significant difference was found between normal control and normal+EA groups in serum ACTH levels (P>0.05). The serum Cort level increased significantly in model group(P<0.05) compared with those in normal control and normal+EA groups,and was obviously lower in mo-del+EA group than that in model group (P<0.05). While serum LH and T contents had no apparent changes in the four groups (P>0.05). The expression of hypothalamic CRF mRNA decreased significantly in model group (P<0.05) compared with that in normal control group,but increased significantly in model+EA group (P<0.05) compared with that in model group. However,no significant difference was found between normal control and normal+EA groups in the CRF mRNA expression level. The expression of hypothalamic Ucn 1 mRNA increased significantly in model group than that in normal control group (P<0.05),and decreased considerably in model+ EA group than that in model group (P<0.05). There were no significant changes in the expression of hypothalamic Ucn 2 mRNA,Ucn 3 mRNA and CRF R1 mRNA in the four groups (P>0.05). Conclusion EA of "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) can improve surgical trauma-induced HPA disorders,which is probably related to its effects in upregulating CRF mRNA expression and downregulating Ucn 1 mRNA expression in the hypothalamus.  
      关键词:Trauma;Electroacupuncture;HPA axis;CRF;Ucn 1;Ucn 2;Ucn 3;CRF R1   
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    • CHEN Yi-guo, LI Chun-ri, SONG Yuan-yuan, ZHENG Jie
      Vol. 35, Issue 3, Pages: 170-174(2010) DOI: 10.13702/j.1000-0607.2010.03.002
      摘要:Objective To observe the changes of renal protein expression after electroacupuncture (EA) of "Taixi" (KI 3) of the Kidney Meridian of Foot-Shaoyin in healthy rats so as to explore the relationship between KI 3 and the Kidney. Methods Twelve adult male Wistar rats were randomly divided into normal control group (n=6) and EA group (n=6). EA (2 Hz/100 Hz,2-4 mA) was applied to bilateral KI 3 for 20 min,once a day for 7 days. Under anesthesia,the rats were perfused transcardiacally with ice saline and their kidneys removed for extracting the total proteins and assaying the differentially expressed proteins with two-dimensional gel electrophoresis (2-DE),Image MasterTM 2 D Platinum Software and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) MALDI-TOF-MS,respectively. Results Image MasterTM 2 D Platinum Software analysis showed that after EA,9 protein points of the kidney tissues expressed differentially above 3-fold in comparison with control group were upregulated,2 of which were identified to be NAD-dependent isocitrate dehydrogenase and Quinone reductase. No downregulated differentially expressed proteins were found in the kidney tissues. Conclusion EA of "Taixi" (KI 3) can increase the expression of NAD-dependent isocitrate dehydrogenase and Quinone reductase in the kidney tissue,suggesting an increase of energy metabolism after EA and a close correlation between the KI 3 (Source-point) and the Kidney.  
      关键词:Taixi (KI 3);Kidney;Correlation between acupoints and Zangfu organs;Differentially-expressed proteins;Two-dimensional gel electrophoresis   
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    • REN Xiu-jun, MA Hui-fang, TU Ya
      Vol. 35, Issue 3, Pages: 175-181(2010) DOI: 10.13702/j.1000-0607.2010.03.003
      摘要:Objective To observe the effect of electroacupuncture (EA) and acupuncture (A) on the proliferation of stem cells in the subependymal zone (SPZ) of the lateral ventricle and the frontal lobe cortex in hyperlipemia(HL) combined with cerebral ischemia (CI) rats. Methods A total of 72 male SD rats were randomized into control,HL,HL+ EA,CI,CI+A,HL+CI,HL+CI+EAⅠ and HL+CI+EAⅡgroups (n= 9 /group).HL model was established by feeding the animals with high fat forage for 6 weeks and CI model was established by FeCl3-induced occlusion of the unilateral middle cerebral artery. EA was applied to "Sanyinjiao" (SP 6) and "Fenglong" (ST 40) once daily for 17 days for HL+ EA group; and acupuncture to "Baihui" (GV 20) and "Shuigou" (GV 26) once daily for 7 days for CI+A group. For HL+CI+EA Ⅰ group,EA was applied to SP 6+ST 40 first before CI,once daily for 10 days,followed by EA of SP 6+ST 40 and acupuncture of GV 20+GV 26 for 7 days after CI. For HL+CI+EA Ⅱ group,no treatment was given before CI,then,acupuncture of GV 20+GV 26 and EA of SP 6+ST 40 were given once daily for 7 days after CI.The immunoactivity of Nestin and proliferation cell nuclear antigen (PCNA) of SPZ was detected by immunohistochemistry.Results In comparison with normal control group,the numbers of both Nestin and PCNA immunoreaction (IR) positive cells in the dorsolateral extension and the wall of the lateral ventricle of the brain increased significantly in CI and HL+CI groups (P<0.01). Compared with CI group,the numbers of Nestin and PCNA IR positive cells in the dorsolateral extension and the wall of the lateral ventricle in CI+A group increased considerably (P<0.01). In comparison with HL+CI group,both Nestin and PCNA IR positive cell numbers in the dorsolateral extension and the wall of the lateral ventricle of the brain in HL+CI+EA Ⅰand HL+CI+EA Ⅱ groups increased significantly (P<0.01),and the effect of HL+CI+EA Ⅰgroup was markedly superior to that of HL+CI+EA Ⅱ group in upregulating the numbers of Nestin and PCNA IR positive cells in the aforementioned regions of the lateral ventricle (P<0.01). No significant differences were found between HL and control groups,and between HL+EA and HL groups in the numbers of Nestin and PCNA IR positive cells in the dorsolateral extension and the wall of the lateral ventricle of the brain (P>0.05). Conclusion EA can upregulate Nestin and PCNA expression of the dorsolateral extension and the wall of the lateral ventricle of the brain on the ischemic side in rats with CI,and with HL+CI,which may contribute to its effects in promoting the proliferation and migration of neural stem cells in the brain.  
      关键词:Electroacupuncture;Hyperlipemia with cerebral ischemia;Nestin;PCNA;Subependymal zone   
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    • ZHANG Jiang-ling, CHEN Jie, WANG Xiang-rui, LI Wei-wei, WANG Bei-lei, ZHOU Jie
      Vol. 35, Issue 3, Pages: 182-187(2010) DOI: 10.13702/j.1000-0607.2010.03.004
      摘要:Objective To investigate the effects of "Neiguan" (PC 6)-electroacupunture (EA) preconditioning on the myocardium and its mast cells in myocardial ischemia /reperfusion (MI/R) rats. Methods Eighteen male SD rats were randomly assigned to sham group,model (IR) group and EA group (n=6/group). MI/R model was established by occlusion of the descending anterior branch of the coronary artery. Blood samples were taken from the femoral vein before MI (T0),EA for 30 min (T1),30 min after MI (T2),30 min after MI/R (T3) and 120 min after MI/R (T4) for assaying serum tumor necrosis factor (TNF)-α and histamine contents by using ELISA.Serum lactate dehydrogenase (LDH) and creatinkinase isoenzyme (CK-MB) le-vels were measured at T0,T3 and T4 by using an automatic biochemistry analyzer. The infarct size was detected by Evan's blue and tetrazolium chloride (TTC) staining. Myocardial TNF-α and histamine contents were detected by ELISA. The percentage of mast cell degranulation was determined by toluidine blue staining. Results Following MI/R,serum LDH and CK-MB levels at phase T3 and T4,serum TNF-α and histamine contents at phase T2 and T3 ,and myocardial mast cell degranulation rate increased significantly,and myocardial TNF-α and histamine contents decreased in model group in comparison with pre-MI/R (P<0.05). Compared with IR model group,serum LDH and CK-MB levels at phase T3 and T4 ,myocardial TNF-α and histamine contents all decreased significantly (P<0.05),but serum TNF-α and histamine contents increased significantly in EA group(P<0.05). The infarct size was remarkably smaller in EA group than that in IR model group(P<0.05). Conclusion "Neiguan" (PC 6)-EA preconditioning has a cardioprotective effect on the ischemia-reperfusion myocardium by promoting mast cell degranulation.  
      关键词:Electroacupuncture;Neiguan (PC 6);Preconditioning;Myocardial ischemia reperfusion;Cardiac mast cells   
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    • CUI Jing-jing, GAO Jun-hong1, FU Wei-xing1, WANG Yu-min, MA Shu-hua, ZHANG Meng2, CUI Hai-feng, YU Xiao-chun
      Vol. 35, Issue 3, Pages: 188-192(2010) DOI: 10.13702/j.1000-0607.2010.03.005
      摘要:Objective To investigate the functional specificity of acupoint by means of acupoint injection of Dobutamine Hydrochloride (DH). Methods Male SD rats were randomized into normal control (NC),EA-Neiguan (PC 6)+acupoint injection (AI,EA-PC 6+AI),EA-Zusanli (ST 36)+AI (EA-ST 36+AI),and EA-intramuscular injection site (IMIS)+intramuscular injection (EA-IMIS+IMI) groups,with 8 rats being in each group. EA (2 Hz/15 Hz,3 mA) was applied to bilateral "Neiguan" (PC 6),"Zusanli " (ST 36) and muscular-injection site for 10 min. DH (a β1-adrenoceptor agonist) solution (100 μg/kg) was injected intramuscularly into the gluteus maximus after termination of EA intervention. The left ventricular systolic pressure (LVSP),heart rate (HR) and +dp/dt max were recorded before and 2,5,15 and 30 min after injection of DH. Results The values of LVSP,HR and +dp/dt max in EA-PC 6+AI group were significantly higher than those in NC,EA-ST 36+AI,and EA-IMIS+IMI groups (P<0.01) 2 and 5 minutes after EA plus acupoint injection of DH. No significant differences were found between EA-ST 36+AI and EA-IMIS+IMI groups in LVSP,HR and +dp/dt max (P>0.05). After injection of DH for 30 minutes,the values of LVSP,HR and +dp/dt max in EA-PC 6+AI group were still significantly higher than those in NC group (P<0.01). Conclusion EA-PC 6+AI DH is significantly superior to EA-ST 36+AI DH and EA-IMIS+IMI DH in producing a stronger cardiac excitatory effect and a longer post-effect,showing a relative specificity of the acupoint in upregulating cardiac functional activities.  
      关键词:Neiguan (PC 6);Electroacupuncture;Acupoint injection;Functional specificity of acupoint;Cardiac effect   
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      更新时间:2023-08-11
    • CHENG Bin, SHI Hong1, JI Chang-fu1, LI Jiang-hui1, CHEN Shu-li1, JING Xiang-hong1
      Vol. 35, Issue 3, Pages: 193-197(2010) DOI: 10.13702/j.1000-0607.2010.03.006
      摘要:Objective To observe the dynamic distribution of the extravasated Evans Blue (EB) dye points (neurogenic inflammatory response) at the skin after acute gastric mucosal injury (AGMI) and its relation to the related regular acupoints in the locations in rats. Methods A total of 70 Wistar rats were randomized into normal control (n=10),normal saline (n=10),and AGMI (n=50) groups. The AGMI group was further divided into 5 h,2 d,3 d,4 d and 5 d subgroups with 10 rats in each. AGMI model was duplicated by intragastric perfusion of diluted hydrochloric acid (HCl,0.5 mol/L). Evans Blue Dye (50 mg/kg,50 mg/mL in 0.9% saline) was given to the rats before AGMI modeling. The plasma extravasated EB points at the skin of the whole body were observed after removal of the hair. Results The extravasated EB points presented a nerve-segmental distribution,with the proportion of the points in the location being 47.5% for "Geshu" (BL 17),58.82% for "Jizhong" (GV 6),88.23% for "Pishu" (BL 20),82.35% for "Weishu" (BL 21),17.64% for "Zhongwan" (CV 12),and 5.88% for "Shangwan" (CV 13),respectively. The plasma extravasation of EB seldom appeared in normal rats and only fewer points were found in rats accepted administration of 0.9% saline. Significant differences were found between model and normal control groups,and between model and normal saline groups in the numbers of the extravasated EB points (P<0.01,P<0.05). The number of the extravasated EB points was related to the phase of gastric mucosa injury,being most on the 2nd and 3rd day after modeling and disappearing gradually along with the natural repair of AGMI. Conclusion AGMI promotes the plasma extravasation of EB and the extravasated EB points present a nerve-segmental distribution and have a higher corresponding rate with some acupoints including "Pishu" (BL 20),"Weishu" (BL 21),etc.,suggesting an activation of the normally silent acupoints under diseased conditions.  
      关键词:Acute gastric mucosal injury;Evan's blue extravasation;Activation of acupoints;Neurogenic inflam-mation   
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    • GAO Jun, LIU Xu-guang, HUANG Di-jun, TANG Yong, ZHOU Hai-yan, YIN Hai-yan, CHEN Ting
      Vol. 35, Issue 3, Pages: 198-203(2010) DOI: 10.13702/j.1000-0607.2010.03.007
      摘要:Objective To observe the effect of moxibustion of "Shenshu" (BL 23) and "Zusanli" (ST 36) on synovial nuclear factor (NF)-kB p 65 expression,and plasma adrenocorticotropic hormone (ACTH) and serum cortisol (CS) contents in rheumatic arthritis (RA) rats with adrenalectomy (ADX) so as to study the underlying mechanism in ameliorating RA. Methods Fifty male SD rats were randomly divided into control (n=10),model (n=10),moxibustion (n=10),ADX (n=10) and false ADX (n=10) groups according to SPSS-aided random digits table. RA model was established by injecting 0.1 mL Freund's complete adjuvant into the right paw. ADX operation was performed on the sixth day after successful establishment of RA model. Moxibustion was given to "Shenshu" (BL 23) and "Zusanli" (ST 36) from the 7th day on,five moxa cones each session,and once daily for 18 days. Then plasma ACTH and serum CS contents,for which the blood was collected during night,were detected by ELISA,and NF-kB p 65 immunoactivity in synovial tissue was detected by immunohistochemistry. Results In comparison with control group,the foot volumes (swelling degree) of model,moxibustion,ADX,and false ADX groups increased significantly (P<0.01),while compared with model group,the swelling degrees of moxibustion and false ADX groups were decreased remarkably after the treatment (P<0.01). No significant difference was found between ADX and model groups in the foot volume (P>0.05). Compared with control group,plasma ACTH content in RA model group decreased obviously (P<0.05),while serum CS level and NF-kB p 65 immunoactivity increased apparently in model group (P<0.01). Compared with model group,serum CS contents in moxibustion,ADX and false ADX groups and synovial NF-kB p 65 immunoactivity in moxibustion and false ADX groups reduced considerably (P<0.01,P<0.05). No significant difference was found between ADX and model groups in synovial NF-kB p 65 immunoactivity (P>0.05). Conclusion Moxibustion treatment can reduce inflammation reactions in RA rats,which is closely associated with its effects in upregulating plasma ACTH,downregulaing serum CS level and synovial NF-kB p 65 immunoactivity,and the intact hypothalamus-pituitary-adrenal axis (HPAA).  
      关键词:Moxibustion;Rheumatoid arthritis;HPAA;Synovial NF-kB p 65   
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    • YU Jin-na, MA Xiao-jing, LIU Zhi-shun, WU Zhen-yu1, ZHANG Yun1, WU Guang-jun1, DENG Yan-hua, GU Bao-guang
      Vol. 35, Issue 3, Pages: 204-207(2010) DOI: 10.13702/j.1000-0607.2010.03.008
      摘要:Objective To observe the effect of electroacupuncture (EA) at "Ciliao" (BL 32) on detrusor hyperreflexia and c-fos expression in the sacral segment of spinal cord in rats with spinal cord injury (SCI). Methods Thirty-seven adult female Sprague-Dawley rats were randomized into normal control (n=5),SCI model (n=16) and EA (n=16) groups. EA(20 Hz,3 mA) was applied to bilateral BL 32 for 2 hours,once daily for 14 days. Intravesical pressure was detected by using a pressure transducer and a bioelectric amplifier. The expression of c-fos gene was detected by immunohistochemistry. Results In comparison with normal control group,the maximum intravesical pressure (MIVP)raised significantly in model group (P<0.05),and the compliance of the bladder decreased remarkably (P<0.05). While compared with model group,MIVP decreased significantly in SCI rats of EA group after EA intervention (P<0.05),and the vesical compliance increased obviously (P<0.05). In comparison with normal control group,the mean optical density (OD) value of c-fos immuno-reaction (IR) positive products increased significantly in the sacral cord after SCI in model group (P<0.05),while compared with model group,the mean OD value of c-fos IR positive products in EA group declined evidently but still being higher than that of normal control group (P<0.05),displaying a downregulation of c-fos expression after EA. Conclusion Electroacupuncture at "Ciliao" (BL 32) can inhibit the over-activity of bladder in SCI rats and reduce the c-fos expression in the sacral cord,suggesting that the declined C-fibers' activity after EA may be one of its mechanism underlying improving detrusor hyperreflexia in spinal cord injury.  
      关键词:Spinal cord injury;Detrusor hyperreflexia;Electroacupuncture;Ciliao (BL 32);c-fos expression   
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    • BU Yuan, ZHANG Pei-ying, GENG De-qin1, CAO Jin-xia1, LI Ji-zhen1
      Vol. 35, Issue 3, Pages: 208-212(2010) DOI: 10.13702/j.1000-0607.2010.03.009
      摘要:Objective To observe the effect of electroacupuncture (EA) plus oxygenmedicine (OM) on the expression of Bcl-2 and Bax in the hippocampal CA 1 area in cerebral ischemia/reperfusion injury (CI/RI) rats. Methods Thirty SD rats were randomized into sham-operation,model,EA,OM,EA+OM groups (n=6/group). CI/RI model was established by using modified Pulsinelli 4 vessel occlusion and reperfusion. EA (100 Hz,3.5 mA) was applied to "Baihui" (GV 20) and "Zusanli" (ST 36) 30 min,once daily for 4 days. Rats of OM and EA+OM groups were put into a box filled with oxygen and atomized herbal medicines containing Bingpian (Borneolum),Shexiang (Moschus),Huangjing (Rhizoma Polygonati),Shouwu (Radix Polygoni Multiflori),etc. for 30 min,once daily for 4 days. Bcl-2 and Bax expression of the hippocampal CA 1 area was detected by immunohistochemistry. Results Compared with sham group,the numbers of Bcl-2 immunoreaction (IR) and Bax IR positive cells,and the immunoactivity of Bcl-2 IR and Bax IR positive products in the hippocampal CA 1 area were increased significantly in model group (P<0.05,P<0.01). In comparison with model group,the number of Bcl-2 IR positive cells and Bcl-2 immunoactivity in EA,OM and EA+OM groups increased considerably (P<0.01),while Bax IR positive cell numbers and Bax immunoactivity in EA,OM and OM+EA groups decreased significantly (P<0.01). The effects of EA+OM were significantly superior to those of EA and OM groups in upregulating Bcl-2 IR positive cell number and Bcl-2 immunoactivity and downregulating Bax IR positive cell number and Bax immunoactivity (P<0.01). No significant differences were found between EA and OM groups in the abovementioned indexes (P>0.05). Conclusion EA and OM and EA+OM can effectively regulate the expression of Bcl-2 and Bax in the hippocampal CA 1 area in CI/RI rats,and the effects of EA+OM are significantly superior to those of simple EA and simple OM,which may contribute to their effect in improving cerebral ischemia.  
      关键词:Cerebral ischemia/reperfusion;Electroacupuncture;Oxygenmedicine;Hippocampal CA 1 area;Bcl-2;Bax   
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    • CHEN Ming, HU Xiang-long, WU Zu-xing
      Vol. 35, Issue 3, Pages: 213-216(2010) DOI: 10.13702/j.1000-0607.2010.03.010
      摘要:Objective To observe changes of the partial oxygen pressure in the deep tissues along the Large Intestine Meridian (LIM) during acupuncture stimulation,so as to reveal the characteristics of energy metabolism in the tissues along the LIM. Methods Thirty-one healthy volunteer subjects were enlisted in the present study. Partial oxygen pressure (POP) in the tissues (at a depth of about 1.5 cm) of acupoints Binao (LI 14),Shouwuli (LI 13),Shousanli (LI 10),2 non-acupoints [the midpoints between Quchi (LI 11) and LI 14,and between Yangxi (LI 5) and LI 11) of the LIM,and 10 non-meridian points,1.5-2.0 cm lateral and medial to each of the tested points of the LIM was detected before,during and after electroacupuncture (EA) stimulation of Hegu (LI 4) by using a tissue oxygen tension needle-like sensor. Results In normal condition,the POP values in the deep tissues along the LIM were significantly higher than those of the non-meridian control points on its bilateral sides. During and after EA of Hegu (LI 4),the POP levels decreased significantly in the deep tissues along the LIM in comparison with pre-EA (P<0.01),and had no apparent changes in the non-meridian control points (P>0.05). Conclusion POP is significantly higher in the deep tissues along the LIM of healthy subjects under normal conditions,which can be downregulated by EA of Hegu (LI 4),suggesting an increase of both the utilization rate of oxygen and energy metabolism after EA.  
      关键词:Large Intestine Meridian;Oxygen partial pressure;Energy metabolism;Electroacupuncture   
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    • MA Wen-zhu, ZHOU Pei-juan, ZHANG Yi, YUAN Ying, WU Ying1, ZHAO Chun-hua2, XIA Ying-li3
      Vol. 35, Issue 3, Pages: 217-221(2010) DOI: 10.13702/j.1000-0607.2010.03.011
      摘要:Objective To observe the effect of electroacupuncture (EA) of Sanyinjiao (SP 6) on labor in parturients. Methods A total of 349 parturients were randomly divided into EA (n=117),sham-EA (n=117) and control (n=115) groups. The visual analog scale (VAS) was used to assess the pain intensity of puerperas with labor before and after acupuncture intervention. After excluding those puerperas with cesarean section,the time required for each stage of labor and the total duration of labor were observed in 286 cases of natural delivery women including 92 cases in the control group,94 cases in the sham-EA group and 100 cases in the EA group. EA (2 Hz/100 Hz,20 mA) was given to the puerperas for 30 min after the needle in a cannula was tapped into Sanyinjiao(SP 6)and when the dilatation of cervix was about 2-3 cm wide. For puerperas of sham-EA group,the operation was similar,but no real acupuncture needle and no real electric current were given. Results The VAS score for childbirth pain intensity was significantly lower in EA group than in control group(P<0.05)and had no significant difference between sham-EA and control group (P>0.05). The duration of the active phase of the first stage of labor was significantly lower in EA group than in sham-EA group (P<0.05),but had no significant difference between sham-EA and control groups (P>0.05). No significant differences were found among the 3 groups in the latency of the first stage of labor,in the duration of the 2nd and the 3rd stages of labor and in the total phase of labor (P>0.05). Conclusion EA of SP 6 can relieve the pain intensity of the labor and shorten the duration of the active period of first stage of labor in puerperas,suggesting an improvement of the quality of deli-very after EA.  
      关键词:Puerpera;Labor;Electroacupuncture;Sanyinjiao(SP 6)   
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    • YAN Xing-ke, ZHANG Yan, YU Lu, YANG Bo, CHEN Cheng, WANG Fu-chun
      Vol. 35, Issue 3, Pages: 222-225(2010) DOI: 10.13702/j.1000-0607.2010.03.012
      摘要:Objective To observe the effect of "Tranquilization Needling" (TN) and acupuncture of "Eight Confluence Acupoints" (ECA) on Pittsburgh Sleep Quality Index (PSQI) in patients with insomnia of the deficiency type of both heart and spleen. Methods A total of 60 insomnia patients with the deficiency of both heart and spleen were randomly divided into TN group and ECA group. For patients of TN group,Sishencong (EX-HN 1),Shenmen (HT 7) and Sanyinjiao (SP 6) were punctured,and for those of ECA group,Shenmai (BL 62) and Zhaohai (KI 6) were punctured,once daily for 40 sessions. The PSQI score was used to assess the changes of the patients' sleeping quality. Results After the treatment,of the two 30 cases of TN group and ECA group,12 (40.0%) and 7 (23.3%) were cured,11 (36.7%) and 10 (33.3%) experienced marked improvement,5 (16.6%) and 8 (26.7%) had an improvement,and 2 (6.7%) and 5 (16.7%) failed,with the total effective rates being 93.3% and 83.3% respectively. The effective rate of TN group was significantly higher than that of ECA group (P<0.05). Self-comparison showed that PSQI scores of the sleeping quality,ramp duration,sleep efficiency and the total PSQI score in these two groups all declined significantly after the treatment (P<0.05,P<0.01),and the scores of ramp duration and the total PSQI score of TN group were evidently lower than those of ECA group (P<0.05). After the treatment,the integrative scores of symptoms of two groups both decreased significantly (P<0.01),and the integrative score of TN group was remarkably lower than that of ECA group (P<0.05). Conclusion Both TN and acupuncture of ECA can effectively improve insomnia patients' clinical symptoms,and the therapeutic effect of TN is more effective than that of ECA in shortening ramp duration and the total PSQI score.  
      关键词:Insomnia;Deficiency of both heart and spleen;Tranquilization needling;Eight Confluence Acupoints;Pittsburgh Sleep Quality Index   
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    • LI Su-yun, ZHANG Li-jian, GANG Wei-juan, XU Wen-bin, XU Qing-yan
      Vol. 35, Issue 3, Pages: 226-228(2010) DOI: 10.13702/j.1000-0607.2010.03.013
      摘要:On the basis of systematicly reviewing the developmental history of acupuncture and moxibustion and profoundly understanding its academic connotations,the authors of the present article make a summary and analysis on the key factors influencing the development of acupuncturology. These key factors are 1) the emergence of "microacupuncture needle regulating-Qi" and the establishement of their corresponding theory system,2) a large number of practitioners who inherited the learnings of acupuncturology generations by generations,and abundant medical classical works which recorded the valuable academic thoughts and clinical experience of the predecesors,3) the application of acupuncture charts and manikins,and 4) modernizing changes of acupuncture learnings after introduction of western medicine to China. Just under the influence of these key factors,the acupuncture medicine separates itself from the level of the simple experience medicine,and has formed a set of special theory system and developed into a mature subject.  
      关键词:Acupuncturology;Medical history;Inheritance   
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    • BEN Hui, RONG Pei-jing, GAO Xin-yan, LI Liang, HE Wei
      Vol. 35, Issue 3, Pages: 229-231(2010) DOI: 10.13702/j.1000-0607.2010.03.014
      摘要:Ziwu-liuzhu (Midnight-noon Ebb-flow) acupuncture therapy is a natural therapy by taking the time as a principal condition. It has been being widely applied in clinical practice. In the present paper,the authors review the progress of clinical application and related researches on Midnight-noon Ebb-flow acupuncture method in the past years,and also summarize some scholars' opinions about its extant problems and shortcomings.  
      关键词:Ziwu-liuzhu acupuncture therapy;Clinical application;Experimental studies   
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    • Review on the Safety of Deep Acupuncture at Tianshu(ST 25)

      DUAN Jin-xiu, LIU Zhi-shun
      Vol. 35, Issue 3, Pages: 232-235(2010) DOI: 10.13702/j.1000-0607.2010.03.015
      摘要:Tianshu (ST 25) is a frequently-used acupoint in acupuncture practice. It is reported that deep acupuncture has a better therapeutic effect than routine depth acupuncture. On the basis of reviewing the available literature,the present paper gives a definition about "deep acupuncture" of Tianshu (ST 25) from the anatomy angle,and makes a summary about its safety. This paper also puts forward a set of operation standards for needling Tianshu (ST 25). Moreover,the authors of the present paper also make a systematic analysis on the factors affecting the safety from the punctured depth,the diameter of the acupuncture needle and the operation technique. These methods and standards have an important significance in guiding clinical practice of acu-puncture.  
      关键词:Tianshu;Deep acupuncture;Safety   
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    • Comments on the Thickness of Abdominal Acupuncture Needles

      WANG Li-ping, ZHANG Bei
      Vol. 35, Issue 3, Pages: 236-240(2010) DOI: 10.13702/j.1000-0607.2010.03.016
      摘要:In the present paper,the authors make a summary about the development of acupuncture needles in Chinese ancient times recorded in the literature,and have a discussion on the effect of the thickness of acupuncture needles on clinical the-rapeutic efficacy in accordance with modern medical theories,the development of its clinical application and by combining their own experience in acupuncture practice. They conclude that the thickness of the abdominal needle is a negligible factor influencing the therapeutic efficacy of the reinforcing-reducing manipulations. In the treatment of deficiency pattern disorders,a thinner acupuncture needle is suitable,while for excess syndrome,a thicker acupuncture needle is recommended. In the light of the patient's constitution and different state of clinical conditions,a flexible application of the acupuncture needle with different diameters is highly recommended in clinical practice. In this way,the clinical therapeutic effect would be improved much.  
      关键词:Abdominal acupuncture;Acupuncture needle;Thickness of needle;Clinical efficacy   
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