最新刊期
卷 36 ， 期 4 ， 2011
- 摘要：Objective To observe the effect of moxibustion on colonic tumor necrosis factor(TNF)-α level in Crohn's Disease(CD) rats and the effect of colonic supernatant of CD rats experiencing moxibustion on the expression of the tight junction proteins occludin,claudin-1 and zonula occludens(ZO)-1 and their genes in the cultivated colonic epithelial cells derived from CD rats,so as to reveal its underlying mechanism in resisting colonic epithelial barrier defects.Methods Sixty SD rats were randomized into normal control,model,moderately warm moxibustion(MWM),herbs-partitioned moxibustion(HPM) and medication(salazosulfapyridine,SASP) groups(n=12).CD model was established by intra-annual perfusion of trinitrobenzene sulfonic acid(TNBS) solution(TNBS∶50% alcohol= 2∶1,0.5 mL/kg).For rats of the HPM and MWM groups,moxibustion was given to "Tianshu"(ST 25) and "Qihai"(CV 6) once daily for 14 d.For rats of the medication group,intragastric perfusion of SASP solution(0.0405 g/3 mL) was given twice daily for 14 d.After the treatment,all the rats including those of normal group were killed for preparing the supernatant of colonic mucosa tissue(6-8 cm superior to the anus).The colonic epithelial cells of the normal group were purified and cultivated in DMEM culture fluid containing the prepared supernatant of normal group to establish an intestinal epi-thelial barrier defect model,and also cultured separately in the media containing the prepared supernatants of the model,medication,HPM and MWM groups.One week after the culture,the expression levels of occludin,claudin-1 and ZO-1 proteins and their genes in the cultured colonic epithelial cells were detected by Western blot and fluorescent quantitative polymerase chain reaction assay respectively.TNF-α content of the colonic supernatant was detected by enzyme linked immunosorbent assay.Results Compared with the normal group,colonic TNF-α content was remarkably increased in the model group(P<0.01).In comparison with the model group,colonic TNF-α contents were significantly decreased in the medication,MWM and HPM groups(P<0.01),and those of the MWM and HPM groups were markedly lower than that of the medication group(P<0.05).The expression levels of the cultured normal colonic epithelial occludin,claudin-1 and ZO-1 proteins and their mRNAs in the medication,MWM and HPM groups were remarkably increased compared with those in the model group(P<0.01,P<0.05).The expression levels of colo-nic epithelial occludin,claudin-1 and ZO-1 proteins and their mRNAs were significantly higher in the MWM and HPM groups than in the medication group(P<0.05).Conclusion Both MWM and HPM can downregulate colonic mucosal TNF-α content in CD rats,and the colonic supernatant of rats undergoing MWM and HPM may upregulate the expression of colonic epithelial occludin,claudin-1 and ZO-1 proteins and their mRNAs in the cultivated colonic epithelial cells,which may contribute to the effect of moxibustion in relieving colonic epithelial barrier defect.关键词：Moxibustion;TNF-α;Colonic epithelial cells;Tight junction proteins and genes;Occludin;Claudin-1;ZO-11177|799|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of scalp-acupuncture on N-acetyl-aspartate(NAA) and choline(Cho) leve-ls in the brain tissue in rabbits with intracerebral hemorrhage(ICH) so as to explore its underlying mechanisms in resisting neural lesion.Methods A total of 30 Newzealand rabbits were randomly divided into normal,model and acupuncture groups(n=10).ICH model was duplicated by injection of the rabbit-own blood(200 μL) into the bilateral Basal Nuclei(A: 2 mm;R,L: 5 mm;H: 10 mm).A filiform needle was inserted into "Baihui"(GV 20) to "Taiyang"(EX-HN 5) on the hemorrhage side,manipulated for 1 min,and then retained for 30 min(manipulated once again every 10 min).The treatment was conducted once daily for 7 days.Contents of NAA,Cho and creatine(Cr) in the intracerebral peri-hematoma tissues were detected by using 1H-Magnetic Resonance Spectroscopy(MRS).Results Compared with the normal group,NAA/Cr ratios at the time points of 2 h,3 d and 7 d after ICH in the model group were decreased markedly(P<0.05),while Cho/Cr ratios at the same 3 time-points after ICH in the model group were increased markedly(P<0.05).In comparison with the model group,NAA/Cr ratio on day 3 after ICH in the acupuncture group was obviously higher(P<0.05),while Cho/Cr ratios on day 3 and 7 were significantly down-regulated in the acupuncture group(P<0.05).No significant changes were found in NAA/Cr ratios at 2 h and on day 7 and in Cho/Cr ratio at 2 h after ICH between the acupuncture group and the model group.Conclusion Penetrative acupuncture of GV 20-EX-HN 5 is effective in suppressing ICH-induced increase of Cho/Cr ratio in ICH rabbits,which may contribute to its function in accelerating neural repair process.关键词：Scalp-acupuncture;Intracerebral hemorrhage;Neural lesion;1H-MRS1017|218|0更新时间：2023-08-11
- 摘要：Objective To observe the alternations of local mast cells(MCs) in the number and functional activities after electroacupuncture(EA) and injection of stem cell factor(SCF) antibody into "Weishu"(BL 21) and "Zusanli"(ST 36) in rats.Methods Thirty Wistar rats were randomly assigned to normal,EA,and EA+SCF antibody injection(EA-ABI) groups(n=10).EA was applied to the left "Weishu"(BL 21) and "Zusanli"(ST 36) for 25 min.For rats of EA+ABI group,SCF-AB(1∶200,0.1 mL) was respectively injected into BL 21 and ST 36,following by EA.The tissues around the two acupoints areas were sampled to be stained with acetylcholine esterase(AchE) and toluidine blue(TB),respectively.The counts and degranulation ratio of MCs in the acupoint region were recorded and observed under microscope.Results In comparison with the normal group,MCs count was increased significantly in the EA of "Weishu"(BL 21) group(P<0.05),mildly in the EA of "Zusanli"(ST 36) group(P>0.05),and downregulated considerably in both EA-ABI-BL 21 and EA-ABI-ST 36 groups(P<0.05).Compared with the normal group,the degranulation ratio of MCs was increased significantly in EA-BL 21,EA-ST 36,EA-ABI-BL 21 and EA-ABI-ST 36 groups(P<0.05).No significant differences were found between EA-BL 21 and EA-ABI-BL 21 groups,and between EA-ST 36 and EA-ABI-ST 36 groups in the degranulation ratios(P>0.05).Conclusion Local injection of SCF antibody in BL 21 and ST 36 can significantly suppress EA-induced increase of the number and degranulation ratio of local subcutaneous MCs in rats,suggesting that SCF is an important factor affecting the activity and recruitment of MCs around the acupoint during EA.关键词：Stem cell factor;Subcutaneous mast cells;Electroacupuncture;Acupoint injection;Weishu(BL 21);Zusanli(ST 36)1397|283|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) intervention on the expression of norepinephrine transporter(NET) mRNA in the middle cervical-stellate ganglion complex(MC-SGC) and β1-adrenergic receptor(β1-AR) mRNA in the left ventricular myocardium in cerebral-cardiac syndrome(CCS) rats,so as to study its underlying mechanism in the prevention and treatment of CCS.Methods Eighteen Wistar rats were randomized into sham operation,model and EA groups(n=6).CCS model was duplicated by intravenous injection of 1 μL of saline containing collagenase(1 U/μL) +heparin(7 U/μL) into the caudate nucleus.EA(2 Hz,5 V) was applied to "Shuigou"(GV 26)-"Fengfu"(GV 16),and "Neiguan"(PC 6)-"Xinshu"(BL 15) for 20 min,once daily for 3 days.Seventy-two hours after modeling,pathological changes of the brain and cardiac tissues were observed after sectioning and stained with HE method.The expression of MC-SGC NET mRNA and the myocardial β1-AR mRNA of the left ventricle were detected by fluorescence quantitative PCR.Results ①In comparison with the model group,the number of the exuded red blood cells in the brain tissue around the caudate Nucleus region was fewer,the severity of the tissue edema,cell necrosis,and inflammatory cell infiltration lighter in the EA group.Compared with the model group,the severity of degeneration and necrosis of myocytes was lighter and the number of the broken cardiac smooth muscle fewer in the EA group.② The expression levels of both MC-SGC NET mRNA and myocardial β1-AR mRNA were significantly lower in the model group than in the sham operation group(P<0.01).Compared with the model group,the expression levels of both MC-SGC NET mRNA and myo-cardial β1-AR mRNA were upregulated considerably in the EA group(P<0.01).Conclusion EA of GV 26-GV 16 and PC 6-BL 15 can upregulate the expression of both MC-SGC NET mRNA and myocardial β1-AR mRNA in CCS rats which may contribute to its effect in the prevention and treatment of CCS syndrome.关键词：Cerebrocardiac syndrome;Electroacupuncture;Norepinephrine transporter mRNA;Middle cervical-stellate ganglion complex;Myocardial β1 adrenergic receptor mRNA1205|408|3更新时间：2023-08-11
- 摘要：Objective To study the mechanism of moxibustion in postponing the process of aging.Methods Thirty Wistar rats were equally randomized into control,model and moxibustion groups.The subacute aging model was established by hypodermic injection of 25% D-galactose(125 mg/kg).Moxibustion was applied to bilateral "Shenshu"(GV 23) and "Pishu"(GV 20) once everyday for 6 weeks.After slicing,the pineal gland tissue was stained with HE and insitu hybridization methods respectively for detecting the quantity of pinealocytes and the expression of heat shock protein(HSP 70).Results Compared with the control group,both the quantity of pinealocytes and the expression of HSP 70 in the pineal gland in the model group were downregulated significantly(P<0.001,P<0.01).Compared with model group,the quantity of pinealocytes and of HSP 70 mRNA in the pineal gland of moxibustion group were upregulated significantly(P<0.01,P<0.05).Conclusion Moxibustion can suppress aging induced decrease of pinealocyte number and HSP 70 expression in subacute aging rats,which may contribute to its effect in postponing aging.关键词：Moxibustion;Aging;Pineal gland;Pinealocytes;Heat shock protein 70 expression1393|255|1更新时间：2023-08-11
- 摘要：Objective To study the specific correlation between "Jinggu"(BL 64) and "Dazhong"(KI 4) in the nervous system by using a double-labeling of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594(CTB-Alexa 488,594) in rats,so as to investigate its neuroanatomical basis for clinical joint-application of Yuan-Source and Luo acupoints.Methods Three male SD rats were used in the present study.Under anesthesia(10% urethane),0.1% CTB-Alexa 488(5 μL) and CTB-Alexa 594(5 μL) were respectively injected into the border area between the red and white flesh,distal to the tuberosity of the fifth metatarsal bone,and the depression anterior to the medial attachment of the calcaneal tendon,the corresponding sites of the acupoints Jinggu(BL 64) and Dazhong(KI 4) in the human body.After 3 surviving days,the rat's brain,spinal cord and dorsal root ganglia(DRGs) of L3-L6 were dissected following perfusion with 4% paraformaldehyde,cut into sections and observed under fluorescent microscope equipped with a digital camera.The labeled neurons were recorded and counted.Results It was found under fluorescent microscope that the single-labeled neurons and the dual-labeled neurons were ipsilaterally located on the injected side.Among the single-labeled neurons,the labeled sensory neurons related to "Jinggu"(BL 64) and "Dazhong"(KI 4) were found to be in the DRGs of L3-L6,with a higher concentration in the DRGs of L4(27/162,102/332) and L5(130/162,204/332).The dual-labeled 7 neurons were found to be in DRGs of L4 and L5.In addition,the labeled motoneurons related to "Jinggu"(BL 64) and "Dazhong"(KI 4) distributed in the dorsolateral portion of lamina Ⅸ,forming a longitudianal column from L3-L6 with a higher concentration at L4 and L5.Conclusion The labeled sensory and motor neurons innervating Yuan-acupoint "Jinggu"(BL 64) and Luo-acupoint "Dazhong"(KI 4) distribute in DRGs of the same spinal segments and spinal ventral horns from L3-L6.关键词：Jinggu(BL 64);Dazhong(KI 4);Innervation;Fluorescent double-tracing;Sensory neurons;Motor neurons1189|610|11更新时间：2023-08-11
- 摘要：Objective To study the underlying mechanism of acupuncture of "Zusanli"(ST 36) in clinical treatment of spleen deficiency syndrome(SDS).Methods Twenty-six adult male Wistar rats were randomized into normal control group(n=6),model group(n=8),saline+model(saline) group(n=6) and acupuncture+model(acupuncture) group(n=6).The spleen deficiency syndrome model was duplicated by intragastric perfusion of 50% alcohol(1 mL/100 g,once) and vinegar(pH 3,1 mL/100 g,once daily for 10 d).Bilateral "Zusanli"(ST 36) was punctured with filiform needles which were manipulated for 1 min after insertion and retained for 20 min.The treatment was conducted once daily for 10 days.The contents of serum testosterone(T) and estradiol(E2)were detected by radioimmunoassay.The rats' physical strength was detected by exhausted swimming test in cool water.Results In comparison with the normal group,the duration of exhausted swimming,serum T and E2 contents and T/E2 were decreased significantly in the model group(P<0.05).Compared with the model group,the duration of exhausted swimming,serum T and E2 contents and T/E2 in the acupuncture group were upregulated significantly(P<0.05).No significant differences were found between the saline and control groups(P>0.05).Conclusion Acupuncture of "Zusanli"(ST 36) is able to reverse SDS-induced decrease of sexual hormones in spleen deficiency syndrome rats,which may contribute to its effect in improving physical strength.关键词：Spleen deficiency syndrome;Acupuncture;Zusanli(ST 36);Testosterone;Estradiol1377|312|5更新时间：2023-08-11
- 摘要：Objective To observe the effects of electroacupuncture(EA) of "Zusanli"(ST 36) on serum TNF-α and IL-6 contents and pancreatic nuclear factor kappa-B(NF-κB) expression in acute pancreatitis rats.Methods Sixty-six male SD rats were randomly divided into sham operation(sham),model and EA groups(n=22).Acute pancreatitis model was established by intra-pancreatic duct injection of 3.5% sodium taurocholate(0.1 mL/100 g).EA(2 Hz /100 Hz,2 mA) was applied to bilateral ST 36 for 30 min after modeling and at the end of the experiment.The animals were killed at 3 h(n=7),6 h(n=7) and 12 h(n=8) after modeling.The expression of pancreatic NF-κB P 65 was detected by immunohistochemical staining,and serum TNF-α and IL-6 contents were determined by ELISA.The pathological changes of pancreatic tissue were displayed by H.E.staining and the quantity of ascite was measured by electronic balance.Results The pathologic score,ascite quantity,serum TNF-α and IL-6 contents,and pancreatic NF-κB P 65 expression levels at 3 h,6 h and 12 h after modeling were significantly higher in the model group than in the sham group(P<0.05).Compared with the model group,the pathologic score,ascite quantity,se-rum TNF-α and IL-6 contents,and pancreatic NF-κB P 65 expression levels at 3 h,6 h and 12 h were significantly decreased in the EA group(P<0.05).Microscopic observation displayed that the necrosis of the pancreatic acinar cells and infiltration of inflammatory cells were lighter in the EA group than in the model group.Conclusion EA at ST 36 is able to down-regulate sodium taurocholate injection induced increase of serum TNF-α and IL-6 contents,and pancreatic NF-κB P 65 expression level in acute pancreatitis rats,which may contribute to its effect in relieving necrosis of the pancreatic acinar cells and infiltration of pancreatic inflammatory cells.关键词：Acute pancreatitis;Electroacupuncture;Pancreatic NF-κB;TNF-α;IL-61456|257|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture of Zusanli(ST 36) on electroencephalogram(EEG) so as to probe into its law in regulating the interconnectivity of brain functional network.Methods A total of 9 healthy young volunteer students(6 male,3 female) participated in the present study.They were asked to take a dorsal position on a test-bed.EEG signals were acquired from 22 surface scalp electrodes(Fp1,Fp2,F7,F3,Fz,F4,F8,A1,T3,C3,Cz,C4,T4,A2,T5,P3,Pz,P4,T6,Oz,O1 and O2) fixed on the subject's head.Acupuncture stimulation was applied to the right Zusanli(ST 36) by manipulating the filiform needle with uniform reducing-reinforcing method and at a frequency of about 50 cycles/min for 2 min.Then the stimulation was stopped for 10 min,and repeated once again(needle-twirling frequency:150 and 200 cycles/min),3 times altogether.The acquired EEG data were analyzed by using coherence estimation method,average path length,average clustering coe-fficient,and the average degree of the articulation points(nodes) for analyzing the synchronization of EEG signals before,during and after acupuncture.Results In comparison with pre-acupuncture,the coherence amplitude values of EEG-δ(1-4 Hz) and γ(31-47 Hz) waves were increased significantly after acupuncture of ST 36.No significant changes were found in the amplitude values of EEG-θ(5-8 Hz),-α(9-13 Hz) and-β(14-30 Hz) waves after acupuncture stimulation.During and after acupuncture,the synchronism values of EEG-δ waves of different leads and numbers of interconnectivity between every two brain functional regions in majority of the 9 volunteers were increased clearly.In all volunteers,the degree values of all nodes except A 1 and A 2,the average clustering coefficients along with the increase of the threshold(r),and the average path lengths of the brain functio-nal network of EEG-δ waves during and after acupuncture were also increased evidently(the latter two items,P<0.05),suggesting an increase of the information exchange and functional connectivity of different brain regions.Conclusion Acupuncture of Zusanli(ST 36) can increase the amplitude and synchronization of EEG-δ waves of different leads,and potentiate the functional interconnectivity of brain functional network.关键词：Brain functional network;Acupuncture;Coherence estimation;Brain functional synchronization;Clustering coefficient;Average path length891|437|5更新时间：2023-08-11
- 摘要：Objective To study the relationship between serum leptin and insulin resistance,and to analyze the effect of acupuncture on serum leptin level in patients with type-Ⅱ diabetes mellitus(DM).Methods A total of 80 type-Ⅱ DM patients were randomized into acupuncture and medication groups.Acupuncture was applied to Yishu(EX),Feishu(BL13),Pishu(BL 20),etc.according to syndrome identification.The treatment was given once every other day for 12 weeks.For patients in the medication group,Glibenclamide(2.5-7.5 mg/time,1-2 times/d according to blood sugar level) was given for 12 weeks.Fasting blood glucose(FBG),fasting insulin(FINS) and fasting leptin(FLP) were detected by using glucose oxidase method,radioimmunoassay and ELISA,respectively.Insulin sensitivity index(ISI) and homeostasis model assessment-insulin resistance(HOMA-IR) were calculated.Results In comparison with pre-treatment,FBG levels and HOMA-IR in both acupuncture and medication groups,and FINS and FLP levels in the acupuncture group were decreased significantly(P<0.01),while ISI in both acupuncture and medication groups,and FINS level in the medication group were increased remarkably after the treatment(P<0.01).Comparison between two groups showed that after the treatment,FINS and FLP levels,and HOMA-IR of the acupuncture group were considerably lower than those of the medication group(P<0.01),while ISI of the acupuncture group was significantly higher than that of the medication group(P<0.01).Conclusion Acupuncture therapy is effective in lowering FLP level,which may contribute to its clinical effect in improving type-Ⅱ DM.关键词：Acupuncture therapy;Type-Ⅱ diabetes mellitus;Serum leptin;Insulin resistance1013|311|4更新时间：2023-08-11
- 摘要：Objective To assess the preemptive analgesia effect of catgut implantation in Chengshan(BL 57) area on hemorrhoid operation-induced pain and the security,so as to find a new method for easing post-operative pain of hemorrhoid.Methods A total of 120 cases of mixed hemorrhoids patients were randomly divided into the catgut implantation group and me-dication group(n=60).In implantation group,a piece of catgut was embedded into bilateral BL 57 acupoint area half an hour before surgery.Patients of the medication group were asked to orally take Tramadol Hydrochloride Capsules(100 mg) after the surgery.The pain score of mixed hemorrhoids patients was evaluated 5,24,48,72 and 96 h after operation by using visual analogue scales(VAS).The complications,analgesics use and adverse reactions were recorded simultaneously.Results Comparison between two groups showed that the VAS scores of the catgut implantation group at 5,24,48,72 and 96 h after operation were significantly lower than those of the medication group(P<0.05).The incidence of complications of uroschesis and hydroncus,adverse reactions of nausea,vomiting and vertigo,and the number of patients using Sauteralgyl were significantly lower in the catgut implantation group than in the medication group(P<0.05).Conclusion Catgut implantation at BL 57 can effectively relieve the postoperative pain,reduce postoperative adverse reactions and complications in mixed hemorrhoids patients.关键词：Catgut implantation;Mixed hemorrhoids;Postoperative pain and complications1296|457|4更新时间：2023-08-11
- 摘要：Metabonomics is a newly emerging modern technology in the Post-genome era and has been being used widely in the study on modern Chinese medicine.But it have been rarely used in the study on acupuncture therapy.In the present paper,the authors introduce the unique advantages of metabonomics in the systemtic biology and its widespread studying basis in the field of modern Chinese medicine.Metabonomics and Chinese medicine including acupuncturology are of some similar characteristics such as entirety,comprehensiveness and dynamic changes.Moreover,the authors make a primary discussion and strategic analysis on the key issues of researches about sham acupuncture,chrono-acupuncture,mechanisms of acu-moxibustion underlying improvement of clinical severe disorders and various indications problems,regularities of combination of acupoints in different recipes,etc.by using metabonomics.关键词：Metabonomics;Acupuncture;Research strategies2450|1251|6更新时间：2023-08-11
- 摘要：In the present paper,the authors review the development of experimental and clinical studies on acupuncture treatment of bronchial asthma in recent 10 years.Regarding clinical studies,results showed that acupuncture could 1) regulate cardiac-pulmonary function;and 2) adjust immune state and relieve inflammatory reactions in bronchial asthma patients.Animal experiments showed that acupuncture could function in 1) improving pulmonary function;2) reducing accumulation of the peripheral eosinophile granulocytes(EOS),relieving the infiltration of inflammatory cells in the air-passage mucosa and promoting the apoptosis of EOS in the lung and air-passages;3) down-regulating the expression of air-passage remodeling-related protein insulin growth factor-1;4) suppressing the secretion of tumor necrosis factor and endothelin;5) attenuating allergic reaction;6) regulating neuroendocrine activity;and 7) modulating intracellular second messenger activities.However,rigorous clinical study design is not enough,so that the reliability of the results is limited.In spite of many indicators of animal experiments have been selected,but their correlations are not in close association,resulting in poor complementation and mutual identification of the acquired findi-ngs.For this reason,its clinical efficacies need to be researched further according to principles of evidence-based medicine.关键词：Acupuncture therapy;Bronchial asthma;Clinical studies;Experimental studies993|630|8更新时间：2023-08-11
- 摘要：Our previous studies have showed that analogue electroacupuncture(EA) could induce a transmission of electrical signals along meridians,and excitatory neurotransmitters such as substance P and glutamate were involved in the transmission process.In recent studies,our results showed that somatostatin and its receptors played a dose-dependent tonic inhibition on the discharge rates of original and adjacent nerves innervating the acupoints.In addition,there existed an interaction between somatostatin and glutamate.These results suggest that the balance between excitatory and inhibitory neurotransmitters plays an important role in the function activities of acupoints and meridians.关键词：Meridian and collocterals;Bladder Meridian of Foot-Taiyang;Somatostatin;Electrical signal transmission along meridian1024|110|0更新时间：2023-08-11
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