最新刊期
卷 38 ， 期 2 ， 2013
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the expression of myocardial 1-phosphatidylinositol 3-kinase(PI 3 K),hypoxia-inducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF) in rats with ce-rebral-cardiac syndrome(CCS),so as to reveal its mechanism underlying reducing ischemic myocardial injury.Methods Forty SD rats were randomly and equally divided into sham-operation,model,EA and non-acupoint(the lateral-superior side of the hip) groups(10 rats/group).CCS model was established by injection of collagenase(1 U/μL) and heparin(7 U/μL) into the right caudate nucleus.Following modeling,EA(1.5 mA,2 Hz,20 min) was applied to "Shuigou"(GV 26),"Fengfu"(GV 16),"Neiguan"(PC 6) and "Xinshu"(BL 15) acupoints,once daily for three consecutive days.The expression levels of PI 3 K,HIF-1α and VEGF in the myocardium were detected by immunohistochemistry.Results Compared with the sham-operation group,the expression levels of myocardial PI 3 K,HIF-1α and VEGF proteins were significantly increased in the model group(P<0.01).While in comparison with the model group,there were little increase in the non-acupoint group(P>0.05) and considerable increase in the expression levels of the 3 myocardial proteins in the EA group(P<0.05).Conclusion EA intervention has a function in upregulating the expression of myocardial VEGF,HIF-1α and PI 3 K proteins in CCS rats,which maybe contribute to its protective effect on ischaemic myocardial injury.关键词：Electroacupuncture;Cerebral-cardiac syndrome;Myocardium;1-phosphatidylinositol 3-kinase;Hypoxia-inducible factor-1α;Vascular endothelial growth factor;Angiogenesis1153|348|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) at "Zusanli"(ST 36)-"Yanglingquan"(GB 34) on pain behavior and expression of hippocampal neuronal nitric oxide synthase(nNOS),inducible nitric oxide synthase(iNOS)and cGMP-dependent protein kinase(PKG) mRNA in rats with chronic neuropathic pain so as to analyze its mechanism underlying analgesia.Methods Forty male Wistar rats were randomly divided into control,CCI model,EA-2 Hz,EA-2 Hz/15 Hz,EA-100 Hz groups,with 8 cases in each group.Chronic neuropathic pain model was established by ligature of the left sciatic nerve under anesthesia(Urethane +Alpha-Chloralose) except rats in the control group.EA(2 Hz,2 Hz/15 Hz,100 Hz,1 mA) was applied to bilateral "Zusanli"(ST 36)-"Yanglingquan"(GB 34) for 30 min,once each day for 2 weeks.The thermal and mechanical paw withdrawal latencies(pain thresholds) of the bilateral limbs were detected before and after EA interventions.The hippocampal tissue of the rat was collected for detecting the expression levels of nNOS,iNOS and PKG genes using quantitative real-time-PCR technique.Results In comparison with the control group,the thermal and mechanical pain thresholds of the model group were decreased obviously(P<0.05).Compared with the model group,both thermal and mechanical pain thresholds of the EA-2 Hz,EA-2 Hz/15 Hz and EA-100 Hz groups were markedly increased after EA intervention for 3,7,10 and 14 days(P<0.05).Compared with the control group,the expression levels of hippocampal nNOS and PKG mRNA were significantly and moderately up-regulated in the model group(P<0.05).While in comparison with the model group,the expression levels of hippocampal nNOS and PKG mRNA in the EA-2 Hz,EA-2 Hz/15 Hz and EA-100 Hz groups were markedly down-regulated(P<0.05).No significant differences were found among the EA-2 Hz,EA-2 Hz/15 Hz and EA-100 Hz groups in the analgesic effect and in down-regulating hippocampal nNOS and PKG mRNA expression(P>0.05).However,the recovery state of the pain reaction of both EA-2 Hz and EA-2 Hz/15 Hz groups was relatively better than that of the EA-100 Hz group from day 3 to 10 after EA intervention.Conclusion EA stimulation of "Zusanli"(ST 36)-"Yanglingquan"(GB 34) at 2 Hz,2 Hz/15 Hz and 100 Hz can significantly suppress chronic neuropathic pain induced in CCI rats,which may be closely associated with its effects in down-regulating hippocampal nNOS and PKG mRNA expression levels.1532|430|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) at "Fenglong"(ST 40) on blood lipid levels and hepatic ATP binding cassette transporter A 1(ABCA 1) mRNA and protein expression in hyperlipidemia rats,so as to study its mechanism underlying improvement of HLP.Methods Forty SD rats were randomized into normal control,model,diet control,EA,and EA+diet control groups,with 8 rats in each group.Hyperlipidemia model was established by feeding the animals with high fat forage for 28 days.After modeling,rats of the two diet control groups were fed with basal forage.EA(2 mA,2 Hz/100 Hz) was applied to bilateral ST 40 for 30 min,once daily for 28 days.Plasma total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C) contents were detected by automatic biochemistry analyzer,and the expression levels of ABCA 1 mRNA and protein in the liver tissue were assayed by in situ hybri-dization(ISH),RT-PCR and Western blot,respectively.Results Compared with the normal control group,the contents of plasma TC and LDL-C were apparently increased(P<0.01) and the expression levels of hepatic ABCA 1 mRNA(detected by both ISH and RT-PCR) and ABCA 1 protein were significantly decreased in the model group(P<0.01).In comparison with the model group,plasma TC and LDL-C contents were significantly decreased and the expression levels of hepatic ABCA 1 mRNA and protein were significantly up-regulated in the EA group and EA+diet control group(P<0.01).The effects of EA+diet control were significantly superior to those of diet control in down-regulating plasma TC and LDL-C levels and up-regulating hepatic ABCA 1 mRNA and protein expression levels(P<0.01).Conclusion EA stimulation of "Fenglong"(ST 40) can decrease the load of blood cholesterol and suppress the down-regulation of hepatic ABCA 1 mRNA and protein expression in hyperlipidemia rats,which may contribute to its effect in improving hyperlipidemia.关键词：Hyperlipidemia;Electroacupuncture;ATP binding cassette transporter A 1;Gene and protein expression1321|248|3更新时间：2023-08-11
- 摘要：Objective To observe the influence of acupuncture intervention on expression of protein kinase A(PKA) in the cerebrocortex of rats with focal cerebral ischemia-reperfusion injury(CI/RI),so as to explore its underlying neuroprotection mechanism on cerebral ischemia.Methods Ninety male SD rats were randomly and evenly divided into sham-operation(sham),model and acupuncture groups which were further randomized into 3,7 and 14-days(d) subgroups(10 rats/subgroup).CI/RI model was established by occlusion of the left middle cerebral artery for 2 hours and reperfusion.Manual acupuncture stimulation was applied to "Baihui"(GV 20) and "Shuigou"(GV 26),as well as the right "Quchi"(LI 11),"Hegu"(LI 4),"Neiguan"(PC 6),"Zusanli"(ST 36),"Sanyinjiao"(SP 6) and "Taichong"(LR 3) for 30 min,once daily for 3,7 and 14 d respectively.Rats of the sham and model groups were restrained for 30 min each day.Neurological defects were assessed by ethologic scoring according to Bederson's neurologic assessment scales.Cellular apoptosis in the ischemic cortex was detected by flow cytometry and PKA expression determined by immunohistochemistry for calculating its PKA immunoreaction(IR)-positive cell rate,respectively.Results Compared with the sham group,the neurologic scores,cortical cellular apoptosis rates and PKA IR-positive cell rates of the model group were significantly increased at the time-points of day 3,7 and 14 post-treatment(P<0.05).In comparison with the model group,the neurologic scores and cortical cellular apoptosis rates of the acupuncture group at the time-points of day 3,7 and 14 post-treatment were considerably down-regulated(P<0.05),and the cortical PKA IR-positive cell rates of the acupuncture group were remarkably increased(P<0.05).In addition,along with the increase of acupuncture treatment sessions,lower apoptosis rates and more PKA IR-positive cells were found,suggesting a cumulative effect.Conclusion Acupuncture intervention can lower cellular apoptosis rate of the ischemic cerebrocortex and up-regulate cortical PKA expression level in CI/RI rats,which may be responsible for its effect in improving neurologic deficits.关键词：Cerebral ischemia-reperfusion;Acupuncture intervention;Ischemic cerebrocortex;Protein kinase A;Cellular apoptosis;Neurologic deficits1449|430|7更新时间：2023-08-11
- 摘要：Objective To observe the influence of electroacupuncture(EA) stimulation of "Hegu"(LI 4) and "Sanyinjiao"(SP 6;a classical delivery-facilitating acupoint recipe) at different parameters on endocrine hormone levels of late-stage pregnant rats,so as to find the better stimulation parameters for delivery facilitation.Methods Ninety-six female Wistar rats were randomly divided into eight groups: normal control(normal),pregnancy(model),EA-15 Hz,EA-30 Hz,EA-50 Hz,EA-2 Hz/15 Hz,EA-2 Hz/30 Hz and EA-2 Hz/50 Hz,with 12 rats in each group except EA-30 Hz group(n=10).EA stimulation(0.2 to 0.3 mA) was delivered to bilateral LI 4 for 20 min and then bilateral SP 6 for 5 min(25 min).The contents of serum estradiol(E2),progesterone(P) and prostaglandin E2(PGE2) were detected by ELISA.Results Compared with the normal group,se-rum E2,P and PGE2 contents were significantly increased in the pregnancy model group(P<0.01),and E2 / P level was down-regulated mildly(P>0.05).Compared with the model group,the contents of serum E2 in the EA-15 Hz,EA-30 Hz,EA-50 Hz,EA-2 Hz/15 Hz and EA-2 Hz/50 Hz groups,serum PGE2 contents in the EA-15 Hz,EA-30 Hz and EA-2 Hz/50 Hz groups,and E2/P levels in the EA-15 Hz,EA-30 Hz,EA-50 Hz and EA-2 Hz/50 Hz groups were significantly increased(P<0.01,P<0.05),whereas serum P contents in the EA-15 Hz,EA-30 Hz,EA-50 Hz and EA-2 Hz/50 Hz groups were significantly down-regulated(P<0.01,P<0.05).The effects of EA-2 Hz/50 Hz group were significantly superior to those of EA-2 Hz/15 Hz and EA-2 Hz/30 Hz groups in raising serum E2 and E2/P levels(P<0.05,P<0.01).No significant differences were found among the EA-15 Hz,EA-30 Hz,EA-50 Hz and EA-2 Hz/50 Hz groups in raising serum E2,PGE2 and E2/P levels,and among the EA-30 Hz,EA-50 Hz and EA-2 Hz/50 Hz groups in lowering serum P levels(P>0.05).It displayed that the effects of EA at 15 Hz,30 Hz and 2 Hz/50 Hz on the abovementioned 4 indexes were relatively better.Conclusion EA of LI 4 and SP 6 at 15 Hz,30 Hz and 2 Hz/50 Hz can effectively up-regulate serum E2,PGE2 and E2/P levels and down-regulate serum P content in pregnancy rats,which may contribute to its effect in promoting labor.关键词：Labor promotion;Electroacupuncture;Hegu(LI 4);Sanyinjiao(SP 6);Stimulating frequency;Serum estradiol;Progesterone;Prostaglandin E21414|360|2更新时间：2023-08-11
- 摘要：Objective To observe changes of discharges of the sciatic nerve branch and mast cell activities and collagen fibers in the acupoint area during manual acupuncture stimulation of "Zusanli"(ST 36),so as to reveal the relationship between peripheral nerve and mast cell activities.Methods A total of 30 male SD rats were divided into normal,acupuncture control(an acupuncture needle was inserted into ST 36 without manipulation),manual acupuncture(MA),disodium cromoglycate(DSCG,suppressing mast cell activity) plus acupuncture(MA+DSCG) and collagenase(dissolving the collagen fibers) plus acupuncture(MA+collagenase) groups(6 rats/group).After dissection of a branch of the sciatic nerve innervating ST 36 region in the left hind-limb under anesthesia,the ipsilateral ST 36 was stimulated by manipulating the acupuncture needle for 20 min.Discharges of the sciatic nerve branch were recorded by using a pair of metal electrodes and data acquisition system(Power Lab).Skin and muscle tissues of ST 36 area were sampled,sliced and stained with Toluidine Blue for detecting the number of degranulated mast cells.Results Compared with the control group,the mean power spectrum of discharges of the sciatic nerve and the mean rates of the degranulated mast cells in "Zusanli"(ST 36) area in the MA group were significantly increased(P<0.01).Whereas the mean power spectrum of discharges of the sciatic nerve and the mean degranulation rates of mast cells were consi-derably lower in the MA +DSCG group and MA+collagenase group than in the MA group(P<0.01).No significant differences were found between the normal and control groups,and between the MA+DSCG and MA+collagenase groups in the mean power density and degranulation rates of mast cells(P>0.05).Conclusion Manual acupuncture stimulation of ST 36 can significantly potentiate the discharge activity of the sciatic nerve and induce degranulation of mast cells at the same time,suggesting an involvement of mast cells in initiating acupuncture signals by peripheral sensory nerve.关键词：Acupuncture;Mast cells;Disodium cromoglycate;Collagenase;Peripheral nerve discharges1426|791|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) of "Neiguan"(PC 6) and "Xinshu"(BL 15) on levels of serum total cholesterol(TC),triglyeride(TG),high density lipoprotein(HDL) and low-density lipoprotein(LDL) and expression of T cell CD 40 L and matrix metalloproteinases-9(MMP-9) in the coronary artery in coronary heart disease(CHD) rats,so as to reveal its mechanism underlying improvement of coronary atherosclerotic heart disease.Methods A total of 60 Wistar rats were randomly divided into normal control,model,EA-pretreatment,routine EA and medication groups(n=12).Co-ronary atherosclerotic heart disease model was established by feeding the animals with high fat forage for 3 months and intrape-ritoneal injection of VD3(2 mL/kg,60 000 IU/100 g,once daily for 3 days).EA was applied to unilateral "Neiguan"(PC 6) and "Xinshu"(BL 15) once every other day for rats of the EA-pretreatment group from the 1st day of modeling on for 12 weeks,and once daily for rats of the routine EA group from successful modeling on for 2 weeks.Rats of the medication group were treated by intragastric administration of Atorvastatin 0.25 mg·kg-1·d-1 for 2 weeks.Serum TC,TG,HDL and LDL contents were detected by ELISA and the expression levels of CD 40 L and MMP-9 of the coronary artery tissue assayed by Western blot.Results Serum contents of TC,TG and LDL and expression levels of coronary arterial CD 40 L and MMP-9 in the model group were significantly higher than those of the normal control group(P<0.01),while serum HDL content was decreased considerably in the mo-del group(P<0.01).In comparison with the model group,the contents of serum TC,TG,HDL and the expression levels of arterial CD 40 L and MMP-9 in both EA-pretreatment group and routine EA group were remarkably down-regulated(P<0.01),and serum LDL content was obviously up-regulated(P<0.01).The effects of the EA-pretreatment group were significantly superior to those of the medication group in down-regulating the contents of serum TC,TG,HDL and decreasing the expression of coronary arterial CD 40 L and MMP-9 proteins(P<0.01,P<0.05).No significant differences were found between the EA-pretreatment and routine EA groups in all the 6 indexes(P>0.05).Conclusion Both EA-pretreatment and routine EA of PC 6 and BL 15 can down-regulate blood TC,TG and HDL contents,raise serum HDL level and suppress the increased expression of CD 40 L and MMP-9 proteins of coronary artery tissue in rats with coronary atherosclerotic heart disease,which may contribute to their effect in improving CHD.1636|879|12更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion intervention on inflammatory reactions and expression of suppressor of cyfokine signaling 1(SOCS 1) and SOCS 2 [Which are involved in inhibition of the Janus Kinase-signal transducer and activator of transcrip-tion(JAK/STAT signaling pathway and in sffenuation of cytokine signaling)] in synovium cells of the hind-knee joint in rheumatoid arthritis(RA) rabbits,so as to study its mechanism underlying improvement of RA.Methods Forty-two Japanese big-ear white rabbits were randomized into control,model and moxibustion groups respectively,with 14 cases in each group.RA model was established by injection of Freund's Complete Adjuvant(0.5 mL/kg) into the rabbits' bilateral hind-knee joint cavities.Moxibustion was applied to bilateral "Shenshu"(BL 23) areas,5 cones every time,once daily for 3 weeks except the Sundays.The perimeters of rabbits' hind legs were measured before and after modeling and after the therapy.The synovial tissue of joint was sampled for analyzing the expression levels of SOCS 1 and SOCS 3 by immunohistochemistry.Results Before the therapy,the perimeters of bilateral knee joints of the control,model and moxibustion groups were of no statistical significance(P>0.05).In comparison with the control group,the perimeters of bilateral knee joints were significantly increased on day 1,7,14 and 21 in the model group(P<0.01).Compared with the model group,the perimeters of bilateral knee joints in the moxibustion group were significantly decreased(P<0.05),suggesting an improvement of the inflammatory reaction after moxibustion intervention.Correspondingly,synovial SOCS 1 and SOCS 3 expression levels were remarkabely higher in the model group than in the control group(P<0.01),and obviously decreased in the moxibustion group compared with the model group(P<0.01).Conclusions Moxibustion intervention has an anti-inflammatory and detumescent effects in RA rabbits,which may be closely associated with its effects in down-regulating expression of SOCS 1 and SOCS 3 proteins by suppressing negative feedback regulatory JAK/STAT pathway in synovial cells.关键词：Moxibustion;Rheumatoid arthritis;Inflammatory reactions;Synovial cells;Suppressor of cytokine signaling proteins;Negative-feedback regulatory factors1438|936|14更新时间：2023-08-11
- 摘要：Objective To observe the effect of different moxibustion intervention on expression of interleukin-1(IL-1) and tumor necrosis factor-α(TNF-α) in the synovial fluid of hind-knee joint in rheumatoid arthritis(RA) rabbits.Methods Forty Japanese big-ear white rabbits(half male and half female) were randomized into normal control,RA model,direct-moxibustion,ginger-partitioned moxibustion and warm moxibustion groups(n=8).RA model was established by injection of Freund's Complete Adjuvant(0.5 mL/kg) into the articular cavities of the rabbits' bilateral hind-limbs.Moxibustion intervention was applied to unilateral "Shenshu"(BL 23) and "Zusanli"(ST 36) regions alternatively for 20 min from the 7thday on after modeling,once daily for 3 weeks except Sundays.The circumference of the hindlimb-knee joint was measured using a tape measure and the contents of IL-1 and TNF-α in the synovial fluid of articular cavities were detected by ELISA.Results In comparison with the normal control group,the circumference values of the bilateral hind-knee joints,and the contents of IL-1 and TNF-α in the synovial fluid of articular cavities in the model group were significantly increased(P<0.01).After the moxibustion treatment,compared with the model group,the circumference values of the bilateral hind-knee joints,and IL-1 and TNF-α contents of the synovial fluid in the warm moxibustion,direct moxibustion and ginger-partitioned moxibustion groups were remarkably reduced(P<0.01,P<0.05).The effects of the ginger-partitioned group were significantly superior to those of both warm moxibustion and direct moxibustion groups in decreasing the swelled hind-knee joint circumference on day 21 after the treatment and down-regulating synovial fluid inflammatory cytokines IL-1 and TNF-α levels(P<0.05).Conclusion Warm,direct and ginger-separated moxibustion interventions all can reduce inflammatory reactions of the knee-joint and suppress inflammatory cytokine IL-1 and TNF-α levels of the synovial fluid in RA rabbits,which may contribute to its effect in improving RA in clinic.The therapeutic effect of ginger-partitioned muxibustion intervention is apparently better.关键词：Rheumatoid arthritis;Ginger-partitioned moxibustion;Warm moxibustion;Direct moxibustion;Inflammatory reactions;Synovial fluid IL-1;TNF-α1531|1030|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on behavior reactions and hippocampal glucocorticoid receptor(GR) and mineralocorticoid receptor(MR) protein expression in rats with post-traumatic stress disorder(PTSD),so as to explore its mechanism underlying improvement of PTSD.Methods Thirty male Sprague-Dawley rats were randomly divided into control,model and EA groups(10 rats/group).PTSD model was established by single prolonged stress.EA(2 Hz,1 mA) stimulation was applied to "Baihui"(GV 20) and bilateral "Zusanli"(ST 36) acupoints for 30 min,once a day for a week.Morris water maze test was used to measure the ability of spatial learning and memory.GR and MR protein expression le-vels in the hippocampus tissue were examined with immunohistochemistry.Results Compared with the control group,the animals' escape latency and the expression level of hippocampal GR protein were increased significantly,and the expression level of hippocampal MR protein,as well the ratio of MR/GR expression were down-regulated considerably in the model group(P<0.05).In comparison with the model group,the animals' escape latency and the expression level of hippocampal GR protein were shortened pronouncedly,and the expression level of hippocampal MR protein,as well the ratio of MR/GR immunoactivity were up-regulated considerably(P<0.05).Conclusion EA of GV 20 and ST 36 can improve the learning-memory ability and regulate hippocampal MR and GR expression in PTSD rats,which may contribute to its effect in relieving PTSD in clinical practice.关键词：Post-traumatic stress disorder;Electroacupuncture;Hippocampus;Glucocorticoid receptor;Mine-ralocorticoid receptor;Learning-memory ability1081|505|8更新时间：2023-08-11
- 摘要："Mingmen"(GV 4) is one of the most frequently used acupoints in acupuncture clinic.In recent years,more and more experimental researches have been focusing on GV 4 or acupoint recipe containing GV 4 in rats.Accurate location of GV 4 is probably not only related to fully display its therapeutic effect,but also to help study its underlying mechanisms.However,there has been no unified standard about the accurate location of GV 4 in the adult rat at present.In the present paper,the authors review related literature about GV 4 location in experimental studies in recent 10 years,and put forward a practical method for locating GV 4 in the rat by combining their own experience.That is,GV 4 is taken according to the relative relationship of ilium and spinous process of the lumbar vertebra.In addition,the authors also recommend some matters needing attention in locating GV 4 in rats.关键词：Mingmen(GV 4);Rats;Locating method1210|318|4更新时间：2023-08-11
- 摘要：Objective To observe the clinical therapeutic effect of ear-apex blood-letting for external sty.Methods A total 102 sty patients were randomized into ear-apex blood-letting group(n=51) and routine treatment(medication) group(n=51) according to computer-aided randomization procedure.Ear-apex-bloodletting(5-6 blood drops/time) was performed once daily for 3 times for the patients of blood-letting group.Patients of the medication group were treated by local application of hydrochloric levofloxacin and erycin ointment to the affected eyelid lining.Additionally,local warm compress of the affected eyelid was given to patients of both groups.The therapeutic effect was assessed by measuring the size of the sty swell and visual analogue scale(VAS) was determined for evaluating pain severity changes.The outcomes were analyzed by researchers who did not know the grouping.Results Comparison between patients of the two groups showed that the difference vahees of the styrize and VAS score between pre-and post-treatment in the ear-apex bloodletting group were significantly bigger than those of the me-dication group on day 3,5 and 7 after treatment(P<0.05).The cure rates of the blood-letting group and medication group were 64.7% and 41.2%,90.2% and 62.7%,94.1% and 80.4%,respectively on day 3,5 and 7 after the treatment.The therapeutic effects of blood-letting were significantly superior to those of the medication group in relieving external sty(P<0.05).Conclusion Ear-apex blood-letting therapy for external sty is effective in relieving pain,reducing the size and shortening the duration of disease.关键词：External sty;Ear-apex blood-letting;Conventional treatment;Randomized controlled trial1016|629|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of "Sancai Needling"(superficial,medium and deep insertion of the acupuncture needle) combined with electroacupuncture(EA) stimulation of Shenshu(BL 23) on plasma and urine SP and 5-HT levels in renal colic patients,so as to study its mechanism underlying improvement of renal colic.Methods Sixty renal colic patients(lower-energizer stasis type) were randomly allocated to "Sancai Needling"-EA group,Ashi-point-EA group and routine EA treatment group,with 20 cases in each group.The "Sancai Needling"-EA meant that an acupuncture needle was inserted into the subcutaneous layer of BL 23 first and EA stimulation was given for 10 min;then the needle was inserted into the medium layer(muscle layer) and EA stimulation performed for 10 min;at last,the needle was further inserted into the periosteum-muscle layer and EA was performed for 10 min again.The procedures for Ashi-point-EA group were the same.For patients of the routine EA treatment group,EA was applied to BL 23 for 30 min.The therapeutic effect for pain was assessed according to McGill Pain Questionnaire.Plasma and urine SP and 5-HT contents were determined using enzyme-labeled immunosorbent assay(ELISA).Results In comparison with pre-treatment,pain scores,plasma and urine SP and 5-HT contents were remarkably decreased in renal colic patients of the "Sancai Needling"-EA group,Ashi-point-EA group and routine EA treatment group after the treatment(P<0.05).The effects of the "Sancai Needling"-EA group and Ashi-point-EA group were significantly superior to those of the routine EA treatment group in reducing pain score,plasma and urine SP and 5-HT contents(P<0.05).No statistical differences were found between the "Sancai Needling"-EA group and Ashi-point-EA group in pain score,plasma and urine SP and 5-HT contents(P>0.05).Conclusion "Sancai Needling"-EA treatment is effective in relieving renal colic in the patients,which may be closely associated with its effects in down-regulating plasma SP and 5-HT levels.关键词：Electroacupuncture therapy;Renal colic;"Sancai Needling";Substance P;5-HT1153|293|1更新时间：2023-08-11
- 摘要：Objective To observe the arousal effect of electroacupuncture(EA) stimulation of Baihui(GV 20),Shuigou(GV 26),etc.on severe craniocerebral injury patients.Methods A total of 90 cases of severe craniocerebral injury were randomly allocated to routine medication,naloxone and EA groups,with 30 cases in each group.For patients of the routine medication group,mild hypothermia therapy,medicines for dehydration,hormonal therapy,vascular dilation,cerebral nutrition suppor-ting,anti-inflammation,etc.were given.For patients of the naloxone group,intravenous drip of naloxone 0.4 mg/kg in the first 3 days,0.2 mg/kg for 7 days and 0.1 mg/kg afterwards.For patients of the EA group,EA(1 Hz/50 Hz) was given for 30 min once daily.All the treatments were conducted once a day for 14 days.The Glasgow Coma Scale(GCS) and Glasgow Outcome Scale(GOS) were used for assessing the therapeutic effect.Results In comparison with pre-treatment in each one of the routine medication,naloxone and EA groups,GCS scores were all obviously increased in the 3 groups following the treatment,and one month's follow-up(P<0.05).The GCS scores of both naloxone and EA groups were significantly higher than those of the routine medication group(P<0.05).No significant difference was found between the naloxone group and EA group in GCS scores(P>0.05).According to the GOS,one month's follow-up showed that of the three 30 cases in the routine medication,naloxone and EA groups,6,12 and 14 were improved,8,10 and 10 moderate handicap,8,3 and 2 severe handicap,5,3 and 2 vegetative state,and 3,2 and 2 dead,with the arousal rates being 46.66%,73.33% and 80.00%,respectively.The therapeutic effects of both naloxone and EA groups were significantly superior to those of the routine medication group(P<0.05).Conclusion EA intervention at early stage can promote the recovery of neurological function,accelerate the consciousness from coma and improve the outcomes of patients with severe traumatic brain injury.关键词：Craniocerebral injury;Coma;Electroacupuncture intervention;Arousal rate;Glasgow Coma Scale;Glasgow Outcome Scale1276|752|10更新时间：2023-08-11
- 摘要：In the present article,the authors made an overview about the existing problems of placebo acupuncture design in accordance with the neurological basis of acupuncture analgesia.The neuron-segmental and systemic mechanisms initiated by local somesthetic stimuli at different intensities are involved in acupuncture analgesia.When the local pain locus and the stimulated point are in the same spinal segmental region,stimuli of either higher intensity or lower intensity may produce an obvious ana-lgesia effect.If the stimulation site is far from the pain locus(in remote spinal segment region),only higher intensity stimulation works.From this viewpoint,the placebo acupuncture design in current clinical trials for pain treatment exists some unreasonable aspects.Both pain focus and intensity of acupuncture stimulation should be taken into consideration together.The optimal placebo acupuncture design for the treatment of pain conditions is that lower intensity acupuncture stimulation is given for longer distance between the pain origin locus and the stimulated acupoint.关键词：Placebo acupuncture design;Acupuncture analgesia;Neurological basis;Higher intensity acupuncture stimulation;Weaker acupuncture stimulation1540|983|7更新时间：2023-08-11
- 摘要：Deqi(needle sensation),is closely related to clinical therapeutic effect of acupuncture in the treatment of different clinical conditions.In the present paper,the authors summarized various components or concepts of Deqi mainly in the fo-reign studies collected from Pubmed database.At present,foreign researches about Deqi mainly include 1) the subjects' subjective qualitative and quantitative descriptions about Deqi,2) correlation between acupuncture stimulation induced Deqi and clinical therapeutic effects,and 3) responses of different brain regions or the connectivity of brain network shown by functional magnetic resonance imaging(fMRI) during Deqi by needling different acupoints.According to the current commonly used 6 questionnaires,aching,tingling,numbness,heaviness,dull pain,throbbing,and deep pressure sensation are the most frequently seen components of Deqi of acupuncture stimulation.The factors influencing Deqi are psychological state,personal previous experience and cultural background.Regarding the degree of Deqi and clinical effects or outcomes,the conclusions are controversial.Results of fMRI analysis shows that in spite of extensive deactivation of the limbic system has been found in many studies,and the connectivity of the intrinsic brain functional networks is increased during acupuncture-induced Deqi,the significance of activation or deactivation of some brain regions remains unclear,and the related mechanisms need to be studied further.No matter activation or dea-ctivation of different brain regions during Deqi,the most important issue is the relationship between Deqi and clinical therapeutic effects.Further studies are definitely needed.关键词：Acupuncture therapy;Needling sensations;Deqi;Qualitation;Quantization;Clinical effect;Functional magnetic resonance imaging1474|1394|5更新时间：2023-08-11
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