最新刊期
卷 39 ， 期 1 ， 2014
- 摘要：Objective To determine whether electroacupuncture(EA)intervention combined with general anesthesia(GA)strategy can reduce early post-operative morbidity and medical costs in patients undergoing heart valve replacement operation under cardiopulmonary bypass.Methods A total of 160heart valve replacement surgery patients undergoing cardiopulmonary bypass were randomly divided into GA and EA+GA groups(n=80in each group).Patients of the GA group were given with intravenous injection of Fentanyl,Midazolam,Vecuronium Bromide,etc.and routine tracheal intubation.EA(3-4Hz,2.0-2.2mA)was applied to bilateral Zhongfu(LU 1),Chize(LU 5)and Ximen(PC 4)beginning about 20min before the surgery in the EA+GA group.Endotracheal intubation was not employed but only prepared as a standby for patients of the EA+GA group.The dosage of narcotic drugs,duration of surgery,duration of aortic blockage,rate of cardiac re-beating,volumes of post-operative blood transfusion,discharge volume,cases of post-operative pulmonary infection,vocal cord injury,and the time of first bedoff,first eating and duration in intensive care unit(ICU)residence,etc.were recorded.Results The successful rates of heart valve replacement surgery were similar in both GA and EA+GA groups.Compared with the GA group,the dosages of Fentanyl,Midazolam and Vecuronium of the EA+GA group were significantly lower(P<0.05,P<0.01),the numbers of patients needing blood-transfusion,antibiotics treatment,and suffering from pulmonary infection were fewer,the time of first bed-off and duration of hospitalization and ICU residence were considerably shorter(P<0.05,P<0.01)and the total medical cost was obviously lower(P<0.05)in the EA+GA group.Conclusion EA combined with general anesthesia strategy for heart valve replacement surgery without endotracheal intubation is safe and can reduce post-operative morbidity and medical costs in patients undergoing heart valve replacement surgery under cardiopulmonary bypass.关键词：Heart valve replacement operation;Electroacupuncture combined with general anesthesia;General anesthesia;Non-endotracheal intubation;Post-operative morbidity;Medical cost1302|423|5更新时间：2023-08-11
- 摘要：Objective To observe the protective effect of transcutaneous electrical acupoint stimulation(TEAS)on cerebral tissue in elderly hip replacement operation patients during general anesthesia under controlled hypotension.MethodsForty hip replacement operation patients were randomly divided into general anesthesia(GA)control group and TEAS+GA group(n=20in each group).Patients of the two groups during operation were treated with controlled hypotension for reducing blood loss.TEAS(2Hz/100Hz,8-12mA)was applied to bilateral Yuyao(EX-HN 4)and Fengchi(GB 20)and began 30min before GA.General anesthesia was performed by intravenous injection of Midazolam,Diprivan,Fentanyl and Cis-atracurium,sevoflurane-inhaling,Remifentanil,etc.,and the patient's mean arterial pressure was maintained to be about 70% of the normal level(controlled hypotension)by using venous administration of Remifentanil about 10min after the operation.GEM Premier 3000blood gas analyzer was used to analyze levels of the arterial oxygen(CaO2),internal jugular venous oxygen(CjvO2),arterio-venous oxygen content difference(Da-jvO2)and cerebral oxygen(CERO2)uptake rates of blood samples before controlled hypotension(T0),20min after controlled hypotension(T1),40min after controlled hypotension(T2)and 20min after the end of controlled hypotension(T3).Results Self-comparison of each group showed that in comparison with pre-controlled hypotension,CjvO2levels at the time-points of T1,T2and T3 were significantly increased in both GA control and TEAS+GA groups(P<0.05),while Da-jvO2and CERO2 uptake rate levels at the time-points of T1,T2 and T3in these two groups were significantly decreased(P<0.05).Comparison between two groups showed that CjvO2levels at the time-points of T2and T3were significantly higher in the TEAS+GA group than in the GA control group(P<0.05),while Da-jvO2and CERO2uptake rate levels at the time-point of T2were obviously lower in the TEAS+GA group than in the GA control group(P<0.05),suggesting a reduction of cerebral oxygen consumption after TEAS.No significant differences were found between these two groups in blood CjvO2,Da-jvO2and CERO2uptake rate levels at the time-points of T0and T1(P>0.05).Conclusion TEAS can reduce cerebral oxygen uptake rate in elderly patients undergoing hip replacement during controlled hypotension,suggesting aprotective effect of TEAS on patient's cerebral tissue.关键词：Artificial hip replacement;Transcutaneous electrical acupoint stimulation;Controlled hypotension;El-derly patients;Cerebral oxygen uptake rate;Cerebral protection1586|318|2更新时间：2023-08-11
- 摘要：Objective To determine the safety and effectiveness of electroacupuncture(EA)intervention combined with general anesthesia(GA)for craniocerebral tumor-removal surgery involving cerebral eloquent areas.Methods A total of 20patients with cerebral tumors(durosarcoma,neurogliocytoma,metastatic tumor)involving the cerebral eloquent areas were recruited in the present summary.They were equally divided into general anesthesia(GA)group and EA+GA group.Patients of the EA+GA group were treated with EA stimulation(2Hz/100Hz)of the bilateral Fengchi(GB 20),Shuaigu(GB 8)and acupuncture stimulation of Quanliao(SI 18),Zulinqi(GB 41)and Taichong(LR 3),and not given intubation during surgery.Patients of the GA group were treated with intravenous administration of Propofol,Fentanyl,Midazolam,Isopropanol,etc.In addition,all the patients were given with controlled hypotension by intravenous injection of Propofol and Fentanyl.Dosages of Propofol and Sulfentanyl used were recorded.Karnofsky performance status scale was used to assess the patient's general well-being and activities of daily life.Results The craniocerebral tumor-removal surgery was successful in all the 20patients.Compared with the GA group,the dosages of Sulfentanyl and Propofol of the EA+GA group were significantly lower(P<0.05).No significant difference was found between the GA and EA+GA groups in Karnofsky performance scores(P>0.05).Patients of the EA+GA group experienced successful tumour-removal surgery without trachea cannula,were awake and could make a good cooperation with the operator during surgery.They had no aggravation of neurofunctional disturbance following the operation.Conclusion EA combined with general anesthesia is safe and effective for patients with craniocerebral tumor-removal operations involving cerebral eloquent areas.关键词：Acupuncture-aided general anesthesia;Craniocerebral tumor-removal operation;Cerebral eloquent areas;Non-tracheal intubation1412|233|3更新时间：2023-08-11
- 摘要：Objective To summarize post-operative management strategy for heart valve replacement surgery under transcutaneous electrical acupoint stimulation(TEAS)combined with general anesthesia.Methods From July 2006to June2012,a total of 86cases of open-heart surgery patients experiencing TEAS plus general anesthesia with cardiopulmonary bypass(heart valve replacement surgery without intubation)were recruited in the present summary.Post-operative managements in the Intensive Care Unit(ICU)were administrated with strict hemodynamic monitoring for volume infusion,routine administration of vasoactive drugs(including dopamine and nitroglycerin),oxygen inhalation,and analgesics and monitoring of central nervous system and renal function.Results All the 86patients under TEAS+general anesthesia and cardiopulmonary bypass and without intubation experienced successful heart valve replacement surgery.The post-operative pulmonary infection was found in 8cases(9.30%),the average stay duration in ICU was(28.6±6.2)hours,and the average draining volume of the interpleural space was(291±73)mL.The cardia insufficiency was found in 5cases(5.81%),hypoxia occurred in 8cases(9.30%),nausea in 8cases(9.30%),vomiting 5cases(5.81%)and post-operative gastrointestinal distension 13cases(15.12%),mild renal insufficiency 3cases(3.49%),fever(>38.5℃)1case(1.16%)and severe post-operative pain 7cases(8.14%).ConclusionTEAS combined with general anesthesia is safe for patients undergoing heart valve replacement surgery under cardiopulmonary bypass.The key points of treatment in ICU are volume infusion,body temperature maintenance,and pain control.关键词：Acupuncture-anesthetics compound anesthesia;Heart valve replacement operation;Non-intubation surgery;Post-operation complications875|243|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion at different duration on colonic mucosal morphological changes,serum and colonic cytokine levels in ulcerative colitis(UC)rats,so as to provide experimental evidence for clinical treatment of UC.Methods SD rats were randomly divided into blank control,UC model,3cones-moxibustion(3-cones-M),6-cones-M and 9-cones-M groups,with 6rats in each group.UC model was established by intra-rectal injection of mixture solution of5%trinitro-benzene-sulfonic acid(TNBS,100mg/kg)and 50% alcohol(1∶1)under anesthesia and oral administration of 5%dextran sodium sulfate.Moxibustion(ignited moxa cones)was applied to"Tianshu"(ST 25)and"Daheng"(SP 15),once daily in the first 7days,and once every other day in the subsequent 14days.Serum IL-8and IL-10contents were assayed by ELISA and colonic toll-like receptor 9(TLR-9)and nuclear factor-κB p 65(NF-κB p 65)protein expression levels detected by Western blot.The colonic mucosal structure was observed by light microscope after H.E.staining,and by electron microscope,respectively.Results In comparison with the blank control group,the Disease Activity Index(DAI),serum IL-8content,colonic TLR-9and NF-κB p 65protein expression levels were significantly increased in the model group(P<0.05),and serum IL-10content was notably decreased in the model group(P<0.05).While in comparison with the model group,the DAI,serum IL-8content,colonic TLR-9and NF-κB p 65protein expression levels in the 3-cones-M,6-cones-M and 9-cones-M groups were remarkably down-regulated(P<0.05),and serum IL-10contents considerably up-regulated in the three moxibustion groups(P<0.05).No significant differences were found among the three moxibustion groups in the DAI(P>0.05).The serum IL-8contents were significantly lower and serum IL-10contents were considerably higher in the 6-cones-M and 9-cones-M groups than in the 3-cones-M group(P<0.05).The changes of colonic TLR-9and NF-κB p 65protein expression were more remarkable in the 9-cones-M group than in the 3-cones-M and 6-cones-M groups(P<0.05).Results of H.E.staining and electron microscopy showed that in the model group,mucosal injury,partial disorganization of the glandular organ,edema and congestion and inflammatory cell infiltration,mucosal epithelial microvili injury with disordered arrangement,etc.under light microscope,and local mucosal defect,apoptotic bodies and mucolysis under electron microscope were found,these situations were obviously lighter in rats of the three moxibustion groups,particularly in the 9-cones-M group.Conclusion Moxibustion intervention can relieve colonic mucosal injury in UC mice,which may be closely associated with its effects in suppressing serum proinflammatory cytokine IL-8,up-regulating anti-inflammatory cytokine IL-10level,and inhibiting colonic NF-κB p 65and TLR-9protein expression,and the effects of longer duration of moxibustion are better.1446|736|12更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)on enteric microbial populations in ulcerative colitis(UC)model rats,so as to study its mechanisms underlying improving UC.Methods Thirty-nine male SD rats were randomly divided into control group,model group and EA group(n=13in each group).The UC model was established by intragstric perfusion of 4%dextran sodiumaqueous solution(DSS),once daily for 7days.EA(15-25Hz,0.1mA)was applied to bilateral"Tianshu"(ST 25),"Zusanli"(ST 36)and"Shangjuxu"(ST 37)for 10min,once daily for 5days.Disease Activity Index(DAI)including body weight and stool blood state was used to evaluate the animals' conditions.The rats' excrement was collected to detect the abundance and diversity of bacterial populations by polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE).Results In comparison with the control group,the animals' DAI score and enteric Clostridium bifermentans content were significantly increased(P<0.05),and the abundance and diversity of bacterial populations,and contents of enteric Lachnospiraceae bacteriumand Lactobacillus sp.were considerably down-regulated in the model group(P<0.05,P<0.01).Compared with the model group,the DAI score and enteric Clostridium bifermentans content were significantly lowered,and the abundance and diversity of bacterial populations,and contents of enteric Lachnospiraceae bacteriumand Lactobacillus sp.were notably increased in the EA group(P<0.05,P<0.01).Conclusion EA can improve DAI and the abundance and diversity of enteric bacterial microbiota in UC rats,which may be related to its protective effect on enteric microecology in UC.关键词：Ulcerative colitis;Electroacupuncture intervention;Enteric microecology;Polymerase chain reaction-denaturing gradient gel electrophoresis1578|1539|21更新时间：2023-08-11
- 摘要：Objective To observe the effect of chronic stress stimulation on aging and the effect of acupoint catgut-embedding therapy on structural changes of hippocampal pyramidal neurons in senile chronic stress rats,so as to reveal its mechanisms underlying resisting senility.Methods Forty-eight male SD rats were randomly divided into normal control,senility model,senility+stress and senility+stress+catgut-embedding(catgut-embedding)groups(n=12in each group).Senility model was established by intraperitoneal injection of D-galactose liquor,once daily for 8weeks;and chronic stress was produced by restricting the rat in an inmovable cylinder for 1-4hprogressively,once daily for 4weeks.Catgut embedment(0.4-0.6cm in length)was applied to"Baihui"(GV 20)and"Shenshu"(BL 23),"Neiguan"(PC 6)and"Ganshu"(BL 18)alternatively by using a surgical needle,once a week,8weeks altogether.The brain tissue containing hippocampus was cut into sections to be stained with H.E.technique,and the number of the hippocampal pyramidal neurons of CA 3region was counted under optical microscope.The ultrastructure of pyramidal neurons in the left CA 3region of the hippocampus was observed by electron microscope(2rats for each group).Results Compared with the normal control group,the numbers of hippocampal pyramidal neurons in the senility model group and senility+stress group were remarkably decreased(P<0.05,P<0.01).The number of pyramidal neurons was obviously fewer in the senility+stress group than in the senility model group(P<0.01).In comparison with the senility+stress group,the number of pyramidal neurons in the catgut-embedding group was considerably increased(P<0.01).Results of optical microscope showed that the pyramidal cells were sparse in the senility model and very sparse in the senility+stress group,and relatively richer in the catgut-embedding group.Findings of the electron microscope displayed that the pyramidal neurons presented an injured state including shrank soma,pyknosis of the cellular nucleus,atrophia of nucleoli,onglobation of intranuclear caryotin,decreased glycogen granules,etc.in the senility model group,and an extremely irregular shape,partial apoptosis,severer pyknosis of the cellular nucleus,more edema blank regions,etc.in the senility+stress group.These situations of pyramidal cells in the hippocampal CA 3region were relatively milder in the catgut-embedding group.Conclusion Chronic stress may aggravate the damage of hippocampal pyramidal neurons in senile rats,which can be improved by catgut-embedding therapy.关键词：Senility;Chronic stress;Catgut-embedding therapy;Hippocampal neurons1442|238|2更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)of"Baihui"(GV 20)and"Zusanli"(ST 36)and EA plus polysaccharide of Gastrodia elata Blume(PGB)on neural stem cell(NSC)proliferation in cerebral ischemia(CI)rats,so as to explore its mechanisms underlying improvement of CI.Methods A total of 40SD rats were randomly divided into normal control,CI model,EA,medication(PGB)and EA+medication groups(n=8in each group).The CI model was established by occlusion of the middle cerebral artery(MCAO).Two weeks after modeling,rats of the medication and EA+medication groups were administrated with PGB(100mg/kg)once daily for 2successive weeks.EA(2Hz,3V)was applied to"Baihui"(GV 20)and left"Zusanli"(ST 36)for 30min,once daily for 2weeks.The expression of Nestin and stem cell factor(SCF)proteins in the dentate gyrus(DG)of hippocampus was detected by immunohistochemistry.Results Compared with the normal control group,the expression of Nestin and SCF proteins in the suprapyramidal blade(SPB)and infrapyramidal blade(IPB)of DG were significantly increased in the model group(P<0.05).After EA intervention,the expression levels of Nestin and SCF of SPB and IPB of DG were considerably further up-regulated in both EA group and EA+medication group(P<0.05),and the effect of EA+medication group was significantly superior to that of simple EA group and medication group(P<0.05).Conclusion EA combined with PGB can significantly up-regulate the expression of Nestin and SCF in the hippocampal DG in CI rats,and its effects are apparently superior to those of simple EA or simple medication.It suggests that EA-induced proliferation of SCF may be one of the mechanisms underlying relieving CI.关键词：Cerebral ischemia;Electroacupuncture;Polysaccharide of Gastrodia elata Blume;Nestin;Stem cell factor;Hippocampal dentate gyrus1497|424|10更新时间：2023-08-11
- 摘要：Objective To observe the effect of"Shuanggu Yitong"(Double-reinforcing and one-unblocking)needling[electroacupuncture(EA)of"Guanyuan"(CV 4),"Housanli"(ST 36),"Fenglong"(ST 40)and"Zhongwan"(CV 12)]on expression of hypothalamic phosphatidylinositol 3-kinase(PI 3K)and p-PI 3Kproteins in insulin resistance rats.Methods Sixty Wistar rats were randomized into normal control,model,EA prevention,EA,cerebrospinal fluid(CSF),and Wortmannin(PI 3K antagonist)groups(n=10rats/group).Insulin resistance model was established by feeding the animal with high fat forage continuously for 8weeks.For rats of the EA group,EA(2Hz,1mA)was applied to ipsilateral"Housanli"(ST 36)-"Fenglong"(ST40),and"Guanyuan"(CV 4)-"Zhongwan"(CV 12)for 15min,once daily,5times a week and for 8weeks,beginning after modeling.For rats of the EA prevention group,EA was conducted simultaneously with the modeling.For rats of the Wortmannin group and CSF group,cerebroventricular microinjection of Wortmannin(50nmol/L,3mg/kg)or artificial CSF was given through an implanted steel cannel.Fasting plasma glucose(FPG)and fasting serum insulin(FINS)contents were assayed using glucosetested instrument and ELISA respectively,insulin activity index(IAI)was calculated[IAI=Ln(1/FPG×FINS)],and the expression of hypothalamic PI 3Kand p-PI 3Kproteins was detected by Western blot.Results In comparison with the normal control group,the body weight(BW),FPG and FINS contents at time-points of 8and 16weeks were considerably increased(P<0.01),and IAI,hypothalamic PI 3Kp 110and p-PI 3Kp 110protein expression levels were obviously decreased in the model group(P<0.01).Compared with the model group,the FPG and FINS contents at the 8th week after modeling in the EA prevention group,the BW and FPG levels at the 16th week in the EA prevention,EA and CSF groups,FINS levels at the 16th week in the EA prevention and EA groups were remarkably decreased(P<0.05,P<0.01),IAI,and hypothalamic PI 3Kp 110 and pPI 3Kp 110protein expression levels were significantly up-regulated in the EA prevention,EA and CSF groups(P<0.05,P<0.01).No significant differences were found among the EA prevention,EA and CSF groups,and between the model and Wortmannin groups in the BW,FPG,FINS,IAI and hypothalamic PI 3Kp 110and p-PI 3Kp 110expression levels(P>0.05).Conclusion EA intervention can reduce insulin resistance by suppressing the increase of body weight,blood glucose and insulin contents,and increasing hypothalamic PI 3Kexpression levels in insulin resistance rats.关键词：Insulin resistance;Electroacupuncture intervention;Fasting plasma glucose;Fasting serum insulin;Hypothalamus;PI 3Kprotein expression1657|340|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture intervention on gastric ulcer(GU)and sleeping quality from the viewpoint of brain-gut axis which plays an important role in the regulation of many vital functions in health and disease.Methods Forty male Wistar rats were randomized into normal control,GU model,acupuncture of"Zhongwan"(CV 12)-"Zusanli"(ST 36,gastric treatment acupoints),acupuncture of"Shenmai"(BL 62)-"Zhaohai"(KI 6,sleep-promotion acupoints),and acupuncture of CV 12-ST 36-BL 62-KI 6(combined treatment)groups,with 8rats in each group.GU model was established by intragastric perfusion of dehydrated alcohol(1mL/rat),and sleep model established by intraperitoneal injection of pentobarbital sodium(40mg/kg)after the last treatment.The abovementioned acupoints were punctured with filiform needles and stimulated by manipulating the needle for about 30s,once every 5min during 20min of needle retention.The treatment was conducted once daily for five days.Gastric mucosal lesion index was assessed by Guth's method,and the mucosal pathological changes were observed under microscope after H.E.staining.The contents of dopamine(DA)in the serum and striatal tissues were detected by ELISA kit.Results Compared with the normal control group,the rats' sleeping duration,and serum DA content were markedly decreased and the gastric mucosal lesion index,and the striatal DA content remarkably increased in the model group(P<0.01).In comparison with the model group,the rats' sleeping duration,and serum DA content were significantly increased,and the gastric mucosal lesion index,and the striatal DA content remarkably down-regulated in the CV 12-ST 36(gastric treatment acupoints),BL 62-KI 6(sleep-promotion acupoints)and CV 12-ST 36-BL 62-KI 6(combined treatment)groups(P<0.05,P<0.01).The effects of the combined treatment group were notably superior to those of the sleep promotion acupoints group in reducing mucosal lesion index and in increasing serum DA level(P<0.01,P<0.05).Conclusion Acupuncture stimulation of"Zhongwan"(CV 12),"Zusanli"(ST 36),"Zhaohai"(KI 6)and"Shenmai"(BL 62)can relieve the gastric mucosal lesion,and prolong the sleeping duration in gastric lesion rats,which may be related to its effects in increasing blood DA and lowering striatal DA level,suggesting a correlation between the gastrointestinal disorders and sleeping.关键词：Gastric mucosal injury;Acupuncture intervention;Sleep duration;Gastric mucosal lesion index;Dopamine;Peripheral blood;Striatum2034|382|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of grain-sized moxibustion at"Xinshu"(BL 15)and"Shenshu"(BL 23)on memory-learning ability and amyloid deposition in transgenic Alzheimer's disease(AD)mice.Methods Seventeen amyloid precursor protein(APP)/presenilin(PS)1(APP+/PS 1+)double transgenic 6799mice aged 3-4weeks were randomly divided into model group(n=9)and moxibustion group(n=8).Nine wide-type(C 57BL/6J)female mice were used as the normal control group.Moxibustion(ignited grain-sized moxa cone)was applied to bilateral"Xinshu"(BL 15)and"Shenshu"(BL 23)for about 30s,once a day for 9courses(10days constitute a therapeutic course,with 2days' break between every two courses).Morris water maze tests were performed to detect the mice's learning-memory ability.The alterations of beta-amyloid deposition(number of the positive plaques)in the cerebral cortex and hippocampus were detected by using an imaging analysis system following Congo red staining of the cerebral tissue sections.Results Compared with the normal group,the average escape latency of place navigation tests was significantly increased(P<0.05),and the target-platform crossing times and percentage of dwell time in the target quadrant of the spatial exploring tests were notably decreased in the model group(P<0.05).After moxibustion intervention,the escape latency was remarkably shortened,and the target-platform crossing times and dwell time percentage were obviously increased in the moxibustion group in comparison with the model group(P<0.05),suggesting an improvement of the learning-memory ability after moxibustion.Results of Congo red staining of the cerebral tissue showed that there were many irregular,uneven staining positive plaques in the cerebral cortex and hippocampus of AD mice in the model group.Compared with the model group,the positive plaque numbers in both cerebral cortex and hippocampus were considerably reduced in the moxibustion group(P<0.05).Conclusion Grain-sized moxibustion at"Xinshu"(BL 15)and"Shenshu"(BL 23)can ameliorate learning-memory ability and restrain the formation of amyloid deposition in AD mice.关键词：Grain-sized moxibustion;APP+/PS 1+transgenic mice;Amyloid deposition;Learning-memory ability;Alzheimer's disease1283|415|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of mild-moxibustion on serum amyloid-A(SAA)protein,cytokine contents and liver amyloid-A protein expression in atherosclerosis rabbits,so as to explore its anti-inflammation mechanism.MethodsA total of 40Japanese male rabbits were equally randomized into normal control,model,mild moxibustion and medication(lovastatin)groups.The atherosclerosis model was established by feeding the animals with high-fat diet for 14weeks and intravenous injection of bovine serum albumin(250mg/kg,once every week,3times altogether).Mild moxibustion was applied to"Shenque"(CV 8),"Housanli"(ST 36)for 10min,once daily for 14weeks beginning from the first day of modeling.The SAA,serum IL-6,TNF-αand IL-10contents were determined by double antibody sandwich method,and liver amyloid-A protein expression was detected by Western blot.The pathological changes of the cervical common artery were detected by H.E.staining.Results In comparison with the normal control group,SAA,serum IL-6,IL-10and TNF-αcontents and liver amyloid-A protein expression level were significantly increased in the model group(P<0.01,P<0.05).Compared to the model group,SAA,serum IL-6and TNF-αcontents,and liver amyloid-A protein expression level were considerably down-regulated and serum IL-10content was obviously up-regulated in the moxibustion and medication groups(P<0.05,P<0.01).No significant differences were found between the moxibustion and medication groups in the five indexes(P>0.05).In addition,the pathological changes of the cervical common arterial endomembrane in the moxibustion and medication group were relatively milder,suggesting aprotective effect of moxibustion on liver tissue in atherosclerosis rabbits.Conclusion Mild moxibustion can relieve liver injury in atherosclerosis rabbits,which is associated with its effects in suppressing serum inflammatory cytokines and liver amyloid-A protein expression and up-regulating anti-inflammatory cytokine IL-10level.关键词：Atherosclerosis;Moxibustion;Liver injury;Amyloid-A protein;Serum inflammatory cytokines;An-ti-inflammatory cytokine1309|507|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupotomy therapy on cervicomuscular apoptosis and apoptosis regulator Bax protein expression in cervical spondylosis(CS)rabbits so as to investigate its mechanisms underlying improvement of CS.Methods Twenty-four New Zealand rabbits were randomly divided into normal control,model,acupotomy and electroacupuncture(EA)groups,with 6rabbits in each group.The CS model was made by forced head-bowing for 5hours in a restrained chamber,once daily for 12weeks.Acupotomy was performed at the starting point of trapezius,the mastoid process attaching point of sternocleidomastoid,the cerverical vertebrae joint process or the local induration or cord-like mass(2or 3points of them were used as the needle-knife entering points),once a week for 3weeks.For animals of the EA group,EA(2Hz/100Hz,2mA)was applied to bilateral"Tianzhu"(BL 10),"Jingbailao"(EX-HN 15),"Dazhu"(BL 11)for 20min,once daily and 3times a week for 3weeks.The number of apoptotic cells in the cervical muscle was observed by light microscope after TUNEL staining and muscular Bcl-2and Bax protein expression was detected by Western blot.Results In comparison with the control group,the number of cervicomuscular apoptotic cells,and the expression level of cervicomuscular Bax protein were significantly increased,and the Bcl-2/Bax was obviously decreased in the model group(P<0.01,P<0.05).Compared to the model group,the number of apoptotic cells and the expression level of muscular Bax protein were notably decreased in the acupotomy group(P<0.01,P<0.05),while the ratio of Bcl-2and Bax was apparently increased in the acupotomy group(P<0.05).The effects of acupotomy were significantly superior to those of EA in lowering apoptotic cell number and in up-regulating Bcl-2/Bax(P<0.01,P<0.05).No significant differences were found between the EA and model groups in the apoptotic cell number and among the four groups in Bcl-2protein expression levels(P>0.05).Conclusion Acupotomy therapy can reduce cervicomuscular cellular apoptosis and Bax protein expression in CS rabbits,which may be one of its mechanism underlying improving CS.关键词：Acupotomy;Cervical spondylosis;Cervical muscle;Apoptosis;Bcl-2protein;Bax protein1291|728|8更新时间：2023-08-11
- 摘要：As an important content of alternative and complementary medicine,acupuncture therapy has been proved to be effective in relieving myocardial ischemia(MI).Authors of the present paper review recent progress of researches on acupuncture therapy in resisting MI from 1)improving cardiovascular function and promoting angiogenesis,and 2)protecting myocardial cells from further injury and reducing cellular apoptosis at different pathological stages of MI.Moreover,the authors discuss the characteristics of epigenetic regulation in the process of MI and cardiac repair including the methylating of DNA,modification of histone,remodeling of the chromatin,and micro-RNA expression,mediating cellular apoptosis,regeneration of myocardial blood vessels,etc.The authors hold that future studies on the underlying mechanisms of acupuncture therapy in the prevention and treatment of MI from epigenetics may be a new approach and a new direction.关键词：Acu-moxibustion therapy;Anti-myocardial ischemia;Epigenetic regulation;Research approach1418|927|14更新时间：2023-08-11
- 摘要：It has been well documented that acupuncture effect is produced through suitable manipulation stimulation of the inserted acupuncture needle,and subsequent activation of the body's inherent nerve-endocrine-immune regulating network.As a physical stimulus,the acupuncture stimulation exerted onto the local acupoint needs being converted into biological signal first,further reaching favorable regulation in the body.Toll-like receptor 4(TLR 4),an important molecular pattern recognition receptor,can not only recognize the exogenous pathogen-associated molecular patterns but also identify endogenous damage-associated molecular patterns,leading to activation of innate immunity.What's more,TLR 4has a close relationship with nervous and immune system of the body.In the present paper,the authors make a discussion on the correlation between TLR 4and acupuncture intervention from 1)TLR 4and acupuncture signal conversion,2)TLR 4and acupuncture clinical phenomenon,and 3)TLR 4and related mechanisms of acupuncture intervention.Moreover,the authors also think that TLR 4in the acupoint area may participate in the initiation process of acupuncture stimulation information.关键词：Toll-like receptor 4;Acupuncture therapy;Acupuncture information initiation;Immuno-regulation1258|370|4更新时间：2023-08-11
- 摘要：Acupuncture therapy has been confirmed to be effective in treating cardiovascular diseases in clinical practice.Acupuncture-induced balance of the autonomic nervous system activities is one of its key mechanisms.In the present paper,the authors review progress of studies on acupuncture treatment of cardiovascular diseases from 1)regulating cardiac sympathetic-beta-adrenergic receptor activity and myocardial intracellular GTP-binding protein(Gs)-adenylylcyclase(AC)-cyclic adenosine monophosphate(cAMP)-protein kinase(PKA)signaling,and 2)balancing cardiac sympathetic and vagal nerve activities.Due to limited experimental conditions,in-depth studies about the mechanisms of acupuncture intervention underlying improvement of cardiovascular functions are relatively fewer up to now.Along with the further development of modern biology,the mechanism of acupuncture intervention underlying regulation of cardiac function via autonomic nerve system will be revealed in detail.关键词：Acupuncture;Autonomic nervous system;Cardiac function;Beta-adrenergic receptors;Cardiovascular disease1161|746|5更新时间：2023-08-11
- Postal code：100700
- Tel：010-64089344,84014607 Email：zcyjbjb@vip.163.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05017590号-8
京公网安备11010802020460号 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0