最新刊期
卷 40 ， 期 1 ， 2015
- 摘要：Objective To observe the effect of electroacupuncture(EA)stimulation of"Housanli"(ST 36)and"Zhongwan"(CV 12)on serum leptin level and expression of phosphorylated Janus kinase 2(p-JAK 2)and phosphorylated signal transducer and activator of transcription 3(p-STAT 3)proteins in the liver tissue of obese rats.Methods Thirty-two male Wistar rats were randomly divided into control group,model group,manual acupuncture(MA)group and EA group(n=8in each group).The obese model was induced by high fat diet for 8weeks.MA or EA(20Hz,1.5V)stimulation was applied to bilateral"Housanli"(ST 36)and"Zhongwan"(CV 12)for 20 min,once daily for 4weeks.The animals' body weight and length were recorded,and Lee's index was calculated.Serum leptin level and liver p-JAK 2and p-STAT 3protein expression levels were detected using radioimmunoassay and Western blot,respectively.Results In comparison with the control group,the rats' body weight,Lee's index,and serum leptin content were remarkably increased in the model group(P<0.05),while hepatocellular p-JAK 2and p-STAT 3protein expression levels were significantly down-regulated in the model group(P<0.05).After treatment for 4weeks,the levels of body weight,Lee's index,serum leptin were significantly reduced(P<0.05),and hepatocellular p-JAK 2and p-STAT 3protein expression in the MA and EA groups were considerably up-regulated compared with the model group(P<0.05).The effect of EA was apparently superior to that of MA in up-regulating p-JAK 2and p-STAT 3protein expression(P<0.05).Conclusion Both EA and MA stimulation can reduce the obese rats' body weight,and Lee's index,which may be closely associated with their effects in down-regulating serum leptin and in enhancing hepatocellular p-JAK 2and p-STAT 3protein expression.关键词：Electroacupuncture;Manual acupuncture intervention;Obese;Serum leptin;Hepatocellular JAK 2/ STAT 3 signaling884|677|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of scalp-acupuncture intervention on the expression of Bcl-2of astrocytes in the hippocampus in vascular dementia(VD)rats,so as to reveal its underlying mechanism in improving VD.Methods A total of40 male SD rats were randomly divided into normal control,sham-operation,VD model,and scalp-acupuncture groups(n=10in each group).The VD model was established by using modified Pulsinelli 4-vessel-occlusion method.Scalp-acupuncture was applied to bilateral"Daozang Shangjiao"and bilateral"Jiyi",and"Siwei"acupoints according to FANG's scalp-acupuncture therapy for 30 min,once daily for 10 days.The rat's learning-memory ability was detected by Morris water maze tests,and the expression of apoptosis related proteins Bcl-2of astrocytes in the hippocampus was detected by immunofluorescence double labeling technique and Western blot,respectively.Results Morris water maze tests showed that the average escape latency of place navigation test and the duration of the first platform-site crossover(space probing test)were significantly increased in the model group compared with the normal control group(P<0.01),and the number of platform-site crossovers in 2min was markedly decreased in the model group(P<0.01).The increased average escape latency and the increased duration of first platform-site crossover were markedly shortened in the scalp-acupuncture group in comparison with the model group(P<0.01),and the number of the platform crossovers in 2min was significantly increased in the scalp-acupuncture group(P<0.01).The expression of Bcl-2protein and the number of GFAP/Bcl-2-labeled cells of hippocampal CA 1region in the model group were remarkably decreased compared with the normal control group(P<0.01).After scalp-acupuncture intervention,the decreased Bcl-2protein expression level and the number of double-labeled GFAP/Bcl-2cells were significantly up-regulated in the scalp-acupuncture group compared with the model group(P<0.01).No significant differences were found between the normal control and sham-operation groups in the escape latency,duration of the first platform-site crossover,number of platform crossovers in 2 min,number of GFAP/Bcl-2double labeled cells and the expression level of hippocampal Bcl-2protein(P>0.05).Conclusion Scalp-acupuncture can effectively regulate the expression of apoptosis related proteins Bcl-2of astrocytes in the CA 1region of hippocampus in rats with VD,which may contribute to its effect in the improvement of VD.关键词：Scalp-acupuncture intervention;Vascular dementia;Learning-memory ability;Hippocampus;CA 1 region;Astrocyte;Bcl-2 protein;GFAP/Bcl-2 double label1150|783|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)intervention on expression of cytochrome P 450 side chain cleavage(P 450scc)and 17β-hydroxysteroid dehydrogenase 3(17β-HSD3)in the testis in partial androgen deficiency of aging male(PADAM)rats so as to reveal its mechanism underlying improving PADAM.Methods Thirty male SD rats were randomly and equally divided into control,model,and EA groups.The PADAM model was established by intraperitoneal injection of cyclophosphamide(20mg·kg-1·d-1),once daily for 5days.EA(20-30 Hz,1-3mA)was applied to bilateral"Shenshu"(BL 23)and"Guanyuan"(CV 4)for 15 min,once daily for 8weeks.Serum total testosterone(TT)and free testosterone(FT)levels were measured using enzyme-linked immunosorbent assay(ELISA).The expression levels of P 450scc/17β-HSD3 proteins and mRNA in the testis tissue were assayed by immunohistochemistry,Western bolt(WB)and RT-polymerase chain reaction(RTPCR),separately.Results Compared with the control group,both serum TT,FT levels and expression levels of P 450scc/17β-HSD3 proteins and mRNA in the testis tissue in the model group were significantly down-regulated(P<0.01).After EA intervention,compared with the model group,the cyclophosphamide-induced decrease of serum TT,FT levels and the expression levels of P 450scc/17β-HSD3 proteins and mRNA in the testis was reversed in the EA group(P<0.01).Conclusion EA intervention is effective in up-regulating serum TT and FT,testicular P 450 scc and 17β-HSD3 proteins and mRNA levels in PADAM rats,which may be one of its mechanisms underlying improvement of PADAM.关键词：Partial androgen deficiency of aging male;Electroacupuncture intervention;Testis;Cytochrome P 450 side chain cleavage;17 β-hydroxysteroid dehydrogenase 31077|237|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of Zhuang-medical thread moxibustion combined with needle-pricking on vascular oxidative stress injury in oxidative stress injury rats.Methods Eighty Wistar rats were randomly allocated to normal control,sham operation(sham),model,and combined treatment groups(n=20in each group).The oxidative stress injury model was established by ligation of the left sciatic nerve to induce chronic constriction injury(CCI)pain stress stimulation.Zhuangmedical thread moxibustion was applied to bilateral"Zusanli"(ST 36),once a day for 3weeks.Needle-pricking was applied to left"Yanglingquan"(GB 34)and left"Huantiao"(GB 30),once a day for 3weeks except Sundays.Plasma 6-keto-PGF 1α,thromboxane B 2(TXB 2),NO and ET contents were assayed by radioimmunoassay.COX-2immunoactivity of the femoral artery was determined by immunohistochemistry,and pathological changes of the femoral artery were detected by H.E.staining.Results Compared with the control group,the levels of plasma 6-keto-PGF 1αand NO in the model group were significantly reduced(P<0.05),while those of plasma TXB 2and ET and COX-2expression in the femoral artery were obviously increased in the model group(P<0.01).After moxibustion plus needle-pricking treatment,CCI-induced decrease of plasma 6-keto-PGF 1αand NO contents,and increase of plasma TXB 2and ET and COX-2expression levels were obviously reversed(P<0.05,P<0.01).The tubal wall of the femoral artery in rats of the model group got thicker,while that of the combined treatment group was relatively thinner,suggesting an inhibition of vascular intimal hyperplasia after the treatment.Conclusion Zhuang-medical thread moxibustion combined with needle-pricking of ST 36,GB 34 and GB 30 can reduce the expression of femoral artery COX-2and regulate the balance of both plasma PGI 2/TXA 2and plasma NO/ET in CCI-induced oxidative stress rats,which may contribute to its effect in suppressing oxidative stress-induced vascular intimal hyperplasia.关键词：Zhuang-medical thread moxibustion;Needle-pricking;Chronic compressive injury of the sciatic nerve;Antioxidation;Vessel intimal hyperplasia865|258|2更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)of"Dazhui"(GV 14),"Baihui"(GV 20),etc.on learning-memory ability and expression of vascular endothelial growth factor(VEGF)mRNA,VEGF receptor 1(VEGFR-1/Flt-1)mRNA,and VEGFR-2(Flk-1)mRNA in the hippocampus in vascular cognitive impairment(VCI)rats so as to reveal its mechanism underlying improvement of VCI.Methods A total of 60 Wistar rats were randomly divided into sham operation,VCI model,EA and medication groups(n=12in each group).The VCI model was established by occlusion of the bilateral vertebral arteries and bilateral cervical arteries.EA(2Hz/20Hz)was applied to"Baihui"(GV 20),"Dazhui"(GV 14),"Shuigou"(GV 26)and"Shenting"(GV 24)for 20 min,once daily for 20 days.Rats of the medication group were treated by intragastric perfusion of Aniracetam capsules(0.0625g/kg),once daily for 20 days.The rats' learning-memory ability was detected by step-down test.The expression levels of VEGF mRNA,Flt-1mRNA and Flk-1mRNA in the hippocampus were detected by RT-PCR,and the neurological deficit scores were assessed by Zea Longa(0-4scaling)method.Results Compared with the sham operation group,rats of the model group showed a significant increase in reaction time and error number and decrease of escape latency(reduction of learning-memory ability),and increase in neurological deficit score,and in expression levels of hippocampal VEGF mRNA,Flt-1mRNA and Flk-1mRNA(all P<0.01).After EA treatment,in comparison with the model group,the learning-memory ability and hippocampal VEGF mRNA,Flt-1mRNA and Flk-1mRNA were apparently increased,neurological deficit score were markedly decreased in the EA group(all P<0.01).The effects of EA treatment was obviously superior to those of medication in raising learning-memory ability and up-regulating hippocampal VEGF mRNA and Flt-1mRNA expression levels,and in reducing neurological deficit score(all P<0.05).Conclusion EA intervention can apparently up-regulate hippocampal VEGF mRNA,Flt-1mRNA and Flk-1mRNA expression in VCI rats,which may contribute to its effect in improving learning-memory ability,possibly by promoting neovasculization.关键词：Vascular cognitive impairment;Electroacupuncture treatment;Learning-memory ability;Hippocampus;Vascular endothelial growth factor mRNA982|681|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)treatment on the level of hippocampal amyloidbeta peptide(Aβ)and its key transport receptor low density lipoprotein receptor-related protein-1(LRP 1)in APP/PS 1transgenic mice so as to explore its mechanism underlying improvement of Alzheimer's disease(AD).Methods Twenty-four male APP/PS 1transgenic mice were equally and randomly divided into model group and EA treatment group,and 12C57BL/6mice were used as the normal control group.EA(1Hz/50 Hz,0.3mA)was applied to"Baihui"(GV 20)and"Yongquan"(KI 1)for15min,once every other day for 6weeks.The learning-memory ability was detected by using Morris water maze testing,left hippocampal Aβ1-40 and Aβ1-42 contents were assayed by ELISA,and right hippocampal LRP 1expression was detected using Western blot(WB).Results Results of Morris water maze test showed no significant differences among the three groups in the escape latency,the times of the platform-site crossovers,the time spent in the target platform quadrant(P>0.05).Compared with the model group,the moderately increased escape latency had a decreasing tendency in the EA treatment group.ELISA assaying showed that hippocampal Aβ1-42,Aβ1-40,and ratio of Aβ1-42/Aβ1-40 of the model group were significantly higher than those of the normal control group(P<0.01).After EA intervention,the increased Aβ1-42,Aβ1-40,and ratio of Aβ1-42/Aβ1-40 were remarkably down-regulated in the EA treatment group(P<0.01).WB detection displayed that the right hippocampal LRP 1expression level of the model group was markedly lower than that of the normal control group(P<0.05).After EA treatment,LRP 1expression level was moderately up-regulated but without significant difference between the model and EA treatment groups(P>0.05).Conclusion EA intervention can lower the level of hippocampal Aβin APP/PS 1transgenic mice,but its effects on Aβtransport receptor LRP 1expression and learning-memory ability need being confirmed further.关键词：Electroacupuncture;Alzheimer's disease;Amyloidβ;Low density lipoprotein receptor-related protein-1;Hippocampus845|721|11更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)stimulation of"Xiaohai"(SI 8)and"Xiajuxu"(ST 39)on serum TNF-αand duodenal high mobility group protein B 1(HMGB 1)levels in rats with duodenal ulcer(DU),so as to analyze its underlying mechanism in improving DU.Methods Forty SD rats were randomly divided into normal control,model,Xiaohai(SI 8)and Xiajuxu(ST 39)groups(n=10in each group).The DU model was established by intramuscular injection of10% Cysteamine hydrochloride(300mg/kg),followed by taking water containing 1% Cysteamine hydrochloride for 48 h.EA(10Hz/50 Hz,1-3mA)was applied to bilateral SI 8and ST 39 for 30min,once a day for 10 days.The DU score(0-5score)was evaluated according to Moraes' method.Serum TNF-αcontent was determined by ELISA and duodenal HMGB 1expression was measured by immunohistochemistry.Results Compared to the normal control group,the DU score,serum TNF-αcontent,and duodenal HMGB 1expression level of the model group were significantly higher(P<0.01).Following EA intervention,the increased DU score,serum TNF-αcontent,and duodenal HMGB 1expression level were considerably down-regulated in the two EA groups in comparison with the model group(P<0.01,P<0.05)except HMGB 1in the SI 8group(P>0.05),and the effect of EA of"Xiajuxu"(ST 39),the lower-He point,was remarkably superior to that of"Xiaohai"(SI 8)in reducing DU score and TNF-αcontent(P<0.01).Conclusion EA stimulation of SI 8and ST 39 can improve duodenal ulceration in DU rats,which may be related to its effect in down-regulating serum TNF-αand duodenal HMGB 1expression.The therapeutic effect of ST 39 is markedly better than SI 8in relieving duodenal ulceration.关键词：Electroacupuncture;Duodenal ulceration;DU score;Serum TNF-α;High mobility group protein B 1;Acupoint-action specificity903|368|1更新时间：2023-08-11
- 摘要：Objective To observe the impact of preconditioning of EA stimulation of"Baihui"(GV 20)and"Shuigou"(GV 26)in different duration on expression of matrix metalloproteinase(MMP)-9and vascular endothelial growth factor(VEGF)in blood-brain barrier(BBB)tissues in rats with cerebral ischemia-reperfusion injury(CI/RI).Methods Sixty-four male SD rats were randomly allocated to sham-operation(sham),model,EA-pretreatment 7days(pre-t-7d)and EA-pre-t-15dgroups(16rats/group,8rats for immunohistochemistry and 8for RT-PCR).The CI/RI model was established by occlusion of the middle cerebral artery for 90 min and reperfusion.EA(2Hz/15 Hz,1mA)was applied to GV 20 and GV 26 for 30min,once daily for 7days and 15 days respectively before modeling.Immunohistochemical staining and fluorescence quantitative RT-PCR were employed to detect the expression of MMP-9protein,MMP-9mRNA and VEGF mRNA in the BBB tissues(choroid plexus).Results Compared with the sham group,the number of MMP-9immuno-reaction positive cells,and the expression of MMP-9mRNA and VEGF mRNA in the model group were significantly increased(P<0.001),while compared with the model group,the number of MMP-9positive cells,and expression levels of MMP-9mRNA and VEGF mRNA in the EA-pre-t-7dand EA-pre-t-15 dgroups were significantly reduced(P<0.01),and the effects of EA-pre-t-15 dwere markedly superior to those of EA-pre-t-7d(P<0.01,P<0.05).Conclusion EA-pretreatment of GV 20 and GV 26 can effectively restrain CI/RI-induced increase of expression of MMP-9protein,MMP-9mRNA and VEGF mRNA in blood-brain barrier in CI/RI rats,which may contribute to its protective effect of ischemic cerebral tissue.Longer EA-pretreatment is relatively better.关键词：EA-pretreatment;Cerebral ischemia-reperfusion;Blood-brain barrier;Matrix metalloproteinase(MMP)-9;Vascular endothelial growth factor961|868|16更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)stimulation of"Neiguan"(PC 6)on expression of sodium(Nav)channelα-subunit 1.5and Navβ-subunitsβ1—β4(the known myocardial sodium channel proteins)in acute myocardial ischemia(AMI)rats so as to explore its mechanisms underlying protection of ischemic myocardium.Methods Sixty SD male rats were randomly divided into normal control,AMI model,Neiguan(PC 6),Lieque(LU 7)and non-acupoint groups.The AMI model was established by intravenous injection of Isoprenaline(85mg/kg)once daily for 2days.Myocardial Nav1.5and Navβ1,β2,β3,β4protein expression levels were detected by Western blot.Results In comparison with the control group,myocardial Nav 1.5,β1,β2,β3andβ4protein expression levels were significantly down-regulated in the AMI model group(P<0.01).After EA stimulation,compared with the model group,the expression levels of Nav 1.5,Navβ1,β2,β3andβ4protein expression levels were significantly up-regulated in the Neiguang(PC 6)and Lieque(LU 7)groups(P<0.01)rather than in the non-acupoint group(P>0.05).There was no statistical significance between the Neiguan(PC 6)and Lieque(LU 7)groups inβ4protein expression level(P>0.05).Conclusion EA stimulation of both PC 6and LU 7can significantly reverse AMI-induced down-regulation of myocardial Nav 1.5,β1,β2,β3andβ4protein expression levels in AMI rats,which might contribute to its function in improving AMI by reducing calcium overload.关键词：Myocardial ischemia;Electroacupuncture;Neiguan(PC 6);Voltage-gated sodium channel947|542|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture stimulation of Ashi-point on hepatocyte growth factor(HGF)protein expression in gastrocnemius muscle(GM)in rats with traumatic injury of GM,so as to reveal its mechanism underlying improvement of traumatic injury of skeletal muscle.Methods A total of 80 SD rats were randomly divided into normal control(n=8),model,acupuncture control,and acupuncture treatment groups,and the latter 3groups were further allocated to 0h,24 h and 48hsubgroups(n=8in each subgroup).The acute soft tissue contusion model was established by using 500g-counterweight free falling hitting at the GM of the right leg(height 30cm).The Ashi-point was punctured with a piece of filiform needle by penetrating the site beside the injured GM,and stimulated for about 5seconds.For acupuncture control group,acupuncture was performed in the same Ashi-point in normal rats.Pathological changes of the focal GM were observed by H.E.staining and HGF protein expression of GM was detected by Western blot.Results H.E.staining showed that different degrees of injury of GM tissue including broken muscle fibers with cellular swelling,inflammatory cell infiltration,muscle fibrinolysis,atrophy,degeneration and necrosis,etc.,were found at each time-point after modeling,while in the acupuncture treatment group,the severity of muscle injury was relatively milder particularly at the time-points of 24 hand 48h.Compared with the normal control group,HGF protein expression levels of GM at the 3time-points in the model group,and at 48 hin the acupuncture control group were markedly increased(P<0.05,P<0.01);while in comparison with the model group,HGF protein expression levels were remarkably downregulated at 24 hand 48hafter acupuncture treatment(P<0.01,P<0.05).Conclusion Acupuncture stimulation of Ashi-point can significantly down-regulate HGF protein expression in the GM in soft tissue contusion rats,which may contribute to its effects in relieving contusion injury of skeletal muscle.关键词：Soft tissue contusion;Acupuncture intervention;Hepatocyte growth factor;Gastrocnemius muscle1010|349|6更新时间：2023-08-11
- 摘要：Objective To investigate short-and long-term benefits and safety of acupoint injection of kakkonein for early-or mid-stage Parkinson's disease(PD).Methods A total of 135 patients with early-or mid-stage PD were enlisted and 116 cases completed the observation,including 40 cases in the acupoint-injection group,37 cases in the acupuncture group and 39 cases in the medication group.Patients of the acupoint-injection group were treated by injection of kakkonein(2mL)into bilateral Fengchi(GB 20),once very other day for 8weeks.For acupuncture intervention,bilateral GB 20 acupoints were punctured with filiform needles,once every other day for 8weeks.Patients of the medication group were treated by oral administration of compound levodopa(125mg/time,twice a day)for 8weeks.The disease severity was assessed by using Unified Parkinson's Disease Rating Scale(UPDRS,version 3.0)before and after treatment,and one month after the treatment.Results After the treatment,scores of psychosis,behavior and emotion,daily life activity and motor function were all significantly decreased in the three groups in comparison with pre-treatment in each group(P<0.05),suggesting an improvement of the PD patients' conditions.The therapeutic effect of acupoint-injection and acupuncture groups was markedly superior to that of the medication group(P<0.05).One-month's follow-up showed that the scores of behavior-emotion and daily life activity were obviously lower in the acupoint-injection group than in the acupuncture and medication groups(P<0.05).No significant differences were found between the acupuncture and medication groups in down-regulating scores of behavior-emotion,daily life activity and motor function(P>0.05).Conclusion Acupoint injection of kakkonein can improve patients' behavior,emotion,and daily life activity in early-or mid-stage PD patients and has both short-and longer-term benefits.关键词：Parkinson's disease;Acupoint injection;Kakkonein;Multicenter randomized controlled trial1025|708|1更新时间：2023-08-11
- 摘要：Objective To observe the clinical effect of acupuncture therapy combined with Lipitor in the treatment of primary hyperlipidemia(spleen deficiency and food stagnation type).Methods Sixty primary hyperlipidemia patients(spleen deficiency and food stagnation type)were equally randomized into medication group and acupuncture plus medication group.Patients of both groups were treated by oral administration of Lipitor(20mg/tablet,one tablet per day)for 6weeks.Manual acupuncture stimulation was applied to Baihui(GV 20),Zhongwan(CV 12),and bilateral Zusanli(ST 36),Sanyinjiao(SP 6),Yinlingquan(SP 9)and Fenglong(ST 40)for 40 min,twice daily for 6weeks except the weekends.Serum total cholesterol(TC),triglyceride(TG),high density lipoprotein-cholesterol(HDL-C),low density lipoprotein-cholesterol(LDL-C)were detected by using a full-automatic biochemistry analyzer before and after the treatment.The therapeutic effect was assessed according to the standards described in"Guide Principles for Clinical Researches of New Chinese Herbal Drugs".Results After the treatment,the levels of serum TC,TG of the two groups were significantly decreased in comparison with pre-treatment in the same one group(P<0.05),and the therapeutic effect of the acupuncture+medication group was significantly superior to that of simple medication group in lowering serum TC,TG and LDL-C levels and heightening HDL-C levels(P<0.05).Of the two 30 cases in the medication and acupuncture+medication groups,2(6.7%)and 7(23.3%)were basically controlled in their blood-lipid levels,10(33.3%)and 15(50.0%)had a marked improvement,10(33.3%)and 6(20.0%)were improved,and 8(26.7%)and 2(6.7%)were invalid,with the effective rates being 73.3% and 93.3%,respectively.Conclusion Acupuncture combined with administration of Lipitor is effective in improving primary hyperlipidemia in patients,which is superior to administration of simple Lipitor.关键词：Primary hyperlipidemia;Acupuncture therapy;Lipitor;Blood-lipid852|250|1更新时间：2023-08-11
- 摘要：Objective To observe the curative effect of acupoint-injection and intramuscular-injection of Bacillus Calmette-Guerin(BCG)Polysaccharide Nucleic Acid for bronchial asthma.Methods Sixty patients with bronchial asthma were equally randomized into acupoint-injection group and intramuscular-injection group.For patients of the acupoint-injection group,2mL of BCG was injected into bilateral Feishu(BL 13,1mL for one side)once per day in the first 15 days,and once every other day in the rest 2.5months except weekends.Intramuscular-injection was conducted at the lateral sites of the left or right buttock,2mL/time for each site,and the injection frequency was the same to that of the acupoint-injection.The therapeutic effect was assessed according to the standards of Guide Principles for Clinical Research of New Chinese Herbal Drugs(2002)and Asthma Group of Breathing Diseases of China Medical Association(2008).Changes of FEV 1(forced expiratory volume in the first second)/FVC(forced vital capacity)and PEF%(peak expiratory flow)were detected using apulmonary function detector.Serum IgA,IgM,IgG and IgE contents were assayed by using an autonomic biochemical analyzer.Results After 3months' treatment,the scores of patients' symptoms and signs and serum IgE and IgG levels were significantly decreased in both muscular-injection and acupoint-injection groups(P<0.01),while asthma controlled test(ACT)scores FEV 1/FVC% and PEF% values were considerably increased in both groups compared to pre-treatment in the same one group(P<0.01).The effects of acupoint-injection were markedly superior to those of the intramuscular-injection in reducing clinical symptom-sign score,and serum IgE content,and in up-regulating ACT score,FEV 1/FVC% and PEF%levels(P<0.05,P<0.01).There were no significant differences in serum IgG,IgA and IgM levels between the two groups 3months following the treatment(P>0.05).Conclusion Acupoint-injection of BCG polysaccharide nucleic acid can effectively improve bronchial asthma patients' clinical symptoms and sings and pulmonary function,which may be closely associated with its function in down-regulating serum IgE and IgG levels.关键词：Bronchial asthma;Acupoint-injection;Intramuscular-injection;Bacillus Calmette-Guerin(BCG)Polysaccharide Nucleic Acid;Serum immunoglobulin;Pulmonary function869|488|1更新时间：2023-08-11
- 摘要：Objective To observe the therapeutic effect of body acupuncture combined with auricular acupressure for treatment of menstrual headache of hyperactivity of"liver fire".Methods A total of 85 menstrual headache patients with hyperactivity of"liver-fire"were randomly divided into control group(n=42)and treatment group(n=43).Patients of the control group were treated by oral administration of Flunarizine Hydrochloride Capsules(Sibelium,5mg/time,twice daily)for 5days beginning at the headache attack in the first menstrual cycle,and for two weeks(except weekends)in the second and third menstrual cycles.Patients of the treatment group were treated by manual acupuncture stimulation of body acupoints Baihui(GV 20),Fengchi(GB 20),Sanyinjiao(SP 6),Taichong(LR 3),Xiaxi(GB 43),Taiyang(EX-HN 5),Hanyan(GB 4),Xuanlu(GB 5)and Shuaigu(GB 8)beginning from the headache attack,and otopoint-pellet pressure of otopoints unilateral Endocrine,Ovaries,Shenmen,Cortex,Liver,Spleen and Kidney(beginning 5days before menstrual onset)and bilateral Shenmen,Nie,Liver,Gallbladder,Subcortex and Jiaogan during headache attack.Clinical symptom scores were assessed according to the"Guiding Principles for Clinical Research on New Chinese Herbal Drugs"issued by the Chinese Ministry of Health.Results After the treatment,of the 42 and 43cases in the control and treatment groups,9(21.43%)and 20(46.51%)were cured,12(28.57%)and 14(32.56%)had a marked improvement,13(30.95%)and 7(16.28%)were effective,8(19.05%)and 2(4.65%)invalid,with the effective rates being 80.95% and 95.35%,respectively.The curative effect of the treatment group was significantly better than that of the control group(P<0.05).The symptom score and VAS score after the treatment and 3months post-treatment in the treatment group were significantly lower than those of the control group(P< 0.01).The headache scores and VAS scores were significantly reduced following the treatment and 3 months after the treatment in both control and treatment groups(P<0.01).After the treatment for 2-3therapeutic courses,the headache attack duration during menstruation was markedly and gradually shortened in both groups(P<0.01),and therapeutic effect of the treatment group was significantly better than that of the control group(P<0.01).Conclusion Body acupuncture combined with auricular acupressure treatment can effectively relieve menstrual headache in menstrual women with hyperactivity of"liver fire".关键词：Body acupuncture;Auricular acupressure;Menstrual headache;Visual Analogue Scale;Randomized controlled clinical trial952|688|0更新时间：2023-08-11
- 摘要：A search on acupuncture Deqi was conducted using four Chinese-language biomedical databases(CNKI,WanFang,VIP and CBM)and PubMed database and using keywords"Deqi"or"needle sensation""needling feeling""needle feel""obtaining qi",etc.Then,a "Modern Literature Database for Acupuncture Deqi"was established by employing Microsoft SQL Server 2005 Express Edition,introducing the contents,data types,information structure and logic constraint of the system table fields.From this Database,detailed inquiries about general information of clinical trials,acupuncturists' experience,ancient medical works,comprehensive literature,etc.can be obtained.The present databank lays a foundation for subsequent evaluation of literature quality about Deqi and data mining of undetected Deqi knowledge.关键词：Acupuncture Deqi;Literature classification;SQL Server Database;Literature information inquiry1038|463|4更新时间：2023-08-11
- 摘要：The exosome,released from mast cells,T cells,B cells and many other types of cells,is the common form of vesicle transportation between cells and participates in the exchange of information between cells,and may be also involved in acupuncture induced clinical effects.In the present paper,the authors reviewed recent development of researches on this field from 1)acupuncture stimulation induces changes of number and function of mast cells in the local acupoint area,probably being the key factor for initiating acupuncture effect;2)acupuncture stimulation induces release of neurotransmitters,hormones,cytokines,Ca2+,etc.,in the local acupoint region,possibly being closely associated with the production of clinical effects;3)acupuncture stimulation results in excitation of sensory afferent nerve fibers,triggering neuro-regulation;4)exosomes derived from mast cells contain multiple neurotransmitters,hormones,cytokines,etc.to activate immune cells and sensory afferent fibers,inducing immuno-regulation and neuro-regulation;and 5)acupuncture stimulation induced release of Ca2+,ATP,etc.may potentiate release and transportation of exosomes.However,current researches are lack of excavation of network connection and transformation from basic research to clinical application.The authors hold that the exosome,released from mast cells by needling acupoints,acts as a messenger in network connection of nerve-mast cell-signal molecule in the body and may be one of the key factors of therapeutic effects.关键词：Mast cell;Exosome;Acupuncture effect;Initiating factor1156|860|3更新时间：2023-08-11
- Postal code：100700
- Tel：010-64089344,84014607 Email：zcyjbjb@vip.163.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05017590号-8
京公网安备11010802020460号 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0