最新刊期
卷 40 ， 期 2 ， 2015
- 摘要：Objective To observe the effect of electroacupuncture of"Guanyuan"(CV 4)on bone miner density,bone biomechanics,serum osteocalcin(BGP)and alkaline phosphatase(ALP)contents,and femoral osteoblastic Wnt-β-catenin signaling in postmenopausal osteoporosis(PMOP)rats.Methods Forty female SD rats were randomly divided into sham operation(sham),model,EA-CV 4,and EA-non-acupoint(below the costal region)groups(n=10in each group).The PMOP model was established by performing an ovariectomy in the rats of the later 3groups.EA(2Hz,1mA)was applied to CV 4for 20 min,once daily for one month,with one day's break between every 10 days.After the treatment,serum BGP and ALP contents were detected using ELISA,the right femoral bone miner density and biomechanics(maximum load,breakage load)were measured using a Dual Energy X-Ray Bone Densitometer and a Universal Material Testing Instrument,respectively.The expression levels of Wnt 3a mRNA,β-catenin mRNA and the bone-specific factors runt-related transcription factor 2(Runx 2)mRNA of the femoral bone tissue were determined by real time RT-PCR.Results HE staining results suggested EA of"Guanyuan"(CV 4)can improve the morphological changes(trabeculae)of osteoporosis in ovariectomized rats.Compared with the control group,femoral maximum load and breakage load,bone density and serum BGP and ALP contents,femoral Wnt 3amRNA,β-catenin and Runx 2mRNA expression levels,and femoral Wnt 3aandβ-catenin immunoactivity were significantly down-regulated in the ovariectomized rats(model group)(P<0.05).Following EA treatment,all the decreased levels of femoral maximum load and breakage load,bone density and serum BGP and ALP,femoral Wnt 3amRNA and protein,β-catenin mRNA and protein and Runx 2mRNA expression were obviously reversed in the EA-CV 4group(P<0.05)rather than in the non-acupoint group(P>0.05).Conclusion EA of CV 4can improve the femoral biomechanics,increase bone density in OVX rats,which are associated to its effects in up-regulating levels of serum BGP and ALP,femoral Wnt 3amRNA and protein,β-catenin mRNA and protein and Runx 2mRNA expression,suggesting an involment of improved bone metabolism and activation of osteoblastic Wnt-β-catenin signaling.关键词：Postmenopausal osteoporosis;Electroacupuncture intervention;Bone miner density;Bone biomecha-nics;Serum osteocalcin;Alkaline phosphatase;Wnt-β-catenin signaling1035|740|9更新时间：2023-08-11
- 摘要：Objective To observe the effect of Electroacupuncture(EA)stimulation of"Tianquan"(PC 2),"Quze"(PC 3),"Neiguan"(PC 6),"Daling"(PC 7)of the Pericardium Meridian on cerebral angiogenesis in cerebral ischemia(CI)rats,so as to reveal its mechanisms underlying improvement of stroke.Methods A total of 50 SD rats were equally randomized into normal control,sham,model,EA-Pericardium-Meridian acupoints(EA-PCM)and EA-Lung-Meridian acupoint(EA-LUM)groups.The CI model was established by occlusion of the middle cerebral artery.EA(2-4V,20Hz)was applied to PC 2,PC 3,PC 6,PC 7and"Tianfu"(LU 3),"Chize"(LU 5),"Lieque"(LU 7),"Taiyuan"(LU 9)of the Lung Meridian for 30 min,once at timepoints of 0h,6h,24 h,48hand 72 h,respectively after modeling.Serum nerve growth factor(NGF)and Nogo protein-A(Nogo-A)contents were assayed by enzyme linked immunosorbent assay(ELISA),and cerebral NGF and Nogo-A immunoactivity levels in the ischemic cerebral tissue were detected by immunohistochemistry.Results(1)Compared to the normal control group,serum NGF and Nogo-A contents,and cerebral NGF immunoactivity level in the model group were significantly increased(P<0.01).Following EA interventions,serum and cerebral NGF levels were further significantly up-regulated in the EA-PCM and EA-LUM groups(P<0.01),while serum Nogo-A contents were down-regulated in the two EA groups(P<0.01).The effect of EA-PCM was markedly superior to that of EA-LUM in up-regulating serum and cerebral NGF levels and down-regulating serum Nogo-A level(P<0.01).No significant differences were found between the normal control and sham groups in serum and cerebral NGF and Nogo-A levels(P>0.05),and among the 5groups in cerebral Nogo-A levels(P>0.05).Conclusion EA stimulation of acupoints of both Pericardium Meridian and Lung Meridian can up-regulate serum NGF,cerebral NGF expression and downregulate serum Nogo-A in CI rats,and the effect of Pericardium Meridian is markedly superior to that of Lung Meridian,suggesting apossible better nerve repair effect of EA-PCM acupoints on ischemic brain.关键词：Cerebral ischemia;Electroacupuncture;Acupoints of Pericardial Meridian;Acupoints of Lung Meri-dian;Nerve growth factor;Nogo protein-A;Nerve repair933|567|14更新时间：2023-08-11
- 摘要：Objective The present study aimed at observing the effect of moxibustion pretreatment on the expression of cerebral microRNAs and Aquaporin protein-4(AQP 4)in rats with cerebral ischemia and reperfusion(CI/R),so as to reveal its mechanism underlying improvement of cerebral infarction.Methods A total of 130 Wistar rats were randomly divided into blank control(n=10),CI/R model(n=30),electroacupuncture(EA,n=30),moxibustion(n=30),Aspirin groups(n=30).Before modeling,EA(2Hz/5Hz,1-2mA)or moxibustion was applied to"Baihui"(GV 20),"Fengfu"(GV 16)and"Dazhui"(GV 14)for 20 min,once daily for 7days.The rats of the Asprin group were treated by intragastric administration of Aspirin(10mg/kg,1mg/mL),once daily for 7days before modeling.The CI/R model was established by occlusion of the bilateral carotid arteries.The expression levels of cerebral miRNAs and AQP 4were detected by real-time PCR and Western blot,respectively.ResultsCompared with the blank control group,the expression levels of cerebral miRNA 290 and miRNA 494 were significantly reduced,while that of AQP 4was obviously up-regulated in the model group(P<0.01).After pretreatment with EA and moxibustion,the relative expression levels of miRNA 290 and miRNA 494 were significantly higher in the EA,moxibustion and Aspirin pretreatment groups than in the model group(P<0.01),while cortical AQP 4expression levels were significantly lower in the EA,moxibustion and Aspirin pretreatment groups than in the model group(P<0.01,P<0.05).The effects of both EA and moxibustion groups were significantly superior to those of Aspirin pretreatment group in up-regulating expression of miRNA 290 and miRNA 494 and down-regulating expression of AQP 4(P<0.01,P<0.05).In addition,the EA pretreatment was markedly superior to moxibustion pretreatment in the aforementioned effects(P<0.05).Conclusion EA pretreatment of GV 14,GV 16 and GV 20 can effectively up-regulate cerebral cortical miRNA 290 and miRNA 494 and down-regulate AQP 4in CI/R rats,which may contribute to its effect in preventing the cerebral tissue from ischemia/reperfusion injury.1126|958|13更新时间：2023-08-11
- 摘要：Objective To observe the effect of Zhuang-medicine medicated-thread moxibustion therapy on interstitial cells of Cajal(ICC)in the gastric antrum of diabetic gastroparesis(DGP)rats,so as to investigate its mechanism underlying improving DGP.Methods Sprague-Dawley(SD)male rats were randomly divided into 3groups:control(n=30),model(n=30)and moxibustion(n=30).The DGP model was established by intraperitoneal injection of streptozotocin(STZ)and by feeding the rats with high fat-sugar forage.Zhuang-medicine medicated-thread moxibustion was applied to"Zhongwan"(CV 12),bilateral"Pishu"(BL 20),"Weishu"(BL 21),"Neiguan"(PC 6)and "Zusanli"(ST 36)once per day,for 3weeks except weekends.The gastrointestinal propulsion rate and weight of stool in 24 hwere determined,and c-kit(a marker for ICC)expression of the gastric antrum tissue was measured by immunohistochemistry.Results The stool weight was significantly higher in the model group than in the control group(P<0.01),but the rate of gastrointestinal propulsion and the rate of c-kit immunoreaction(IR)positive cells in the gastric antrum tissue were significantly lower in the model group than in the control group(P<0.01).After moxibustion,the increased stool weight and the decreased gastrointestinal propulsion rate and decreased c-kit IR-positive cell rate were reversed(P<0.01).Conclusion Zhuang-medicine medicated-thread moxibustion therapy can improve gastrointestinal function in DGP rats,which may be associated with its effect in up-regulating the expression of c-kit IR-positive ICC.关键词：Zhuang-medicine medicated-thread moxibustion;Diabetic gastroparesis;Gastrointestinal propulsion rate;Interstitial cells of Cajal;Gastric antrum;c-kit protein917|385|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA),Polysaccharide of Gastrodia elata Blume(PGB),and EA+PGB on the expression of Nestin and stem cell factor(SCF)in the frontal lobe cortex around the ischemic loci of cerebral ischemia(CI)rats,so as to explore its mechanisms underlying improvement of CI.Methods A total of 40 SpragueDawley adult rats were randomly divided into normal control,CI model,EA intervention,PGB intervention and EA+PGB groups(n=8in each group).The CI model was prepared by middle cerebral artery occlusion.EA(2Hz,2V)was applied to"Baihui"(GV 20)and left"Zusanli"(ST 36)for 30 min,once daily for 14 days.Rats of the PGB and EA+PGB groups were treated by gastrogavage of PGB at a dose of 100mg/kg,once daily for 14 successive days.The expression of Nestin and SCF in the frontal lobe around the ischemic loci of the frontal lobe was detected by immunohistochemistry.Results Compared with the normal control group,the expression levels of regional cerebral cortical Nestin and SCF proteins were significantly increased in the model group(P<0.05).After the treatment,the expression levels of Nestin and SCF were significantly further up-regulated in the EA,PGB and EA+PGB groups in comparison with the model group(P<0.05).Conclusion EA combined with PGB can significantly up-regulate the expression of Nestin and SCF in the frontal lobe around the ischemic loci in cerebral ischemia rats,which may contribute to their function in improving CI.关键词：Electroacupuncture;Cerebral ischemia;Polysaccharide of Gastrodia elata Blume;Nestin;Stem cell factor936|332|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)preconditioning on the expression of neuronal nitric oxide synthase(nNOS),inducible nitric synthase(iNOS)and glial fibrilliary acidic protein(GFAP)in the cortex of focal cerebral ischemia-reperfusion(CI/R)rats so as to explore its underlying mechanism in the protection of ischemic cerebral tissue.Methods Twenty-four Sprague-Dawley rats were randomized into sham operation(sham),model,and EA preconditioning groups(n=8in each group).The CI/R model was induced by intraluminal middle cerebral artery occlusion(MCAO)with a nylon monofilament suture.Before modeling,EA(2Hz/15 Hz,3V)was applied to"Baihui"(GV 20)and"Dazhui"(GV 14)for 30 min,once daily for 7consecutive days.The neurologic impairment score was assessed by using Longa standards and the survival number of neurons in the local ischemic cerebral cortex was determined after Nissl staining,and the expression of nNOS,iNOS and GFAP in the cerebral cortex was detected using immunohistochemistry.Results Compared with the sham operation group,the neurological deficit score of the rats in the model group was significantly increased(P<0.01),and the number of survival neurons of the ischemic cortex was obviously decreased(P<0.01),and the expression levels of nNOS,iNOS and GFAP were significantly increased in the model group(P<0.01).In the EA preconditioning group,the neurological deficit score,the expression levels of nNOS and iNOS were significantly down-regulated(P<0.01),while the number of the survival neurons and GFAP expression level in the ischemic cerebral cortex were obviously higher in the EA preconditioning group in compared with the model group(P<0.01).Conclusion EA preconditioning can protect the ischemic cerebral cortex tissue from injury in CI/R rats,which may be related to its effects in down-regulating the expression of nNOS and iNOS,and up-regulating the expression of GFAP.关键词：Cerebral ischemia-reperfusion;Electroacupuncture preconditioning;Cerebral Neuronal nitric oxide synthase;Inducible nitric synthase;Glial fibrilliary acidic protein940|344|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupotomy(needle-knife)therapy on local pathological changes and cartilage-mechanics related protein expression in rabbits with knee osteoarthritis(KOA)so as to study its mechanisms underlying improving KOA.Methods Forty New Zealand rabbits were randomly divided into normal control group,model group,acupotomy group,and electroacupuncture(EA)group(n=10in each group).The KOA model was established by immobilization of the left knee-joint(modified Videman method)for 6weeks.After modeling,acupotomy relaxing was applied to the lateral collateral ligament and patellar ligament of the left knee-joint,once a week for 3times,and EA(2Hz/100 Hz,3mA)was applied to the left"Yanglingquan"(GB 34),"Yinlingquan"(SP 9),"Neixiyan"(EX-LE 4)and"Waixiyan"(ST 35)for 20 min,3times a week for three weeks.The expression levels of Integrinβ1,type II collagen(Col-Ⅱ),matrix metalloproteinase(MMP)-3and Aggrecan proteins of the cartilage tissue of the left femoral medial and external condyles were observed by Western blot.Pathological changes of the knee-joint by X-ray scanning and those of the femoral condyle tissue were evaluated by Mankin's scores under light microscope after H.E.staining.Results X-ray showed successful modeling,and pathological changes of the articular cartilage belonged to the early and moderate lesion of knee osteoarthritis.The Mankin's score was significantly higher in the model group than in the control group(P<0.01),and after the treatment,the Mankin's scores were significantly decreased in the acupotomy group(P<0.01),rather than in the EA group(P>0.05).The results of Western blot showed that after modeling,the expression levels of Integrinβ1,Col-Ⅱ and Aggrecan proteins of the femoral articular cartilage were considerably decreased(P<0.01),while that of MMP-3protein was significantly increased(P<0.01).Compared with the model group,the decreased expression levels of Integrinβ1,Col-Ⅱand Aggrecan proteins in the acupotomy group and Integrinβ1protein in the EA group were notably up-regulated(P<0.01,P<0.05),and MMP-3expression in the acupotomy group was significantly down-regulated(P<0.01).No significant changes were found in the EA group in the expression levels of Col-Ⅱ,Aggrecan and MMP-3proteins compared with the model group(P>0.05).Conclusion Acupotomy intervention can relieve KOA-induced injury of the kneejoint in KOA rats,which is associated with its actions in raising expression levels of Integrinβ1,Col-Ⅱ and Aggrecan proteins and in lowering the expression of MMP-3proteins in the articular cartilage,probably by adjusting the mechanics-related signal pathway of the articular chondrocytes.965|1126|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of low-frequency electroacupuncture(EA)treatment on oxidative stress and metabolism disorder in polycystic ovary syndrome(PCOS)rats,so as to study its underlying mechanisms in improving PCOS.Methods Thirty female SD rats were randomized into control group,model group and EA group(n=10in each group).The PCOS model was established by subcutaneous injection of testosterone propionate(1mg/100 g,10mg/mL)for 8weeks.EA(2Hz)was applied to"Zhongwan"(CV 12),"Guanyuan"(CV 4)and bilateral"Sanyinjiao"(SP 6)or"Housanli"(ST 36)for 30 min,once daily(except weekends)for 5weeks.Serum testosterone(T),sex hormone-binding globulin(SHBG)and fasting serum insulin(FINS)contents were detected by ELISA,serum malondialdehyde(MDA)and superoxide dismutase(SOD)levels were assayed by thiobarbituric acid(TBA)and xanthine oxidation(XTO)methods,respectively,and fasting blood glucose(FBG)was determined by roche glucose meter.In addition,homeostasis model assessment for insulin resistance(HOMA-IR)and modified B cell function index(MBCI)were calculated.Results Compared with the control group,serum T,FBG,FINS,HOMAIR,and serum MDA levels were significantly increased in the model group(P<0.01),while serum SHBG,MBCI and SOD levels were considerably decreased in the model group(P<0.01).Following EA treatment,all the increased serum T,FBG,FINS,HOMA-IR,and serum MDA levels,and the decreased serum SHBG,MBCI and SOD levels were reversed obviously(P<0.05,P<0.01).Conclusion EA treatment may normalize insulin sensitivity,ameliorate insulin resistance and hyperinsulinemia in PCOS rats,probably by regulating the function of pancreatic isletsβcell and by reducing oxidative stress and free androgen.关键词：Electroacupuncture;Polycystic ovary syndrome;Insulin resistance;Oxidative stress902|675|8更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture intervention on gastric ulcer(GU)and sleeping quality from the viewpoint of brain-gut axis which plays an important role in the regulation of many vital functions in the body.Methods Forty male Wistar rats were randomized into normal control,GU model,acupuncture of"Zhongwan"(CV 12)-"Zusanli"(ST 36,gastric function regulating acupoints),acupuncture of"Shenmai"(BL 62)-"Zhaohai"(KI 6,sleep-promotion acupoints),and acupuncture of CV 12-ST 36+BL 62-KI 6(combined treatment)groups,with 8rats in each group.GU model was established by intragastric perfusion of dehydrated alcohol(1mL/rat),and sleep model established by intraperitoneal injection of pentobarbital sodium(40mg/kg)after the last treatment.The abovementioned acupoints were punctured with filiform needles and stimulated by manipulating the needle for about 30 s,once every 5min during 20 min of needle retention.The treatment was conducted once daily for five days.The contents of tumor necrosis factor-alpha(TNF-α)and interleukin-25(IL-25)in the serum and hippocampal tissues were detected by ELISA.Results Compared with the normal control group,the gastric ulcer index score,barbiturate-induced sleeping time,and TNF-αand IL-25 contents in both serum and hippocampus were significantly increased in the model group(P<0.01).Following acupuncture treatment,in comparison with the model group,the gastric ulcer index score,barbiturate-induced sleeping time,and TNF-αand IL-25 contents in both serum and hippocampus were significantly down-regulated in the CV 12-ST 36,BL 62-KI 6and combined treatment groups(P<0.01,P<0.05).The effects of the CV 12-ST 36 and combined treatment groups were remarkably superior to those of the BL 62-KI 6group in down-regulating ulcer index score,serum IL-25,and hippocampal TNF-αand IL-25contents(P<0.01,P<0.05).In addition,the effects of the BL 62-KI 6and combined treatment groups was considerably better than that of the CV 12-ST 36 group in shortening barbiturate-induced sleeping time(P<0.01,P<0.05).The effect of the combined treatment group was markedly better than that of the CV 12-ST 36 and BL 62-KI 6groups in lowering serum TNF-αcontent(P<0.05).Conclusion Acupuncture stimulation of CV 12,ST 36,KI 6and BL 62 can relieve the gastric mucosal lesion,and shorten barbiturate-induced sleeping time in gastric ulcer rats,which may be related to its effects in reducing TNF-αand IL-25 contents in the serum and hippocampus tissues,suggesting a correlation between the gastrointestinal disorder and sleeping.关键词：Gastric mucosal injury;Acupuncture intervention;Sleep duration;Gastric mucosal lesion index;Tumor necrosis factor-alpha;Interleukin-25891|365|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of twirling-reinforcing or reducing needling manipulations on plasma acetylcholine(Ach)content and expression of nitric oxide synthetase(NOS)and cyclic guanosine monophosphate(cGMP)in thoracic artery tissue in stress-induced hypertension rats.Methods A total of 60 male rats were randomly divided into blank control,model,acupuncture(no-needle-manipulation),twirling-reinforcing needling and twirling-reducing needling groups(n=12in each group).The stress hypertension model was established by giving the animals with noise and electric shock stimulation(paw),twice a day for15 days.Acupuncture stimulation was applied to bilateral"Taichong"(LR 3)for 1min,followed by retaining the needles for 20 min.The treatment was conducted once daily for 7days.Systolic blood pressure of the rat's tail was detected with non-invasive method and plasma Ach,and NOS and cGMP contents in the thoracic artery tissue were measured using ELISA method.Results Compared with the control group,the systolic blood pressure was significantly higher in the model group after 15days' stress stimulation(P<0.01),while the contents of plasma Ach,arterial NOS and cGMP were markedly down-regulated(P<0.01).Following 7days' acupuncture interventions,the increased blood pressure was down-regulated in the no-needle-manipulation,twirling-reinforcing needling and twirling-reducing needling groups(P<0.05,P<0.01);and the decreased Ach and NOS in the 3treatment groups,and cGMP levels in the twirling-reinforcing and twirling-reducing needling groups were remarkably up-regulated(P<0.01,P<0.05).No significant change of arterial cGMP content was found in the no-needle-manipulation group(P>0.05).The effect of the twirling-reducing needling was superior to that of no-needle-manipulation and twirling-reinforcing needling in lowering blood pressure and raising plasma Ach content(P<0.05,P<0.01).Conclusion The twirling-reducing needling of acupuncture has a significant anti-hypertensive effect in stress hypertension rats,which may be associated with its effects in raising blood Ach,and arterial NOS and cGMP levels.895|471|9更新时间：2023-08-11
- 摘要：Objective To observe the influence of electroacupuncture(EA)stimulation of"Zusanli"(ST 36)and"Kunlun"(BL 60)on serum and knee-joint IL-17 and IL-23 contents in rheumatoid arthritis(RA)rats so as to study its underlying mechanism in improving RA.Methods Male Wistar rats were randomly allocated to normal control,RA model,EA and Prednisolone groups(n=8in each group).The RA model was established by intra-planta injection of incomplete Freund's adjuvant and type Ⅱ collagen.EA(2Hz/100 Hz,1-2mA)was applied to bilateral"Zusanli"(ST 36)and "Kunlun"(BL 60)for 30 min,once daily for 10 days.The rats' ankle diameter was measured,and IL-17 and IL-23 contents in the serum and the knee-joint cavity were assayed by ELISA and Western blot,respectively.Results In comparison with the normal control group,the rats' ankle diameter,serum IL-17 and IL-23 contents and knee-joint IL-17 and IL-23 protein expression levels were significantly increased in the model group(P<0.05).After EA and Prednisolone treatment,compared with the model group,all the rats' ankle diameter,serum IL-17 and IL-23 contents and knee-joint IL-17 and IL-23 protein expression levels were decreased remarkably(P<0.05).No obvious differences were found between the EA and Prednisolone groups in the aforementioned indexes(P>0.05),except IL-17 protein expression level(being markedly lower in the Prednisolone group than in the EA group).Conclusion EA intervention can reduce inflammatory reaction of the ankle-joint in RA rats,which may be related to its effects in down-regulating serum and knee-joint IL-17 and IL-23 levels.关键词：Electroacupuncture;Rheumatoid arthritis;IL-17;IL-23926|511|8更新时间：2023-08-11
- 摘要：Objective To observe the effect of different-frequency-electroacupuncture(EA)and moxibustion stimulation on rabbits' arrhythmias.Methods Newzealand rabbits were randomly divided into arrhythmia model(n=18),EA-3Hz(n=19),EA-20Hz(n=19),EA-100Hz(n=18)and moxibustion(n=18)groups.The arrhythmia model was induced by intravenous injection of 25μg/kg aconitine.EA(3Hz,20 Hz or 100 Hz,3.5-4V)or moxibustion was applied to bilateral"Neiguan"(PC 6)for 20 min and electrocardiogram(ECG)of the standard limb leadⅡ was recorded by using a Biosignal Processing Instrument.The concentration of serum hypersensitive C reactive protein(hs-CRP)content was detected by using ELISA method.Results Following EA intervention,the duration of aconitine-induced arrhythmia was significantly shorter in both EA-20 Hz and EA-100 Hz groups than in the model group(P<0.05,P<0.01),and the serum hs-CRP level in the EA-20 Hz group was significantly lower than that of the model group(P<0.05).No significant changes were found in the duration of arrhythmia in both EA-3Hz and moxibustion groups,and in the serum hs-CRP level in the EA-3Hz,EA-100 Hz and the moxibustion groups(P>0.05).Conclusion EA stimulation of PC 6at 20 Hz and 100 Hz has an anti-arrhythmic effect in aconitine-induced arrhythmia rabbits.关键词：Arrhythmia;Electroacupuncture intervention;Stimulation frequency;Hypersensitive C reactive protein919|463|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)treatment on the quality of ovum,stem cell factor(SCF)and the pregnancy outcome in patients with polycystic ovarian syndrome(PCOS),so as to explore its mechanism underlying improving pregnancy rate.Methods A total of 200 PCOS patients undergoing in vitro fertilization-embryo transplantation(IVF-ET)were randomly divided into control(medication)group(n=98)and EA group(n=102).For patients of the EA group who were undergoing controlled ovarian hyperstimulation,EA stimulation(5Hz/20 Hz,15-20V)was applied to bilateral Shenshu(BL 23),Qihai(CV 6),bilateral Zusanli(ST 36)-Sanyinjiao(SP 6),and bilateral Neiguan(PC 6)-Zigong(EX-CA 1)for30min,once daily till accepting embryo transplant.Patients of the medication group were treated with controlled ovarian hyperstimulation using Diane-35,Decepepty,Gonadotrophin,human Chorionic Gonadotrophin,etc.Serum follicle-stimulating hormone(FSH),luteinizing hormone(LH),testosterone(T),estradiol(E2),and progesterone(P)contents were detected using chemiluminescent method.SCF contents in the serum and follicular fluid were assayed by ELISA.The number of retrieved oocytes,fertilization rate,cleavage rate,high quality embryo rate,ovarian hyperstimulation syndrome(OHSS)incidence rate,cycle cancellation rate,cli-nical pregnancy rate,early abortion rate,Gn dosage and administration duration,and the correlation between the high quality embryo rate and SCF level were determined.Results Comparison between the two groups showed that the high quality embryo rate,and serum and follicular fluid SCF contents were significantly higher in the EA group than in the medication group(P<0.05,P<0.01),while the dosage and administration duration of Gn were significantly lower in the EA group than in the medication group(P<0.05).A positive correlation was found between the high quality embryo rate and the SCF level in both follicular fluid and serum(P<0.01).No significant differences were found between the two groups in the number of retrieved oocytes,fertilization rate,cleavage rate,clinical pregnancy rate,OHSS incidence rate,cycle cancellation rate,early abortion rate,serum LH,E2 and P contents(P>0.05).Conclusion EA can improve the high quality embryo rate,which may be related to its effect in increasing serum and follicular fluid SCF levels.关键词：Polycystic ovary syndrome;Electroacupuncture;Controlled ovarian hyperstimulation;Stem cell factor;High quality embryo rate825|928|12更新时间：2023-08-11
- 摘要：Objective To observe the clinical therapeutic effect of blood-letting plus acupuncture intervention for acute facial paralysis with pain(damp-heat type of liver and gallbladder).Methods A total of 120 cases of acute facial paralysis with pain were randomly and equally divided into control group and treatment group.Patients of the control group were treated by simple acupuncture therapy only,and patients of the treatment group treated by acupuncture plus bloodletting at the ipsilateral Yangbai(GB 14)and Quanliao(SI 18),and Dazhui(GV 14)and Yifeng(TE 17),alternatively.Shallow-acupuncture stimulation of ipsilateral Cuanzhu(BL 2),Sizhukong(TE 23),Sibai(ST 2),Dicang(ST 4),Jiache(ST 6),Yingxiang(SP 9),and bilateral Hegu(LI 4),Yanglingquan(GB 34)and Xingjian(LR 2)was administered for 30 min,once daily for one month.The patients' facial pain degree was evaluated by visual analogue scale(VAS),and the pain duration during acute stage was recorded.The facial nerve function and facial nerve function recovery(grade II)time were determined by using Sunnybrook Facial Grading scale and House-Brackmann(HB)grading scale,respectively.Results In comparison with pre-treatment,the post-aurem pain scores after the first treatment and after the acute stage were significantly decreased,and the facial nerve function scores after the treatment were significantly increased in both control and treatment groups(P<0.01),and the effects of the treatment group were significantly superior to those of the control group(P<0.01).The post-aurem pain duration and the time of facial nerve function recovery(HB)of the treatment group were considerably lower than those of the control group(P<0.01).Of the 58 and 59facial palsy patients in the control and treatment groups,16(27.6%)and 24(40.7%)were cured,18(31.0%)and 23(39.0%)experienced marked improvement,15(25.9%)and 10(16.9%)were improved,and 9(15.5%)and 2(3.4%)were failed,with the effective rate being 85.0% and 96.6%,respectively.Conclusion Bloodletting combined acupuncture is effective in the treatment of acute facial paralysis with pain.关键词：Facial palsy(acute stage);Bloodletting;Acupuncture;Hepatobiliary damp heat type845|510|3更新时间：2023-08-11
- 摘要：The Ziwuliuzhu(midnight-noon ebb-flow)needling of acupuncture theory was generally considered to be a representative perfect example of traditional acupuncture theory.It is created in the Jin and Yuan Dynasties,and has been being frequently cited thereafter.It is mainly derived from and inherits the theory of Wuxing(five-elements)doctrine of traditional Chinese culture in ancient China,for which the Wushu-points,five specific acupoints on the distal ends of the arm and leg,i.e.,Jing(Well-points),Xing(Spring-point),Shu(stream-point),Jing(river-point)and He(sea-point)to match the Wuxing so as to calculate their open time according to the Heavenly Stems and Earthly Branches designating the years,months,days and hours in ancient China.Although it has been repeatedly copied in Chinese medical books,this theory was not really used by clinical acupuncturists in the Jin,Yuan and following dynasties.The present theory came up in the special time,primarily being derived from the combination of medical and Confucian theories in the Song Dynasty.Nowadays,we should thoroughly make known its connotations,and do not make heedless affirmation and clinical validation.关键词：Ziwuliuzhu;Acupuncture theory;Academic history825|1472|11更新时间：2023-08-11
- 摘要：In our previous paper,we analyzed"Deqi"in book Huangdi Neijing(The Yellow Emperor's Internal Classic)and Nanjing(Canon of Difficult Medical Problems)from "Zhishen"(Treating mentality)and Tiaoqi(Regulating qi).In the present paper,the authors discuss the connotations of"Deqi"and related events in the later ages of the abovementioned two classic books to the later stage of the Qing Dynasty when involves about 20 classical works as Zhenjiu Dacheng(The Great Compendium of Acupuncture and Moxibustion),Zhenjing Zhinan(Guide to the classics of Acupuncture),Zhenjiu Daquan(A Complete Works of Acupuncture and Moxibustion)etc.from 1)close association between"Deqi"and patients' mental activity;2)how to wait for arrival of qi if the needling does not induce"Deqi"for the time being;3)how to identify"qi-arrival"and then,performing suitable manipulations;4)Deqi and shallow-or deep-needling;5)putting more emphasis on patients' feeling and reactions,rather than the practitioner's perception beneath the needle which is described in book Huangdi Neijing;and 6)not withdrawing the acupuncture needles if qi does not arrive.Generally,in the later ages,the connotations of Deqi are enriched greatly.关键词：Acupuncture;Deqi;Developed connotations;Ancient classical medical works914|753|2更新时间：2023-08-11
- 摘要：Objective To sum up the application regularity of Fengfu(GV 16)acupoint in clinical practice through studying the ancient literature from the early stage of the Qin Dynasty to the Qing Dynasty.Methods Chinese ancient medical literature relevant to Fengfu(GV 16)was searched to establish a database containing information of clinical indications of GV 16,supplementary acupoints,relevant needling techniques,moxibustion methods,etc.Results A total of 277 articles about Fengfu(GV 16)for 61 types of clinical disorders or diseases involving internal medicine,surgery,paediatrics,five sense organs,etc.were obtained from 2 200 ancient Chinese medical books.Fengfu(GV 16)alone was most frequently used for treatment of disorders caused by exogenous factors,such as common cold,neck pain,headache,epilepsy,mania,dizziness,throat dumb,legfoot problems,etc.,with the auxiliary acupoint being Fengchi(GB 20).In addition,147 articles relevant to needling and moxibustion stimulation of GV 16(7types of methods)were found.Conclusion Fengfu(GV 16)is mainly used for local problems in the human body,and also for problems occurred along the meridian trace,aiming at the pathogenesis.When employed,GV 16 is often stimulated by acupuncture needle.关键词：Fengfu(GV 16);Acupoint;Regularity of clinical application;Literature research826|985|3更新时间：2023-08-11
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