最新刊期
卷 40 ， 期 5 ， 2015
- 摘要：Objective To observe the effect of electroacupunctrue(EA)intervention or EA combined with dietary control on peroxisome proliferator-activated receptor(PPAR)-α,and liver fatty acid-binding protein(L-FABP)levels in non-alcoholic fatty liver disease(NAFLD)rats,so as to reveal its mechanism underlying improvement of NAFLD.Methods Sixty SD male rats were randomly divided into common diet(control)group(n=10)and high-fat diet group(n=45).The NAFLD model was established by feeding the animals with high-fat forage(HFF,including cholesterol,sodium cholate,propylthiouracil,sucrose,lard and common forage)for 5weeks.Forty NAFLD rats were then randomized into model,EA+HFF,low-fat forage(LFF)and EA+LFF groups(n=10rats in each group).EA(4Hz/20 Hz,3mA)was applied to ipsilateral"Zusanli"(ST 36),"Sanyinjiao"(SP 6)and"Taichong"(LR 3)for 20 min,once daily for 4weeks.The pathologic changes of the hepatic tissue were detected by H.E.staining.Serum total cholesterol(TC)and triglyceride(TG)contents were determined by using enzymatic methods,serum free fat acids(FFA)content was detected by colorimetry.The expression levels of PPAR-αand L-FABP protein and gene of the liver tissue were determined by Western blot and RT-PCR,respectively.Results H.E.staining showed that the hepatocytes presented moderate or severe bullous adipose degeneration in rats of the model group,vesicular steatosis in the EA+HFF and LFF groups,turned to almost normal but with small amount of lipid droplets in the EA+LFF group.The contents of serum TC,TG and FFA were significantly higher in the model group than in the control group(P<0.05),and were obviously decreased in the EA+HFF,LFF and EA+LFF groups in comparison with the model group(P<0.05).Compared to the control group,hepatic PPAR-αprotein and mRNA were markedly down-regulated in the model group,and hepatic L-FABP protein and mRNA considerably up-regulated in the model group(P<0.05).Following EA intervention and low fat diet feeding,PPAR-αprotein and mRNA were markedly up-regulated while L-FABP protein and mRNA remarkably down-regulated in the EA+HFF,LFF and EA+LFF groups(P<0.05).Conclusion EA intervention or EA combined with dietary control can relieve hepatic pathological changes in NAFLD rats,which may be related to its effects in down-regulating blood lipid level and hepatic L-FABP protein and mRNA expression and in up-regulating PPAR-αprotein and mRNA expression,and the effect of combined treatment was relatively better than simple EA or LFF treatment.关键词：Non-alcoholic fatty liver disease;Electroacupunctrue therapy;Dietary control;Peroxisome proliferator-activated receptor-α;Liver fatty acid-binding protein821|238|12更新时间：2023-08-11
- 关键词：Cervical spondylosis;Needle-knife;Cervical acupoints;Extracellular matrix;Ultrastructure;Pulpi-form nucleus983|456|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)stimulation of"Weizhong"(BL 40)-"Huantiao"(GB 30)on expression of phosphorylated calcium/calmodulin dependent protein kinaseⅡ(p-CaMKⅡ)and cAMP response element binding protein(p-CREB)in the spinal cord in rats with spared nerve injury(SNI),so as to explore its mechanism underlying easing neuropathic pain.Methods Sixty SD rats were randomly divided into five groups:control(sham-operation),model,EA,AP-5(a NMDA receptor antagonist)and L-NAME(a non-selective nitric oxide synthase,NOS inhibitor)(n=12in each group).The neuropathic pain model was established by sectioning the right tibal nerve and common peroneal nerve.EA intervention(2Hz,1mA,increasing 1mA/10min)was applied to"Weizhong"(BL 40)and"Huantiao"(GB 30)on the injured side for30 min,once a day for 7days.Rats of the AP-5and L-NAME groups were treated by intragastric administration of AP-5(0.7mg·kg-1·d-1)and L-NAME(60mg·kg-1·d-1)respectively from the 11 th day after operation,once daily for 7days.The mechanical pain thresholds were measured before the SNI procedure(baseline)and at the 10 th and 16 th day after the procedure.The expression of p-CaMKⅡ protein and p-CREB protein and gene of the spinal cord(L4-L6segments)was determined by Western blot and fluorescence quantitative-polymerase chain reaction(PCR),separately.Results In comparison to the control group,the mechanical pain threshold was significantly decreased in the model group(P<0.01).After EA intervention,the mechanical pain thresholds of the EA,AP-5and L-NAME groups were obviously increased(P<0.01,P<0.05)on day 16 post SNI procedure.The expression levels of p-CaMKⅡand p-CREB proteins and CREB mRNA in the spinal cord were significantly higher in the model group than in the control group(P<0.05).Compared with the model group,the expression levels of spinal p-CaMKⅡand pCREB proteins and CREB mRNA were obviously down-regulated in the EA group(P<0.05),but not in the AP-5group and the LNAME group(P>0.05).Conclusion EA intervention of BL 40-GB 30 may alleviate pain in neuropathic pain rats,which may be related to its effects in down-regulating spinal CaMKⅡ-CREB pathway function.关键词：Electroacupuncture intervention;Neuropathic pain;Spinal cord;CaMKⅡ;CREB876|363|7更新时间：2023-08-11
- 摘要：Objective To observe the action of electroacupuncture(EA)intervention on the expression of gap junction protein connexin 43(Cx 43)and content of glutamate(Glu)in the striatum in Parkinson's disease(PD)rats,so as to reveal its mechanism underlying improvement of PD.Methods Forty male SD rats were randomly divided into normal control,sham operation,model and EA groups(n=10in each group).The PD model was duplicated by microinjection of 6-hydroxyldopamine(6-OHDA,15μg/rat)into the right striatum of rats(AP:1.0,1.0;R:3.0,4.5;H:4.5,6.0),and for control,the same dose of normal saline was injected into the right striatum for rats in the sham operation group.EA(2Hz,1mA)was applied to"Fengfu"(GV 16)"Taichong"(LR 3)for 30 min,once a day for 2weeks.The PD rats' rotational behavior changes(the numbers of rotations in 30min)were detected following subcutaneous injection of apomorphine(0.5mg/kg).The Glu concentration and the expression of Cx 43 in the striatum were detected by using high performance liquid chromatography(HPLC)and Western blot,respectively.Results No significant differences were found between the model group and EA group in the number of rotations before the treatment,between the control and sham operation groups in the levels of Glu content and Cx 43 protein expression in the striatum(P>0.05).Compared with the control group,the Glu content and Cx 43 protein expression level were significantly increased in the model group(P<0.01),while in comparison with the model group,the number of rotations was significantly reduced in the EA group(P<0.05).Following EA intervention,both Glu content and Cx 43 expression were considerably downregulated in the EA group compared with the model group(P<0.05,P<0.01).Conclusion EA can improve PD rats' rotation behavior,which may be associated with its effects in down-regulating the level of Glu and Cx 43 protein expression in the striatum.关键词：Parkinson's disease;Eelectroacupuncture intervention;Striatum;Cx 43;Glutamate834|448|5更新时间：2023-08-11
- 摘要：Objective To observe the influence of acupuncture intervention on the expression of pivotal pro-inflammatory molecules nuclear factor-κB(NF-κB),cyclooxygenase-2(COX-2),and prostaglandin E2(PGE2)of NF-κB inflammatory signal pathway in the hippocampus of depression rats,so as to explore its mechanism underlying improvement of depression.Methods Thirty-six male Sprague-Dawley rats were randomly divided into four groups:control,model,acupuncture,and medication(fluoxetine,Flu)(n=9rats in each group).The depression model was established by using chronic unpredictable stress stimuli combined with solitary feeding for 28 days.Acupuncture treatment was applied to"Baihui"(GV 20)and"Neiguan"(PC 6)once every other day for 28 days.Fluoxetine was given to rats of the medication group(10mg/kg)by gavage(p.o.)everyday for 28 days before the stress stimulation.The expression of NF-κB protein in the hippocampus tissue was detected by Western blot,and the contents of COX-2and PGE2 were detected by ELISA.Results Compared to the control group,the levels of NF-κB protein expression,COX-2and PGE2 contents in the hippocampus were significantly increased in the model group(P<0.01).Compared to the model group,the levels of NF-κB protein expression,COX-2and PGE2 contents in the hippocampus were significantly downregulated in both the acupuncture and medication groups(P<0.05,P<0.01).There was no significant differences between the acupuncture and medication groups in the NF-κB protein expression,and COX-2and PGE2contents(P>0.05).ConclusionAcupuncture intervention can effectively reduce hippocampal NF-κB expression,and COX-2and PGE2 levels in depression rats,which may contribute to its anti-depressant effect by inhibiting pro-inflammatory pathway to protect the neurons of hippocampus.关键词：Acupuncture intervention;Depression;Chronic stress;Hippocampus;Pro-inflammatory pathway;NF-κB protein983|589|6更新时间：2023-08-11
- 摘要：Objective To observe the effects of electroacupuncture(EA)stimulation of different tissues(nerve stem,muscular layer)at"Huantiao"(GB 30)acupoint on expression of phosphorylated c-jun N-terminal kinase(p-JNK)and c-jun(pc-jun)proteins in the lumbar spinal cord in rats with sciatic nerve injury,so as to explore its mechanism underlying improvement of peripheral neuropathic damage.Methods Forty-eight SD rats were randomly divided into normal,model(the left sciatic nerve severed),GB 30 deep needling(the acupuncture needle tip was inserted to the sciatic nerve trunk to elicit an instantaneous jerk of the hind limb)and GB 30 shallow needling(the needle tip was inserted to the muscle layer to evoke a local muscular contraction)groups(n=12rats in each group).EA stimuli were delivered at 2Hz/100 Hz,1mA,20 min in duration per treatment for 10 consecutive days.Histo-pathological changes were observed by Hematoxylin-eosin(HE)staining and immunohistochemical assay was carried out to examine the pathological change of spinal segments(L4-L5)and the expression of p-JNK and p-c-jun proteins,respectively.Results For rats with the sciatic nerve severed,the spinal neurons became swelling,degeneration or even apoptosis.Acupuncture intervention reduced the number of apoptosic neurons and improved the pathological change,which was relatively better in the deep needling group than in the shallow needling group.Likewise,the elevated spinal p-JNK and p-c-jun expression levels of the model group were significantly reduced by EA intervention(deep needling vs shallow needling,P<0.01).Conclusion Acupuncture can improve the spinal pathological changes in rats with sciatic nerve injury,which is probably achieved by decreasing the p-JNK and p-c-jun expression and inhibiting the JNK signaling pathway,and thereby,reducing the apoptosis of the spinal neurons.Deep needling results in greater benefits than shallow needling.关键词：Sciatic nerve injury;Deep electroacupuncture stimulation;Huantiao(GB 30)point;JNK;c-jun737|518|15更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)stimulation of"Neiguan"(PC 6)at different frequencies on plasma vasoactive substance levels in myocardial ischemia-reperfusion(MIR)injury rats,so as to explore its mechanisms underlying improvement of acute myocardial ischemia.Methods A total of 40 Wistar rats were randomized into control,model,high frequency(HF,120Hz)and low frequency(LF,20Hz)groups(n=10in each group).The MIR model was established by occlusion of the anterior descending branch(ADB)of the left coronary artery for 30 min,followed by reperfusion for40 min.EA(3V,120 Hz or 20Hz)was applied to bilateral"Neiguan"(PC 6)for 50 min immediately after occlusion of ADB.Subsequently,the contents of plasma endothelin(ET),atrial natriuretic peptide(ANP),thromboxane B 2(TXB2)and 6-Keto-PGF1αwere assayed by radioimmunoassay,and the content of serum nitric oxide(NO)was detected by nitrate reductase method.Results Compared with the control group,the contents of plasma ET,ANP and TXB2 in the model group were significantly increased(P<0.05),and that of plasma 6-Keto-PGF1αin the model group was notably decreased(P<0.05),but no significant change was found in serum NO level(P>0.05).Compared with the model group,the contents of plasma ET,ANP and TXB2 were considerably decreased,and plasma 6-Keto-PGF1α and serum NO contents were obviously increased in both HF and LF groups(P<0.05).No significant differences were found between the HF and LF groups in plasma ET,ANP,TXB2 and 6-KetoPGF1αcontents(P>0.05),but the HF EA was markedly superior to the LF EA in up-regulating the content of serum NO(P<0.05).Conclusion EA stimulation of"Neiguan"(PC 6)can down-regulate the contents of plasma ET,ANP and TXB2 and up-regulate contents of plasma 6-Keto-PGF1αand serum NO in MIR rats,which may contribute to its effect in relieving acute ischemic myocardial injury.The effect of HF EA is better than LF EA in raising blood NO level.关键词：Myocardial ischemia-reperfusion;Vasoactive substances;Electroacupuncture frequencies864|593|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture intervention at different time-points on the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),the content of malonaldehyde(MDA)and expression of heat shock protein 70(HSP 70)of liver tissue in rats with simulated weightlessness,so as to explore its mechanism underlying improvement of liver injury in rats with simulated weightlessness.Methods Twenty male Wistar rats were randomly divided into control group,model group,pre-acupuncture group and EA group,5rats in each.The model of simulated weightlessness was established by tail suspension for 4week.One week before the tail suspension,the rats in the pre-acupuncture group were treated with electroacupuncture(EA)at"Shenshu"(BL 23),"Pishu"(BL 20)and "Sanyinjiao"(SP 6)for 30 min before treatment,once a day for 7days.The rats in the EA group received tail suspension and acupuncture intervention at the same time.EA was applied for 30 min per treatment,once every other day for 14 times.Immunohistochemical staining was used to assay the expression of HSP 70 in the liver tissue.The activities of SOD and GSH-PX and content of MDA in liver tissues were examined by means of colourimetric method.Results Compared with the control group,the expression of HSP 70 and the content of MDA in the liver tissue were increased significantly(P<0.01),and the activity of SOD and GSH-PX was notably reduced(P<0.05)in the model group.Compared with the model group,the content of HSP 70 was significantly reduced in the pre-acupuncture group(P<0.01).There were no significant changes in the levels of SOD,GSH-PX,MDA and HSP 70 in the EA group(P>0.05).In comparison with the pre-acupuncture group,the activity of GSH-PX was lower(P<0.05)and the content of MDA was higher(P<0.05)in the EA group.Conclusion EA-pretreatment can suppress the increase of liver HSP 70 immunoactivity in rats with simulated weightlessness,being likely to improve the antioxidant ability of liver.关键词：Acupuncture;Simulated weightlessness;Liver injury;Heat shock protein 70;Superoxide dismutase;Glutathione peroxidase;Malonaldehyde874|330|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of suspending-moxibustion stimulation of"Dazhui"(GV 14)with different quantities on the levels of nerve growth factor(NGF),substance P(SP),calcitonin gene-related peptide(CGRP),neurokinin A(NKA),neurokinin B(NKB)and phosphorylated extracellular signal-regulated kinases(pERK)in the bronchoalveolar lavage fluid(BALF)of asthma rats,so as to analyze its mechanisms underlying improving asthma.Methods Sixty male SD rats were randomly divided into six groups:blank control,model,15min-moxibustion(15min-moxi),30min-moxi,60min-moxi and 90minmoxi(n=10rats in each group).The asthma model was established by intraperitoneal injection of suspension of egg protein,magaldrate,and inactivated Bacillus pertussis(on day 1and 8),and inhaling the atomized ovalbumin saline(from day 15 on for 14days).Mild moxibustion was conducted at"Dazhui"(GV 14)for 15 min,30min,60 min and 90 min,respectively,once daily for7 days.The levels of NGF,SP,CGRP,NKA,NKB,and pERK in the BALF were detected by ELISA(enzyme-linked immuno sorbent assay).Results The contents of NGF,SP,CGRP,NKA,NKB and pERK in the BALF in the model group were obviously higher than those in the blank control group(P<0.01),suggesting an apparent inflammatory reaction in rats after modeling.Following moxibustion,the levels of NGF,SP,CGRP,NKA,NKB and pERK of the four treatment groups were significantly downregulated compared with the model group(P<0.01).The effect of 30min-moxi group was obviously superior to that of 15 minmoxi group(P<0.01),and those of 60min-moxi and 90min-moxi groups were markedly superior to those of 15min-moxi and 30min-moxi groups(P<0.01)in down-regulating NGF,SP,CGRP,NKA,NKB,and pERK levels in the BALF.No significant differences were found between the 60min-moxi and 90min-moxi groups in down-regulating NGF,SP,CGRP,NKA,NKB,and pERK levels(P>0.05).Conclusion Suspending-moxibustion stimulation of GV 14 can down-regulate the contents of NGF,SP,CGRP,NKA,NKB,and pERK levels in the BALF in asthma rats,suggesting a relief of neurogenic inflammation reaction after moxibustion.The effect of moxibustion presents a time-dependant manner and peaks at 60 min.关键词：Suspending moxibustion;Asthma;Neurogenic inflammation mediators;Bronchoalveolar lavage fluid902|428|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of preconditioning of catgut implantation on changes of behavior and EEG of rats with epilepsy.Methods Forty male Wistar rats were randomly divided into control,model,catgut implantation,medication groups(10rats/group).The catgut implantation was performed at"Yintang"(EX 2)to"Baihui"(GV 20)and"Changqiang"(GV 1)on day 1,4and 7.The epileptic model was made by intraperitoneal injection of penicillin sodium on the last day of the treatment.In the medication group,intragastric administration of valproic acid sodium was conducted once a day for consecutive 7days.Behavior measurement and EEG recording were carried out shortly after the penicillin administration.Results Compared with the model group,the seizure latency was notably increased in the catgut implantation group and the medication group(P<0.01),without significant difference between the last two groups(P>0.05).The degree of the seizure attack was less severe in the catgut implantation group and the medication group(P<0.01),without significant difference between the two groups(P>0.05).Similar to valproic acid sodium,catgut implantation significantly reduced the amplitude and accelerated the frequency of EEG(P<0.01).Conclusion Catgut implantation can delay the seizure attack latency,alleviate the severity,regulate the amplitude and frequency of the epileptiform discharges,giving rise to evident antiepileptic effect.关键词：Preconditioning of catgut implantation;Epilepsy;Behavior;Electroencephalogram(EEG)953|338|2更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture and moxibustion intervention on the contents of serum IL-7and IL-18 and white blood cell counts in cyclophosphamide(CTX)-induced hypofunction of immunity in tumor-bearing mice,so as to explore its mechanism underlying improvement of immune suppression caused by chemotherapy.Methods Sixty-four male Kunming mice were implanted with S 180 sarcoma at the left armpit(for making tumor-bearing model),and then,randomized into control group,model group,acupuncture group and moxibustion group(16mice in each group).The mice of the latter 3groups were injected with CTX(i.p.,150mg·kg-1·d-1),and those of the control group were injected(i.p.)with normal saline.Filiform acupuncture needle or moxibustion was applied to"Dazhui"(GV 14),unilateral"Geshu"(BL 17),"Shenshu"(BL 23)and"Zusanli"(ST 36)once daily for 3or 5days.Contents of serum IL-7and IL-18 were detected by ELISA.Results In comparison with the control group,the plasma leukocyte counts from day 2to day 5,the spleen index and serum IL-18 contents on day 3and5 after administration of CTX were significantly decreased,and serum IL-7contents on day 3and 5after CTX remarkably increased in the model group(P<0.05).Following the treatment,the spleen index on day 3and serum IL-18 content on day 5in the moxibustion group,serum IL-7and IL-18 contents on day 3and 5in the acupuncture group were significantly up-regulated(P<0.05).The effect of acupuncture was markedly superior to that of moxibustion in up-regulating serum IL-18 contents on day 3and 5after the treatment(P<0.05).No significant differences were found between the acupuncture and moxibustion groups in the leukocyte counts and spleen index after the treatment,and in serum IL-7contents on day 3(P>0.05).Conclusion Acupuncture and moxibustion can up-regulate the serum IL-7and IL-18 and white blood cell levels in immuno-suppression mice,which may contribute to their effects in relieving immune hypofunction caused by alkylating agent CTX.877|427|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)stimulation of different acupoints at the distal ends of the limbs on cardiac function in volunteers with acute hypoxia,so as to determine if its actions are realized by way of segmental innervations or meridians.Methods Twenty healthy volunteers were divided into control,Quze(PC 3),Shousanli(LI 10),Guangming(GB 37)and Zusanli(ST 36)groups(both PC 3and LI 10 are innervated by spinal C3-C6,and both ST 36 and GB 37 innervated by L5-S1).Acute hyoxia(simulating the conditions of about 5 000 mheight above the sea level)was induced by asking the volunteers to inhale low-oxygen gas mixture(10.8% O2+ 89.2% N2)for 30 min,when,the participants' cardiac output(CO),heart rate(HR),left cardiac work(LCW),left ventricular ejection time(LVET)were measured using a ICG Monitor and EA stimulation(10Hz/20 Hz,1-2V)was also conducted for 20 min following inhaling low-oxygen for 10 min.Results Before low-oxygen inhale,the levels of CO,HR,LCW and LVET ratios(test value/basic value)of the control,PC 3,LI 10,ST 36 and GB 37 groups were comparable(P>0.05).After inhaling low-oxygen gas mixture for 10 min,the levels of CO,HR,and LCW ratios were significantly increased,and the LVET ratios were notably decreased in the five groups(P<0.05).Compared with the 10min-low-oxygen inhale of the same one group,CO and HR ratios at both EA 10 min and 20 min in the PC 3and ST 36 groups,LCW ratios at EA 10 min in both PC 3and ST 36 groups were notably down-regulated(P<0.05),while the LVET ratios of both PC 3and ST 36 groups was significantly prolonged(P<0.05).No significant changes of CO,HR,LCW and LVET ratios were found in the LI 10 and GB 37 groups after EA for 10 min and 20min(P>0.05).Conclusion EA stimulation of Quze(PC 3)and Zusanli(ST 36),but not Shousanli(LI 10)and Guangming(GB 37)can lower CO,HR and LCW levels and increase LVET in volunteer subjects undergoing acute hypoxia,suggesting that the therapeutic effect of EA maybe not rely on the segmental innervations,but rather,depend on the meridians to which the acupoints belong.关键词：Acute hypoxia;Electroacupuncture;Left cardiac function;Acupoints at the distal limbs;Segmental innervations;Meridians777|184|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of"spleen-stomach needling"on changes of plasma malondialdehyde(MDA),protein carbonyl(PCO,an oxidative stress damage biomaker),8-hydroxyguanine(8-OHdG,an indicator of oxidative DNA damage)and the activity of superoxide dismutase(SOD)levels in patients with diabetic nephropathy,so as to explore its mechanisms underlying improving diabetic nephropathy.Methods Diabetic nephropathy patients were randomized into routine acupuncture(control)group(n=72)and spleen-stomach needling(observation)group(n=70)according to a random digits table and by using multi-centers,randomized and blind methods in the present study.All the patients were received routine medication.In addition,patients of the control group were treated by acupuncture stimulation of Shenshu(BL 23),Taixi(KI 3),Sanyinjiao(SP 6),Yanglingquan(GB 34),Xuanzhong(GB 39),Guanyuan(CV 4),Shousanli(Ll 10),Waiguan(TE 5),Yangxi(LI5),Liangqiu(ST 34),Shangjuxu(ST 37),Neiting(ST 44),Huaroumen(ST 24),and Dachangshu(BL 25),and those of the observation group treated by acupuncture stimulation of Zhongwan(CV 12),Quchi(LI 11),Hegu(LI 4),Xuehai(SP 10),Zusanli(ST 36),Yinlingquan(SP 9),Fenglong(ST 40),Diji(SP 8),Sanyinjiao(SP 6),Taichong(LR 3),Gaohuang(BL 43),Shenshu(BL 23),Baihuanshu(BL 30)and Zhongji(CV 3)with spleen-stomach needling method.The treatment of both groups was conducted twice a day for six weeks except Sundays.Plasma MDA,PCO and 8-OHdG contents were detected by using ELISA,and SOD detected using xanthine oxidase method.Results After 3weeks' treatment,plasma MDA,PCO and 8-OHdG contents were significantly increased in both control and observation groups,and plasma SOD was obviously reduced in both groups(P<0.05).No significant differences were found between the two groups in the plasma MDA,PCO and 8-OHdG contents(P>0.05).While after 6weeks' treatment,plasma MDA,PCO and 8-OHdG contents were significantly decreased(turning to the normal levels)and plasma SOD level in the observation group was significantly up-regulated(P<0.01).Following 3weeks' treatment,of the 72 and 70patients in the control and observation groups,10 and 20cases experienced marked improvement,22 and 15were improved,and 40 and 35invalid,respectively.After 6weeks' treatment,of the 72 and 70patients in the control and observation groups,12 and 39experienced marked improvement,28 and 20cases were improved,32 and 11invalid,respectively.The therapeutic effect of the observation group was obviously superior to that of the control group(P<0.01).ConclusionAcupuncture treatment with spleen-stomach regulation needling can down-regulate plasma MDA,PCO,8-OHdG contents and upregulate SOD activity in diabetic nephropathy patients,which may contribute to its effect in improving the patients' symptoms probably by resisting the oxidative stress damage.关键词：Diabetic nephropathy;Acupuncture therapy;Oxidative stress injury;Spleen-stomach regulation needling825|598|5更新时间：2023-08-11
- 摘要：Objective To observe the therapeutic effect of red-hot needle therapy combined with cupping for cervicogenic periarthritis of shoulder.Methods Forty-two cases of cervicogenic periarthritis of shoulder were randomized into red-hot needle group and routine acupuncture group(n=21).For patients of the routine acupuncture group,the filiform needles were applied to Tianzhu(BL 10),Jianjing(GB 21),Jianzhongshu(SI 15),Jianzhen(SI 9),Jianliao(TE 14),Jianyu(LI 15),Jianqian,Tianzong(SI 11)and Ashi-points on the affected side,followed by conducting cupping at the anterior and posterior regions of the affected shoulder,SI 11,GB 21 and SI 15.For patients of the red-hot needle group,the Ashi-points on the affected shoulder were punctured with cauterized filiform needles,following by performing cupping.The treatment was performed once daily and once every other day respectively for two weeks.The shoulder motor function was assessed according to the adjusted Constant-Murley test.Results After the treatment,the integrated scores of shoulder pain,shoulder-joint activities in daily living and shoulderjoint motion range were significantly increased in both groups compared with pre-treatment in the same one group(P<0.05)and obviously higher in the red-hot needle group than in the routine acupuncture group(P<0.05).Of the two 21 cases of shoulder periarthritis patients in the routine acupuncture and red-hot needle groups,4and 8were cured,14 and 12experienced improvement,3and 1was invalid,with the effective rates being 85.71% and 95.24%,respectively.The therapeutic effect of the redhot needle therapy was significantly superior to that of the routine acupuncture(P<0.05).Conclusion The red-hot needle therapy combined with cupping is effective in relieving cervicogenic shoulder periarthritis and is remarkably superior to routine acupuncture combined with cupping in improving shoulder periarthritis patients' symptoms.关键词：Cervicogenic periarthritis of shoulder;Red-hot needle;Cupping;Adjusted Constant-Murley score769|1043|5更新时间：2023-08-11
- 摘要：Objective To observe clinical effects of"Santong needling"by stages for patients with moderate and severe peripheral facial paralysis(PFP)in the acute period.Methods Sixty patients with moderate and severe PFP were equally and randomly divided into Santong needling group and control group.The patients of the Santong needling group were treated by shallow puncture of Yifeng(TE 17,on the affected side)and Hegu(LI 4,on the healthy side)without needle manipulation in the acute stage("Qing-tong",mild dredging),by oblique-penetrative puncture of Dicang(ST 4)to Jiache(ST 6),etc.,and moderate manipulation of needles in the resting stage("Qiang-tong",stronger dredging),and by oblique-penetrative puncture of Dicang(ST 4)to Jiache(ST 6),etc.,and reinforcing needling manipulation in the recovery stage("Bu-tong",tonifying dredging).The patients of the control group were treated by"Jingjin"(musculotendon)puncture of the same acupoints.The treatment was conducted once daily,5times per week for 6weeks.The 0-3-grade scores of therapeutic effects were evaluated according to the severity of the patients' symptoms and signs(including forehead stripes,palpebral fissure,nasolabial groove,drooping of the mouth angle,bulging cheek-air leak,food residue,dysgeusis,hyperacusi,lacrimation,and orbicularis oculi reflex).The therapeutic effect index=(score of pre-treatment-score of post-treatment)/score of pre-treatment x100%.The patients' life quality scores were assessed by using the World Health Organization Quality of Life(WHOQOL)-BREF questionnaire.Results After the treatment,the scores of clinical symptoms and signs were significantly decreased in both control and Santong needling groups in comparison with pre-treatment in the same one group(P<0.05),suggesting an improvement of facial nerve function after 6weeks' treatment.Of the two 30 patients in the control group and Santong needling group,11(36.7%)and 15(50.0%)were cured,8(26.7%)and 9(30.0%)experienced marked improvement,5(16.6%)and 4(13.3%)were effective,and 6(20.0%)and 2(6.7%)invalid,with the effective rates being 80.0% and 93.3%,respectively.The therapeutic effect of Santong needling group was evidently superior to that of the control group(P<0.05).Three-months' follow up showed that the WHOQOL-BREF scores were comparable between pre-and post-treatment in the same one group and between the two groups(P>0.05).Conclusion Santong needling by stages is effective in improving clinical symptoms and signs of patients with moderate and severe peripheral facial paralysis.关键词：Peripheral facial paralysis;Acupuncture therapy;"Santong Needling"by stages;Therapeutic effects;Randomized controlled trial785|503|4更新时间：2023-08-11
- 摘要：The design of"sham acupuncture"is necessary in clinical trials of acupuncture for confirming its effectiveness in the treatment of different disorders.The authors of the present paper made a comprehensive analysis on"sham acupuncture"or"placebo acupuncture"in clinical trials of acupuncture therapy in western countries from 1)the definition and background of sham acupuncture,2)current state of acupuncture clinical trials with sham acupuncture design in different countries,3)current situations of acupuncture clinical trials with sham acupuncture design in Germany,and 4)current state of large sample,randomized controlled clinical trials of acupuncture therapy with sham acupuncture design in other western countries.The authors of the present paper also make an analysis on the developing trends of acupuncture clinical trials with sham acupuncture design and put forward some problems existing in current acupuncture researches.关键词：Acupuncture therapy;Sham acupuncture;Randomized controlled clinical trial;Western countries;Literature study1100|1094|6更新时间：2023-08-11
- 摘要：Outlet obstruction constipation(OOC)is a common clinical problem affecting many people's daily life quality nowadays.Acupuncture therapy is effective in easing OOC to reduce patients' sufferings by stimulation of Baliao-points i.e.,bilateral Shangliao(BL 31),Ciliao(BL 32),Zhongliao(BL 33)and Xialiao(BL 34),which has been confirmed repeatedly in clinical practice.In the present paper,the authors reviewed development of studies on the underlying mechanisms of acupuncture stimulation of Baliao-points for OOC from:1)lowering the sensitivity of intraganglionic laminar nerve endings(rIGLEs)to rectal mechanical distension during defecation,2)raising the level of circulating cholecystokinin(CCK)to improve the sensory threshold of the rectum,3)lowering the excitability ofγ-motor neurons in the spinal anterior horns controlling the slow contraction of the rectal sphincter muscle,4)inhibiting the excitability of sympathetic output from the spinal lateral horns,5)easing the tonic contraction of the pelvic muscles to reduce the release of 5-hydroxy tryptamine(5-HT),histamine,and other related chemical mediators due to local ischemia.Moreover,further study on the mechanism of acupuncture stimulation of Baliao-points underlying improving OOC may help up find more specific and effective therapeutic targets and provide more reliable experimental foundation and theoretical basis.关键词：Baliao-points;Outlet obstruction constipation;Rectal hyposensitivity;Pelvic floor muscle spasm784|954|4更新时间：2023-08-11
- Postal code：100700
- Tel：010-64089344,84014607 Email：zcyjbjb@vip.163.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05017590号-8
京公网安备11010802020460号 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0