最新刊期
卷 43 ， 期 7 ， 2018
- 摘要：Objective To compare the therapeutic effect of electroacupuncture(EA)of"Shenmen"(HT 7,Yuan point of the Heart Meridian),"Zhizheng"(SI 7,Luo point of the Small Intestine Meridian)and"Xinshu"(BL15,Back-shu point)on the expression of nerve growth factor(NGF)and its receptor,tyrosine kinase A(TrkA)in the cerebral cortex of myocardial ischemia(MI)rats.Methods A total of 70 male SD rats were randomized into sham operation(sham,n=10),model,HT 7,SI 7,and BL15 groups(n=15 in each of the latter 4 groups).The MI model was established by ligation of the anterior descending branch of the left coronary artery.EA(1 mA,2 Hz)was respectively applied to HT 7,SI 7 and BL15 for 15 min,once per day for 1 week.Immunohistochemistry and real-time fluorescent quantitative PCR were used to detect the expression of NGF and TrkA proteins and genes in the cerebral cortex.Results Compared with the sham group,no significant changes were found in the number of NGF immune-reaction(IR)-positive and TrkA IR-positive cells,and the expression levels of NGF mRNA and TrkA mRNA in the model group(P>0.05).After EA intervention,the number of NGF and TrkA IR-positive cells and the expression of NGF mRNA and TrkA mRNA in each of the 3 EA groups were significantly increased relevant to the model group(P<0.01,P<0.05).The effect of EA at BL 15 was significantly superior to that of EA at HT 7 and SI 7 in increasing the number of NGF and TrkA positive cells and up-regulating the expression of their mRNAs(P<0.01,P<0.05).Conclusion EA at HT 7,SI 7 and BL 15 can increase the levels of expression of NGF and TrkA proteins and mRNAs in the cerebral cortex of subacute MI rats and the effects of EA-BL 15 are obviously superior to those of EA-HT 7 and EA-SI 7,suggesting a relative specificity of the effect of EA at different acupoints.关键词：Acute myocardial ischemia;Cerebral cortex;Nerve growth factor;Tyrosine kinase A;Relative specificity of acupoint649|441|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)stimulation of"Shenmen"(HT 7)-"Tongli"(HT 5)segment of the Heart Meridian on neuronal electrical activities of hypothalamic paraventricular nucleus(PVN)in rats with myocardial ischemia(MI),so as to investigate its possible mechanism underlying improvement of MI.Methods Thirty-two SD rats were randomly divided into sham control,model,HT 7-HT 5 and"Taiyuan"(LU 9)-"Lieque"(LU 7)groups(n=8 in each group).EA preconditioning(2 Hz,1 V,20 min)was applied to bilateral HT 7-HT 5 and bilateral LU 9-LU 7,respectively,once everyday for 7 days.The electrical activities of the right PVN region were recorded by the implanted microelectrode array(2×4)and Plexon multi-channel acquisition system.Cluster analysis of neuronal signals was carried out by Offline Sorter software.The discharge waveforms,autocorrelation and cross-correlation of neuronal activities were analyzed by using Neuro Explorer software.Results Cluster analysis of neuronal signals showed that 2,2,1 and 1 interneuron in the sham,model,HT 7-HT 5,and LU 9-LU 7 groups,and 3 pyramidal neurons in the HT 7-HT 5 were acquired.Cross correlation analysis showed that the SPK 02 aand SPK 02 bneurons of the HT 7-HT 5 group had an inhibitory relationship.The total discharge frequency was significantly increased in the model group relevant to the sham group(P<0.01),and was markedly lower in the HT 7-HT 5 group than in the model group and LU 9-LU 7 group(P<0.01).Real-time spectrum analysis showed that the local field potential spectrum energy of the HT 7-HT 5 group was significantly lower than that of the model group and the LU 9-LU 7 group.Conclusion EA of HT 7-HT 5 segment of the Heart Meridian can inhibit the electrical activity of interneuron and activate the electrical activity of pyramidal neuron in PVN region,and an inhibitory relationship exists between the interneuron and pyramidal neuron in MI rats,which may be a mechanism of EA in regulating activities of the ischemic heart.关键词：Electroacupuncture;Heart meridian;Myocardial ischemia;Hypothalamic paraventricular nucleus;Neuronal activity;Microelectrode array613|621|11更新时间：2023-08-11
- 摘要：Objective To explore the neuroanatomical basis of acupoint-visceral correlation by studying the distribution of the neurons associated with acupoint"Shenshu"(BL 23)area and adrenal gland in rats.Methods AF 488-CTB and AF 594-CTB were injected into the left side of BL 23 area and adrenal gland in the same rat respectively.Three days after injection,the dorsal root ganglions(DRG),sympathetic chain,and spinal cord were dissected out from the perfused rats.The neuronal labeling with AF 488/594-CTB was directly observed on the sections under a fluorescent microscope or a laser scanning confocal microscope.Results All neural labeling was observed in the injection side.The sensory neurons associated with both acupoint BL 23 and adrenal gland distributed from thoracic(T)10 to lumbar(L)2 DRG with high concentration in T 12-T 13 and T 11-T 12,respectively,in which some of them were simultaneously labeled with both AF 488/594-CTB and located in T 12-L 1 DRG.For the sympathetic innervation,the postganglionic neurons correlated with BL 23 and adrenal gland were labeled with AF 488/594-CTB separately in the sympathetic chain at the lumbar segments,while the labeled preganglionic neurons were only observed at the lateral horn of T 11-T 13 spinal segments in the cases of adrenal gland.In addition,the labeled motor neurons were mainly detected in the spinal ventral horn at cervical(C)7-C 8 and T 11-L 1 segments.Conclusion These results indicate that there are segmental correlation between BL 23 and adrenal gland on the sensory and sympathetic innervations,and this correlation might be a neural pathway for modulating the function role of adrenal gland through BL 23 needling.关键词：Adrenal gland;Shenshu(BL 23);Dual fluorescent neural tracing technique;Sensory neuron;Sympathetic preganglionic neuron;Sympathetic postganglionic neuron;Motor neuron961|415|8更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)at"Tianshu"(ST 25)on visceral pain and colonic mast cell(MCs)number and tryptase and SP expression in post-infectious irritable bowel syndrome(PI-IBS)rats,so as to reveal its mechanisms underlying improvement of PI-IBS.Methods Forty-five female Wistar rats were randomly divided into control,model and EA groups(15 rats/group,3 rats/group used for H.E.staining,and 12 rats/group for immunohistochemistry).The PI-IBS model was established by intra-anal injection of mixed liquor of 50% ethyl alcohol and trinitro-benzene-sulfonic acid(TNBS).EA(2 Hz/15 Hz,0.5-1.0 mA)was applied to bilateral"Tianshu"(ST 25)for 30 min,once every day for 14 days.The visceral pain was measured by abdominal withdrawal reflex(AWR),for which the rectal implanted air balloon was dilated by infusion of normal saline.The histopathological changes of the colon tissue were observed after H.E.staining,and the colonic MCs were displayed by Toluidine blue staining.The expression of tryptase and SP proteins in the colon specimens were detected by immunohistochemistry.Results The AWR threshold was significantly lowered in the model group relevant to the control group(P<0.05)and considerably increased after EA intervention in comparison with the model group(P<0.05).The number of MCs and the expression levels of colonic tryptase and SP proteins in the colon tissues were significantly higher in the model group than in the control group(P<0.05),and obviously lower after EA intervention in the EA group than in the model group(P<0.05).Conclusion EA of"Tianshu"(ST 25)can inhibit visceral pain in PI-IBS rats,which may be associated with its effects in activating MCs and down-regulating the expression of tryptase and SP proteins in the colonic tissues.关键词：Electroacupuncture;Post-infectious irritable bowel syndrome;Visceral pain;Colonic mast cells;Tryptase;Substance P623|813|5更新时间：2023-08-11
- 摘要：The meridians and Zangfu-organs(viscera)are two major systems in the basic theory of traditional Chinese medicine(TCM).They were primarily emerged together in simple form possibly in the Han Dynasty shown by the unearthed silk manuscripts of Changsha Mawangdui Han Tomb,and became a systematic theory in classical book "Neijing"(The Yellow Emperor's Internal Classic).After classification of the meridians' disease manifestations described in book "Ling Shu-Jing Mai"(Miraculous Pivot:Meridians)we found that more than half of the manifestations appear along the running course of meridians.The symptoms and signs of disorders of the meridians correlated with viscera which have the same name in western medicine account for approximately 10%,and the rest pathologic manifestations correlated with the viscera in terms of TCM are approximately 30%.Current researches about meridian and viscera mainly focus on viscera,particularly on the stomach and heart.The researches on meridians and viscera that use real-time effects of acupuncture as indicators have a complicated relationship with the nerve segments,which presented as that"multiple meridians control one viscus"and the specificity of meridian.Our chronic experiment about blocking the meridians suggested an association between meridian and pathologic manifestations of viscera.Physical index measurement and mathematical analysis can verify certain patterns of meridian-physical appearances,which would be a direction for future meridian research.The stabilities of visceral functions rely on negative feedback regulation,which are probably realized by way of somatic and visceral afferent and efferent nerves and volume transmission of the tissue fluid channels of meridians.Therefore,signals of acupuncture stimulation of the distant acupoints might be transmitted to the central nervous system through neurohumor relay,open of the "negative feedback channels of meridians",achieving a bidirectional regulation,and at the same time,producing aphenomenon of"sensation propagated along the meridian"during signal transmission.关键词：Meridian;Correlations between meridians and viscera;Viscera;Stability;Volume transmission691|994|9更新时间：2023-08-11
- 摘要：The correlation between meridians and viscera is the key content of the meridian theory in acupuncture medicine.This paper introduces the close relationship between the meridians running on the body surface and viscera from ancient literature,modern experimental studies(such as acupoint sensitization,neuro-endocrine-immune networks,etc.),and clinical application in the treatment of various conditions with acupuncture,moxibustion,Guasha(scraping)therapies,etc.We also presented some related research ideas(i.e.,taking the clinical treatment as the forerunner,paying more attention to multi-disciplinary integration,combining clinical and basic research together,doing research from tridimensional point of review,and making full use of advanced technology,etc.)in order to provide a possibly favorable help for researchers to further reveal the scientific mechanisms underlying correlation between the meridian and viscera in the future.关键词：Correlations between meridians and viscera;Acupuncture;Research ideas791|759|3更新时间：2023-08-11
- 摘要：The theory that meridians connect the upper and lower,interior and exterior of the whole human body comes from the clinical experience of acupuncture therapy mainly by choosing the acupoints of 12 regular meridians at the four limbs.During the process of merging the theories of meridians and Zangfu-organs(viscera),the expression of the intrinsic relationship of such connection gradually becomes more and more complicated.Therefore,the study on the correlation between the meridian and viscus should start from the characteristics of the meridian theory itself,and discriminate the"reasonable and unreasonable"components of the related theoretical descriptions in the literature in order to explore the scientific values of this correlation.Based on this viewpoint,the present article makes a brief analysis on this proposition from the following two angles of view:the understanding of such proposition and the historical evolution of the meridian theory.关键词：Correlations between meridians and viscera;Meridian;Acupoint indications511|669|4更新时间：2023-08-11
- 摘要：Objective The present study aimed at observing the profile of metabolites in sensitized acupoints induced by myocardial ischemia(MI)and the effect of acupuncture intervention on the changes of the metabolites so as to explore the material basis of acupoint sensitization.Methods A total of 20 New Zealand rabbits were randomly and equally divided into a control group and a model group.The MI model was established by occlusion of the anterior descending branch of the left coronary artery with a controllable air balloon inflation method for 5 min/time,twice a day(4-hours' interval)for continuous 5 days(the first stage of MI).After one day's rest,another 5 days' occlusion was conducted again(the second stage of MI)in the same way.The rabbits of the control group were treated with the same procedures but without occlusion.Subcutaneous microdialysis fluid samples were collected from "Neiguan"(PC 6),"Shenmen"(HT 7),"Xinshu"(BL 15),and "Taixi"(KI 3)regions on day 8(after recovery from operation),14(the first stage of MI),and 20(the second stage of MI),as well as collected from PC 6 region during and post-acupuncture stimulation of PC 6,respectively.Manual acupuncture stimulation was applied to the right PC 6 for 30 min.Partial least squares-linear discriminant analysis(PLS-DA)was used to identify different metabolism patterns of the microdialysis fluid sample between groups and at different time-points in the same one group,and the distinct metabolites as the potential markers between groups were weighted via the values of variable importance in the projection(VIP)in combination with t-test analysis.An area under the curve(AUC)>1.0 indicated a test exhibiting good discrimination between groups.Results Six metabolites identified to be significantly different between the control and model groups were L-glutamic acid,phenylalanine and3-hydroxyisobutyric acid(which were significantly increased relevant to the control group),and L-histidine,octadecanedioic acid and 9-keto palmitic acid(significantly decreased relevant to the control group)in the microdialysate of PC 6,HT 7 and BL 15 regions.In the microdialysate of PC 6,4 metabolites including L-glutamic acid,octadecanedioic acid and 8-isohydroxy PGF 2α(significantly increased),as well as L-histidine(markedly decreased)were identified to be considerably different between the model and control groups.After acupuncture for 30 min,the AUC level of L-glutamic acid was further significantly increased(P<0.05),that of L-histidine obviously decreased,and those of octadecanedioic acid and 8-isohydroxy PGF 2αturned back nearly to the level of pre-MI.Conclusion L-glutamic acid,phenylalanine,3-hydroxyisobutyric acid,L-histidine,octadecanedioic acid and 9-keto palmitic acid from PC 6,HT 7 and BL 15 regions may be used as the material biomarker for MI-induced sensitization of these acupoints.Manual acupuncture intervention of PC 6 induces a significant change of L-histidine and L-glutamic acid in the local subcutaneous tissues.关键词：Myocardial ischemia;Sensitization of acupoints;Subcutaneous metabolites;Manual acupuncture stimulation701|549|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of liver-soothing and mental-activity-regulating(LSMAR)needling on the expression of receptor activity-modifying protein 1(RAMP 1,receptor of calcitonin gene-related peptide),5-hydroxytryptamine1 Dreceptor(5-HT 1 DR)in the spinal trigeminal nucleus(STN)and midbrain in migraine rats,so as to explore its underlying mechanism in relieving migraine.Methods A total of 40 male Wistar rats were randomly divided into control,model,LSMAR and conventional needling groups(n=10 rats in each).The migraine model was established by subcutaneous injection of nitroglycerin at the posterior neck.LSMAR was applied to"Baihui"(GV 20),bilateral"Fengchi"(GB 20),"Neiguan"(PC 6)and"Taichong"(LR 3)in the LSMAR group and conventional needling was applied to"Baihui"(GV 20)and bilateral"Fengchi"(GB 20)in the conventional acupuncture group for 30 min,once a day for 8 days before modeling.The expression levels of RAMP 1 and 5-HT 1 DR proteins and mRNAs in the STN and mesencephalon were detected by real-time fluorescent quantitative PCR and Western blot,separately.Results Compared with the control group,the expression levels of RAMP 1 protein and mRNA in STN and mesencephalon were significantly increased(P<0.05)and those of 5-HT 1 DR protein and mRNA considerably decreased(P<0.05)in the model group.After the acupuncture treatment,the increased levels of RAMP 1 protein and mRNA and the decreased levels of 5-HT 1 DR protein and mRNA in the STN and midbrain were obviously reversed in the LSMAR and conventional needling groups relevant to the model group(P<0.05).The effect of LSMAR needling was significantly superior to that of conventional needling in down-regulating the expression levels of RAMP 1 mRNA and protein in the STN and mesencephalon(P<0.05)and in up-regulating the expression levels of 5-HT 1 DR mRNA and protein in the two brain regions(P<0.05).Conclusion Manual acupuncture stimulation of GV 20,GB 20,etc.can inhibit the expression of RAMP 1 protein and mRNA in the STN and midbrain,and up-regulate the expression of 5-HT 1 DR in the two brain regions of migraine rats,which may be related to its effect in relieving migraine.关键词：Acupuncture;Liver-soothing and mental-activity-regulating needling;Migraine;Receptor activity-modifying protein 1;5-HT 1D receptor717|502|9更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA)of"Jiaji"(EX-B 2)on limb locomotor function and expression of Ras homolog gene family member A(RhoA),Rho-associated kinase Ⅱ(ROCK Ⅱ)and myosin light chain(MLC)proteins in the anterior horn of spinal cord in acute spinal cord injury(ASCI)rats,so as to explore its mechanisms underlying improvement of SCI-induced limb locomotor dysfunction.Methods Forty-eight female Wistar rats were randomly divided into sham operation(sham),ASCI model(model),EA EX-B 2(EA)and ROCK inhibitor(Fasudil)groups which were further divided into 14 dand 28 dsubgroups(n=6 in each).The ASCI model was made by using weight drop striking method.Three hours after modeling,EA(100 Hz,0.4,0.6 mA)was applied to EX-B 2(T 9,T 11)for 30 min,once daily for 14 dand 28 d,respectively.The ROCK inhibitor(hydrochloride Fasudil,10 mg/kg)was administrated by intraperitoneal injection immediately after modeling,once a day,continuously for 14 dor 28 d.The expression of RhoA,ROCK Ⅱ and MLC proteins in the spinal cord anterior horn tissue(T 10)was detected by immunohistochemistry.The rats' hindlimb locomotor function was assessed according to Basso,Beattie and Bresnahan(BBB)locomotor rating scale(21-points).Results After ASCI,the BBB scores were significantly lower in the model group than in the sham group on day 14 and 28(P<0.05),and obviously higher in the EA and inhibitor groups than in the model group(P<0.05),suggesting an improvement of the hindlimb locomotor function after EA intervention or suppression of ROCK.Immunohistochemical results indicated that the numbers of RhoA,ROCK Ⅱ and MLC immune-reaction positive cells in the anterior horn of spinal cord were significantly more in the model group than in the sham group(P<0.05),and remarkably decreased in both EA and inhibitor groups on day 14 and 28 relevant to the model group(P<0.05).The therapeutic effects of EA were markedly weaker than those of inhibitor Fasudil in up-regulating BBB score and down-regulating the number of RhoA,ROCKⅡ and MLC positive cells(P<0.05).Conclusion EA of EX-B 2 can improve the hindlimb locomotor function in ASCI rats,which may be associated with its effect in down-regulating the expression of RhoA,ROCK Ⅱ and MLC proteins(i.e.,inhibiting the RhoA/ROCK signaling pathway)in the anterior horn of spinal cord.关键词：Acute spinal cord injury;Electroacupuncture;Myelin-associated inhibitory factors;Rho/ROCK Ⅱ signaling pathway;RhoA;ROCK Ⅱ;MLC647|589|10更新时间：2023-08-11
- 摘要：Objective To evaluate the clinical effect of herbal cake-partitioned moxibustion combined with medication in the treatment of moderate and severe persistent allergic rhinitis(PAR)patients of spleen deficiency pattern.Methods Sixty patients with moderate and severe PAR of spleen deficiency pattern were randomized into medication(Rhinocort)group and herbal cake-partitioned moxibustion combined with medication group(combination group,n=30 cases in each).Medicinal cake-partitioned moxibustion was applied to Yintang(GV 29,for about 20 min),Shenque(CV 8,about 50 min),bilateral Zusanli(ST 36,about 40 min)and Hegu(LI 4,about 40 min)once every other day for successive 8 weeks.All the patients received treatment with Rhinocort nasal spray,2 jets/day for each nostril(64 !g/jet)for 8 weeks.The patients' conditions(symptoms of nasal sufferings,sleeping,sniffle,eye,emotion,etc.)were assessed by visual analogue scale(VAS)and rhino conjunctivitis quality of life questionnaire(RQLQ,24 items of 7 aspects,0-6 points/item),respectively.The spleen deficiency syndrome score was determined according to"Guiding Principles for Clinical Research of New Drugs of Chinese Materia Medica"(2002).The therapeutic effect was assessed by referring to"the Diagnostic and Therapeutic Principles and Recommended Schemes for Allergic Rhinitis"(2004).Results Following the treatment,the scores of VAS,RQLQ,spleen deficiency syndrome were significantly decreased on the 4 thand the 8 th week of treatment in both groups in comparison with those of their own individual pre-treatment(P<0.05,P<0.01).Four weeks' follow-up survey showed that the VAS score and RQLQ score of the combination group were obviously lower than those of the medication groups(P<0.05),suggesting a better post-effect of moxibustion.Of the two 30 cases in the medication and combination groups,9 and 9 experienced marked improvement in their symptoms,16 and 17 were effective,and5 and 4 ineffective,with the effective rate being 83.3% and 86.7%,respectively.No significant differences were found between the two groups in the VAS and RQLQ scores on the 4 th and 8 th week during treatment and in the effective rate(P>0.05).Conclusion Herbal cake-partitioned moxibustion combined with hormone(Rhinocort)nasal spray is effective in relieving symptoms of moderate and severe PAR patients of spleen deficiency syndrome,and has a better post-effect.关键词：Allergic rhinitis;Herbal cake-partitioned moxibustion;Combined treatment;Randomized controlled trial(RCT)828|472|4更新时间：2023-08-11
- 摘要：Objective To observe the effectiveness of electroacupuncture(EA)therapy in promoting medicinal abortion.Methods Sixty participants with early pregnancy were randomly divided into medication(control)and EA+medication groups(n=30 in each).Participants in the control group were treated by regular medication(mifepristone and misoprostol tablets),and those of the EA group were treated by EA stimulation(2 Hz/100 Hz,a tolerable strength)of bilateral Hegu(LI 4)and Sanyinjiao(SP 6)for 25 min,and also by the same medication method mentioned in the control group.Participants of both groups were admitted to the hospital for observation on the 3 rd day after taking medication.The abdominal pain was assessed by visual analogue scale(VAS)score,and the foetal sac discharge duration and the complete abortion rate were recorded.Results After the treatment,the VAS scores of both groups were significantly reduced in comparison with their own pre-treatment in each group(P<0.01),and the VAS score of the EA+medication group was significantly lower than that of the medication group(P<0.01).The foetal sac discharge duration was significantly shorter in the EA+medication group than in the medication group(P<0.01).The abortion rates showed no significant difference between the two groups(P>0.05).Conclusion EA stimulation of Hegu(LI 4)and Sanyinjiao(SP 6)has a positive effect in assisting medicinal abortion in reducing abdominal pain and shortening foetal sac discharge duration.关键词：Medical abortion;Electroacupuncture;Hegu(LI 4);Sanyinjiao(SP 6)637|370|4更新时间：2023-08-11
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