最新刊期
卷 44 ， 期 1 ， 2019
- 摘要：Objective To observe the effect of herbal cake-partitioned moxibustion(Moxi)on tumor necrosis factor(TNF)-α/TNF receptor 1(TNFR1)-associated death domain(TRADD)/Fas-associated death domain(FADD)pathway-mediated apoptosis of intestinal epithelial cells in Crohn's disease(CD)rats,so as to explore its underlying mechanisms in the treatment of CD.Methods Forty-eight SD male rats were randomly divided into normal,model,Moxi and medication groups(n=12 rats in each).The CD model was established by intra-annual perfusion of 2,4,6-trinitrobenzene sulfonic acid(TNBS)solution(TNBS∶50% alcohol=2∶1,3 mL/kg),once every 7 days,4 times altogether.For rats of the Moxi group,moxibustion was given to"Tianshu"(ST25)and"Qihai"(CV6),two moxa-cones every time,once daily for 10 days.For rats of the medication group,intragastric perfusion of mesalazine solution was given twice daily for 10 days.After the treatment,the colonic epithelium tissue was sampled.The epithelial cells were purified and cultured to establish an in vitro intestinal epithelial barrier,and added with TNF-α(apro-inflammatory factor,100 ng/mL)in the culture medium for 24 hfor making an increased epithelial permeability model.The permeability of intestinal epithelial cell barrier was evaluated by detecting the fluorescence yellow transmittance of the TNF-α-incubated cell medium.Western blot was used to detect the expression levels of TNFR1,TRADD,receptor-interacting protein 1(RIP1),FADD and zinc finger protein A20(A20,a ubiquitination enzyme for inhibiting activation of TRADD and RIP1)of the cultured intestinal epithelium cells.The apoptosis of the TNF-α-incubated intestinal epithelial cells was detected by flow cytometry.Results After modeling and compared with the normal group,the fluorescence yellow transmittance of intestinal epithelia cells,apoptosis rate,and expression levels of TNFR1,TRADD,and RIP1 proteins were significantly increased(P<0.001,P<0.01),and the expression of A20 was significantly decreased(P<0.01)in the model group.In comparison with the model group,the fluorescence yellow transmittance of intestinal epithelial cells,the apoptosis rate and expression levels of TRADD,RIP1 and FADD were remarkably down-regulated(P<0.001,P<0.01),and the expression of A20 was significantly up-regulated(P<0.01)in both the Moxi and medication groups.Conclusion Herbal cake-partitioned moxibustion may down-regulate the permeability of intestinal epithelial barrier and the apoptosis of intestinal epithelial cells by way of suppressing TNF-α-mediated cellular apoptosis pathway of intestinal epithelium in CD rats.关键词：Herbal cake-partitioned moxibustion;Crohn's disease;Tumor necrosis factor-α;Cell apoptosis pathway598|552|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of eletroacupuncture(EA)intervention on lipid metabolism and expression of AMP-activated kinase(AMPK),p38 mitogen-activated protein kinase(p38 MAPK)and peroxisome proliferator-activated receptorγ(PPARγ)protein in the liver in rats with insulin resistance(IR),so as to reveal its mechanisms underlying improvement of IR.Methods Forty male SD rats were randomly divided into blank control,model,medication,and EA groups(n=8 in each).The IR model was established by feeding the rats with high-fat diet for 12 weeks.After successful establishment of model,the rats in the blank control group and model group were fixed in the self-made rat bag without receiving any treatment.The rats in the medication group were treated by gavage of pioglitazone(10 mL/kg).EA(2 Hz/100 Hz,1 mA)was applied to bilateral"Fenglong"(ST40)and"Sanyinjiao"(SP6)for 20 min,once a day,for continuous 14 days for rats in the EA group.The ultrastructure of the liver tissue was observed by transmission electron microscope(TEM).Blood samples were taken from the abdominal aorta for detecting serum C-peptide(C-P),adiponectin(ADP),leptin(LEP)and resistin(RES)contents using enzyme linked immunosorbent assay(ELISA).The expression levels of AMPK,p38 MAPK and PPARγproteins in the liver tissue were detected by Western blot.Results After modeling,the contents of serum C-P,LEP and RES,and the expression of liver p38 MAPK protein were significantly up-regulated(P<0.01,P<0.05),and the content of ADP and expression of AMPK and PPARγsignificantly down-regulated in the model group compared with the blank control group(P<0.01).The increased contents of C-P,LEP and RES,and p38 MAPK protein expression and the decreased serum ADP and hepatic AMPK and PPARγexpression levels were completely reversed in both the EA and medication groups relevant to the model group(P<0.01,P<0.05).No significant differences were found between the EA and medication groups in up-regulating the levels of ADP,AMPK and PPARγand in downregulating the levels of C-P,LEP,RES and p38 MAPK(P>0.05).Outcomes of TEM showed that morphological structure of liver mitochondria was damaged,including a large number of lipid droplets,being blur in appearance,rupture of partial membrane,dissapearance of partial mitochondrial crests with vacuolus-like appearance and decrease of rough endoplasmic reticulum in the model group,which was relatively milder in both EA and medication groups.Conclusion EA intervention is able to improve the disorder of lipid metabolism of IR rats,which may be associated with its effects in lowering the activity of fatty acid synthesis-related enzymes and regulating AMPK/p38 MAPK/PPARγsignaling to improve IR in the liver tissue.关键词：Eletroacupuncture;Insulin resistance;AMP-activated protein kinase;p38 mitogen-activated protein kinase;Peroxisome proliferator-activated receptorγ550|364|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of eye-acupuncture intervention on cerebro-cortical apoptosis of microvascular endothelial cells,neurons and astrocytes(main components of neurovascular unit)and the expression of Bad(an apoptosis promoter)and B-cell lymphoma-extra large(Bcl-xL)proteins in focal cerebral ischemia-reperfusion injury(CIRI)rats,so as to explore its mechanisms underlying improvement of CIRI.Methods SD rats were randomly divided into control,sham-operation,model 3 h,model 24 h,model 72 h,eye-acupuncture 3 h,eye-acupuncture 24 hand eye-acupuncture 72 hgroups(n=12 in each group).The CIRI model was established by middle cerebral artery occlusion/reperfusion(MCAO/R).Eye-acupuncture was applied to bilateral"Gan"(Liver)regions,"Shen"(Kidney)regions,"Shangjiao"(Upper-energizer)and"Xiajiao"(Lower-energizer)for 20 min,once 3 hand every 12 hafter modeling.The expression levels of Bad and Bcl-xL in the ischemic cerebral cortex tissue were detected by Western blot.The apoptotic neurons,microvascular endothelial cells and astrocytes were assayed by immunofluorescence double labeling(Nestin/TUNEL,CD34/TUNEL and glial fibrillary acidic protein[GFAP]/TUNEL)separately.Results After modeling,the numbers of apoptotic neurons,microvascular endothelial cells and astrocytes in the ischemic cerebral cortex tissue were significantly increased in the model 72 hgroup than in the sham-operation group(P<0.01).Following the treatment,the numbers of the 3 types of apoptotic cells were markedly lower in the eye-acupuncture 72 hgroup than in the model72 hgroup(P<0.01).The expression levels of Bad and Bcl-xL proteins were notably up-regulated in the model 3 h,model 24 h and model 72 hgroups than in the sham operation group(P<0.01).Following eye-acupuncture intervention,modeling induced increase of the Bad expression were obviously reversed in eye-acupuncture 24 hand eye-acupuncture 72 hgroups than those in the2 model groups(P<0.05).And the increase of Bcl-xL expression levels were further increased in the eye-acupuncture groups in comparison with those in the 3 model groups(P<0.01).Conclusion Eye-acupuncture can down-regulate the expression of Bad protein,and up-regulate the expression of Bcl-xL protein in the ischemic cerebral cortex in CIRI rats,which may contribute to its function in reducing apoptotic neurons,microvascular endothelial cells and astrocytes,suggesting aprotective effect of eye-acupuncture intervention on neurovascular unit.关键词：Cerebral ischemia-reperfusion;Eye-acupuncture;Apoptosis;Neurovascular unit;Bad;Bcl-xL543|445|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture on activities of microglia in traumatic brain injury(TBI)rats.Methods Fifty-four male SD rats were randomly and equally divided into normal control,model and acupuncture groups according to the random number table(n=18 rats in each group).The TBI model was established by using a free fall brain injury striking device after exposing the local cranial bone(to induce the left parietal cerebral contusion).Acupoints"Baihui"(GV20),"Shuigou"(GV26),"Fengfu"(GV16),"Yamen"(GV15)and bilateral"Hegu"(LII4)were stimulated intensively by twirling the filiform needles with force at a range of>360°and a frequency of 160-180 cycles/min for 10 sec in every acupoint,once every5 min during the 15 minutes' needle retaining.The treatment was given once every day for successive 14 days.The rats of the normal and model groups were grabbed and fixed with the same procedure.The behavioral changes were tested using modified neurological severity score(mNSS).The histopathological changes of the injured cerebral cortex tissues were observed by using hematoxylin-eosin(H.E.)staining,and the fluorescence intensity of Iba-1(marker of microglia)positive products in the surrounding tissue of the cerebral focus was displayed by immunofluorescence staining,and the contents of neuron specific enolate(NSE)and neurite outgrowth inhibitor-A(Nogo-A)in serum(indicating a secondary nerve damage)were assayed by ELISA.Results The mNSS scores were significantly increased on day 1,3,7 and 14 in the model group in comparison with the normal group(P<0.01)and considerably decreased at the 4 time-points after acupuncture intervention relevant to the model group(P<0.05,P<0.01).H.E.staining showed that modeling induced pathological changes such as the excursion of cell nucleus,cellular swelling,vacuole-like change,neuron death,karyopyknosis dissolution,and proliferation of fibrous tissue were relatively milder in the acupuncture group.The average fluorescence intensity values of Iba-1-positive products,serum NSE and Nogo-A contents on day3,7 and 14 were significantly higher in the model group than in the normal group(P<0.05,P<0.01),and notably down-regulated in the acupuncture group than in the model group(P<0.05,P<0.01,except Nogo-A on day 3).Conclusion Acupuncture intervention may accelerate neurological function recovery in TBI rats,which is closely related to its effects in inhibiting the activation of microglia and secondary nerve damage.关键词：Traumatic brain injury;Acupuncture intervention;Microglia;Serum neuron specific enolate;Neurite outgrowth inhibitor-A507|471|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion on cardiac function and the expression of autophagy-related proteins microtubule-associated protein 1 light chain 3(LC3)and selective autophagy receptor signaling adaptor sequestosome 1(SQSTM1/p62)in rats with chronic heart failure(CHF),so as to explore its underlying mechanisms in preventing and treating CHF.Methods Sixty male SD rats were randomly divided into normal,model,moxibustion,autophagy inhibitor 3-methyladenine(3-MA)and autophagy agonist rapamycin(RAPA)groups(n=12 rats/group).The CHF model was established by intraperitoneal injection of adriamycin(ADR,2 mg/kg,once every week for 12 weeks).Mild moxibustion was applied to bilateral"Feishu"(BL13)and"Xinshu"(BL15)for 20 min every time.Rats of the 3-MA group were treated by intraperitoneal injection of 3-MA suspension(15 mg/kg),and those of the RAPA group treated by gavage of RAPA suspension(2 mg/kg).All the treatments were given once a day for 3 weeks.The heart rate(HR),cardiac output(CO),left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP)and maximum rising and lowering rates of left ventricular pressure(±dp/dtmax)were measured for assessing the cardiac performance.Histopathological changes of the left ventricular myocardium were observed by HE staining.The expression levels of LC3-Ⅰ,LC3-Ⅱ and p62 proteins of the left ventricle myocardium tissue were detected by Western blot.Results After modeling,the pathological changes of myocardium(as myocardial cell swelling with vacuoles,myocardial fibre breakage,etc.)were obvious,and the HR,LVEDP,LC3-Ⅱand LC3-Ⅱ/Ⅰ protein expression levels were significantly increased in the model group compared with the normal group(P<0.01),while the CO,LVSP,±dp/dtmax,and the expression of p62 protein were significantly down-regulated(P<0.01).Following the interventions,the myocardial injury was reduced,the HR,LVEDP,LC3-Ⅱ and LC3-Ⅱ/Ⅰ levels in both moxibustion and 3-MA groups were significantly decreased(P<0.05,P<0.01),while the CO,LVSP,±dp/dtmax and p62 expression level were significantly increased relevant to the model group(P<0.05,P<0.01).In addition,the ratio of LC3-Ⅱ/Ⅰ was significantly increased,and the expression level of p62 significantly down-regulated in the RAPA group compared with the model group(P<0.01).Conclusion Moxibustion can improve cardiac function in CHF rats,which may be related to its effects in down-regulating the ratio of LC3-Ⅱ/Ⅰ and up-regulating the expression of p62 protein to inhibit cardiomyocyte autophagy.关键词：Moxibustion;Chronic heart failure;Myocardial cell autophagy;Microtubule-associated protein 1 light chain 3;Sequestosome 1(SQSTM1/p62)690|372|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of "Neiguan"(PC6)-electroacupuncture(EA)or moxibustion(Moxi)pretreatment on myocardial apoptosis and expression of autophagy related proteins light chain(LC)3-Ⅰand LC3-Ⅱin rats with myocardial ischemia/reperfusion injury(MIRI),so as to explore their mechanisms underlying improvement of MIRI.Methods Forty SD rats(half male and half female)were randomly divided into sham operation,model,ischemic preconditioning(IP),EA and Moxi groups(n=8 in each group).EA(10 Hz/50 Hz,1 mA)or Moxi(ignited moxa stick)was respectively applied to bilateral "Neiguan"(PC6)for 20 min,once daily for 7 days.The MIRI model was established by occlusion of the anterior descending branch of the left coronary artery for 40 min,followed by reperfusion for 60 min.The ultrastructural changes and autophagy of myocardial cells were observed by electron microscopy(EM),and the myocardial cellular apoptosis[apoptotic index=(number of apoptotic cells/total number of cardiomyocytes)×100%]was detected by the terminal deoxyribonucleotidyl transferase mediated dUTP nick end labelling(TUNEL)method.The expressions of LC3-Ⅰand LC3-Ⅱproteins(markers for autophagy)in myocardial tissue were detected by Western blot.Results Following MI,EM observation revealed a vague structure of cardiomyocytes and muscular horizontal grain,dissolution of myofibers,mitochondrial swelling,some autophagic vacuoles and autophagic lysosomes at different degrees and surrounded by a double membrane in the model group,these situations were apparently milder in the EA and Moxi groups.The apoptosis index,myocardial LC3-Ⅰ and LC3-Ⅱ protein expression levels,and the ratio of LC3-Ⅱ/Ⅰwere significantly increased in the model group relevant to the sham operation group(P<0.05).After the treatment,the apoptosis index,the expression level of myocardial LC3-Ⅱ protein and the ratio of LC3-Ⅱ/Ⅰ were considerably down-regulated in the IP,EA and Moxi groups in comparison with those in the model group(P<0.05).The effect of EA was obviously superior to those of IP and Moxi in down-regulating the apoptosis index(P<0.05),but obviously inferior to those of IP and Moxi in down-regulating the levels of LC3-Ⅱ and LC3-Ⅱ/Ⅰ(P<0.05).No significant changes were found in the expression of LC3-Ⅰ after IP,EA and Moxi interventions in comparison with the model group(P>0.05),and no significant differences were observed in the apoptosis index and levels of LC3-Ⅱ and LC3-Ⅱ/Ⅰ between the IP and Moxi groups(P>0.05).Conclusion Both EA and moxibustion pretreatments,similar to IP,have a positive role in reducing myocardiocyte apoptosis and regulating autophagy-related protein expression in MIRI rats,which maybe contribute to their protective effects on ischemic myocardium.关键词：Electroacupuncture pretreatment;Moxibustion pretreatment;Myocardial ischemia/reperfusion injury;Ischemic preconditioning;Cardiomyocyte apoptosis;Autophagy687|833|10更新时间：2023-08-11
- 摘要：Objective To explore the effect of electroacupuncture(EA)on amyotrophia and expression of paired box7(Pax7),myogenic differentiation antigen-1(Myod1),myogenin(Myog),myosin heavy chain-Ⅱa(Myh2),myosin heavy chain-Ⅱx(Myh1)and myosin heavy chain-Ⅰ(Myh7)of denervated gastrocnemius in rats with chronic constriction injury(CCI)of the right sciatic nerve,so as to explore its mechanisms underlying postponing development of amyotrophia.Methods Sixty-six SD adult male rats were randomly divided into sham operation(sham)group(n=24),model group(n=24)and EA group(n=18).The denervated muscle(gastrocnemius)atrophy model was established by CCI of the right sciatic nerve.EA(2 Hz,1.0 mA)was applied to the right"Zusanli"(ST36)and"Huantiao"(GB30)for 10 min,once a day,six times a week and for 1,2 and 4 weeks,respectively.After complete dissection of bilateral gastrocnemius muscles,their wet weight levels were measured and the ratio of wet weight(=that of the operation side/that of the non-operation side)was calculated,and the cross-sectional area(CSA)and diameter of the gastronemius were detected after fixation in 4%paraformaldehyde,sectioning,and H.E.staining.The expression levels of Pax7,Myod1,Myog,Myh2,Myh1 and Myh7 mRNAs in the gastrocnemius tissue after 3 weeks of modeling were detected with quantitative real time-PCR(qPCR).Results After 1 week of modeling,the ratios of wet weight of gastrocnemius and the CSA and fiber diameter at the 2 nd,3 rd and 5 th week were significantly smaller in the model group than in the sham group(P<0.05).The expression levels of Myod1 and Myog mRNAs were significantly up-regulated(P<0.01),and those of Myh2,Myh1 and Myh7 considerably down-regulated in the model group compared with the sham group(P<0.05,P<0.01).No significant changes were found in the expression levels of Pax7 mRNA after modeling and EA intervention(P>0.05).Following EA intervention,the CSA and diameterof the gastronemius were obviously increased(P<0.05),and the expression levels of Myod1,Myog and Myh7 further markedly or remarkably up-regulated in comparison with the model group(P<0.05,P<0.01).No significant changes were found in the ratio of wet weight of gastrocnemius at the 3 time-points,and the expression levels of Myh2 mRNA and Myh1 mRNA in the EA group relevant to the model group after 3 weeks of modeling(P>0.05).Conclusion EA treatment may delay gastrocnemius atrophy in CCI rats,which is possibly associated with its effects in up-regulating the expression of Myod1,Myog and Myh7 mRNAs to control the differentiation of the satellite cells and the muscle fiber type transformation.关键词：Denervated skeletal muscle atrophy;Electroacupuncture;Differentiation of muscle satellite cells;Muscle fiber type transformation563|399|7更新时间：2023-08-11
- 摘要：Objective To observe the clinical therapeutic effect of acupuncture of Huatuo Jiaji(EX-B2)in the treatment of gastrointestinal dysfunction in sepsis patients,aiming at providing new clinical approach and evidence for improving septic gastrointestinal activity.Methods A total of 40 patients with septic gastrointestinal dysfunction who met our inclusive criteria were randomly divided into control group and treatment group(n=20 cases in each).Patients in the control group received routine treatment of nasogastric mosapride citrate(5 mg/time,three times a day)and tifidobacterium triple viable capsules(420 mg,twice a day),intravenous infusion of omeprazole(40 mg,twice a day),rational dose of antibiotics,maintenance treatment of functions of the heart,lung,brain,kidney and other important organs and water electrolyte balance,as well as symptomatic treatment.Patients of the treatment group were treated by acupuncture of EX-B2 on the basis of routine treatment mentioned above in the control group.The acupuncture needle was inserted into EX-B2,twirled for a while and retained for 30 min,once a day for10 successive days.The acute physiology and chronic health evaluation(APACHE II)score was given according to Knaus and colleagues' method,the intra-abdominal pressure was detected by using a manometer which was connected to an inserted canal in the urinary bladder,and the intragatric residul volume detected by using an injection syriange pumping via a nasal feeding tube.Results After the treatment,the APACHE II score,intra-abdominal pressure and intragastric residual volume on day 10 were significantly decreased in both groups in comparison with their own pre-treatment(P<0.05),while the borborygmus levels on day 10 were considerably increased in both groups relevant to their own pre-treatment(P<0.05).The APACHE II scores,intraabdominal pressure and intragastric residual volume on day 3,6 and 10 were significantly lower in the treatment group than those in the control group(P<0.05),whereas the borborygmus levels at the 3 rd,6 th and 10 th day in the treatment group were apparently higher than those of the control group(P<0.05).Conclusion Acupuncture stimulation of EX-B2 has a good curative effect in improving gastrointestinal dysfunction in sepsis patients.关键词：Huatuo Jiaji points(EX-B2);Sepsis;Gastrointestinal dysfunction;Intra-abdominal pressure;Gastric residual volume;Borborygmus511|407|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of deep acupuncture of Tiantu(CV22),etc.in combination with rehabilitation training on swallowing function of patients with post-stroke dysphasia.Methods A total of 70 patients suffering from poststroke dysphagia were randomly divided into control group(conventional acupuncture+swallowing rehabilitation training)and treatment group(deep acupuncture of CV22+rehabilitation training,n=35 cases in each group).The swallowing rehabilitation procedure was conducted 30 min every time,twice daily,5 times a week for 3 weeks.In patients of the treatment group,after deep insertion(about 80 mm)along the posterior margin of the manubrium sterna,the filiform needle in CV22 was lifted and thrusted repeatedly 9 times till the patient experienced a feeling of foreign matter blocking,followed by retaining the needle for 30 min.For patients of the control group,Lianquan(CV23)was punctured with 3 acupuncture needles(one vertical needling and bilateral slope needling toward the tongue root),and Fengfu(GV16),bilateral Bailao(EX-NH23),bilateral Renying(ST9)and bilateral Jialianquan(Extra)were punctured vertically with filiform needles.The treatment was conducted once daily,5 times a week for 3 weeks.The swallowing function was evaluated by using Kubota water swallowing test(KWST)scale(0-5 points),Fujishima Ichiro rating scale(FIRS,0-10 points),and video fluoroscopic swallowing study(VFSS,0-10 points),separately.The therapeutic effect was also assessed according to the clinical scale scores and VFSS results.Results Following the treatment,of the two 35 cases in the control and treatment groups,4(11.43%)and 8(22.86%)experienced marked improvement in their symptoms,22(62.86%)and 23(65.71%)were effective,and 9(25.71%)and 4(11.43%)were failed,with the effective rates being 74.29%and 88.57%,respectively.After the treatment,the KWST score of the two groups were significantly decreased in comparison with their own pre-treatment(P<0.05),while the FIRS and VFSS scores of the two groups were considerably increased in comparison with their own pre-treatment(P<0.05).The therapeutic effects of the treatment group were obviously superior to those of the control group in the effective rate,lowering KWST score and in raising FIRS and VFSS scores(P<0.05).Conclusion Acupuncture plus swallowing rehabilitation training is effective in improving the swallowing function of stroke patients with dysphagia,and the deep acupuncture stimulation of CV22 is apparently better than conventional acupuncture in the therapeutic effect.关键词：Acupuncture therapy;Post-stroke dysphagia;Deep acupuncture at Tiantu(CV22);Swallowing rehabilitation training525|561|6更新时间：2023-08-11
- 摘要：Objective To observe the short-term and long-term therapeutic effects of internal heat-type acupuncture needle(IHTAN)therapy combined with acupoint injection of O_3 for post-stroke shoulder pain in apopletic patients.Methods Patients with post-stroke shoulder pain were divided into warm needle moxibustion+ acupoint injection group(29 cases),acupoint injection group(30 cases)and IHTAN+acupoint injection group(29 cases)according to the random number table.For acupoint injection,O_3 solution(3 mL,30μg/mL)was injected into Jianyu(LI15),Jianliao(SJ14),Jianzhen(LI9),Binao(LI14)and Ashi points(4—6 acupoints every time)on the affected side,once every other day for 4 weeks.For warm needle moxibustion,the above mentioned acupoints were stimulated with filiform needles attached with an ignited moxa-stick,once every other day for 4 weeks.For IHTAN,the internal warmth controllable acupuncture needles were inserted into the above mentioned acupoints on the affected side,and then connected to an internal heat acupuncture apparatus for stimulating the acupoints at 42 ℃,20 min every time,once a week,for 4 weeks.The severity of shoulder pain was assessed by using visual analogue scale(VAS,0-10 points),the upper limb joint motion function evaluated using Fugl-Meyer assessment scale(FMA,0-66 points),and the ability of daily living activities(ADL)evaluated using Barthel index(0-100 points)at the ends of the 2 nd and 4 th week as well as the 6 th month after the treatment.If the shoulder pain disappeared or basically disappeared,it was considered to be healed.If the pain was relieved,it was considered to be effective.Otherwise,it was considered to be ineffective.Results At the ends of the 2nd and 4th week as well as the 6th month after the treatment,the VAS scores of the IHTAN+acupoint injection group were significantly lower than those of the warm needle moxibustion+acupoint injection and acupoint injection groups(P<0.05),and the FMA and ADL scores were significantly higher in the IHTAN+acupoint injection group than in the other two groups(P<0.05).No significant differences were found between the warm needle moxibustion+acupoint injection and the acupoint injection groups in the scores of VAS,FMA and ADL(P>0.05).Of the 29,30 and 29 cases in the warm needle moxibustion+acupoint injection,acupoint injection and IHTNA+acupoint injection groups,1,0 and 7 cases at the 2nd week,3,2 and 12 cases at the 4th week,4,2 and 15 cases at the 6 th month were cured;15,14 and 16 cases at the 2nd week,17,17 and 13 cases at the 4th week,and 18,18 and 12 cases at the 6th month experienced marked improvement;13,16 and 6 cases at the 2nd week,9,11 and 4 cases at the 4th week,7,10 and 2 cases at the 6th month had no apparent changes in their shoulder pain severity,with the total effective rates at the 4th week and 6th month being 68.97% and 75.86%,63.33% and 66.60%,and 86.21% and 93.10%,respectively.Conclusion The internal heat-type acupuncture needle therapy combined with O_3 acupoint injection has obvious short-term and long-term therapeutic effects for post-stroke shoulder pain and is obviously superior to warm needle moxibustion+ acupoint injection and simple acupoint injection in relieving shoulder pain and improving upper limb motion function.关键词：Post-stroke shoulder pain;Internal heat-type acupuncture needle therapy;Acupoint injection;Warm needle moxibustion;Upper limb motion function628|430|5更新时间：2023-08-11
- 摘要：Objective To observe the therapeutic effect of electroacupuncture(EA)and acupoint catgut embedding in the treatment of simple obesity.Methods Simple obesity patients were randomized into EA group(7 men and 36 women,21-42 years in age)and catgut embedding group(4 men and 37 women,22-41 years in age).EA(4 Hz/20 Hz,a tolerable strength)was applied to main acupoints Zhongwan(CV12),bilateral Tianshu(ST25),Daheng(SP15),Daimai(GB26),Shuidao(ST28),Zhigou(TE6),Yinlingquan(SP9),Zusanli(ST36),Fenglong(ST40),and Sanyinjiao(SP6),and some auxiliary acupoints for 30 min,once every other day for 30 times.Subcutaneous catgut-embedment was performed in the same acupoints.Nine to 11 acupoints were used every time,once every 10 days for 6 times.Before and after the treatment,fasting serum leptin and insulin(INS)contents were detected by radioimmunoassay,and the correlation between the leptin,INS and the body mass index(BMI)was analyzed,respectively.Results Following the treatment,the serum leptin and INS concentrations and BMI in both groups were significantly decreased in comparison with those of their own pre-treatment(P<0.01).No significant differences were found between the two groups in the levels of serum leptin and INS after the treatment(P>0.05).There were positive correlations between the decreased BMI and serum leptin/INS contents in both EA and catgut embedding groups(P<0.01).Conclusion Both catgut embedding and EA interventions have a positive effect in reducing body weight of simple obesity patients,which may be related to its effects in down-regulating serum leptin and INS levels and in correcting leptin resistance and insulin resistance.关键词：Catgut embedding;Electroacupuncture;Simple obesity;Leptin;Insulin577|996|6更新时间：2023-08-11
- 摘要：Objective To try to re-map an acupoint atlas in vector mode in the experimental rat by using a new-generation drawing tool.Methods A total of 5 SD rats were used in the present study.In reference to the current acupoint locations and anatomical structure of rats as well as those of the human body,an acupoint atlas having a stereoscopic mode was re-mapped by using Adobe photoshop/illustrator CS6 imaging processing and drawing system.Results According to the skeletal structure and body surface landmarks,an acupoint atlas consisting of Shuigou(GV26),Baihui(GV20),Tianmen(i.e.Cuanzhu,BL2),Erjian(MA-H6),Dazhui(GV14),Feishu(BL13),Xinshu(BL15),Geshu(BL17),Jizhong(GV6),Pishu(BL20),Shenshu(BL23),Houhui(SI3),Huantiao(GB30),Houhai,Yanglingquan(GB34),Housanli(ST36),Zhaohai(KI6),Sanyinjiao(SP6),Genduan point,Shenmai(BL62),Taichong(LR3),Zhijian(foot),Yongquan(KI1),Guanyuan(CV4),Xiqian,Weijian,Shenque(CV8),Zhongwan(CV12),Qiansanli(LI10),Waiguan(SJ5),Neiguan(PC6),Quchi(CV6),Zhoujie(EX-UE1),Hegu(LI4),Zhijian(hand),Houxi(SI3),Shenmen(HT7),Taiyuan(LU9),Shaohai(HT3),Chize(LU5),Danzhong(CV17),and Chengjiang(CV24)which have been positioned in plane graph mode by Professor HUA Xing-bang and colleagues in 1991.The newly re-mapped acupoint atlas displayed more clearly in the anatomic locations relevant to the traditional one.Conclusion A new acupoint atlas with 3 dimension image mode is accomplished in the rat,being more convenient and applicable for researchers to edit the relevant graph materials in paper writing,and playing apossibly useful guidance for the standardization of acupoint selection in experimental acupuncture researches.关键词：Acupoint atlas;Standardization;Anatomy;Vectograph;Rat543|13|18更新时间：2023-08-11
- 摘要：Objective To determine the attributes of responses of the higher nerve center to acupuncture stimulation of Zusanli(ST36)on the basis of spontaneous brain activity,so as to explore the synchronization level of different brain rejoins after acupuncture.Methods All studies using fMRI to investigate the effect of acupuncture stimulation of ST36 and/or other acupoints on the human brain(at least 10 healthy subjects or patients in one group)published in journals from January of 1995 to January of2018 were searched from databases of CNKI and PubMed by using keywords of acupuncture,electroacupuncture,and resting-state functional magnetic resonance images(rs-fMRI)or regional homogeneity(ReHo).Brain functional image data of acupuncture stimulation of ST36 and/or other acupoints were collected and analyzed with anisotropic effect size-signed differential mapping(AESSDM)software(Meta-analysis),and those of acupuncture of simple ST36 analyzed as a subgroup.Results A total of 229 papers in Chinese and 109 in English were collected.According to our inclusive and exclusive standards,11 papers containing 235 subjects were brought into analysis at last.Meta-analysis of brain image data of acupuncture at ST36 and/or other acupoints(comparison between pre-and post-acupuncture)revealed that the same brain regions(generality)which showed a significant increase in ReHo,are the right and left anterior cingulated gyrus,right caudate,left superior frontal gyrus,right middle frontal gyrus,and the right paracentral lobe,and those which showed a marked decrease of ReHo are the left mid-inferior occipital gyrus,left and right precentral and postcentral gyrus.The brain regions responding to acupuncture at ST36 only(specificity)are the right inferior parietal lobe,left middle inferior gyrus,right posterior lobe of cerebellum,and the left angular gyrus which displayed an increase of ReHo,and the right middle superior frontal gyrus which showed a decrease in ReHo.Conclusion After acupuncturing at ST36,the relative generality and specificity of the central response in healthy subjects reflected as the location of the affected brain regions and the difference in the synchronization level of the corresponding spontaneous brain activities.关键词：Acupuncture;fMRI;Zusanli(ST36);Spontaneous brain activity;Regional homogeneity;Generality;Specificity573|436|2更新时间：2023-08-11
- 摘要：Large number of microflora is parasitized in the human intestinal tract,which maintains the stability of the microecological environment in the host's intestinal tract and the healthy state of the body.Once the steady state is out of balance,the intestinal microflora is dysfunctional and a variety of diseases will be induced.Acupuncture and moxibustion have a positive role in improving different types of clinical problems by regulating qi,harmonizing yin and yang,and strengthening the body's resistance.At present,researches on the effect of acupuncture and moxibustion interventions on intestinal microflora mainly focuses on the following aspects 1)adjusting the count and proportion of the intestinal microflora to recover its stability,2)improving gastrointestinal motility disorder by promoting the interaction between intestinal microflora and brain-gut axis(brain-gut peptides),and ameliorating the intestinal barrier function by reducing levels of proinflammatory cytokines to suppress inflammatory reactions.In the future,the research should pay more attention to the holistic regulatory function of acupuncture and moxibustion,the acupoint specificity,acupoint combination and different intervention measures,as well as the optimization of clinical regimen,so as to better intestinal microflora regulation.关键词：Acupuncture-moxibustion;Intestinal microflora;Current research situation;Research ideas606|1511|9更新时间：2023-08-11
- 摘要：Endoplasmic reticulum stress(ERS)is one of the major defense networks in mammals,but continuous ERS will lead to cell apoptosis,inducing initiation and progression of many human diseases.Biochemical,physiological and pathological stimuli induced ERS may help the cells adapt to and survive stress conditions by activating unfolded protein response(UPR),ERoverload response and sterol modulation cascade reaction.Abundant outcomes of clinical and experimental studies in recent5 years showed that acupuncture and moxibustion therapies are effective in improving some symptoms of nervous system diseases as stroke,Parkinson's disease,convulsion,cardiovascular diseases,diabetes mellitus,non-alcoholic fatty liver disease,spinal cord injury,muscular injury,etc.,which is closely associated with their functions in relieving excessive ERS and cell apoptosis via modulating UPR pathway.Some important molecules or signaling pathways such as glucose-regulated protein 78(GRP78),Caspase-12,ER transmembrane kinase inositol-requiring and ER-to-nucleus signaling protein(IRE1 P),phosphorylated extracellular signal-regulated kinase(p-ERK)/eukaryotic translation initiation factor 2α(eIF2α),activating transcription factor 4(ATF4),CCAAT/enhancer-binding protein homologous protein(CHOP),etc.have been demonstrated to be involved in the effects of acupuncture-moxibustion interventions.But,the mechanism of acupuncture-moxibustion underlying improvement of ERS remains far more unclear.Future studies targeting the combination of molecules and holistic function,verification of changes of the involved protein molecules and multiple signaling pathways,balancing regulation between the cell survival and apoptosis,etc.by adopting syndrome-differentiation-based acupoints are definitely needed.关键词：Endoplasmic reticulum stress;Acupuncture;Nervous system disease;Diabetes;Cardiovascular disease;Non-alcoholic fatty liver disease602|788|7更新时间：2023-08-11
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