最新刊期
卷 44 ， 期 11 ， 2019
- 摘要：Objective To observe the effect of electroacupuncture(EA)on the expression of apolipoprotein E(ApoE)and related proteins of inflammation and anti-oxidative stress in spinal cord in mice with spinal cord injury(SCI),so as to explore its mechanisms underlying function repair.MethodsThirty-six female C57BL/6 mice were equally randomized into 3 groups:sham operation,model and EA. The SCI model was established by clamping the spinal cord for 25s with a serrefine after laminectomy of the 1 stlumbar vertebra(L1). EA(1. 5 Hz/7. 5 Hz,1. 0 mA)was applied to bilateral"Zusanli"(ST36)and"Sanyinjiao"(SP6)for 10 min,once a day for 7 days. The hindlimb locomotor function was assessed according to the state of the range of motion,coordination,claw gesture of the hind leg ankle-joint,trunk stability and the tail posture by using Basso Mouse Scale(BMS). The histopathological changes of the injured area of the spinal cord were determined by H. E. staining. The expression levels of Apo E,phosphorylated nuclear transcription factor-κB(p-NF-κB),interleukin 1 beta(IL-1β),phosphorylated extracellular regulatory protein kinase(p-ERK1/2),extracellular regulatory protein kinase(ERK1/2),nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxidase-1(HO-1)in the spinal cord were detected by Western blot,and the glial fibrillary acidic protein(GFAP)-positive astrocytes were displayed by immunofluorescence staining.ResultsAfter modeling,the BMS scores were significantly decreased in the model group compared with the sham operation group(P<0. 05). Following EA,the BMS scores were markedly increased in the EA group relevant to the model group(P<0. 05),suggesting an improvement of the hindlimb locomotor function. H. E. stain showed structural disorder with lots of cavities,severe inflammatory infiltration with large quantity of inflammatory cells,and apparent reduction of normal neurons in the injured spinal cord tissue of model group,which was milder in the EA group. The expression levels of Apo E,p-NF-κB,IL-1β,p-ERK1/2(not ERK1/2),Nrf2 and HO-1 were significantly increased in the model group than those in the sham operation group(P<0. 05). Compared with the model group,the expression levels of Apo E,p-ERK1/2,Nrf2 and HO-1 were further notably up-regulated(P<0. 05),and those of p-NF-κB and IL-1β proteins obviously down-regulated in the EA group(P<0. 05). Immunoflorescence staining showed that the number of GFAP-positive cells was apparently increased in the model group compared with the sham operation group and observably decreased in the EA group relevant to the model group(P<0. 05).Conclusion EA can significantly improve locomotor function in SCI mice,which is associated with its effects in reducing inflammation,oxidative stress reactions and reactive astrocyte proliferation via up-regulating expression of Apo E,p-ERK1/2,and Nrf2/HO-1(antioxidant pathway)and inhibiting IL-1β and NF-κB expression.关键词：Spinal cord injury;Electroacupuncture;Hindlimb locomotion function;Apolipoprotein E;Nrf2/HO-1 antioxidant pathway;Nuclear transcription factor-κB;Astrocyte536|536|8更新时间：2023-08-10
- 摘要：Objective To observe the influence of different courses of electroacupuncture(EA)intervention on recognition memory and the proliferation and differentiation of hippocampal neural stem cells in mice with radiation-induced brain injury,so as to explore its mechanisms underlying improving radiation-induced brain injury.MethodsSeventy 30-day old C57BL/6 J mice were randomly divided into control,model and EA groups,and the latter two groups were further divided into 1 week(W),2 W and 3 W subgroups(n=10 in the control group and each subgroup). The radiation-induced brain injury model was established by radiating the mouse'left head at a dose of 8 Gy for 10 min by using a radiation linear accelerator. EA(1. 5 V,2 Hz/10 Hz)was applied to"Baihui"(GV20),"Fengfu"(GV14)and bilateral"Shenshu"(BL23)for 30 min,once daily for 1,2 and 3 weeks,respectively. The learning-cognition memory ability was detected by using novel object recognition test in an open test box to record the time for exploring a novel object(TN)and a familiar object and to calculate the recognition index(RI). The neural stem cells' proliferation and differentiation in the hippocampus tissues were evaluated by counting the number of bromodeoxyuridine(BrdU)-labeled cells,neuronal nuclei(NeuN)/BrdU-positive cells and BrdU/glia fibrillary acidic protein(GFAP)-positive cells under microscope after immunofluorescence stain.ResultsAfter modeling,the TN at 90 min and 24 h and RI of the model subgroup 3 W at 90 min and RI of the model subgroup 1,2 and 3 W at 24 h were significantly decreased in comparison with those of the control group(P<0. 01,P<0. 05). Moreover,the number of BrdU-positive cells in the model subgroup 1 W and 2 W,the BrdU/NeuN double-labeled cells in the 3 model subgroups and BrdU/GFAP double-labeled cells in the model subgroup 1 W and 3 W were significantly decreased(P<0. 01,P<0. 05). Following EA interventions,the TN in the 3 EA subgroups at both 90 min and 24 h,and RI of EA subgroup 3 W at 90 min and EA subgroup 2 W and 3 W at 24 h were considerably increased compared with those of the corresponding 3 model subgroups(P<0. 05,P<0. 01). The numbers of BrdU-positive cells as well as BrdU/NeuN and BrdU/GFAP double-labeled cells were significantly increased in the 3 EA subgroups(P<0. 05,P<0. 01,P<0. 001).ConclusionEA of GV20,GV14 and BL23 can improve the recognition memory ability of mice with radiation-induced brain injury,which may be related to its effect in promoting the proliferation and differentiation of stem cells in the hippocampus.关键词：Radiation-induced brain injury;Electroacupuncture;Recognition memory;Neural stem cell;Proliferation and differentiation;Hippocampus523|346|6更新时间：2023-08-10
- 摘要：Objective To observe the influence of scalp-acupuncture on the expression of pentraxin 3(PTX3),Interleukin(IL)-1β,zonula occludens-1(ZO-1)mRNA and Occludin mRNA in striatum in acute ischemic cerebrovascular disease(AICD)rats,so as to investigate its mechanisms underlying improvement of AICD.MethodsForty-eight male SD rats were randomly allocated to control,model,IL-1 Ra and IL-1 Ra+scalp-acupuncture groups(n=12 rats in each group). The AICD model was established by occlusion of the middle cerebral artery(MCAO). Rats of the IL-1 Ra group and IL-1 Ra+scalp-acupuncture group received intraperitoneal injection of IL-1 Ra(0. 05 mg·kg(-1)·d(-1)·d(-1)),once daily for 6 days. Scalp acupuncture stimulation was applied to bilateral"Dingnieqianxiexian"(MS6)once daily for 6 days for rats in IL-1 Ra+scalp-acupuncture group. Before and after intervention,the neurologic deficit score(NDS)was evaluated according to Longa's method. The expression of striatum PTX3 and IL-1β was detected by immunohistochemistry,and ZO-1 mRNA and Occludin mRNA in the striatum tissue were detected by fluorescence quantitative real-time PCR.The Evans Blue(EB)tracer method was used to monitor the degree of blood-brain barrier(BBB)damage.ResultsFollowing modeling,the NDS,EB content and the expression of PTX3 and IL-1β in the striatum tissue were significantly increased,and the ZO-1 mRNA and Occludin mRNA expression was considerably decreased in the model group compared with the control group(P < 0. 05). After the interventions and compared with the model group,the NDS,EB content in both IL-1 Ra and IL-1 Ra+scalp acupuncture groups,and PTX3 in the IL-1 Ra group were significantly downregulated(P<0. 05),while the striatum ZO-1 mRNA and Occludin mRNA expression in both IL-1 Ra and IL-1 Ra+scalp acupuncture groups,and PTX3 in the IL-1 Ra+scalp acupuncture group were obviously up-regulated(P<0. 05),and the expression of IL-1β was obviously down-regulated in the IL-1 Ra+scalp acupuncture group(P<0. 05)rather than in the IL-1 Ra group(P>0. 05). The effects of scalp acupuncture combined with IL-1 Ra were obviously superior to that of IL-1 Ra in down-regulating NDS,EB content and IL-1β expression level,and in up-regulating PTX3,ZO-1 mRNA and Occludin mRNA expression levels(P<0. 05).ConclusionScalp acupuncture can improve neurological function and reduce the degree of BBB injury in AICD rats,which may be associated with its function in up-regulating the expression of PTX3 and in promoting the expression of ZO-1 mRNA and Occludin mRNA.关键词：Scalp acupuncture;Acute ischemic stroke;Pentraxin 3;Interleukin-1β;Zonula occludens-1;Occludin;Interleukin-1Ra564|340|4更新时间：2023-08-10
- 摘要：Objective To explore the effect of electroacupuncture(EA)serum on expression of myogenic differentiation antigen(Myod)and autophagy-related protein Beclin 1 in cultured muscle satellite cells of rats under starvation conditions.MethodsThe primary multifidus muscle satellite cells of one male SD rat were isolated and cultured to obtain the 3(rd) generation of cells. The EA serum was got from the rat received EA stimulation of bilateral"Weizhong"(BL40,2 Hz/10 Hz,1 mA,duration of 20 min,once daily for 7 days). The cell suspension(2×10(rd) generation of cells. The EA serum was got from the rat received EA stimulation of bilateral"Weizhong"(BL40,2 Hz/10 Hz,1 mA,duration of 20 min,once daily for 7 days). The cell suspension(2×104/well)of the 34/well)of the 3(rd)generation of cultured cells was transferred to each well of a 96-well plate in medium containing 10% fetal bovine serum(FBS).Twelve duplicate wells were set up for the blank control serum(without FBS),10% FBS,10% EA serum,20% EA serum and 30% EA serum groups and incubated for 12 h and 24 h,respectively. Each well was supplemented with 10 μL CCK-8 reagent to be incubated for 1 h again for observing the state of cell proliferation. After culturing the primary muscle satellite cells in serum-free medium for 12 h,the cells were randomly divided into serum-free group,10% fetal bovine serum group and optimal concentration electroacupuncture serum group,and serum of corresponding concentration was added respectively. The expression levels of Beclin 1 and cell-proliferation-related protein Myod were detected by Western blot.ResultsCCK-8 assay displayed that the proliferation levels were significantly higher at 12 h and 24 h after serum intervention in the 10% FBS,10% EA serum,20% EA serum and 30% EA serum groups than that in the blank control serum group(P<0. 01),and at 24 h in the 3 EA serum groups than in the 10% FBS group(P<0. 01),but without significant difference among the three EA serum groups(P>0. 05). As a result,10% EA serum was selected as the optimal concentration for Western blot tests. No significant difference was found in the expression levels of Myod and Beclin 1 proteins among the serum-free,10% FBS and 10% EA serum groups before intervention(P>0. 05),and there was a marked up-regulation of Myod expression and an obvious down-regulation of Beclin 1 expression at 12 h in both the 10%EA serum and 10% FBS groups in comparison with their own pre-intervention(P<0. 05). There were a marked up-regulation of Myod expression at both 12 h and 24 h and Beclin 1 expression at 24 h in both the 10% EA serum group and10% FBS group than that in the serum-free group(P<0. 05),and an obvious down-regulation of Beclin 1 expression at12 h in both 10% FBS and 10% EA serum groups than that in the serum-free group(P<0. 05,P<0. 01). After 24 h's serum intervention,there was an increase of Myod expression and a reduction of Beclin 1 expression in both 10% FBS and 10% EA serum groups compared with those after the 12 h intervention(P<0. 05). No significant differences were found between the 10% FBS and 10% EA serum groups in the expression levels of Myod and Beclin 1 proteins(P>0. 05).ConclusionEA serum can promote proliferation of cultured muscle satellite cells under starvation conditions,which is related to its functions in regulating expression of Beclin 1 and cell-proliferation-related protein Myod.关键词：Electroacupuncture serum;Cultured muscle satellite cells;Starvation;Autophagy;Myogenic differentiation antigen;Beclin 1528|264|2更新时间：2023-08-10
- 摘要：Objective To observe the effect of electroacupuncture(EA)on behavioral changes,and expression of tyrosine hydroxylase(TH),α-synuclein(α-syn),transcription activating factor 6(ATF6)and transcription factor X box binding protein 1(XBP-1)in the substantia nigra of Parkinson's disease(PD)rats,so as to explore its mechanisms underlying improvement of motor function.MethodsThirty-six male SD rats were randomly divided into control,model and EA groups(n==12 rats in each group). The PD model was established by subcutaneous injection of rotenone(2 mg/kg)at the neck and back,once a day for 28 days. EA(2 Hz,1 mA)was applied to"Fengfu"(GV16)and bilateral"Taichong"(LR3)for 20 min,once a day for 14 successive days. The voluntary motor behavioral changes(total distance,average speed,total movement time,total rest time in 8 min)were detected by open field tests. The immunoactivity of TH and α-syn in the substantia nigra was detected by immunohistochemistry,and the expression of ATF6 mRNA and XBP-1 mRNA detected by fluorescence real-time quantitative PCR.ResultsFollowing modeling and compared with the control group,the total distance,average speed and total movement time of voluntary movement were significantly decreased(P<0. 01),and the total rest time was significantly increased(P<0. 01). Moreover,the expression of TH was significantly decreased(P<0. 01),and that of α-syn protein,ATF6 mRNA and XBP-1 mRNA significantly increased in the model group in comparison with the control group(P<0. 01). After the intervention,the total distance,average speed,and total movement time of voluntary movement in the EA group were considerably higher than those in the model group(P<0. 01),and the total rest time was obviously decreased in the EA group(P<0. 01). The expression level of TH was significantly increased(P<0. 01),and those of α-syn,ATF6 mRNA and XBP-1 mRNA were notably decreased in the EA group compared with the model group(P<0. 01).ConclusionEA intervention can improve the locomotor function in PD model rats,which is associated with its functions in up-regulating the expression of TH protein and down-regulating the expression of α-syn protein,and ATF6 mRNA and XBP-1 mRNA in the substantia nigra of mesencephalon.关键词：Electroacupuncture;Parkinson's disease;Endoplasmic reticulum stress;Transcription activating factor 6;Transcription factor X box binding protein 1531|395|7更新时间：2023-08-10
- 摘要：Objective To explore whether manual acupuncture stimulation of"Shenshu "(BL23),"Guanyuan"(CV4),"Zhongwan"(CV12) can improve the ovarian function by resisting oxidant stress and reducing apoptosis of granulosa cells in rats with ovarian hypofunction.MethodsForty female SD rats with normal estrous cycles were randomly divided into blank control,model,hormone therapy and acupuncture groups(n=10 rats in each group). The ovarian hypofunction model was established by gavage of Tripterygium Glycosides suspension(50 mg·kg(-1)·d(-1)·d(-1))for 14 successive days. Rats of the hormone therapy group were treated by gavage of estrogen and progesterone,and those of the acupuncture group treated by manual acupuncture stimulation of bilateral BL23 or CV4 and CV12 alternatively by using uniform reinforcing-reducing method for 10 s every 5 min(3 times in 10 min). The treatment was performed once daily for 14 days. The blank group was given equal volume of normal saline daily. On the 9 thday,the estrous cycle of each rat was observed by means of vaginal smear test. The ovarian index,serum estradiol(E2),follicle stimulating hormone(FSH),luteinizing hormone(LH),malondialdehyde(MDA)and superoxide dismutase(SOD)contents were detected by ELISA. Histopathological changes of the ovary tissue were observed by H. E. staining. The expressions of apoptosisrelated proteins Bax and Bcl-2 and their mRNAs of the ovaries were determined by immunohistochemical staining and quantitative real time-PCR separately.ResultsThe menstrual disorder rate was 100% in the model group,and was significantly higher than those in the hormone therapy(30%) and acupuncture(40%) groups(P<0. 01). Following modeling,the ovarian wet weight and index,E2 and SOD contents,Bcl-2 protein and mRNA expressions were significantly decreased(P<0. 01,P<0. 05),and serum FSH,LH and MDA levels,Bax protein and mRNA expressions were significantly increased(P<0. 05,P<0. 01)in the model group in comparison with the blank control group. H. E. staining showed a large amount of connective tissue in the ovary,with fewer mature follicles,increase of atresia follicles,significant reduction of luteal tissue,and appearance of scarring tissue in the model group. Compared with the model group,there were more mature follicles,fewer atresia follicles,lower abnormal granulosa cell morphology and lower estrous cycle disorder ratio in both hormone therapy and acupuncture groups. After the interventions,the decreased ovarian wet weight and index,serum E2 and SOD contents,expressions of Bcl-2 protein and mRNA were significantly increased(P<0. 05,P<0. 01),and the increased levels of FSH and LH,MDA,expressions of Bax protein and mRNA were significantly decreased in both hormone therapy and acupuncture groups(P<0. 05,P<0. 01). The effect of acupuncture was significantly superior to that of hormone in up-regulating SOD,Bax and Bcl-2 protein expressions(P<0. 01).Conclusion Acupuncture can improve the menstrual disorder in rats with ovarian hypofunction,which is closely related to its effects in improving antioxidant stress ability and regulating the expressions of apoptosis-related protein and mRNA of ovarian granulosa cells.关键词：Acupuncture;Tripterygium Glycosides;Ovarian hypofunction;Oxidative stress;Apoptosis533|713|14更新时间：2023-08-10
- 摘要：Objective To observe the effect of moxibustion pretreatment at different time on serum hormone levels in diminished ovarian reserve(DOR)rats,so as to explore its protective mechanisms.MethodsForty female SD rats were randomly divided into control,model,moxibustion-1(moxibustion was given 4 weeks before modeling),moxibustion-2(moxibustion was given 2 weeks before modeling and 2 weeks from the 1 st day on after modeling)and moxibustion-3(moxibustion was given 4 weeks from the 1 st day on after modeling)groups(n=8 rats in each group). The DOR model was established by gavage of Tripterygium Glycosides(75 mg/kg)once daily for 14 days. Grain-moxibustion was applied to"Shenshu"(BL23)and"Guanyuan"(CV4)for 7 cones,5 times a week for 4 weeks. The body weight and the ovary weight were recorded for calculating the ovarian index. The levels of serum anti-mullerian hormone(AMH),follicle stimulating hormone(FSH),estradiol(E2),androgen(T)and dehydroepiandrosterone(DHEA)were detected by ELISA.ResultsAfter modeling,ovarian index and serum AMH levels were obviously decreased(P<0. 05),and the levels of serum FSH,E2,T and DHEA were significantly increased in contrast with the control group(P < 0. 05).Following intervention and compared with the model group,the serum FSH and DHEA levels of each moxibustion group were significantly reduced(P<0. 05),the AMH levels significantly increased and E2 and T contents significantly decreased in the moxibustion-2 and moxibustion-3 groups(P<0. 05). The serum FSH,E2 and T contents in moxibustion-2 group were obviously lower than those of the moxibustion-1 and moxibustion-3 groups(P<0. 05).ConclusionMoxibustion pre-treatment can improve ovarian reserve function in DOR rats,while the effect is different with different intervention time,and the best intervention time is pre-occurrence and early stage of DOR.关键词：Moxibustion pretreatment;Diminished ovarian reserve;Time-effect relationship505|531|5更新时间：2023-08-10
- 摘要：Objective To observe the effect of electroacupunctur(EA)and moxibustion of left or right"Tianshu"(ST25)on intragastric pressure(IGP)in normal rats.MethodsTwenty male SD rats were equally and randomly divided into EA group and moxibustion group. EA(2 Hz/15 Hz,2 mA)or moxibustion(46—48 ℃)was applied to unilateral ST25 for 2 min. The IGP was detected by using a pressure-transducer and an amplifier before and after EA or moxibustion.ResultsCompared with pre-intervention,the IGP was significantly decreased after EA or moxibustion stimulation of both left and right ST25(P<0. 05). The IGP levels during 0—30 s and 30—60 s of EA stimulation of both left and right ST25 were significantly lower than those of moxibustion(P<0. 05). The IGP-lowering effect of EA on the left ST25 was evidently stronger than that of the right ST25(P<0. 05),but without significant difference between the bilateral sides in moxibustion-induced IGP reduction(P>0. 05).ConclusionBoth EA and moxibustion of ST25 can lower IGP,and the effect of EA is significantly stronger than that of moxibustion in normal rats. In addition,the IGP-lowering effect of EA of the left ST25 is markedly stronger than that of the right ST25,suggesting a lateral advantage effect.关键词：Electroacupuncture;Moxibustion;Intragastric pressure;Tianshu (ST25)495|373|5更新时间：2023-08-10
- 摘要：Objective To explore the distribution of pressure-and heat-sensitive acupoints of the Large Intestine of Hand-Yangming,Stomach Meridian of Foot-Yangming and the Lung Meridian of Hand-Taiyin below the elbow and knee joints in patients with allergic rhinitis,so as to explore the positive reaction points at the body surface of allergic rhinitis.MethodsThirty variant rhinitis volunteer subjects and 30 healthy subjects were recruited in the present study.The subcutaneous induration,reaction points of emptiness-like feeling or pressing pain below the elbow-and knee-joints along the Large Intestine Meridian,Stomach Meridian and the Lung Meridian were inspected first,then re-examined by using heat-sensitized moxibustion method and a hand-held pain threshold tester(mechanical pressure),respectively. The frequency and coincidence rate of pressing-pain sensitive and heat-sensitive points were analyzed.ResultsIn the 30 patients with allergic rhinitis,10 positive reaction points as Kongzui(LU6),Chize(LU5),Hegu(LI4),Quchi(LI11),Shousanl(iLI10),Zusanli(ST36),Shangjuxu(ST37),Fenglong(ST40),Xiajuxu(ST39),and non-acupoint of the Lung Meridian were found. Among them,the duration of heat-sensitive reaction in the LU6,LI10,ST37,ST40,and ST39 regions was obviously shorter in patients than in healthy subjects(P<0. 01,P<0. 005). The heat-sensitive forms of the positive reaction points were mainly heat transmission,heat-penetration and local reaction. The mechanical pain thresholds of the positive reaction points(LI4,LU6,LI10,LI11 and non-acupoint of the Lung Meridian)at the upper limbs and those(ST36,ST37 and ST40)at the lower limbs were evidently lower in patients than in healthy volunteer subjects(P<0. 05). The pressure-and heat-sensitive acupoints were basically coincide in the patients,but being higher in patients than in healthy subjects in the frequency of occurrence. The mechanical pain threshold of the pressure-sensitive acupoints in patients was remarkably lower on the left side than on the right side of the upper limb,and obviously higher on the left side than on the right side of the lower limb(P<0. 05). No significant differences were found between the right and left sides in the pain threshold in healthy subjects(P>0. 05).ConclusionThere is a high overlap in the distribution of pressure-and heat-sensitive acupoints of the Lung,Large Intestine and Stomach meridians below the elbow and knee joints in patients with allergic rhinitis,and there is an imbalance between the left and right limbs in the mechanical pain threshold of the pressure-and heat-sensitive acupoints,but needing being further confirmed.关键词：Allergic rhinitis;Pressure-sensitive acupoints;Heat-sensitive acupoints;Distribution characteristics;Mechanical pain threshold558|549|2更新时间：2023-08-10
- 摘要：Objective To observe the efficacy of"Buqi Yixue "needling on clinical symptoms,neurological function and nerve conduction velocity in patients with diabetic peripheral neuropathy.MethodsEighty-six patients with diabetic peripheral neuropathy numbness and pain were equally randomized into control group and treatment group. The patients of the control group received basic treatment and oral administration of Cilostazol(50 mg/time,2 times/d)and Epalrestat(50 mg/time,3 times/d). The patients of the treatment group received acupuncture stimulation of Danzhong(CV17),Qihai(CV6),Pishu(BL20),Quchi(LI11),etc.,for 30 min,once every day,on the basic treatment. The treatment was conducted for 8 successive weeks. The scores of Traditional Chinese Medicine(TCM)symptoms,Toronto clinical scoring system(TCSS)and nerve conduction velocity(NCV)were detected before and after the treatment.ResultsAfter the treatment,the scores of TCM symptoms and TCSS were considerably decreased(P<0. 05),and the NCV was significantly increased(P<0. 05)in both groups compared with those of their own pre-treatment. The therapeutic effect of the treatment group was significantly superior to that of the control group in lowering the scores of TCM symptoms and TCSS,and in up-regulating the NCV(P<0. 01).Conclusion"Buqi Yixue"needling is effective in improving clinical symptoms and increasing NCV in patients with diabetic peripheral neuropathy.关键词：Diabetic peripheral neuropathy;Numbness and pain;Buqi Yixue needling;Clinical effect449|450|2更新时间：2023-08-10
- 摘要：Objective To investigate the influence of different courses of electroacupuncture treatment on the compliance and therapeutic outcome of patients with cervical type cervical spondylosis.MethodsA total of 60 patients with cervical type cervical spondylosis were randomly divided into 5-time treatment group and 10-time treatment group,with 30 patients in each group. With the same electroacupuncture prescription,the patients in the 5-time treatment group were given electroacupuncture once a day for 5 times in total,and those in the 10-time treatment group were given electroacupuncture once a day for 10 times in total,with an interval of 2 days between every 5 times of treatment. The two groups were compared in terms of cervical Visual Analogue Scale(VAS)score and treatment score of cervical spondylosis before and after treatment,as well as whether and why the patients were willing to continue treatment after 5 times of treatment. The two groups were followed up to observe the recurrence of cervical spondylosis within 12 months.ResultsBoth groups had significant improvements in cervical VAS score and treatment score of cervical spondylosis after treatment(P<0. 05),and there were no significant differences in these scores between the two groups after treatment(P>0. 05). There was also no significant difference in long-term recurrence between the two groups(P>0. 05).Of the 30 patients in the 10-time treatment group,21(70%)showed no willingness to continue treatment after 5 times of treatment since they were satisfied with their treatment outcomes.ConclusionElectroacupuncture can effectively improve the pain symptom of patients with cervical type cervical spondylosis,and compared with 5 times of electroacupuncture treatment,10 times of electroacupuncture treatment may affect patients' compliance with clinical treatment.关键词：Cervical type cervical spondylosis;Electroacupuncture treatment;Marginal utility;Clinical compliance518|532|4更新时间：2023-08-10
- 摘要：Objective To observe the clinical effect of"Yinqi Guiyuan"needling in the treatment of primary insomnia.MethodsA total of 79 primary insomnia outpatients were randomly divided into treatment group(n=40)and control group(n=39). The patients in the control group were given oral Estazolam tablets once a day,for successive 4 weeks. For patients of the treatment group,Zhongwan(CV12),Xiawan(CV10),Qihai(CV6),Guanyuan(CV4),Baihui(GV20),etc.,were punctured with filiform needles for 30 min. The treatment was conducted three times per week for 4 successive weeks. The sleep quality(sleeping quality,falling asleep time,sleep duration,sleep efficiency,sleep disorders,hypnotic and daytime dysfunction,0 to 21 points) was evaluated by using Pittsburgh Sleep Quality Index(PSQI). The severity of insomnia(self-perception,sleep satisfaction,daytime function damage,sensibility change,and concern for sleep problems,0 to 28 points)was assessed using insomnia severity index(ISI)score. The therapeutic effect was evaluated according to the PSQI score reduction rate =(pre-treatment PSQI score—post-treatment PSQI score)/pre-treatment PSQI score ×100%.ResultsAfter treatment,the total score of PSQI,ISI and the score of each item were all significantly reduced in the two groups relevant to their own pre-treatment(P<0. 05). The total score,and scores of hypnotic and daytime dysfunction were significantly lower in the treatment group than in the control group(P<0. 05). Of the 40 and 39 cases in the treatment and control groups,5(12. 50%)and 4(10. 25%)were cured,20(50. 00%)and 18(46. 15%)experienced marked improvement,12(30. 00%)and 13(33. 33%)were effective,and 3(7. 50%)and 4(10. 25%)ineffective,with the total effective rate being 92. 50% and 89. 74%,respectively. No significant difference was found between the two groups in the effective rate(P>0. 05).Conclusion"Yinqi Guiyuan"needling and Estazolam are comparable in treatment primary insomnia,and the former is superior to the latter in avoiding hypnotic drug use and in improving daytime function.关键词：Primary insomnia;"Yinqi Guiyuan" needling;Estazolam459|670|0更新时间：2023-08-10
- 摘要：The effects of acupuncture on functional regulation of biological systems have been studied extensively. The most interesting feature of acupuncture is the bidirectional regulatory ability to restore homeostatic values of body systems from either up or down states,which was found by Chinese biomedical researchers several decades ago,and has recently been observed by peers internationally. Acupuncture can produce two physiological response patterns,an immediate short-term response or/and a long-lasting response. The bidirectional regulatory effects of acupuncture are rarely observed under physiological states(normal homeostasis),rather,they are usually observed under pathological states(abnormal homeostasis)with long-lasting response patterns. This suggests that the effects of acupuncture under pathological states are different from the mechanisms under normal physiological states. This is important for understanding and developing acupuncture therapy,and also gives insight into understanding the biological control process itself.The present paper reviews the experimental literature and discusses the four conditions that produce bidirectional regulation of acupuncture,i. e. the inherent properties of the target systems,the functional states of the target system,the acupoint characteristics,and the stimulation parameters. Finally,two hypotheses are proposed to explain the mechanisms of bidirectional effects of acupuncture. The possible peripheral mechanism is that acupuncture can stimulate different types of peripheral nerve fibers to produce opposite regulatory effects;and the possible central mechanisms is that acupuncture might activate the adaptive control process of the central nervous system to restore homeostatic balance.关键词：Acupuncture;Bidirectional regulation;Homeostasis;Allostasis;Adaptive regulation409|1559|15更新时间：2023-08-10
- 摘要：Bloodletting puncture at twelve well-points is a characteristic emergency therapy in traditional Chinese medicine. This article reviewed the research advances in the clinical effect of this therapy in the treatment of acute central nervous injury and its mechanism of action over the past 30 years,and it is found that this therapy can effectively improve disturbance of consciousness,neurological defects,and cerebral edema caused by stroke,traumatic brain injury,and carbon monoxide poisoning. The mechanism involves the improvement of cerebral blood flow and tissue oxygen supply,repair of the blood-brain barrier,and regulation of local ion balance. Well-designed clinical trials and in-depth research on biological mechanisms should be performed in future to promote and guide its clinical application.关键词：Bloodletting puncture at twelve Jing-well points;Acute central nervous injury;Clinical effect;Mechanism523|994|2更新时间：2023-08-10
- 摘要：In the present paper,we introduce a newly modified device named"transcutaneous electrical auricular concha stimulator(TEACS)"for rats. It concludes a main unit(power and control buttons)with newly designed specific output electrodes. Each of the output electrode is made up of two pieces of iron sheet containing magnet and can be firmly attached to the auricular concha and the corresponding site of dorsal auricle,respectively. The two iron sheets are separately connected to each of the output terminal of the main unit via two pieces of wire. This newly modified output electrode replaces the original wire clip,and solves the problems of easy loosening and easy injury of the original spring clip due to repeated usage,being simple,flexible and convenient in application.关键词：Transcutaneous electrical stimulation;Auricular concha;Experimental device for rats436|246|1更新时间：2023-08-10
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