最新刊期
卷 44 ， 期 6 ， 2019
- 摘要：Objective To observe the effect of electroacupuncture(EA)combined with transplantation of Schwann cells(SCs)on limb locomotor,myelin sheath repair and expression of CD4 and CD8 in compressed spinal cord injury(CSCI)rats,so as to explore its mechanisms underlying improvement of CSCI.Methods A total of 45 female SD rats were randomly divided into normal control,model,EA,Schwann cell(SC)transplantation,and EA+SC transplantation groups(n=9 rats in each group).The CSCI model was established by laminectomy at T12—L2 and clip compression.Rats of the SC transplantation group accepted injection of the cultured SC suspension(2×106/6!L)into the central,upper and lower sites of the injured spinal cord(5 mm in depth)7—8 days after CSCI modeling.EA(2 Hz)was applied to bilateral"Zusanli"(ST36)and"Sanyinjiao"(SP6)for10 min,once daily and 6 days a week for 3 weeks.The Basso,Beattie and Bresnahan locomotor rating scale(BBB scale)was used to evaluate the function state of CSCI.Morphological changes of the regional injured tissue were observed under light microscope after H.E.staining.The myelin sheath repair state and survival of SCs were detected by Luxol fast blue(LFB)staining and immunofluorescence histochemistry,and the expression of CD4,CD8 and P0 of the injured spinal cord was detected by Western blot.Results Compared with the normal control group,the BBB scores at the time-points of 0 d,and 1,2,and3 weeks were significantly decreased in the model group(P<0.001),and those of the EA+SC transplantation group at the 2 nd and 3 rd week were significantly higher than those of the model group(P<0.05).No significant changes of BBB scores were found after EA and SC transplantation relevant to the model group(P>0.05).LFB staining showed a disordered arrangement of the nerve fibers in the white matter,myelinociasis and obvious decrease of the medullated fibers in the model group,and these situations were relatively milder in both EA and SC transplantation groups and obviously milder in the EA+SC transplantation group.H.E.staining displayed that the structure of the injured region of the spinal cord was incomplete,accompanied with a large number of defect cavities and neuronal karyopyknosis in the model group,while the structure was relatively clear,with an increase of the normal neurons and fewer neuronal karyopyknosis in the EA+SC transplantation group.Compared with the normal control group,MBP in the model group was significantly decreased(P<0.001),and P0 was significantly increased(P<0.001).Compared with the model group,the expressions of MBP and P0 were significantly increased in the EA,SC transplantation,and EA+SC transplantation groups(P<0.01,P<0.001),and was significantly higher in the EA+SC transplantation group than in both EA and SC transplantation groups(P<0.001).The average immunofluorescence intensity of Hoechst33342-labeled SCs was significantly higher in the EA+SC transplantation group than in the SC transplantation group(P<0.05).After CSCI,the expression levels of spinal CD4,CD8 and P0 proteins had no significant changes in comparison with the normal control group(P>0.05),while after the intervention and in comparison with the model group,the expression levels of P0 protein were significantly increased in the EA,SC transplantation and EA+SC transplantation groups(P<0.05),and was significantly higher in the EA+SC transplantation group than in both EA and SC transplantation groups(P<0.05).The expression levels of CD4 and CD8 proteins were significantly lower in the EA+SC transplantation group than in the SC transplantation group(P<0.05).Conclusion EA+SCs transplantation can improve the locomotor function in CSCI rats,which may be related to its effects in increasing the survival of transplanted SCs to promote the remyelination and in reducing the immune rejecting reaction.关键词：Compressed spinal cord injury;Electroacupuncture;Schwann cell transplantation;Myelin sheath;Immune rejection547|328|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of manual acupuncture(MA)+ electroacupuncture(EA)on changes of neurological function and expression of monocarboxylate transporter 2(MCT2)in cerebral ischemia(CI)rats,so as to explore its mechanism underlying improvement of ischemic cerebrovascular disease.Methods Eighty male Wistar rats were equally randomized into four groups:normal control(normal),sham operation(sham),model and acupuncture.The CI model was induced by middle cerebral artery occlusion(MCAO)with a thread embolus.Manual acupuncture stimulation(mild twisting reinforcingreducing method)was applied to"Baihui"(GV20)and"Fengfu"(GV16)for 10 min.EA(1 mA,2 Hz/15 Hz)was respectively applied to bilateral"Quchi"(LI11)and"Zusanli"(ST36)for 20 min,once per day for 7 days.The neurological deficit severity was evaluated according to Zea Longa's methods.The activity of lactate dehydrogenase(LDH),fructose-6-phosphate kinase(PFK)and pyruvate kinase(PK)in the peri-ischemic cortex tissue was detected by enzymatic chemistry,and the expression of MCT2 detected by immunofluorescence histochemistry,Western blot and quantitative real-time PCR,separately.Results After CI and in comparison with the normal and sham groups,the Zea Longa's score,the fluorescence intensity and the expression level of MCT2 protein were significantly increased(P<0.01),and the activity of LDH,PFK and PK in the peri-ischemic cortex was significantly decreased in the model group(P<0.01).There was no significant change in the relative expression of MCT2 mRNA(P>0.05).Following the intervention and in comparison with the model group,the Zea Longa's score was considerably decreased in the acupuncture group(P<0.01),the activity of LDH,PFK and PK,and the expression levels of MCT2 protein and mRNA were considerably or further up-regulated in the acupuncture group(P<0.01,P<0.05).Conclusion Acupuncture intervention can improve neurological function in CI rats,which is possibly related with its effects in up-regulating the expression of MCT2 and promoting the utilization of lactate in peri-ischemic cortex.关键词：Acupuncture;Focal cerebral ischemia;Neurological function;Monocarboxylate transporter 2554|336|3更新时间：2023-08-11
- 摘要：Objective To observe the influence of scalp acupuncture on cerebral infarct size and expression of IL-10,IL-6,and IL-1βin the para-hippocampal gyrus in acute ischemic cerebrovascular disease(AICD)rats,so as to investigate its mechanisms underlying improvement of AICD.Methods Forty-eight male SD rats were randomly allocated to normal control(control),AICD model,medication,and scalp acupuncture groups(n=12 per group).The AICD model was established by occlusion of the middle cerebral artery(MCAO).Rats of the medication group received intraperitoneal injection of Ammonium1-Pyrrolidinedithiocarbamate(APDC,100 mg·kg-1·d-1),once daily for 7 days.Scalp acupuncture stimulation was applied to bilateral"Dingnieqianxiexian"(MS6)once daily for 7 days.Before and after intervention,the neurologic deficit score(NDS)and the neurological score(NS)were evaluated according to Longa's and Schbitz's methods,respectively.At the end of the intervention,the para-hippocampal gyrus and whole brain were collected respectively.The expression levels of IL-10,IL-6 and IL-1βin the para-hippocampal gyrus tissue were detected by immunohistochemistry,and the cerebral infarct volume of the brain was detected by triphenyltetrazollium chloride(TTC)staining after sectioning.Results Following modeling,the NDS,NS and the expression of IL-10,IL-6 and IL-1βin para-hippocampal gyrus were significantly increased in the model group compared with the control group(P<0.01).After the intervention,the NDS,NS and infarct volume,and the expression levels of IL-6 and IL-1βin the para-hippocampal gyrus were significantly decreased in both medication and scalp acupuncture groups compared with the model group(P<0.05),and the expression of IL-10 was further obviously up-regulated in the scalp acupuncture group(P<0.05)rather than in the medication group(P>0.05).The effect of scalp acupuncture was obviously superior to that of medication in up-regulating IL-10 expression level(P<0.05).No significant differences were found between the medication and scalp acupuncture groups in the levels of NDS,NS,infarct volume,IL-6 and IL-1βproteins(P>0.05).Conclusion Scalp acupuncture can improve neurological function and reduce infarct volume in AICD rats,which may be associated with its function in up-regulating the expression of IL-10 and in inhibiting the expression of IL-6 and IL-1βto reduce inflammation reaction.关键词：Scalp acupuncture;Ischemic cerebrovascular disease;IL-6;IL-1β;IL-10;Cerebral infarct volume564|485|10更新时间：2023-08-11
- 摘要：Objective To investigate the effect of acupuncture on behavior changes and the expression of reactive oxygen species(ROS),cytochrome C,cysteine-containing aspartate-specific proteases-3(caspase-3),apoptosis inducing factor(AIF)in the hippocampus of rats exposed to chronic restraint stress(CRS),so as to provide experimental evidence for the prevention and treatment of depression.Methods Thirty two male SD rats were equally randomized into control,model,acupuncture and medication(Fluoxetine)groups according to the random number table.The depression model was established by chronic psychological stress stimulation(fasting,water deprivation,chronic restraint combined with solitary raising for 28 days).Manual acupuncture stimulation(2 circles/s)was applied to"Baihui"(GV20),"Yintang"(GV29)and bilateral"Sanyinjiao"(SP6)for2 min,followed by retaining the needles for 20 min.Rats in the medication group were treated by gavage of Fluoxetine(0.18 mg/mL,1 mL/100 g body weight).The treatment was conducted once daily for 28 days.The depression-like behavior was evaluated by using open-field test,sucrose preference test,and body weight.The content of ROS of the right hippocampus tissue was detected by using dichloro-dihydro-fluorescein diacetate(DCFH-DA)assay,and the expression levels of cytochrome C,caspase-3 and AIF proteins of hippocampus were determined by using Western blot.Results After 28 days' stress procedure,the depression rats in the model group showed a significant decrease in the numbers of crossing and rearing of open-field test,sucrose preference index and the body weight compared to the control group(P<0.01).The content of ROS and expression levels of cytochrome C,caspase-3 and AIF in the hippocampus of the model group were significantly increased relevant to the control group(all P<0.01).After the intervention and compared with the model group,the decreased levels of the numbers of crossing and rearing,body weight and sucrose preference index,and the up-regulated levels of content of ROS and expression of cytochrome C,caspase-3 and AIF proteins were all reversed in both acupuncture and medication groups(P<0.01,P<0.05),displaying an anti-depressant effect.The effect of acupuncture was significantly superior to that of medication in up-regulating the numbers of crossing and rearing(P<0.01,P<0.05),and in down-regulating the expression of caspase-3(P<0.01).No significant differences were found between the medication and acupuncture groups in increasing the body weight and sucrose preference index,and in down-regulating the expression of cytochrome C and AIF proteins(P>0.05).Conclusion Acupuncture intervention can significantly reduce ROS content and expression level of cytochrome C,caspase-3 and AIF proteins in the hippocampus possibly by inhibiting the chronic psychological stress—hippocampal oxidative stress—mitochondrial apoptotic pathway in psychological stress-induced depression rats,which may contribute to its effect in relieving depression.关键词：Acupuncture;Depression;Hippocampus;Cell apoptosis;Chronic psychological stress;Oxidative stress578|1135|8更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion on serum adiponectin content,and expression of adiponectin and adiponectin receptor in adipose tissue in Alzheimer's disease(AD)rats,so as to explore its mechanism underlying improvement of AD.Methods Fifty male SD rats were randomly divided into control,model,Shenque(CV8),Zusanli(ST36)and CV8+ST36 groups(n=10 in each group).The AD model was established by intraperitoneal injection of D-galactose(400 mg·kg-1·d-1)for 5 weeks and scopolamine hydrobromide(3 mg·kg-1·d-1)for 2 weeks.Moxibustion was applied to CV8,ST36 and CV8+ST36 respectively for 3 moxa-cones every time,once daily for 5 weeks.Morris water maze tests were used to assess the rats' learning-memory ability.The contents of serum adiponectin were assayed using ELISA,and the expression of adiponectin and adiponectin receptor in the adipose tissue was detected by quantitative real-time PCR and Western blot,separately.Results Following modeling,the average escape latency of Morris water maze tests was significantly prolonged(P<0.05),the content of serum adiponectin and the expression level of adiponectin mRNA in adipose tissue were significantly decreased(P<0.01),the expression of adiponectin receptor protein significantly decreased in the model group relevant to the control group(P<0.01).After the intervention,the average escape latency was significantly shortened(P<0.05),the decreased serum adiponectin content and adiponectin mRNA expression,and the decreased adiponectin receptor protein expression in adipose tissue were all reversed in the 3 treatment groups(P<0.01,P<0.05).No significant differences were found among the three moxibustion groups in the above indexes(P>0.05).Conclusion Moxibustion at CV8,ST36 and CV8+ST36 is effective in up-regulating serum adiponectin content,adiponectin mRNA expression and adiponectin receptor protein expression in adipose tissue,which may provide evidence for clinical election of acupoints.关键词：Alzheimer's disease;Moxibustion;Adipose tissue;Adiponectin;Adiponectin receptor569|489|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of needling "Danzhong"(CV17),"Zhongwan"(CV12),"Qihai"(CV6),"Xuehai"(SP10)and"Zusanli"(ST36)(Triple Energizer Acupuncture Method)on the learning-memory ability and profile of hippocampal differentially-expressed genes and proteins of SAMP8 mice(rapid aging mice),so as to explore its underlying mechanisms in improving Alzheimer's disease(AD).Methods A total of 60 SAMP8 were used as the dementia model and randomly divided into blank control,acupoint and non-acupoint groups(n=20 per group).The 5 acupuncture points and non-acupoints(subcostal region)on the bilateral sides were needled with filiform needles and manipulated manually for 30 sat each one,once daily,6 times a week for 4 weeks.The Morris water maze tests(location navigation tests and space probing trials)were used to evaluate the mouse's learning-memory ability.The hippocampal tissue was extracted to detect differentially expressed genes and proteins related to acupuncture intervention by gene chip and isobaric tags for relative and absolute quantitation(iTRAQ)techniques as well as bioinformatic analysis,separately.The information is analyzed through bioinformatics database tools.Finally,immunofluorescence staining was used to verify the results of microarray analysis.Results Compared with mice of the control and non-acupoint groups,the escape latency of location navigation task of Morris water maze tests on 4 th and 5 th day of training was significantly shortened in mice of the acupoint group,and the duration of stay in the original safe-platform quadrant was significantly increased in the acupoint group(P <0.05).Gene microarray displayed that in comparison with the control group,898 differentially expressed genes were up-regulated,418 genes were down-regulated in the hippocampus of acupoint group.The iTRAQ analysis indicated that in the acupoint and non-acupoint groups,286 and 299 differentially expressed proteins were up-regulated,319 and 179 proteins down-regulated,respectively.Of the 34 terms containing 47 proteins up-regulated by acupoint needling,including intermediate filament,keratin filament,myelin sheath,postsynaptic density,neuron projection were related with neurite and cytoskeleton.While in the non-acupoint group,of the 24 terms were listed by the system,only the myelin sheath involving 11 differentially expressed proteins functions in activities of neurite and cytoskeleton.Immunofluorescence staining of the hippocampal tissue showed that the high-density distribution areas of neurons and neurite fibers were characterized by decentralization and disordering,with the highlighted areas being mainly near the cell body parts in control mice,but in mice of the acupoint group,the highlighted areas at the neurite were relatively dense,the morphology of hippocampal cells was complete,the fiber structure was clear,dense and orderly,and the neurites were closely arranged and in order,indicating an improvement of the distribution and arrangement of nerve fibers after acupuncture.The height of neurite highlight area of the acupoint group was significantly higher than that of the non-acupoint group(P<0.05).Conclusion The "Triple Energizer Acupuncture"of acupoints is able to improve the learning-memory ability in SAMP8,which may be related to its effects in regulating the expression and function of hippocampal genes and proteins related to neurite and cytoskeleton.关键词：Alzheimer's disease;Senescence accelerated mice prone 8;Bioinformatics;Neurite protuberance;Cytoskeleton521|390|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupoint application of herbal paste on symptoms of allergic rhinitis(AR),serum immunoglobulin E(IgE)and transforming growth factor beta 1(TGF-β1)level,and number of nasal eosinophils(EOS)in rats with AR,so as to explore its underlying mechanisms.Methods Forty male Wistar rats were randomly divided into normal control,model,medication and acupoint application groups(n=10 rats per group).The AR model was established by intraperitoneal(i.p.)injection of mixture solution of ovalbumin,aluminum hydroxide and normal saline(once every other day,for7 times),and nasal drip plus spray inhalation of ovalbumin(on the following day of i.p.,once daily for 9 days).For acupoint application,the prepared herbal paste(containing White Mustard Seed,Rhizoma Corydalis,unprocessed Radix Kansui,Herba Asari and ginger juice)was applied to bilateral"Feishu"(BL13),"Pishu"(BL20)and"Shenshu"(BL23)for 2 h,once every other day for 7 times.The rats in the medication group were given Fluticasone Propionate nasal spray daily for 14 days.Scores of nasal itching,sneezing and nasal discharge on the day after modeling and the ending of the intervention were used to evaluate behavioral changes.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of serum IgE and TGF-β1,and the infiltration state of EOS in the nasal mucosa tissue was observed under light microscope after HE staining.Results After modeling and compared with the normal control group,the behavioral scores and the levels of serum IgE and TGF-β1 were significantly higher(P<0.05),and the infiltration state of EOS got worse.Compared with the model group,the increased behavioral score and serum IgE and TGF-β1 levels were evidently suppressed(P<0.05)and EOS infiltration severity in the nasal mucosa was obviously milder in both medication and acupoint application groups.No significant differences were found between the medication and acupoint application groups in behavioral score and serum IgE and TGF-β1 levels(P>0.05).Conclusion Acupoint application can improve the symptoms of AR rats,which may be associated with its effect in down-regulating the levels of serum IgE and TGF-β1.关键词：Acupoint application;Allergic rhinitis;TGF-β1;Inflammatory regulation510|396|1更新时间：2023-08-11
- 摘要：Objective To investigate the efficacy of acupoint injection of Ropivacaine for labor analgesia and its effect on breastfeeding and prolactin secretion.Methods A total of 80 primipara who asked to receive labor analgesia were randomized into epidural analgesia group(n=35)and acupoint injection group(n=36),and other 36 primipara who refused to receive labor analgesia were subjected to the control group.The labor analgesia began to be performed when the puerpera's uterine orifice opened about≥3 cm(the first stage of labor),including puerpera controlled epidural analgesia(PCEA,Sufentainil+ 0.1%Ropivacaine hydrochloride,5 !g/mL at L3—L4 interspace,till the birth of fetus)or acupoint injection of Ropivacaine(0.2%,1 mL/acupoint)at bilateral Hegu(LI4)and Sanyinjiao(SP6).The delivery situations such as the duration of labor,and number of cases who used oxytocin,obstetric-forceps-aided delivery,cesarean delivery were recorded.The serum prolactin concentration was assayed by using ELISA.The visual analogue scale(VAS)scores at the time points of T0(about 3 cm widening of the orifice of uterus and before performing analgesia),T1(30 min after labor analgesia),T2(about 10 cm widening of the orifice of uterus)and T3(coming out of fetal head).The duration of labor,ratio of use of oxytocin,onset time of breastfeeding,and times of breastfeeding within 24 postpartum hours were recorded accordingly.Results The VAS scores at time-points of T1,T2 and T3 were significantly lower in both epidural analgesia and acupoint injection groups in comparison with their own T0 and the control group(P<0.05),and were also considerably higher in the acupoint injection group than in the epidural analgesia group(P<0.05).The duration of the 2 nd stage of labor(from complete opening of the uterus orifice to complete birth of the fetus)was significantly longer,(P<0.05)and the number of oxytocin-using puerpera was obviously bigger in the epidural analgesia group than in the control group(P<0.05).After partum,the 1 st breastfeeding time was obviously earlier and the frequency of breastfeeding notably increased in both epidural analgesia and acupoint injection groups than in the control group(P<0.05),the serum prolactin content was remarkably higher in the acupoint injection group than in the epidural analgesia group(P<0.05).No significant differences were found between the acupoint injection and the control groups in the duration of the 1 st and 2 nd stages of labor,and in the numbers of oxytocin-using puerpera,obstetric forceps-aided birth and cesarean delivery(P>0.05),and between the epidural analgesia group and control group in the serum prolactin levels(P>0.05).Conclusion Injection of Ropivacaine at LI4 and SP6 is effective for labor analgesia and raising prolactin level,and favorable to breastfeeding in the early postpartum period.关键词：Labor analgesia;Labor;Breastfeeding;Serum prolactin478|187|2更新时间：2023-08-11
- 摘要：Objective To compare the clinical efficacy of soft-tissue relaxing needling and electroacupuncture(EA)in the treatment of knee osteoarthritis(KOA),so as to explore a new and more effective therapy for KOA.Methods Forty patients with KOA who met our diagnostic criteria were randomly and equally divided into acupuncture group and soft-tissue relaxing needling(relaxing-needling)group.EA(20 Hz,a tolerable strength and duration of 20 min)was applied to the unilateral Neixiyan(EX-LE5)and Waixiyan(EX-LE5),and manual acupuncture stimulation was applied to Heding(EX-LE2),Xuehai(SP10),Xiyangguan(GB33),Liangqiu(ST34),Yanglingquan(GB34)and Yinlingquan(SP9)on the affected side by using uniform reinforcing-reducing technique.In the relaxing-needling group,after identifying the tender point and nodule-like or stiff-strip-muscle spot at the affected limb by palpation,we used filiform needles to insert into them,then,made a longitudinal separation or point-like pricking.The visual analog scale(VAS)pain score,knee flexion activity(range of motion,ROM),and the knee osteoarthritis severity(Lequesne index,composed of daily living,walking distance and pain)were measured before and after the treatment.The therapeutic effect was assessed by consulting the Guiding Principles for Researching New Drugs of Traditional Chinese Medicine(2002)and Criteria for Diagnosis and Assessment of Therapeutic Effect of Syndromes or Illnesses of Traditional Chinese Medicine(1994).Results After the treatment,the VAS score and Lequesne index were significantly decreased in both acupuncture and relaxingneedling groups(P<0.001),and the ROM score was considerably increased in both groups in comparison with their own pretreatment(P<0.001).The difference values of VAS score and Lequesne index between pre-and post-treatment were significantly higher in the relaxing-needling group than in the acupuncture group(P<0.05).Of the two 20 cases in the relaxing-needling and acupuncture groups,8 and 3 experienced a remarkable improvement in their symptoms,10 and 13 were effective,2 and 4 failed,with the effective rate being 90.0% and 80.0%,respectively.No significant difference was found between the two groups in the difference value of ROM score and the effective rate(P>0.05).Conclusion Both relaxing-needling and EA therapies are comparable in the therapeutic effect for KOA,and the former is superior to the latter in reducing the joint pain and improving the knee joint locomotor function,thus being worthy of clinical application.关键词：Soft tissue relaxing needling;Electroacupuncture;Knee osteoarthritis;Tender-points;Clinical efficacy observation662|509|3更新时间：2023-08-11
- 摘要：Objective To observe the clinical effect of acupuncture stimulation of Sanhuang points in the treatment of chronic nonbacterial prostatitis(CNP)of kidney-yang deficiency type.Methods A total of 60 CNP outpatients were equally randomized into medication(control)group and acupuncture plus medication(acupuncture)group.Patients of the control group were ordered to take Tamsulosin(0.2 mg/d)for successive 8 weeks.On the basis of medication treatment,for patients of the acupuncture group,bilateral Sanhuang points including Tianhuang-fu Point(Shenguan),Dihuang Point and Renhuang Point(at the lower leg)were needled with filiform needles which were manipulated for a while till Deqi,followed by retaining the needles for 30 min.The treatment was conducted once daily for successive 8 weeks.The therapeutic effect was evaluated in reference to the"Criteria for Diagnosis and Therapeutic Effect Evaluation of Syndromes/Diseases of Traditional Chinese Medicine".The National Institutes of Health—Chronic Prostatitis Symptom Index(NIH-CPSI)scores were used to assess the severity of chronic prostatitis.Routine examination of the leukocyte count in the prostatic fluid was performed before and after the treatment.Results Of the two 30 cases of the control and acupuncture groups,9 and 19 were cured,14 and 10 experienced improvement,and 7 and 1 failed in the treatment,with the effective rates being 76.67%and 96.67%,respectively.The effective rate of the acupuncture plus medication was significantly higher than that of the medication(P<0.05).After the treatment,the scores of NIH-CPSI and white blood cell counts in the prostatic fluid in both groups were significantly decreased in comparison with their own pre-treatment(P<0.05).The therapeutic effect of acupuncture plus medication was significantly superior to that of the medication alone in reducing the NIH-CPSI symptom score and the number of leukocytes in the prostatic fluid(P<0.05).Conclusion Acupuncture at Sanhuang points is effective in improving symptoms of CNP patients of kidney-yang deficiency type,and the therapeutic effect of acupuncture plus medication is superior to that of medication alone.关键词：Acupuncture;Chronic nonbacterial prostatitis;Sanhuang points;Kidney-yang deficiency type441|518|6更新时间：2023-08-11
- 摘要：Objective To observe the relationship between the analgesic effect of balance acupuncture and functional changes in brain in patients with migraine without aura.Methods A total of 40 cases of migraine without aura were equally randomized into a headache-acupoint group and a sham-acupoint group.When acupuncture given,a filiform needle was inserted into the headache-acupoint(the midpoint of the depression region anterior to the juncture of the first and second metatarsal bones on the dorsum of the foot)or the sham point(the midpoint of the depression region anterior to the juncture site between the 3 rd and4 th metatarsal joints of the dorsum of the foot)about 25—40 mm deep and manipulated for a while till the patient experienced feelings of electric shock and numbness,then withdrawn immediately.The treatment was conducted once daily for 4 weeks.The visual analogue scale(VAS)was used to evaluate the severity of pain,and the regional homogeneity(ReHo)analysis of resting state functional magnetic resonance imaging(fMRI)was used to assess changes of the spontaneous brain activity.Results After acupuncture,the analgesic effect of headache-acupoint was better than that of the sham-acupoint in both intervention stage and the follow-up stage(P<0.05),and was also stronger in the intervention stage than in the follow-up stage(P<0.05).There was no significant difference in the analgesic effect between the intervention stage and the follow-up stage in the sham-acupoint group(P>0.05).Compared with pre-intervention,4-weeks' intervention at the headache-acupoint showed an increase of ReHo values in the anterior cingulate gyrus,anterior central gyrus,superior orbital frontal gyrus,insula,inferior lobule,left anterior cingulate gyrus,ventral lateral nucleus and ventral posteromedial nucleus of the thalamus,pontine nucleus,cerebellar tonsils and orbital frontal inferior gyrus of the brain(P<0.05),and a decrease of ReHo values in the right brain bridge,central posterior gyrus,posterior cingulate gyrus,left central anterior gyrus,posterolateral nucleus of thalamus,and hippocampus(P<0.05),separately.In the sham-acupoint group,the ReHo value was increased in the right tongue gyrus,the left anterior lobe,the anterior cingulate gyrus and the lower occipital gyrus of the brain(P<0.05),and reduced in the left ventral posterolateral nucleus of the thalamus,separately(P<0.05).Conclusion Balance acupuncture stimulation of headache acupoint has an analgesic effect in migraine patients without aura,which may be related to its effect in regulating resting state brain function of the limbic-system-dominated multiple brain regions.关键词：Balance acupuncture;Migraine without aura;Acupuncture analgesia;Functional magnetic resonance imaging;Regional homogeneity553|696|6更新时间：2023-08-11
- 摘要：Objective To investigate the clinical effect of acupuncture at Sifeng(EX-UE10)combined with Wang's Baochi Pills in the treatment of pediatric malnutrition,so as to provide a more effective method for pediatric malnutrition.Methods A total of 201 children with malnutrition were randomly divided into combined treatment group(n=102)and control(Baochi Pill)group(n=99).The children in the combined treatment group were treated by acupuncture stimulation of Sifeng(EX-UE10,till no more yellowish-white effusion out)and oral administration of Wang's Baochi Pills,and those in the control group treated by oral administration of Wang's Baochi Pills only.The course of treatment was one month for both groups.The integral score of symptom was assessed according to the main symptoms as body weight and height and food-intake,and to the secondary symptoms including mentality,agitation,sleep,hair gloss,susceptibility to cold,hydrosis,abdominal distension,and susceptibility to diarrhea or constipation.The therapeutic effect was assessed by consulting the "Criteria for Diagnosis and Therapeutic Effect Evaluation of Syndromes/Illnesses of Traditional Chinese Medicine(TCM)"and "TCM Professional Criteria of the People's Republic of China for Diagnosis and Therapeutic Effect Evaluation of Syndromes/Diseases of TCM Pediatric".Results After the treatment,the total symptom scores of both groups were significantly decreased in comparison with their own pre-treatment(P<0.05),and the scores of total symptom after the treatment and the 1 st,2 nd and 3 rdfollow-up surveys were obviously lower in the combined treatment group than in the control group(P<0.05).Of the 99 and 102 cases in the control and combined treatment groups,47 and 59 were cured,39 and 37 experienced improvement in their symptoms,and 13 and 6 failed,with the effective rate being 86.87%(86/99)and 94.11%(96/102),respectively.The effective rate of the combined treatment was evidently higher than that of the simple medication(P<0.05).Conclusion Acupuncture of Sifeng(EX-UE10)combined with Wang's Baochi Pills is better than administration of Wang's Baochi Pills alone in the therapeutic effect for pediatric malnutrition.关键词：Pediatric malnutrition;Acupuncture;Sifeng(EX-UE10);Wang's Baochi Pill450|296|0更新时间：2023-08-11
- 摘要：Reinforcing and reducing needling manipulations are important factors affecting clinical therapeutic effect.In the present paper,the relevant elements of the reinforcing and reducing techniques of acupuncture needle including the left-and right-ward twirling,gender,needling at the left and right,front and back parts of the body,needling along or against the running course of the meridian,and their origin and development recorded in ancient Chinese medical books were collected and sorted out,followed by analysis on the understandings of Chinese ancient medical practitioners about them.Results show that the right-or left-ward twirling of needles,gender,and needling at the right or left part,the front or the back part of the body of patients are not the core components of the reinforcing and reducing techniques.Of the three stimulus parameters of needling,named amplitude,frequency and duration which are frequently researched at present,only the duration of single twirling(frequency)was highly noted in GAO Wu's book Zhenjiu Juying(A Collection of Gems in Acupuncture and Moxibustion).It is worthy of being studied in the further.Regarding the stimulation intensity of acupuncture involving the identification of reinforcing or reducing manipulations,the factors influencing the patients' feelings of needling intensity of acupuncture should be studied at the same time.关键词：Acupuncture therapy;Reinforcing and reducing techniques;Acupuncture needle manipulation501|628|4更新时间：2023-08-11
- 摘要：Excessive autophagy is one of the crucial factors of cerebral ischemia-reperfusion injury(CIRI),which has been demonstrated to be one of the targets for acupuncture treatment of ischemic stroke.In the present paper,we make a review about the development of acupuncture intervention induced improvement of CIRI(such as reducing the infarction area,improving learning-memory ability and motor function)by regulating autophagy in animal studies.Outcomes showed that acupuncture intervention can function in 1)inhibiting CIRI-induced increase of the number of lysosomes and autophagic lysosomes,and relieving structural injury of mitochondria,and reducing the number of autophagosome in the central region of the ischemic cerebral cortex tissue;2)down-regulating the expression of microtubule-associated proteinⅡlight chain 3(LC3Ⅱ)and the ratio of LC3-Ⅱ/LC3-Ⅰin the ischemic cerebral region,and 3)regulating the expression of Beclin 1(autophagy-related gene),promoting the expression of P62(autophagy-related adaptor protein).In addition,acupuncture can also regulate phosphoinositide 3 kinase(PI3 K)-protein kinase B(AKT)-mammalian target of rapamycin complex 1(mTOR)signaling at different time-points(down-regulation at the early stage and up-regulation at the later stage),and activating AMP-activated protein kinase(AMPK)-mTOR-UNC51-like kinase-1 signaling to relieve cerebral ischemic injury.These results reveal some mechanisms of acupuncture therapy underlying improvement of CIRI and provide experimental basis for clinical application of acupuncture therapy in the treatment of ischemic stroke.关键词：Acupuncture;Autophagy;Cerebral ischemia-reperfusion injury;Cerebral protection596|916|6更新时间：2023-08-11
- 摘要：Pain is inherently unpleasant and has the characteristic of protecting the body by promoting motivation and learning.Relief from aversion(including pain relief)is rewarding.The aversion to pain and reward from pain relief are encoded by brain mesocorticolimbic circuits characterized by reward/motivation.This paper reviews the research findings about the effect of pain and acupuncture on reward/motivation circuits derived from animal models and human neuroimaging.It is further suggested that neuroimaging techniques may be used to track brain regions that mediate acupuncture effect,and the response of reward/motivation circuits to acupuncture could be used as an objective indicator of analgesic efficacy.It may provide help for further study on the brain mechanism of acupuncture in improving chronic pain.关键词：Reward;Motivation;Pain;Acupuncture443|530|1更新时间：2023-08-11
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