最新刊期
卷 45 ， 期 11 ， 2020
- 摘要：Objective To observe the effect of electroacupuncture(EA) stimulation of "Zusanli"(ST36)on gastric function(food consumption and gastric emptying rate) and excitability of hippocampal glutamatergic neurons in mice, so as to explore its mechanism underlying enhancing gastrointestinal function. Methods The present study includes 2 parts. 1) C57 BL/6 mice were randomly divided into normal and EA groups(n=12 in each group). EA(2 Hz/15 Hz, 1—3 mA) was applied to bilateral ST36 for 20 min, once daily for 7 days. In each group, 6 mice were used to measure the food consumption and gastric emptying rate, and the other 6 mice used to detect the hippocampal glutamate secretion content by using in vivo microdialysis and high performance liquid chromatography. 2) Thirty CaMKIIα-Cre mice received microinjection of a recombinant adeno-associated viral vector containing inhibitory designer receptor exclusively activated by a designer drug(DREADD, AAV-DIO-hM4 Di-eYFP) into the hippocampus. Twenty-one days later, 3 mice were selected to observe the expression of eYFP-labeled hM4 Di by immunohistochemistry, and 15 mice employed to observe the electrical activities of hM4 Di-eYFP positive neurons exposed in chemogenetic activating drug Clozapine N-oxide(CNO) perfusion conditions(n=3) and without CNO in the recording chamber(n=6 in the control and EA groups) by using whole cell patch clamp. The rest 12 CaMKII-Cre mice were equally randomized into AAV-DIO-hM4 Di-eYFP+CNO group and AAV-DIO-hM4 Di-eYFP+CNO+EA group, and CNO was given by intraperitoneal injection for observing the effect of EA on gastric function. Results 1) In C57 BL/6 mice, compared with the normal group, the food consumption, gastric emptying rate, and the glutamate content in the hippocampus were obviously increased in the EA group(P<0.01). 2) In CaMKIIα-Cre mice, the hM4 Di-eYFP positive neurons distributing in the hippocampus showed an obvious increase of firing rates in the EA group relevant to the control group(P<0.01), and a hyperdepolarization potential after application of CNO. No significant changes were found between the AAV-DIO-hM4 Di-eYFP+CNO and AAV-DIO-hM4 Di-eYFP+CNO+EA groups in the food consumption and gastric emptying rate(P>0.05), suggesting an elimination of EA effect after acute DREADD-mediated activation of the CaMKIIα-positive hippocampal excitatory neurons. Conclusion EA at ST36 can promote food intake and gastric emptying in normal mice but not in CaMKIIα-Cre mice with activated hippocampal hM4 Di receptors,suggesting a contribution of the CaMKIIα-positive hippocampal excitatory neurons(glutamatergic neurons in particular) to the enhanced gastrointestinal function of EA at ST36.关键词：Electroacupuncture;Gastric function;Hippocampus;CaMKⅡα-positive excitatory neurons328|707|8更新时间：2023-08-10
- 摘要：Objective To investigate the effect of electroacupuncture(EA) on miR-34 a-5 p, silent mating type information regulation 2 homolog-1(SIRT1) and nuclear factor-κB subunit p65(NF-κB p65) in the trigeminal ganglion of rats with migraine, so as to explore the mechanisms of EA underlying prevention of migraine. Methods Male SD rats were randomly divi-ded into normal, sham operation, model, EA, and EA plus EX527(a SIRT1 inhibitor) groups, with 10 rats in each group. The rat model of migraine was established by electrical stimulation of the trigeminal ganglion. Before modeling, EA was applied at "Waiguan"(TE5) and "Fengchi"(GB20) for 20 min each time, once a day for 5 consecutive days, and intraperitoneal injection of EX527(5 mg/kg) every day simultaneously. Serum prostaglandin E2(PGE2) and calcitonin gene-related peptide(CGRP) concentrations were measured by enzyme-linked immunosorbent assay. The levels of miR-34 a-5 p, SIRT1 and interleukin-1β(IL-1β) mRNA,and protein expression of SIRT1, IL-1β, NF-κB p65, NF-κB Ac-p65 and cyclooxygenase-2(COX2) in trigeminal ganglia were detected by real-time quantitative PCR and Western blot, separately. Results The serum concentrations of PGE2 and CGRP, the expression of miR-34 a-5 p, IL-1β mRNA and protein, NF-κB p65, NF-κB Ac-p65 and COX2 protein expression in the trigeminal ganglion were remarkably increased(P<0.05), while the SIRT1 mRNA and protein decreased(P<0.05) in the model group in contrast to the normal group. Following EA intervention, the serum PGE2 and CGRP concentrations, miR-34 a-5 p expression, IL-1β mRNA and protein, NF-κB p65, NF-κB Ac-p65 and COX2 protein expression were significantly down-regulated(P<0.05), and SIRT1 mRNA and protein significantly up-regulated(P<0.05). Compared with the EA group, the serum concentrations of PGE2 and CGRP, IL-1β mRNA and protein, NF-κB p65, NF-κB Ac-p65 and COX2 protein expressions increased(P<0.05), and SIRT1 protein decreased(P<0.05) in the EA plus EX527 group. Conclusion In migraine rats, EA can inhibit miR-34 a-5 p expression in the trigeminal ganglion, increase SIRT1 expression, down-regulate IL-1β/COX2 inflammation signals, reduce PGE2 synthesis, and thus redue CGRP released from the peripheral terminals, which may be one of the mechanisms of EA in preventing migraine.关键词：Migraine;Electroacupuncture;Trigeminal ganglion;miR-34a-5p;SIRT1;NF-κB p65288|507|4更新时间：2023-08-10
- 摘要：Objective To investigate the effect of electroacupuncture(EA) on gastrointestinal motility and expression of leptin(LEP) and cholecystokinin(CCK) in the small intestine in obese rats,so as to explore the mechanism of EA underlying improvement of obesity. Methods Male Wistar rats were randomized into 5 groups: normal control, obesity model, abdominal acupoints ["Guanyuan"(CV4), "Zhongwan"(CV12) and bilateral "Tianshu"(ST25)], lower-leg acupoints [bilateral "Zusanli"(ST36) and bilateral "Fenglong"(ST40)], and abdominal+ lower-leg acupoints(n=10 rats in each group). The obesity model was established by feeding the animals with high-fat diet for 8 weeks. EA was applied to the abovementioned acupoints for 20 min every time, 3 times a week for 8 weeks. The food intake and body mass were recorded. The white adipose tissue around the testicles and in the abdominal region was weighed. The serum total cholesterol(TC), triglyceride(TG) and non-esterified fatty acid(NEFA) were detected by using automatic biochemical analyzer. The gastric empty rate and intestinal propulsive rate were calculated. The contents of serum CCK and LEP were detected by using ELISA, and the expression levels of CCK and LEP proteins in the small intestine were detected by using Western blot. Results Following modeling, the food intake, body mass, weight of white adipose around the testicles and abdomen, the gastric empty rate, and serum TC, TG, NEFA and LEP contents as well as intestinal LEP expression were significantly increased(P<0.05, P<0.01), while the intestinal propulsive rate, serum CCK content and intestinal CCK expression were evidently decreased(P<0.01) in the model group relevant to the normal control group. After EA interventions and compared with the model group, the increased levels of food intake, body mass, white adipose weight, gastric empty rate, TC, TG, and LEP in serum and small intestine, and the decreased levels of intestinal propulsive rate, CCK in the serum and intestine were reversed in the abdominal acupoints, lower-leg acupoints and abdominal+lower-leg acupoints groups(P<0.05, P<0.01). No significant differences were found in the effects of the three EA groups in down-regulating food intake, body mass, white adipose weight, gastric empty rate, serum TC, TG and LEP levels(except NEFA) and in up-regulating intestinal propulsive rate and CCK level(P>0.05).Conclusion EA stimulation of the abdominal and lower-leg acupoints or both can reduce body weight on obesity rats, which is associated with its functions in regulating intestinal motility, food intake, and secretion of LEP and CCK.关键词：Acupionts compatibility;Electroacupuncture;Obesity;Gastrointestinal motility;Cholecystokinin;Leptin305|527|6更新时间：2023-08-10
- 摘要：Objective To explore the effect of transcutaneous auricular vagus nerve stimulation(taVNS) on blood glucose regulation and the expression of insulin receptors(INR) of hypothalamus, liver and skeletal muscle tissues in impaired glucose tolerance(IGT) rats, so as to reveal its mechanisms underlying improvement of IGT. Methods Thirty-six male Wistar rats were randomly divided into control, model, transcutaneous auricular none-vagus nerve stimulation(tnVNS), and taVNS groups(n=9 in each group). The IGT model was established by feeding the rats with high-fat and high-sugar diet for 5 weeks, and subsequent intraperitoneal injection of a dose of streptozotocin(20 mg/kg). Transcutaneous electrostimulation(2 mA, 2 Hz/15 Hz) was applied to auricular concha(taVNS) or auricular margin(tnVNS), respectively. The treatment was conducted for 30 min once daily for 4 weeks. The body weight, fasting plasma glucose(FPG), 2 h plasma glucose(2 h PG) were recorded every week. The contents of plasma insulin(INS), glucagon(GC), glycosylated hemoglobin(GHbA1 c) were detected by using enzyme linked immunosorbent assay(ELISA). The expression levels of INR in hypothalamus, liver and skeletal muscle tissues were detected by Western blot. Results After modeling, the rats' body weight, the contents of FPG, 2 h PG, GC and GHbA1 c were significantly up-regulated(P<0.001, P<0.05, P<0.01), and the content of INS and expression of INR in hypothalamus, liver and skeletal muscle tissues were significantly down-regulated in the model group compared with the control group(P<0.001, P<0.01, P<0.05). Following the treatment, the increased FPG, 2 h PG, GC, and the decreased INS and INR expression of hypothalamus, liver and skeletal muscle tissues were apparently reversed in the taVNS group relevant to the model group(P<0.001, P<0.01, P<0.05). Compared with the tnVNS group, the FPG and 2 h PG contents were considerable decreased, and the content of INS and INR expression of hypothalamus and liver were obviously increased in the taVNS group(P<0.001, P<0.05, P<0.01). Conclusion taVNS can improve the blood glucose and insulin sensitivity in IGT rats, which may contribute to its effectiveness in up-regulating the expression of INR in hypothalamus, liver and skeletal muscle tissues.关键词：Transcutaneous auricular vagus nerve stimulation;Impaired glucose tolerance;Fasting plasma glucose;2 h plasma glucose;Insulin receptors311|473|2更新时间：2023-08-10
- 摘要：Objective To explore the protective effect of electroacupuncture(EA) on hepatic ischemia-reperfusion injury(HIRI) and the expression of high mobility group protein 1(HMGB1) in liver tissues in rats. Methods A total of 40 male SD rats were randomly divided into 4 groups, namely sham control, HIRI model, "Ganshu"(BL18)-"Yanglingquan"(GB34) and non-acupoint group, with 10 rats in each group. The HIRI model was induced by blocking the arteries, veins and bile ducts supplying the middle and left lobes of the liver for 1 h, and reperfusion for 4 h to induce an area of about 70% HIRI. EA was applied to bila-teral BL18 and GB34, or non-acupoints about 6—8 mm to the bilateral BL18 for 30 min before modeling. Serum alanine transaminase(ALT) and aspartate aminotransferase(AST) levels were measured by using an automatic biochemical analyzer. Serum tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and HMGB1 levels were assayed by ELISA. Hematoxylin-eosin(H.E.) staining was used to observe histopathological changes of the liver tissue by using tissue injury scaling(0-3 scores). The expression of HMGB1 protein in liver tissues was detected by immunohistochemical staining, Western blot and PCR, separately. Results Following modeling and compared with the sham group, the levels of serum ALT, AST, TNF-α, IL-6, and HMGB1 contents, the number of HMGB1 immunoreaction(IR)-positive cells, and HMGB1 protein and mRNA were significantly increased(P<0.01). After the treatment, the contents of serum ALT, AST, TNF-α, IL-6, and HMGB1, liver HMGB1 IR-positive cells, protein and mRNA were considerably down-regulated in the BL18-GB34 group(P<0.05), rather than in the non-acupoint group(P>0.05) in contrast to the model group. H.E. stain showed a higher liver injury score in the model group than in the sham group(P<0.01), and a lower liver injury score in the BL18-GB34 group(not the non-acupoint group) relevant to the model group(P<0.05). Conclusion EA of BL18 and GB34 points has a protective effect on ischemic liver injury in rats with HIRI, which may be associated with its functions in inhibiting the migration and release of HMGB1 from the nucleus to the cytoplasm and in down-regulating the expression of inflammatory factors.关键词：Electroacupuncture;Hepatic ischemia-reperfusion injury;High mobility group protein 1;Inflammatory factor294|276|0更新时间：2023-08-10
- 摘要：Objective To observe the effect of perpendicular and subcutaneous transverse needling at "Sanyinjiao"(SP6) on visceral pain behavior, arginine vasopressin(AVP) content in the serum, uterine tissues, spinal cord and hypothalamus and expression of AVP receptors AVPR1 A and AVPR1 B in the uterine tissues, spinal cord and hypothalamus in cold-stasis(stasis due to pathogenic cold) type dysmenorrhea rats, so as to explore their mechanisms underlying pain relief. Methods Forty female SD rats were randomly divided into blank control, model, perpendicular needling and transverse needling groups, with 10 rats in each group. The cold-stasis dysmenorrhea rat model was established by exposure in a freezer(-25 ℃) for 4 h, once daily for 5 days, and subcutaneous injection of estradiol benzoate(once daily for 10 days) and intra-abdominal injection of oxytocin injection(once). For rats of the two acupuncture groups, acupuncture needles were inserted into the bilateral SP6 perpendicularly or transversely to a depth of about 4-5 mm, and retained for 20 min. The abdominal pain behavior was assessed by recording the writhing latency and scaling the rats' writhing reactions after modeling. The contents of AVP in the serum, uterus, spinal cord and hypothalamus tissues were assayed using ELISA and the expression of AVPR1 A and AVPR1 B in the uterus, spinal cord and hypothalamus was measured by using Western blot and quantitative real time-PCR, respectively. Results After mode-ling and compared with the blank control group, the writhing latency was significantly shortened(P<0.05), and the writhing score in the first 20 min was significantly increased(P<0.01) in the model group. After the intervention, the writhing latency was significantly prolonged(P<0.01), and the writhing scores in 20 min were significantly decreased(P<0.01) in the two needling groups. The AVP contents were obviously increased in the serum and uterine tissue(P<0.05, P<0.01) but decreased appa-rently in the spinal cord and hypothalamus tissues(P<0.01, P<0.05), and the expression levels of AVPR1 A or AVPR1 B protein and mRNA were markedly increased in the uterine tissues(P<0.01, P<0.05), and significantly decreased in the spinal cord and hypotha-lamus in the model group relevant to the control group(P<0.05, P<0.01). Following the intervention, The AVP content in the serum of the perpendicular needling group(P<0.05) and that in the uterus of the two needling groups were significantly decreased(P<0.01), as well as that in the hypothalamus was obviously increased in the two needling groups(P<0.05). The expression levels of AVPR1 A protein and mRNA in the uterus were significantly down-regulated in the two needling groups(P<0.01, P<0.05) and AVPR1 B protein in the hypothalamus of the perpendicular needling group was up-regulated(P<0.05). Moreover, no significant differences were found between the two needling groups in regulating the related indexes mentioned above(P>0.05).Conclusion Both perpendicular and subcutaneous transverse needling at SP6 have an immediate analgesic effect in cold-stasis type dysmenorrhea rats, which may be related to their effects in regulating AVP levels and its receptor expression in the uterine and hypothalamus.关键词：Perpendicular needling;Transverse needling;Arginine vasopressin;Arginine vasopressin receptors;Dysmenorrhea250|462|4更新时间：2023-08-10
- 摘要：Objective To observe and compare the ameliorate effects of electroacupuncture(EA) and transcutaneous electrical acupoint stimulation(TEAS) with different intensities on inflammatory muscle pain, so as to confirm the role of different afferent nerve fibers in different layers(skin or muscle) in pain relief of acupuncture. Methods The intensities of the thresholds of A(Ta) and C(Tc) were selected for TEAS and EA. TEAS and EA were applied on the layer of skin and muscle of "Liangqiu"(ST34), respectively. Forty-eight rats were randomly divided into control, model, TEAS-Ta, TEAS-Tc, EA-Ta and EA-Tc groups(n=8 in each group). The inflammatory muscle pain model was established by complete Freund's adjuvant(CFA) injection into the right biceps femoris muscle. EA or TEAS was applied once a day for 3 days. The weight difference between the hind limbs and the abnormal electromyography(EMG) activities were observed as an index for pain of the rats. Results After modeling, the weight difference between the hind limbs was increased markedly in the model group relevant to the control group(P<0.01). Compared with the model group, the weight difference between the hind limbs in the TEAS-Tc, EA-Ta, and EA-Tc groups was significantly decreased(P<0.05, P<0.01). Compared with that before intervention, the area under the curve and discharge frequency of abnormal EMG of rats in the TEAS-Tc and EA-Ta groups were significantly reduced after intervention(P<0.01, P<0.05), while those in the EA-Tc group were significantly increased(P<0.05). After the intervention, compared with the TEAS-Ta group, the inhibition rate of the area under the curve and the discharge frequency of the abnormal EMG in the TEAS-Tc group increased significantly(P<0.05); and those were also increased in the EA-Ta group when compared with the EA-Tc group(P<0.01). Conclusion TEAS with Tc or EA with Ta in the muscle layer can alleviate the pain and inhibit the abnormal EMG in inflammatory muscle pain rats. It is indicated that local pain relief by acupuncture was related to the afferent nerve fiber in different layer with different intensities.关键词：Inflammatory muscle pain;Electroacupuncture;Transcutaneous electrical acupoint stimulation;Electromyography346|975|12更新时间：2023-08-10
- 摘要：Objective To investigate the effect of electroacupuncture(EA) on pain behaviors and expression of spinal transcription factor GATA-binding Protein 4(GATA4) and adenosine A1 receptor in neuropathic pain rats, so as to explore its mechanism underlying pain relief.Methods The present study includes 2 parts. In the first part, 18 SD rats were randomly divided into control, adenovirus short-hairpin interference RNA for GATA4(AV-shGATA4 RNA) and adenovirus empty vector(AV-control short-hairpin RNA, AV-shCTRL) groups, with 6 rats in each group. The expression of GATA4 protein in the lumbar spinal cord(L4—L6) was detected to evaluate the transfection efficiency of AV-shGATA4 RNA(silencing GATA4 expression). In the second part, thirty SD rats were randomly divided into 5 groups, namely sham operation, CCI model, EA, EA+AV-shGATA4 RNA, and EA+AV-shCTRL groups, with 6 rats in each group. The neuropathic pain model was established by chronic constriction injury(CCI) of the right sciatic nerve. On the 7 th day following modeling, EA was applied to the right "Zusanli"(ST36) and "Taichong"(LR3)(1 mA,2 Hz/100 Hz) for 30 min. Rats of the EA+AV-shGATA4 RNA and EA+AV-shCTRL groups received intrathecal injection of AV-shGATA4 RNA and AV-shCTRL(1×10(11) PFU/mL,10 μL)at the spinal L4—L6 segments, separately, 48 h before EA intervention. The mechanical pain threshold and thermal pain threshold of the affected limb were detected before molding, 7 days following molding and 60 min after EA. The expressions of adenosine A1 receptor and GATA4 protein in the spinal cord(L4—L6) were detected by Western blot.Results Outcomes of the first part showed that compared with the control group, no significant changes were found in the mechanical and thermal pain thresholds in both AV-shCTRL and AV-shGATA4 RNA groups and in the expression of spinal GATA4 protein of the AV-shCTRL group(P>0.05). The expression of spinal GATA4 protein of the AV-shGATA4 RNA group was significantly lower than that of the AV-shCTRL group(P<0.05). In the second part of the study, before CCI modeling, there were no significant differences among the five groups in the mechanical and thermal pain thre-sholds(P> 0.05). On the 7 th day following modeling, the mechanical and thermal pain thresholds were significantly lowered in compa-rison with their own pre-modeling of each group and with the sham operation group(P<0.05). At 60 min after EA and compared with the model group, the mechanical and thermal pain thresholds were significantly increased in both the EA and EA+AV-shCTRL groups(P<0.05) but not in the EA+AV-shGATA4 RNA group(P>0.05), suggesting a critical involvement of GATA4 in EA analgesia. The expression levels of adenosine A1 receptor and GATA4 protein were significantly increased in the model group than in the sham operation group(P<0.05), and considerably further up-regulated in both EA and EA+AV-shCTRL groups(P< 0.05), rather than in the EA+AV-shGATA4 RNA group(P>0.05), suggesting that the effects of EA in up-regulating the expression of A1 receptor and GATA4 were eliminated after silencing GATA4 protein. Conclusion EA of ST36 and LR3 can relieve pain by increasing the expression of adenosine A1 receptor of the lumbar spinal cord in neuropathic pain rats, which is probably mediated by GATA4 protein.关键词：Neuropathic pain;Electroacupuncture analgesia;Adenosine A1 receptor;Transcription factor GATA4262|182|2更新时间：2023-08-10
- 摘要：Objective To observe the effect of acupuncture on nuclear factor kappa-B(NF-κB)signaling pathway in patients with rheumatoid arthritis(RA), so as to explore the mechanism of acupuncture in the treatment of RA. Methods A total of 120 RA patients admitted to the Xinyu Hospital of Traditional Chinese Medicine in Jiangxi Province from June 2018 to June 2019 were selected and randomly divided into a control group and a treatment group, with 60 cases in each group. The patients in the control group received oral administration of Methotrexate,(10-15) mg/time, once a week, for a total of 10 weeks. On the basis of oral administration of Methotrexate, patients in the treatment group were treated with acupuncture at Quchi(LI11), Zusanli(ST36), Taichong(LR3), Hegu(LI4), Shenshu(BL23), Geshu(BL17) and Dazhui(GV14) once daily. The treatment was conducted 6 consecutive times as a course for 10 courses. The serum C-reactive protein(CRP) and erythrocyte sedimentation rate(ESR), pain visual analog scale(VAS) score, number of swollen joints, and morning stiffness time before and after treatment were compared between the two groups. The expression levels of inflammatory related factors and NF-κB p65 protein in the synovial fluid were detected by enzyme-linked immunosorbent assay and Western blot separately. Results After treatment, both groups had significant decrease in CRP, ESR, VAS score, number of swollen joints and morning stiffness time, levels of NF-κB p65 protein, tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and IL-6(P<0.05) in the joint fluid, and increase in transforming growth factor-β(TGF-β) and IL-10(P<0.05). After treatment, compared with the control group, CRP, ESR, VAS score and morning stiffness time, and the levels of NF-κB p65, TNF-α, IL-1β and IL-6 in the treatment group were down-regulated(P<0.05), while the level of TGF-β was up-regulated(P<0.05). The treatment group had a higher total effective rate than the control group[85.0%(51/60) vs 75.0%(45/60), P<0.05]. Conclusion Acupuncture can inhibit the expression of NF-κB signaling pathway in RA patients, regulate the levels of related inflammatory factors, and thus improve the symptoms of RA patients.关键词：Acupuncture;Rheumatoid arthritis;NF-κB signaling pathway;Inflammatory factor265|577|15更新时间：2023-08-10
- 摘要：Objective To observe the effect of transcutaneous electrical acupoint stimulation(TEAS) of different acupoint combinations on postoperative nausea and vomiting(PONV) and serum motilin(MTL) content in patients undergoing laparoscopic surgery, so as to provide evidence for clinical selection of suitable acupoint compatibility. Methods A total of 140 patients undergoing elective laparoscopic surgery were randomly divided into general anesthesia(GA) control, TG_2 [Neiguan(PC6) + Hegu(LI4)], TG_3 [PC6 + LI4 + Zusanli(ST36)], and TG_4 [PC6 + LI4 + ST36 + Sanyinjiao(SP6)] groups, with 35 cases in each group. Patients of the TG_2, TG_3 and TG_4 groups received TEAS(2 Hz/100 Hz, 3—8 mA) of the above mentioned acupoint(bilateral) groups for 30 min before the induction of anesthesia until the end of the operation. Patients of the GA control group received intravenous injection of Midazolam, Sufentanil, Propofol, Rocuronium Bromide, etc. The blood sample(4 mL) from the right median cubital vein was collected at the time of patient's entry, 12 and 24 h after surgery, respectively, for measuring MTL concentration by enzyme-linked immunosorbent assay. The incidence of early and late PONV and changes of PONV degree within 24 h after surgery were recorded. Results The serum MTL concentrations were significantly decreased at 12 h after surgery in all the 4 groups(P<0.05), and decreased at 24 h after surgery in GA and TG_2 groups vs their own pre-surgery(P < 0.05), and considerably higher at both 12 and 24 h after surgery in the TG_2, TG_3 and TG_4 groups than in the GA control group(P<0.05), and also evidently higher in the TG_3 and TG_4 groups than in the TG_2 group(P<0.05). The incidence of PONV was significantly lower in the TG_2, TG_3 and TG_4 groups than in the GA control group in the early and late periods(except TG_2 group) of surgery(P<0.05). The number of patients with PONV grade Ⅰ was significantly larger in the TG_2, TG_3 and TG_4 groups than in the GA control group at 24 h after surgery(P<0.05), suggesting a mild PONV in more patients undergoing TEAS. Conclusion TEAS has a preventive effect on PONV in patients undergoing laparoscopic surgery, which may be related to its effect in reducing serum MTL concentration. The preventive effect of TEAS of PC6 + LI4 + ST36 and PC6 +LI4 + ST36 + SP6 is similar and better than TG_2(two acupoints) group.关键词：Transcutaneous electrical acupoint stimulation;Laparoscopic surgery;Postoperative nausea and vomiting;Serum motilin;Acupoint combination297|722|10更新时间：2023-08-10
- 摘要：Objective To observe the efficacy of "kidney three-needle" therapy[Qugu(CV2) and 1 cun to the left and right of CV2] and the pricking technique of fire needing therapy on urinary frequency and maximal bladder volume in potients with post-stroke urinary incontinence. Methods A total of 60 patients with post-stroke urinary incontinence were collected according to the inclusion and exclusion criteria and were randomized into an observation group(30 cases) and a control group(30 cases). On the base of the routine medication and rehabilitation treatment, acupuncture was applied to the general acupoints for motor impairment and speech disorder in the two groups. Additionally, the combination of "kidney three-needle" therapy and the pricking technique of fire needling therapy was used for urinary incontinence in the observation group and the common acupuncture was applied in the control group. Needles were retained for 30 min and acupuncture was provided once daily, 5 days a weeks, 2 weeks of treatment as one course. Both groups were treated for 3 courses. Before and after treatment, the rank of daytime urinary frequency was compared in the patients of the two groups. Ultrasound B was adopted to determine the maximal bladder volume. The total effective rate was compared between the two groups. Results After treatment, the rank of daytime urinary frequency all reduced significantly as compared with that before treatment in the patients of the two groups(P<0.01), and the observation group was lower than the control group(P<0.05). After the treatment, the maximal bladder volume increased significantly as compared with that before treatment in the self-comparison of each group(P<0.01) and the volume in the observation group was significantly higher than that in the control group(P<0.01). The total effective rate was 93.3%(28/30) in the observation group and was 66.7%(20/30) in the control group. The total effective rate in the observation group was higher obviously than that in the control group(P<0.05). Conclusion The combination of "kidney three-needle" therapy and the pricking technique of fire needling therapy significantly reduces the rank of daytime urinary frequency and increases maximal bladder volume in the patients with post-stroke urinary incontinence. The therapeutic effect of such combined acupuncture treatment is better than the common acupuncture therapy.关键词："Kidney three-needle" therapy;Fire needling therapy;Post-stroke urinary incontinence265|373|1更新时间：2023-08-10
- 摘要：Objective To investigate the methods for locating and selecting the acupoints of "Taixi"(KI3), "Shuiquan"(KI5), "Fuliu"(KI7), "Jiaoxin"(KI8), "Zhubin"(KI9), and "Yingu"(KI10) and the morphological structure of these acupoints in rabbits. Methods According to the WHO and national standards for human acupoints and rabbit X-ray images, acupoint locations were marked using the anatomical landmarks on body surface in 10 New Zealand rabbits. The acupoints were dissected to compare the homologous and analogous tissue between rabbits and human body and thus correct the locations of these acupoints. Potentials were measured for the 10 New Zealand rabbits at the corrected locations of the acupoints and around the acupoints, and the final locations of these acupoints were determined by comparing the anatomical results and the data of potentials. Anatomical observation was performed after marking, and the relationship between acupuncture needle and adjacent structure was observed. Results "Taixi" was located in the ankle area, at the midpoint between the prominence of the medial malleolus and the calca-neal tendon; "Shuiquan" was located in the calcaneal area below "Taixi" in the depression anterior to the calcaneal tuberosity; "Fuliu" was located at the medial side of the calf, at 2 cun above the prominence of the medial malleolus anterior to the calcaneal tendon; "Jiaoxin" was located at the medial side of the calf, at 2 cun above the prominence of the medial malleolus and in the depression posterior to the medial border of the tibia; "Zhubin" was located at the medial side of the calf, at 5 cun above the medial malleolus on the line between "Taixi" and "Yingu"; "Yingu" was located at the medial side of the knee, at the posterior-inferior border of the semitendinosus tendon on the popliteal crease. The results of skin potentials at the acupoints suggested that "Taixi", "Shuiquan", "Fuliu", and "Zhubin" were high-reliability acupoints, "Jiaoxin" was a medium-reliability acupoint, and "Yingu" was a low-reliability acupoint. Conclusion Comparative anatomy combined with imaging, surface anatomy, and electrophysiological techniques of acupoints can help with the accurate localization and selection of acupoints in experimental animals, improve the reliability of acupoint location, and enrich the comparative anatomical data of acupoints.关键词：Acupoint location;Comparative anatomy;X-ray images;Rabbit231|318|0更新时间：2023-08-10
- 摘要：Traditional moxibustion treatment mainly relies on doctors using specific techniques to stimulate the patient's acupoints with ignited moxa sticks. In view of the poor reproducibility, and different methods of different doctors, difficult to carry out quantitative and qualitative research work in clinical research, a moxibustion mechanical arm was designed. The hardware modules of the mechanical arm are composed of power, micro controller STM32, position servos, moxibustion strip thruster, human-computer interaction panel and sensors; the software parts are composed of main control program and interrupt service program. The use of this moxibustion mechanical arm will enhance the system's multi-task adaptability and could perform a variety of moxibustion methods including circling moxibustion and sparrow-pecking moxibustion. The data collected in real time will be transmitted to PC through bluetooth, displayed on OLED, and the system operation modes could be switched by button. Clinical trials showed that moxibustion mechanical arm had the same treatment effects with traditional moxibustion. Meanwhile, its convenience of ope-ration, repeatability of treatment, doctors and patients' s treatment experience are all better than those of traditional moxibustion.关键词：Moxibustion mechanical arm;Circling moxibustion;Sparrow-pecking moxibustion;Micro controller STM32230|545|4更新时间：2023-08-10
- 摘要：Axon guidance cues includes Slit, Semaphorin, Ephrin and Netrin. They have the function of regulating the regeneration of axons and guiding the regenerated axons to the correct target. They can affect the nervous system, cardiovascular system, and participate in the proliferation and migration of tumor cells. The current research on the influence of acupuncture and moxibustion(mainly focusing on electroacupuncture) on axon guidance cues is limited to animal experiments. Electroacupuncture can treat diseases dominated by the nervous system by regulating the expression of axon guidance cues. This review summarizes the research progress of acupuncture and moxibustion on regulating axonal guidance cues, is hoped to provide references for the mechanism of acupuncture and moxibustion in treating nervous system disease and ideas for acupuncture treatment of diseases related to axon guidance cues.关键词：Acupuncture and moxibustion;Axon guidance cues;Review265|451|1更新时间：2023-08-10
- 摘要：In addition to the prone position or the supine position, some scholars have observed and discussed the therapeutic effect of acupuncture in other body positions, but their criteria are different and individual differences in body position have not been analyzed. According to the relevant discussions in The Inner Canon of Yellow Emperor and with reference to the clinical practice of acupuncture treatment, this article explores the criteria for body position selection, in order to provide guidance for clinical acupuncture treatment.关键词：Acupuncture and moxibustion;Position;Review237|399|2更新时间：2023-08-10
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