最新刊期
卷 45 ， 期 3 ， 2020
- 摘要：Objective To observe the effect of electroacupuncture(EA) at "Zusanli"(ST36) and "Feishu"(BL13) on M1 polarization of alveolar macrophages(AM) in rats with chronic obstructive pulmonary disease(COPD), so as to explore its anti-inflammatory mechanism underlying improvement of COPD. Methods Forty SD rats were randomly divided into normal and normal+EA, COPD model and COPD+EA groups(n=10 in each group). The COPD model was established by simple fumigation. EA(4 Hz/20 Hz, 1 to 2 mA) was applied to bilateral ST36 and BL13 for 30 min, once every other day for 2 weeks. The pulmonary function including the forced vital capacity(FVC), forced expiratory volume in 0.1 and 0.3 s(FEV0.1, FEV0.3, FEV0.1/FVC, and FEV0.3/FVC) was detected by using a small animal respiratory function detector. Histopathological changes of the lung were displayed by H.E. staining. The contents of tumor necrosis factor-α(TNF-α) and induced nitric oxide synthase(iNOS) in the broncho alveolar lavage fluid(BALF) were assayed by ELISA. The expression of M1 polarization markers(CD86,iNOS), myeloid differentiation factor 88(MyD88) and nuclear factor-κB p65(NF-κB p65) in AM were detected by Western blot and quantitative real time-PCR, separately. The distribution and expression of CD86 in the lung were detected by immunohistochemistry. Results Following modeling, the levels of FVC, FEV0.1, FEV0.3, ratios of FVE0.1/FVC and FEV0.3/FVC were significantly decreased(P<0.01), while the contents of TNF-α and iNOS in the BALF, expression of CD86, iNOS, MyD88 and NF-κB p65 mRNAs and proteins in the AM, and CD86 immunoactivity in the lung were significantly increased in the model group relevant to the normal group(P<0.01). After the intervention, the decrease of the lung function and increase of the above-mentioned genes and proteins were all reversed in the COPD+EA group(P<0.05, P<0.01). Conclusion EA at ST36 and BL13 can reduce pulmonary inflammation in COPD rats, which may be related to its function in inhibiting M1 polarization of AM via down-regulating MyD88/NF-κB p65 signaling pathway.关键词：Electroacupuncture;Chronic obstructive pulmonary disease;Alveolar macrophages;M1 polarization;MyD88/NF-κB p65 signaling pathway272|486|13更新时间：2023-08-11
- 摘要：Objective To investigate the protective effect of electroacupuncture(EA) on rats with ulcerative colitis(UC) and its effect on the Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor-kappa B(NF-κB) signaling pathway. Methods Sprague-Dawley rats were randomly divided into blank group, model group, salicylazosulfapyridine(SASP) group, low-intensity EA group, and high-intensity EA group, with 8 rats in each group. Enema with 2,4,6-trinitrobenzenesulfonic acid was performed to establish a model of UC. The rats in the two EA groups were given EA at"Tianshu"(ST25), "Guanyuan"(CV4), and "Zusanli"(ST36) for 15 min each time, once a day, with a current intensity of 1 mA for the low-intensity EA group and 5 mA for the high-intensity EA group(among them, "Tianshu" "Zusanli" bilateral alternate acupoints); the rats in the SASP group were given SASP suspension 3 mL every day by gavage. The course of treatment was 15 days for all groups. HE staining was used to observe the pathology of the colon and determine tissue damage index(TDI); ELISA was used to measure the serum levels of interleukin-4(IL-4), interleukin-10(IL-10), interleukin-17(IL-17), and prostaglandin E_2(PGE_2); immunohistochemical staining and real-time PCR were used to measure the protein and mRNA expression of TLR4, MyD88, and NF-κB in colonic tissue. Results Compared with the blank group, the model group had significant reductions in body weight, serum IL-4, and IL-10(P<0.05) and significant increases in colonic mucosa TID, the serum levels of IL-17 and PGE_2 and the protein and mRNA expression of TLR4, MyD88, and NF-κB in colonic tissue(P<0.05). Compared with the model group, the SASP group and the EA groups had significantly higher body weight and serum levels of IL-4 and IL-10(P<0.05), as well as significantly lower colonic TDI, serum levels of IL-17 and PGE_2, and integrated optical density and mRNA expression of TLR4, MyD88, and NF-κB in colonic tissue(P<0.05). Compared with the SASP group, the low-intensity EA group had significantly higher colonic TDI and protein and mRNA expression of TLR4, MyD88, and NF-κB in colonic tissue(P<0.05), and compared with the SASP group, the high-intensity EA group had a significantly higher body weight(P<0.05) and lower colonic TDI and protein and mRNA expression of TLR4, MyD88, and NF-κB in colonic tissue(P<0.05). Compared with the low-intensity EA group, the high-intensity EA group had significantly higher body weight(P<0.05), and lower colonic TDI and protein and mRNA expression of TLR4, MyD88, and NF-kB in colonic tissue(P<0.05). Conclusion Electroacupuncture exerts a protective effect on the colonic mucosa in rats with UC possibly by inhibiting the TLR4/MyD88/NF-κB signaling pathway and reducing the release of inflammatory cytokines, and high-intensity EA may have a better effect than low-intensity EA.关键词：Electroacupuncture;Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-kappa B signaling pathway;Inflammatory cytokine;Ulcerative colitis303|542|9更新时间：2023-08-11
- 摘要：Objective To observe the effect of medicated thread moxibustion of Zhuang Minority medicine on helper T cell 17(Th17)/Interleukin-17 F(IL-17 F) signaling pathway in ulcerative colitis(UC) rats, so as to explore its mechanisms underlying improvement of UC. Methods Forty male SD rats were randomly divided into normal control, model, medication and medicated thread moxibustion(MTM) groups, with 10 rats in each group. The UC model was induced by free drinking of 4% dextran sulfate sodium(DSS) for 10 d. After successful modeling, rats of the medication group were treated by gavage of salazosulfapyridine(SASP). Medicated thread moxibustion was applied to unilateral "Tianshu"(ST25) and "Qihai"(CV6) alternatively for rats of the MTM group, once daily for 14 d. The body mass, stool shape, and fecal occult test were recorded and conducted daily to perform disease activity index(DAI) score. H.E. staining was used to display pathological changes of the colon tissue. The Th17 cells and IL-17 F and retinoic acid related orphan receptor γ t(ROR-γt) in the colon tissue were detected by flow cytometry, and enzyme-linked immunosorbent assay(ELISA), respectively, and the expression levels of RORγt and IL-17 F mRNA in colon tissue were detected by quantitive real-time PCR. Results After modeling, the DAI score, colonic Th17 percentage, RORγt and IL-17 F contents, and RORγt and IL-17 F mRNA expression were significantly increased in the model group in contrast to the normal control group(P<0.01, P<0.05). Following the intervention, all the aforementioned indexes were reversed in both medication and MTM groups(P<0.01, P<0.05). No significant differences were found between the medication and MTM groups in the levels of the above mentioned indexes(P>0.05, except RORγt and IL-17 F mRNA expression). H.E. staining showed disappearance of goblet cells, infiltration of a large number of inflammatory cells, exfoliation of the epithelial tissue and edema of colonic mucosal in rats of the model group, which was relatively milder in both medication and MTM groups. Conclusion Medicated thread moxibustion of Zhuang Minority medicine can reduce the inflammatory damage of colon tissue in UC rats, which is associated with its effects in suppressing the expression of RORγt, production of Th17 cells, and secretion of pro-inflammatory factor IL-17 F in colon tissue.关键词：Ulcerative colitis;Medicated thread moxibustion of Zhuang Minority medicine;Disease activity index;Helper T cell 17;Interleukin-17F308|370|5更新时间：2023-08-11
- 摘要：Objective To investigate the effect of electroacupuncture on the P35/P25-cyclin-dependent kinase 5(CDK5)-Tau pathway in rats with Alzheimer's disease(AD), as well as the mechanism of electroacupuncture in the prevention and treatment of AD. Methods Sprague-Dawley rats were randomly divided into control group, sham-operation group, model group, and electroacupuncture treatment group, with 12 rats in each group. A rat model of AD was established by injection of Aβ_(25-35) into the bilateral hippocampus. The rats in the electroacupuncture treatment group were given electroacupuncture at "Baihui"(GV20) and "Shenshu"(BL23) once a day, 15 min each time, for 10 days. Morris water maze was used to evaluate learning and memory abilities, immunohistochemistry was used to measure the distribution and expression of P35/P25, CDK5, and Tau5 in the hippocampus, and Western blot was used to measure the expression of the above mentioned proteins, phosphory-lated Tau(Ser199, Ser202). Results In the visual platform test, there were no significant differences in escape latency and search path between groups(P>0.05). In the hidden platform test, there were no significant differences in escape latency and search path between the control group and the sham-operation group(P>0.05); the model group had significantly longer escape latency and search path than the control group and the sham-operation group(P<0.05). Compared with the model group, the electroacupuncture treatment group had significantly shorter escape latency and search path(P<0.05). In the spatial exploration test, there was no significant difference in the number of platform crossings between the control group and the sham-operation group(P<0.05). The model group had a significantly lower number of platform crossings than the control group and the sham-operation group(P<0.01, P<0.05). The electroacupuncture treatment group had a significantly higher number of platform crossings than the model group(P<0.05). Compared with the control group and the sham-operation group, the model group had significant increases in the protein expression of P35/P25 and CDK5(P<0.001), and the electroacupuncture treatment group had significant reductions compared with the model group(P<0.001). There was no significant difference in the protein expression of Tau5 between groups(P>0.05). The model group had significantly higher protein expression of phosphorylated Tau(Ser199, Ser202) in the hippocampus than the control group and the sham-operation group(P<0.01, P<0.05). The electroacupuncture treatment group had significantly lower protein expression of phosphorylated Tau(Ser199,Ser202) than the model group(P<0.05, P<0.01). Conclusion Electroacupuncture may delay the progression of AD by affecting the expression of proteins involved in the P35/P25-CDK5-Tau pathway in the hippocampus of rats.关键词：Electroacupuncture;Alzheimer's disease;Hippocampus;P35/P25-cyclin-dependent kinase 5-Tau pathway258|334|4更新时间：2023-08-11
- 摘要：Objective To explore the mechanism of electroacupuncture(EA) underlying improvement of cerebral infarction(CI) by investigating its influence on expression of cerebral Wnt7 a, lymphoid enhancer factor-1(LEF1), glycogen synthase kinase 3β(GSK-3β) and Dickkopf-1(DKK1) mRNA and proteins in CI rats. Methods A total of 280 male Wistar rats were randomly divided into blank control(n=10), sham-operation, model and EA groups,and 90 rats of the last 3 groups were further divided into 1, 3, 6, 9, 12 and 24 h, and 3, 7 and 12 d subgroups with 10 rats in each subgroup. The CI model was established by occlusion of the middle cerebral artery(MCAO). The sham-operation group received the same surgical operation but without thread embolus insertion. EA(2 Hz/15 Hz, 2 mA) was applied to "Shuigou"(GV26) for 20 min, once a day for 1, 3, 7 and 12 d, respectively. The neurological deficit was evaluated by using Neurological Severity Scores(NSS). The expression levels of Wnt7 a,LEF1, GSK-3β and DKK1 mRNAs and proteins in the right ischemic brain tissues were detected by Quantative real-time PCR and Western blot, respectively. Results After MCAO, the NSS score was significantly increased in the model and EA groups relevant to the blank control and sham-operation groups(P<0.01) and gradually decreased with the prolongation of ischemia time. After EA, the NSS scores were notably decreased on day 3, 7 and 12 in the EA group compared with the model group(P<0.05, P<0.01). After modeling, the expression levels of Wnt7 a and LEF1 mRNAs from 3 h to 12 d, Wnt7 a and LEF1 proteins from 6 h to 12 d were considerably increased(P<0.01, P<0.05), while those of GSK-3β mRNA at 9, 12 and 24 h, GSK-3β protein at 24 h and 3 d, and DKK1 mRNA at 24 h and 3 d and DKK1 protein at 3 d were obviously decreased in the model group relevant to the sham-operation group(P<0.05, P<0.01). After the intervention, the expression levels of Wnt7 a mRNA at 12 h to 3 d, Wnt7α protein from 24 h to 12 d, LEF1 mRNA from 24 h to 12 d, and LEF1 protein from 3 d to 12 d were further apparently up-regulated(P<0.01, P<0.05), while those of GSK-3β mRNA at 9 h, 3,7 and 12 d, and GSK-3β protein at 12 h, 7 d and 12 d, and DKK1 mRNA at 12 h, 24 h and 3 d, and DKK1 protein at 24 h to 12 d were obviously down-regulated in the EA group relevant to the model group(P<0.01, P<0.05). No significant difference was found between the blank and sham-operation groups in the NSS scores and expression levels of Wnt7 a, LEF1, GSK-3β and DKK1 mRNAs and proteins at all the time points(P>0.05). Conclusion EA of GV26 can significantly improve the neurological deficit symptoms in MCAO rats, which may be associated with its effects in up-regulating the expression of Wnt7 a and LEF1 mRNAs and proteins, and in down-regulating the expression of GSK-3β and DKK1 mRNAs and proteins.关键词：Cerebral infarction;Electroacupuncture;Shuigou(GV26);Neurological function;Wnt signaling pathway274|394|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture(Acupunct) on cerebral infarction volume and expression of poly ADP ribose polymerase 1(PARP1), apoptosis-inducing factor(AIF) and endonuclease G(Endo-G) in the cerebral cortex tissue at different time-points after cerebral ischemia(CI) in acute cerebral infarction rats, so as to explore its underlying mechanisms in prolonging time window of thrombolysis. Methods Forty-eight SD rats were randomly divided into sham operation, model, intravenous thrombolysis(IVT)-4.5 h, IVT-6 h, IVT-9 h, Acupunct+IVT-4.5 h, Acupunct +IVT-6 h, Acupunct+IVT-9 h groups(n=6 in each group). The CI model was established by using modified autologous thromboembolism via the right common carotid artery. Two hours after modeling, rats of the Acupunct groups received Acupunct stimulation of "Shuigou"(GV26) and bilateral "Neiguan"(PC6) for 30 min. Thrombolysis was conducted by injection of recombinant human tissue-type plasminogen activator(rt-PA, 10 mg/kg) via caudal vein. The neurological deficit was assessed with reference to Bederson's methods. 2,3,5-triphenyltetrazolium chloride(TTC) staining was used to assess the cerebral infarction volume, and the expression of cerebral PARP1, AIF and Endo-G proteins detected by Western blot. Results Compared with the sham operation group, the neurological score and percentage of cerebral infarction volume, expression levels of PARP1, AIF and Endo-G proteins were significantly increased in the model group(P<0.01,P<0.05). After the intervention, modeling-induced increase of the aforementioned indexes was reversed in the IVT-4.5 h, Acupunct+IVT-4.5 h and Acupunct+IVT-6 h groups(P<0.05), except PARP1 expression of the Acupunct+IVT 6 h group(P>0.05). The levels of neurological score, percentage of cerebral infarction volume, and AIF expression were significantly lower in both the Acupunct+IVT 4.5 h and Acupunct+IVT-6 h groups than in the simple IVT-4.5 h and simple IVT-6 h groups, respectively(P<0.05), and the expression levels of PARP1 and Endo-G proteins were obviously lower in the Acupunct+IVT-4.5 h group than in the IVT-4.5 h group(P<0.05). Endo-G proteins were obviously lower in the Acupunct+IVT-9 h group than in the IVT-9 h group(P<0.05). Conclusion Acupuncture may improve neurological function, reduce cerebral infarction volume and prolong the time window of thrombolysis in CI rats, which may be associated with its effect in suppressing AIF/PARP1/Endo-G signaling.关键词：Thrombolysis time window;Cerebral infarction;Acupuncture;Poly ADP ribose polymerase 1;Apoptosis-inducing factor;Endonuclease G341|637|10更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion at "Zusanli"(ST36) on peripheral and central IL-6, IL-10 and IL-10/IL-6 changes in fatigue rats, so as to explore its mechanisms underlying relief of fatigue. Methods Twenty-one male SD rats were randomly divided into normal control, model and moxibustion groups with 7 rats in each group. The fatigue model was induced by exhausted weight-loaded swimming, once daily for 21 days. Moxibustion was applied to bilateral ST36 once every other day, for successive 11 times. The overall state and the body weight were observed. The levels of IL-6 and IL-10 in the serum, gastrocnemius and frontal cortex tissues were detected by enzyme-linked immunosorbent assay(ELISA). Results Compared with the normal group, the body weight was significantly reduced on the 7(th), 14(th), 14(th), and 21(th), and 21(st) day in the model group(P<0.05), and considerably increased on the 14(st) day in the model group(P<0.05), and considerably increased on the 14(th) and 21(th) and 21(st) day in the moxibustion group relevant to the model group(P<0.05). After modeling, the levels of IL-6 in the serum, gastrocnemius and frontal cortex were significantly increased(P<0.05, P<0.01), while those of IL-10 was obviously decreased(P<0.05, P<0.01) in the model group relevant to the normal group, and ratio of IL-10/IL-6 was obviously decreased in the serum and frontal cortex of the model group relevant to the normal group(P<0.01). After the moxibustion intervention, the levels of IL-10 in the serum and frontal cortex were significantly increased(P<0.05), and ratio of IL-10/IL-6 in the serum and frontal cortex was significantly increased(P<0.01), while the IL-6 contents in the gastrocnemius and frontal cortex were notably decreased in the moxibustion group relevant to the model group(P<0.05). Conclusion Moxibustion can inhibit weight-loaded swimming induced decrease of body weight in fatigue rats, which may be associated with its effects in balancing the levels of peripheral and central pro-inflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10.关键词：Moxibustion;Fatigue;Body weight;Serum;Gastrocnemius;Frontal cortex;IL-6;IL-10284|313|0更新时间：2023-08-11
- 摘要：Objective To observe the effect of fire-needle stimulation of "Neixiyan"(EX-LE4) and "Dubi"(ST35) on changes of motor function, structure of cartilage degradation and inflammatory factors in knee osteoarthritis(KOA) rats, so as to explore its underlying mechanisms in improving KOA. Methods Thirty-nine male SD rats were randomly divided into normal, model and fire-needle groups(n=13 in each group). The KOA model was established by injection of Monoiodoacetate(MIA, 1 mg) into the lumen of the right knee joint. On the 7(th) day after successful modeling, fire-needle was applied to EX-LE4 and ST35, twice a week for 3 weeks. The rats' behavioral reactions of gait(0 to 3 points) and claw pressure(0 to 3 points) were scored, and histopathological changes scored by assessing the impairment grade(0 to 6) and stage(0 to 4) of the articular cartilage after safranin O-fast green staining. The contents of serum Interleukin(IL)-1α, Tumor necrosis factor α(TNF-α), IL-10, IL-37 and transforming growth factor β(TGF-β) were assayed by ELISA, and ultrastructural changes were observed under transmission electron microscope(TEM). Results After modeling, the gait and claw-pressure scores were significantly increased in the model group in comparison with the normal group(P<0.05), and the OA score and contents of serum IL-1α and TNF-α were also appa-rently increased in the model group relevant to the normal group(P<0.05,P<0.01), while the levels of serum IL-10, IL-37 and TGF-β remarkably decreased in the model group in contrast to the normal group(P<0.01). After the intervention, the increase of gait and claw-pressure and OA scores, as well as serum IL-1α and TNF-α contents, and the decrease of serum IL-10, IL-37 and TGF-β levels were all reversed in the fire needle group(P<0.05, P<0.01). Outcomes of TEM showed more and larger lipid droplets, swollen mitochondria with some vacuoles, and expanded, broken or dissolved rough endoplasmic reticulum in the model group, which was milder in the fire-needle group. Conclusion Fire-needle can improve motor function and relieve impairment of articular cartilage of KOA rats, which may be related to its effects in reducing inflammatory factors and in increasing anti-inflammatory factor levels.关键词：Fire-needle;Knee osteoarthritis;Interleukin;Tumor necrosis factor α;Transforming growth factor β253|667|5更新时间：2023-08-11
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Display of low hydraulic resistance channels along Conception Vessel by in vivo fluorography in rats
摘要：Objective To display the low hydraulic resistance channel along the running course of the Conception Vessel(CV) by using in vivo fluorography, so as to provide evidence for the existence of meridians of traditional Chinese medicine from the aspect of interstitial fluid channel. Methods A total of 24 male SD rats were randomly divided into 3 groups, namely CV-low impedance spots(CV-LIS), CV-low hydraulic resistance points(CV-LHRP) and non-meridian points(NMP) groups(n=8 in each group). The CV-LIS(the imaged line between the xiphoid process and symphysis pubis), CV-LHRP(lateral and parallel to LIS) and NMP(lateral and parallel to CV-LHRP) were detected by using meridian detector and biofluid resistance meter, respectively. Sodium fluorescein solution(1%) was injected into the spot(A-spot, upper 1/5 of the CV-LIS), and one of the NMP(about 1 cm lateral and parallel to the A-spot, and the mid-point between the Kidney and Stomach meridian running courses) by using a microsyringe for observing the migration trace of sodium fluorescein. Results After injection of fluorescein, the migration trace of fluorescein appeared along the CV-LIS and CV-LHRP lines, and only local diffusion was found in the NMP group. The migration trace lengths along the CV-LIS and CV-LHRP were significantly longer than that of the NMP group(P<0.01), and the migration width of CV-LIS and CV-LHRP groups was significantly smaller than that of the NMP group(P<0.01). The migration speed of CV-LIS and CV-LHRP groups was significantly higher than that of the NMP group(P<0.01). Conclusion Fluorography is able to be used to display the running course of CV, possibly supporting the existence of meridians from the interstitial fluid channel and providing a new method for visualization of meridians.关键词：Meridians;Low hydraulic resistance channel;Sodium fluorescein;Fluorography;Conception Vessel259|342|6更新时间：2023-08-11 - 摘要：Objective To observe the influence of Xiao's "xingnaofusu" needling(the technique for resuscitation) on regaining consciousness in the patients with persistent vegetative state(PVS). Methods A total of 50 patients of PVS were randomized into an observation group and a control group, 25 cases in each. The patients in the control group were treated by the routine western medicine, and those in the observation group treated by Xiao's "xingnaofusu" needling and routine western medicine. Baihui(GV20), Dingshen(Extra) to Shangen(Extra)(penetrating technique), Fengchi(GB20) to GB20(penetrating technique), Neiguan(PC6) to Waiguan(TE5)(penetrating technique), Hegu(LI4) to Laogong(HT8)(penetrating technique) and Taichong(LR3) to Yongquan(KI1)(penetrating technique) were selected. The treatment was given once a day, 10 days as one treatment course, 3 courses in total. The coma recovery scale-revised(CRS-R) score, the modified Ashworth scale(MAS) score and the Glasgow coma scale(GCS) were separately compared before and after the treatment. Additionally, CT scanning was adopted to measure the width of the third ventricle before and after treatment so as to evaluate the clinical therapeutic effect. Results After the treatment, the CRS-R and GCS scores in the two groups increased remarkably, and MAS score reduced obviously as compared with that before the treatment(P<0.05); and the CRS-R and GCS scores were higher, and MAS score lower in the observation group than those in the control group(P<0.05). Compared with the control group, the width of the third ventricle reduced obviously in the observation group after the treatment(P<0.05). At the end of the treatment courses, the effective rate was 79.2%(19/24)in the observation group and was 47.8%(11/23) in the control group. The effective rate of the observation group was obviously higher than that of the control group(P<0.05). Conclusion Xiao's "xingnaofusu" needling can remarkably improve the central nerve function, promote the recovery of brain function and the motor function of limbs, reduce the width of the third ventricle and improve the clinical therapeutic effect of regaining consciousness in the patients with PVS.关键词：Xiao's "xingnaofusu" needling;Persistent vegetative state;Therapeutic effect of regaining consciousness;The third ventricle237|355|0更新时间：2023-08-11
- 摘要：Objective To investigate the disease spectrum and predominant diseases treated by abdominal acupuncture by data mining and analysis of journal articles on abdominal acupuncture, and to provide a reference for clinical diagnosis and treatment. Methods Based on the database of abdominal acupuncture established by the research group, the data mining technique was used for the analysis and extraction of the articles on abdominal acupuncture included in this study. Results A total of 788 original journal articles were included. Six departments and 96 disease categories, among which there were 45 internal diseases(46.84%) and 18 surgical diseases(18.75%) were involved. As for the diseases involved, cervical spondylosis had the highest frequency of 84, followed by low back and leg pain with a frequency of 77 and stroke sequela with a frequency of 67. Of all 788 studies, 519(65.86%) used abdominal acupuncture combined with other therapies with a total frequency of 552, among which acupuncture had the highest frequency of 135(24.46%), followed by oral administration of traditional Chinese medicine with a frequency of 81(14.67%) and moxibustion with a frequency of 80(14.49%). Abdominal acupuncture had a marked clinical effect in the treatment of various diseases, with the highest effective rate of 95.10% in surgical diseases. Conclusion Abdominal acupuncture has a wide disease spectrum and is most frequently used for the treatment of cervical spondylosis, with a marked clinical effect. Abdominal acupuncture has unique therapeutic characteristics and advantages, but it can achieve a better clinical effect when combined with other therapies.关键词：Abdominal acupuncture;Data mining;Disease spectrum;Therapeutic characteristics299|923|9更新时间：2023-08-11
- 摘要：Objective To evaluate the clinical effect of acupuncture combined with Chinese medicine in the treatment of oligozoospermia/asthenozoospermia. Methods Randomized controlled trials(RCTs) of acupuncture combined with traditional Chinese medicine(TCM) in the treatment of oligozoospermia/asthenozoospermia published up to February 15, 2019 were searched from databases of CNKI, CBM, Wanfang Data, VIP, PubMed, Embase, and The Cochrane Library. Two reviewers independently performed quality assessment and data extraction of the studies, and RevMan5.3 was used to perform the meta-analysis. Results A total of 13 articles with 12 RCTs were included, with a total of 1 183 patients. The meta-analysis showed that acupuncture combined with TCM achieved a significantly higher pregnancy rate than TCM alone in the treatment of oligozoospermia/asthenozoospermia(risk ratio [RR] =1.46, 95% confidence interval [CI]: 1.13-1.90,P=0.004). The patients treated with acupuncture combined with TCM had a significantly higher effective rate than those treated with TCM alone(RR=1.17, 95% CI:1.10-1.24, P<0.000 01). Compared with TCM alone, acupuncture combined with TCM achieved significantly better improvements in sperm survival rate(mean difference [MD]=8.28, 95% CI: 6.48-10.08, P<0.000 01), sperm motility(MD=17.01, 95%CI:11.06-22.96, P<0.000 01), sperm concentration(MD=8.71, 95%CI:5.92-11.50, P<0.000 01), and number of grade A sperms(MD=6.39, 95%CI:5.27-7.50, P<0.000 01). Conclusion Acupuncture combined with TCM has a better clinical effect than TCM alone in the treatment of oligozoospermia/asthenozoospermia. Due to the low methodological quality of the studies included in this analysis, acupuncture combined with TCM in the treatment of oligozoospermia/asthenozoospermia should be used based on patients' conditions in clinical practice.关键词：Acupuncture combined with traditional Chinese medicine;Oligozoospermia;Asthenozoospermia;Meta-analysis247|1149|6更新时间：2023-08-11
- 摘要：Acupuncture has become an effective approach in clinic for treating obesity, but its mechanism has not been clarified yet. A large number of researches have been conducted on the obesity mechanism in the aspects of neurophysiological regulation, feeding center regulation and peripheral digestion and absorption regulation at home and abroad. But, regarding the main storage site of excess energy, i.e. the remodeling and functional regulation of white adipose tissue(WAT), is still a new field in research. In the paper, focusing on the new filed of weight loss, in view of the promotion of WAT browning through the re-gulation of UCP1 and PPARγ signal pathway with acupuncture, the potential peripheral mechanism of acupuncture was explored on weight loss.关键词：Acupuncture-moxibustion;Peroxisome proliferators activated receptor γ;Un-coupling protein 1;White adipose tissue browning258|975|4更新时间：2023-08-11
- 摘要：The literature of experimental research on diet-induced obesity treated with acupuncture was retrieved. The aspects of epigenetics, neuroendocrine system, intestinal flora, inflammatory response, oxidative stress and substance metabolism of the mechanism of acupuncture in the treatment of diet-induced obesity were summarized. It is suggested that the potential mechanism of acupuncture in the treatment of diet-induced obesity should be discussed in the aspects of the interaction of Toll-like receptors on obesity-intestinal flora-immune function, the improvement of insulin resistance, epigenetics and antioxidant stress.关键词：Acupuncture;Diet-induced obesity;Mechanism;Review272|923|2更新时间：2023-08-11
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