最新刊期
卷 45 ， 期 8 ， 2020
- 摘要：Objective To investigate the effect of electroacupuncture(EA) on the p38 mitogen-activated protein kinase(p38 MAPK) and signal transducer and activator of transcription 3(STAT3) pathway in hippocampus and frontal cortex of diabetic rats with cognitive impairment(CI), as well as the mechanism of EA in protection against CI in diabetic rats. Methods Thirty SD rats were divided into normal, model and EA groups(n=10 rats/group). The diabetic model was established by i.p.injection of Streptozotocin solution(25 mg/kg), followed by high-fat diet raising for 1 month, and the CI rats was confirmed by Morris water maze tasks. The rats in the EA group were given acupuncture at "Zusanli"(ST36) "Neiting"(ST44) and "Yishu"(EX-B3) 20 min/d, among which ST36 and ST44 were treated with EA. The treatment was conducted 6 times a week for 4 weeks. The fasting blood glucose(FBG) contents were assayed by glucometer before and after treatment. The rats' learning-memory ability was detected by Morris water maze tasks. The expression levels of IL-6、IL-1β、TNF-α、p38 MAPK、p-p38 MAPK、STAT3 and p-STAT3 in hippocampus and frontal cortex were detected by Western blot and quantitative real-time PCR, separately. The mean fluorescence intensity of p38 MAPK and STAT3 was observed by immunofluorescence histochemistry. Results After modeling, FBG and the escape latency of Morris water maze tasks were significantly increased in the model group compared with the normal group(P<0.001, P<0.01). Following EA treatment, the increased FBG and average escape latency were markedly reversed in the EA group relevant to the model group(P<0.05). Compared with the normal group, the proteins and mRNAs expression of IL-6, IL-1β, TNF-α, p38 MAPK, p-p38 MAPK, STAT3 and p-STAT3 in hippocampus and frontal cortex were significantly increased in the model group(P<0.001), as well as the mean fluorescence intensity of p38 MAPK and STAT3 in hippocampus and frontal cortex(P<0.001). Following EA intervention, the proteins and mRNAs expression of IL-6, IL-1β, TNF-α, p38 MAPK, p-p38 MAPK, STAT3 and p-STAT3, and the mean fluorescence intensity of p38 MAPK and STAT3 in hippocampus and frontal cortex were down-regulated(P<0.001, P<0.05). Conclusion EA can inhibit the over production of pro-inflammatory cytokines in diabetic rats with CI, possibly by regulating the expression of p38 MAPK and STAT3 pathway.关键词：Diabetic rats with cognitive impairment;Electroacupuncture;Inflammatory factor;p38 mitogen-activated protein kinase;STAT3393|904|5更新时间：2023-08-11
- 摘要：Objective To investigate the effect of electroacupuncture(EA) at "Baihui"(GV20), "Fengfu"(GV16) and bilateral "Shenshu"(BL23) on learning-memory ability, apoptosis in the hippocampus and expression of Aβ, Caspase 3, Bax and Bcl-2 proteins in the hippocampus and cerebral cortex in immature mice with Alzheimer's disease(AD), so as to explore its mechanism underlying improvement of AD. Methods Forty APP/PS1 transgenic male young mice were equally randomized into model and EA groups and 20 C57 BL/6 J male young mice were used as the normal control. EA(10 Hz, about 2 mA) was applied to GV20-BL23 and GV16-BL23 for 20 min, once daily, 6 days a week for 16 weeks. The Morris water maze swimming test was used to evaluate the animals' learning-memory ability. Congo red staining and immunohistochemical staining were used to detect senile plaques in the hippocampus(dentate gyrus) and cerebral cortex tissues. Terminal deoxynucleotidyl transferase-mediated dUTP Nick-end Labeling(TUNEL) was used to detect the cellular apoptosis of hippocampus. The expression levels of apoptosis related factors Caspase 3, Bax and Bcl-2 were detected by Western blot. Results After modeling, the escape latency of place navigation test of Morris water maze swimming tasks was significantly increased(P<0.05), while the number of platform crossing and residence time in the platform quadrant of spatial exploration test were significantly decreased in the model group in contrast to the normal control group(P<0.05). The number of apoptotic cells in the hippocampus and expression levels of Aβ, Caspase 3 and Bax proteins in the hippocampus and cerebral cortex were significantly up-regulated in the model group relevant to the normal control group(P<0.05). Following EA intervention, the escape latency of place navigation test of Morris water maze swimming tasks was significantly decreased(P<0.05), while the number of platform crossing and residence time in the platform quadrant of spatial exploration test were significantly increased in the EA group in contrast to the model group(P<0.05). The hippocampal apoptotic cells, the expression of Aβ, Caspase 3 and Bax proteins in hippocampus and cerebral cortex were evidently down-regulated in the EA group in contrast to the model group(P<0.05). Whereas the ratio of Bcl-2/Bax was significantly decreased in the model group relevant to the normal control group(P<0.05) and considerably increased in the EA group in contrast to the model group(P<0.05). No significant changes were found in the expression levels of Bcl-2 after modeling and after EA intervention(P>0.05). Conclusion EA of GV20, GV16 and BL23 can effectively improve the learning-memory ability in AD mice, which may be related to its function in inhibiting neuronal apoptosis in the hippocampus and down-regulating the expression levels of Aβ, Caspase 3 and Bax proteins in both hippocampus and cerebral cortex.关键词：Electroacupuncture;Alzheimer's disease;Learning-memory ability;Apoptosis;β-amyloid;Hippocampus;Cerebral cortex342|611|8更新时间：2023-08-11
- 摘要：Objective To compare the effect of electroacupuncture(EA) of acupoint group for "reinforcing the kidney and regulating Governor Vessel" and acopoint group for "reinforcing the kidney and lung and regulating Governor Vessel" on lear-ning-memory ability and expression of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) proteins in the hippocampus and prefrontal cortex(PFC) in Alzheimer's disease(AD) rats, so as to explore the efficacy of the two acupoint groups and mechanisms underlying improvement of AD. Methods Forty male SD rats were randomly divided into control, sham operation, model, "Baihui" + "Shenshu"(GV20+BL23, for "reinforcing the kidney and regulating Governor Vessel") EA and GV20+BL23+ "Feishu"(BL13, GV20+BL23+BL13, for "reinforcing the kidney and lung and regulating Governor Vessel") EA groups(n=8 rats in each group). The AD model was established by bilateral injection of amyloid β peptide(Aβ_(25-35),10 μL) into bilateral hippocampus, and rats of the sham operation group received injection of normal saline. After successful establishment of the model,EA(2 Hz, 2 mA) was applied to these acupoints for 15 min, once daily for 10 days. Then, the learning-memory ability was assessed by using Morris water maze tests, and the expression levels of TNF-α and IL-1β proteins in the PFC and hippocampus tissues were detected by using Western blot. Results Following modeling, the average escape latency of place navigation test were significantly increased(P<0.05) and the platform crossing times of spatial probe test was significantly decreased in the model group than in the control and sham operation groups(P<0.05). The expression levels of IL-1β and TNF-α proteins in the PFC and hippocampus were apparently up-regulated in the model group than in the control and the sham operation groups(P<0.000 1, P<0.001, P<0.01). After the intervention, the increase of the average escape latency and expression of IL-1β and TNF-α in the PFC and hippocampus, and the decrease of space exploration test were revised in both GV20+BL23 EA and GV20+BL23+BL13 EA groups(P<0.05,P<0.01). No significant differences were found between the GV20+BL23 and GV20+BL23+BL13 EA groups in the above mentioned indexes(P>0.05). Conclusion EA of both GV20+BL23 and GV20+BL23+BL13 acupoint can improve learning-memory ability of AD rats, which is associated with their effects in down-regulating the expression of IL-1β and TNF-α in the PFC and hippocampus to reduce inflammatory reaction. There were no significant differences between the two acupoint groups in the therapeutic effects.关键词：Electroacupuncture;Alzheimer's disease;Prefrontal cortex;Hippocampus;Learning-memory ability;Interleukin-1β;Tumor necrosis factor-α349|503|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of wrist-ankle acupuncture(WA) stimulation at "R_4"-"R_5"-"R_6" on the expression of glutamate(Glu) and phosphorylated protein NMDAR1(p-NMDAR1) of the spinal dorsal horn in spared nerve injury(SNI) rats, so as to explore its mechanism underlying improvement of SNI. Methods A total of 36 SD rats were randomly divi-ded into sham operation, model and WA groups, with 12 rats in each group. The SNI procedure comprised an axotomy and ligation of the tibial and common peroneal nerves leaving the sural nerve intact. Rats of the WA group were treated by acupuncture at "R_4"-"R_5"-"R_6" points from the 5(th) day to the 14(th) day to the 14(th) day after modeling. The mechanical pain thresholds were measured before and 5, 10 and 14 d after SNI, respectively. The cold allodynia was dectected by Acetone solution dropped onto the lateral plantar surface of the paw. Glu content and p-NMDAR1 expression of spinal dorsal horn were detected by (th) day after modeling. The mechanical pain thresholds were measured before and 5, 10 and 14 d after SNI, respectively. The cold allodynia was dectected by Acetone solution dropped onto the lateral plantar surface of the paw. Glu content and p-NMDAR1 expression of spinal dorsal horn were detected by 1H-MRS, ELISA and immunohistochemistry methods. Results Compared with the sham operation group, the mechanical pain threshold of the model group was significantly decreased(P<0.01), the duration of cold stimulation foot contraction was increased(P<0.01), and the Glu content and p-NMDAR1 expression in the spinal dorsal horn were significantly increased(P<0.05, P<0.01). After WA intervention, the mechanical pain threshold was significantly increased(P<0.01), the duration of cold stimulation was significantly shortened(P<0.01), and Glu content and p-NMDAR1 protein expression of spinal dorsal horn were decreased significantly(P<0.05, P<0.01) in the WA group compared with the model group. Conclusion WA can reduce pain sensitivity in rats with neuropathic pain, possibly by inhibiting the expression of Glu and p-NMDAR1 in the spinal dorsal horn.关键词：Neuropathic pain;Wrist-ankle acupuncture;Spinal dorsal horn;Glutamate;NMDAR1325|497|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on liver surface blood perfusion and related indexes of oxidative stress in mice with acute alcoholic liver injury(AALI), so as to explore its mechanism underlying improving of AALI. Methods Twenty-four Kunming mice were randomly divided into control, model and EA groups(8 mice/group). AALI model was established by intragastric administration of 50% ethanol at 6 g/kg. EA(4-6 V, 50 Hz) was applied to bilateral"Zusanli"(ST36) and"Taichong"(LR3) for 30 min. The liver surface blood perfusion was dynamically observed by moorFLPI laser speckle imager for 30 min. The morphological changes of the liver tissue were determined by H.E. staining. Levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in the liver were measured by ELISA. Results Compared with the control group, the model group had markedly pathological changes of the hepatic cords, hepatic sinuses and hepatocytes shown by H.E. staining, a significant reduction in liver surface blood perfusion and the content of SOD in liver tissue(P<0.01, P<0.05), and a significant increase in the content of MDA in liver tissue(P<0.05). Compared with the model group, H.E. staining showed reduced pathological changes of the liver tissue, meanwhile the liver surface blood perfusion significant increased(P<0.05) and the content of MDA in liver tissue significantly decreased(P<0.01) in the EA group. Conclusion EA can improve the liver surface circulation of AALI mice, and regulate the oxidative stress response, and thus help to repair the damage of liver tissue.关键词：Acute alcoholic liver injury;Electroacupuncture;Liver surface blood perfusion;Superoxide dismutase;Malondialdehyde294|325|0更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion on serum inflammatory factors, Toll-like receptor 4(TLR4), myeloid differentiation factor 88(MyD88) and nuclear factor Kappa B(NF-κB) in colon tissue of rats with diarrhea-predominant irritable bowel syndrome(IBS-D),so as to explore the mechanism of moxibustion in the treatment of IBS-D. Methods Healthy male SD rats were randomly divided into blank group, model group and moxibustion group, with 8 rats in each group. The IBS-D model was established by chronic restraint combined with gavage of Senna-leaf solution. The rats of the moxibustion group was treated with moxibustion at "Tianshu"(ST25)and "Shangjuxu"(ST37)for 30 min, once a day for 7 d. After the intervention, the rate of loose stools and the minimum threshold volume of abdominal withdrawal reflex(AWR) induced by colorectal distension were observed. The contents of serum inflammatory factors IL-1β, TNF-α and IL-6 were detected by ELISA. The pathological changes of rat colon were observed by H.E. staining. The average optical density of TLR4 and NF-κB(p65) in colon tissue was detected by immunohistochemistry. The relative expressions of TLR4, MyD88 and NF-κB(p65) mRNAs in colon tissues were detected by real-time fluorescence quantitative PCR, and the expression levels of TLR4, MyD88 and NF-κB(p65) proteins in colon tissues were detected by Western blot. Results There was a mild degree of inflammation in colon in rats of the model group. Compared with the blank group, the minimum volume threshold of AWR decreased significantly(P< 0.01), and the rate of loose stools, the contents of IL-1β, IL-6 and TNF-α in serum as well as the expressions of TLR4, MyD88, NF-κB(p65) mRNAs and protein in colon tissues were significantly increased in the model group(P<0.01). Compared with the model group, the inflammation was alleviated in colonic mucosa, the minimum volume threshold of AWR increased significantly(P<0.01), and rate of loose stools decreased significantly(P<0.01) in the moxibustion group, the contents of IL-1β, TNF-α and IL-6 in serum and the expression levels of TLR4, MyD88, NF-κB(p65) mRNAs and proteins in colon tissue decreased significantly in the moxibustion group(P<0.01). Conclusion Moxibustion ST25 and ST37 can improve diarrhea symptom and visceral hypersensitivity in IBS-D rats, which may be related with its effects in inhibiting TLR4/MyD88/NF-κB signaling pathway and reducing the expression of inflammatory factors.关键词：Diarrhea-predominant irritable bowel syndrome;Moxibustion;Inflammatory cytokine;TLR4/MyD88/NF-κB signaling pathway343|1280|9更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the expression of autophagy mediated PI3 K/AKT pathway and sex hormones in polycystic ovary syndrome(PCOS) rats, so as to explore the mechanisms underlying improvement of PCOS anovulatory infertility. Methods Six-week-old female Sprague-Dawley rats were randomly divided into control, model and EA groups(n=10 per group). The PCOS model was established by gavage of Letrozole solution(1.0 mg/kg), once daily for 21 consecutive days. Then, EA(2 Hz, 1 mA) was applied to the "Sanyinjiao"(SP6) and "Taichong"(LR3) for 20 min, once a day for 14 successive days. The morphological changes of the ovary were observed after H.E. staining. The contents of serum androgen(T), luteinizing hormone(LH) and anti-mullerian hormone(AMH) were detected by ELISA. The expression levels of phosphatidylinositol 3-kinase(PI3 K), protein kinase B(AKT) and microtubule-associated protein 1 light chain 3(LC3) in the ovary tissues were detected by Western blot. Results Compared with the control group, the levels of serum T, LH and AMH contents, and ovarian LC3 Ⅱ expression and the ratio of LC3 Ⅱ/Ⅰwere significantly increased(P<0.05), and the expression levels of PI3 K and AKT proteins were considerably decreased in the model group(P<0.05). After EA intervention, the levels of serum T, LH, AMH, and the ratio of LC3 Ⅱ/Ⅰwere considerably down-regulated(P<0.05), and those of PI3 K and AKT were obviously up-regulated in the EA group(P<0.05). Conclusion EA intervention can reduce serum T, LH and AMH contents, and the ratio of ovarian LC3 Ⅱ/Ⅰ and up-regulate ovarian PI3 K and AKT in PCOS rats, which may contribute to its effect in improving anovulatory infertility by suppressing autophagy.关键词：Electroacupuncture;Polycystic ovary syndrome;Ovulation disorders;Autophagy336|560|7更新时间：2023-08-11
- 摘要：Objective To compare the analgesic effect of manual acupuncture(MA) stimulation of "Zusanli"(ST36) in rats with inflammatory pain under unrestrained conscious, restrained and general anesthesia conditions, so as to explore the applicability of unrestrained conscious model in the evaluation of acupuncture analgesia effect. Methods Male SD rats were divided into 5 groups: blank control(n=9), pain model(n=7), unrestrained conscious conditions+MA(n=6), restrained conditions+MA(n=6), and general anesthesia(GA)+MA(n=6). The acute pain model was established by injection of complete Freund's adjuvant(CFA) into the left ankle joint 48 h ahead of acupuncture. Subsequently, a single 20 min session of MA was applied to the left ST36. The mechanical and thermal pain thresholds(MPT and TPT) were determined before and after injection of CFA, and after MA stimulation. In order to evaluate the autonomic behavior activities, rats were randomly divided into blank control(n=11), pain model(n=11) and conscious-unrestrained conditions +MA(n=12) groups. The rats' exploratory movements were assessed by open field tests. Results Both MPT and TPT were significantly decreased after injection of CFA in the model group relevant to the blank control group(P<0.001), and significantly higher in the three MA groups than in the model group(P<0.001). Comparison among the three MA groups showed that both MPT and TPT were significantly higher in the conscious unrestrained conditions+MA group than in the restrained conditions+MA and GA+MA groups(P<0.05, P<0.01). Open filed tests showed that the total moving distance in the open field and wall climbing times were significantly lower in the model group than in the blank control group(P<0.01), and the wall climbing times were obviously more in the unstrained conditions+MA group than in the model group(P<0.05). The central area resistance time was significantly shorter in the model group than in the control group(P<0.05), and was moderately increased after MA despite no evident significance(P>0.05). No significant changes were found in the total moving distance after MA and in the central area moving distance after modeling and MA(P>0.05). Conclusion MA has a better therapeutic effect in relieving pain and pain-induced depression-like behavior in conscious unrestrained rats than in restrained and GA rats, implying a higher applicability of unrestrained conscious pain model to the assessment of acupuncture analgesia.关键词：Unrestrained conscious model;Acupuncture analgesia;Pain threshold;Autonomic behavior276|438|2更新时间：2023-08-11
- 摘要：Objective To observe the short-term and long-term clinical effect on posterior circulation ischemic vertigo treated with "xiao xingnao kaiqiao" acupuncture(minor regaining consciousness and opening orifice) and explore its effect mechanism. Methods Ninety patients with posterior circulation ischemic vertigo were randomly divided into a treatment group and a control group, 45 cases in each group. The patients of the two groups were all treated on the base of neurological medicine. In the control group, Flunarizine Hydrochloride was prescribed for oral administration(5 mg, once daily, for 21 days totally). In the treatment group, acupuncture of "xiao xingnao kaiqiao" was provided at Yintang(EX-HN3), bilateral Neiguan(PC6), bilateral Sanyinjiao(SP6), Baihui(GV20), bilateral Fengchi(GB20), bilateral Wangu(GB12) and bilateral Tianzhu(BL10). The needles were retained for 30 min, once daily for 21 days totally. The changes in vertigo score of traditional Chinese medicine(TCM) were observed, and the changes in the mean blood velocity(Vm) of the left vertebral artery(LVA), the right vertebral artery(RVA) and the basilar artery(BA) as well as the vascular pulsatility index(PI) were monitored and determined by transcranial Doppler(TCD). Additionally, the recurrence rate was followed up after 3 months to evaluate the long-term clinical effects. Results After treatment, the total effective rate of the treatment group was 91.11%(41/45) and 75.56%(34/45) in the control group. The total effective rate in the treatment group was higher than that in the control group(P<0.05). Compared with their own pre-treatment, the vertigo scores of TCM were reduced in either the treatment group or the control group after treatment(P<0.05) and the score in the treatment group was lower than that in the control group(P<0.05). Compared with their own pre-treatment, Vm and PI were all improved after treatment in either group(P < 0.05). After treatment, the improvements in Vm and PI of LVA、RVA and BA in the treatment group were better than those in the control group(P<0.05). In the follow-up after 3 months, the recurrence rate was 19.51%(8/41) in the treatment group and was 50.00%(17/34) in the control group. The recurrence rate in the treatment group was lower than that in the control group(P<0.05). Conclusion "Xiao xingnao kaiqiao" acupuncture obviously relieves the clinical symptoms of posterior circulation ischemic vertigo. The mechanism of acupuncture is potentially related with its effects in improving Vm and PI of LVA,RVA and BA, as well as improving blood supply of brain tissue.关键词：Xiao xingnao kaiqiao acupuncture;Vertigo;Posterior circulation ischemia298|899|3更新时间：2023-08-11
- 摘要：Objective To compare the differences in the clinical effect on post-stroke shoulder hand syndrome(SHS) stage Ⅰ between the along-meridian trochar acupuncture therapy and the routine acupuncture therapy with filiform needles. Me-thods A total of 80 patients with post-stroke SHS stage I were divided into a treatment group(41 cases) and a control group(39 cases) according to the random number table. In the control group, the common filiform needles were used to stimulate Jianyu(LI15), Jianliao(TE14), Jianzhen(SI9), Jianzhongshu(SI15), Jianwaishu(SI14), 5 times a week, 3 weeks as 1 course. In the treatment group, along-meridian trochar acupuncture therapy was applied, 3 times a week, 3 weeks as 1 course. The patients in both groups were all treated with basic medications and routine rehabilitation training. Pain degree, edema degree, upper limb motor function and activity of daily living were observed in the two groups before the treatment, at the end of the treatment and in follow-up. At the end of treatment and in follow-up, the therapeutic effect was evaluated respectively in the patients of the two groups. Results Compared with the values before treatment, the VAS score of the upper limb was reduced obviously(P< 0.001), the score of the upper limb motor function and Barthel index were increased obviously(P<0.001, P<0.05) in the patients of the two groups, the score of edema degree of the affected limb was reduced after treatment in the patients of the treatment group(P<0.001). Compared with the control group, VAS score of the upper limb and the score of edema degree of the affected limb were obviously lower(P<0.001), and the score of the upper limb motor function and Barthel index were obviously higher in the treatment group(P<0.001). The total effective rate was 66.7%(26/39) after treatment and was 74.4%(29/39) in follow-up in the treatment group and they were 20.5%(8/39) and 28.2%(11/39) respectively in the control group. The total effective rates after treatment and in follow-up in the treatment group were all obviously higher than those in the control group respectively(P<0.001). Conclusion The along-meridian trochar acupuncture therapy remarkably relieves pain and edema and improves the upper limb motor function and the activity of daily living in the patients with post-stroke shoulder hand syndrome and its clinical therapeutic effect is definite.关键词：Stroke;Shoulder hand syndrome;Clinical effect;Acupuncture along meridian299|635|0更新时间：2023-08-11
- 摘要：Objective To investigate the therapeutic effect of scalp acupuncture and rehabilitation training on balance dysfunction in children with spasmodic hemiplegia so as to provide the reference to the optimization of treatment scheme. Me-thods A total of 60 children with spastic hemiplegia were divided into a routine group and a scalp acupuncture group, 30 cases in each one according to random number table. In the routine group, the rehabilitation training was provided, including exercise training, balance training, spasmotherapy apparatus, electromyography biofeedback apparatus and orthoses. In the scalp acupuncture group, on the base of the treatment as the routine group, scalp acupuncture was supplemented at motor area, foot motor sensory area, equilibrium area and parietal temporal anterior oblique line. Separately, before the treatment, after 3 months treatment and after 6 months treatment, the dimension D and E of the gross motor function measure-88(GMFM-88) and Berg balance scale(BBS) were adopted to evaluate balance related motor functions and equilibrium function. The differences in the above 3 indicators at different time stages were compared in children between the two groups. Results Compared with the score before the treatment, BBS score was obviously increased after 3 and 6 months treatment in the patients of the two groups respectively(P<0.05). The score in the dimension D and E after 6-month treatment was increased significantly as compared with the score before treatment and after 3-month treatment in the same group respectively(P<0.05). Compared with the routine group, the score of dimension D and E of GMFM-88 as well as BBS score were all increased obviously in the scalp acupuncture group after 3 and 6 months treatment(P<0.05). Conclusion On the base of routine rehabilitation training, scalp acupuncture can improve balance function of children with spastic hemiplegia better.关键词：Cerebral palsy;Spastic hemiplegia;Balancing function;Scalp acupuncture;Rehabilitation training276|292|1更新时间：2023-08-11
- 摘要：Objective To observe the clinical effect of "metal hook fishing"(MHF)-like needling technique combined with Tuina manipulation in the treatment of scapulohumeral periarthritis. Methods A total of 72 patients with scapulohumeral periarthritis were randomly divided into MHF-like needling(MHF) and conventional needling groups(n=36 in each group). For patients of the GHF group, the doctor pressed the acupoint with the left index finger, held the disposable acupuncture needles to insert into the Jianqian, Jianyu(LI15), Jianliao(SJ5), Jianzheng(SJ9) and Ashi-point respectively with the right hand, waited for qi-arriving, twirled the needles forward about 3 to 6 times. When a tightened feeling was achieved beneath the needle tip, the doctor held the needle body to slightly shake it 3 to 6 times which mimicked a fish to swallow a hook, kept the situation for a while till relaxation, followed by retaining the needle for 30 min and then withdrew it slowly. Afterwards, the doctor performed Tuina manipulation at the upper limbs for 20 to 30 min. For patients of the conventional needling group, the same acupoints were needled and stimulated with the uniform reducing and reinforcing method for 1 min, followed by retaining the needles for 30 min. Then, massage was performed for 20 to 30 min in the same way as those in the MHF group. The treatment was conducted once daily, 6 times a week for 2 weeks. After the treatment, the visual analogue scale(VAS) was used to assess the pain degree, the range of motion(ROM) scale of the shoulder joint adopted to assess the function, and the activity of daily living(ADL) scale used to assess the patients' daily living ability. The therapeutic effects of the two groups were evaluated after the treatment. Results After the treatment, the VAS scores of the two groups were notably decreased(P<0.05), being significantly lower in the GHF group than in the conventional needling group(P<0.05). The ROM score and ADL score of the two groups were obviously increased(P<0.05), being significantly higher in the MHF group than in the conventional needling group(P<0.05). Of the 36 cases in the conventional needling and MHF groups, 7 and 17 were cured, 11 and 12 experienced remarkable improvement, 13 and 4 were improved, and 5 and 3 were ineffective, with the total effective rate being 86.1%(31/36) and 91.7%(33/36), respectively. The total effective rate of MHF group was evidently higher than that of the conventional needling group(P<0.05). Conclusion Both MHF and conventional needling is effective in relieving scapulohumeral periarthritis, and the effect of MHF is ob-viously superior to that of conventional needling in relieving pain state and improving function of the affected shoulder joint.关键词：Scapulohumeral periarthritis;Acupuncture;Metal hook fishing-like needling;Conventional needling274|722|0更新时间：2023-08-11
- 摘要：Objective To investigate the clinical effect of acupuncture combined with western medicine in the treatment of children with abdominal Henoch-Schonlein purpura with spleen-stomach damp-heat syndrome. Methods A total of 60 children with abdominal Henoch-Schonlein purpura with spleen-stomach damp-heat syndrome were randomly divided into treatment group and control group, with 30 patients in each group. The patients in the control group were given Hydroprednisone 2 mg·kg(-1)·d(-1)·d(-1), and in addition to the treatment in the control group, those in the treatment group were given acupuncture at Tianshu(ST25), Neiguan(PC6), Zusanli(ST36), Zhongwan(CV12), Qihai(CV6), and Sanyinjiao(SP6) once a day, with a needle retaining time of 15 minutes. Both groups were treated for 7 days. The scores of abdominal pain, hematochezia, hematemesis, vomiting, poor appetite, abdominal distension, purpura, occult blood in stool, and abdominal ultrasound were determined before and after treatment, and the time to the disappearance of abdominal pain was observed. Clinical outcome was evaluated. Results The treatment group had a significantly higher effective rate than the control group [96.7%(29/30) vs 80.0%(24/30), P<0.05]. Both groups had significant reductions in the scores of abdominal pain, hematochezia, hematemesis, poor appetite, abdominal distension, purpura, occult blood and abdominal ultrasound and the total score after treatment(P<0.05), and compared with the control group after treatment, the treatment group had significantly lower scores of abdominal pain, poor appetite, abdominal distension, and abdominal ultrasound and total score(P<0.05). The treatment group had a significantly shorter time to disappea-rance of abdominal pain than the control group(P<0.05). Conclusion Acupuncture combined with western medicine has a better clinical effect than western medicine alone in the treatment of abdominal Henoch-Schonlein purpura with spleen-stomach damp-heat syndrome and can significantly improve clinical symptoms and signs and shorten the time to disappearance of abdominal pain.关键词：Henoch-Schonlein purpura;Acupuncture;Abdominal pain371|364|1更新时间：2023-08-11
- 摘要：Chronic atrophic gastritis(CAG) is a common digestive disease in clinic. Previous experimental and clinical studies have shown that acupuncture has a positive effect for CAG. Apoptosis of gastric mucosal tissue has been shown to play an important role in the process of gastric atrophy and possibly further carcinogenesis in CAG, and the circular RNA(circRNA), a novel class of non-coding RNA, has been confirmed to play a regulatory role in the downstream pathway of apoptosis by many stu-dies. Accumulated findings of experimental studies showed that acupuncture and moxibustion interventions could suppress apoptosis of the cultured human gastric mucosal epithelial cells and lower apoptotic index of gastric mucosal cells in CAG rats. Therefore, circRNA is likely to mediate the inhibitory effect of acupuncture and moxibustion on apoptosis of gastric mucosal epithelial cells in CAG. In this paper, we systematically summarized 1) the regulation of circRNA on apoptosis, 2) the apoptosis and pathological mechanism of CAG, 3) the effect of acupuncture on apoptosis, and proposed that circRNA is highly likely to be involved in the positive effect of acupuncture and moxibustion interventions for CAG. It is recommended that researches should further reveal the scientific basis of acupuncture and moxibustion therapies in the treatment of CAG by exploring the role of related circRNAs and their downstream target proteins in the gastric mucosal tissues.关键词：Chronic atrophic gastritis;Acupuncture-moxibustion;Gastric mucosal tissue;Apoptosis;Circular RNA345|667|2更新时间：2023-08-11
- 摘要：Nonspecific low back pain(NLBP) is a heterogeneous disease with no definite pathological anatomical causes. Although acupuncture treatment of NLBP has been included in the international clinical guidelines for low back pain in many countries, the grade strength is still low, and the results of clinical efficacy evaluation are controversial. Moderate evidence from international studies indicates that acupuncture treatment of NLBP has a clear short-term analgesic effect and can improve pain related depression symptoms to a certain extent, but the exploration of efficacy rules is relatively insufficient, and there is no specific operational scheme to guide the acupuncture treatment of NLBP. Domestic clinical studies are quite distinctive in the aspects of dose-effect acupoint selection, characteristic needling methods, and acupuncture apparatuses, but lack of systematic research, and have low quality and weak evidence. In terms of the underlying mechanisms, acupuncture may achieve analgesic effect through anti-inflammation, relieving central sensitization, and improving the damage of local multifidus muscles and bones. However, due to the heterogeneity of NLBP disease, the current elaboration on the mechanism of acupuncture treatment of NLBP may not reach a consensus, and it is still worth further exploration.关键词：Acupuncture;Nonspecific low back pain;Clinical efficacy;Mechanism335|1825|4更新时间：2023-08-11
- 摘要：Histological pictures are important parts of medical research articles, and a good histological picture not only visualizes the content of the article, but also makes it easier for readers to understand. With the rapid development of microscopic technology and image processing technology, journals have higher requirements for the quality of histological pictures and more strict requirements for the editing and annotation of pictures. Based on an analysis of the current status of histological pictures in the articles of Acupuncture Research, this article proposes our suggestions and methods for rational use of histological pictures, so as to provide a reference for improving the quality of histological pictures in the articles published in Acupuncture Research.关键词：Acupuncture-moxibustion;Histology;Picture;Image processing;Microscope285|135|1更新时间：2023-08-11
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