最新刊期
卷 46 ， 期 3 ， 2021
- 摘要：Objective To observe the effect of electroacupuncture preconditioning at "Zusanli"(ST36,Lower Confluent point) and "Zhongwan"(CV12,Front-Mu point) combination on oxidative stress and inflammation-related indicators, Toll-like receptor 4(TLR4), myeloid differentiation factor 88(MyD88) and inhibitor-α of nuclear transcription factor κB(IκB-α) in serum and gastric tissue of rats with stress gastric ulcer(SGU),so as to explore its mechanisms underlying prevention of SGU. Methods A total of 36 Wistar rats were randomly divided into blank control, model, positive drug and He-Sea-Front-Mu point combination groups(n=9 in each group). A rat model of SGU was established by restraint water-immersion stress method. Ten days before mode-ling, rats in the He-Sea-Front-Mu point combination group received electroacupuncture(2 Hz, 0.6 mA)at ST36 and CV12 for 10 min once every other day for 10 days, and those in the positive drug group was treated by gavage of omeprazole(20 mg/kg) once every other day for 10 days. The morphology of the gastric mucosa was observed by naked eyes and hematoxylin-eosin staining, and the ulcer index(UI) and lesion score were calculated. TBA and colorimetric methods, ELISA and Western blot were used to detect malondialdehyde(MDA), myeloperoxidase(MPO), glutathione peroxidase(GSH-Px), tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) levels and the relative expressions of TLR4, MyD88, and IκB-α protein, separately. Results The gastric mucosa of rats in the blank control group was smooth and intact, the cells were arranged neatly, and there was no telangiec-tasia, hyperemia and inflammatory cell infiltration. The gastric mucosal epithelial structure of rats in the model group was destroyed, and a large number of mucosal epithelial cell death and inflammatory cell infiltration were seen. The degree of gastric mucosal injury and inflammatory cell infiltration in the positive drug group and the combined point group was less than that in the model group. Compared with the blank control group, the UI and lesion score of rats in the model group were significantly increased(P<0.05), the levels of MDA and MPO in the serum and gastric tissues were significantly increased(P<0.05), GSH-Px was significantly reduced(P<0.05), the contents of TNF-α and IL-6 in serum were markedly increased(P<0.05), the expression levels of TLR4 and MyD88 proteins in gastric tissue were significantly increased(P<0.05), IκB-α was significantly reduced(P<0.05). After intervention and in comparison with the model group showed that, the UI and lesion score, the levels of MDA and MPO, contents of serum TNF-α and IL-6, expression levels of TLR4 and MyD88 proteins in positive drug and He-Sea-Front-Mu point combination groups were significantly decreased(P<0.05), while GSH-Px and IκB-α were significantly increased(P<0.05); There were no significant differences in the above indicators between the positive drug and the He-Sea-Front-Mu point combination groups(except TNF-α). Conclusion Electroacupuncture preconditioning at ST36 and CV12 can prevent SGU, which may be related to its effects in anti-oxidant, anti-inflammatory and regulating TLR4/MyD88/IκB signaling pathway.关键词：Stress gastric ulcer;Electroacupuncture;Oxidative stress;Inflammation38|664|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) at "Zusanli"(ST36) on the expression of epidermal growth factor receptor(EGFR), tumor necrosis factor α(TNF-α) transfer growth factor α(TGF-α), interleukin-8(IL-8), p38 mitogen-activated protein kinases(p38 MAPK), mucin-5 AC(MUC5 AC) and other related factors in chronic obstructive pulmonary disease(COPD) rats, so as to reveal its underlying mechanisms in improving COPD. Methods A total of thirty male SD rats were randomly divided into normal control, model and EA groups, with 10 rats in each group. The COPD model was replicated using a combined method of tracheal infusion of lipopolysaccharide(LPS) and forced smoke-inhaling. EA(1—3 mA, 4 Hz/20 Hz) was applied to bilateral ST36 for 30 min, once daily for two consecutive weeks. The lung ventilation activities including the forced vital capacity(FVC) and forced expiratory volume(FEV) at 0.1 and 0.3 s(FEV0.1, FEV0.3) were detected. Histopathological changes of the middle lobe and bronchus of the right lung were observed after H.E. staining. The contents of TGF-α, TNF-α and IL-8 in the serum, bronchoalveolar lavage fluid(BALF) and superior lobe of the right lung were assayed by using ELISA, and the expression levels of EGFR, p38 MAPK and MUC5 AC proteins(inferior lobe of the left lung) and mRNAs(inferior lobe of the right lung) detected using Western blot, immunohistochemistry(strept avidin-biotin complex, SABC method) and real-time quantitative PCR, respectively. Results Compared with the normal group, the FVC, FEV0.1, FEV0.3, FEV0.1/FVC and FEV0.3/FVC levels were significantly decreased(P<0.01), while the contents of TNF-α, TGF-α and IL-8 in the serum, BALF and lung tissues, expression levels of EGFR, p38 MAPK and MUC5 AC mRNAs and proteins, and the immunoactivity of EGFR, p38 MAPK and MUC5 AC in the lung tissues were significantly increased in the model group(P<0.01). After EA intervention, the decreased levels of the FVC, FEV0.1, FEV0.3, FEV0.1/FVC and FEV0.3/FVC, and the increased levels of the abovementioned genes and proteins were all reversed in the EA group(P<0.01, P<0.05). After modeling, the bronchial walls were thickened, with enlarged alveolar cavities, fractured alveolar walls, obvious inflammatory cell infiltration, and rich mucus secretion in the lumen, which was relatively milder in the EA group. Conclusion EA of ST36 can improve the ventilation function in COPD rats, which may be associated with its function in down-regulating the levels of TNF-α, TGF-α, IL-8, EGFR, p38 MAPK and MUC5 AC mRNAs and proteins in the lung tissues, inhibiting EGFR-p38 MAPK signaling mediated expression of MUC5 AC.关键词：Electroacupuncture;Chronic obstructive pulmonary disease;Epidermal growth factor receptor(EGFR)-p38MAPK signaling;Mucin-5AC26|359|5更新时间：2023-08-11
- 摘要：Objective To investigate the effect of electroacupuncture(EA) at "Taichong"(LR3) on blood pressure, sympathetic nerve activity, baroreflex sensitivity, and α 2-adrenergic receptor(α2 AR) expression in nucleus tractus solitarii(NTS) in hypertensive rats, so as to reveal its mechanisms underlying improvement of hypertension. Methods Male Sprague Dawley rats were randomly divided into 4 groups: sham-operation, model, EA, and sham-EA(non-acupoint) groups, with 12 rats in each group. The hypertension model was established by occlusion of the right renal aorta(two-kidney-one clip method). Rats of the sham-operation group received the same surgery but without occlusion of the renal artery. EA(2 Hz/15 Hz, 2 mA) was applied to bilateral LR3 for 30 min, once a day for 28 days, and sham EA was applied to the skin of the rat tail near the buttock on both sides. The mean arterial pressure(MAP) of the abdominal aorta and heart rate(HR) were recorded. The autonomic nerve function was assessed by using frequency domain analysis of heart rate variability(HRV), and the baroreflex sensitivity detected by sequential method. Plasma norepinephrine(NE) level was measured by ELISA, and the α2 AR positive neurons and α2 AR protein expression in NTS were detected by using fluorescence immunohistochemistry and Western blot, respectively. The functions of α2 AR within the NTS in modulating MBP and HR were verified by microinjection of its agonist(clonidine) and antagonist(yohimbine) separately.Results Compared to the sham operation rats, the hypertension rats displayed significant increases in the MAP(P<0.01), plasma norepinephrine content(P<0.01), ratios of low frequency/total power(LF/TP) and low frequency/high frequency(LF/HF)(P<0.01), and significant reduction in the overall gain, uplink sequence gain and downlink sequence gain of baroreflex(P<0.01), the number α2 AR positive neurons and α2 AR protein expression level in NTS(P<0.01). The rats in the EA group(rather than in the sham-EA group) showed significant reduction in MAP at the 3 rd and 4 th week, plasma NE content, LF/TP and LF/HF(P<0.01), and obvious increase in the overall gain, uplink sequence gain and downlink sequence gain of baroreflex(P<0.01), and the number of α2 AR positive neurons and α2 AR protein expression in comparison(P<0.05) with those of the model group. Microinjection of clonidine into NTS induced an evident decrease in both MAP and HR in the model group relevant to the sham operation group(P<0.01), while the MAP and HR changes of the EA(not sham EA) group were considerably bigger than those of the model group(P<0.05), being similar to those of the sham-operation group(P>0.05), which suggested an elimination of the BP-lowering effect of clonidine after EA. Conclusion EA at LR3 can reduce MBP, sympathetic activities, improve baroreflex sensitivity in renovascular hypertensive rats, which may be associated with its effects in up-regulating the decreased NTS α2 AR expression and functional activities.关键词：Electroacupuncture;Hypertension;α2-adrenergic receptor;Nucleus tractus solitarii;Baroreflex sensitivity27|354|2更新时间：2023-08-11
- 摘要：Objective To explore the effect of moxibustion at "Zusanli"(ST36) and "Shenshu"(BL23) on synovitis, and expressions of miR-155, Toll-like receptor 4(TLR4), myeloid differentiation factor 88(MyD88), interlukine(IL-1) receptor-associated kinase(IRAK1), tumor necrosis factor receptor-associated factor 6(TRAF6), nuclear factor κB(NF-κB), IL-1β, tumor necrosis factor receptor(TNF)-α and IL-6 mRNA and protein of synovial membrane in rheumatoid arthritis(RA) rats, so as to explore its mechanism underlying improvement of RA. Methods A total of 48 male Wistar rats were randomly divided into normal control, model, moxibustion and antagonist groups(n=12 rats in each group). The RA model was replicated by placing the rats in a wind, cold and wet environment and injection of Freund's complete adjuvant(CFA, 0.5 mL) into the right hindlimb foot plantar. Moxibustion was applied to bilateral ST36 and BL23 for 30 min, once daily for 21 consecutive days. Rats of the antagonist group was treated by injection of TLR4 antagonist(TAK-242, 1 mg/mL, 0.1 mg/kg) via tail vein, once per day for consecutive 21 d. The joint swelling degree(JSD) and arthritis index(AI, red swelling scale) were determined, and the expression levels of various indicators of miR-155, and TLR4, myeloid MyD88, IRAK1, TRAF6, NF-κB, IL-1β, TNF-α and IL-6 mRNA and protein were assayed by quantitative real time-PCR and Western blot, separately. Results Compared with the normal control group, the JSD and AI, and the expression levels of synovial miR-155, TLR4, MyD88, IRAK1, TRAF6, NF-κB, IL-1β, TNF-α and IL-6 mRNA and protein were significantly increased in the model group(P<0.01). Compared with the model group, the increased levels of JSD and AI, and the expression levels of synovial miR-155, TLR4, MyD88, IRAK1, TRAF6, NF-κB, IL-1β, TNF-α and IL-6 mRNA and protein were notably down-regulated in both moxibustion and antagonist groups(P<0.01). The effects of moxibustion were evidently superior to the antagonist in down-regulating the abovementioned indexes(P<0.01), except TLR4 mRNA and protein. Conclusion Moxibustion at ST36 and BL23 can reduce the synovitis of RA rats, which is related to its effects in suppressing the expressions of miR-155, TLR4, MyD88, IRAK1, TRAF6, NF-κB, IL-1β, TNF-α and IL-6 mRNA and protein(i.e., inhibition of miR-155/TLR4/NF-κB signaling).关键词：Moxibustion;Adjuvant arthritis;MiR-155;TLR4/NF-κB signaling pathway40|701|19更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupoint catgut embedding on intestinal mucosal epithelial injury, and expression of epithelial tight junction proteins and mRNAs and protein kinase C(PKC) protein(intestinal mucosal epithelial barrier-related molecules) in rats with experimental ulcerative colitis(UC) of "deficiency-stasis" type, so as to explore its mechanisms underlying improvement of UC. Methods Male SD rats were randomly divided into normal control, UC model, medication(sulfasalazine, SASP) and catgut-embedding groups. The "deficiency-stasis" type UC model was established by gavage of adenine(10 mL/kg) for 2 weeks, cold Folium Sennae(10 mL/kg) for 2 weeks, and then enema of mixture solution of 5% trinitro-benzene-sulfonic acid(100 mg/kg) + 50% ethyl alcohol. Rats of the medication group received gavage of SASP. The catgut-embedment was applied to bilateral "Tianshu"(ST23),"Zusanli"(ST36), "Geshu"(BL17), "Pishu"(BL20), "Shenshu"(BL23) and "Dachangshu"(BL25), once every two weeks, 3 times altogether. The body mass was recorded, and histopathological changes of the colon tissues were observed after H.E. staining. The contents of serum D-lactic acid(D-LA), diamine oxidase(DAO) and protein kinase C(PKC) were detected by ELISA. The expression levels of occludin, claudin-8, cingulin and zonulin mRNAs and proteins and PKC protein of the colon tissues were detected by using quantitative real-time PCR and Western blot, separately. Results Compared with the control group, the body mass and expression levels of colonic occludin, claudin-8 and cingulin mRNAs and proteins were significantly lower(P<0.01), and the colonic mucosa damage index(CMDI) score, contents of serum D-LA, DAO and PKC, as well as the expression levels of zonulin mRNA and protein and PKC protein were significantly higher in the model group(P<0.01). In comparison with the model group, the body mass, and the expression levels of occludin, claudin-8 and cingulin mRNAs and proteins were significantly higher(P<0.05, P<0.01), whereas the CMDI score, expression levels of zonulin mRNA and protein and PKC protein were remarkably lower in both the medication group and acupoint embedding groups(P<0.05,P<0.01). No significant differences were found between the acupoint embedding and medication groups in all the indexes mentioned above(P>0.05). H.E. staining showed markedly swollen, disordered arrangement of intestinal mucosal cells, and hemorrhage with infiltration of inflammatory cells in the colonic tissues after modeling, which was relatively milder in both medication and acupoint embedding groups. Conclusion Acupoint catgut embedding can reduce colonic tissue injury in UC rats, which may be related to its functions in regulating the expression of intestinal epithelial tight junction proteins and mRNAs and in inhibiting the activation of PKC protein.关键词：Acupoint catgut embedding;Ulcerative colitis;Intestinal mucosal epithelial barrier;Tight junction protein;Protein kinase C38|462|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the behavior, histomorphology and the expression of angiopoietin-1(Angpt-1) in rats with spinal nerve injury, so as to explore its mechanism on neuropathic pain. Methods Forty-five male SD rats were randomly divided into sham, model and EA groups(n=15 rats in each group). Spinal nerve ligation(SNL) of the L5 lumbar vertebra was performed to establish a rat model of neuropathic pain. The rats in the EA group were given EA at "Zusanli"(ST36) and "Kunlun"(BL60) of the operation side with continuous wave at a frequency of 2 Hz and an intensity of 1.5 mA once a day, 30 minutes each time for 7 days. The sham group only exposed L5 spinal nerves without ligation. Mechanical withdrawal threshold(MWT) and thermal withdrawal latency(TWL) were observed and recorded before modeling and on days 3,5,7,10,12 and 14 after modeling. L4—L6 segments of spinal cord were taken and the morphological changes of spinal dorsal horn were observed by HE staining. The changes of spinal dorsal horn nerve fiber structure were observed by silver plating staining. Angpt-1 expression was detected by Western blot and immunohistochemistry. Results Compared with the sham group, the model group had significant reductions in MWT and TWL at each time point(P<0.01); compared with the model group, the EA group had significant increases in MWT and TWL on days 10,12 and 14 after intervention(P<0.05, P<0.01). HE staining showed that in the model group, the spinal dorsal horn showed degeneration and necrosis of neurons, nuclear fixation and shrinkage, and loose surrounding tissues. The degree of tissue damage of the EA group was milder than that of the model group. The silver staining results showed the model group had obvious neuronal fibrillary tangles, while there were fewer neuronal fibrillary tangles in the EA group. Compared with the sham group, the Angpt-1 expression in the model group was significantly decreased(P<0.01), and compared with the model group, the EA group had a significant increase in the expression of Angpt-1(P<0.01). Conclusion EA can promote the recovery of nerve function in SNL rats by up-regulating Angpt-1 expression.关键词：Neuropathic pain;Electroacupuncture;Angiopoietin-1;Nerve repair29|276|1更新时间：2023-08-11
- 摘要：Objective To explore the protective effect and molecular mechanism of electroacupuncture(EA) preconditioning on renal injury induced by streptozotocin(STZ) in hyperglycemic mice. Methods Eighty male C57 BL/6 mice were randomly divided into 4 groups: control, model, EA and sham EA groups, n=20 in each group. The hyperglycemic model was established by intraperitoneal injection of 0.1% STZ solution(50 mg/kg) for 5 days. EA preconditioning or sham EA was applied at "Zusanli"(ST36) and "Shenshu"(BL23), once daily for 7 successive days in the EA or sham EA group. Three days after mode-ling, the blood glucose was measured after fasting for 6 hours. The degree of renal injury was observed by HE staining and PAS staining; the expressions of transient receptor potential cation channel 6(TRPC6) and Nephrin protein in glomerulus were observed by immunohistochemistry; the expressions of TRPC6 and Nephrin protein in renal cortex were detected by Western blot. Results Compared with the control group, the blood glucose level was significantly increased(P<0.01), the glomerular cross-sectional area was apparently increased(P<0.000 1), the glomerular capillaries dilated, the matrix proliferated, the brush edge of renal tubules disappeared, the proportion of abnormal renal tubules increased(P<0.000 1), and the expressions of TRPC6 and Nephrin in glomeruli and renal tissue were significantly increased(P<0.01) in the model group. Following EA treatment and compared with the model and sham EA groups, the blood glucose was significantly down-regulated(P<0.01), the renal tissue injury was apparently alleviate, the cross-sectional area of glomerulus was reduced(P<0.05), the brush edge of renal tubules changes were obviously improved, the proportion of abnormal renal tubules decreased(P<0.000 1), and the expressions of TRPC6 and Nephrin in glomerulus and renal tissue decreased(P<0.05) in the EA group. Conclusion EA preconditioning can alleviate renal injury in hyperglycemic mice, which is closely related to its effects in reducing renal TRPC6 and Nephrin expressions and inhibiting podocyte activation.关键词：Electroacupuncture preconditioning;Hyperglycemia;Transient receptor potential channel protein 6;Renal injury24|240|2更新时间：2023-08-11
- 摘要：Objective To observe the effect of proximal and distal acupoint catgut-embedding on the contents of prostaglandin E_2(PGE_2) and prostaglandin F_(2α)(PGF_(2α)) in the uterus, serum Interleukin-2(IL-2) contents and splenic natural killer(NK) cell activity in primary dysmenorrhea(PD) rats, so as to explore the different effects of proximal and distal acupoint catgut-embedding on PD rats. Methods Female Wistar rats were randomly divided into control, model, proximal catgut-embedding and distal catgut-embedding groups, with 8 rats in each group. The PD model was established by subcutaneous injection of estradiol ben-zoate(0.5 mg/rat on the first day and 10(th) day, and 0.2 mg/rat from the 2(th) day, and 0.2 mg/rat from the 2(nd) to the 9(nd) to the 9(th) day) and intraperitoneal injection of oxytocin(2 U/rat, on the 11(th) day) and intraperitoneal injection of oxytocin(2 U/rat, on the 11(th) day). Catgut-embedding was applied at bilateral "Guanyuan"(CV4) and "Ciliao"(BL32) in the proximal catgut-embedding group, and bilateral "Sanyinjiao"(SP6) and "Diji"(SP8) in the distal catgut-embedding group. After the treatment, the body writhing times in 30 min and uterine mass were recorded, PGE_2 and PGF_(2α)in uterus and IL-2 in serum were assayed by using ELISA, the activity of NK cell in the spleen was detected using MTT colorimetry. Results Following modeling, the body writhing times of the model group was increased than that of the control group(P<0.01); After interventions, the body writhing times were decreased in the proximal and distal catgut-embedding groups than those of the model group(P<0.01). Compared with the control group, the uterine mass and uterus PGF_(2α)content were increased(P<0.01), while uterus PGE_2, serum IL-2 and splenic NK cell activity were decreased(P<0.01) in the model group. After interventions, the uterine mass and uterus PGF_(2α) were down-regulated(P<0.01, P<0.05), while the contents of uterus PGE_2, serum IL-2 and splenic NK cell activity up-regulated(P<0.01) in the proximal and distal catgut-embedding groups in contrast to the model group. The content of uterus PGF_(2α) was down-regulated(P<0.05) and splenic NK cell activity was up-regulated(P<0.01) in the proximal catgut-embedding group than those of the distal catgut-embedding group. Conclusion Both proximal and distal acupoint catgut-embeding can increase PGE_2 and decrease PGF_(2α) in the uterus, increase the level of serum IL-2 and the activity of NK cells in the spleen in PD model rats, thereby achieving analgesic effect. The effect of proximal catgut-embedding is better than that of distal catgut-embedding.关键词：Primary dysmenorrhea;Proximal catgut-embedding;Distal catgut-embedding;Prostaglandin E_2;Prostaglandin F_(2α);Splenic NK cell activity28|655|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion on the expression of phosphorylated calcium/calmodulin-dependent protein kinase Ⅱα(pCaMKⅡα) and neuronal nuclei(NeuN) and the ability of learning and memory in the neonatal mice model of hypoxic-ischemia encephalopathy(HIE), so as to explore its mechanism underlying improvement of learning and memory. Methods ICR mice(aged 7 days) were randomly divided into sham operation, model and moxibustion groups. HIE model was induced by ligation of the right common carotid artery combined with hypoxia in a closed transparent chamber. Mice in the moxibustion group were treated with gentle moxibustion at "Dazhui"(GV14) for 35 min, once daily for 3 consecutive days. The pathological changes of brain tissues were observed with the naked eyes and under microscope after H.E. staining, respectively. The expressions of pCaMKⅡα and NeuN in the ischemic penumbra were examined by immunofluorescent staining, and the learning and memory ablility was tested with Morris maze. Results No infarcts were found in the brain tissue of the mice in the sham operation group. Compared with the sham operation group, mice in the model group had infarcts and the expression of pCaMKⅡα and NeuN in the ischemic penumbra was significantly reduced(P<0.01), and the latency to find a platform was significantly prolonged in Morris maze test(P<0.01). After moxibustion, in comparison with the model group showed that, small areas of infarction were seen in the right hemisphere of the moxibustion group, and the expressions of pCaMKⅡα, NeuN increased significantly(P<0.01), and the latency to find a platform was significantly shortened(P<0.01). Conclusion Moxibustion can improve the ability of learning and memory in the neonatal mice with HIE, which might be related to alleviating brain injury and increasing the expression of pCaMKⅡα in neurons of ischemic brain tissues.关键词：Hypoxic-ischemia encephalopathy;Moxibustion;Learning and memory;Phosphorylated calcium/calmodulin-dependent protein kinase Ⅱα;Neuronal nuclei36|260|0更新时间：2023-08-11
- 摘要：Objective To observe the effect of transcutaneous electrical acupoint stimulation(TEAS)combined with epidural analgesia on postpartum depression and to explore its underlying mechanism. Methods One hundred and twenty cases of full-term primiparous women with singleton pregnancy were selected from May 2018 to November 2018 in Jinzhong Maternal and Child Health Hospital. The parturients with labor analgesia requirement were randomly divided into the epidural group and the combination group, and the parturients without labor analgesia requirement were used as the control group, with 40 cases in each group. Patients in the control group did not receive labor analgesia and were treated according to the routine procedures of natural delivery; patients in the epidural group received epidural labor analgesia; patients in the combination group received TEAS at bilateral Hegu(LI4), Sanyinjiao(SP6) and Zusanli(ST36)(2 Hz/100 Hz, the current intensity is gradually increased from 15 mA, and the treatment was performed every 2 h, 20 min each time) combined with epidural labor analgesia. The visual analogue scale(VAS) scores were recorded when the uterine orifice opened to 3, 6, 8, 10 cm. Plasma glutamate was measured using high-performance liquid chromatography before analgesia, at the end of the third stage of labor and 42 days after delivery, and Edinburgh postnatal depression scale(EPDS) score was measured at 42 days after delivery.Results In comparison with the control group, the VAS score, EPDS score and the incidence of postpartum depression of the epidural group and the combination group were significantly lower(P<0.05), and the combination group had significant decrease than those in epidural group(P<0.05). Immediately before analgesia, there was no statistically significant difference in glutamate levels among the 3 groups(P>0.05). Compared with the control group, at the end of the third stage of labor and 42 days postpartum, the glutamate levels of the epidural group and the combination group were significantly reduced(P<0.05), and the combination group decreased more significantly than the epidural group(P<0.05). Conclusion TEAS combined with epidural analgesia can reduce the incidence of postpartum depression, possibly by down-regulating plasma glutamate level and relieving of labor pain.关键词：Transcutaneous electrical acupoint stimulation;Labor analgesia;Postpartum depression;Glutamate43|327|1更新时间：2023-08-11
- 摘要：Objective To explore the clinical effect of "Jin's three-needle" therapy and motor relearning regime for the recovery of upper limb motor function in the patients with ischemic stroke. Methods A total of 60 patients with ischemic stroke were randomly divided into an experiment group(30 cases) and a control group(30 cases). In the control group, the routine rehabilitation regimen was adopted. In the experimental group, on the base of the treatment regimen as the control group, the "Jin's three-needle" therapy was supplemented. The neurological function deficit score was used to assess the neurological function. The modified Fugel-Meyer assessment for upper extremities(FMA) and motor assessment scale(MAS) were adopted to assess the upper limb motor function in patients. The modified Barthel Index(MBI) was used to evaluate the activity of daily living(ADL) in patients. Results After treatment, the neurological function deficit score, the upper limb motor function(FMA and MAS) and ADL(MBI) were all improved obviously as compared with those before treatment(P<0.05). In the experimental group, the reducing range of neurological function deficit score(difference value) was larger than that in the control group(P<0.05). The increases of FMA score(upper arm function), the total score of MAS, the score for hand movement in MAS and the score for advanced hand activities of MAS, as well as MBI score in the experimental group were significantly larger than those in the control group successively(P<0.05). Conclusion "Jin's three-needle" therapy combined with exercise relearning regimen effectively reduces the degree of neurological deficit and improve the upper limb motor function and ADL in patients with ischemic stroke.关键词：Jin's three-needle therapy;Motor relearning regimen;Ischemic stroke;Upper limb motor function33|517|2更新时间：2023-08-11
- 摘要：Objective To investigate the application characteristics of electroacupuncture(EA) in the treatment of peripheral neuropathy, so as to provide a basis for clinical use of EA therapy. Methods Keywords of "electroacupuncture""peripheral neuropathy" "facial paralysis" "trigeminal neuralgia" "sciatica" "common peroneal nerve injury" "diabetic peripheral neuropathy" "intercostal neuralgia" "gluteal epithelial neuritis" "ulnar nerve injury" "median nerve paralysis" "postherpetic neuralgia", and "great occipital neuralgia" were used to search articles in both English and Chinese published from 1999 to 2019 in databases of CNKI, Wanfang, VIP, CBM, Ovid, PubMed and Embase and related books such as electroacupuncture, and neurology, followed by establishing a database of "Electroacupuncture Treatment of Peripheral Neuropathy". Then, the collected articles were put into statistical analysis after sorting, screening, input, checking, and data extracting by using data mining technology and statistical software EpiData. Results Of the searched 1 528 papers, 778 were eligible, involving 13 types of peripheral neuropathy which the facial paralysis and facial spasm were most frequently seen, followed by trigeminal neuralgia and sciatica, with an effective rate being above 90% for nearly all the 13 diseases. The acupoints employed were chiefly those close to the affected area and distribute along the nerve trunk. In addition, about the needling techniques, the penetration needling was frequently used, and the triple needling, quintuple needling and accompanied needling were also used. Regarding the related needle manipulations, the uniform reinforcing-reduction technique was most frequently used. The duration of EA was 30 min, with a highest stimulating frequency of 50 Hz. The acupoint injection was frequently supplemented, followed by moxibustion, and the treatment sessions were usually about 30 times. Conclusion EA therapy is frequently used in the treatment of peripheral neuropathy, and has some characteristics in acupoint selection, stimulating parameters and some additional needling techniques关键词：Electroacupuncture;Peripheral neuropathy;Data mining;Clinical application characteristic31|1401|5更新时间：2023-08-11
- 摘要：The concept of enhanced recovery after surgery(ERAS) is based on evidence-based medicine. By optimizing the treatment measures, the stress response of the body is reduced to meet the body's metabolic changes during the perioperative period, so as to achieve the purpose of accelerating recovery. In the perioperative period, acupuncture can relieve patients' preoperative anxiety, shorten fasting time, reduce the amount of anesthetics, protect organ function, reduce postoperative pain, reduce postoperative nausea and vomiting, and speed up postoperative recovery. The addition of acupuncture intervention in ERAS would provide new methods and new ideas for the construction of ERAS. But at present, the application of acupuncture in ERAS has problems such as lacking of overall consideration and insufficient standardization, etc.关键词：Enhanced recovery after surgery;Acupuncture;Transcutaneous electrical acupoint stimulation;Acupuncture anesthesia28|880|5更新时间：2023-08-11
- 摘要：Cupping therapy is an important part of traditional Chinese medicine, and its efficacy has been gradually accepted and confirmed by modern clinical medicine, but its physiological mechanisms have no consensus. In the present paper, we summarized the related mechanisms from results of both domestic and international researches which include 1) blood circulation promotion theory, 2) neurological reflex theory, 3) gate theory of pain, 4) inflammation-immune reaction theory and 5) skin tension increase theory. We also reviewed progresses of clinical researches about application of cupping therapy to cardiovascular diseases, chronic musculoskeletal pain, migraine and other painful symptoms, as well as arthritis which effects involve the above mentioned blood-circulation promotion, tension-increase, gate theory and inflammation-immune reaction, respectively. We believe that the mechanisms of cupping therapy are not independent and should be explored further from different perspectives.关键词：Cupping therapy;Physiological mechanism;Clinical application;Blood circulation promotion32|1147|4更新时间：2023-08-11
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