最新刊期
卷 46 ， 期 9 ， 2021
- 摘要：Objective To observe the effect of electroacupuncture(EA)on the expression of myelin basic protein(MBP), axon growth inhibitor Nogo-A and Nogo receptor(NgR) in corpus callosum of rats with focal cerebral ischemia, so as to explore the mechanism of EA underlying improving ischemic white matter injury. Methods Fourty male SD rats were randomly divided into normal, sham operation, model and EA groups, with 10 rats in each group. The focal cerebral ischemia rat model was established by occlusion of the middle cerebral artery(MCAO). EA was applied to “Baihui”(GV20) and “Zusanli”(ST36) on the left side for 30 min, once daily for 14 days. Neurological function score and the adhensive removal test were used to evaluate neurological deficit severity; Hematoxylin-esion staining was used to observe the pathological changes in myelin of corpus callosum and luxol fast blue(LFB) staining was used to observe the myelin of corpus callosum. Immunohistochemical staining and Western blot were used to detect the expressions of MBP、Nogo-A and NgR in the ischemic corpus callosum.Results After MCAO, the neurological function score was significantly increased(P<0.05), the time required for contact with tape and tape removal was longer(P<0.001), the intensity of LFB staining and the expression of MBP decreased, while the veside area and the expression of Nogo-A and its receptor NgR increased(P<0.01, P<0.05) in the model group relevant to the normal and sham operation groups. The fiber arrangement of the corpus callosum on the ischemic side was disordered and a large amount of myelin sheath was lost in the model group. Following the treatment, the neurological deficit score of EA group was gradually decreased and significantly decreased on the 3(rd), 7(rd), 7(th) and 14(th) and 14(th) day(P<0.05), and the time to remove the adhesive tape was shortened at the 7(th) day(P<0.05), and the time to remove the adhesive tape was shortened at the 7(th) and 14(th) and 14(th) day(P<0.001). The shape of the corpus callosum in the EA group was close to normal, and the myelin structure was relatively complete. The intensity of LFB staining and the expression of MBP was increased(P<0.05, P<0.01) while the expression of Nogo-A and its receptor NgR were decreased in the EA group relevant to the model group(P<0.01). Conclusion EA can play a protective role in myelin of the corpus callosum after cerebral ischemia, which may be related to down-regulating the expressions of Nogo-A and NgR.关键词：Electroacupuncture;Cerebral ischemia;Corpus callosum;Myelin basic protein;Axon growth inhibitor24|478|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on urodynamics of neurogenic bladder and pituitary adenylate cyclase activating peptide(PACAP)/cyclic adenosine monophosphate(cAMP)/protein kinase A(PKA) signaling pathway in detrusor tissue of rats after suprasacral spinal cord injury(SCI), so as to explore its possible mechanism in improving detrusor hyperreflexia bladder function after shock stage of suprasacral SCI. Methods Female SD rats were randomly divided into control, sham operation, model and EA groups, with 12 rats in each group. T10 spinal cord transection(SCT) was performed by surgery. Rats in the EA group were given EA(10 Hz/50 Hz, 20 min) at “Ciliao”(BL32), “Zhongji”(CV3) and “Sanyinjiao”(SP6) once daily for 7 days. After the intervention, urodynamics testing was detected in each group. HE staining was used to observe the morphological changes of bladder detrusor. The protein and mRNA expression of PACAP38 in detrusor was detected by immunohistochemistry, Western blot and real-time quantitive PCR, respectively. The contents of cAMP and PKA were determined by ELISA. Results Compared with the control and sham operation groups, the maximum bladder capacity and bladder com-pliance, and the protein and mRNA expression of PACAP38, and the contents of cAMP and PKA of the model group were significantly decreased(P<0.01, P<0.05), while the base pressure and leakage point pressure of bladder were significantly increased(P<0.01). After EA intervention, the above indexes were all reversed in the EA group relevant to those of the model group(P<0.01, P<0.05). Conclusion EA at BL32, CV3 and SP6 can improve the bladder function in rats with bladder detrusor hyperreflexia after SCI, which may be related to its effect in activating the PACAP/cAMP/PKA signaling pathway in detrusor tissue.关键词：Electroacupuncture;Neurogenic bladder;Pituitary adenylate cyclase activating peptide;Cyclic adenosine monophosphate;Protein kinase A;Detrusor24|986|6更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the expression of Toll like receptor 4(TLR4)and heat shock protein 90(HSP90) in the spinal cord of rats with chronic constriction injury(CCI) of sciatic nerve, so as to explore the mechanism of spinal cord TLR4 and HSP90 in alleviating chronic neuropathic pain by EA. Methods Male Wistar rats were randomized into control, model, EA, HSP90 inhibitor(inhibitor) and EA+ inhibitor groups(n=10 in each group). The neuropathic pain model was established by ligature of the right sciatic nerve to induce CCI. EA(1 mA,2 Hz/15 Hz)was applied at bilateral “Zusanli”(ST36) and “Yanglingquan”(GB34) for 30 min, once daily for 5 days. Rats of the inhibitor and EA+inhibitor groups were given a subcutaneous injection of HSP90 inhibitor geldanamycin(50 μg/kg) at the neck before daily EA. The paw withdrawal latency(PWL) of the bilateral hind-limbs was detected by using an algesia-detector. The contents of interleukin 1β(IL-1β) and tumor necrosis factor α(TNF-α) in the lumbar spinal cord(L2—L4) tissue were detected by enzyme-linked immunosorbent assay. The relative expression levels of HSP90 and TLR4 proteins in the lumbar spinal cord(L2—L4) were detected using Western blot and immunofluorescence double labeling, respectively. Results Following CCI, a strong thermal hyperalgesia, an apparent up-regulation of expression of HSP90 and TLR4 proteins and TLR4 in microglia, and increasing levels of IL-1β and TNF-α in the spinal cord were induced in the model group relevant to the control group(P<0.01,P<0.05). Five sessions of EA intervention or inhibitor injection significantly attenuated hyperalgesia, reversed the increase of IL-1β and TNF-α, and down-regulated the expression of TLR4 in microglia(P<0.05). Compared with the model group, the expression of HSP90 was further increased(P<0.05), and those of TLR4 in microglia and neurons were significantly decreased and increased, respectively in the EA group(P<0.05). Compared with the EA group, the levels of PWLD,TLR4 and HSP90 expression, and the proportions of neuronal nuclei antigen(NeuN) and TLR4, and ionized calcium binding adapter molecule(Iba1) and TLR4 co-expressed cells were significantly decreased in the inhibitor group and EA+inhibitor group(P<0.05). The proportion of NeuN and TLR4 co-expression cells in the EA+inhibitor group was significantly higher than that of the inhibitor group(P<0.05). Conclusion EA stimulation of ST36 and GB34 can alleviate thermal hyperalgesia in CCI rats, which is closely associated with its effect in regulating the expression of TLR4 in the spinal cord neurons and microglia. HSP90 in the spinal cord may be a co-stimulatory molecule for EA induced relief of neuropathic pain by regulating TLR4.关键词：Chronic neuropathic pain;Acupuncture analgesia;Toll-like receptor 4;Heat shock protein 90;Microglia;Neuron22|548|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of Mongolian medicine three-acupoints balance needling on the expression of p11/tPA/BDNF pathway and miRNA-16 in the hippocampus and middle raphe nucleus(MRN) in chronic stress depression model rats, so as to explore its mechanisms underlying improvement of depression.Methods Male SD rats were randomly divided into blank control, model, medication and Mongolian medicine acupuncture(acupuncture) groups, with 12 rats in each group. The depression model was established by using chronic unpredictable mild stress method. The rats in the medication group received gavage of prozac(2 mg/kg, diluted with normal saline, 1 mg/mL) 1 h after stress stimulation, once per day for 28 days, and those in the acupuncture group received three-acupoints(Heyi, Badagan and Xin) needling, once a day for 28 days. The behavioral changes were detected by using open field test and sugar consumption test before modeling and after the intervention. The immunoactivity of p11 and tPA proteins in the MRN, and their expression levels in both the MRN and hippocampus were detected by using immunofluorescence histochemistry and Western blot, separately, and the expression levels of miRNA-16 and BDNF mRNA in the hippocampus and MRN detected by using real-time quantitative PCR.Results After modeling, the crossing and rearing scores of open field tests and the relative consumption of sucrose in the model group were apparently lower than those of the blank control group(P<0.05), the expression levels of p11 and tPA in the MRN, and those of p11 and tPA proteins and BDNF mRNA in the MRN and hippocampus were significantly down-regulated(P<0.05), while those of miRNA-16 in the hippocampus and MRN were significantly up-regulated(P<0.05). Compared with the model group, the crossing and rearing scores of open field tests and glucose consumption, as well as the expression levels of p11 and tPA proteins and BDNF mRNA in the hippocampus and MRN were obviously increased in both the medication and acupuncture groups(P<0.05), while the expression of miRNA-16 in hippocampus was markedly down-regulated in both the medication and acupuncture groups(P<0.05). No significant differences were found between the acupuncture and medication groups in all the indexes mentioned above(P>0.05). Conclusion Mongolian medicine three-acupoints balance needling can improve the depressive state in depression rats, which may be associated with its effects in up-regulating the expression of p11 and tPA proteins and BDNF mRNA in the hippocampus and MRN and in down-regulating miRNA-16 in the hippocampus, suggesting an involvement of miRNA-16 controlled p11/tPA/BDNF signaling pathway in the antidepressant effect of acupuncture.关键词：Depression;Mongolian medicine three-acupoints balance needling;Hippocampus;Middle raphe nucleus;p11/tPA/BDNF pathway;miRNA-1626|393|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture on the expression of vascular endothelial growth factor(VEGF), basic fibroblast growth factor(bFGF) and endostatin(Endostatin, ES) mRNAs and proteins(angiogenesis related factors) in the ischemic penumbra region in rats with cerebral infarction(CI), so as to explore its underlying mechanisms in prolonging the time window of thrombolysis therapy for CI. Methods A total of 48 male SD rats were randomly divided into sham operation, model, medication(6 h thrombolysis) and acupuncture(Acupunct)+medication groups(n=12 in each group). The CI model was established using modified auto-thrombus method. Six hours after thrombolysis, recombinant human tissue plasminogen activator(rt-PA,10 mg/kg) was given to rats of the thrombolysis group through tail vein. Acupuncture was applied at “Shuigou”(CV26) and bilateral “Neiguan”(PC6) 2 h after successful modeling, and the needles were retained for 30 minutes. Cerebral blood flow(CBF) was monitored during modeling in each group, and the neurological deficit score(0-7 points) was given 2 h and 24 h after successful modeling according to Bederson's methods. The cerebral infarction volume was observed after triphenyltetrazole chloride(TTC) staining. The protein and mRNA expression levels of VEGF, bFGF and ES in the ischemic penumbra region of the right cerebral cortex were detected by Western blot and real-time PCR, separately. Results The neurological deficit score at both 2 h and 24 h after modeling, percentage of cerebral infarction volume, and the expression levels of VEGF, bFGF and ES proteins and mRNAs in the model group were significantly higher than those of the sham operation group(P<0.01, P < 0.05). Compared with the model group, the neurological deficit score 24 h(not at 2 h) after modeling and percentage of cerebral infarction volume, and the expression levels of ES protein and mRNA in the Acupunct+medication group(not in the medication group) were notably lower(P<0.05, P<0.01), while the expression levels of VEGF and bFGF proteins and mRNAs in the Acupunct +medication group(not in the medication group) were considerably higher(P<0.01, P<0.05). No significant differences were found between medication and model groups in the CI percentage, VEGF, bFGF and ES proteins and mRNAs(P>0.05). The therapeutic effect of Acupunct +medication group was significantly superior to that of medication in lowering neurological deficit score, percentage of CI volume and expression of ES protein and mRNA and in up-regulating the expression of VEGF and bFGF proteins and mRNAs(P<0.05, P<0.01). Conclusion Acupuncture and timely intervention can prolong the time window of thrombolysis in CI rats, which may be related to its function in up-regulating the expression of VEGF and bFGF mRNAs and proteins and in down-regulating the expression of ES mRNA and protein in ischemic cerebral cortex.关键词：Cerebral infarction;Acupuncture;Thrombolysis time window;Angiogenesis23|506|3更新时间：2023-08-11
- 摘要：Objective To observe the therapeutic effect of moxa fume in the treatment of chronic rhinosinusitis(CRS) and the effect of acupuncture plus smokeless moxibustion or smoky moxibustion on the expression of thymic stromal lymphopoietin(TSLP) and pituitary adenylate cyclase activating polypeptide(PACAP) proteins in the sinus mucosal tissue in CRS mice.Me-thods Sixty male C57 BL/6 J mice were randomly divided into 6 groups, namely normal control, sham operation, CRS model, medication, acupuncture plus smokeless moxibustion(Acu+smokeless Moxi) and acupuncture plus smoky moxibustion(Acu+smoky Moxi) groups, with 20 mice in each group. The CRS model was established by inserting a piece of polyporous sponge filled with streptococcus pneumoniae into the maxillary sinus after operation. The mice in the sham operation group received skin incision after opening the maxillary sinus. Mice of the medication group received gavage of clarithromycin 0.103 g·kg(-1)·d(-1)·d(-1) for 21 days. For mice of the Acu+smokeless Moxi and Acu +smoky Moxi groups, manual acupuncture stimulation was applied to bilateral “Zusanli”(ST36), “Shenshu”(BL23) and “Hegu”(LI4) with the needles retained for 30 min, once every other day, and on the following day, moxibustion was applied to “Guanyuan”(CV4) and “Shenque”(BL23) for 20 min, once every other day. The treatment was given for 21 days. Mice of the normal, sham operation and model groups received gavage of normal saline(200 μL/d) for 21 days. Histopathological changes of the nasal mucosa were observed after H.E. staining, the TSLP and PACAP contents and expression were determined by enzyme-linked immunosorbent assay(ELISA) and immunohistochemistry, separately.Results At the end of the treatment, mice of the model group still had symptoms of nasal obstruction and runny nose, but those of the 3 treatment groups were obviously relieved in the nasal symptoms. H.E. staining showed an obvious chronic inflammatory reaction in the sinus mucosa, uneven distribution of the mucosal epithelium and necrotic and exfoliated epithelial cells, hyperplasia of fibrous tissue in the submucosa, etc. in the model group, which were relatively milder in the medication, Acu+smokeless Moxi and Acu+smoky Moxi groups, while no obvious inflammation was found in the normal group and sham operation group. In comparison with the normal group, no significant changes were found in the expression levels of PACAP and TSLP in the sham operation group(P>0.05). The expression level of PACAP was significantly lower(P<0.05) and that of TSLP significantly higher in the model group than in the normal and sham operatin groups(P<0.05). Compared with the model group, no significant changes were found in the expression of PACAP in the medication, Acu+smokeless Moxi and Acu+smoky Moxi groups(P>0.05), and the expression of TSLP was further obviously increased in the Acu+smokeless Moxi group(P<0.01), but obviously decreased in the Acu+smoky Moxi group(P<0.01).Conclusion Acupuncture combined with smoky moxibustion can down-regulate the expression of TSLP protein in the nasal sinus mucosa in CRS mice, which maybe contribute to its effect in reducing the inflammatory reaction and nasal symptoms.关键词：Chronic rhinosinusitis;Acupuncture plus moxibustion;Pituitary adenylate cyclase activating polypeptide;Thymic stromal lymphopoietin26|464|0更新时间：2023-08-11
- 摘要：Objective To compare the effect of electroacupuncture(EA), metformin and EA plus metformin on the cognitive ability and senile plaques(SPs) in cerebral cortex and hippocampus of Alzheimer's disease(AD) mice, so as to explore a better treatment method for AD. Methods Twenty-four male APP/PS1 mice were randomly divided into model, metformin(medication), EA and EA+medication groups, with 6 mice in each group. Other 6 male wild C57 mice were used as the control group. EA(2 Hz, 1.0 mA) was applied to “Baihui”(GV20) and “Shenshu”(BL23) for 15 min, once a day, for 4 weeks, with 1 day's off every week. The mice of the medication group received gavage of metformin(300 mg·kg(-1)·d(-1)·d(-1)) once a day for 4 weeks. Morris water maze tests were used to assess the cognitive function of mice. H.E. staining was used to observe the histopathological changes of neurons in the cortex and hippocampus. Immunohistochemical method was used to observe the cerebral cortex and hippocampal SPs. The expression levels of SPs formation-related proteins: β-site amyloid precursor protein cleaving enzyme 1(βACE1) and insulin-degrading enzyme(IDE) in the cortex and hippocampus were detected by Western blot.Results Compared with the control group, the escape latency, number of SPs and the expression of βACE1 in the cortex and hippocampus were ob-viously increased(P<0.01), and the times of platform quadrant crossing and the expression of IDE protein were markedly decreased in the model group(P<0.01). In comparison with the model group, the escape latency, and the number of SPs and expression of βACE1 proteins in the cortex and hippocampus in the 3 treatment groups were significantly down-regulated(P<0.01), while the times of platform quadrant crossing, and the expression of IDE protein in both cortex and hippocampus of the three treatment groups were considerably up-regulated(P<0.01). Comparison among the three treatment groups showed that the therapeutic effect of EA+medication was significantly superior to that of medication and simple EA in down-regulating the escape latency, the number of SPs and expression of βACE1 in the cortex and hippocampus(P<0.01), and in up-regulating the times of the platform quadrant crossing, and expression of IDE protein in both cortex and hippocampus(P<0.01). No significant differences were found between the simple medication and simple EA in all the indexes mentioned above(P>0.05).Conclusion EA, metformin and EA plus metformin can improve cognitive ability and relieve SP formation in cerebral cortex and hippocampus in AD mice, which may be associated with their functions in down-regulating the expression of βACE1 and up-regulating the expression of IDE. The therapeutic effects of EA plus metformin are apparently better than those of simple EA and simple metformin.关键词：Alzheimer's disease;Electroacupuncture;Metformin;Senile plaques;Cerebral cortex;Hippocampus;Cognitive ability28|489|2更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion at myofascial trigger points on microglia activation and the expression of brain-derived neurotrophic factor(BDNF) in the spinal cord of rats with myofascial pain syndrome(MPS), so as to explore the central mechanism of the analgesic effect of moxibustion. Methods Twenty-four male SD rats were randomly divi-ded into control, model and moxibustion groups(n=8 in each group). The MPS model was established by strinking on fascia musculares and eccentric exercise. Rats in the moxibustion group were treated with mild moxibustion at myofascial trigger point for 15 min, once daily for 7 days. The thermal withdrawal latency(TWL) of rats was measured with a hot stabbing instrument. The pathological changes of the rat medial femoris muscle were observed after H.E. staining. The expressions of microglia marker(OX-42) and BDNF in the spinal dorsal horn were detected by immunohistochemistry and Western blot, separately. Results After modeling, the TWL of both the model and the moxibustion groups were significantly down-regulated(P<0.01),and were significantly decreased in contrast to that of the control group(P<0.01). After treatment and compared with the model group, the TWL of the moxibustion group was significantly increased(P<0.01) in the moxibustion group. Rat's muscle fibers of the control group were uniform in thickness and arranged tightly and regularly. While in the model group, some fractures and connective structure tissue renewal, irregular arrangement, and inflammatory cell infiltration were seen. The morphology of muscle fibers in the moxibustion group was close to normal, and the arrangement was neat and orderly, with a small amount of inflammatory cell infiltration. Compared with the control group, the expression of OX-42 and BDNF in the spinal dorsal horn of rats in the model group was increased(P<0.01). Following the treatment, and in comparison with the model group, the expression of OX-42 and BDNF of moxibustion group was down-regulated(P<0.05). Conclusion Moxibustion can significantly improve the injury of the medial femoral muscle and the TWL in MPS rats, which may be related to its effects in inhibiting the activation of spinal dorsal horn microglia and reducing the expression of BDNF.关键词：Myofascial pain syndrome;Moxibustion;Myofascial trigger points;Microglia;Brain-derived neurotrophic factor23|538|0更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the expression of NF-κB p65 in hippocampus and the morphology of hippocampus in rats with chronic fatigue syndrome(CFS), so as to explore its mechanism in improving cognitive dysfunction of CFS. Methods Forty-eight SD rats were randomly divided into control, model, EA and inhibitor groups(n=12 in each group). The CFS model was established by multi-factor compound stress stimulation method. Rats of the EA group received EA(50 Hz, 1 mA) at “Baihui”(GV20), Emotional Area I and bilateral Sensory Area for 30 min, once daily for 15 days. For rats in the inhibitor group, pyrrolidine dithiocarbamate(100 mg·kg(-1)·d(-1)·d(-1)) was injected intraperitoneally, once a day for 15 days. Learning and memory ability was evaluated by Morris water maze test. HE staining was used to observe the morphology of hippocampus. Western blot was used to determine the expression level of NF-κB p65 in hippocampus. Results After mode-ling, the general status score was increased(P<0.01), the escape latency was prolonged(P<0.01), the times of crossing the platform was decreased(P<0.01), and the expression level of NF-κB p65 in hippocampus tissue was significantly increased(P<0.05) in the model group compared with the control group. Compared with the model group, the general status score was decreased(P<0.01), the escape latency was shortened(P<0.01), the times of crossing the platform was increased(P<0.01), and the expression level of NF-κB p65 in hippocampus tissue was significantly decreased(P<0.05) in the EA and inhibitor groups. HE staining showed that in the model group, the hippocampal nerve cells were arranged disorderly, the structure was loose, and the number of apoptotic bodies and inflammatory cells was significantly increased. The degree of tissue damage of the EA and inhibitor groups was milder than that of the model group. Conclusion EA can improve the cognitive function in CFS rats, which may be associated with its effect in inhibiting the expression of NF-κB and reducing the inflammation response in hippocampus.关键词：Electroacupuncture;NF-κB;Chronic fatigue syndrome;Experimental study22|864|8更新时间：2023-08-11
- 摘要：Objective To explore the molecular mechanism of locus coeruleus(LC) involved in electroacupuncture(EA) anti myocardial ischemia. Methods Twenty-four SD rats were randomly divided into sham-operation, model, EA and EA +lesion groups, with 6 rats in each group. The acute myocardial ischemia(AMI) model was established by ligation of the left anterior descending branch of coronary artery. EA(2 Hz/15 Hz, 1 mA) was applied to bilateral “Shenmen”(HT7)-“Tongli”(HT5) and the middle-point between HT7 and HT5 for 30 min, once daily for 3 days. For rats of the EA +lesion group, the virus(300 nL) was injected into bilateral LC before EA treatment. Serum aspartate aminotransferase(AST) was detected by ELISA. The gene expression profiles of rat heart were detected by transcriptome sequencing, the differentially expressed genes were screened, and Gene Ontology(GO) functional classification and Kyoto Encyclopedia of genes and genomes(KEGG) metabolic pathway enrichment analysis were performed. Results Compared with the sham-operation group, serum AST content was significantly increased in the model group(P<0.01). Following the intervention, serum AST was significantly reduced in the EA group(P<0.01), while the serum AST in the EA + lesion group was significantly higher compared with the EA group(P<0.05). Differential expression analysis showed that 1 138 differentially expressed genes were screened out between the model group and the sham-operation group, 1 330 differentially expressed genes between model and EA group, and 804 differentially expressed genes between EA and EA + lesion group. Among them, 218 differential genes were involved in the regulation of EA anti-myocardial ischemia in LC. GO functional classification analysis showed that these differentially expressed genes mainly involved in cell processes, metabolic processes and biological regulation in biological processes. KEGG pathway analysis showed that these differentially expressed genes were enriched in sulfur relay system, thiamine metabolism, glutathione metabolism, C5 branch dicarboxylic acid metabolism, cell adhesion molecules and Th1 and Th2 cell differentiation. Conclusion EA intervention has a positive effect in anti-myocardial ischemia, which may be related to the sulfur relay system, thiamine metabolism, glutathione metabolism, C5 branch dicarboxylic acid metabolism, cell adhesion molecules and Th1 and Th2 cell differentiation involved in LC.关键词：Transcriptome;Acute myocardial ischemia;myocardial tissue;Electroacupuncture27|485|4更新时间：2023-08-11
- 摘要：Objective To observe the therapeutic effect of moxibustion combined with Guifu Yuhe decoction on allergic acne and the influence on immunologic function in the patients. Methods A total of 60 patients with allergic acne were rando-mized into an observation group(30 cases) and a control group(30 cases).Thirty healthy employees were in the healthy group. In the control group, Guifu Yuhe decoction was prescribed for oral administration, while in the observation group, on the base of the treatment as the control group, moxibustion was exerted at Dazhui(GV14) and Shenque(GV8). The treatment duration was 8 weeks. Before and after treatment, serum IgE, blood EOS, CD4+T cell, CD8+T cell, CD8+ T cell and CD4+ T cell and CD4+T/CD8+T/CD8+T, as well as the conversion score of idiopathic constitution and the symptom score were compared in the patients of two groups. The clinical therapeutic effect was observed in the two groups.Results Before treatment, compared with the healthy group, IgE and EOS, CD4+T, as well as the conversion score of idiopathic constitution and the symptom score were compared in the patients of two groups. The clinical therapeutic effect was observed in the two groups.Results Before treatment, compared with the healthy group, IgE and EOS, CD4+T cell and CD8+T cell and CD8+T cell all increased(P<0.05), and CD4+T cell all increased(P<0.05), and CD4+T/CD8+T/CD8+T decreased in the two groups(P<0.05). After treatment, IgE, EOS and CD4+T decreased in the two groups(P<0.05). After treatment, IgE, EOS and CD4+T cell and CD8+T cell and CD8+T cell decreased(P<0.05), and CD4+T cell decreased(P<0.05), and CD4+T/CD8+T/CD8+T increased(P<0.05) in the intra-group comparison in the patients. The changes of IgE, EOS, CD4+T increased(P<0.05) in the intra-group comparison in the patients. The changes of IgE, EOS, CD4+T cell and CD8+T cell and CD8+T cell in the observation group were more larger than those in the control group(P<0.05). After treatment, the conversion score of body constitution and symptom score all decreased in either group(P<0.05) and the scores in the observation group were lower than those of the control group(P< 0.05). The total effective rate of the observation group(29/30, 96.7%) was higher than that of the control group(22/30, 73.3%, P<0.05). Conclusion Moxibustion combined with Guifu Yuhe decoction can significantly improve immune function and body constitution of the patients with allergic acne, which may be related to rectifying idiopathic constitution, improving in lymphocyte subsets dysfunction and inhibiting allergic reaction.关键词：Allergic acne;Moxibustion;Idiopathic constitution;Immunological function19|315|0更新时间：2023-08-11
- 摘要：Objective To observe the clinical effect of moxibustion therapy based on “sancai yizhi”(benefiting the intelligence) therapy on the improvements of memory function and serum protein markers, Aβ1-42, Tau and phosphorylated Tau(P-tau) in the patients with amnestic mild cognitive impairment(aMCI), and has a preliminary exploration on its peripheral mechanism. Methods A total of 120 patients with aMCI were divided into a moxibustion group and a medication group using a random number table, 60 patients in each group. In the moxibustion group, 6 cases were dropped out and 5 cases were withdrawn, and then 49 cases accomplished the trial finally. In the medication group, 8 cases were dropped out and 6 ceases were withdrawn, thus 46 cases finally accomplished the trial. In the moxibustion group, moxibustion therapy was provided at Baihui(GV20), Shenque(CV8) and bilateral Yongquan(KI1), once every other day, 20 minutes each time, totally for 8 weeks. In the medication group, donepezil hydrochloride tablets were administered orally, 5 mg once a day, consecutively for 8 weeks. The scores of Rivermead behavioral memory test(RBMT) and Monterey cognitive assessment(MoCA) scale were adopted as the indicators to evaluate the therapeutic effect after treatment in the two groups. Enzyme linked immunosorbent assay(ELISA) was used to detect the changes of the levels of serum protein marker levels, i.e. Aβ1-42, Tau and P-tau before and after treatment in the patients of two groups. Results Compared with the scores before treatment, RBMT score and MoCA score all increased after treatment in the patients of two groups(P<0.05). Compared with the medication group at the same time points, RBMT score increased significantly(P<0.05) in the moxibustion group after treatment. In the moxibustion group, as compared with the levels before treatment, the levels of serum Aβ1-42,Tau and P-tau decreased after treatment in the patients(P<0.05). But in the medication group, the levels of serum Aβ1-42 and P-tau were reduced(P<0.05). Compared with the medication group at the same time points, there were no significant differences in the changes of serum Aβ1-42,Tau and P-tau in the moxibustion group(P>0.05). Conclusion Moxibustion therapy based on “sancai yizhi” theory improves the cognitive function in the patients with aMCI and it affects the levels of serum Aβ1-42, Tau and P-tau, which may be the reason for the improvement of cognitive function in the patients with aMCI.关键词：Amnestic mild cognitive impairment;“Sancai yizhi” moxibustion;Donepezil hydrochloride;Neuropsycholo-gical scale;Aβ;Tau;Phosphorylated Tau22|602|4更新时间：2023-08-11
- 摘要：Transcutaneous electrical acupoint stimulation(TEAS) has the characteristics of simple operation, non-invasive, and high patient acceptability, and is widely used in clinical practice. This article summarized the effects of TEAS on analgesia, gastrointestinal tract regulation, circulation regulation, postoperative cognitive function improvement, immune function regulation, anti-inflammatory and anti-stress during the perioperative period. At the same time, this article analyzed the problems of the application of TEAS in the perioperative period, and aimed to promote its clinical application.关键词：Transcutaneous electrical acupoint stimulation;Perioperative period;Clinical application21|964|5更新时间：2023-08-11
- 摘要：Depression is one of the major public health problems affecting both individuals and society. Acupuncture the-rapy has a positive effect in relieving depression, which has been researched widely in recent years. In the present paper, we reviewed the development of experimental researches on the mechanisms of acupuncture underlying relief of depressive disorders from eight aspects: 1) transmission of neural information, 2) neuroendocrine, 3) neural plasticity, 4) changes of signal pathways in the brain, 5) changes of related inflammatory cytokines, 6) neuroimaging(the related cerebral regions), 7) changes of whole genome, and 8) changes of other related systems. These results may provide a useful reference for future researches.关键词：Acupuncture;Depression;Mechanism;Review33|1818|4更新时间：2023-08-11
- 摘要：The scalp acupuncture therapy, an important component of the microneedle system of the acupuncturology, is effective in the treatment of multiple types of diseases. In the present paper, we made a comparative analysis about the theoretical basis, acupoint location and main clinical indications of the ten academic schools of scalp-acupuncture, including FANG Yun-peng's Scalp Acupuncture, JIAO Shun-fa's Scalp Acupuncture, ZHU Ming-qing's Scalp Acupuncture, etc. which are widely used in clinic at present after collecting the related articles published in recent 60 years from databases of CNKI, Wanfang and VIP, and relevant books. These nine academic schools are similar in clinical indications(such as neurological disorders, brain-derived di-seases) and in needle inserting angle and depth, and different in the needling manipulations, needling methods, needle retaining time, theoretical basis, attending diseases of the same one stimulated region, and the position and yin or yang of holograms. The main problems facing the scalp acupuncture are non-uniform positioning method, non-uniform needling method, and non-uniform indications of the same scalp acupoint. Up to now, it remains unclear that which academic school is better in the therapeutic efficacy, thus, we should strengthen clinical research to find out its inherent law in diagnosis and treatment, constantly optimize the needle scheme, determine the best scalp-acupoint position and best needling manipulations, quantize and standardize the related issues, and accelerate the integration of the different academic schools, so as to improve clinical curative effect and to further promote the application and popularization, serving the patients in a better way.关键词：Scalp acupuncture;Academic schools;Acupuncture method;Comparative study26|1590|9更新时间：2023-08-11
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