最新刊期
卷 47 ， 期 10 ， 2022
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the cardiac function, lymphatic markers, macrophage and inflammatory cytokines in acute myocardial ischemia(AMI) mice, so as to explore its mechanism in improving AMI. Methods Male C57 BL/6 mice were randomly divided into sham operation, model, EA, inhibitor and inhibitor+EA groups, with 10 mice in each group. AMI model was established by occlusion of left anterior descending coronary artery. For mice in the EA group and inhibitor+EA group, EA(1 mA, 2 Hz/15 Hz) was applied to bilateral “Shenmen”(HT7) and “Tongli”(HT5) for 30 min, once daily for consecutive 3 days. Mice in the inhibitor+EA group were given intraperitoneal injection of vascular endothelial growth factor receptor-3(VEGFR-3) inhibitor SAR131675 30 min before the EA, while mice in the inhibitor group were given intraperitoneal injection of SAR131675 only. The electrocardiogram(ECG) of the neck-thoracic lead was recorded and analyzed by BL-420 F biological function experiment system. Histopathologic changes of myocardial tissue were observed after H.E. staining. The contents of lactate dehydrogenase(LDH), cardiac troponin I(cTnI) in serum and interleukin-18(IL-18) and interleukin-6(IL-6) in ischemic myocardium were determined by ELISA. The expressions of hyaluronic acid receptor-1(LYVE-1) and macrophage mar-ker CD68(CD68) in the myocardial tissue were detected by immunofluorescence assay. The protein expression levels of vascular endothelial growth factor C(VEGF-C) and VEGFR-3 were detected by Western blot. Results Compared with the sham operation group, the ECG-ST level, the contents of serum LDH and cTnI, and the contents of IL-18 and IL-6 in the myocardial tissue were significantly increased(P<0.01), the expression of LYVE-1 and the protein expression levels of VEGF-C and VEGFR-3 in the myocardial tissue were significantly decreased(P<0.01), while the number of CD68 positive cells was significantly increased(P<0.01) in the model group. Compared with the model, inhibitor and inhibitor+EA groups, the ECG-ST level, the contents of serum LDH and cTnI, and the contents of IL-18 and IL-6 in the myocardial tissue were decreased(P<0.01), the expression of LYVE-1 and the expression level of VEGFR-3 protein were increased(P<0.01), while the number of CD68 positive cells was significantly decreased(P<0.01) in the EA group. Compared with the model and inhibitor groups, the expression level of VEGF-C was increased(P<0.01) in the EA group. Outcomes of H.E. staining showed that the myocardial fibers were disordered with a large number of inflammatory cell infiltration in the model group, which was milder in the EA group. Conclusion Acupuncture can improve the inflammatory injury of AMI mice, which may be related to activate VEGF-C/VEGFR-3 pathway to promote lymphangiogenesis, reduce macrophage infiltration and inflammatory factors.关键词：Acute myocardial ischemia;Electroacupuncture;Heart meridian;Vascular endothelial growth factor C/vascular endothelial growth factor receptor 3 pathway;Lymphangiogenesis;Inflammatory response31|481|1更新时间：2023-08-10
- 摘要：Objective To explore the effect of eye acupuncture on autophagy and expressions of autophagy-related proteins Beclin1, LC3 B,ATF6 and XBP1 in the infarction area of brain tissue in rats with acute cerebral ischemia-reperfusion injury(CIRI),so as to explore its mechanisms underlying improvement of CIRI. Methods Male SD rats were randomly divided into sham operation, model and eye acupuncture groups(n=16 in each group). The CIRI model was prepared by occlusion of the middle cerebral artery. Eye acupuncture stimulation was applied to bilateral “Gan”(Liver),“Shangjiao”(Upper-energizer),“Xiajiao”(Lower-energizer) and “Shen”(Kidney) regions at 0,12 and 24 h after CIRI,30 min each time. The neurological deficit score was given by referring to Longa's method, and TTC staining used to determine the success of model replication. After the treatment, the pathological changes of the cerebral infarction area were observed under light microscope, and the autophagosomes were observed by electron microscope. The protein expression levels of LC3 B,Beclin1,ATF6 and XBP1 in the infarction area of brain tissue were detected by Western blot. The immunoactivity of Beclin1 and the immunofluorescence density of ATF6 and XBP1 in the infarction area of brain tissue were determined by immunohistochemistry. Results The Longa's score, and the protein expression levels of LC3 B,Beclin1,ATF6 and XBP1 and immunoactivity or immunofluorescence density of Beclin1,ATF6 and XBP1 were significantly higher in the model group than those in the sham operation group(P<0.01),and considerably lower in the eye acupuncture group than those in the model group(P<0.01). Under light microscope, the model group had typical ethmoidal reticular cerebral infarction, while the eye acupuncture group had significantly smaller areas and clearer edges. Under electron microscope, there were more autophagosomes in the cytoplasm of neurons in the model group, and fewer autophagosomes in the eye acupuncture group(in contrast to the model group). Conclusion Eye acupuncture can improve the neurological function and mitigate cerebral injury in CIRI rats which may be associated with its function in inhibiting autophagy in the brain tissue by regulating ATF6 pathway.关键词：Eye acupuncture;Cerebral ischemia reperfusion injury;Endoplasmic reticulum stress;ATF6 pathway;Autophagy30|479|3更新时间：2023-08-10
- 摘要：Objective To investigate the mechanism of electroacupuncture(EA) “Zusanli”(ST36) in delaying colon “inflammation-cancer transformation” in mice by anti-inflammatory. Methods C57 BL/6 J mice were randomly divided into normal, model and EA groups, with 12 mice in each group. The mouse model of colorectal cancer(CRC) was established by intrape-ritoneal injection of azomethane(AOM) and feeding dextran sodium sulfate(DSS). At the beginning of the 2(nd) cycle, EA was applied to bilateral ST36 for 30 min once every other day for 12 times. The number of colon tumors in each group was observed, and the weight and length of colon were recorded. The contents of interleukin(IL)-1β, IL-6, IL-17 A,IL-23, tumor necrosis factor(TNF)-α and CXC chemokine ligand 1(CXCL1) of serum and colon tissue were detected by MSD multifactorial assay.The apoptosis of local cells in colon tumor was observed by TUNEL staining. Cell proliferation in colon tumor was observed by immunohistochemistry. Results Compared with the normal group, the colon length was significantly shortened(P<0.05) and the colon mass was significantly increased(P<0.001) in the model group, the contents of IL-1β, IL-6, TNF-α, IL-17 A and CXCL1 of serum and colon tissue were significantly increased(P<0.05,P<0.01,P<0.001), and the content of IL-23 was increased in colon tissue(P<0.05) in the model group. Compared with the model group, the colon mass was decreased(P<0.05) and the contents of IL-1β, IL-6, TNF-α and IL-17 A in serum were decreased(P<0.05), while the contents of IL-17 A, CXCL1 and IL-23 in colon tissue were decreased(P<0.05) in the EA group, the percentage of local apoptotic cells in the EA group was increased(P<0.001),the percentage of PCNA positive cells was decreased(P<0.001), the number of tumors and the tumor volume were significantly decreased(P<0.01,P<0.05). The contents of IL-6, TNF-α, IL-17 A and IL-23 in serum of CRC mice were positively correlated with tumor burden(P<0.05).The contents of IL-1β, TNF-α, CXCL1 and IL-23 in colon tissue of CRC mice were positively correlated with tumor burden(P<0.05).Conclusion Electroacupuncture at ST36 can inhibit the inflammatory response of AOM/DSS inflammatory associated CRC mice and delay the “inflammation-cancer transformation” of colon.关键词：Colorectal cancer;Electroacupuncture;Inflammatory reaction;Inflammatory-cancer transformation36|526|4更新时间：2023-08-10
- 摘要：Objective To observe the effect of “nape seven needles” on the expressions of hypoxia inducible factor-1α(HIF-1α) and Notch1 in cervical intervertebral disc of cervical disc degeneration(CDD) rats, so as to reveal its underlying mecha-nisms in improving CDD. Methods SD male rats were randomly divided into normal, model, non-acupoint and nape seven needles groups, with 10 rats in each group. Staticdynamic imbalance method was used to establish CDD model. Rats in the nape se-ven needles group were treated with acupuncture at “Fengfu”(GV16), and bilateral “Fengchi”(GB20), “Wangu”(GB12) and “Tianzhu”(BL10), and rats in the non-acupoint group received acupuncture at the sham acupoints at the caudal tip and armpit, both for 20 min, 6 days a week for 4 weeks. After intervention, tilted plane test and spiral CT were used to assess the neck movement function and cervical degeneration degree of rats; immunohistochemistry was used to observe the protein expression levels of HIF-1α and Notch1 in cervical discs tissue; Western blot and real-time quantitative PCR were used to detect the protein and mRNA expression levels of HIF-1α and Notch1 in cervical discs tissue, respectively. Results After modeling, the cervical curvature was straightened, with narrowed intervertebral space, rough and hardened articular surface, osteophytes, and blurred articular space and articular process, which was relatively milder in the nape seven needles group. Compared with the normal group, the angle of tilted plane was significantly reduced(P<0.05), while cervical scores, HIF-1α mRNA expression level in cervical intervertebral disc tissue were significantly increased(P<0.05) in the model group. Compared with the model and the non-acupoint groups, cervical scores were significantly reduced(P<0.05), while the angle of tilted plane, HIF-1α and Notch1 positive expressions, HIF-1α mRNA expression level, Notch1 protein and mRNA expression levels in cervical intervertebral disc tissue were significantly increased(P<0.05) in the nape seven needles group. Conclusion Acupuncture of “nape seven needles” can reduce the degree of cervical degeneration in rats, which was possibly associated with its effects in up-regulating the expressions of HIF-1α and Notch1 in cervical intervertebral disc tissue, promoting the proliferation and recovery of endogenous cells in nucleus pulposus.关键词：Nape seven needles;Cervical disc degeneration;Hypoxia inducible factor-1α;Notch117|282|0更新时间：2023-08-10
- 摘要：Objective To observe the effect of herbal cake-separated moxibustion(HCSM) on serum lactic acid(BLA) level and AMPK/PGC-1α signaling pathway in the quadriceps femoris in chronic fatigue syndrome(CFS) rats, so as to explore its mechanisms underlying improvement of CFS. Methods According to the random number table, 50 SD rats were divided into blank control, model, HCSM, sham HCSM and medication(herbal medicine gavage) groups, with 10 rats in each group. The CFS model was established by using chronic restraint and exhaustive swimming, alternately, once daily for 21 days. The herbal cake was made of Xiaoyao Powder(Mental Ease Powder, composed of [Danggui(Radix Angelicae Sinensis), Baishao(Radix Paeoniae Alba), Chaihu(Radix Bupleuri), Fuling(Poria), Baizhu(Rhizoma Atractylodis, Macrocephalae), etc.]. The HCSM was applied to “Shenque”(CV8), “Guanyuan “(CV4), bilateral “Zusanli”(ST36) and “Qimen”(LR14), 5 moxa-cones for each acupoint, once daily for 10 days. For sham HCSM, the excipient was instead of herbal cake, and the same 5 moxa-cones was given as the HCSM group. Rats of the medication group received gavage of Xiaoyao Powder suspension(60 mg·kg(-1)), once daily for 10 days. The open field test and tail suspension test were conducted for determining the animals' locomotor activity. The blood sample was taken from the abdominal aorta under anesthesia for assaying the levels of serum BLA, chemokine ligand CXCL9 and β-endorphin(EP) by ELISA. Bilateral quadriceps femoris were sampled for observing histopathological changes after staining with conventional H.E. technique, and for detecting the expression levels of phosphorylated AMP-activated protein kinase(p-AMPK) and peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α) by using immunohistochemistry. ResultsCompared with the blank control group, the number of rearing and horizontal grid-crossing times, struggling times of tail suspension test were significantly decreased(P<0.05), and the immobility time was obviously prolonged(P<0.05) in the model group. Compared with the model group, both HCSM and medication groups had a significant increase of rearing, horizontal grid-crossing times and struggling times(P<0.05), and the immobility time had a significant decrease(P<0.05). But there were no significant differences in the total movement distance among the 5 groups(P>0.05), and in the 5 indexes of behavioral measurements between the HCSM and medication groups(P>0.05). The sham HCSM could also evidently increase the struggling times and reduce the immobility time(P<0.05). The contents of serum BLA, CXCL9 and β-EP were obviously higher in the model group than in the blank control group(P<0.05), as well as remarkably lower in the HCSM and medication groups than in the model group(P<0.05). Whereas the expression levels of muscular p-AMPK and PGC-1α were considerably lower in the model group than in the blank control group(P<0.05), and significantly increased in both HCSM and medication groups relevant to the model group(P<0.05). Compared with the sham HCSM group, the contents of BLA, CXCL9 and β-EP in serum of the HCSM group and contents of CXCL9,β-EP in medication group were significantly decreased(P<0.05), and the protein expressions of p-AMPK and PGC-1α in quadriceps femoris in both HCSM and medication groups were significantly increased(P<0.05). H.E. staining showed smaller intercellular space, uneven cytoplasmic staining in some muscle fibers, nucleus pyknosis and condensation, and inflammatory cell infiltration in the model group, which was milder in both HCSM and medication groups. Conclusion HCSM can mitigate the stress behavioral state in CFS rats, which may be related with its functions in lowering the levels of serum BLA, CXCL9 and β-EP, and activating AMPK/PGC-1α signaling pathway(balancing energy metabolism) in the quadriceps femoris.关键词：Herbal cake-separated moxibustion;Chronic fatigue syndrome;Energy metabolism;Lactic acid;AMPK/PGC-1α signaling pathway33|595|4更新时间：2023-08-10
- 摘要：Objective To observe the effect of electroacupuncture(EA) on blood-brain barrier(BBB) permeability and proinflammatory cytokines interleukin-1β(IL-1β) and interleukin-18(IL-18) in the hippocampus of vascular dementia(VD) rats, so as to explore the mechanism of EA on treatment of VD. Methods SD male rats were randomly divided into sham operation, model, and EA groups, with 15 rats in each group. The VD rat model was established by permanently occlusion of the bilateral middle cerebral artery. Rats of the EA group received EA at “Baihui”(GV20),“Dazhui”(GV14), and bilateral “Shenshu”(BL23) for 30 min, 6 days a week for a total of 4 weeks. Morris water maze test was used to assess the cognitive function of rats. Evans blue staining was used to detect the BBB permeability, transmission electron microscopy and ELISA were used to detect the ultrastructure of BBB and the contents of hippocampal IL-1β and IL-18, respectively. Results Following modeling, compared with the sham operation group, the mean escape latency of model group was significantly prolonged(P<0.01), the times of crossing the platform were significantly decreased(P<0.01), the content of Evans blue, and the contents of IL-1β and IL-18 in hippocampus were increased(P<0.01). After the intervention, comparison between the model and EA groups showed that the average escape latency of rats in EA group was significantly shortened(P<0.01), the times of crossing the platform were increased(P<0.05), the content of Evans blue, and the contents of IL-1β and IL-18 in hippocampus were significantly decreased(P<0.01). The ultrastructure of BBB was moderately damaged in the model group, which was evidenced by blurred endothelial cell membrane structure, obviously dropsical astrocyte foot process, and decreased tight junctions. The ultrastructure of BBB was slightly damaged and astrocyte foot had no obvious edema in the EA group. Conclusion EA can significantly improve the learning and memory ability of VD rats and improve the BBB permeability, which may be related to its effect in inhibiting the expression of IL-1β and IL-18 in the hippocampus.关键词：Electroacupuncture;Vascular dementia;Blood-brain barrier;Interleukin-1β;Interleukin-1834|666|10更新时间：2023-08-10
- 摘要：Objective To observe the effect of acupuncture at “Sanyinjiao”(SP6) on sperm motility, testicular B cell lymphoma/leukelia-2(Bcl-2), Bcl-2 associated X protein(Bax), and Caspase-3 in mice with oligoasthenospermia induced by microwave radiation, so as to explore its underlying mechanisms in improving oligoasthenospermia. Methods Male BALB/C mice were randomly divided into control, model and acupuncture groups(n=6 in each group). The oligoasthenospermia model was established by continuous microwave irradiation with frequency of 2 450 MHz and power density of 40 mW/cm2, 1 h daily for 18 days. At the same time, manual acupuncture was applied to the acupuncture group on bilateral “Sanyinjiao”(SP6) for 30 s, once daily for 18 days. Sperm motility including the percentages of progressive motility(PR), non-progressive motility(NP), and PR + NP sperms was detected by computer-assisted sperm analysis, H.E. staining was used to observe the testicular morphology and Johnson score was calculated, the expression levels of Bcl-2, Bax and Caspase-3 in testis were detected by immunofluorescence. Results Compared with the control group, the percentages of PR sperms, NP sperms, PR+NP sperms, Johnson score, and expression level of Bcl-2 were significantly decreased(P<0.05), while the expression levels of Bax and Caspase-3 were increased(P<0.05) in the model group. Compared with the model group, the percentages of PR sperms, PR+NP sperms, Johnson score, and expression level of Bcl-2 were significantly increased(P<0.05), while the expression levels of Bax and Caspase-3 were decreased(P<0.05) in the acupuncture group. Outcomes of H.E. staining showed that the seminiferous tubules became thinner, spermatogenic cells and sperm decreased or even disappeared, and the supporting cells were partially missing in the model group, which was relatively milder in the acupuncture group. Conclusion Manual acupuncture at SP6 can improve sperm motility in oligoasthenospermia mice induced by microwave radiation, which may be related to its effects in down-regulating the expressions of Bax and Caspase-3, increasing expression of Bcl-2 in the testis.关键词：Acupuncture;Sanyinjiao;Microwave radiation;Oligoasthenospermia;Sperm motility;Apoptosis28|400|4更新时间：2023-08-10
- 摘要：Objective To observe the effect of electroacupuncture on sexual development and ovarian estrogen receptor β(ER-β) expression in female adolescent obese rats induced by high-fat diet, so as to explore its underlying mechanisms of improving adolescent obesity. Methods Female SD rats(age of 21 days) were randomly divided into control, model and acupuncture groups, with 6 rats in each group. The obese model was established by feeding high-fat diet for 6 weeks. Rats of the acupuncture group received electroacupuncture(2 Hz, 0.5-1.2 mA)stimulation at bilateral “Sanyinjiao”(SP6),“Fenglong”(ST40) and “Zusanli”(ST36) for 30 min, once a day for 14 days. The body mass and abdominal circumference of rats were measured before and after treatment. The contents of serum follicle stimulating hormone(FSH), luteinizing hormone(LH) and estradiol(E_2) were detected by ELISA. The number of corpus luteum and follicle were observed by HE staining. The expression levels of ER-β mRNA and protein in ovary were detected by fluorescence quantitative PCR and Western blot, respectively. Results Compared with the control group, the body mass and abdominal circumference, the contents of serum FSH and E_2, and the expression levels of ER-β mRNA and protein in ovary were significantly increased(P<0.05)in the model group, while the number of mature follicles and corpus luteum increased significantly. Compared with the model group, the body mass and abdominal circumference, the contents of serum FSH and E_2, and the expression levels of ER-β mRNA and protein in ovary were significantly decreased(P<0.05) in the acupuncture group, while the number of mature follicles and corpus luteum decreased significantly.Conclusion Electroacupuncture can effectively improve the levels of sex hormone and the development of ovary, down-regulate the expression levels of ER-β mRNA and protein in ovary, so as to regulate the process of sexual development of female adolescent obese rats induced by high-fat diet.关键词：Electroacupuncture;High-fat diet;Female rat;Sexual development;Estrogen receptor β27|232|0更新时间：2023-08-10
- 摘要：Objective To observe the effects of point-pricking method with fire needle on the symptoms of knee joint and physio-psychological health in the patients with knee osteoarthritis(KOA). Methods Sixty six patients with KOA were randomly divided into a fire needling group(33 cases) and a filiform needling group(33 cases). The patients received the point-pricking method with fire needle in the fire needle group while the conventional acupuncture with filiform needle was provided in the filiform needling group. The basic health management was performed in both groups. The acupoints included bilateral Liangqiu(ST34), Xuehai(SP10), Dubi(ST35), Neixiyan(EX-LE4), Yanglingquan(GB34) and Zusanli(ST36) as well as Ashi points. The treatment was conducted twice a week for 6 weeks consecutively. Before and after treatment, the scores of Western Ontario and McMaster University Osteoarthritis Index(WOMAC), traditional Chinese medicine(TCM) symptoms and visual analogue score(VAS), the numbers of affected areas of knee joint pain and the scores of 12-item short-form health survey(SF-12) were assessed and the incidence of adverse effects was recorded. Results The score for pain, stiffness and function, as well as the total score of WOMAC were all reduced(P<0.05), the score of TCM symptoms, VAS score and the numbers of the affected areas of knee joint pain were lower(P<0.05) after treatment when compared with those before treatment within group. After treatment, the score of each domain of SF-12(i.e. general health, physical function, role-physical, vitality, role-emotional, physical component summary and mental component summary) was higher in comparison to those before treatment in both groups(P<0.05), while the scores of bodily pain, mental health and social functioning in SF-12 were increased in the fire needling group(P<0.05). Compared with the filiform needling group, the score of joint function and the total score in WOMAC and VAS score were reduced remarkably(P<0.05), and general health score in SF-12 was more obviously increased(P<0.05) in the fire needling group after treatment. None adverse effects were found in either group. Conclusion The point-pricking method with fire needle is safe and effective when compared with conventional acupuncture with filiform needle. In the aspects of improving knee joint function, relieving joint pain and advancing the quality of life, the point-pricking method with fire needle is superior to the conventional acupuncture with filiform needle.关键词：Knee osteoarthritis;Filiform needling;Fire needling;Efficacy evaluation;Safety59|719|3更新时间：2023-08-10
- 摘要：Objective To investigate the therapeutic effect of acupuncture stimulation combined with administration of“Gushen Zhuyu Tang”(decoction for consolidating kidney to eliminate blood stasis, DCKEBS) in the treatment of lumbar disc herniation(LDH) patients. Methods A total of 147 patients with LDH were randomly divided into DCKEBS, acupuncture and DCKEBS+acupuncture groups(n= 49 cases in each group). The patients of the acupuncture group received a) acupuncture stimulation of Dazhui(GV14), Ganshu(BL18), Shenshu(BL23), Tianshu(ST25), Yanglingquan(GB34),etc., b) fire needle pricking of the topical tendons, cord-like points, tender-points, c) row-needles stimulation of the attachment sites of muscles of the sacroiliac joint or crista iliaca, and d) acupotomy-debonding of the topical high-tension muscles, twice a week for 4 weeks. Those patients of the DCKEBS group were ordered to take DCKEBS [containing fried Yiyiren(Semen Coicis), Shanzhuyu(Fructus Corni), fried Baizhu(Rhizoma Astractylodis), Sangjisheng(Ramulus Loranthi), Duzhong(Cortex Eucommiae), Buguzhi(Fructus Psoraieae), etc.] 150 mL, twice daily, continuously for 4 weeks, and those of the DCKEBS+acupuncture group received the combined treatment mentioned above in the acupuncture and DCKEBS groups. The pain severity was assessed by using visual ana-logue scale(VAS, 0—10 points) and the modified Japan Orthopaedic Association questionnaire(M-JOA) score(0—30 points), separately, and the lumbar range of motion(ROM) and lumbar muscle strength were tested to evaluate the lumbar motor function. The levels of serum tumor necrosis factor α(TNF-α), matrix metalloproteinase-2(MMP-2), and apoptosis related factors Caspase-3 and Caspase-9 were assayed using ELISA. The total effective rates of the three groups were compared. Results After the treatment, the VAS and M-JOA scores, contents of serum TNF-α, MMP-2, Caspase-3 and Caspase-9 were significantly decreased(P<0.01), and the myodynamia of lumbar muscular flexor and extensor was considerably increased(P<0.01) in the three groups, and the ROM angles of lumbar extending and buckling were increased(P<0.01) in the DCKEBS+acupuncture group compared with pretreatment. Comparison among the 3 groups showed that the VAS and M-JOA scores, and serum TNF-α, MMP-2, Caspase-3 and Caspase-9 contents of the DCKEBS+acupuncture group were significantly lower than those of both DCKEBS and acupuncture groups(P<0.01), while the ROM angles of lumbar extending and buckling, and the myodynamia of lumbar muscular flexor and extensor were obviously higher in the DCKEBS+acupuncture group than those of the DCKEBS and acupuncture groups(P<0.01). The total effective rate was 93.88%(46/49) in the DCKEBS+acupuncture group, higher than 75.51%(37/49) in the DCKEBS group and 71.43%(35/49) in the acupuncture group(P<0.05). Conclusion Acupuncture combined with DCKEBS can relieve pain, improve lumbar muscle strength and lumbar movement function, and reduce serum TNF-α, MMP-2, Caspase-3 and Caspase-9 levels in LDH patients.关键词：Lumbar disc herniation;Acupuncture;Traditional Chinese medicine;Apoptosis71|574|1更新时间：2023-08-10
- 摘要：Objective To observe the clinical effect of acupotomy combined with warm needling on cervical spondylotic radiculopathy(CSR) of qi and blood stagnation syndrome. Methods A total of 90 CSR patients were randomly divided into an acupotomy group, a warm needling group and a combined treatment group, with 30 cases in each group. The patients in the acupotomy group were treated with acupotomy, once every 7 days, consecutively for 3 times. The patients in the warm needling group received warm needling, once daily, at the interval of 2 days after consecutive treatments for 5 days, 7 days as one session of treatment and 3 consecutive sessions were required. The patients in the combined treatment group were treated with acupotomy and warm needling, and the methods and the treatment session were same as the the previous two groups. Before and after the treatment, the pain rating index(PRI) of McGill pain questionnaire(MPQ) and the 20-point scale of CSR developed by Yasuhisa Tanaka(CSR20) were adopted in the assessment. The changes of clinical symptoms and functions of patients were observed and the clinical efficacy was assessed in each group. Results After the treatment, the PRI score was decreased(P<0.05) and the CSR20 score was increased(P<0.05) in the 3 treatment groups when compared with those before the treatment. After the treatment, compared with the acupotomy group and the warm needling group, the PRI score was decreased(P<0.05) and the CSR20 score was increased(P<0.05) in the combined treatment group. The total effective rate was 83.3%(25/30) in the acupotomy group, 76.7%(23/30) in the warm needling group and 93.3%(28/30) in the combined treatment group. The total effective rate in the combined treatment group was higher than those in the acupotomy group and the warm needling group(P<0.05). Conclusion The combined treatment with acupotomy and warm needling may obviously improve the clinical symptoms and physical signs, e.g. pain and numbness in the patients with CSR of qi and blood stagnation syndrome. Its efficacy is remarkably higher than that of the simple application of acupotomy or warm needling.关键词：Cervical spondylotic radiculopathy;Qi and blood stagnation syndrome;Acupotomy;Warm needling38|1056|1更新时间：2023-08-10
- 摘要：Objective To analyze the application characteristics of acupuncture and moxibustion in clinical treatment of Meniere's disease by using complex network technology, so as to provide evidence for selecting acupoints, needling and moxibustion methods and treatment ideas. Methods Articles both in English and Chinese published from the inception of databases of CNKI, Wanfang VIP, Chinese biomedical literature database(SinoMed), PubMed, Embase, EBSCO(Academic Search Pre-mier), Web of Science and Ovid to April of 2021 were retrieved by using key words “acupuncture” or “moxibustion” or “acupuncture and moxibustion” and “Meniere disease” or “Meniere's syndrome” or “Ménières vertigo” or “otogenic vertigo” or “auditory vertigo”, followed by screening the literature according to the inclusion and exclusion criteria and establishing a database of clinical li-terature about acupuncture treatment of Meniere's Disease with software Epidata 3.1. Then, the descriptive analysis was conducted first, followed by association rule analysis using SPSS Modeler 18.0, and complex network analysis using Gephi 0.9.2 software. Results A total of 232 articles were included, containing 152 acupoints [97 body acupoints as Baihui(GV20), Fengchi(GB20), Neiguan(PC6), etc.,28 otopoints as Ershenmen(MA-TF1), Shen(MA-SC), etc., 20 scalp points as Yunting Area, 7 extra-points as Sishencong(EX-HN1), Taiyang(EX-HN5), etc.] which were used to be a total frequency of 1 569. Descriptive analysis showed that the main meridians were the Governor Vessel, Stomach Meridian of Foot Yangming, Trienergizer Meridian of Hand Shaoyang, and Gallbladder Meridian of Foot Shaoyang. Acupuncture was the most commonly used therapy for Meniere's disease. The association analysis showed that the most relevant combination of acupoints was GV20 and GB20, GV20 and PC6, reflecting the principles of local acupoint selection and combination of local and distant acupoints. Finally, “K-core Analytic Hierarchy Process” and “Community Analysis” revealed that 3 core acupoint groups were most frequently used in clinical treatment of Meniere's disease, including 1) auricular acupoints, as MA-TF1, MA-SC,Neier(MA-L), Zhen(MA-AT) and Pizhixia(MA-AT1), 2) acupoints of the 14 meridians and extra-points, as Tinggong(SI19), Yifeng(TE17), GB20, 3) acupoints of the Shaoyang meri-dians of hand and foot, as Shuaigu(GB8), Tinghui(GB2), Zhongzhu(TE3), Ermen(TE21), etc. Conclusion The principle of acupoint selection is mainly based on the combination of acupoints along the meridians and local areas, while paying attention to the coordination among the auricular points, scalp acupoints and extra-points, which may provide a reference for the clinical treatment and scientific research on acupuncture treatment of Meniere's disease.关键词：Meniere's disease;Data mining;Complex network technology;Acupuncture and moxibustion;Clinical application characteristics35|994|3更新时间：2023-08-10
- 摘要：Objective To analyze and sum up clinical application regularities of Chengshan(BL57) acupoint in ancient Chinese literature. Methods The book Zhonghua Yidian(Collection of Chinese Medical Classic Works, 5(th) edition) containing 1 156 ancient medical books was used as the retrieved literature source. The key retrieval words used were BL57 acupoint related indications, prescriptions, treatment methods and taboos, followed by sorting out relevant articles and establishing a database.Results A total of 196 articles meeting our inclusion standards of the present study, involving 61 ancient books which contain 174 related terms covering 56 diseases or syndromes of internal medicine, surgery, pediatrics, ophthalmology and otorhinola-ryngology, etc.. Among them, the most frequently attending disease treated by single BL57 or acupoints combination was limb meridian disorders, and the most frequently used auxiliary acupoint was Kunlun(BL60). The most frequently used auxiliary meridian was the Bladder Meridian of Foot Taiyang. In addition, a total of 71 terms involved the acupuncture and moxibustion techniques of BL57, covering 7 treatment methods. In terms of the treatment methods, the dose of moxibustion was five cones in each session, and the depth of acupuncture needle insertion was about seven fen. Conclusion BL57 is widely used in various clinical departments, especially for limb meridian diseases.关键词：Chengshan(BL57);Ancient literature;Indications;Acupoint compatibility;Acupuncture needle insertion depth;Moxibustion21|605|0更新时间：2023-08-10
- 摘要：Acupuncture animal experiment is the main form of acupuncture mechanism research, in which, the setting of control group is crucial to the scientificity and reliability of research conclusion, and the research elements are the fundamental. Focusing on the research elements involved in acupuncture animal experiment, this study summarized the current situation and characteristics of the control groups related to acupoint selection, intervention and pathological mechanism in acupuncture animal experiment, and put forward the questions and suggestions so that the references of setting control group can be provided for acupuncture animal experiment in future.关键词：Acupuncture;Animal experiment;Control group;Sham acupuncture23|427|0更新时间：2023-08-10
- 摘要：Shu-Mu acupoint combination is commonly employed in acupuncture and moxibustion treatment. In animal experiments, it is difficult to implement such combination because of the uncontrollable features of animals. Therefore, there is an urgent need to design an experimental device to facilitate the operation of acupuncture and moxibustion at Shu-Mu acupoints in rats. The present study designed a device equipped with a fixed plate and a moxibustion frame for rats. The fixed plate and the moxibustion frame with detachable connection can allow acupuncture on the back, chest, and abdomen of rats to meet the requirements of the Shu-Mu acupoint combination. The moxibustion frame is made of universal hose and equipped with moxa clip, which ensures moxibustion in any direction and performs moxibustion on acupoints in the back, chest, and abdomen simultaneously. This device is unique in structure and convenient in operation, with safety and reliability. It is beneficial to the development of animal experiments related to acupuncture and moxibustion of Shu-Mu acupoint combination in the future.关键词：Fixation frame for rats;Acupuncture;Moxibustion;Combination of Shu-Mu acupoints18|307|0更新时间：2023-08-10
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