最新刊期
卷 47 ， 期 4 ， 2022
- 摘要：Objective To observe the effect of acupuncture at “Yanglingquan”(GB34) and “Baihui”(GV20) on Na+/K+/K+-ATPase, excitatory amino acid transporters(EAATs) and glutamate(Glu) in hippocampus of post-stroke spasticity rats, so as to explore the central mechanism in anti-spasticity. Methods In a total of 48 healthy SD rats, 12 rats were randomly selected to be included into sham operation group, and the remaining rats were used to make a middle cerebral artery occlusion(MCAO) model using a suture method. On the 3+-ATPase, excitatory amino acid transporters(EAATs) and glutamate(Glu) in hippocampus of post-stroke spasticity rats, so as to explore the central mechanism in anti-spasticity. Methods In a total of 48 healthy SD rats, 12 rats were randomly selected to be included into sham operation group, and the remaining rats were used to make a middle cerebral artery occlusion(MCAO) model using a suture method. On the 3(rd) day after modeling, MCAO limb spasticity rats were screened by neurological deficit symptoms and muscle tension scores, and randomly divided into the model, GB34(Hui-puncture at GB34) and GB34+GV20(Hui-puncture at GB34 and horizontal insertion at GV20) groups(n=12 rats in each group), and the treatment was lasted for 7 conse-cutive days. The neurological symptoms and muscle tension score were observed with the Zea Longa score and modified Ashworth scale(MAS). The levels of Glu, EAAT1(GLAST) and EAAT2(GLT-1) in the ischemic area of cerebral hippocampus were detected by ELISA, the expression of Na(rd) day after modeling, MCAO limb spasticity rats were screened by neurological deficit symptoms and muscle tension scores, and randomly divided into the model, GB34(Hui-puncture at GB34) and GB34+GV20(Hui-puncture at GB34 and horizontal insertion at GV20) groups(n=12 rats in each group), and the treatment was lasted for 7 conse-cutive days. The neurological symptoms and muscle tension score were observed with the Zea Longa score and modified Ashworth scale(MAS). The levels of Glu, EAAT1(GLAST) and EAAT2(GLT-1) in the ischemic area of cerebral hippocampus were detected by ELISA, the expression of Na+/K+/K+-ATPase α1(ATP1α1) was detected by Western blot, the expression of ATP1α1 mRNA was detected by real-time PCR, and the expression of GLAST, GLT-1 and ATP1α1 was detected by immunofluorescence. Results After modeling, Zea Longa score and MAS score were increased(P<0.01), the level of Glu in the ischemic area of cerebral hippocampus was increased(P<0.01), while the expression levels of GLAST, GLT-1, ATP1α1 protein and mRNA were all decreased(P<0.01) in the model group relevant to the sham operation group. After 7 days' treatment, all the increased and decreased levels of the indexes mentioned above were reversed in the two acupuncture groups relevant to the model group(P<0.01, P<0.05), and the effects of acupuncture at GB34+GV20 were obviously superior to that of acupuncture at GB34(P<0.05, P<0.01). Conclusion Acupuncture can alleviate post-stroke spasticity effectively, which may be related to its effect in up-regulating the expressions of Na+-ATPase α1(ATP1α1) was detected by Western blot, the expression of ATP1α1 mRNA was detected by real-time PCR, and the expression of GLAST, GLT-1 and ATP1α1 was detected by immunofluorescence. Results After modeling, Zea Longa score and MAS score were increased(P<0.01), the level of Glu in the ischemic area of cerebral hippocampus was increased(P<0.01), while the expression levels of GLAST, GLT-1, ATP1α1 protein and mRNA were all decreased(P<0.01) in the model group relevant to the sham operation group. After 7 days' treatment, all the increased and decreased levels of the indexes mentioned above were reversed in the two acupuncture groups relevant to the model group(P<0.01, P<0.05), and the effects of acupuncture at GB34+GV20 were obviously superior to that of acupuncture at GB34(P<0.05, P<0.01). Conclusion Acupuncture can alleviate post-stroke spasticity effectively, which may be related to its effect in up-regulating the expressions of Na+/K+/K+-ATPase and EAATs in hippocampus. The anti-spastic effect of acupuncture at GB34+GV20 is superior to GB34 alone.关键词：Post-stroke spasticity;Acupuncture;Glutamate;Na~+/K~+-ATPase;Excitatory amino acid transporters44|986|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of mild moxibustion on monocyte chemotaxis protein 1(MCP-1)/matrix metalloproteinase 2(MMP-2)/transforming growth factor β1(TGF-β1) pro-inflammatory signal loop in senile rats, so as to explore its mechanisms underlying improving vascular aging(VA). Methods Twenty-four male VA SD rats were randomized into senium(VA) control, medication and moxibustion groups, and other 8 young SD rats(aged 2 months) were used as the young control group. The VA model was established by intraperitoneal injection of D-galactose(300 mg·kg(-1)·d(-1)·d(-1)) once daily for 4 weeks, and verified by serum total testosterone(TT) and free testosterone(FT) levels. For rats of the moxibustion group, mild moxibustion was applied to bilateral “Shenshu”(BL23) and “Guanyuan”(CV4) for 20 min, once a day, 5 days a week for 8 weeks. Rats of the medication group were treated by intraperitoneal injection of testosterone propionate(7 mg·kg(-1)) once daily for 4 weeks, and verified by serum total testosterone(TT) and free testosterone(FT) levels. For rats of the moxibustion group, mild moxibustion was applied to bilateral “Shenshu”(BL23) and “Guanyuan”(CV4) for 20 min, once a day, 5 days a week for 8 weeks. Rats of the medication group were treated by intraperitoneal injection of testosterone propionate(7 mg·kg(-1)·[3 d](-1)·[3 d](-1)) once daily for 8 weeks except weekends, and rats of the senium control and young control groups treated by intraperitoneal injection of the same dose of normal saline, once daily for 8 weeks except weekends. The duration of exhausted swimming(DES) before and after the treatment was recorded. H.E. staining and Masson staining were used to observe histopathological changes and collagen fiber content of the thoracic aortic tissue, respectively. The contents of serum TT, FT and angiotensin 2(Ang Ⅱ) were determined by ELISA. The immunoactivity of aortic MCP-1 was detected by immunohistochemistry, and the expression levels of aortic MCP-1, MMP-2 and TGF-β1 were detected by Western blot. Results Compared with the young control group, the levels of DES, serum TT and FT contents were significantly decreased(P<0.01), while those of serum AngⅡ and collagen fiber contents, aortic MCP-1 immunoactivity and MMP-2, TGF-β1 and MCP-1 protein expression considerably increased in the senium control group(P<0.01). After the interventions, the decreased levels of DES, serum TT and FT contents and the increased levels of serum AngⅡ, collagen fiber contents, aortic MCP-1 immunoactivity and MMP-2, TGF-β1 and MCP-1 protein expression were reversed in both medication and moxibustion groups(P<0.01, P<0.05). The therapeutic effect of mild moxibustion was significantly superior to that of medication in down-regulating the aortic collagen fiber, serum AngⅡ contents and MCP-1 immunoactivity and protein expression(P<0.05). H.E. staining showed thickened endometrium and disordered arrangement of vascular smooth muscles of the aorta in the senium group, and thinner endometrium and regular and ordered arrangement of aortic vascular smooth muscles in both moxibustion and medication groups. Conclusion Mild moxibustion may improve vascular aging in senescence rats, which is possibly by suppressing vascular MCP-1/MMP-2/TGF-β1 pro-inflammatory signal loop.关键词：Mild moxibustion;Testosterone;Vascular aging;Aging18|574|2更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on learning-memory ability, ultrastructural changes of hippocampal CA1 neurons, reactive oxygen species(ROS) level, Nod-like receptor protein 3(NLRP3) and auto-phagy-related proteins expression in the hippocampus of vascular dementia(VD) rats, so as to reveal its partial mechanisms in treating VD. Methods Male SD rats were randomly divided into sham operation, model, and EA groups(n=10 rats in each group). The VD model was established by permanent ligation of bilateral common carotid arteries. Rats of the EA group were treated with EA at “Baihui”(GV20), “Dazhui”(GV14) and bilateral “Shenshu”(BL23) for 30 min, once a day for 4 weeks. Morris water maze was used to evaluate the learning and memory ability of rats before modeling, 4 weeks after modeling and after intervention. Transmission electron microscopy(TEM) was used to observe the ultrastructural changes of hippocampal CA1 neurons. The level of ROS in hippocampus was detected by DCFH-DA fluorescence probe. The expressions of NLRP3, autophagy-related protein Beclin1 and microtubule-associated protein 1 light chain 3(LC3) were measured by Western blot. Results In comparison with the sham operation group, the average escape latency of rats in the model group was prolonged(P<0.01), and the times of crossing the original platform were reduced(P<0.05), the level of ROS, the expression levels of LC3-Ⅱ/LC3-Ⅰ ratio, Beclin1 and NLRP3 proteins in hippocampus were increased(P<0.01, P<0.05) in the model group. After EA intervention, the average escape latency of rats was significantly shortened(P<0.01), and the times of crossing the original platform were increased(P<0.05), the level of ROS, the expression levels of LC3-Ⅱ/LC3-Ⅰ ratio, Beclin1 and NLRP3 proteins in hippocampus were decreased(P<0.01, P<0.05) in the EA group compared with those of the model group. Outcomes of TEM showed that CA1 neurons in the hippocampus were damaged, chromatin aggregation, mitochondria pyknosis, cristae structure disorder, rough endoplasmic reticulum expanded and degranulated, the number of free ribosomes decreased, and autophagy could be seen in the model group, which were milder in the EA group. Conclusion EA at GV20, GV14 and BL23 can improve the learning and memory abilities of VD rats, alleviate the ultrastructural damage of neurons in hippocampal CA1 area, and repair the damaged neurons. The mechanism may be related to the reduction of ROS level, LC3-Ⅱ/LC3-Ⅰ ratio, NLRP3 and Beclin1 protein expression, the decrease of neuronal autophagy, inhibition of activation of NLRP3 inflammasome and alleviation of central inflammatory response.关键词：Electroacupuncture;Vascular dementia;Autophagy;Nod-like receptor protein 3;Reactive oxygen species38|899|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) at “Zusanli”(ST36) and “Feishu”(BL13) on the activation and secretion of calcitonin gene-related peptide(CGRP) and 5-hydroxytryptamine(5-HT) of pulmonary neuroendocrine cells(PNECs) and inflammatory response in rats with chronic obstructive pulmonary disease(COPD), so as to explore its underlying mechanisms in treating COPD. Methods Male SD rats were randomly divided into normal control, COPD model and EA groups, with 7 rats in each group. The COPD model was established by forced inhale of cigarette smoke for 1 h in a self-made box(1 m×1 m×1 m in volume), twice daily for 12 weeks. EA(4 Hz/20 Hz, 1―3 mA) was applied at bilateral ST36 and BL13 acupoints for 30 min, once a day for 14 consecutive days. The pulmonary function including the forced vital capacity(FVC), forced expiratory volume at 0.1 second(FEV0.1), FEV0.3, FEV0.1/FVC and FEV0.3/FVC was detected using a lung function analyzer for small animals. The lung tissue was sampled for observing histopathological changes by using H.E. staining, for observing expression and distribution of PNECs by Grimelius silver staining, and for detecting the immunoactivity(integrated optical density) of CGRP and 5-HT by using immunohistochemistry. The contents of CGRP, 5-HT, tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and transforming growth factor-β1(TGF-β1) in the bronchoalveolar lavage fluid(BALF) and lung tissue were detected by ELISA, and the correlations between TNF-α and CGRP, IL-1β and CGRP, TNF-α and 5-HT, and IL-1β and 5-HT levels were analyzed. The mRNA and protein expression levels of nerve fiber markers of CGRP and purinergic receptor P2 X ligand gated ion channel 3(P2 X3) which dominate PNECs in the lung tissue were detected by real-time fluorescence quantitative PCR and Western blot, respectively. Results Compared with the normal control group, the levels of FVC, FEV0.1, FEV0.3, and the ratios of FEV0.1/FVC and FEV0.3/FVC were significantly decreased(P<0.05, P<0.01), while the immunoactivity of PNECs, CGRP and 5-HT, the contents of CGRP, 5-HT, TNF-α, IL-1β and TGF-β1 in the BALF and lung tissue, and the expression levels of CGRP and P2 X3 mRNAs and proteins in the lung tissue significantly increased in the COPD model group(P<0.01, P<0.05). Following EA intervention, both the increased and decreased levels of all the indexes mentioned above were reversed(P<0.05, P<0.01) except FEV0.3. H.E. staining showed severe deformed bronchial lumen with thickened wall and alveolar septum, and obvious inflammatory cell infiltration and reduced number of alveolar lumen fusion in the COPD model group, which was mild in the EA group. A positive correlation was found between TNF-α and CGRP, IL-1β and CGRP, TNF-α and 5-HT,IL-1β and 5-HT levels in both BALF and lung tissues(P<0.01). Conclusion EA at ST36 and BL13 can improve lung function and reduce inflammatory response in COPD rats, which may be related to its function in inhibiting the activation of PNECs and release of neuroactive substances.关键词：Electroacupuncture;Chronic obstructive pulmonary disease;Pulmonary neuroendocrine cells;Calcitonin gene related peptide;5-hydroxytryptamine;P2X3 receptor36|477|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) of “Shangjuxu”(ST37) and “Tianshu”(ST25) on colonic mucosal injury and activities of nuclear factor-κB(NF-κB)/Nod-like receptor family, pyrin domain-containing 3(NLRP3) inflammasome signaling in the colonic tissue in rats with ulcerative colitis(UC), so as to explore its mechanisms underlying improvement of UC. Methods Male SD rats were randomly divided into blank, model, medication and EA groups, with 12 rats in each group. The UC model was established by enema of 2-4-6 trinitrobenzene sulfonic acid +50% ethanol(2.5 mL). EA(10 Hz/50 Hz) was applied to bilateral ST37 and ST25 for 20 min, once a day, for a total of 10 days. Rats of the medication group received gavage of mesalazine suspension(2 mL:0.2 g/kg+0.9% saline) once a day, for 10 days. The rats' general conditions were recorded for calculating the disease activity index(DAI) score(0—4 points). The colonic tissue was sampled for giving colonic mucosa damage index(CMDI, 0—5 points) score and for observing histopathological changes after hematoxylin-eosin(HE) staining, and for detecting expression levels of NF-κB and NLRP3 by using immunohistochemistry and Western blot, separately. The contents of serum interleukin-1β(IL-1β), NLRP3 and tumor necrosis factor-α(TNF-α) were detected by enzyme-linked immunosorbent assay. Results Compared with the blank group, the DAI and CMDI scores, contents of serum IL-1β, NLRP3, and TNF-α, as well as the immunoactivity and expression of NF-κB and NLRP3 proteins were significantly increased in the model group(P<0.05). Relevant to the model group, modeling-induced increases of DAI and CMDI scores, serum IL-1β, NLRP3 and TNF-α contents, and NF-κB and NLRP3 expression were reversed in both medication and EA groups(P<0.05), the effect of EA was apparently superior to that of mesalazine in down-regulating CMDI score and serum IL-1β level(P<0.05). No significant diffe-rences were found between the medication and EA groups in down-regulating DAI score, serum TNF-α and NLRP3 contents, and expression of NF-κB and NLRP3 proteins(P>0.05). The rats' general conditions including arch back sloth, anorexia, loss of fur gloss, weight loss, lethargy and loose of stool, and histopathological changes such as necrosis of intestinal mucosa, formation of obvious ulcerative surface, with many neutrophils and pus cells and inflammatory cell infiltration were obvious in the model group, which were relative milder in both medication and EA groups. Conclusion EA can relieve colonic injury in UC rats, which may be related to its functions in down-regulating serum IL-1β, TNF-α and NLRP3 levels by suppressing colonic NF-κB/NLRP3 inflammasome signaling.关键词：Ulcerative colitis;Electroacupuncture;Nuclear transcription factor;NLRP3;Colonic tissue34|1190|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of warm needling on the expression of oxidative stress related factors and pro-inflammatory factors in cartilage of mono sodium iodoacetate(MIA) induced knee osteoarthritis(KOA) model rats, so as to explore its mechanisms underlying improvement of KOA. Methods Sixty male Sprague Dawley rats were randomly divided into 5 groups: control, model, acupuncture, moxibustion, warm needling, with 12 rats in each group. Rats of the acupuncture, moxibustion, warm needling groups received manual acupuncture or moxibustion or both stimulation of “Zusanli”(ST36) for 15 minutes, once a day for 21 days beginning from the third day after modeling. The foot volume was measured by drainage method, and the plantar mechanical contraction reflex threshold(mechanical pain threshold, MPT) measured by using an electronic pain meter. After 21 days of treatment, the histopathological changes of knee joint were observed by HE staining, and Mankin score was calculated to evaluate the degree of cartilage destruction. The malondialdehyde(MDA) level was measured by colorimetry, and immunofluorescence staining was used to observe the expression of NOX2, SOD2 or IL-1β. Results Compared with the control group, the knee joint swelling volume from the 3(rd) day after modeling, Mankin score, MDA level, and the number of NOX2 and IL-1β positive cells were significantly increased(P<0.01, P<0.05), while the MPT from the 3(rd) day after modeling, Mankin score, MDA level, and the number of NOX2 and IL-1β positive cells were significantly increased(P<0.01, P<0.05), while the MPT from the 3(rd) day after modeling, and the number of SOD2 positive cells were considerably decreased(P<0.01) in the model group. After the interventions, the increased levels of the knee joint swelling volume from the 12(rd) day after modeling, and the number of SOD2 positive cells were considerably decreased(P<0.01) in the model group. After the interventions, the increased levels of the knee joint swelling volume from the 12(th) day after modeling, and the Mankin score, MDA level, NOX2 and IL-1β positive cells, and the levels of decreased MPT from the 9(th) day after modeling, and the Mankin score, MDA level, NOX2 and IL-1β positive cells, and the levels of decreased MPT from the 9(th) day after modeling and SOD2 positive cell number were reversed in the acupuncture, moxibustion, warm needling groups(P<0.05, P<0.01), and the effects of warm needling were significantly superior to those of simple manual acupuncture and simple moxibustion in down-regulating knee joint volume, Mainkin score, MDA le-vel, and NOX2 and IL-1β positive cells, and in up-regulating MPT from the 12(th) day after modeling and SOD2 positive cell number were reversed in the acupuncture, moxibustion, warm needling groups(P<0.05, P<0.01), and the effects of warm needling were significantly superior to those of simple manual acupuncture and simple moxibustion in down-regulating knee joint volume, Mainkin score, MDA le-vel, and NOX2 and IL-1β positive cells, and in up-regulating MPT from the 12(th) day after modeling, and the number of SOD2 positive cells(P<0.05). No significant differences were found between the acupuncture and moxibustion groups in the levels of all the indexes mentioned above(P>0.05). HE staining showed rough and damaged articular surface, with subchondral neovascularization and moderate connective tissue hyperplasia, and abundant lymphocyte and monocyte infiltration in the model group, which was milder in the acupuncture, moxibustion groups particularly in the warm needling group after 21 days' interventions. Conclusion Warm needling can relieve knee joint pain, swelling and inflammatory damage in KOA rats, which may be associated with its function in inhibiting oxidative stress and inflammation in the cartilage of KOA. The therapeutic effect of warm needling is better than that of manual acupuncture and moxibustion alone.关键词：Warm acupuncture;Knee osteoarthritis;Pain;Oxidative stress;Inflammation28|1252|6更新时间：2023-08-11
- 摘要：Objective To observe the effects of eye acupuncture on motor evoked potential(MEP) and somatosensory evoked potential(SEP) in the patients with incomplete spinal cord injury so as to evaluate its clinical efficacy. Methods According to the random number table, 90 patients were divided into exercise therapy group, eye acupuncture group and eye acupuncture combined exercise therapy group(combined treatment group), 30 cases in each. In the exercise therapy group, patients were treated with the routine exercise and occupational therapy. Patients of the eye acupuncture group were treated with eye acupuncture at upper jiao region, lower jiao region, liver region and kidney region bilaterally. Patients of the combined treatment group were given the routine exercise and occupational therapy combined with eye acupuncture. All the treatments were conducted once daily, 7 days as one treatment course for 4 treatment courses. Before treatment and 4 weeks after treatment, the motor function, light touch sensation and pinprick sensation, injury grade and clinical efficacy were assessed separately, using the criteria developed by the American Spinal Injury Association. The modified Barthel index(MBI) was adopted to evaluate the activities of daily livings. By monitoring SEP and MEP, the neurophysiological conditions were assessed for spinal cord injury. Results The total effective rate was 56.7%(17/30), 66.7%(20/30) and 90.0%(27/30) in the exercise therapy group, the eye acupuncture group and the combined treatment group, respectively. The total effective rate in the combined treatment group was higher than those in the other two groups(P<0.05). Compared with those before treatment, the scores of motor function, light tough sensation and pinprick sensation were all increased after treatment in three groups(P<0.05), MBI score was increased in both the exercise therapy group and the combined treatment group(P<0.05), and the latency of SEP(N11, N20, N23, P38) and the Cortical(hand region), Csp, Cortical(leg region) and Lsp of MEP were all shortened in the three groups separately(P<0.05). After treatment, compared with the exercise therapy group, the score of motor function was increased(P<0.05), MBI score decreased(P<0.05) and MEP latency shortened(P<0.05) in the eye acupuncture group. After treatment, compared with the exercise therapy group and the eye acupuncture group, the scores of motor function, light touch sensation and pinprick sensation, as well as MBI score were all increased(P<0.05), and the latency of SEP(N11,N20,N23,P38) and MEP shortened(P<0.05) in the combined treatment group. Conclusion In treatment of incomplete spinal cord injury, eye acupuncture combined with exercise therapy can significantly increase the excitability of sensory and motor nerve conduction in the spinal cord and cerebral cortex of patients, effectively promote the recovery of patients' motor and sensory function and improve the activities of daily living.关键词：Eye acupuncture;Neuroelectrophysiology;Spinal cord injury;Motor evoked potential;Somatosensory evoked potential22|384|3更新时间：2023-08-11
- 摘要：Objective To evaluate the clinical efficacy and safety of acupuncture combined with moxibustion on allergic rhinitis. Methods Using the random number table, 80 patients with allergic rhinitis were divided into a medication group and an acupuncture combined with moxibustion(acu-mox) group, 40 cases in each one. In the medication group, ioratadine tables were prescribed for oral administration, one tablet daily for 10 days as 1 session, 3 sessions of treatment were required. In the acupuncture combined with moxibustion group, bilateral Yingxiang(LI20), Yintang(EX-HN3), bilateral Hegu(LI4) and bilateral Shenshu(BL23) were selected as the main points and stimulated with acupuncture and moxibustion; and the acupoint prescription was modified according to symptoms. This combined treatment was given once every day, stimulating for 30 min each time, and 10 treatments made 1 course, for 3 courses of treatment totally. Before and after treatment, the scores for symptoms and physical signs, as well as the score of rhino-conjunctivitis related quality of life scale(R-QOL) were evaluated separately. The sample of the inferior turbinate mucosa tissue was collected and the distribution of eosinophil(EOS) was scored using HE staining and Sheldeny evaluation. Using enzyme-linked immunosorbent assay(ELISA), the contents of serum immunoglobulin E(IgE), retinoic-acid-receptor-related orphan nuclear receptor γt(RORγt), forkhead box protein P3(Foxp3), interleukin-17(IL-17), IL-27 and IL-33 were determined. The clinical efficacy was evaluated in the patients with allergic rhinitis of two groups and all the adverse reactions were recorded during treatment. Results The scores of symptoms and physical signs as well as the score of R-QOL, and EOS distribution score and the contents of serum IgE, RORγt, IL-17 and IL-33 were all reduced as compared with those before treatment in each group(P<0.05), and the contents of serum Foxp3 and IL-27 were increased as compared with those before treatment in each group(P<0.05). After treatment, the scores of symptoms and physical signs as well as the score of R-QOL, and the contents of serum IgE, RORγt and IL-33 in the acu-mox group were lower than those in the medication group(P<0.05), and the contents of serum Foxp3 and IL-27 were higher than those of the medication group(P<0.05). The total effective rate of the acu-mox group was 100.0%(40/40), significantly higher than 82.5%(33/40) in the medication group(P<0.05). No ob-vious adverse reaction was found in either group during and after treatment. Conclusion Acupuncture combined with moxibustion is significantly effective and safe in treatment of allergic rhinitis. Its effect mechanism may be related to the balance modulation of Th1/Th2 and Th17/Treg cells mediated by naive CD4+T cells.关键词：Acupuncture combined with moxibustion;Allergic rhinitis;Clinical efficacy;Safety evaluation21|1070|7更新时间：2023-08-11
- 摘要：Objective To observe the clinical curative effect of wentongzhenfa(warming and promoting technique of acupuncture) combined with extracorporeal shock wave in treatment of type Ⅲ prostatitis. Methods A total of 96 patients with type Ⅲ prostatitis were randomly divided into an observation group(48 cases) and a control group(48 cases). In the control group, the extracorporeal shock wave was combined with even-needling technique of acupuncture at Guanyuan(CV4), Mingmen(GV4), Zhongji(CV3), Zusanli(ST36), etc. In the observation group, the extracorporeal shock wave was combined with “warming and promoting technique” of acupuncture at the same acupoints as the control group. The treatment lasted 30 min each time, once daily in either group. There were 2 days of interval after consecutive treatment for 5 days. Totally, the duration of treatment was 1 month in two groups. The clinical curative effect was assessed after treatment. Before and after treatment, the changes in the concentrations of tumor nerosis factor-α(TNF-α), interleukin-1β(IL-1β) and IL-6 in prostatic fluid were determined; and the symptoms were scored, i.e. frequent, urgent and burning painful urine, difficulty in urination, dribbling urine, distending pain in perineum, bitter taste and dry mouth, and scrotal dampness. The changes in the scores of National Institute of Health chronic prostatitis symptom index(NIH-CPSI), international index of erectile function(IIEF), visual analogue scale(VAS) were evaluated befroe and after treatment. Successively, before treatment, after treatment, as well as 1 and 3 months after treatment, the quality of life was evaluated by Karnofsky in the patients of two groups. Results After treatment, the total effective rate was 89.6%(43/48) in the observation group, higher than 70.8%(34/48) in the control group(P<0.05). Compared with those before treatment, the concentrations of TNF-α and IL-1β in the prostatic fluid were all decreased(P<0.05) and the concentration of IL-6 was increased(P<0.05), the scores of symptoms, NIH-CPSI, IIEF and VAS were all reduced(P<0.05) in two groups. The changes of the above indexes were more obvious in the observation group than those in the control group(P<0.05). After treatment and 1 and 3 months after treatment, Karnofsky scores all increased(P<0.05) in two groups, and the increases were more significant in the observation group(P<0.05). Conclusion “Warming and promoting technique” of acupuncture combined with extracorporeal shock wave promotes the elimination of local inflammatory factors, relieves clinical symptoms, improves the quality of life, as well as has a satisfactory short-term and medium-term curative effect on type Ⅲ prostatitis.关键词：“Warming and promoting technique” of acupuncture;Extracorporeal shock wave;Type Ⅲ prostatitis25|301|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of thunder-fire moxibustion combined with meibomian gland massage in improving meibomian gland dysfunction(MGD) and explore its mechanism. Methods Seventy-two MGD patients with 144 eyes in the Jinhua Hospital of Traditional Chinese Medicine from February 2019 to January 2021 were selected and randomly divided into an experimental group(n=36,72 eyes) and a control group(n=36, 72 eyes). Patients in the control group received 0.1% fluo-rometholone eye drops and 0.1% sodium hyaluronate eye drops, 1-2 drops per time, four times per day, and the meibomian glands were massaged once per day. Patients in the experimental group received additional thunder-fire moxibustion on the basis of the treatment of the control group, 10 cones per time, once per day. One month after treatment, meibomian gland function was assessed, and the levels of interleukin-6(IL-6) and prostaglandin E_2(PGE_2) in tears were detected. Results After treatment, the scores of Ocular Surface Disease Index, meibomian hyperemia, meibomian gland opening, meibomian gland loss, and meibomian gland secretion function were lower than those before treatment in the two groups, and the scores of the experimental group were lower than those of the control group(P<0.05). After treatment, the tear break-up time and tear meniscus height were higher than those before treatment in the two groups, which were higher in the experimental group than those in the control group(P<0.05). The post-treatment levels of IL-6 and PGE_2 were lower than those before treatment in the two groups, and the levels in the experimental group were lower than those in the control group(P<0.05). Conclusion Thunder-fire moxibustion combined with meibomian gland massage can significantly improve the function of the meibomian glands and lower the levels of IL-6 and PGE_2 in tears.关键词：Thunder-fire moxibustion;Meibomian gland massage;Meibomian gland dysfunction(MGD);Interleukin-6(IL-6);Prostaglandin E_2(PGE_2)26|414|0更新时间：2023-08-11
- 摘要：Acupuncture can intervene in and treat ischemic stroke(IS) through multiple targets and pathways. In recent years, experimental studies have shown that the mechanism of acupuncture in the treatment of IS mainly involves the promotion of nerve repair and regeneration, the regulation of the activity of neurotransmitters, the improvement of cerebral blood flow, the alleviation of brain edema, the reduction of oxidative stress, the inhibition of apoptosis and autophagy, and the suppression of inflammatory responses. This paper reviewed the available mechanisms of acupuncture in the treatment of IS to provide theoretical support for the clinical application of acupuncture against IS and references for further research.关键词：Acupuncture;Ischemic stroke;Mechanism;Review51|2151|10更新时间：2023-08-11
- 摘要：Alzheimer's disease(AD) is a common neurodegenerative disease in the elderly. Studies have shown that acupuncture can effectively alleviate the symptoms of AD and slow down the disease progression, and its mechanism has been intensively explored. This paper summarized the researches on the mechanism of acupuncture intervention on AD in the past 5 years. It has been found that acupuncture intervention on AD is correlated with the regulation of the expression levels of relevant proteins, the inhibition of central inflammatory response, the resistance to oxidative stress injury, the modulation of brain energy metabolism, the improvement of neuronal synaptic plasticity, the adjustment of autophagy activity and the suppression of neuronal apoptosis.关键词：Alzheimer Disease;Acupuncture;Mechanism research;Review39|2087|6更新时间：2023-08-11
- 摘要：Objective To systematically review the occurrence of adverse events/adverse reactions(AEs/ARs) induced by acupoint catgut embedding therapy for psoriasis vulgaris(PV) and its safety. Methods Randomized controlled trials, controlled clinical trials, cohort studies, case-control studies, case-series, and case reports concerning the treatment of PV with acupoint catgut embedding therapy were searched from Chinese and English databases from their inception to January 7(th), 2021. The AEs/ARs related to acupoint catgut embedding therapy for PV were subjected to descriptive statistics, followed by the analysis of possible reasons. Results Finally, 16 studies were included, involving 1 158 patients. A total of 79 cases were reported to present with mild to moderate AEs/ARs related to acupoint catgut embedding therapy for PV, and there were no serious AEs/ARs or death cases. The most common AEs/ARs were local redness, swelling, heat, and pain(31.65%,25/79), followed by low-grade fever and fatigue(29.11%,23/79), isomorphic reaction(16.46%,13/79), local induration(13.92%,11/79), and fainting(8.86%,7/79). In terms of embedding materials, catgut(93.67%,74/79) and lumbar puncture needles or other puncture needles(49.37%,39/79) were proved the most common AEs/ARs-inducing factors. The proportion of AEs/ARs resulting from treatment interval≤two weeks(67.09%,53/79) and treatment course≤eight weeks(55.70%,44/79) was relatively high. Because the incidence of AEs/ARs fails to be calcula-ted, it is not yet possible to accurately assess the risk and safety of acupoint catgut embedding therapy for PV. Conclusion Available evidence suggests that in the treatment of PV, acupoint catgut embedding therapy may induce a series of mild to moderate AEs/ARs, so its clinical practice deserves attention. We should strictly grasp its indications and contraindications, and prevent the occurrence of related AEs/ARs by standardizing the operation and improving the embedding materials.关键词：Acupoint catgut embedding;Psoriasis vulgaris(PV);Adverse events/adverse reactions;Systematic review22|618|0更新时间：2023-08-11
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