最新刊期
卷 47 ， 期 5 ， 2022
- 摘要：Objective To compare the effect of electroacupuncture(EA) on “Guanyuan”(CV4) or sensitization points in mice with polycystic ovary syndrome(PCOS), so as to explore its mechanisms underlying improvement of PCOS. Methods In the first part of this study, 26 female ICR mice were randomized into control group(8 mice) and model group(18 mice). The PCOS model was established by gavage of bisphenol A(BPA) at a dose of 100 mg/kg, and the control group were gavage of equal volume of corn oil, once daily, 5 days a week for 4 conse-cutive weeks. Evans blue(EB) dye(0.1 mL/10 g) was injected into the caudal vein after modeling. The size, number and distribution of EB exudation points at the skin were observed. In the second part of this study, 32 mice were randomized into control, model, EA-CV4 and EA-sensitization points groups(8 mice in each group). EA(2 Hz, 2 mA) was applied to the sensitization points or CV4 for 20 min, once daily, 5 days a week for 4 conse-cutive weeks. The body weight was measured once a week for 8 consecutive weeks. The behavior changes were evaluated by open field test and elevated plus maze test. H.E. staining was used to observe the histopathologic changes of the ovary tissue. Serum level of estradiol(E_2) was measured by ELISA. The expressions of estrogen receptor α(ER-α) in ovarian and uterine tissues were detected by Western blot and immunofluorescence. Results(1) In PCOS mice, the EB exudation points were found to overlap the lower abdomen, lumbosacral, chest, back and lower limbs regions, and the number of EB points was significantly more than that of the control group(P<0.01).(2) After the intervention and compared with the control group, the ovaries showed polycystic changes and an increase of atresia follicles with a larger diameter, the activity time in the central area, the total distance of movement, the times of open-arm entries, the duration in open-arm, the serum E_2 content and the expression of ER-α in ovarian tissue were significantly decreased(P<0.01, P<0.05), while the mice's body weight and the expression of ER-α in uterine tissue were increased(P<0.05) in the model group. After the intervention and compared with the model group, a small number of normal follicles and corpus luteum were observed under microscope, the activity time in the central area, the total distance of movement, the times of open-arm entries, the duration in open-arm, the serum E_2 content and the expression of ER-α in ovarian tissue were significantly increased(P<0.05, P<0.01), while the mice's body weight and the expression of ER-α in uterine tissue was decreased(P<0.05) in both EA-CV4 and EA-sensitization points groups. Conclusion EA at sensitization points and CV4 can regulate the expression of estrogen and ER-α in PCOS mice, and improve the anxiety like behavior. EB exudation points on the body surface can not only reflect the functional changes of organs, but also treat diseases through body surface stimulation, suggesting a dual role in diagnosis and treatment.关键词：Polycystic ovary syndrome;Acupoint sensitization;Conception Vessel;Anxiety-like behavior;Estrogen receptor α52|759|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) at “Zusanli”(ST36) on apoptosis of intestinal T lymphocytes, translocation of intestinal bacteria and expression of intestinal Bcl-2 and Bax proteins and intestinal mucosal immune barrier in sepsis rats, so as to explore its underlying mechanism in relieving sepsis. Methods SD rats were randomly divided into sham operation(n=6), model(n=15), non-meridian and non-acupoint(non-acupoint, n=15) and acupoint EA(n=15) groups by using random number table method. The sepsis model was established by using cecal ligation and perforation(CLP) method. EA(2 Hz, 2 mA) was applied to bilateral ST36 or non-acupoint for 30 min one hour after modeling, once every day for 3 days. The rats' general conditions and fatality rate in 3 days after modeling were recorded. The liver, spleen and mesenteric lymph nodes were taken for bacterial culture to detect the translocation rate of intestinal bacteria. The small intestinal tissue was taken for observing histopathological changes(Chiu's score: 0-5 points) after HE staining, and for determining the expression levels of Bcl-2 and Bax proteins using Western blot. The intestinal mucosa was sampled for detecting the apop-tosis(apoptotic index) of lymphocytes by using terminal deoxynucleoitidyl transferase-mediated deoxyuridine triphosphate biotin nick end labeling(TUNEL) assay, and the counts of CD4+ and CD8+ and CD8+T cells using flow cytometry. The contents of IL-4 in the small intestine and that of secretory IgA(sIgA) in the small intestinal mucus were determined by using ELISA. Results After modeling, of the 15 rats in each of the 3 groups, 7, 7 and 2 in the model, non-acupoint and EA groups were dead in the first 3 days, with the fatality rate being 46.67%(7/15), 46.67%(7/15) and 13.33%(2/15), respectively(being obviously lower in the EA group than in the former two groups, P<0.05). Compared with the sham operation group, the incidence of intestinal bacterial translocation, apoptotic index, Chiu's score, and Bax expression were significantly increased(P<0.05), and the percentages of CD4+T cells using flow cytometry. The contents of IL-4 in the small intestine and that of secretory IgA(sIgA) in the small intestinal mucus were determined by using ELISA. Results After modeling, of the 15 rats in each of the 3 groups, 7, 7 and 2 in the model, non-acupoint and EA groups were dead in the first 3 days, with the fatality rate being 46.67%(7/15), 46.67%(7/15) and 13.33%(2/15), respectively(being obviously lower in the EA group than in the former two groups, P<0.05). Compared with the sham operation group, the incidence of intestinal bacterial translocation, apoptotic index, Chiu's score, and Bax expression were significantly increased(P<0.05), and the percentages of CD4+ and CD8+ and CD8+T cells, IL-4 and sIgA contents and Bcl-2 expression considerably decreased(P<0.05) in the model group. In comparison with the model group, modeling-induced increase of incidence of bacterial translocation, apoptotic index and Bax expression, and decrease of percentages of CD4+T cells, IL-4 and sIgA contents and Bcl-2 expression considerably decreased(P<0.05) in the model group. In comparison with the model group, modeling-induced increase of incidence of bacterial translocation, apoptotic index and Bax expression, and decrease of percentages of CD4+ and CD8+ and CD8+T cells, IL-4 and sIgA contents and Bcl-2 expression were reversed(P<0.05) in the EA group. Conclusion EA at ST36 can reduce death rate and intestinal bacteria translocation incidence in sepsis rats, which may be related to its functions in regulating the expression of intestinal Bcl-2 and Bax proteins and inhibiting the apoptosis of intestinal mucosal T lymphocytes, thereby protecting the immune barrier function of intestinal mucosa to reduce the intestinal permeability.关键词：Electroacupuncture;Sepsis;Intestinal immune barrier;lymphocyte;Apoptosis;Bacteria translocation48|623|7更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on ocular surface sensory neuralgia and the expression of P2 X_3 receptor(P2 X_3R) and protein kinase C(PKC)in cornea and trigeminal ganglion(TG) in dry eye disease(DED) guinea pigs, so as to explore its mechanism underlying improvement of ocular surface sensory neuralgia in DED. Methods Male British tricolor short haired guinea pigs were randomly divided into control, model, medication(pranoprofen), EA and sham acupuncture groups, with 8 guinea pigs in each group. The dry eye model was induced by subcutaneous injection of scopolamine hydrobromide solution(0.6 mg/0.2 mL,once daily) for 10 d. Guinea pigs in the medication group were treated by applying pranoprofen eye drops to eyes, 1 drop for one eye each time, three times a day. Guinea pigs of the EA group received EA stimulation(4 Hz/20 Hz, 1 mA) of bilateral “Cuanzhu”(BL2) and “Taiyang”(HN5) and acupuncture at “Jingming”(BL1) “Sizhukong”(TE23), “Tongziliao”(GB1) for 15 min, once a day. Guinea pigs in the sham acupuncture group received blunt stimu-lation at the surface of the same acupoint with the tip of the acupuncture needle, once a day. All the treatments were conducted for 14 d. The corneal epithelium fluorescein staining score(0-3 points) was given according to the number of fluorescence-positive dots and flake-like coloration, the corneal mechanical perception thread(CMPT) detected using a corneal perception meter, and the palpebral fissure height measured. The number of sensory neurons in the cornea and TG was determined by using cholera toxin subunit B conjugated with Alexa Fluor 488 fluorescence labelling, and the expression levels of P2 X_3R and PKC in the cornea and TG detected by using immunohistochemistry and Western blot, separately. Results Compared with the control group, the corneal fluorescein staining score, immunoactivity and expression of P2 X_3R proteins in both cornea and TG, PKC proteins in TG were significantly increased(P<0.01), whereas the CMPT and the height of palpebral fissure and the number of TG neurons significantly decreased in the model group(P<0.05,P<0.01). In comparison with the model group, the fluorescein staining score in the medication and EA groups, the immunoactivity and expression of P2 X_3R in cornea and TG in the EA group, and that of TG PKC in the EA group and the sham acupuncture groups were significantly decreased(P<0.05, P<0.01), while the height of palpebral fissure and CMPT after EA and the number of labelling TG sensory neurons were remarkably increased in the EA group(P<0.01) rather than in the medication and sham acupuncture groups(P>0.05). Conclusion EA can alleviate the damage of corneal epithelium and sensory neurons in dry eye model guinea pigs, which may be related to its functions in down-regu-lating the expression of P2 X_3R and PKC in the cornea and TG.关键词：Electroacupuncture;Dry eye;Sensory neuralgia;P2X_3 receptor;Protein kinase C43|300|2更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion treatment on the expression of Nogo-A, Nogo receptor(NgR), neurotrophin receptor p75(p75 NTR) and leucine rich repeat and Ig domain containing 1(Lingo-1) in brain tissue of rats with cerebral ischemia/reperfusion injury(CI/RI), so as to analyze its mechanism underlying improvement of CI/RI. Methods Male SD rats were randomly divided into sham operation group(16 rats), model group(17 rats), NEP1-40(extracellular peptide residues 1-40, a blocker targeting NgR) group(model+blocker, 17 rats) and moxibustion group(model+moxibustion, 17 rats). The CI/RI model was established by occlusion of the left middle cerebral artery(MCAO). Moxibustion was applied to “Baihui”(GV20), right “Quchi”(LI11) and “Zusanli”(ST36) for 20 min, once a day for 14 days, with 2 days' rest after the top 7 days' intervention. For rats of the NEP1-40 group, 30 μL PBS containing 18 μg NEP 1-40 was injected into the epidural inferior vena(L5-S1) via a polyvinyl chloride conduit. The neurological deficit state in each group was evaluated by Longa's 5-point scale and Feeney's 7-point scale of beam walking test(BWT). The cerebral infarct volume was assessed by 2,3,5-triphenyltetrazole chloride staining. The brain tissue between the central anterior and posterior sulcus was taken for observing the expression of NgR and Lingo-1 by fluorescence double-label method, and for determining the expression levels of Nogo-A, NgR, p75 NTR and Lingo-1 mRNAs and proteins by real-time quantitative PCR and Western blot, respectively. Results After modeling, the Longa's score, infarct volu-me percent, expression levels of Nogo-A, NgR, Lingo-1 and p75 NTR mRNAs and proteins were significantly increased(P<0.01) and BWT score was obviously decreased(P<0.01) in the model group relevant to the sham operation group. In comparison with the model group, the increase of Longa's score, infarct volume percentage, expression levels of Nogo-A, NgR, Lingo-1 and p75 NTR mRNAs and proteins and decrease of BWT score in NEP1-40 and moxibustion groups were reversed(P<0.01) except Nogo-A protein in the NEP1-40 group. The effect of moxibustion was significantly superior to that of blocker NEP1-40 in redu-cing the infarct volume percentage, and down-regulating the expression of Nogo-A mRNA and protein, p75 NTR mRNA and protein, NgR and Lingo-1 proteins(P<0.01, P<0.05). Conclusion Moxibustion, similar to blocker NEP1-40 of NgR, can improve neurological dysfunction in CI/RI rats, which may be related to its functions in reducing cerebral infarction and down-regulating the activity of Nogo/neurotrophin receptor signaling pathway.关键词：Cerebral ischemia/reperfusion injury;Moxibustion;Neurological deficit;Cerebral infarct volume;Nogo/neurotrophin receptor signaling pathway35|593|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion, catgut embedding and acupuncture on allergic symptoms and expression of interferon-γ(IFN-γ) and interleukin-4(IL-4) of nasal mucosa in rats with allergic rhinitis(AR) based on acupoint injection, so as to explore their synergistic effect and related mechanism in relieving AR. Methods SD rats(half male half female) were randomly divided into normal control, model, acupoint injection(AI), AI+moxibustion, AI+catgut embedding and AI+acupuncture groups, with 8 rats in each group. The AR model was established by intraperitoneal injection of ovalbumin suspension(once every other day for 7 times), and intranasal drop of 0.5% ovalbumin solution(once daily for 7 days). After successful modeling, rats of the AI group received injection of a mixture solution of equal proportion of 1% lidocaine, dexamethasone and transfer factor into “Yingxiang”(LI20) and “Yintang”(EX-HN3) once every 4 days, 4 times altogether. Mild moxibustion or catgut embedment or manual acupuncture was applied to bilateral “Feishu”(BL13) and “Zusanli”(ST36). Both moxibustion(20 min every time) and acupuncture(with the needles retained for 30 min every time) were conducted once daily for 14 times, and catgut embedding was conducted once a week, twice altogether based on acupoint injection. The rats' nasal allergic reaction score(symptom score, 1—3 points) was given according to the times of nose scratching and sneezing, and the running nose state in 30 min, and histopathological changes of nasal mucosa were observed by H.E. staining. The expression levels of IFN-γ and IL-4 in the nasal mucosa were detected by immunohistochemistry and Western blot, separately. Results Compared with the normal group, the symptom score and the expression of IL-4 positive cells and protein in nasal mucosa were significantly increased(P<0.05), while the expression of IFN-γ positive cells and protein were considerably decreased in the model group(P<0.05). In comparison with the model group, the symptom score and the expression of IL-4 positive cells and protein were obviously decreased(P<0.05), while the expression of IFN-γ positive cells and protein were remarkably increased in the 4 treatment groups(P<0.05). The effects of AI+moxibustion, AI+catgut embedment, AI+acupuncture were signi-ficantly superior to those of simple AI in up-regulating the expression of IFN-γ positive cells and protein and in down-regulating the expression of IL-4 positive cells and protein(P<0.05). Both the symptom score and the expression of IL-4 were notably lower in the AI+moxibustion group than in the AI+catgut embedment and AI+acupuncture groups(P<0.05), whereas the expression of IFN-γ was apparently higher in the AI+moxibustion group than in the other 3 treatment groups(P<0.05). No significant differences were found between the AI+catgut embedment and AI+acupuncture groups in the levels of symptom score, IFN-γ and IL-4 expressions(P>0.05). Conclusion Moxibustion or catgut embedment or acupuncture and AI have a synergistic effect in relieving symptoms of AR rats, which may be related to their function in regulating the expression levels of nasal IFN-γ and IL-4 proteins. The therapeutic effect of moxibustion is obviously superior to those of both acupuncture and catgut embedment.关键词：Allergic rhinitis;Acupoint injection;Synergistic effect;Interleukin-4;Interferon-γ45|557|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of acupuncture on the expression of miR-34 c-5 p, autophagy-related proteins and apoptosis rate in hippocampus of rats with cerebral ischemia/reperfusion injury(CI/RI), so as to explore its mechanism in regulating autophagy in hippocampal neurons in CI/RI rats. Methods SD rats were randomly divided into sham operation, model, medication and acupuncture groups, with 20 rats in each group. The rat model of CI/RI was established by occlusion of the middle cerebral artery. In the acupuncture group, “Dazhui”(GV14), “Baihui”(GV20) and “Shuigou”(GV26) were punctured with filiform needles and stimulated manually once every 15 min, for 30 min. The rats of the medication group were intraperito-neally injected with edaravone(5 mg/kg). The treatment was conducted once every 12 h for a total of 7 times. The neurological de-ficit score of all the rats were evaluated according to Garcia's methods, and TTC staining was employed to assess the cerebral ischemic area(percentage of cerebral infarct area, CIA). Transmission electron microscopy(TEM) was used to observe the ultrastructural changes of hippocampal neurons. The expression of hippocampal miR-34 c-5 p was measured by real-time PCR, and the protein expressions of hippocampal LC3 B, Beclin1 and p62 were measured by Western blot. The apoptosis rate of ischemic brain tissue was observed by TUNEL staining. Results Compared with the sham operation group, the neurological deficit score, the expressions of miR-34 c-5 p, Beclin1, p62 and LC3 B-Ⅱ/LC3 B-Ⅰ were significantly decreased(P<0.01, P<0.05), and the CIA and the apoptosis rate significantly increased(P<0.01) in the model group. Compared with the model group, the neurological deficit scores, the expressions of miR-34 c-5 p, Beclin1, p62 and LC3 B-Ⅱ/LC3 B-Ⅰ were significantly increased(P<0.01, P<0.05), and the CIA and the apoptosis rate significantly decreased(P<0.01) in the medication and acupuncture groups. Compared with the medication group, the expression of miR-34 c-5 p was significantly increased(P<0.01). The results of electron microscope showed that the neurons in the acupuncture and medication groups were less damaged than those in the model group, the cells showed mild edema, and the structures were relatively complete. Some normal organelles could be seen, and autophagy bodies, autophagy lysosomes and their encapsulated organelles could still be observed. Conclusion Acupuncture can improve the neurological deficit and reduce the area of cerebral infarction in CI/RI rats, which is closely with its effect in promoting hippocampal neuronal autophagy and anti-apoptosis via up-regulating the expression of miR-34 c-5 p.关键词：Acupuncture;miR-34c-5p;Cerebral ischemia/reperfusion injury;Autophagy38|772|10更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the expression level of Caspase-3, so as to explore its mechanism in inhibiting apoptosis after cerebral ischemia reperfusion. Methods SD male rats were randomly divided into sham-operation, model, EA and Caspase-3 inhibitor groups(n=20 rats in each group). The focal cerebral ischemia/reperfusion injury rat model was established by occlusion of the middle cerebral artery. Rats of the EA group received EA at “Hegu”(LI4), “Chize”(LU5), “Zusanli”(ST36) and “Sanyinjiao”(SP6) on the affected side for 20 min. Rats of the inhibitor group were given intracerebroventricular injection of inhibitor Z-DEVD-FMK 5 μg before modeling. The neurological deficit scores(NDS) were assessed by using Longa's method, the infarct size of the brain assessed after staining with 2% triphenyltetrazolium chloride. The apoptosis index of nerve cells were observed by TUNEL staining, PCR and Western blot were used to detect the mRNA and protein expressions of Caspase-3 in the hippocampus, separately. Results After modeling, the NDS, infarct volume, the apoptosis index of hippocampus CA1 area, and Caspase-3 mRNA and protein expression levels were significantly increased in the model group compared with the sham-operation group(P<0.01). After intervention, the NDS, infarct volume, the apoptosis index, Caspase-3 mRNA and protein expression levels were all significantly decreased in the EA and Caspase-3 inhibitor groups re-levant to the model group(P<0.05). Conclusion EA can improve the neurological function in cerebral ischemia/reperfusion rats, which may be related to its effect in inhibiting of Caspase-3 expression.关键词：Electroacupuncture;Cerebral ischemia/reperfusion injury;Apoptosis;Caspase-332|1141|4更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the expression of peroxisome proliferator-activated receptor gamma coactivators-1-alpha(PGC-1α), Irisin and brain-derived neurotrophic factor(BDNF) in the ischemic peripheral cortex, hippocampus and local skeletal muscle in rats with focal cerebral ischemic/reperfusion injury(CI/RI), so as to explore its underlying mechanism of improving of CI/RI. Methods Male SD rats were randomly divided into 3 groups: sham-operation, model and EA(11 rats in each group). The focal CI/RI model was established by middle cerebral artery occlusion(MCAO). EA(2 Hz/15 Hz, 2 to 4 mA) was applied to “Quchi”(LI11) and “Zusanli”(ST36) of the affected side for 20 min, once a day for 7 days. Zea-Longa's score and Balance Beam score were used to evaluate the neurological and motor functions. The infarcted volume of the brain was detected by using 2,3,5-triphenyltetrazolium chloride staining. The expression levels of PGC-1α, fibronectin type III domain-containing protein 5(FNDC5) and BDNF proteins in the ischemic peripheral cortex, hippocampus and local skeletal muscle were detected by Western blot. Results Compared with the sham-operation group, the Zea-Longa's score, Balance Beam score, percentage of cerebral infarct volume were notably increased(P<0.01), while the expression levels of PGC-1α, FNDC5 and BDNF proteins in the cerebral cortex and hippocampus(not in the local muscle) were significantly down-regulated in the model group(P<0.01, P<0.05). In comparison with the model group, the increase of Zea-Longa's score, Balance Beam score, percentage of cerebral infarct volume, and the decrease of expression levels of PGC-1α, FNDC5 and BDNF proteins in the ischemic peripheral cortex and that of BDNF in the hippocampus were reversed in the EA group(P<0.01, P<0.05). No significant changes were found in the expression levels of hippocampal PGC-1α and FNDC5 proteins in the hippocampus and those of PGC-1α, FNDC5 and BDNF proteins in the local muscle after EA intervention(P>0.05). Conclusion EA can improve neurological and motor functions and reduce cerebral infarction volume in CI/RI rats, which may be related to its functions in activating PGC-1α/Irisin(FNDC5)/BDNF pathway in the cerebral cortex.关键词：Electroacupuncture;Cerebral ischemic/reperfusion injury;Peroxisome proliferator-activated receptor gamma coactivators-1-alpha;Irisin;Brain-derived neurotrophic factor55|580|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) of combined “Biao”-and “Ben”-acupoint(for treating symptoms and root causes of the disease, respectively) on the expression of kidney forkhead box O1(FoxO1) and peroxi-some proliferator-activated receptor-γ coactivator-1α(PGC-1α) in diabetic nephropathy(DN) rats, so as to explore its potential mechanisms underlying improvement of DN. Methods Wistar rats were randomly divided into normal control(n=10), DN model(n=12), EA(n=11), EA+inhibitor(AS1842856 targeting FoxO1, n=11) and inhibitor(n=11) groups. The DN model was established by high fat and high glucose diet for 6 weeks and intraperitoneal injection of streptozotocin(55 mg/kg). EA(2 Hz, 1 mA) was applied to bilateral “Zusanli”(ST36), “Guanyuan”(CV4), “Fenglong”(ST40) and “Zhongwan”(CV12) for 15 min, once every other day for 8 weeks. The body mass was recorded, and blood glucose detected. The serum was sampled for detecting creatinine(Scr) content with Jaffe's assay, urea nitrogen(BUN) content with urease method. Urine albumin(ALB) and renal reactive oxygen species(ROS) contents were detected with ELISA, renal superoxide dismutase(SOD) activity with xanthine oxidase method, and renal malondialdehyde(MDA) content with thiobarbituric acid method. The renal subcellular structure was observed under transmission electron microscopy, and the expression levels of PGC-1α and FoxO1 proteins in the kidney tissue were detected using Western blot. Results Compared with the normal control group, the levels of body mass, SOD activity, and FoxO1 and PGC-1α protein expression were significantly reduced(P<0.01), while the contents of blood glucose, and serum Scr and BUN, urine ALB, renal MDA and ROS levels significantly increased in the model group(P<0.01). In comparison with the model group, the levels of body mass, SOD activity, and FoxO1 and PGC-1α expression were significantly increased in the three treatment groups except SOD, expression of FoxO1 and PGC-1α in the inhibitor group(P<0.01, P<0.05), and the contents of blood glucose, Scr, BUN, ALB, MDA and ROS were obviously decreased in the three treatment groups except ALB and ROS in the inhibitor group(P<0.01, P<0.05). The therapeutic effect of EA was notably superior to that of EA+inhibitor and inhibitor in increasing body mass, SOD activity, and FoxO1 and PGC-1α expression levels(P<0.05, P<0.01), and in down-regulating blood glucose, BUN, ALB and ROS levels(P<0.05, P<0.01), suggesting a reduction of the therapeutic effect of EA after administration of the inhibitor AS1842856 of FoxO1. Results of electron microscopy showed diffusely thickened and vague basement membrane, increased mesangial matrix, fused foot process, and reduced volume of endothelial cells with pykno-tic nucleus of the kidney tissue in the model group, which was obviously milder in both EA and EA+inhibitor groups particularly in the EA group. Conclusion EA increases the expression of FoxO1 and PGC-1α in the kidneys of DN rats, thereby reducing the oxidative stress response and protecting the kidneys.关键词：Diabetic nephropathy;“Biaoben” acupoints combination;Electroacupuncture;Forkhead box O1(FoxO1);Peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α)32|617|0更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) preconditioning on expression of Caspase-1, Gasdermin D(GSDMD) and interleukin-1β(IL-1β) in myocardial tissue of myocardial ischemia reperfusion injury(MIRI) rats in order to explore its underlying mechanisms in resisting MIRI. Methods Forty male rats were randomly divided into 4 groups: normal control(normal), sham operation(sham), MIRI model and EA groups. The MIRI model was established by ligation of the left anterior descending branch of the left coronary artery for 30 min and perfusion. EA(2 Hz/100 Hz, 1 mA) was applied to bilateral “Neiguan”(PC6) for 20 min, once a day for 3 consecutive days. The echocardiography was used to analyze the left ventricular end-diastolic dimension(LVEDD), left ventricular end-systolic dimension(LVESD) and left ventricular ejection fraction(LVEF, by using Teichholz formula) 4 h after modeling. The myocardial TTC staining was used to observe the proportion of the infarct area, and Western blot was used to detect the expression levels of GSDMD, Caspase-1, IL-1β proteins in the myocardium. Results Compared with the normal group, the immunoactivity of GSDMD was increased in the sham group(P<0.05). Compared with the sham group, the LVEF was significantly decreased(P<0.000 1), while the myocardial infarction area, immunoactivity of GSDMD, and the expression levels of Caspase-1, GSDMD and IL-1β proteins were considerably increased in the model group(P<0.000 1, P<0.001). In comparison with the model group, the decreased ejection fraction and the increased myocardial infarction area, and Caspase-1, GSDMD and IL-1β expression were reversed in the EA group(P<0.001, P<0.000 1,P<0.01). Conclusion EA preconditioning may ameliorate myocardial injury in MIRI rats which may be associated with its function in down-regulating the expression of myocardial Caspase-1 protein to reduce cardiomyocyte pyroptosis.关键词：Myocardial ischemia reperfusion injury;Electroacupuncture preconditioning;Pyroptosis;Gasdermin D(GSDMD);Caspase-137|790|3更新时间：2023-08-11
- 摘要：Objective To observe the effects of electroacupuncture(EA) at “Fengfu”(GV16), “Taichong”(LR3), and “Zusanli”(ST36) on the tyrosine hydroxylase(TH), nuclear factor-kappa B(NF-κB), interleukin(IL)-6, and zonula occludens-1(ZO-1) in mice with Parkinson's disease(PD), and to explore the underlying mechanism of EA in the treatment of PD. Methods C57 BL/6 mice were randomly divided into control, model, and EA groups, with 10 mice in each group. The PD model was induced by rotenone(i.g.) in mice for 28 d. EA was applied to GV16, LR3 and ST36 of mice in the EA group for 30 min, once daily for 14 d. The behavioral changes of mice in each group before and after treatment were observed and scored. The total distance traveled autonomously of mice was detected in the open field test. TH expression in the substantia nigra(SN) was measured by immunohistochemistry, and the changes in colon tissue structure were observed by HE staining. The intestinal mRNA expression of ZO-1, NF-κB, and IL-6 was detected by quantitative real-time PCR, and the colon tissue protein expression of NF-κB was detected by Western blot. The colon tissue concentration of IL-6 was detected by ELISA. Results Compared with the control group, the pre-and post-treatment behavioral scores of the model group and the pre-treatment behavioral score of the EA group were increased(P<0.01). Compared with the model group, the EA group showed reduced behavioral score after treatment(P<0.01). Compared with the control group, the model group showed reduced goblet cells and crypts and thinner muscle layer in the intestinal specimens of mice. Compared with the model group, the EA group showed intact surface villi, increased goblet cells and crypts, and thickened muscle layer. Compared with the control group, the model group exhibited reduced total distance traveled in the open field test, TH expression in SN, and ZO-1 mRNA expression(P<0.01), and elevated mRNA and protein expression of NF-κB and IL-6,and the ratio of p-NF-κB/NF-κB in the intestinal tract(P<0.01). Compared with the model group, the EA group displayed increased total distance traveled, TH expression in SN, and ZO-1 mRNA expression(P<0.01), and declining mRNA and protein expression of NF-κB and IL-6, and the ratio of p-NF-κB/NF-κB in the colon tissue(P<0.01, P<0.05). Conclusion EA at GV16, LR3, and ST36 can regulate the expression of NF-κB/IL-6, inhibit the transmission of the colon tissue inflammatory response, repair the intestinal barrier function, and potentiate the TH activity, thereby improving the behavioral performance of PD mice.关键词：Parkinson's disease;Tyrosine hydroxylase;Zonula occludens-1;Nuclear factor-kappa B;Interleukin-632|835|10更新时间：2023-08-11
- 摘要：Objective To explore the time-effect relationship of long snake moxibustion in intervening recurrent exopathogenic diseases of patients with yang-deficiency constitution in different moxibustion periods and provide a scientific basis for the selection of long snake moxibustion in preventing and treating recurrent exopathogenic diseases of patients with yang-deficiency constitution. Methods Ninety patients with yang-deficiency constitution who met the inclusion criteria of recurrent exopathogenic diseases were randomly divided into a 30 min group, a 60 min group, and a 90 min group, with 30 cases in each group. Long snake moxibustion was applied once a week from Dazhui(GV14) to Yaoshu(GV2) for different periods(30, 60, and 90 min), 12 times(12 weeks) in total. The scores of yang-deficiency constitution quality scale and Fatigue Severity Scale(FSS) before treatment, after treatment, and six months after treatment, as well as attack times of exopathogenic diseases within one year before treatment and after treatment in the three groups, were observed and recorded. Results After treatment and 6 months after treatment, the yang-deficiency quality scale scores and FSS scores of the three groups were lower than those before treatment(P<0.01), which decreased in the sequence of the 30 min group, the 90 min group, and the 60 min group(P<0.05). Within one year after treatment, the attack times of exopathogenic diseases in the three groups was lower than that within one year before treatment(P<0.01), which decreased in the sequence of the 30 min group, the 90 min group, and the 60 min group(P<0.05). Conclusion The optimal moxibustion time of long snake moxibustion on the recurrent exopathogenic diseases of patients with yang-deficiency constitution is 60 min.关键词：Long snake moxibustion;Moxibustion time;Moxibustion effect;Yang-deficiency constitution;Exopathogenic diseases28|463|0更新时间：2023-08-11
- 摘要：Objective To explore the feasibility and application value of combination regularities of acupoint Houxi(SI3) in Chinese ancient times based on latent structure model. Methods Relevant articles about SI3 for treating various diseases with acupuncture, moxibustion, acupoint application, etc. were mainly searched from book Chinese Medical Classics(5 th edition), followed by establishment of a Database of Houxi Acupoint Recipes. The Lantern 5.0 software was used to construct and analyze the latent structure model of high-frequently-used acupoints. Results A total of 46 high frequently-used acupoints contained in 240 articles of 26 medical books were collected. The top 7 acupoints are Shenmai(BL62), Hegu(LI4), Qiangu(SI2), Fengchi(GB20), Jianshi(PC5), Wangu(SI4) and Quchi(LI11) in sequence. After modeling the 46 high-frequently used adjunct acupoints, 12 latent variables(Y0-Y11) and 24 latent classes were obtained by setting the cumulative coverage threshold ratio to be 95%. According to the Bayesian information criterion(BIC) measure, the model score was-2 170.68 points. Seven comprehensive clustering models were summarized up according to the latent structure. Compared with the yin meridians, the yang meridians played a more significant role. The multiple combinations of SI3 with specific acupoints provided a reference for clinical practice. The supplementary acupoints mainly distribute in the upper and lower limbs, head, face, neck, etc. and the SI3 acupoint recipes function mainly in dredging and activating meridians and collaterals, clearing away pathologic heat and wind, improving eyesight, and relieving swelling and pain. Conclusion The latent structure model is applicable in analysis of the regularities of SI3 acupoint combination for treating some diseases. Comprehensive clustering is employed to determine the primary acupoint SI3 and adjunct acupoint matching, revealing the common regularity and logical progressive relationship between the primary and secondary points, which may be helpful for teaching, clinical and scientific research.关键词：Houxi(SI3);Acupuncture and moxibustion;Latent structure model;Acupoint combination regularity34|779|4更新时间：2023-08-11
- 摘要：Alzheimer's disease(AD), also called senile dementia, is a neurodegenerative disease, characterized by progressive memory and cognitive impairment and different degrees of behavior-mental dysfunction. Clinical trials displayed that acupuncture therapy is effective in relieving symptoms of AD patients. In recent years, many experimental studies have been conducted in SAMP8 mice to explore the underlying mechanisms of acupuncture in improving AD. Results showed that acupuncture therapy can intervene the central pathological process of AD in multiple approaches, including reducing formation of cerebral β amyloid protein and promoting its removal, intervening the phosphorylation process of Tau protein, improving mitochondrial and synaptic structure, enhancing autophagy activity, accelerating cerebral blood flow, and increasing the levels of estrogen content, and improving the learning and memory ability, etc.关键词：Acupuncture;Alzheimer's disease;SAMP8 model mice;Biological mechanisms;Review30|725|3更新时间：2023-08-11
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